Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 456,298 T→A 93.8% L652Q (CTG→CAG)  lon → DNA‑binding ATP‑dependent protease La

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273456,2980TA93.8% 939.5 / 40.7 273L652Q (CTG→CAG) lonDNA‑binding ATP‑dependent protease La
Reads supporting (aligned to +/- strand):  ref base T (14/3);  new base A (133/123);  total (147/126)
Fisher's exact test for biased strand distribution p-value = 2.14e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.88e-01

Only 100 of 273 total aligned reads displayed.

TGGACGGAAGTGGGCGGTGACTTGCTGACCATTGAAACCGCATGTGTTCCGGGTAAAGGCAAACTGACCTATACCGGTTCGCTCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACTGGGGATCAACCCTGATTTTTACGAAAAACGTGACATCCACGTCCACGTACCGGAAGGTGCGACGCCGAAAGATGGTCCGAGTGCCGGTATTGCTATGTGCACCGCGCTGGTTTCTTGCCTGACCGGTAACCCGGTTCGTGCCGA  >  CP009273/456150‑456442
                                                                                                                                                    |                                                                                                                                                
 ggACGGAAGTGGGCGGTGACTTGCTGACCATTGAAACCGCATGTGTTCCGGGTAAAGGCAAACTGACCTATACCGGTTCGCTCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAgg                                                                                                                                                <  2:1124378/150‑1 (MQ=255)
    cGGAAGTGGGCGGTGACTTGCTGACCATTGAAACCGCATGTGTTCCGGGTAAAGGCAAACTGACCTATACCGGTTCGCTCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGa                                                                                                                                             >  1:4508744/1‑150 (MQ=255)
    cGGAAGTGGGCGGTGACTTGCTGACCATTGAAACCGCATGTGTTCCGGGTAAAGGCAAACTGACCTATACCGGTTCGCTCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGa                                                                                                                                             <  1:4095353/150‑1 (MQ=255)
     ggAAGTGGGCGGTGACTTGCTGACCATTGAAACCGCATGTGTTCCGGGTAAAGGCAAACTGACCTATACCGGTTCGCTCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGAt                                                                                                                                            >  1:3863894/1‑150 (MQ=255)
       aaGTGGGCGGTGACTTGCTGACCATTGAAACCGCATGTGTTCCGGGTAAAGGCAAACTGACCTATACCGGTTCGCTCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCa                                                                                                                                          <  1:3890337/150‑1 (MQ=255)
        aGTGGGCGGTGACTTGCTGACCATTGAAACCGCATGTGTTCCGGGTAAAGGCAAACTGACCTATACCGGTTCGCTCGGCGAAGTGATGCAGGTGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCaa                                                                                                                                         <  1:674053/150‑1 (MQ=255)
          cgggCGGTGACTTGCTGACCATTGAAACCGCATGTGTTCCGGGTAAAGGCAAACTGACCTATACCGGTTCGCTCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCAAcc                                                                                                                                       <  1:5065910/149‑1 (MQ=255)
                  gACTTGCTGACCATTGAAACCGCATGTGTTCCGGGTAAAGGCAAACTGACCTATACCGGTTCGCTCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGAtttt                                                                                                                               <  2:2752106/150‑1 (MQ=255)
                  gACTTGCTGACCATTGAAACCGCATGTGTTCCGGGTAAAGGCAAACTGACCTATACCGGTTCGCTCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGAtttt                                                                                                                               <  2:286279/150‑1 (MQ=255)
                  gACTTGCTGACCATTGAAACCGCATGTGTTCCGGGTAAAGGCAAACTGACCTATACCGGTTCGCTCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGAtttt                                                                                                                               >  2:3028268/1‑150 (MQ=255)
                   aCTTGCTGACCATTGAAACCGCATGTGTTCCGGGTAAAGGCAAACTGACCTATACCGGTTCGCTCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGAttttt                                                                                                                              <  1:141634/150‑1 (MQ=255)
