Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 469,770 C→A 11.1% R283L (CGT→CTT)  tesB ← acyl‑CoA thioesterase 2

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273469,7700CA11.1% 898.9 / 61.3 258R283L (CGT→CTT) tesBacyl‑CoA thioesterase 2
Reads supporting (aligned to +/- strand):  ref base C (107/122);  new base A (15/14);  total (122/136)
Fisher's exact test for biased strand distribution p-value = 6.94e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 263 total aligned reads displayed.

TCTGACGGTTGGTCTGCGTGTACCGGAAGAGCAGGAGCGAGAAGGGCTGGATGTCAACAGCCACGGCGAGAATGCCTATAACGCGTAACAAGCACTGCAAAAAACAGCCGGACGGTTTTCACCTCCGGCTATTTTTTTAATTGTGATTACGCATCACCCCTTCCTGAACGGTCGAGGCAACCAGTACGCCGTCTTGGGTATAAAACTCACCGCGCACAAAGCCACGTGCGCTGGACGCCGAGGTGCTCTCCACGCTATACAGCAGCCATTCATTCAAATTAAACGGGCGATGGAACCAC  >  CP009273/469621‑469919
                                                                                                                                                     |                                                                                                                                                     
  tGACGGTTGGTCTGCGTGTACCGGAAGAGCAGGAGCGAGAAGGGCTGGATGTCAACAGCCACGGCGAGAATGCCTATAACGCGTAACAAGCACTGCAAAAAACAGCCGGACGGTTTTCACCTCCGGCTATTTTTTTAATTGTGATTACGc                                                                                                                                                     <  1:3144511/150‑1 (MQ=255)
    aCGGTTGGTCTGCGTGTACCGGAAGAGCAGGAGCGAGAAGGGCTGGATGTCAACAGCCACGGCGAGAATGCCTATAACGCGTAACAAGCACTGCAAAAAACAGCCGGACGGTTTTCACCTCCGGCTATTTTTTTAATTGTGATTACGCAt                                                                                                                                                   <  1:4287961/150‑1 (MQ=255)
      ggTTGGTCTGCGTGTACCGGAAGAGCAGGAGCGAGAAGGGCTGGATGTCAACAGCCACGGCGAGAATGCCTATAACGCGTAACAAGCACTGCAAAAAACAGCCGGACGGTTTTCACCTCCGGCTATTTTTTTAATTGTGATTACGCATCa                                                                                                                                                 <  2:640848/150‑1 (MQ=255)
       gTTGGTCTGCGTGTACCGGAAGAGCAGGAGCGAGAAGGGCTGGATGTCAACAGCCACGGCGAGAATGCCTATAACGCGTAACAAGCACTGCAAAAAACAGCCGGACGGTTTTCACCTCCGGCTATTTTTTTAATTGTGATTACGCATCAc                                                                                                                                                >  2:1268841/1‑150 (MQ=255)
        ttGGTCTGCGTGTACCGGAAGAGCAGGAGCGAGAAGGGCTGGATGTCAACAGCCACGGCGAGAATGCCTATAACGCGTAACAAGCACTGCAAAAAACAGCCGGACGGTTTTCACCTCCGGCTATTTTTTTAATTGTGATTACGCATCAcc                                                                                                                                               <  2:38760/150‑1 (MQ=255)
        ttGGTCTGCGTGTACCGGAAGAGCAGGAGCGAGAAGGGCTGGATGTCAACAGCCACGGCGAGAATGCCTATAACGCGTAACAAGCACTGCAAAAAACAGCCGGACGGTTTTCACCTCCGGCTATTTTTTTAATTGTGATTACGCATCAcc                                                                                                                                               <  2:426615/150‑1 (MQ=255)
          ggTCTGCGTGTACCGGAAGAGCAGGAGCGAGAAGGGCTGGATGTCAACAGCCACGGCGAGAATGCCTATAACGCGTAACAAGCACTGCAAAAAACAGCCGGACGGTTTTCACCTCCGGCTATTTTTTTAATTGTGATTAAGCATCAcccc                                                                                                                                             >  2:416609/1‑150 (MQ=255)
            tCTGCGTGTACCGGAAGAGCAGGAGCGAGAAGGGCTGGATGTCAACAGCCACGGCGAGAATGCCTATAACGCGTAACAAGCACTGCAAAAAACAGCCGGACGGTTTTCACCTCCGGCTATTTTTTTAATTGTGATTACGCATCACCCCtt                                                                                                                                           <  1:1626141/150‑1 (MQ=255)
             cTGCGTGTACCGGAAGAGCAGGAGCGAGAAGGGCTGGATGTCAACAGCCACGGCGAGAATGCCTATAACGCGTAACAAGCACTGCAAAAAACAGCCGGACGGTTTTCACCTCCGGCTATTTTTTTAATTGTGATTACGCATCACCCCTTc                                                                                                                                          <  1:481777/150‑1 (MQ=255)
               gCGTGTACCGGAAGAGCAGGAGCGAGAAGGGCTGGATGTCAACAGCCACGGAGAGAATGCCTATAACGCGTAACAAGCACTGCAAAAAACAGCCTGACGGTTTTCACCTCCGGCTATTTTTTTAATTGTGATTACGCATCACCCCTTCCt                                                                                                                                        <  1:929902/150‑1 (MQ=255)
