Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 441,811 A→C 14.0% I104M (ATT→ATG)  yajR ← putative transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273441,8110AC14.0% 805.3 / 137.6 322I104M (ATT→ATG) yajRputative transporter
Reads supporting (aligned to +/- strand):  ref base A (148/129);  new base C (27/18);  total (175/147)
Fisher's exact test for biased strand distribution p-value = 4.26e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.80e-01

Only 100 of 336 total aligned reads displayed.

GCACCATCGCAATGGCAAAGGTAATGCCAAAGCTCACGCCGATAAACGCCATCGCTTTGGTGCGGTTTTGTTCGCGCGTGAGATCGGAAAGCAGCGCCATAACGGCGGCGGCAATTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATAATTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCTACCGGCGGCAAACACCGCCAGCCCACCGACAATTAATGGTTTGCGACCAATGCGGTCGGAAAGCAGGCCAAACGGAATCTGAAAAACGGCCTGAGTCAGACCATAAATACC  >  CP009273/441663‑441960
                                                                                                                                                    |                                                                                                                                                     
 cACCATCGCAATGGCAAAGGTAATGCCAAAGCTCACGCCGATAAACGCCATCGCTTTGGTGCGGTTTTGTTCGCGCGTGAGATCGGAAAGCAGCGCCATAACGGCGGCGGCAATTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGaataat                                                                                                                                                     <  2:2522796/150‑1 (MQ=255)
  aCCATCGCAATGGCAAAGGTAATGCCAAAGCTCACGCCGATAAACGCCATCGCTTTGGTGCGGTTTTGTTCGCGCGTGAGATCGGAAAGCAGCGCCATAACGGCGGCGGCAATTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATAAtt                                                                                                                                                    <  2:5020028/150‑1 (MQ=255)
      tCGCAATGGCAAAGGTAATGCCAAAGCTCACGCCGATAAACGCCATCGCTTTGGTGCGGTTTTGTTCGCGCGTGAGATCGGAAAGCAGCGCCATAACGGCGGCGGCAATTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATCATTcccc                                                                                                                                                >  2:2345616/1‑150 (MQ=255)
      tCGCAATGGCAAAGGTAATGCCAAAGCTCACGCCGATAAACGCCATCGCTTTGGTGCGGTTTTGTTCGCGCGTGAGATCGGAAAGCAGCGCCATAACGGCGGCGGCAATTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATAATTcccc                                                                                                                                                >  2:3475568/1‑150 (MQ=255)
       cGCAATGGCAAAGGTAATGCCAAAGCTCACGCCGATAAACGCCATCGCTTTGGTGCGGTTTTGTTCGCGCGTGAGATCGGAAAGCAGCGCCATAACGGCGGCGGCAATTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATAATTCCCCa                                                                                                                                               <  2:3918392/150‑1 (MQ=255)
       cGCAATGGCAAAGGTAATGCCAAAGCTCACGCCGATAAACGCCATCGCTTTGGTGCGGTTTTGTTCGCGCGTGAGATCGGAAAGCAGCGCCATAACGGCGGCGGCAATTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATAATTCCCCa                                                                                                                                               <  1:3126155/150‑1 (MQ=255)
          aaTGGCAAAGGTAATGCCAAAGCTCACGCCGATAAACGCCATCGCTTTGGTGCGGTTTTGTTCGCGCGTGAGATCGGAAAGCAGCGCCATAACGGCGGCGGCAATTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATAATTCCCCAGAt                                                                                                                                            >  2:3725934/1‑150 (MQ=255)
            tGGCAAAGGTAATGCCAAAGCTCACGCCGATAAACGCCATCGCTTTGGTGCGGTTTTGTTCGCGCGTGAGATCGGAAAGCAGCGCCATAACGGCGGCGGCAATTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATCATTCCCCAGATgg                                                                                                                                          >  1:2930977/1‑150 (MQ=255)
            tGGCAAAGGTAATGCCAAAGCTCACGCCGATAAACGCCATCGCTTTGGTGCGGTTTTGTTCGCGCGTGAGATCGGAAAGCAGCGCCATAACGGCGGCGGCAATTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATAATTCCCCAGATgg                                                                                                                                          >  1:1881632/1‑150 (MQ=255)
             ggCAAAGGTAATGCCAAAGCTCACGCCGATAAACGCCATCGCTTTGGTGCGGTTTTGTTCGCGCGTGAGATCGGAAAGCAGCGCCATAACGGCGGCGGCAATTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATAATTCCCCAGATGGa                                                                                                                                         >  2:1838540/1‑150 (MQ=255)
