Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 55,590 G→T 90.4% T507K (ACG→AAG)  lptD ← LPS assembly OM complex LptDE, beta‑barrel component

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP00927355,5900GT90.4% 876.5 / 91.2 323T507K (ACG→AAG) lptDLPS assembly OM complex LptDE, beta‑barrel component
Reads supporting (aligned to +/- strand):  ref base G (12/19);  new base T (123/169);  total (135/188)
Fisher's exact test for biased strand distribution p-value = 8.49e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.26e-01

Only 100 of 327 total aligned reads displayed.

TTAGCGGAGGCAATACGGTCAAGACCGCCGTAAGTCCGGTCCCGGAACAGGCCAGAGTAGTCAGATTGCAGCAGAGACGAGTCGTAGTTATAGATGTCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCGTTTGGGTATAACCCGGAGCCAGCATTTCCATATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCGGCATTACGCGGTTAACGGATTCGTCCAGCTTGGTCGTGTTTCTGGAGTTATACCAGTCAAGATTGGTTTGCTGA  >  CP009273/55443‑55736
                                                                                                                                                   |                                                                                                                                                  
ttAGCGGAGGCAATACGGTCAAGACCGCCGTAAGTCCGGTCCCGGAACAGGCCAGAGTAGTCAGATTGCAGCAGAGACGAGTCGTAGTTATAGATGTCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCttt                                                                                                                                                  <  2:4033814/150‑1 (MQ=255)
 tAGCGGAGGCAATACGGTCAAGACCGCCGTAAGTCCGGTCCCGGAACAGGCCAGAGTAGTCAGATTGCAGCAGAGACGAGTCGTAGTTATAGATGTCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCtttt                                                                                                                                                 <  2:4355645/150‑1 (MQ=255)
    cGGAGGCAATACGGTCAAGACCGCCGTAAGTCCGGTCCCGGAACAGGCCAGAGTAGTCAGATTGCAGCAGAGACGAGTCGTAGTTATAGATGTCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCTTTTggg                                                                                                                                              >  1:79427/1‑150 (MQ=255)
       aGGCAATACGGTCAAGACCGCCGTAAGTCCGGTCCCGGAACAGGCCAGAGTAGTCAGATTGCAGCAGAGACGAGTCGTAGTTATAGATGTCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCTTTTGGGtat                                                                                                                                           >  2:4978490/1‑150 (MQ=255)
        ggCAATACGGTCAAGACCGCCGTAAGTCCGGTCCCGGAACAGGCCAGAGTAGTCAGATTGCAGCAGAGACGAGTCGTAGTTATAGATGTCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCTTTTGGGtata                                                                                                                                          <  2:2449055/150‑1 (MQ=255)
         gCAATACGGTCAAGACCGCCGTAAGTCCGGTCCCGGAACAGGCCAGAGTAGTCAGATTGCAGCAGAGACGAGTCGTAGTTATAGATGTCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCTTTTGGGTATaa                                                                                                                                         >  1:2348642/1‑150 (MQ=255)
            aTACGGTCAAGACCGCCGTAAGTCCGGTCCCGGAACAGGCCAGAGTAGTCAGATTGCAGCAGAGACGAGTCGTAGTTATAGATGTCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCTTTTGGGTATAAccc                                                                                                                                      <  2:1004915/150‑1 (MQ=255)
            aTACGGTCAAGACCGCCGTAAGTCCGGTCCCGGAACAGGCCAGAGTAGTCAGATTGCAGCAGAGACGAGTCGTAGTTATAGATGTCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCTTTTGGGTATAAccc                                                                                                                                      <  2:104669/150‑1 (MQ=255)
            aTACGGTCAAGACCGCCGTAAGTCCGGTCCCGGAACAGGCCAGAGTAGTCAGATTGCAGCAGAGACGAGTCGTAGTTATAGATGTCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCGTTTGGGTATAAccc                                                                                                                                      <  2:1561076/150‑1 (MQ=255)
              aCGGTCAAGACCGCCGTAAGTCCGGTCCCGGAACAGGCCAGAGTAGTCAGATTGCAGCAGAGACGAGTCGTAGTTATAGATGTCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCTTTTGGGTATAACCCgg                                                                                                                                    >  1:1478282/1‑150 (MQ=255)
                ggTCAAGACCGCCGTAAGTCCGGTCCCGGAACAGGCCAGAGTAGTCAGATTGCAGCAGAGACGAGTCGTAGTTATAGATGTCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCTTTTGGGTATAACCCGGAg                                                                                                                                  >  1:4681694/1‑150 (MQ=255)
                   cAAGACCGCCGTAAGTCCGGTCCCGGAACAGGCCAGAGTAGTCAGATTGCAGCAGAGACGAGTCGTAGTTATAGATGTCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCTTTTGGGTATAACCCGGAGCCa                                                                                                                               <  2:4496861/150‑1 (MQ=255)
                   cAAGACCGCCGTAAGTCCGGTCCCGGAACAGGCCAGAGTAGTCAGATTGCAGCAGAGACGAGTCGTAGTTATAGATGTCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCTTTTGGGTATAACCCGGAGCCa                                                                                                                               <  2:4485872/150‑1 (MQ=255)
                   cAAGACCGCCGTAAGTCCGGTCCCGGAACAGGCCAGAGTAGTCAGATTGCAGCAGAGACGAGTCGTAGTTATAGATGTCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCTTTTGGGTATAACCCGGAGCCa                                                                                                                               <  1:1242989/150‑1 (MQ=255)
                   cAAGACCGCCGTAAGTCCGGTCCCGGAACAGGCCAGAGTAGTCAGATTGCAGCAGAGACGAGTCGTAGTTATAGATGTCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCTTTTGGGTATAACCCGGAGCCa                                                                                                                               >  2:190265/1‑150 (MQ=255)
