Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 440,793 G→T 7.5% R444S (CGC→AGC)  yajR ← putative transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273440,7930GT 7.5% 1183.8 / 44.7 314R444S (CGC→AGC) yajRputative transporter
Reads supporting (aligned to +/- strand):  ref base G (139/150);  new base T (10/14);  total (149/165)
Fisher's exact test for biased strand distribution p-value = 6.72e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 315 total aligned reads displayed.

CGTTTTGTTCATGTCAGATGCGGCATGAACGCCTGATTCGGCCTACAAAGGTTTGTAATTCAGAAACTTTGTAGGCCTGATAAGCGTAGCGCAGCAGGCAATTCGCCGCGTTTTTTATGCCTGACGAATTGCCTGTTCTATCTCAAAGCGATTCGTCACTTTGCTGTCGATTTTCACATAAGCTGAATGTTCTTCTTCTGCAATCAACACTTCTTTGATGCCTTCAGTTTCTAGCAAACGCACTTTTAACGCCTCGTTTGCGGCAATGTTCGCCGGGATTTCAATGCGCAAACTGCTG  >  CP009273/440644‑440941
                                                                                                                                                     |                                                                                                                                                    
    ttGTTCATGTCAGATGCGGCATGAACGCCTGATTCGGCCTACAAAGGTTTGTAATTCAGAAACTTTGTAGGCCTGATAAGCGTAGCGCAGCAGGCAATTCGCCGCGTTTTTTATGCCTGACGAATTGCCTGTTCTATCTCAAAGCGATTc                                                                                                                                                  >  1:199013/1‑150 (MQ=255)
        tCATGTCAGATGCGGCATGAACGCCTGATTCGGCCTACAAAGGTTTGTAATTCAGAAACTTTGTAGGCCTGATAAGCGTAGCGCAGCAGGCAATTCGCCGCGTTTTTTATGCCTGACGAATTGCCTGTTCTATCTCAAAGCGATTCGTCa                                                                                                                                              >  2:2930799/1‑150 (MQ=255)
        tCATGTCAGATGCGGCATGAACGCCTGATTCGGCCTACAAAGGTTTGTAATTCAGAAACTTTGTAGGCCTGATAAGCGTAGCGCAGCAGGCAATTCGCCGCGTTTTTTATGCCTGACGAATTGCCTGTTCTATCTCAAAGCGATTCGTCa                                                                                                                                              >  2:3328158/1‑150 (MQ=255)
        tCATGTCAGATGCGGCATGAACGCCTGATTCGGCCTACAAAGGTTTGTAATTCAGAAACTTTGTAGGCCTGATAAGCGTAGCGCAGCAGGCAATTCGCCGCGTTTTTTATGCCTGACGAATTGCCTGTTCTATCTCAAAGCGATTCGTCa                                                                                                                                              >  1:2871844/1‑150 (MQ=255)
         cATGTAAGATGCGCCATGAACGCCTGATTCGGCCTACAAAGGTTTGTAATTCAGAAACTTAGTAGGCCTGATAAGCGTACCGCAGCAGGCAATTCGCCGCGTTTTTTATGCCTGACGAATTGCCTGTTCTATCTCAAAGCGAGTCGTCAc                                                                                                                                             >  2:4630230/1‑150 (MQ=255)
          aTGTCAGATGCGGCATGAACGCCTGATTCGGCCTACAAAGGTTTGTAATTCAGAAACTTTGTAGGCCTGATAAGCGTAGCGCAGCAGGCAATTCGCCGCGTTTTTTATGCCTGACGAATTGCCTGTTCTATCTCAAAGCGATTCGTCACt                                                                                                                                            <  2:659365/150‑1 (MQ=255)
          aTGTCAGATGCGGCATGAACGCCTGATTCGGCCTACAAAGGTTTGTAATTCAGAAACTTTGTAGGCCTGATAAGCGTAGCGCAGCAGGCAATTCGCCGCGTTTTTTATGCCTGACGAATTGCCTGTTCTATCTCAAAGCGATTCGTCACt                                                                                                                                            <  1:3891149/150‑1 (MQ=255)
             tCAGATGCGGCATGAACGCCTGATTCGGCCTACAAAGGTTTGTAATTCAGAAACTTTGTAGGCCTGATAAGCGTAGCGCAGCAGGCAATTCGCCGCGTTTTTTATGCCTGACGAATTGCCTGTTCTATCTCAAAGCGATTCGTCACTTTg                                                                                                                                         >  1:5040605/1‑150 (MQ=255)
             tCAGATGCGGCATGAACGCCTGATTCGGCCTACAAAGGTTTGTAATTCAGAAACTTTGTAGGCCTGATAAGCGTAGCGCAGCAGGCAATTCGCCGCGTTTTTTATGCCTGACGAATTGCCTGTTCTATCTCAAAGCGATTCGTCACTTTg                                                                                                                                         <  1:3836103/150‑1 (MQ=255)
              cAGATGCGGCATGAACGCCTGATTCGGCCTACAAAGGTTTGTAATTCAGAAACTTTGTAGGCCTGATAAGCGTAGCGCAGCAGGCAATTCGCCGCGTTTTTTATGCCTGACGAATTGCCTGTTCTATCTCAAAGCGATTCGTCACTTTGc                                                                                                                                        >  2:3071376/1‑150 (MQ=255)