                 aaGGTAATGCCAAAGCTCACGCCGATAAACGCCATCGCTTTGGTGCGGTTTTGTTCGCGCGTGAGATCGGAAAGCAGCGCCATAACGGCGGCGGCAATTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATAATTCCCCAGATGGAGTCg                                                                                                                                     <  1:695447/150‑1 (MQ=255)
                 aaGGTAATGCCAAAGCTCACGCCGATAAACGCCATCGCTTTGGTGCGGTTTTGTTCGCGCGTGAGATCGGAAAGCAGCGCCATAACGGCGGCGGCAATTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATAATTCCCCAGATGGAGTCg                                                                                                                                     <  1:4300045/150‑1 (MQ=255)
                  aGGTAATGCCAAAGCTCACGCCGATAAACGCCATCGCTTTGGTGCGGTTTTGTTCGCGCGTGAGATCGGAAAGCAGCGCCATAACGGCGGCGGCAATTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATCATTCCCCAGATGGAGTCgg                                                                                                                                    <  1:1081761/150‑1 (MQ=255)
                       aTGCCAAAGCTCACGCCGATAAACGCCATCGCTTTGGTGCGGTTTTGTTCGCGCGTGAGATCGGAAAGCAGCGCCATAACGGCGGCGGCAATTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATCATTCCCCAGATGGAGTCGGAAAgc                                                                                                                               <  1:1845159/150‑1 (MQ=255)
                          ccAAAGCTCACGCCGATAAACGCCATCGCTTTGGTGCGGTTTTGTTCGCGCGTGAGATCGGAAAGCAGCGCCATAACGGCGGCGGCAATTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATCATTCCCCAGATGGAGTCGGAAAGCGCa                                                                                                                            <  2:620901/150‑1 (MQ=255)
                          ccAAAGCTCACGCCGATAAACGCCATCGCTTTGGTGCGGTTTTGTTCGCGCGTGAGATCGGAAAGCAGCGCCATAACGGCGGCGGCAATTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATAATTCCCCAGATGGAGTCGGAAAGCGCa                                                                                                                            <  2:2828721/150‑1 (MQ=255)
                            aaaGCTCACGCCGATAAACGCCATCGCTTTGGTGCGGTTTTGTTCGCGCGTGAGATCGGAAAGCAGCGCCATAACGGCGGCGGCAATTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATAATTCCCCAGATGGAGTCGGAAAGCGCAGc                                                                                                                          >  1:1159771/1‑150 (MQ=255)
                            aaaGCTCACGCCGATAAACGCCATCGCTTTGGTGCGGTTTTGTTCGCGCGTGAGATCGGAAAGCAGCGCCATAACGGCGGCGGCAATTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATAATTCCCCAGATGGAGTCGGAAAGCGCAGc                                                                                                                          >  1:3949753/1‑150 (MQ=255)
                             aaGCTCACGCCGATAAACGCCATCGCTTTGGTGCGGTTTTGTTCGCGCGTGAGATCGGAAAGCAGCGCCATAACGGCGGCGGCAATTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATAATTCCCCAGATGGAGTCGGAAAGCGCAGCg                                                                                                                         <  2:775990/150‑1 (MQ=255)
                                cTCACGCCGATAAACGCCATCGCTTTGGTGCGGTTTTGTTCGCGCGTGAGATCGGAAAGCAGCGCCATAACGGCGGCGGCAATTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATAATTCCCCAGATGGAGTCGGAAAGCGCAGCGATa                                                                                                                      >  2:184974/1‑150 (MQ=255)
                                cTCACGCCGATAAACGCCATCGCTTTGGTGCGGTTTTGTTCGCGCGTGAGATCGGAAAGCAGCGCCATAACGGCGGCGGCAATTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATAATTCCCCAGATGGAGTCGGAAAGCGCAGCGATa                                                                                                                      >  2:3166206/1‑150 (MQ=255)
                                 tCACGCCGATAAACGCCATCGCTTTGGTGCGGTTTTGTTCGCGCGTGAGATCGGAAAGCAGCGCCATAACGGCGGCGGCAATTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATAATTCCCCAGATGGAGTCGGAAAGCGCAGCGATaa                                                                                                                     >  2:3016386/1‑150 (MQ=255)
                                  cACGCCGATAAACGCCATCGCTTTGGTGCGGTTTTGTTCGCGCGTGAGATCGGAAAGCAGCGCCATAACGGCGGCGGCAATTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATAATTCCCCAGATGGAGTCGGAAAGCGCAGCGATAAc                                                                                                                    <  2:3229938/150‑1 (MQ=255)