                     ttGCTGACCATTGAAACCGCATGTGTTCCGGGTAAAGGCAAACTGACCTATACCGGTTCGCTCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGATTTTTAc                                                                                                                            <  1:1671534/150‑1 (MQ=255)
                     ttGCTGACCATTGAAACCGCATGTGTTCCGGGTAAAGGCAAACTGACCTATACCGGTTCGCTCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGATTTTTAc                                                                                                                            <  2:3307258/150‑1 (MQ=255)
                           aCCATTGAAACCGCATGTGTTCCGGGTAAAGGCAAACTGACCTATACCGGTTCGCTCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGATTTTTACGaaaaa                                                                                                                      >  2:1693703/1‑150 (MQ=255)
                               ttGAAACCGCATGTGTTCCGGGTAAAGGCAAACTGACCTATACCGGTTCGCTCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGATTTTTACGAAAAACGTg                                                                                                                  >  1:1091160/1‑150 (MQ=255)
                                tGAAACCGCATGTGTTCCGGGTAAAGGCAAACTGACCTATACCGGTTCGCTCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGATTTTTACGAAAAACGTGa                                                                                                                 >  1:1629152/1‑150 (MQ=255)
                                tGAAACCGCATGTGTTCCGGGTAAAGGCAAACTGACCTATACCGGTTCGCTCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGATTTTTACGAAAAACGTGa                                                                                                                 >  2:2505160/1‑150 (MQ=255)
                                 gAAACCGCATGTGTTCCGGGTAAAGGCAAACTGACCTATACCGGTTCGCTCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGATTTTTACGAAAAACGTGAc                                                                                                                <  1:2157283/150‑1 (MQ=255)
                                  aaaCCGCATGTGTTCCGGGTAAAGGCAAACTGACCTATACCGGTTCGCTCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGATTTTTACGAAAAACGTGACa                                                                                                               <  1:3266193/150‑1 (MQ=255)
                                   aaCCGCATGTGTTCCGGGTAAAGGCAAACTGACCTATACCGGTTCGCTCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGATTTTTACGAAAAACGTGACAt                                                                                                              >  1:963760/1‑150 (MQ=255)
                                   aaCCGCATGTGTTCCGGGTAAAGGCAAACTGACCTATACCGGTTCGCTCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGGTCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGATTTTTACGAAAAACGTGACAt                                                                                                              <  2:1419673/150‑1 (MQ=255)
                                     ccGCATGTGTTCCGGGTAAAGGCAAACTGACCTATACCGGTTCGCTCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGATTTTTACGAAAAACGTGACAtcc                                                                                                            >  2:654574/1‑150 (MQ=255)
                                     ccGCATGTGTTCCGGGTAAAGGCAAACTGACCTATACCGGTTCGCTCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGATTTTTACGAAAAACGTGACAtcc                                                                                                            <  2:497803/150‑1 (MQ=255)
                                       gCATGTGTTCCGGGTAAAGGCAAACTGACCTATACCGGTTCGCTCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACTGGGGATCAACCCTGATTTTTACGAAAAACGTGACAtccac                                                                                                          >  1:3900128/1‑150 (MQ=255)
                                       gCATGTGTTCCGGGTAAAGGCAAACTGACCTATACCGGTTCGCTCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGATTTTTACGAAAAACGTGACAtccac                                                                                                          <  1:3996439/150‑1 (MQ=255)
                                        cATGTGTTCCGGGTAAAGGCAAACTGACCTATACCGGTTCGCTCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGATTTTTACGAAAAACGTGACAtccacg                                                                                                         <  2:4376664/150‑1 (MQ=255)
                                             gtTCCGGGTAAAGGCAAACTGACCTATACCGGTTCGCTCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGATTTTTACGAAAAACGTGACAtccacgtccac                                                                                                    >  1:4350341/1‑150 (MQ=255)