              cAGATGCGGCATGAACGCCTGATTCGGCCTACAAAGGTTTGTAATTCAGAAACTTTGTAGGCCTGATAAGCGTAGCGCAGCAGGCAATTCGCCGCGTTTTTTATGCCTGACGAATTGCCTGTTCTATCTCAAAGCGATTCGTCACTTTGc                                                                                                                                        >  1:3398089/1‑150 (MQ=255)
               aGATGCGGCATGAACGCCTGATTCGGCCTACAAAGGTTTGTAATTCAGAAACTTTGTAGGCCTGATAAGCGTAGCGCAGCAGGCAATTCGCCGCGTTTTTTATGCCTGACGAATTGCCTGTTCTATCTCAAAGCGATTCGTCACTTTGCt                                                                                                                                       >  1:3050799/1‑150 (MQ=255)
                gATGCGGCATGAACGCCTGATTCGGCCTACCAAGGTTTGTAATTCAGAAACTTTGTAGGCCTGATAAGCGTAGCGCAGCAGGCAATTCGCCGCGTTTTTTATGCCTGACGAATTGCCTGTTCTATCTCAAAGCGATTCGTCACTTTGCTg                                                                                                                                      <  1:2102367/150‑1 (MQ=255)
                    cGGCATGAACGCCTGATTCGGCCTACAAAGGTTTGTAATTCAGAAACTTTGTAGGCCTGATAAGCGTAGCGCAGCAGGCACTTCGCCGCGTTTTTTATGCCTGACGAATTGCCTGTTCTCTCTCAAAGCGATTCGTCACTTTGCTGTCGa                                                                                                                                  >  2:2674603/1‑150 (MQ=255)
                     ggCATGAACGCCTGATTCGGCCTACAAAGGTTTGTAATTCAGAAACTTTGTAGGCCTGATAAGCGTAGCGCAGCAGGCAATTCGCCGCGTTTTTTATGCCTGACGAATTGCCTGTTCTATCTCAAAGCGATTCGTCACTTTGCTGTCGAt                                                                                                                                 >  2:3470488/1‑150 (MQ=255)
                     ggCATGAACGCCTGATTCGGCCTACAAAGGTTTGTAATTCAGAAACTTTGTAGGCCTGATAAGCGTAGCGCAGCAGGCAATTCGCCGCGTTTTTTATGCCTGACGAATTGCCTGTTCTATCTCAAAGCGATTCGTCACTTTGCTGTCGAt                                                                                                                                 <  2:1074435/150‑1 (MQ=255)
                       cATGAACGCCTGATTCGGCCTACAAAGGTTTGTAATTCAGAAACTTTGTAGGCCTGATAAGCGTAGCGCAGCAGGCAATTCGCCGCGTTTTTTATGCCTGACGAATTGCCTGTTCTATCTCAAAGCGATTCGTCACTTTGCTGTCGAttt                                                                                                                               >  2:151301/1‑150 (MQ=255)
                       cATGAACGCCTGATTCGGCCTACAAAGGTTTGTAATTCAGAAACTTTGTAGGCCTGATAAGCGTAGCGCAGCAGGCAATTCGCCGCGTTTTTTATGCCTGACGAATTGCCTGTTCTATCTCAAAGCGATTCGTCACTTTGCTGTCGAttt                                                                                                                               >  2:4990654/1‑150 (MQ=255)
                         tGAACGCCTGATTCGGCCTACAAAGGTTTGTAATTCAGAAACTTTGTAGGCCTGATAAGCGTAGCGCAGCAGGCAATTCGCCGCGTTTTTTATGCCTGACGAATTGCCTGTTCTATCTCAAAGCGATTCGTCACTTTGCTGTCGATTTTc                                                                                                                             <  2:2968101/150‑1 (MQ=255)
                         tGAACGCCTGATTCGGCCTACAAAGGTTTGTAATTCAGAAACTTTGTAGGCCTGATAAGCGTAGCGCAGCAGGCAATTCGCCGCGTTTTTTATGCCTGACCAATTGCCTGTTCTATCTCAAAGCGATTCGTCACTTTGCTGTCGATTTTc                                                                                                                             <  1:3640811/150‑1 (MQ=255)
                          gAACGCCTGATTCGGCCTACAAAGGTTTGTAATTCAGAAACTTTGTAGGCCTGATAAGCGTAGCGCAGCAGGCAATTCGCCGCGTTTTTTATGCCTGACGAATTGCCTGTTCTATCTCAAAGCGATTCGTCACTTTGCTGTCGATTTTca                                                                                                                            >  1:2812791/1‑150 (MQ=255)
                           aaCGCCTGATTCGGCCTACAAAGGTTTGTAATTCAGAAACTTTGTAGGCCTGATAAGCGTAGCGCAGCAGGCAATTCGCCGCGTTTTTTATGCCTGACGAATTGCCTGTTCTATCTCAAAGCGATTCGTCACTTTGCTGTCGATTTTcac                                                                                                                           >  1:1000343/1‑150 (MQ=255)
                           aaCGCCTGATTCGGCCTACAAAGGTTTGTAATTCAGAAACTTTGTAGGCCTGATAAGCGTAGCGCAGCAGGCAATTCGCCGCGTTTTTTATGCCTGACGAATTGCCTGTTCTATCTCAAAGCGATTCGTCACTTTGCTGTCGATTTTcac                                                                                                                           >  2:4444831/1‑150 (MQ=255)