                                   aCGCCGATAAACGCCATCGCTTTGGTGCGGTTTTGTTCGCGCGTGAGATCGGAAAGCAGCGCCATAACGGCGGCGGCAATTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATAATTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACg                                                                                                                   >  1:4388546/1‑150 (MQ=255)
                                    cGCCGATAAACGCCATCGCTTTGGTGCGGTTTTGTTCGCGCGTGAGATCGGAAAGCAGCGCCATAACGGCGGCGGCAATTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATAATTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGc                                                                                                                  >  1:2007272/1‑150 (MQ=255)
                                      ccGATAAACGCCATCGCTTTGGTGCGGTTTTGTTCGCGCGTGAGATCGGAAAGCAGCGCCATAACGGCGGCGGCAATTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGACTAATTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCTa                                                                                                                >  2:5145496/1‑150 (MQ=255)
                                      ccGATAAACGCCATCGCTTTGGTGCGGTTTTGTTCGCGCGTGAGATCGGAAAGCAGCGCCATAACGGCGGCGGCAATTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATAATTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCTa                                                                                                                >  1:1118403/1‑150 (MQ=255)
                                       cGATAAACGCCATCGCTTTGGTGCGGTTTTGTTCGCGCGTGAGATCGGAAAGCAGCGCCATAACGGCGGCGGCAATTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATAATTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCTAc                                                                                                               <  1:2470566/150‑1 (MQ=255)
                                        gATAAACGCCATCGCTTTGGTGCGGTTTTGTTCGCGCGTGAGATCGGAAAGCAGCGCCATAACGGCGGCGGCAATTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATAATTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCTAcc                                                                                                              <  1:1359123/150‑1 (MQ=255)
                                         aTAAACGCCATCGCTTTGGTGCGGTTTTGTTCGCGCGTGAGATCGGAAAGCAGCGCCATAACGGCGGCGGCAATTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATAATTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCTACcg                                                                                                             >  1:1706829/1‑150 (MQ=255)
                                         aTAAACGCCATCGCTTTGGTGCGGTTTTGTTCGCGCGTGAGATCGGAAAGCAGCGCCATAACGGCGGCGGCAATTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATAATTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCTACcg                                                                                                             <  2:713955/150‑1 (MQ=255)
                                         aTAAACGCCATCGCTTTGGTGCGGTTTTGTTCGCGCGTGAGATCGGAAAGCAGCGCCATAACGGCGGCGGCAATTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATAATTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCTACcg                                                                                                             >  1:3629301/1‑150 (MQ=255)
                                          tAAACGCCATCGCTTTGGTGCGGTTTTGTTCGCGCGTGAGATCGGAAAGCAGCGCCATAACGGCGGCGGCAATTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATAATTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCTACcgg                                                                                                            >  2:2245469/1‑150 (MQ=255)
                                           aaaCGCCATCGCTTTGGTGCGGTTTTGTTCGCGCGTGAGATCGGAAAGCAGCGCCATAACGGCGGCGGCAATTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATCATTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCTACcggc                                                                                                           >  2:4317665/1‑150 (MQ=255)
                                            aaCGCCATCGCTTTGGTGCGGTTTTGTTCGCGCGTGAGATCGGAAAGCAGCGCCATAACGGCGGCGGCAATTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATAATTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCTACcggcg                                                                                                          >  2:48638/1‑150 (MQ=255)
                                             aCGCCATCGCTTTGGTGCGGTTTTGTTCGCGCGTGAGAGCGGAAAGCAGCGCCATAACGGCGGCGGCAATTGCTCCAGAGCCGTGTAGCGCCCGGCCCCGAATAAGTCCCCAGAGGGAGGCGGACAGCGCAGCGATCCCGCGCCcggcgg                                                                                                         >  2:3438594/1‑150 (MQ=255)