                  tgtACCGGAAGAGCAGGAGCGAGAAGGGCGGGATGTCAACAGCCACGGCGAGAATGCCTATAACGCGTAACAAGCACTGCAAAAAACAGCCGGACGGTTTTCACCTCCGGCTATTTTTTTAATTGTGATTACGCATCACCCCTTCCTGaa                                                                                                                                     <  1:3611980/150‑1 (MQ=255)
                   gtACCGGAAGAGCAGGAGCGAGAAGTGCTGGATGTCAACAGCCACGGCGAGAATGCCTATAACGCGTAACAAGCACTGCAAAAAACAGCCGGACGGTTTTCACCTCCGGCTATTTTATTAATTGTGATTACGCATCACCCCTTCCTGAAc                                                                                                                                    >  1:1707600/1‑150 (MQ=255)
                    tACCGGAAGAGCAGGAGCGAGAAGGGCTGGATGTCAACAGCCACGGCGAGAATGCCTATAACGCGTAACAAGCACTGCAAAAAACAGCCGGACGGTTTTCACCTCCGGCTATTTTTTTAATTGTGATTACGCATCACCCCTTCCTGAACg                                                                                                                                   >  2:533602/1‑150 (MQ=255)
                    tACCGGAAGAGCAGGAGCGAGAAGGGCTGGATGTCAACAGCCACGGCGAGAATGCCTATAACGCGTAACAAGCACTGCAAAAAACAGCCGGACGGTTTTCACCTCCGGCTATTTTTTTAATTGTGATTACGCATCACCCCTTCCTGAACg                                                                                                                                   <  2:3536799/150‑1 (MQ=255)
                    tACCGGAAGAGCAGGAGCGAGAAGGGCTGGATGTCAACAGCCACGGCGAGAATGCCTATAACGCGTAACAAGCACTGCAAAAAACAGCCGGACGGTTTTCACCTCCGGCTATTTTTTTAATTGTGATTACGCATCACCCCTTCCTGAACg                                                                                                                                   <  1:2387273/150‑1 (MQ=255)
                       cGGAAGAGCAGGAGCGAGAAGGGCTGGATGTCAACAGCCACGGCGAGAATGCCTATAACGCGTAACAAGCACTGCAAAAAACAGCCGGACGGTTTTCACCTCCGGCTATTTTTTTAATTGTGATTACGCATCACCCCTTCCTGAACGGTc                                                                                                                                <  2:939737/150‑1 (MQ=255)
                       cGGAAGAGCAGGAGCGAGAAGGGCTGGATGTCAACAGCCACGGCGAGAATGCCTATAACGCGTAACAAGCACTGCAAAAAACAGCCGGACGGTTTTCACCTCCGGCTATTTTTTTAATTGTGATTACGCATCACCCCTTCCTGAACGGTc                                                                                                                                >  2:2963387/1‑150 (MQ=255)
                         gAAGAGCAGGAGCGAGAAGGGCTGGATGTCAACAGCCACGGCGAGACTGCCTATAACGCGTAACAAGCACTGCAAAAAACAGCCGGACGGTTTTCACCTCCGGCTATTTTTTTAATTGTGATTACGCATCACCCCTTCCTGAACGGTCGa                                                                                                                              <  2:4301672/150‑1 (MQ=255)
                               cAGGAGCGAGAAGGGCTGGATGTCAACAGCCACGGCGAGAATGCCTATAACGCGTAACAAGCACTGCAAAAAACAGCCGGACGGTTTTCACCTCCGGCTATTTTTTTAATTGTGATTACGCATCACCCCTTCCTGAACGGTCGAGGCAAc                                                                                                                        <  2:2166729/150‑1 (MQ=255)
                                aGGAGCGAGAAGGGCTGGATGTCAACAGCCACGGCGAGAATGCCTATAACGCGTAACAAGCACTGCAAAAAACAGCCGGACGGTTTTCACCTCCGGCTATTTTTTTAATTGTGATTACGCATCACCCCTTCCTGAACGGTCGAGGCAAcc                                                                                                                       <  2:2484702/150‑1 (MQ=255)
                                aGGAGCGAGAAGGGCTGGATGTCAACAGCCACGGCGAGAATGCCTATAACGCGTAACAAGCACTGCAAAAAACAGCCGGACGGTTTTCACCTCCGGCTATTTTTTTAATTGTGATTACGCATCACCCCTTCCTGAACGGTCGAGGCAAcc                                                                                                                       >  1:1289732/1‑150 (MQ=255)
                                 ggAGCGAGAAGGGCTGGATGTCAACAGCCACGGCGAGAATGCCTCTAACGCGTAACAAGCACTGCAAAAAACAGCCGGACGGTTTTCACCTCCGGCTATTTTTTTAATTGTGATTACGCATCACCCCTTCCTGAACGGTCGAGGCAACCa                                                                                                                      >  2:2882679/1‑150 (MQ=255)
                                 ggAGCGAGAAGGGCTGGATGTCAACAGCCACGGCGAGAATGCCTATAACGCGTAACAAGCACTGCAAAAAACAGCCGGACGGTTTTCACCTCCGGCTATTTTTTTAATTGTGATTACGCATCACCCCTTCCTGAACGGTCGAGGCAACCa                                                                                                                      <  2:2407511/150‑1 (MQ=255)