                              gCCTGATTCGGCCTACAAAGGTTTGTAATTCAGAAACTTTGTAGGCCTGATAAGCGTAGCGCAGCAGGCAATTCGCCGCGTTTTTTATGCCTGACGAATTGCCTGTTCTATCTCAAAGCGATTCGTCACTTTGCTGTCGATTTTCACATa                                                                                                                        >  2:1878996/1‑150 (MQ=255)
                                cTGATTCGGCCTACAAAGGTTTGTAATTCAGAAACTTTGTAGGCCTGATAAGCGTAGCGCAGCAGGCAATTCGCCGCGTTTTTTATGCCTGACGAATTGCCTGTTCTATCTCAAAGCGATTCGTCACTTTGCTGTCGATTTTCACATAAg                                                                                                                      >  1:3226994/1‑150 (MQ=255)
                                  gATTCGGCCTACAAAGGTTTGTAATTCAGAAACTTTGTAGGCCTGATAAGCGTAGCGCAGCAGGCAATTCGCCGCGTTTTTTATGCCTGACGAATTGCCTGTTCTATCTCAAAGCGATTCGTCACTTTGCTGTCGATTTTCACATAAGCt                                                                                                                    >  2:4602117/1‑150 (MQ=255)
                                     tCGGCCTACAAAGGTTTGTAATTCAGAAACTTTGTAGGCCTGATAAGCGTAGCGCAGCAGGCAATTCGCCGCGTTTTTTATGCCTGACGAATTGCCTGTTCTATCTCAAAGCGATTCGTCACTTTGCTGTCGATTTTCACATAAGCTGaa                                                                                                                 <  1:1780035/150‑1 (MQ=255)
                                       ggCCTACAAAGGTTTGTAATTCAGAAACTTTGTAGGCCTGATAAGCGTAGCGCAGCAGGCAATTCGCCGCGTTTTTTATGCCTGACGAATTGCCTGTTCTATCTCAAAGCGATTCGTCACTTTGCTGTCGATTTTCACATAAGCTGAATg                                                                                                               <  2:418983/150‑1 (MQ=255)
                                        gCCTACAAAGGTTTGTAATTCAGAAACTTTGTAGGCCTGATAAGCGTAGCGCAGCAGGCAATTCGCCGCGTTTTTTATGCCTGACGAATTGCCTGTTCTATCTCAAAGCTATTCGTCACTTTGCTGTCGATTTTCACATAAGCTGAATGt                                                                                                              <  1:3546290/150‑1 (MQ=255)
                                        gCCTACAAAGGTTTGTAATTCAGAAACTTTGTAGGCCTGATAAGCGTAGCGCAGCAGGCAATTCGCCGCGTTTTTTATGCCTGACGAATTGCCTGTTCTATCTCAAAGCTATTCGTCACTTTGCTGTCGATTTTCACATAAGCTGAATGt                                                                                                              <  1:974443/150‑1 (MQ=255)
                                            aCAAAGGTTTGTAATTCAGAAACTTTGTAGGCCTGATAAGCGTAGCGCAGCAGGCAATTCGCCGCGTTTTTTATGCCTGACGAATTGCCTGTTCTATCTCAAAGCGATTCGTCACTTTGCTGTCGATTTTCACATAAGCTGAATGttctt                                                                                                          >  1:1370365/1‑150 (MQ=255)
                                            aCAAAGGTTTGTAATTCAGAAACTTTGTAGGCCTGATAAGCGTAGCGCAGCAGGCAATTCGCCGCGTTTTTTATGCCTGACGAATTGCCTGTTCTATCTCAAAGCGATTCGTCACTTTGCTGTCGATTTTCACATAAGCTGAATGttctt                                                                                                          <  1:867931/150‑1 (MQ=255)
                                              aaaGGTTTGTAATTCAGAAACTTTGTAGGCCTGATAAGCGTAGCGCAGCAGGCAATTCGCCGCGTTTTTTATGCCTGACGAATTGCCTGTTCTATCTCAAAGCTATTCGTCACTTTGCTGTCGATTTTCACATAAGCTGAATGttcttct                                                                                                        >  2:1808946/1‑150 (MQ=255)
                                              aaaGGTTTGTAATTCAGAAACTTTGTAGGCCTGATAAGCGTAGCGCAGCAGGCAATTCGCCGCGTTTTTTATGCCTGACGAATTGCCTGTTCTATCTCAAAGCGATTCGTCACTTTGCTGTCGATTTTCACATAAGCTGAATGttcttct                                                                                                        <  2:575055/150‑1 (MQ=255)
                                                 ggTTTGTAATTCAGAAACTTTGTAGGCCTGATAAGCGTAGCGCAGCAGGCAATTCGCCGCGTTTTTTATGCCTGACGAATTGCCTGTTCTATCTCAAAGCGATTCGTCACTTTGCTGTCGATTTTCACATAAGCTGAATGTtcttcttct                                                                                                     <  1:3730558/150‑1 (MQ=255)
                                                  gTTTGTAATTCAGAAACTTTGTAGGCCTGATAAGCGTAGCGCAGCAGGCAATTCGCCGCGTTTTTTATGCCTGACGAATTGCCTGTTCTATCTCAAAGCGATTCGTCACTTTGCTGTCGATTTTCACATAAGCTGAATGTTCTTCTTCTg                                                                                                    <  2:3445017/150‑1 (MQ=255)