                                               gCCATCGCTTTGGTGCGGTTTTGTTCGCGCGTGAGATCGGAAAGCAGCGCCATAACGGCGGCGGCAATTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATAATTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCTACCGGCGGCa                                                                                                       >  2:4253682/1‑150 (MQ=255)
                                               gCCATCGCTTTGGTGCGGTTTTGTTCGCGCGTGAGATCGGAAAGCAGCGCCATAACGGCGGCGGCAATTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATAATTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCTACCGGCGGCa                                                                                                       >  2:2772347/1‑150 (MQ=255)
                                                ccATCGCTTTGGTGCGGTTTTGTTCGCGCGTGAGATCGGAAAGCAGCGCCATAACGGCGGCGGCAATTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATAATTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCTACCGGCGGCaa                                                                                                      <  2:4728477/150‑1 (MQ=255)
                                                 cATCGCTTTGGTGCGGTTTTGTTCGCGCGTGAGATCGGAAAGCAGCGCCATAACGGCGGCGGCAATTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATAATTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCTACCGGCGGCaaa                                                                                                     >  1:3034523/1‑150 (MQ=255)
                                                        ttGGTGCGGTTTTGTTCGCGCGTGAGATCGGAAAGCAGCGCCATAACGGCGGCGGCAATTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATAATTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCTACCGGCGGCAAACACCGcc                                                                                              <  2:2291301/150‑1 (MQ=255)
                                                         tGGTGCGGTTTTGTTCGCGCGTGAGATCGGAAAGCAGCGCCATAACGGCGGCGGCAATTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATCATTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCTACCGGCGGCAAACACCGCCa                                                                                             <  2:907072/150‑1 (MQ=255)
                                                          ggTGCGGTTTTGTTCGCGCGTGAGATCGGAAAGCAGCGCCATAACGGCGGCGGCAATTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATCATTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCTACCGGCGGCAAACACCGCCAg                                                                                            <  2:936033/150‑1 (MQ=255)
                                                          ggTGCGGTTTTGTTCGCGCGTGAGATCGGAAAGCAGCGCCATAACGGCGGCGGCAATTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATAATTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCTACCGGCGGCAAACACCGCCAg                                                                                            <  1:1006676/150‑1 (MQ=255)
                                                          ggTGCGGTTTTGTTCGCGCGTGAGATCGGAAAGCAGCGCCATAACGGCGGCGGCAATTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATAATTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCTACCGGCGGCAAACACCGCCAg                                                                                            >  2:3618054/1‑150 (MQ=255)
                                                                gTTTTGTTCGCGCGTGTGATCGGAAAGCCGCGCCATACCGGCGGCGGCAATTGCTCCAGCGCCGTGTCGCGCCCGGCCCCGAATAATGCCCCAGATGGAGTCGGAAAGCGCCGCGATGACGCTACCGGCGGCCACCTCCGCGAGGCcccc                                                                                      >  2:3951449/1‑150 (MQ=255)
                                                                gTTTTGTTCGCGCGTGAGATCGGAAAGCAGCGCCATAACGGCGGCGGCAATTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATAATTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCTACCGGCGGCAAACACCGCCAGCCCAcc                                                                                      <  2:2663406/150‑1 (MQ=255)
                                                                 ttttGTTCGCGCGTGAGATCGGAAAGCAGCGCCATAACGGCGGCGGCAATTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATAATTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCTACCGGCGGCAAACACCGCCAGCCCACCg                                                                                     <  1:5131889/150‑1 (MQ=255)
                                                                  tttGTTCGCGCGTGAGATCGGAAAGCAGCGCCATAACGGCGGCGGCAATTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATAATTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCTACCGGCGGCAAACACCGCCAGCCCACCGa                                                                                    <  2:4117335/150‑1 (MQ=255)