                                             gtTCCGGGTAAAGGCAAACTGACCTATACCGGTTCGCTCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGATTTTTACGAAAAACGTGACAtccacgtccac                                                                                                    <  1:2914757/150‑1 (MQ=255)
                                                 cGGGTAAAGGCAAACTGACCTATACCGGTTCGCTCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGATTTTTACGAAAAACGTGACATCCACGTCCACGTAc                                                                                                >  2:2287121/1‑150 (MQ=255)
                                                  gggTAAAGGCAAACTGACCTATACCGGTTCGCTCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGATTTTTACGAAAAACGTGACATCCACGTCCACGTAcc                                                                                               >  1:376189/1‑150 (MQ=255)
                                                    gTAAAGGCAAACTGACCTATACCGGTTCGCTCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGATTTTTACGAAAAACGTGACATCCACGTCCACGTACCgg                                                                                             >  1:522768/1‑150 (MQ=255)
                                                    gTAAAGGCAAACTGACCTATACCGGTTCGCTCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGATTTTTACGAAAAACGTGACATCCACGTCCACGTACCgg                                                                                             >  1:4569711/1‑150 (MQ=255)
                                                      aaaGGCAAACTGACCTATACCGGTTCGCTCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCTCGTGCGGAAAAAAAGGGGATCAACCCACATTTTTACGAAAAACGTGACATCCACGTCCACGAACAGGaa                                                                                           >  1:1537246/1‑150 (MQ=255)
                                                      aaaGGCAAACTGACCTATACCGGTTCGCTCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGATTTTTACGAAAAACGTGACATCCACGTCCACGTACCGGaa                                                                                           >  1:744639/1‑150 (MQ=255)
                                                       aaGGCAAACTGACCTATACCGGTTCGCTCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGATTTTTACGAAAAACGTGACATCCACGTCCACGTACCGGAAg                                                                                          >  2:392258/1‑150 (MQ=255)
                                                        aGGCAAACTGACCTATACCGGTTCGCTCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGATTTTTACGAAAAACGTGACATCCACGTCCACGTACCGGAAgg                                                                                         >  1:349333/1‑150 (MQ=255)
                                                          gCAAACTGACCTATACCGGTTCGCTCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGATTTTTACGAAAAACGTGACATCCACGTCCACGTACCGGAAGGTg                                                                                       <  1:513369/150‑1 (MQ=255)
                                                            aaaCTGACCTATACCGGTTCGCTCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGATTTTTACGAAAAACGTGACATCCACGTCCACGTACCGGAAGGTGCg                                                                                     >  2:1750529/1‑150 (MQ=255)
                                                            aaaCTGACCTATACCGGTTCGCTCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGATTTTTACGAAAAACGTGACATCCACGTCCACGTACCGGAAGGTGCg                                                                                     <  2:1931958/150‑1 (MQ=255)
                                                            aaaCTGACCTATACCGGTTCGCTCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGATTTTTACGAAAAACGTGACATCCACGTCCACGTACCGGAAGGTGCg                                                                                     <  2:3002841/150‑1 (MQ=255)
                                                              aCTGACCTATACCGGTTCGCTCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGATTTTTACGAAAAACGTGACATCCACGTCCACGTACCGGAAGGTGCGAc                                                                                   <  2:376103/150‑1 (MQ=255)
                                                               cTGACCTATACCGGTTCGCTCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGATTTTTACGAAAAACGTGACATCCACGTCCACGTACCGGAAGGTGCGACg                                                                                  >  1:4062212/1‑150 (MQ=255)
                                                                tGACCTATACCGGTTCGCTCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGATTTTTACGAAAAACGTGACATCCACGTCCACGTACCGGAAGGTGCGACGc                                                                                 >  2:707894/1‑150 (MQ=255)
                                                                 gACCTATACCGGTTCGCTCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGATTTTTACGAAAAACGTGACATCCACGTCCACGTACCGGAAGGTGCGACGcc                                                                                <  2:3634255/150‑1 (MQ=255)
                                                                  aCCTAGACCGGTTCGCTCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGATTTTTACGAAAAACGTGACATCCACGTCCACGTACCGGAAGGTGCGACGCCg                                                                               <  2:5072057/150‑1 (MQ=255)
                                                                    cTATACCGGTTCGCTCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACTGGGGATCAACCCTGATTTTTACGAAAAACGTGACATCCACGTCCACGTACCGGAAGGTGCGACGCCGaa                                                                             >  2:239552/1‑150 (MQ=255)
                                                                      ataCCGGTTCGCTCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGATTTTTACGAAAAACGTGACATCCACGTCCACGTACCGGAAGGTGCGACGCCGAAAg                                                                           >  1:232950/1‑150 (MQ=255)
                                                                       taCCGGTTCGCTCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGATTTTTACGAAAAACGTGACATCCACGTCCACGTACCGGAAGGTGCGACGCCGAAAGa                                                                          >  2:401190/1‑150 (MQ=255)
                                                                       taCCGGTTCGCTCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGATTTTTACGAAAAACGTGACATCCACGTCCACGTACCGGAAGGTGCGACGCCGAAAGa                                                                          >  2:400823/1‑150 (MQ=255)
                                                                          cGGTTCGCTCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGATTTTTACGAAAAACGTGACATCCACGTCCACGTACCGGAAGGTGCGACGCCGAAAGATgg                                                                       <  2:622759/150‑1 (MQ=255)
                                                                           ggTTCGCTCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGATTTTTACGAAAAACGTGACATCCACGTCCACGTACCGGAAGGTGCGACGCCGAAAGATGGt                                                                      <  1:710953/150‑1 (MQ=255)
                                                                           ggTTCGCTCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGATTTTTACGAAAAACGTGACATCCACGTCCACGTACCGGAAGGTGCGACGCCGAAAGATGGt                                                                      >  1:137374/1‑150 (MQ=255)
                                                                            gTTCGCTCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGATTTTTACGAAAAACGTGACATCCACGTCCACGTACCGGAAGGTGCGACGCCGAAAGATGGTc                                                                     >  1:3825250/1‑150 (MQ=255)
                                                                               cGCTCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGATTTTTACGAAAAACGTGACATCCACGTCCACGTACCGGAAGGTGCGACGCCGAAAGATGGTCCGa                                                                  <  2:1225383/150‑1 (MQ=255)
                                                                               cGCTCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGATTTTTACGAAAAACGTGACATCCACGTCCACGTACCGGAAGGTGCGACGCCGAAAGATGGTCCGa                                                                  >  2:1562050/1‑150 (MQ=255)
                                                                                gCTCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGATTTTTACGAAAAACGTGACATCCACGTCCACGTACCGGAAGGTGCGACGCCGAAAGATGGTCCGAg                                                                 <  1:621336/150‑1 (MQ=255)
                                                                                     gCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGATTTTTACGAAAAACGTGACATCCACGTCCACGTACCGGAAGGTGCGACGCCGAAAGATGGTCCGAGTGCCg                                                            <  2:678170/150‑1 (MQ=255)
                                                                                      cGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGATTTTTACGAAAAACGTGACATCCACGTCCACGTACCGGAAGGTGCGACGCCGAAAGATGGTCCGAGTGCCgg                                                           <  2:3101981/150‑1 (MQ=255)