                                                   tttGTAATTCAGAAACTTTGTAGGCCTGATAAGCGTAGCGCAGCAGGCAATTCGCCGCGTTTTTTATGCCTGACGAATTGCCTGTTCTATCTCAAAGCGATTCGTCACTTTGCTGTCGATTTTCACATAAGCTGAATGTTCTTCTTCTGc                                                                                                   <  1:2757769/150‑1 (MQ=255)
                                                     tGTAATTCAGAAACTTTGTAGGCCTGATAAGCGTAGCGCAGCAGGCAATTCGCCGCGTTTTTTATGCCTGACGAATTGCCTGTTCTATCTCAAAGCGATTCGTCACTTTGCTGTCGATTTTCACATAAGCTGAATGTTCTTCTTCTGCaa                                                                                                 >  2:2744346/1‑150 (MQ=255)
                                                     tGTAATTCAGAAACTTTGTAGGCCTGATAAGCGTAGCGCAGCAGGCAATTCGCCGCGTTTTTTATGCCTGACGAATTGCCTGTTCTATCTCAAAGCGATTCGTCACTTTGCTGTCGATTTTCACATAAGCTGAATGTTCTTCTTCTGCaa                                                                                                 >  1:1017916/1‑150 (MQ=255)
                                                     tGTAATTCAGAAACTTTGTAGGCCTGATAAGCGTAGCGCAGCAGGCAATTCGCCGCGTTTTTTATGCCTGACGAATTGCCTGTTCTATCTCAAAGCGATTCGTCACTTTGCTGTCGATTTTCACATAAGCTGAATGTTCTTCTTCTGCaa                                                                                                 >  1:2419160/1‑150 (MQ=255)
                                                       tAATTCAGAAACTTTGTAGGCCTGATAAGCGTAGCGCAGCAGGCAATTCGCCGCGTTTTTTATGCCTGACGAATTGCCTGTTCTATCTCAAAGCTATTCGTCACTTTGCTGTCGATTTTCACATAAGCTGAATGTTCTTCTTCTGCAATc                                                                                               <  2:1215915/150‑1 (MQ=255)
                                                         aTTCAGAAACTTTGTAGGCCTGATAAGCGTAGCGCAGCAGGCAATTCGCCGCGTTTTTTATGCCTGACGAATTGCCTGTTCTATCTCAAAGCGATTCGTCACTTTGCTGTCGATTTTCACATAAGCTGAATGTTCTTCTTCTGCAATCaa                                                                                             <  2:4539204/150‑1 (MQ=255)
                                                         aTTCAGAAACTTTGTAGGCCTGATAAGCGTAGCGCAGCAGGCAATTCGCCGCGTTTTTTATGCCTGACGAATTGCCTGTTCTATCTCAAAGCGATTCGTCACTTTGCTGTCGATTTTCACATAAGCTGAATGTTCTTCTTCTGCAATCaa                                                                                             <  1:4317178/150‑1 (MQ=255)
                                                         aTTCAGAAACTTTGTAGGCCTGATAAGCGTAGCGCAGCAGGCAATTCGCCGCGTTTTTTATGCCTGACGAATTGCCTGTTCTATCTCAAAGCGATTCGTCACTTTGCTGTCGATTTTCACATAAGCTGAATGTTCTTCTTCTGCAATCaa                                                                                             <  1:3171793/150‑1 (MQ=255)
                                                           tCAGAAACTTTGTAGGCCTGATAAGCGTAGCGCAGCAGGCAATTCGCCGCGTTTTTTATGCCTGACGAATTGCCTGTTCTATCTCAAAGCGATTCGTCACTTTGCTGTCGATTTTCACATAAGCTGAATGTTCTTCTTCTGCAATCAaca                                                                                           <  2:3600767/150‑1 (MQ=255)
                                                            cAGAAACTTTGTAGGCCTGATAAGCGTAGCGCAGCAGGCAATTCGCCGCGTTTTTTATGCCTGACGAATTGCCTGTTCTATCTCAAAGCGATTCGTCACTTTGCTGTCGATTTTCACATAAGCTGAATGTTCTTCTTCTGCAATCAacac                                                                                          >  1:2287173/1‑150 (MQ=255)
                                                            cAGAAACTTTGTAGGCCTGATAAGCGTAGCGCAGCAGGCAATTCGCCGCGTTTTTTATGCCTGACGAATTGCCTGTTCTATCTCAAAGCGATTCGTCACTTTGCTGTCGATTTTCACATAAGCTGAATGTTCTTCTTCTGCAATCAacac                                                                                          >  1:4970437/1‑150 (MQ=255)
                                                            cAGAAACTTTGTAGGCCTGATAAGCGTAGCGCAGCAGGCAATTCGCCGCGTTTTTTATGCCTGACGAATTGCCTGTTCTATCTCAAAGCGATTCGTCACTTTGCTGTCGATTTTCACATAAGCTGAATGTTCTTCTTCTGCAATCAacac                                                                                          >  2:2856008/1‑150 (MQ=255)
                                                            cAGAAACTTTGTAGGCCTGATAAGCGTAGCGCAGCAGGCAATTCGCCGCGTTTTTTATGCCTGACGAATTGCCTGTTCTATCTCAAAGCGATTCGTCACTTTGCTGTCGATTATCACATAAGCTGAATGTTCTTCTTCTGCAATCAacac                                                                                          >  2:2784637/1‑150 (MQ=255)