                                                                  tttGTTCGCGCGTGAGATCGGAAAGCAGCGCCATAACGGCGGCGGCAATTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATAATTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCTACCGGCGGCAAACACCGCCAGCCCACCGa                                                                                    >  2:4021548/1‑150 (MQ=255)
                                                                     gTTCGCGCGTGAGATCGGAAAGCAGCGCCATAACGGCGGCGGCAATTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATAATTCCCCAGATGGAGTCGGAAAGCGCAGCGATAAAGCTACCGGCGGCAAACACCGCCAGCCCACCGACaa                                                                                 >  1:4028573/1‑150 (MQ=255)
                                                                       tCGCGCGTGAGATCGGAAAGCAGCGCCATAACGGCGGCGGCAATTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATCATTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCTACCGGCGGCAAACACCGCCAGCCCACCGACAAtt                                                                               <  1:3896787/150‑1 (MQ=255)
                                                                           gcgTGAGATCGGAAAGCAGCGCCATAACGGCGGCGGCAATTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATAATTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCTACCGGCGGCAAACACCGCCAGCCCACCGACAATTAATg                                                                           <  1:4627407/150‑1 (MQ=255)
                                                                              tGAGATCGGAAAGCAGCGCCATAACGGCGGCGGCAATTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATAATTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCTACCGGCGGCAAACACCGCCAGCCCACCGACAATTAATGGtt                                                                        <  1:749117/150‑1 (MQ=255)
                                                                               gagaTCGGAAAGCAGCGCCATAACGGCGGCGGCAATTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATAATTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCTACCGGCGGCAAACACCGCCAGCCCACCGACAATTAATGGttt                                                                       <  2:3986194/150‑1 (MQ=255)
                                                                                agaTCGGAAAGCAGCGCCATAACGGCGGCGGCAATTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATAATTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCTACCGGCGGCAAACACCGCCAGCCCACCGACAATTAATGGTTTg                                                                      <  1:5024396/150‑1 (MQ=255)
                                                                                 gaTCGGAAAGCAGCGCCATAACGGCGGCGGCAATTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATAATTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCTACCGGCGGCAAACACCGCCAGCCCACCGACAATTAATGGTTTGc                                                                     <  2:1159706/150‑1 (MQ=255)
                                                                                   tCGGAAAGCAGCGCCATAACGGCGGCGGCAATTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATAATTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCTACCGGCGGCAAACACCGCCAGCCCACCGACAATTAATGGTTTGCGa                                                                   <  1:4852618/150‑1 (MQ=255)
                                                                                        aaGCAGCGCCATAACGGCGGCGGCAATTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATCATTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCTACCGGCGGCAAACACCGCCAGCCCACCGACAATTAATGGTTTGCGACCAAt                                                              >  2:4339717/1‑150 (MQ=255)
                                                                                           cagcGCCATAACGGCGGCGGCAATTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATAATTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCTACCGGCGGCAAACACCGCCAGCCCACCGACAATTAATGGTTTGCGACCAATGCg                                                           <  2:4643867/150‑1 (MQ=255)
                                                                                              cgcCATAACGGCGGCGGCAATTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATCATTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCTACCGGCGGCAAACACCGCCAGCCCACCGACAATTAATGGTTTGCGACCAATGCGGTc                                                        >  1:943667/1‑150 (MQ=255)
                                                                                              cgcCATAACGGCGGCGGCAATTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATAATTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCTACCGGCGGCAAACACCGCCAGCCCACCGACAATTAATGGTTTGCGACCAATGCGGTc                                                        >  1:2300704/1‑150 (MQ=255)