                                   aGCGAGAAGGGCTGGATGTCAACAGCCACGGCGAGAATGCCTATAACGCGTAACAAGCACTGCAAAAAACAGCCGGACGGTTTTCACCTCCGGCTATTTTTTTAATTGTGATTACGCATCACCCCTTCCTGAACGGTCGAGGCAACCAGt                                                                                                                    >  2:4912372/1‑150 (MQ=255)
                                    gCGAGAAGGGCTGGATGTCAACAGCCACGGCGAGAATGCCTATAACGCGTAACAAGCACTGCAAAAAACAGCCGGACGGTTTTCACCTCCGGCTATTTTTTTAATTGTGATTACGCATCACCCCTTCCTGAACGGTCGAGGCAACCAGTa                                                                                                                   <  2:1874318/150‑1 (MQ=255)
                                      gagaAGGGCTGGAAGTCAACAGCCACGGCGAGAATGCCTATAACGCGTAACAAGCACTGCAAAAAACAGCCGGACGGTTTTCACCTCCGGCTATTTTTTTAATTGTGATTACGCATCACCCCTTCCTGAACGGTCGAGGCACCCAGTACg                                                                                                                 >  2:3714812/1‑150 (MQ=255)
                                               tGGATGTCAACAGCCACGGCGAGAATGCCTATAACGCGTAACAAGCACTGCAAAAAACAGCCGGACGGTTTTCACCTCCGGCTATTTTTTTAATTGTGATTAAGCATCACCCCTTCCTGAACGGTCGAGGCAACCAGTACGCCGTCTTgg                                                                                                        >  1:1845050/1‑150 (MQ=255)
                                                     tCAACAGCCACGGCGAGAATGCCTATAACGCGTAACAAGCACTGCAAAAAACAGCCGGACGGTTTTCACCTCCGGCTATTTTTTTAATTGTGATTACGCATCACCCCTTCCTGAACGGTCGAGGCAACCAGTACGCCGTCTTGGGTATaa                                                                                                  <  1:4020402/150‑1 (MQ=255)
                                                     tCAACAGCCACGGCGAGAATGCCTATAACGCGTAACAAGCACTGCAAAAAACAGCCGGACGGTTCTCACCTCCGGCTATTTTTTTAATTGTGATTACGCATCACCCCTTCCTGAACGGTCGAGGCAACCAGTACGCCGTCTTGGGTATaa                                                                                                  <  1:2492745/150‑1 (MQ=255)
                                                      cAACAGCCACGGCGAGAATGCCTATAACGCGTAACAAGCACTGCAAAAAACAGCCGGACGGTTTTCACCTCCGGCTATTTTTTTAATTGTGATTACGCATCACCCCTTCCTGAACGGTCGAGGCAACCAGTACGCCGTCTTGGGTATaaa                                                                                                 >  2:2954540/1‑150 (MQ=255)
                                                        aCAGCCACGGCGAGAATGCCTATAACGCGTAACAAGCACTGCAAAAAACAGCCGGACGGTTTTCACCTCCGGCTATTTTTTTAATTGTGATTACGCATCACCCCTTCCTGAACGGTCGAGGCAACCAGTACGCCGTCTTGGGTATAAAAc                                                                                               >  2:191071/1‑150 (MQ=255)
                                                         cAGCCACGGCGAGAATGCCTATAACGCGTAACAAGCACTGCAAAAAACAGCCGGACGGTTTTCACCTCCGGCTATTTTTTTAATTGTGATTACGCATCACCCCTTCCTGAACGGTCGAGGCAACCAGTACGCCGTCTTGGGTATAAAACt                                                                                              <  2:1828198/150‑1 (MQ=255)
                                                             cACGGCGAGAATGCCTATAACGCGTAACAAGCACTGCAAAAAACAGCCGGACGGTTTTCACCTCCGGCTATTTTTTTAATTGTGATTACGCATCACCCCTTCCTGAACGGTCGAGGCAACCAGTACGCCGTCTTGGGTATAAAACTCAcc                                                                                          >  1:1011355/1‑150 (MQ=255)
                                                              aCGGCGAGAATGCCTATAACGCGTAACAAGCACTGCAAAAAACAGCCGGACGGTTTTCACCTCCGGCTATTTTTTTAATTGTGATTACGCATCACCCCTTCCTGAACGGTCGAGGCAACCAGTACGCCGTCTTGGGTATAAAACTCACcg                                                                                         <  1:2086566/150‑1 (MQ=255)
                                                               cGGCGAGAATGCCTATAACGCGTAACAAGCACTGCAAAAAACAGCCGGACGGTTTTCACCTCCGGCTATTTTTTTAATTGTGATTACGCATCACCCCTTCCTGAACGGTCGAGGCAACCAGTACGCCGTCTTGGGTATAAAACTCACcgc                                                                                        <  2:4139060/150‑1 (MQ=255)
                                                                 gCGAGAATGCCTATAACGCGTAACAAGCACTGCAAAAAACAGCCGGACGGTTTTCACCTCCGGCTATTTTTTTAATTGTGATTACGCATCACCCCTTCCTGAACGGTCGAGGCAACCAGTACGCCGTCTTGGGTATAAAACTCACCgcgc                                                                                      >  2:5104352/1‑150 (MQ=255)