                                                              gAAACTTTGTAGGCCTGATAAGCGTAGCGCAGCAGGCAATTCGCCGCGTTTTTTATGCCTGACGAATTGCCTTTTCTATCTCAAAGCGATTCGTCACTTTGCTGTCGATTTTCACATAAGCTGAATGTTCTTCTTCTGCAATCAACActt                                                                                        <  2:3653483/150‑1 (MQ=255)
                                                                  cTTTGTAGGCCTGATAAGCGTAGCGCAGCAGGCAATTCGCCGCGTTTTTTATGCCTGACGAATTGCCTGTTCTATCTCAAAGCGATTCGTCACTTTGCTGTCGATTTTCACATAAGCTGAATGTTCTTCTTCTGCAATCAACACTTCttt                                                                                    <  2:4889191/150‑1 (MQ=255)
                                                                   tttGTAGGCCTGATAAGCGTAGCGCAGCAGGCAATTCGCCGCGTTTTTTATGCCTGACGAATTGCCTGTTCTATCTCAAAGCGATTCGTCACTTTGCTGTCGATTTTCACATAAGCTGAATGTTCTTCTTCTGCAATCAACACTTCTTTg                                                                                   <  2:2027510/150‑1 (MQ=255)
                                                                    ttGTAGGCCTGATAAGCGTAGCGCAGCAGGCAATTCGCCGCGTTTTTTATGCCTGACGAATTGCCTGTTCTATCTCAAAGCGATTCGTCACTTTGCTGTCGATTTTCACATAAGCTGAATGTTCTTCTTCTGCAATCAACACTTCTTTGa                                                                                  <  1:628019/150‑1 (MQ=255)
                                                                           ccTGATAAGCGTAGCGCAGCAGGCAATTCGCCGCGTTTTTTATGCCTGACGAATTGCCTGTTCTATCTCAAAGCGATTCGTCACTTTGCTGTCGATTTTCACATAAGCTGAATGTTCTTCTTCTGCAATCAACACTTCTTTGATGCCTTc                                                                           <  1:4579465/150‑1 (MQ=255)
                                                                            cTGATAAGCGTAGCGCAGCAGGCAATTCGCCGCGTTTTTTATGCCTGACGAATTGCCTGTTCTATCTCAAAGCGATTCGTCACTTTGCTGTCGATTTTCACATAAGCTGAATGTTCTTCTTCTGCAATCAACACTTCTTTGATGCCTTCa                                                                          <  2:3798199/150‑1 (MQ=255)
                                                                             tGATAAGCGTAGCGCAGCAGGCAATTCGCCGCGTTTTTTATGCCTGACGAATTGCCTGTTCTATCTCAAAGCGATTCGTCACTTTGCTGTCGATTTTCACATAAGCTGAATGTTCTTCTTCTGCAATCAACACTTCTTTGATGCCTTCAg                                                                         <  1:4162533/150‑1 (MQ=255)
                                                                               aTAAGCGTAGCGCAGCAGGCAATTCGCCGCGTTTTTTATGCCTGACGAATTGCCTGTTCTATCTCAAAGCGATTCGTCACTTTGCTGTCGATTTTCACATAAGCTGAATGTTCTTCTTCTGCAATCAACACTTCTTTGATGCCTTCAGtt                                                                       <  2:1117945/150‑1 (MQ=255)
                                                                                   gCGTAGCGCAGCAGGCAATTCGCCGCGTTTTTTATGCCTGACGAATTGCCTGTTCTATCTCAAAGCTATTCGTCACTTTGCTGTCGATTTTCACATAAGCTGAATGTTCTTCTTCTGCAATCAACACTTCTTTGATGCCTTCAGTTTCTa                                                                   <  1:4214885/150‑1 (MQ=255)
                                                                                   gCGTAGCGCAGCAGGCAATTCGCCGCGTTTTTTATGCCTGACGAATTGCCTGTTCTATCTCAAAGCGATTCGTCACTTTGCTGTCGATTTTCACATAAGCTGAATGTTCTTCTTCTGCAATCAACACTTCTTTGATGCCTTCAGTTTCTa                                                                   <  2:4486055/150‑1 (MQ=255)
                                                                                     gTAGCGCAGCAGGCAATTCGCCGCGTTTTTTATGCCTGACGAATTGCCTGTTCTATCTCAAAGCGATTCGTCACTTTGCTGTCGATTTTCACATAAGCTGAATGTTCTTCTTCTGCAATCAACACTTCTTTGATGCCTTCAGTTTCTAGc                                                                 <  2:1563536/150‑1 (MQ=255)
                                                                                      tAGCGCAGCAGGCAATTCGCCGCGTTTTTTATGCCTGACGAATTGCCTGTTCTATCTCAGAGCGATTCGTCACTTTGCTGTCGATTTTCACATAAGCTGAATGTTCTTCTTCTGCAATCACCACTTCTTTGATGCCTTCAGTTTCTAGCa                                                                <  2:2500838/150‑1 (MQ=255)
                                                                                      tAGCGCAGCAGGCAATTCGCCGCGTTTTTTATGCCTGACGAATTGCCTGTTCTATCTCAAAGCGATTCGTCACTTTGCTGTCGATTTTCACATAAGCTGAATGTTCTTCTTCTGCAATCAACACTTCTTTGATGCCTTCAGTTTCTAGCa                                                                >  2:4022799/1‑150 (MQ=255)