                                                                                               gcCATAACGGCGGCGGCAATTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGACTAATTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCTACCGGCGGCAAACACCGCCAGCCCACCGACAATTAATGGGTTGCGACCAATGCGGGCg                                                       >  2:84007/1‑150 (MQ=255)
                                                                                               gcCATAACGGCGGCGGCAATTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATAATTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCTACCGGCGGCAAACACCGCCAGCCCACCGACAATTAATGGTTTGCGACCAATGCGGTCg                                                       <  1:3375426/150‑1 (MQ=255)
                                                                                                  aTAACGGCGGCGGCAATTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATAATTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCTACCGGCGGCAAACACCGCCAGCCCACCGACAATTAATGGTTTGCGACCAATGCGGTCGGaa                                                    <  2:4502773/150‑1 (MQ=255)
                                                                                                  aTAACGGCGGCGGCAATTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATAATTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCTACCGGCGGCAAACACCGCCAGCCCACCGACAATTAATGGTTTGCGACCAATGCGGTCGGaa                                                    <  1:401486/150‑1 (MQ=255)
                                                                                                    aaaggcggcggaAATAGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATAATTCCCCAGATGGAGTCGGAAAGCGCAGAGATACCGCTACCGGCGGCACACACCGACAGCCCACCGACAATTAATGGTTTGCGACAAATGCGGTCGGAAAg                                                  >  1:3031113/4‑150 (MQ=255)
                                                                                                    aaCTGCGGCGGCAATTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATAATTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCTACCGGCGGCAAACACCGCCAGCCCACCGACAATTAATGGTTTGCGACCAATGCGGTCGGAAAg                                                  >  2:1917832/1‑150 (MQ=255)
                                                                                                    aaCGGCGGCGGCAATTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATAATTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCTACCGGCGGCAAACACCGCCAGCCCACCGACAATTAATGGTTTGCGACCAATGCGGTCGGAAAg                                                  >  2:1547240/1‑150 (MQ=255)
                                                                                                    aaCGGCGGCGGCAATTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATAATTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCTACCGGCGGCAAACACCGCCAGCCCACCGACAATTAATGGTTTGCGACCAATGCGGTCGGAAAg                                                  >  2:1369383/1‑150 (MQ=255)
                                                                                                      cggcggcggCAATTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATAATTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCTACCGGCGGCAAACACCGCCAGCCCACCGACAATTAATGGTTTGCGACCAATGCGGTCGGAAAGCa                                                >  1:3224275/1‑150 (MQ=255)
                                                                                                       ggcggcggcAATTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATAATTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCTACCGGCGGCAAACACCGCCAGCCCACCGACAATTAATGGTTTGCGACCAATGCGGTCGGAAAGCAg                                               <  1:4021547/150‑1 (MQ=255)
                                                                                                       ggcggcggcAATTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATAATTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCTACCGGCGGCAAACACCGCCAGCCCACCGACAATTAATGGTTTGCGACCAATGCGGTCGGAAAGCAg                                               >  1:4784725/1‑150 (MQ=255)
                                                                                                         cggcggcAATTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATCATTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCTACCGGCGGCAAACACCGCCAGCCCACCGACAATTAATGGTTTGCGACCAATGCGGTCGGAAAGCAGGc                                             >  2:4094414/1‑150 (MQ=255)
                                                                                                              gcAATTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATAATTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCTACCGGCGGCAAACACCGCCAGCCCACCGACAATTAATGGTTTGCGACCAATGCGGTCGGAAAGCAGGCCAAAc                                        <  2:170490/150‑1 (MQ=255)