                                                                  cGAGAATGCCTATAACGCGTAACAAGCACTGCAAAAAACAGCCGGACGGTTTTCACCTCCGGCTATTTTTTTAATTGTGATTACGCATCACCCCTTCCTGAACGGTCGAGGCAACCAGTACGCCGTCTTGGGTCTAAAACTCACCGCGca                                                                                     >  1:4833632/1‑150 (MQ=255)
                                                                  cGAGAATGCCTATAACGCGTAACAAGCACTGCAAAAAACAGCCGGACGGTTTTCACCTCCGGCTATTTTTTTAATTGTGATTACGCATCACCCCTTCCTGAACGGTCGAGGCAACCAGTACGCCGTCTTGGGTATAAAACTCACCGCGca                                                                                     >  1:989451/1‑150 (MQ=255)
                                                                      aaTGCCTATAACGCGTAACAAGCACTGCAAAAAACAGCCGGACGGTTTTCACCTCCGGCTATTTTTTTAATTGTGATTACGCATCACCCCTTCCTGAACGGTCGAGGCAACCAGTACGCCGTCTTGGGTATAAAACTCACCGCGCACaaa                                                                                 >  2:1181934/1‑150 (MQ=255)
                                                                       aTGCCTATAACGCGTAACAAGCACTGCAAAAAACAGCCGGACGGTTTTCACCTCCGGCTATTTTTTTAATTGTGATTACGCATCACCCCTTCCTGAACGGTCGAGGCAACCAGTACGCCGTCTTGGGTATAAAACTCACCGCGCACAAAg                                                                                <  1:2740144/150‑1 (MQ=255)
                                                                        tGCCTATAACGCGTAACAAGCACTGCAAAAAACAGCCGGACGGTTTTCACCTCCGGCTATTTTTTTAATTGTGATTACGCATCACCCCTTCCTGAACGGTCGAGGCAACCAGTACGCCGTCTTGGGTATAAAACTCACCGCGCACAAAGc                                                                               <  1:4610314/150‑1 (MQ=255)
                                                                            tataACGCGTAACAAGCACTGCAAAAAACAGCCGGACGGTTTTCACCTCCGGCTATTTTTTTAATTGTGATTACGCATCACCCCTTCCTGAACGGTCGAGGCAACCAGTACGCCGTCTTGGGTATAAAACTCACCGCGCACAAAGCCACg                                                                           <  2:4134640/150‑1 (MQ=255)
                                                                              taACGCGTAACAAGCACTGCAAAAAACAGCCGGACGGTTTTCACCTCCGGCTATTTTTTTAATTGTGATTACGCATCACCCCTTCCTGAACGGTCGAGGCAACCAGTACGCCGTCTTGGGTATAAAACTCACCGCGCACAAAGCCACGTg                                                                         <  2:3880107/150‑1 (MQ=255)
                                                                               aaCGCGTAACAAGCACTGCAAAAAACAGCCGGACGGTTTTCACCTCCGGCTATTTTTTTAATTGTGATTACGCATCACCCCTTCCTGAACGGTCGAGGCAACCAGTACGCCGTCTTGGGTATAAAACTCACCGCGCACAAAGCCACGTgc                                                                        >  1:3498362/1‑150 (MQ=255)
                                                                                   cgTAACAAGCACTGCAAAAAACAGCCGGACGGTTTTCACCTCCGGCTATTTTTTTAATTGTGATTACGCATCACCCCTTCCTGAACGGTCGAGGCAACCAGTACGCCGTCTTGGGTATAAAACTCACCGCGCACAAAGCCACGTGCGCTg                                                                    <  2:730594/150‑1 (MQ=255)
                                                                                    gTAACAAGCACTGCAAAAAACAGCCGGACGGTTTTCACCTCCGGCTATTTTTTTAATTGTGATTACGCATCACCCCTTCCTGAACGGTCGAGGCAACCAGTACGCCGTCTTGGGTATAAAACTCACCGCGCACAAAGCCACGTGCGCTgg                                                                   >  1:3687422/1‑150 (MQ=255)
                                                                                      aaCAAGCACTGCAAAAAACAGCCGGACGGTTTTCACCTCCGGCTATTTTTTTAATTGTGATTACGCATCACCCCTTCCTGAACGGTCGAGGCAACCAGTACGCCGTCTTGGGTATAAAACTCACCGCGCACAAAGCCACGTGCGCTGGAc                                                                 <  1:2359286/150‑1 (MQ=255)
                                                                                        cAAGCACTGCAAAAAACAGCCGGACGGTTTTCACCTCCGGCTATTTTTTTAATTGTGATTACGCATCACCCCTTCCTGAACGGTCGAGGCAACCAGTACGCCGTCTTGGGTATAAAACTCACCGCGCACAAAGCCACGTGCGCTGGACGc                                                               >  1:1896799/1‑150 (MQ=255)
                                                                                         aaGCACTGCAAAAAACAGCCGGACGGTTTTCACCTCCGGCTATTTTTTTAATTGTGATTACGCATCACCCCTTCCTGAACGGTCGAGGCAACCAGTACGCCGTCTTGGGTATAAAACTCACCGCGCACAAAGCCACGTGCGCTGGACGcc                                                              <  1:1983278/150‑1 (MQ=255)