                                                                                       gAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGATTTTTACGAAAAACGTGACATCCACGTCCACGTACCGGAAGGTGCGACGCCGAAAGATGGTCCGAGTGCCGGt                                                          >  1:2493213/1‑150 (MQ=255)
                                                                                         aGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGATTTTTACGAAAAACGTGACATCCACGTCCACGTACCGGAAGGTGCGACGCCGAAAGATGGTCCGAGTGCCGGTAt                                                        >  1:1115418/1‑150 (MQ=255)
                                                                                         aGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGATTTTTACGAAAAACGTGACATCCACGTCCACGTACCGGAAGGTGCGACGCCGAAAGATGGTCCGAGTGCCGGTAt                                                        <  2:4320904/150‑1 (MQ=255)
                                                                                           tGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGATTTTTACGAAAAACGTGACATCCACGTCCACGTACCGGAAGGTGCGACGCCGAAAGATGGTCCGAGTGCCGGTATTg                                                      >  2:3135861/1‑150 (MQ=255)
                                                                                               gCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGATTTTTACGAAAAACGTGACATCCACGTCCACGTACCGGAAGGTGCGACGCCGAAAGATGGTCCGAGTGCCGGTATTGCTAt                                                  <  1:4370700/150‑1 (MQ=255)
                                                                                                 aGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGATTTTTACGAAAAACGTGACATCCACGTCCACGTACCGGAAGGTGCGACGCCGAAAGATGGTCCGAGTGCCGGTATTGCTAtgt                                                <  1:2054815/150‑1 (MQ=255)
                                                                                                 aGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGATTTTTACGAAAAACGTGACATCCACGTCCACGTACCGGAAGGTGCGACGCCGAAAGATGGTCCGAGTGCCGGTATTGCTAtgt                                                >  1:3006662/1‑150 (MQ=255)
                                                                                                 aGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGATTTTTACGAAAAACGTGACATCCACGTCCACGTACCGGAAGGTGCGACGCCGAAAGATGGTCCGAGTGCCGGTATTGCTAtgt                                                >  1:360324/1‑150 (MQ=255)
                                                                                                  ggAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACTGGGGATCAACCCTGATTTTTACGAAAAACGTGACATCCACGTCCACGTACCGGAAGGTGCGACGCCGAAAGATGGTCCGAGTGCCGGTATTGCTAtgtg                                               >  1:2283303/1‑150 (MQ=255)
                                                                                                  ggAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGATTTTTACGAAAAACGTGACATCCACGTCCACGTACCGGAAGGTGCGACGCCGAAAGATGGTCCGAGTGCCGGTATTGCTAtgtg                                               >  1:4930639/1‑150 (MQ=255)
                                                                                                   gAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGATTTTTACGAAAAACGTGACATCCACGTCCACGTACCGGAAGGTGCGACGCCGAAAGATGGTCCGAGTGCCGGTATTGCTATGTGc                                              >  1:1904488/1‑150 (MQ=255)
                                                                                                    aGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGATTTTTACGAAAAACGTGACATCCACGTCCACGTACCGGAAGGTGCGACGCCGAAAGATGGTCCGAGTGCCGGTATTGCTATGTGCa                                             <  1:5000214/150‑1 (MQ=255)
                                                                                                     gTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGATTTTTACGAAAAACGTGACATCCACGTCCACGTACCGGAAGGTGCGACGCCGAAAGATGGTCCGAGTGCCGGTATTGCTATGTGCAc                                            <  1:1733304/150‑1 (MQ=255)
                                                                                                     gTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGATTTTTACGAAAAACGTGACATCCACGTCCACGTACCGGAAGGTGCGACGCCGAAAGATAGTCCGAGTGCCGGTATTGCTATGTGCAc                                            >  1:1177078/1‑150 (MQ=255)
                                                                                                        cATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGATTTTTACGAAAAACGTGACATCCACGTCCACGTACCGGAAGGTGCGACGCCGAAAGATGGTCCGAGTGCCGGTATTGCTATGTGCACcgc                                         <  2:322067/150‑1 (MQ=255)