                                                                                                                aaTTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATAATTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCTACCGGCGGCAAACACCGCCAGCCCACCGACAATTAATGGTTTGCGACCAATGCGGTCGGAAAGCAGGCCAAACgg                                      <  1:2627070/150‑1 (MQ=255)
                                                                                                                aaTTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATAATTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCTACCGGCGGCAAACACCGCCAGCCCACCGACAATTAATGGTTTGCGACCAATGCGGTCGGAAAGCAGGCCAAACgg                                      >  2:3667107/1‑150 (MQ=255)
                                                                                                                 aTTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATAATTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCTACCGGCGGCAAACACCGCCAGCCCACCGACAATTAATGGTTTGCGACCAATGCGGTCGGAAAGCAGGCCAAACGGa                                     >  1:3496940/1‑150 (MQ=255)
                                                                                                                    gCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATAATTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCTACCGGCGGCAAACACCGCCAGCCCACCGACAATTAATGGTTTGCGACCAATGCGGTCGGAAAGCAGGCCAAACGGAATc                                  <  1:1435856/150‑1 (MQ=255)
                                                                                                                    gCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATAATTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCTACCGGCGGCAAACACCGCCAGCCCACCGACAATTAATGGTTTGCGACCAATGCGGTCGGAAAGCAGGCCAAACGGAATc                                  <  1:820113/150‑1 (MQ=255)
                                                                                                                      tCCAGAGCCTTGTAGCGCCCGGCCCAGAATAATTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCTACCGGCGGCAAACACCGCCAGCCCACCGACAATTAATGGTTTGCGACCAATGCGGTCGGAAAGCAGGCCAAACGGAATCTg                                >  1:4140303/1‑150 (MQ=255)
                                                                                                                       ccAGAGCCTTGTAGCGCCCGGCCCAGAATCATTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCTACCGGCGGCAAACACCGCCAGCCCACCGACAATTAATGGTTTGCGACCAATGCGGTCGGAAAGCAGGCCAAACGGAATCTGa                               >  1:614233/1‑150 (MQ=255)
                                                                                                                         agagCCTTGTAGCGCCCGGCCCAGAATCATTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCTACCGGCGGCAAACACCGCCAGCCCACCGACAATTAATGGTTTGCGACCAATGCGGTCGGAAAGCAGGCCAAACGGAATCTGaaa                             >  1:1558214/1‑150 (MQ=255)
                                                                                                                         agagCCTTGTAGCGCCCGGCCCAGAATAATTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCTACCGGCGGCAAACACCGCCAGCCCACCGACAATTAATGGTTTGCGACCAATGCGGTCGGAAAGCAGGCCAAACGGAATCTGaaa                             >  2:4610077/1‑150 (MQ=255)
                                                                                                                          gagCCTTGTAGCGCCCGGCCCAGAATAATTCACCAGATGGAGTCGGAAATCGAAGCGATAACTCTAAAGGAGGCAAACAACGCAATACAACCGACAATTAATGGTTTGAGACCAATTCGGTCGGAAATCAGGACAAACGGAATCTGaaaa                            >  2:1256977/1‑150 (MQ=255)
                                                                                                                           agCCTTGTAGCGCCCGGCCCAGAATAATTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCTACCGGCGGCAAACACCGCCAGCCCACCGACAATTAATGGTTTGCGACCAATGCGGTCGGAAAGCAGGCCAAACGGAATCTGaaaaa                           >  1:329264/1‑150 (MQ=255)
                                                                                                                            gCCTTGTAGCGCCCGGCCCAGAATAATTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCTACCGGCGGCAAACACCGCCAGCCCACCGACAATTAATGGTTTGCGACCAATGCGGTCGGAAAGCAGGCCAAACGGAATCTGAAAAAc                          <  1:4321495/150‑1 (MQ=255)
                                                                                                                                 gTAGCGCCCGGCCCAGAATAATTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCTACCGGCGGCAAACACCGCCAGCCCACCGACAATTAATGGTTTGCGACCAATGCGGTCGGAAAGCAGGCCAAACGGAATCTGAAAAACGGCCt                     <  1:4534133/150‑1 (MQ=255)