                                                                                          aGCACTGCAAAAAACAGCCGGACGGTTTTCACCTCCGGCTATTTTTTTAATTGTGATTACGCATCACCCCTTCCTGAACGGTCGAGGCAACCAGTACGCCGTCTTGGGTATAAAACTCACCGCGCACAAAGCCACGTGCGCTGGACGCCg                                                             <  2:2841499/150‑1 (MQ=255)
                                                                                           gCACTGCAAAAAACAGCCGGACGGTTTTCACCTCCGGCTATTTTTTTAATTGTGATTACGCATCACCCCTTCCTGAACGGTCGAGGCAACCAGTACGCCGTCTTGGGTATAAAACTCACCGCGCACAAAGCCACGTGCGCTGGACGCCGa                                                            >  2:1376676/1‑150 (MQ=255)
                                                                                            cACTGCAAAAAACAGCCGGACGGTTTTCACCTCCGGCTATTTTTTTAATTGTGATTACGCATCACCCCTTCCTGAACGGTCGAGGCAACCAGTACGCCGTCTTGGGTATAAAACTCACCGCGCACAAAGCCACGTGCGCTGGACGCCGAg                                                           <  1:3898439/150‑1 (MQ=255)
                                                                                             aCTGCAAAAAACAGCCGGACGGTTTTCACCTCCGGCTATTTTTTTAATTGTGATTACGCATCACCCCTTCCTGAACGGTCGAGGCAACCAGTACGCCGTCTTGGGTATAAAACTCACCGCGCACAAAGCCACGTGCGCTGGACGCCGAgg                                                          <  1:2320405/150‑1 (MQ=255)
                                                                                              cTGCAAAAAACAGCCGGACGGTTTTCACCTCCGGCTATTTTTTTAATTGTGATTACGCATCACCCCTTCCTGAACGGTCGAGGCAACCAGTACGCCGTCTTGGGTATAAAACTCACCGCGCACAAAGCCACGTGCGCTGGACGCCGAGGt                                                         >  1:509231/1‑150 (MQ=255)
                                                                                                gccaacaaCAGCCGGACGGTGTTCACCTCCGGCTATTTTTTTAATTGTGCTTACGCATCACCCCGTCCTGAACGGTCGAGGCAACCAGGACGCCGTCTTGGGTATACAACTCACCGCGCACACAGCCACGTGCGCTGGACGCCGAGGTGc                                                       <  2:4310305/144‑1 (MQ=255)
                                                                                                gCAAAAAACAGCCGGACGGTTTTCACCTCCGGCTATTTTTTTAATTGTGATTACGCATCACCCCTTCCTGAACGGTCGAGGCAACCAGTACGCCGTCTTGGGTATAAAACTCACCGCGCACAAAGCCACGTGCGCTGGACGCCGAGGTGc                                                       <  1:4911921/150‑1 (MQ=255)
                                                                                                gCAAAAAACAGCCGGACGGTTTTCACCTCCGGCTATTTTTTTAATTGTGATTAAGCATCACCCCTTCCTGAACGGTCGAGGCAACCAGTACGCCGTCTTGGGTATAAAACTCACCGCGCACAAAGCCACGTGCGCTGGACGCCGAGGTGc                                                       >  2:4080449/1‑150 (MQ=255)
                                                                                                 cAAAAAACAGCCGGACGGTTTTCACCTCCGGCTATTTTTTTAATTGTGATTACGCATCACCCCTTCCTGAACGGTCGAGGCAACCAGTACGCCGTCTTGGGTATAAAACTCACCGCGCACAAAGCCACGTGCGCTGGACGCCGAGGTGct                                                      <  2:2248034/150‑1 (MQ=255)
                                                                                                  aaaaaaCAGCCGGACGGTTTTCACCTCCGGCTATTTTTTTAATTGTGATTACGCATCACCCCTTCCTGAACGGTCGAGGCAACCAGTACGCCGTCTTGGGTATAAAACTCACCGCGCACAAAGCCACGTGCGCTGGACGCCGAGGTGctc                                                     >  2:459152/1‑150 (MQ=255)
                                                                                                    aaaaCAGCCGGACGGTTTTCACCTCCGGCTATTTTTTTAATTGTGATTAAGCATCACCCCTTCCTGAACGGTCGAGGCAACCAGTACGCCGTCTTGGGTATAAAACTCACCGCGCACAAAGCCACGTGCGCTGGACGCCGAGGTGCtctc                                                   >  1:2902405/1‑150 (MQ=255)
                                                                                                      aaCAGCCGGACGGTTTTCACCTCCGGCTATTTTTTTAATTGTGATTACGCATCACCCCTTCCTGAACGGTCGAGGCAACCAGTACGCCGGCTTGGGTATAAAACTCACCGCGCACAAAGCCACGTGCGCTGGACGCCGAGGTGCTCTCCa                                                 >  1:3716233/1‑150 (MQ=255)
                                                                                                       aCAGCCGGACGGTTTTCACCTCCGGCTATTTTTTTAATTGTGATTACGCATCACCCCTTCCTGAACGGTCGAGGCAACCAGTACGCCGTCTTGGGTATAAAACTCACCGCGCACAAAGCCACGTGCGCTGGACGCCGAGGTGCTCTCCAc                                                >  1:4438830/1‑150 (MQ=255)