                                                                                                          ttCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGATTTTTACGAAAAACGTGACATCCACGTCCACGTACCGGAAGGTGCGACGCCGAAAGATGGTCCGAGTGCCGGTATTGCTATGTGCACCgcgc                                       >  2:4860897/1‑150 (MQ=255)
                                                                                                           tCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGATTTTTACGAAAAACGTGACATCCACGTCCACGTACCGGAAGGTGCGACGCCGAAAGATGGTCCGAGTGCCGGTATTGCTATGTGCACCGCGCt                                      >  2:4428755/1‑150 (MQ=255)
                                                                                                              ggcggcGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGATTTTTACGAAAAACGTGACATCCACGTCCACGTACCGGAAGGTGCGACGCCGAAAGATGGTCCGAGTGCCGGTATTGCTATGTGCACCGCGCTGGt                                   <  2:966205/150‑1 (MQ=255)
                                                                                                                cggcgTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGATTTTTACGAAAAACGTGACATCCACGTCCACGTACCGGAAGGTGCGACGCCGAAAGATGGTCCGAGTGCCGGTATTGCTATGTGCACCGCGCTGGttt                                 >  2:3696400/1‑150 (MQ=255)
                                                                                                                 ggcgTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGATTTTTACGAAAAACGTGACATCCACGTCCACGTACCGGAAGGTGCGACGCCGAAAGATGGTCCGAGTGCCGGTATTGCTATGTGCACCGCGCTGGTTTc                                >  1:4268022/1‑150 (MQ=255)
                                                                                                                   cgTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGATTTTTACGAAAAACGTGACATCCACGTCCACGTACCGGAAGGTGCGACGCCGAAAGATGGTCCGAGTGCCGGTATTGCTATGTGCACCGCGCTGGTTTCtt                              <  1:1991896/150‑1 (MQ=255)
                                                                                                                    gTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGATTTTTACGAAAAACGTGACATCCACGTCCACGTACCGGAAGGTGCGACGCCGAAAGATGGTCCGAGTGCCGGTATTGCTATGTGCACCGCGCTGGTTTCTTg                             >  2:3832673/1‑150 (MQ=255)
                                                                                                                      tAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGATTTTTACGAAAAACGTGACATCCACGTCCACGTACCGGAAGGTGCGACGCCGAAAGATGGTCCGAGTGCCGGTATTGCTATGTGCACCGCGCTGGTTTCTTGcc                           >  2:3539723/1‑150 (MQ=255)
                                                                                                                         cGGTGGTTCGTGCGCGTGCGGAAAATCAGGGGATCAACCCTGATTTTTACGAAAAACGTGACATCCACGTCCACGTACCGGAAGGTGCGACGCCGAAAGATGGTCCGAGTGCCGGTATTGCTATGTGCACCGCGCTGGTTTCTTGCCTGa                        >  2:200359/1‑150 (MQ=255)
                                                                                                                         cGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGATTTTTACGAAAAACGTGACATCCACGTCCACGTACCGGAAGGTGCGACGCCGAAAGATGGTCCGAGTGCCGGTATTGCTATGTGCACCGCGCTGGTTTCTTGCCTGa                        >  1:3731839/1‑150 (MQ=255)
                                                                                                                          ggtggtTCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGATTTTTACGAAAAACGTGACATCCACGTCCACGTACCGGAAGGTGCGACGCCGAAAGATGGTCTGAGTGCCGGTATTGCTATGTGCACCGCGCTGGTTTCTTGCCTGAc                       >  1:3124209/1‑150 (MQ=255)
                                                                                                                             ggtTCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGATTTTTACGAAAAACGTGACATCCACGTCCACGTACCGGAAGGTGCGACGCCGAAAGATGGTCCGAGTGCCGGTATTGCTATGTGCACCGCGCTGGTTTCTTGCCTGACCgg                    <  2:866296/150‑1 (MQ=255)
                                                                                                                              gtTCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGATTTTTACGAAAAACGTGACATCCACGTCCACGTACCGGAAGGTGCGACGCCGAAAGATGGTCCGAGTGCCGGTATTGCTATGTGCACCGCGCTGGTTTCTTGCCTGACCGGt                   >  2:4681844/1‑150 (MQ=255)
                                                                                                                                tCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGATTTTTACGAAAAACGTGACATCCACGTCCACGTACCGGAAGGTGCGACGCCGAAAGATGGTCCGAGTGCCGGTATTGCTATGTGCACCGCGCTGGTTTCTTGCCTGACCGGTaa                 >  2:2162317/1‑150 (MQ=255)