                                                                                                                                  tAGCGCCCGGCCCAGAATAATTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCTACCGGCGGCAAACACCGCCAGCCCACCGACAATTAATGGTTTGCGACCAATGCGGTCGGAAAGCAGGCCAAACGGAATCTGAAAAACGGCCTg                    <  1:2591426/150‑1 (MQ=255)
                                                                                                                                  tAGCGCCCGGCCCAGAATAATTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCTACCGGCGGCAAACACCGCCAGCCCACCGACAATTAATGGTTTGCGACCAATGCGGTCGGAAAGCAGGCCAAACGGAATCTGAAAAACGGCCTg                    <  1:4219558/150‑1 (MQ=255)
                                                                                                                                    gcgcCCGGCCCAGAATAATTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCTACCGGCGGCAAACACCGCCAGCCCACCGACAATTAATGGTTTGCGACCAATGCGGTCGGAAAGCAGGCCAAACGGAATCTGAAAAACGGCCTGAg                  <  1:4925814/150‑1 (MQ=255)
                                                                                                                                        ccGGCCCAGAATAATTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCTACCGGCGGCAAACACCGCCAGCCCACCGACAATTAATGGTTTGCGACCAATGCGGTCGGAAAGCAGGCCAAACGGAATCTGAAAAACGGCCTGAGTCAg              <  1:4150206/150‑1 (MQ=255)
                                                                                                                                         cGGCCCAGAATAATTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCTACCGGCGGCAAACACCGCCAGCCCACCGACAATTAATGGTTTGCGACCAATGCGGTCGGAAAGCAGGCCAAACGGAATCTGAAAAACGGCCTGAGTCAGa             <  2:3148587/150‑1 (MQ=255)
                                                                                                                                           gCCCAGAATAATTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCTACCGGCGGCAAACACCGCCAGCCCACCGACAATTAATGGTTTGCGACCAATGCGGTCGGAAAGCAGGCCAAACGGAATCTGAAAAACGGCCTGAGTCAGAcc           <  2:3319255/150‑1 (MQ=255)
                                                                                                                                             ccAGAATAATTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCTACCGGCGGCAAACACCGCCAGCCCACCGACAATTAATGGTTTGCGACCAATGCGGTCGGAAAGCAGGCCAAACGGAATCTGAAAAACGGCCTGAGTCAGACCAt         <  1:3582875/150‑1 (MQ=255)
                                                                                                                                               aGAATAATTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCTACCGGCGGCAAACACCGCCAGCCCACCGACAATTAATGGTTTGCGACCAATGCGGTCGGAAAGCAGGCCAAACGGAATCTGAAAAACGGCCTGAGTCAGACCATaa       <  1:1925857/150‑1 (MQ=255)
                                                                                                                                               aGAATAATTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCTACCGGCGGCAAACACCGCCAGCCCACCGACAATTAATGGTTTGCGACCAATGCGGTCGGAAAGCAGGCCAAACGGAATCTGAAAAACGGCCTGAGTCAGACCATaa       <  1:3725807/150‑1 (MQ=255)
                                                                                                                                                 aataatTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCTACCGGCGGCAAACACCGCCAGCCCACCGACAATTAATGGTTTGCGACCAATGCGGTCGGAAAGCAGGCCAAACGGAATCTGAAAAACGGCCTGAGTCAGACCATAAAt     >  2:2464115/1‑150 (MQ=255)
                                                                                                                                                    aatTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCTACCGGCGGCAAACACCGCCAGCCCACCGACAATTAATGGTTTGCGACCAATGCGGTCGGAAAGCAGGCCAAACGGAATCTGAAAAACGGCCTGAGTCAGACCATAAATAcc  <  2:1118159/150‑1 (MQ=255)
                                                                                                                                                    aatTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCTACCGGCGGCAAACACCGCCAGCCCACCGACAATTAATGGTTTGCGACCAATGCGGTCGGAAAGCAGGCCAAACGGAATCTGAAAAACGGCCTGAGTCAGACCATAAATAcc  >  2:2113401/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
GCACCATCGCAATGGCAAAGGTAATGCCAAAGCTCACGCCGATAAACGCCATCGCTTTGGTGCGGTTTTGTTCGCGCGTGAGATCGGAAAGCAGCGCCATAACGGCGGCGGCAATTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATAATTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCTACCGGCGGCAAACACCGCCAGCCCACCGACAATTAATGGTTTGCGACCAATGCGGTCGGAAAGCAGGCCAAACGGAATCTGAAAAACGGCCTGAGTCAGACCATAAATACC  >  CP009273/441663‑441960

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