                                                                                                       aCAGCCGGACGGTTCTCACCTCCGGCTATTTTTTTAATTGTGATTACGCATCACCCCTTCCTGAACGGTCGAGGCAACCAGTACGCCGTCTTGGGTATAAAACTCACCGCGCACAAAGCCACGTGCGCTGGACGCCGAGGTGCTCTCCAc                                                <  2:1717379/150‑1 (MQ=255)
                                                                                                         aGCCGGACGGTTTTCACCTCCGGCTATTTTTTTAATTGTGATTACGCATCACCCCTTCCTGAACGGTCGAGGCAACCAGTACGCCGTCTTGGGTATAAAACTCACCGCGCACAAAGCCACGTGCGCTGGACGCCGAGGTGCTCTCCACGc                                              >  1:699120/1‑150 (MQ=255)
                                                                                                          gcgggACGGTTTTCACCTCCGGCTATTTTTTTAATTGTGATTACGCATCACCCCTTCCTGAACGGTAGAGGCAACCAGTACGCCGACTTGGGTATAAAACTCACCGCGCACAAAGCCACGTGCGCTGGACGCCGAGGTGCTCTCCACGCt                                             >  1:2088278/4‑150 (MQ=255)
                                                                                                          gCCGGACGGTTTTCACCTCCGGCTATTTTTTTAATTGTGATTACGCATCACCCCTTCCTGAACGGTCGAGGCAACCAGTACGCCGTCTTGGGTATAAAACTCACCGCGCACAAAGCCACGTGCGCTGGACGCCGAGGTGCTCTCCACGCt                                             >  1:4580893/1‑150 (MQ=255)
                                                                                                          gCCGGACGGTTTTCACCTCCGGCTATTTTTTTAATTGTGATTACGCATCACCCCTTCCTGAACGGTCGAGGCAACCAGTACGCCGTCTTGGGTATAAAACTCACCGCGCACAAAGCCACGTGCGCTGGACGCCGAGGTGCTCTCCACGCt                                             <  1:546604/150‑1 (MQ=255)
                                                                                                           ccGGACGGTTTTCACCTCCGGCTATTTTTTTAATTGTGATTACGCATCACCCCTTCCTGAACGGTCGAGGCAACCAGTACGCCGTCTTGGGTATAAAACTCACCGCGCACAAAGCCACGTGCGCTGGACGCCGAGGTGCTCTCCACGCta                                            <  2:1347385/150‑1 (MQ=255)
                                                                                                                 ggTTTTCACCTCCGGCTATTTTTTTAATTGTGATTACGCATCACCCCTTCCTGAACGGTCGAGGCAACCAGTACGCCGTCTTGGGTATAAAACTCACCGCGCACAAAGCCACGTGCGCTGGACGCCGAGGTGCTCTCCACGCTATAcagc                                      >  1:1522170/1‑150 (MQ=255)
                                                                                                                 ggTTTTCACCTCCGGCTATTTTTTTAATTGTGATTAAGCATCACCCCTTCCTGAACGGTCGAGGCAACCAGTACGCCGTCTTGGGTATAAAACTCACCGCGCACAAAGCCACGTGCGCTGGACGCCGAGGTGCTCTCCACGCTATAcagc                                      >  1:2491706/1‑150 (MQ=255)
                                                                                                                   ttttCACCTCCGGCTATTTTTTTAATTGTGATTACGCATCACCCCTTCCTGAACGGTCGAGGCAACCAGTACGCCGTCTTGGGTATAAAACTCACCGCGCACAAAGCCACGTGCGCTGGACGCCGAGGTGCTCTCCACGCTATAcagcag                                    <  2:2106632/150‑1 (MQ=255)
                                                                                                                    tttCACCTCCGGCTATTTTTTTAATTGTGATTACGCATCACCCCTTCCTGAACGGTCGAGGCAACCAGTACGCCGTCTTGGGTATAAAACTCACCGCGCACAAAGCCACGTGCGCTGGACGCCGAGGTGCTCTCCACGCTATACagcagc                                   >  2:3405038/1‑150 (MQ=255)
                                                                                                                    tttCACCTCCGGCTATTTTTTTAATTGTGATTAAGCATCACCCCTTCCTGAACGGTCGAGGCAACCAGTACGCCGTCTTGGGTATAAAACTCACCGCGCACAAAGCCACGTGCGCTGGACGCCGAGGTGCTCTCCACGCTATACagcagc                                   <  1:2708857/150‑1 (MQ=255)
                                                                                                                     ttCACCTCCGGCTATTTTTTTAATTGTGATTAAGCATCACCCCTTCCTGAACGGTCGAGGCAACCAGTACGCCGTCTTGGGTATAAAACTCACCGCGCACAAAGCCACGTGCGCTGGACGCCGAGGTGCTCTCCACGCTATACAGCAGcc                                  <  1:416609/150‑1 (MQ=255)
                                                                                                                      tCACCTCCGGCTATTTTTTTAATTGTGATTACGCATCACCCCTTCCTGAACGGTCGAGGCAACCAGTACGCCGTCTTGGGTATAAAACTCACCGCGCACAAAGCCACGTGCGCTGGACGCCGAGGTGCTCTCCACGCTATACAGCAGCca                                 >  1:1456975/1‑150 (MQ=255)