                                                                                        gcgcAGCAGGCAATTCGCCGCGTTTTTTATGCCTGACGAATTGCCTGTTCTATCTCAAAGCGATTCGTCACTTTGCTGTCGATTTTCACATAAGCTGAATGTTCTTCTTCTGCAATCAACACTTCTTTGATGCCTTCAGTTTCTAGCaaa                                                              >  2:854040/1‑150 (MQ=255)
                                                                                         cgcAGCAGGCAATTCGCCGCGTTTTTTATGCCTGACGAATTGCCTGTTCTATCTCAAAGCGATTCGTCACTTTGCTGTCGATTTTCACATAAGCTGAATGTTCTTCTTCTGCAATCAACACTTCTTTGATGCCTTCAGTTTCTAGCAAAc                                                             <  1:161344/150‑1 (MQ=255)
                                                                                          gcagcaGGCAATTCGCCGCGTTTTTTATGCCTGACGAATTGCCTGTTCTATCTCAAAGCGATTCGTCACTTTGCTGTCGATTTTCACATAAGCTGAATGTTCTTCTTCTGCAATCAACACTTCTTTGATGCCTTCAGTTTCTAGCAAACg                                                            <  1:70243/150‑1 (MQ=255)
                                                                                            agcagGCAATTCGCCGCGTTTTTTATGCCTGACGAATTGCCTGTTCTATCTCAAAGCTATTCGTCACTTTGCTGTCGATTTTCACATAAGCTGAATGTTCTTCTTCTGCAATCAACACTTCTTTGATGCCTTCAGTTTCTAGCAAACGCa                                                          <  1:4415772/150‑1 (MQ=255)
                                                                                            agcagGCAATTCGCCGCGTTTTTTATGCCTGACGAATTGCCTGTTCTATCTCAAAGCGATTCGTCACTTTGCTGTCGATTTTCACATAAGCTGAATGTTCTTCTTCTGCAATCAACACTTCTTTGATGCCTTCAGTTTCTAGCAAACGCa                                                          >  1:396277/1‑150 (MQ=255)
                                                                                               agGCAATTCGCCGCGTTTTTTATGCCTGACGAATTGCCTGTTCTATCTCAAAGCGATTCGTCACTTTGCTGTCGATTTTCACATAAGCTGAATGTTCTTCTTCTGCAATCAACACTTCTTTGATGCCTTCAGTTTCTAGCAAACGCACtt                                                       <  2:4354953/150‑1 (MQ=255)
                                                                                                  cAATTCGCCGCGTTTTTTATGCCTGACGAATTGCCTGTTCTATCTCAAAGCGATTCGTCACTTTGCTGTCGATTTTCACATAAGCTGAATGTTCTTCTTCTGCAATCAACACTTCTTTGATGCCTTCAGTTTCTATCAAACGCACTTTTa                                                    >  1:3012636/1‑150 (MQ=255)
                                                                                                  cAATTCGCCGCGTTTTTTATGCCTGACGAATTGCCTGTTCTATCTCAAAGCGATTCGTCACTTTGCTGTCGATTTTCACATAAGCTGAATGTTCTTCTTCTGCAATCAACACTTCTTTGATGCCTTCAGTTTCTAGCAAACGCACTTTTa                                                    >  2:2230183/1‑150 (MQ=255)
                                                                                                  cAATTCGCCGCGTTTTTTATGCCTGACGAATTGCCTGTTCTATCTCAAAGCGATTCGTCACTTTGCTGTCGATTTTCACATAAGCTGAATGTTCTTCTTCTGCAATCAACACTTCTTTGATGCCTTCAGTTTCTAGCAAACGCACTTTTa                                                    <  1:1108846/150‑1 (MQ=255)
                                                                                                    aTTCGCCGCGTTTTTTATGCCTGACGAATTGCCTGTTCTATCTCAAAGCGATTCGTCACTTTGCTGTCGATTTTCACATAAGCTGAATGTTCTTCTTCTGCAATCAACACTTCTTTGATGCCTTCAGTTTCTAGCAAACGCACTTTTAAc                                                  <  1:931947/150‑1 (MQ=255)
                                                                                                    aTTCGCCGCGTTTTTTATGCCTGACGAATTGCCTGTTCTATCTCAAAGCGATTCGTCACTTTGCTGTCGATTTTCACATAAGCTGAATGTTCTTCTTCTGCAATCAACACTTCTTTGATGCCTTCAGTTTCTAGCAAACGCACTTTTAAc                                                  <  2:3894895/150‑1 (MQ=255)
                                                                                                        gccgcGTTTTTTATGCCTGACGAATTGCCTGTTCTATCTCAAAGCGATTCGTCACTTTGCTGTCGATTTTCACATAAGCTGAATGTTCTTCTTCTGCAATCAACACTTCTTTGATGCCTTCAGTTTCTAGCAAACGCACTTTTAACGCCt                                              <  1:3328158/150‑1 (MQ=255)
                                                                                                         ccgcGTTTTTTATGCCTGACGAATTGCCTGTTCTATCTCAAAGCGATTCGTCACTTTGCTGTCGATTTTCACATAAGCTGAATGTTCTTCTTCTGCAATCAACACTTCTTTGATGCCTTCAGTTTCTAGCAAACGCACTTTTAACGCCTc                                             >  1:3287119/1‑150 (MQ=255)