                                                                                                                                 cgtgcgcgtgcgGAAAAACAGGGGATCAACCCTGATTTTTACGAAAAACGTGACATCCACGTCCACGTACCGGAAGGTGCGACGCCGAAAGATGGTCCGAGTGCCGGTATTGCTATGTGCACCGCGCTGGTTTCTTGCCTGACCGGTAAc                >  1:2669318/1‑150 (MQ=255)
                                                                                                                                  gtgcgcgtgcgGAAAAACAGGGGATCAACCCTGATTTTTACGAAAAACGTGACATCCACGTCCACGTACCGGAAGGTGCGACGCCGAAAGATGGTCCGAGTGCCGGTATTGCTATGTGCACCGCGCTGGTTTCTTGCCTGACCGGTAAcc               <  2:76873/150‑1 (MQ=255)
                                                                                                                                  gtgcgcgtgcgGAAAAACAGGGGATCAACCCTGATTTTTACGAAAAACGTGACATCCACGTCCACGTACCGGAAGGTGCGACGCCGAAAGATGGTCCGAGTGCCGGTATTGCTATGTGCACCGCGCTGGTTTCTTGCCTGACCGGTAAcc               >  1:3098767/1‑150 (MQ=255)
                                                                                                                                   tgcgcgtgcgGAAAAACTGGGGATCAACCCTGATTTTTACGAAAAACGTGACATCCACGTCCACGTACCGGAAGGTGCGACGCCGAAAGATGGTCCGAGTGCCGGTATTGCTATGTGCACCGCGCTGGTTTCTTGCCTGACCGGTAAccc              <  1:471137/150‑1 (MQ=255)
                                                                                                                                      gcgtgcgGAAAAACAGGGGATCAACCCTGATTTTTACGAAAAACGTGACATCCACGTCCACGTACCGGAAGGTGCGACGCCGAAAGATGGTCCGAGTGCCGGTATTGCTATGTGCACCGCGCTGGTTTCTTGCCTGACCGGTAACCCGGt           <  2:4683817/150‑1 (MQ=255)
                                                                                                                                       cgtgcgGAAAAACAGGGGATCAACCCTGATTTTTACGAAAAACGTGACATCCACGTCCACGTACCGGAAGGTGCGACGCCGAAAGATGGTCCGAGTGCCGGTATTGCTATGTGCACCGCGCTGGTTTCTTGCCTGACCGGTAACCCGGtt          >  2:3196015/1‑150 (MQ=255)
                                                                                                                                        gtgcgGAAAAACTGGGGATCAACCCTGATTTTTACGAAAAACGTGACATCCACGTCCACGTACCGGAAGGTGCGACGCCGAAAGATGGTCCGAGTGCCGGTATTGCTATGTGCACCGCGCTGGTTTCTTGCCTGACCGGTAACCCGGTTc         >  2:2236142/1‑150 (MQ=255)
                                                                                                                                         tgcgGAAAAACAGGGGATCAACCCTGATTTTTACGAAAAACGTGACATCCACGTCCACGTACCGGAAGGTGCGACGCCGAAAGATGGTCCGAGTGCCGGTATTGCTATGTGCACCGCGCTGGTTTCTTGCCTGACCGGTAACCCGGTTCg        <  1:2874251/150‑1 (MQ=255)
                                                                                                                                          gcgGAAAAACAGGGGATAAACCCTGATTATTACGAAAAACGTGACATCCACGTCCTCGTACCGGAAGGTGCGTCGCCGTAAGATGGTCCGAGTGCCGGTATTGCTATGTGCACCGCGCTGGTTTCTTGCCTGACCGGTAACCCGGTTCGt       <  1:2186342/150‑1 (MQ=255)
                                                                                                                                           cgGAAAAACTGGGGATCAACCCTGATTTTTACGAAAAACGTGACATCCACGTCCACGTACCGGAAGGTGCGACGCCGAAAGATGGTCCGAGTGCCGGTATTGCTATGTGCACCGCGCTGGTTTCTTGCCTGACCGGTAACCCGGTTCGTg      >  1:3145904/1‑150 (MQ=255)
                                                                                                                                            ggAAAAACAGGGGATCAACCCTGATTTTTACGAAAAACGTGACATCCACGTCCACGTACCGGAAGGTGCGACGCCGAAAGATGGTCCGAGTGCCGGTATTGCTATGTGCACCGCGCTGGTTTCTTGCCTGACCGGTAACCCGGTTCGTGc     >  2:418550/1‑150 (MQ=255)
                                                                                                                                             gAAAAACAGGGGATCAACCCTGATTTTTACGAAAAACGTGACATCCACGTCCACGTACCGGAAGGTGCGACGCCGAAAGATGGTCCGAGTGCCGGTATTGCTATGTGCACCGCGCTGGTTTCTTGCCTGACCGGTAACCCGGTTCGTGcc    <  2:1997263/150‑1 (MQ=255)
                                                                                                                                               aaaaCAGGGGATCAACCCTGATTTTTACGAAAAACGTGACATCCACGTCCACGTACCGGAAGGTGCGACGCCGAAAGATGGTCCGAGTGCCGGTATTGCTATGTGCACCGCGCTGGTTTCTTGCCTGACCGGTAACCCGGTTCGTGCCGa  >  2:4084650/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                
TGGACGGAAGTGGGCGGTGACTTGCTGACCATTGAAACCGCATGTGTTCCGGGTAAAGGCAAACTGACCTATACCGGTTCGCTCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACTGGGGATCAACCCTGATTTTTACGAAAAACGTGACATCCACGTCCACGTACCGGAAGGTGCGACGCCGAAAGATGGTCCGAGTGCCGGTATTGCTATGTGCACCGCGCTGGTTTCTTGCCTGACCGGTAACCCGGTTCGTGCCGA  >  CP009273/456150‑456442

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