                                                                                                                        aCCTCCGGCTATTTTTTTAATTGTGATTACGCATCACCCCTTCCTGAACGGTCGAGGCAACCAGTACGCCGTCTTGGGTATAAAACTCACCGCGCACAAAGCCACGTGCGCTGGACGCCGAGGTGCTCTCCACGCTATACAGCAGCcatt                               >  2:2988259/1‑150 (MQ=255)
                                                                                                                        aCCTCCGGCTATTTTTTTAATTGTGATTACGCATCACCCCTTCCTGAACGGTCGAGGCAACCAGTACGCCGTCTTGGGTATAAAACTCACCGCGCACAAAGCCACGTGCGCTGGACGCCGAGGTGCTCTCCACGCTATACAGCAGCcatt                               <  1:3374756/150‑1 (MQ=255)
                                                                                                                          cTCCGGCTATTTTTTTAATTGTGATTACGCATCACCCCTTCCTGAACGGTCGAGGCAACCAGTACGCCGTCTTGGGTATAAAACTCACCGCGCACAAAGCCACGTGCGCTGGACGCCGAGGTGCACTCCACGCTATACAGCAGCcattca                             <  2:2602753/150‑1 (MQ=255)
                                                                                                                           tCCGGCTATTTTTTTAATTGTGATTACGCATCACCCCTTCCTGAACGGTCGAGGCAACCAGTACGCCGTCTTGGGTATAAAACTCACCGCGCACAAAGCCACGTGCGCTGGACGCCGAGGTGCTCTCCACGCTATACAGCAGCcattcat                            >  2:3239160/1‑150 (MQ=255)
                                                                                                                            ccGGCTATTTTTTTAATTGTGATTACGCATCACCCCTTCCTGAACGGTCGAGGCAACCAGTACGCCGTCTTGGGTATAAAACTCACCGCGCACAAAGCCACGTGCGCTGGACGCCGAGGTGCTCTCCACGCTATACAGCAGCcattcatt                           >  2:1709274/1‑150 (MQ=255)
                                                                                                                             cGGCTATTTTTTTAATTGTGATTACGCATCACCCCTTCCTGAACGGTCGAGGCAACCAGTACGCCGTCTTGGGTATAAAACTCACCGCGCACAAAGCCACGTGCGCTGGACGCCGAGGTGCTCTCCACGCTATACAGCAGCCattcattc                          <  2:3606142/150‑1 (MQ=255)
                                                                                                                             cGGCTATTTTTTTAATTGTGATTAAGCATCACCCCTTCCTGAACGGTCGAGGCAACCAGTACGCCGTCTTGGGTATAAAACTCACCGCGCACAAAGCCACGTGCGCTGGACGCCGAGGTGCTCTCCACGCTATACAGCAGCCattcattc                          <  1:3593820/150‑1 (MQ=255)
                                                                                                                              ggCTATTTTTTTAATTGTGATTACGCATCACCCCTTCCTGAACGGTCGAGGCAACCAGTACGCCGTCTTGGGTATAAAACTCACCGCGCACAAAGCCACGTGCGCTGGACGCCGAGGTGCTCTCCACGCTATACAGCAGCCAttcattca                         >  2:2613140/1‑150 (MQ=255)
                                                                                                                              ggCTATTTTTTTAATTGTGATTACGCATCACCCCTTCCTGAACGGTCGAGGCAACCAGTACGCCGTCTTGGGTATAAAACTCACCGCGCACAAAGCCACGTGCGCTGGACGCCGAGGTGCTCTCCACGCTATACAGCAGCCAttcattca                         >  1:3141371/1‑150 (MQ=255)
                                                                                                                                 tATTTTTTTAATTGTGATTACGCATCACCCCTTCCTGAACGGTCGAGGCAACCAGTACGCCGTCTTGGGTATAAAACTCACCGCGCACAAAGCCACGTGCGCTGGACGCCGAGGTGCTCTCCACGCTATACAGCAGCCATTCATTCAAAt                      <  2:131923/150‑1 (MQ=255)
                                                                                                                                  aTTTTTTTAATTGTGATTACGCATCACCCCTTCCTGAACGGTCGAGGCAACCAGTACGCCGTCTTGGGTATAAAACTCACCGCGCACAAAGCCACGTGCGCTGGACGCCGAGGTGCTCTCCACGCTATACAGCAGCCATTCATTCAAAtt                     <  2:2777514/150‑1 (MQ=255)
                                                                                                                                   tttttttAATTGTGATTACGCATCACCCCTTCCTGAACGGTCGAGGCAACCAGTACGCCGTCTTGGGTATAAAACTCACCGCGCACAAAGCCACGTGCGCTGGACGCCGAGGTGCTCTCCACGCTATACAGCAGCCATTCATTCAAATTa                    <  2:428318/150‑1 (MQ=255)
                                                                                                                                   tttttttAATTGTGATTACGCATCACCCCTTCCTGAACGGTCGAGGCAACCAGTACGCCGTCTTGGGTATAAAACTCACCGCGCACAAAGCCACGTGCGCTGGACGCCGAGGTGCTCTCCACGCTATACAGCAGCCATTCATTCAAATTa                    <  1:1268841/150‑1 (MQ=255)