                                                                                                              ttttttATGCCTGACGAATTGCCTGTTCTATCTCAAAGCGATTCGTCACTTTGCTGTCGATTTTCACATAAGCTGAATGTTCTTCTTCTGCAATCAACACTTCTTTGATGCCTTCAGTTTCTAGCAAACGCACTTTTAACGCCTCGTTTg                                        >  1:2517427/1‑150 (MQ=255)
                                                                                                               tttttATGCCTGACGAATTGCCTGTTCTATCTCAAAGCGATTCGTCACTTTGCTGTCGATTTTCACATAAGCTGAATGTTCTTCTTCTGCAATCAACACTTCTTTGATGCCTTCAGTTTCTAGCAAACGCACTTTTAACGCCTCGTTTGc                                       >  2:1147807/1‑150 (MQ=255)
                                                                                                                ttttATGCCTGACGAATTGCCTGTTCTATCTCAAAGCGATTCGTCACTTTGCTGTCGATTTTCACATAAGCTGAATGTTCTTCTTCTGCAATCAACACTTCTTTGATGCCTTCAGTTTCTAGCAAACGCACTTTTAACGCCTCGTTTGCg                                      <  1:2311156/150‑1 (MQ=255)
                                                                                                                  ttATGCCTGACGAATTGCCTGTTCTATCTCAAAGCGATTCGTCACTTTGCTGTCGATTTTCACATAAGCTGAATGTTCTTCTTCTGCAATCAACACTTCTTTGATGCCTTCAGTTTCTAGCAAACGCACTTTTAACGCCTCGTTTGCGGc                                    >  1:1520994/1‑150 (MQ=255)
                                                                                                                    aTGCCTGACGAATTGCCTGTTCTATCTCAAAGCGATTCGTCACTTTGCTGTCGATTTTCACATAAGCTGAATGTTCTTCTTCTGCAATCAACACTTCTTTGATGCCTTCAGTTTCTAGCAAACGCACTTTTAACGCCTCGTTTGCGGCaa                                  >  2:4942397/1‑150 (MQ=255)
                                                                                                                       ccTGACGAATTGCCTGTTCTATCTCAAAGCGATTCGTCACTTTGCTGTCGATTTTCACATAAGCTGAATGTTCTTCTTCTGCAATCAACACTTCTTTGATGCCTTCAGTTTCTAGCAAACGCACTTTTAACGCCTCGTTTGCGGCAATGt                               >  1:3130153/1‑150 (MQ=255)
                                                                                                                          gACGAATTGCCTGTTCTATCTCAAAGCGATTCGTCACTTTGCTGTCGATTTTCACATAAGCTGAATGTTCTTCTTCTGCAATCAACACTTCTTTGATGCCTTCAGTTTCTAGCAAACGCACTTTTAACGCCTCGTTTGCGGCAATGTTCg                            <  2:4558786/150‑1 (MQ=255)
                                                                                                                              aaTTGCCTGTTCTATCTCAAAGCGATTCGTCACTTTGCTGTCGATTTTCACATAAGCTGAGTGTTCTTCTTCTGCAATCAACACTTCTTTGATGCCTTCAGTTTCTAGCAAACGCACTTTTAACGCCTCGTTTGCGGCAATGTTCGCCgg                        <  2:82283/150‑1 (MQ=255)
                                                                                                                               aTTGCCTGTTCTATCTCAAAGCGATTCGTCACTTTGCTGTCGATTTTCACATAAGCTGAATGTTCTTCTTCTGCAATCAACACTTCTTTGATGCCTTCAGTTTCTAGCAAACGCACTTTTAACGCCTCGTTTGCGGCAATGTTCGCCggg                       <  1:1260077/150‑1 (MQ=255)
                                                                                                                                 tGCCTGTTCTATCTCAAAGCGATTCGTCACTTTGCTGTCGATTTTCACATAAGCTGAATGTTCTTCTTCTGCAATCAACACTTCTTTGATGCCTTCAGTTTCTAGCAAACGCACTTTTAACGCCTCGTTTGCGGCAATGTTCGCCGGGAt                     >  1:4850139/1‑150 (MQ=255)
                                                                                                                                     tGTTCTATCTCAAAGCGATTCGTCACTTTGCTGTCGATTTTCACATAAGCTGAATGTTCTTCTTCTGCAATCAACACTTCTTTGATGCCTTCAGTTTCTAGCAAACGCACTTTTAACGCCTCGTTTGCGGCAATGTTCGCCGGGATTTCa                 >  1:4935393/1‑150 (MQ=255)
                                                                                                                                      gTTCTATCTCAAAGCGATTCGTCACTTTGCTGTCGATTTTCACATAAGCTGAATGTTCTTCTTCTGCAATCAACACTTCTTTGATGCCTTCAGTTTCTAGCAAACGCACTTTTAACGCCTCGTTTGCGGCAATGTTCGCCGGGATTTCaa                <  2:770884/150‑1 (MQ=255)
                                                                                                                                       ttCTATCTCAAAGCTATTCGTCACTTTGCTGTCGATTTTCACATAAGCTGAATGTTCTTCTTCTGCAATCAACACTTCTTTGATGCCTTCAGTTTCTAGCAAACGCACTTTTAACGCCTCGTTTGCGGCAATGTTCGCCGGGATTTCAAt               >  2:4747010/1‑150 (MQ=255)