                                                                                                                                   attttttAATTGTGATTACTCATAACCCCTTCCTGAACGGTAGAGGCAACCAGTACGACTTCTATGGTATAAAACTCACCGCTCACAAAGCCACGTGCGCTGGACGCCGAGGTGCTCTACACGCTATACAGAAGCCATTCATTCAAATTa                    >  2:34833/2‑150 (MQ=255)
                                                                                                                                      ttttAATTGTGATTACGCATCACCCCTTCCTGAACGGTCGAGGCAACCAGTACGCCGTCTTGGGTATAAAACTCACCGCGCACAAAGCCACGTGCGCTGGACGCCGAGGTGCTCTCCACGCTATACAGCAGCCATTCATTCAAATTAAAc                 <  1:2204741/150‑1 (MQ=255)
                                                                                                                                        ttAATTGTGATTACGCATCACCCCTTCCTGAACGGTCGAGGCAACCAGTACGCCGTCTTGGGTATAAAACTCACCGCGCACAAAGCCACGTGCGCTGGACGCCGAGGTGCTCTCCACGCTATACAGCAGCCATTCATTCAAATTAAACgg               >  1:4375742/1‑150 (MQ=255)
                                                                                                                                          aaTTGTGATTACGCATCACCCCTTCCTGAACGGTCGAGGCAACCAGTACGCCGTCTTGGGTATAAAACTCACCGCGCACAAAGCCACGTGCGCTGGACGCCGAGGTGCTCTCCACGCTATACAGCAGCCATTCATTCAAATTAAACGGGc             <  2:4374514/150‑1 (MQ=255)
                                                                                                                                          aaTTGTGATTACGCATCACCCCTTCCTGAACGGTCGAGGCAACCAGTACGCCGTCTTGGGTATAAAACTCACCGCGCACAAAGCCACGTGCGCTGGACGCCGAGGTGCTCTCCACGCTATACAGCAGCCATTCATTCAAATTAAACGGGc             >  2:1194555/1‑150 (MQ=255)
                                                                                                                                           aTTGTGATTACGCATCACCCCTTCCTGAACGGTCGAGGCAACCAGTACGCCGTCTTGGGTATAAAACTCACCGCGCACAAAGCCACGTGCGCTGGACGCCGAGGTGCTCTCCACGCTATACAGCAGCCATTCATTCAAATTAAACGGGCg            >  2:2552453/1‑150 (MQ=255)
                                                                                                                                            atgtgATTACGAATCACCCCATCCTGAACTGTCTAGGCAACAAGAACGCCTTCTTGGGTAAAAAACTCACCGCGCACAAAGCCACGTGCGCTGGAAGCCGAGGTGCTCTCAACGCTATACAGCAGCCATACATTCAAATAAAAAggccta           >  2:462533/2‑146 (MQ=255)
                                                                                                                                              gtgATTACGCATCACCCCTTCCTGAACGGTCGAGGCAACCAGTACGCCGTCTTGGGTATAAAACTCACCGCGCACAAAGCCACGTGCGCTGGACGCCGAGGTGCTCTCCACGCTATACAGCAGCCATTCATTCAAATTAAACGGGCGATg         >  2:273131/1‑150 (MQ=255)
                                                                                                                                              gtgATTACGCATCACCCCTTCCTGAACGGTCGAGGCAACCAGTACGCCGTCTTGGGTATAAAACTCACCGCGCACAAAGCCACGTGCGCTGGACGCCGAGGTGCTCTCCACGCTATACAGCAGCCATTCATTCAAATTAAACGGGCGATg         <  1:5015110/150‑1 (MQ=255)
                                                                                                                                                 aTTACGCATCACCCCTTCCTGAACGGTCGAGGCAACCAGTACGCCGTCTTGGGTATAAAACTCACCGCGCACAAAGCCACGTGCGCTGGACGCCGAGGTGCTCTCCACGCTATACAGCAGCCATTCATTCAAATTAAACGGGCGATGGaa      >  2:3964570/1‑150 (MQ=255)
                                                                                                                                                 aTTACGCATCACCCCTTCCTGAACGGTCGAGGCAACCAGTACGCCGTCTTGGGTATAAAACTCACCGCGCACAAAGCCACGTGCGCTGGACGCCGAGGTGCTCTCCACGCTATACAGCAGCCATTCATTCAAATTAAACGGGCGATGGaa      >  2:1071680/1‑150 (MQ=255)
                                                                                                                                                    aCGCATCACCCCTTCCTGAACGGTCGAGGCAACCAGTACGCCGTCTTGGGTATAAAACTCACCGCGCACAAAGCCACGTGCGCTGGACGCCGAGGTGCTCTCCACGCTATACAGCAGCCATTCATTCAAATTAAACGGGCGCTGGAACca   >  2:76837/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
TCTGACGGTTGGTCTGCGTGTACCGGAAGAGCAGGAGCGAGAAGGGCTGGATGTCAACAGCCACGGCGAGAATGCCTATAACGCGTAACAAGCACTGCAAAAAACAGCCGGACGGTTTTCACCTCCGGCTATTTTTTTAATTGTGATTACGCATCACCCCTTCCTGAACGGTCGAGGCAACCAGTACGCCGTCTTGGGTATAAAACTCACCGCGCACAAAGCCACGTGCGCTGGACGCCGAGGTGCTCTCCACGCTATACAGCAGCCATTCATTCAAATTAAACGGGCGATGGAACCAC  >  CP009273/469621‑469919

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