                                                                                                                                       ttCTATCTCAAAGCGATTCGTCACTTTGCTGTCGATTTTCACATAAGCTGAATGTTCTTCTTCTGCAATCAACACTTCTTTGATGCCTTCAGTTTCTAGCAAACGCACTTTTAACGCCTCGTTTGCGGCAATGTTCGCCGGGATTTCAAt               >  1:4032333/1‑150 (MQ=255)
                                                                                                                                        tCTATCTCAAAGCGATTCGTCACTTTGCTGTCGATTTTCACATAAGCTGAATGTTCTTCTTCTGCAATCAACACTTCTTTGATGCCTTCAGTTTCTAGCAAACGCACTTTTAACGCCTCGTTTGCGGCAATGTTCGCCGGGATTTCAATg              >  2:1685708/1‑150 (MQ=255)
                                                                                                                                         cTATCTCAAAGCGATTCGTCACTTTGCTGTCGATTTTCACATAAGCTGAATGTTCTTCTTCTGCAATCAACACTTCTTTGATGCCTTCAGTTTCTAGCAAACGCACTTTTAACGCCTCGTTTGCGGCAATGTTCGCCGGGATTTCAATgc             <  2:2517427/150‑1 (MQ=255)
                                                                                                                                          tATCTCAAAGCGATTCGTCACTTTGCTGTCGATTTTCACATAAGCTGAATGTTCTTCTTCTGCAATCAACACTTCTTTGATGCCTTCAGTTTCTAGCAAACGCACTTTTAACGCCTCGTTTGCGGCAATGTTCGCCGGGATTTCAATgcg            >  1:3078458/1‑150 (MQ=255)
                                                                                                                                           aTCTCAAAGCGATTCGTCACTTTGCTGTCGATTTTCACATAAGCTGAATGTTCTTCTTCTGCAATCAACACTTCTTTGATGCCTTCAGTTTCTAGCAAACGCACTTTTAACGCCTCGTTTGCGGCAATGTTCGCCGGGATTTCAATgcgc           >  2:3238099/1‑150 (MQ=255)
                                                                                                                                            tctcAAAGCGATTCGTCACTTTGCTGTCGATTTTCACATAAGCTGAATGTTCTTCTTCTGCAATCAACACTTCTTTGATGCCTTCAGTTTCTAGCAAACGCACTTTTAACGCCTCGTTTGCGGCAATGTTCGCCGGGATTTCAATGCGCa          >  1:1862456/1‑150 (MQ=255)
                                                                                                                                              tcAAAGCGATTCGTCACTTTGCTGTCGATTTTCACATAAGCTGAATGTTCTTCTTCTGCAATCAACACTTCTTTGATGCCTTCAGTTTCTAGCAAACGCACTTTTAACGCCTCGTTTGCGGCAATGTTCGCCGGGATTTCAATGCGCaaa        >  1:4225072/1‑150 (MQ=255)
                                                                                                                                               cAAAGCGATTCGTCACTTTGCTGTCGATTTTCACATAAGCTGAATGTTCTTCTTCTGCAATCAACACTTCTTTGATGCCTTCAGTTTCTAGCAAACGCACTTTTAACGCCTCGTTTGCGGCAATGTTCGCCGGGATTTCAATGCGCAAAc       >  2:2594328/1‑150 (MQ=255)
                                                                                                                                               cAAAGCGATTCGTCACTTTGCTGTCGATTTTCACATAAGCTGAATGTTCTTCTTCTGCAATCAACACTTCTTTGATGCCTTCAGTTTCTAGCAAACGCACTTTTAACGCCTCGTTTGCGGCAATGTTCGCCGGGATTTCAATGCGCAAAc       >  2:705296/1‑150 (MQ=255)
                                                                                                                                                aaaGCGATTCGTCACTTTGCTGTCGATTTTCACATAAGCTGAATGTTCTTCTTCTGCAATCAACACTTCTTTGATGCCTTCAGTTTCTAGCAAACGCACTTTTAACGCCTCGTTTGCGGCAATGTTCGCCGGGATTTCAATGCGCAAAct      >  1:2959719/1‑150 (MQ=255)
                                                                                                                                                   gCGATTCGTCACTTTGCTGTCGATTTTCACATAAGCTGAATGTTCTTCTTCTGCAATCAACACTTCTTTGATGCCTTCAGTTTCTAGCAAACGCACTTTTAACGCCTCGTTTGCGGCAATGTTCGCCGGGATTTCAATGCGCAAActgct   <  2:1370944/150‑1 (MQ=255)
                                                                                                                                                    cGATTCGTCACTTTGCTGTCGATTTTCACATAAGCTGAATGTTCTTCTTCTGCAATCAACACTTCTTTGATGCCTTCAGTTTCTAGCAAACGCACTTTTAACGCCTCGTTTGCGGCAATGTTCGCCGGGATTTCAATGCGCAAActgctg  <  2:2331596/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
CGTTTTGTTCATGTCAGATGCGGCATGAACGCCTGATTCGGCCTACAAAGGTTTGTAATTCAGAAACTTTGTAGGCCTGATAAGCGTAGCGCAGCAGGCAATTCGCCGCGTTTTTTATGCCTGACGAATTGCCTGTTCTATCTCAAAGCGATTCGTCACTTTGCTGTCGATTTTCACATAAGCTGAATGTTCTTCTTCTGCAATCAACACTTCTTTGATGCCTTCAGTTTCTAGCAAACGCACTTTTAACGCCTCGTTTGCGGCAATGTTCGCCGGGATTTCAATGCGCAAACTGCTG  >  CP009273/440644‑440941

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