                   cAAGACCGCCGTAAGTCCGGTCCCGGAACAGGCCAGAGTAGTCAGATTGCAGCAGAGACGAGTCGTAGTTATAGATGTCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCGTTTGGGTATAACCCGGAGCCa                                                                                                                               <  1:814280/150‑1 (MQ=255)
                     aGACCGCCGTAAGTCCGGTCCCGGAACAGGCCAGAGTAGTCAGATTGCAGCAGAGACGAGTCGTAGTTATAGATGTCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCGTTTGGGTATAACCCGGAGCCAGc                                                                                                                             >  1:581033/1‑150 (MQ=255)
                     aGACCGCCGTAAGTCCGGTCCCGGAACAGGCCAGAGTAGTCAGATTGCAGCAGAGACGAGTCGTAGTTATAGATGTCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCGTTTGGGTATAACCCGGAGCCAGc                                                                                                                             >  2:2276630/1‑150 (MQ=255)
                        ccgccgTAAGTCCGGTCCCGGAACAGGCCAGAGTAGTCAGATTGCAGCAGAGACGAGTCGTAGTTATAGATGTCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCTTTTGGGTATAACCCGGAGCCAGCAtt                                                                                                                          <  2:1341673/150‑1 (MQ=255)
                         cgccgTAAGTCCGGTCCCGGAACAGGCCAGAGTAGTCAGATTGCAGCAGAGACGAGTCGTAGTTATAGATGTCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCTTTTGGGTATAACCCGGAGCCAGCAttt                                                                                                                         <  2:3008663/150‑1 (MQ=255)
                           ccgTAAGTCCGGTCCCGGAACAGGCCAGAGTAGTCAGATTGCAGCAGAGACGAGTCGTAGTTATAGATGTCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCTTTTGGGTATAACCCGGAGCCAGCATTTcc                                                                                                                       <  2:3153648/150‑1 (MQ=255)
                             gTAAGTCCGGTCCCGGAACAGGACAGAGTAGTCAGATTGCAGCAGAGACGAGTCGTAGTTATAGATGTCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCTTTTGGGTATAACCCGGAGCCAGCATTTCCat                                                                                                                     <  1:3918259/150‑1 (MQ=255)
                                aGTCCGGTCCCGGAACAGGCCAGAGTAGTCAGATTGCAGCAGAGACGAGTCGTAGTTATAGATGTCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCTTTTGGGTATAACCCGGAGCCAGCATTTCCATATc                                                                                                                  <  1:82900/150‑1 (MQ=255)
                                aGTCCGGTCCCGGAACAGGCCAGAGTAGTCAGATAGCAGCAGAGACGAGACGTAGTAAAAGATGTCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCTTTTGGGTATAACACGGAGCCAGCATTTCCATATc                                                                                                                  >  1:4935213/1‑150 (MQ=255)
                                     ggTCCCGGAACAGGCCAGAGTAGTCAGATTGCAGCAGAGACGAGTCGTAGTTATAGATGTCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCTTTTGGGTATAACCCGGAGCCAGCATTTCCATATCGCGtt                                                                                                             <  1:5124036/150‑1 (MQ=255)
                                      gTCCCGGAACAGGCCAGAGTAGTCAGATTGCAGCAGAGACGAGTCGTAGTTATAGATGTCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCTTTTGGGTATAACCCGGAGCCAGCATTTCCATATCGCGTTc                                                                                                            <  1:5079569/150‑1 (MQ=255)
                                       tCCCGGAACAGGCCAGAGTAGTCAGATTGCAGCAGAGACGAGTCGTAGTTATAGATGTCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCTTTTGGGTATAACCCGGAGCCAGCATTTCCATATCGCGTTCa                                                                                                           <  1:4160968/150‑1 (MQ=255)
                                         ccGGAACAGGCCAGAGTAGTCAGATTGCAGCAGAGACGAGTCGTAGTTATAGATGTCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCTTTTGGGTATAACCCGGAGCCAGCATTTCCATATCGCGTTCaaa                                                                                                         <  1:3991437/150‑1 (MQ=255)
                                           ggAACAGGCCAGAGTAGTCAGATTGCAGCAGAGACGAGTCGTAGTTATAGATGTCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCGTTTGGGTATAACCCGGAGCCAGCATTTCCATATCGCGTTCAAAGa                                                                                                       <  2:3211037/150‑1 (MQ=255)
                                             aaCAGGCCAGAGTAGTCAGATTGCAGCAGAGACGAGTCGTAGTTATAGATGTCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCTTTTGGGTATAACCCGGAGCCAGCATTTCCATATCGCGTTCAAAGAcc                                                                                                     >  1:2937580/1‑150 (MQ=255)
                                                aGGCCAGAGTAGTCAGATTGCAGCAGAGACGAGTCGTAGTTATAGATGTCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCTTTTGGGTATAACCCGGAGCCAGCATTTCCATATCGCGTTCAAAGACCAtt                                                                                                  <  2:2009871/150‑1 (MQ=255)
                                                   ccAGAGTAGTCAGATTGCAGCAGAGACGAGTCGTAGTTATAGATGTCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCTTTTGGGTATAACCCGGAGCCAGCATTTCCATATCGCGTTCAAAGACCATTTTg                                                                                               >  1:221352/1‑150 (MQ=255)
                                                     agagTAGTCAGATTGCAGCAGAGACGAGTCGTAGTTATAGATGTCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCTTTTGGGTATAACCCGGAGCCAGCATTTCCATATCGCGTTCAAAGACCATTTTGcc                                                                                             >  1:317056/1‑150 (MQ=255)
                                                       agtagtCAGATTGCAGCAGAGACGAGTCGTAGTTATAGATGTCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCTTTTGGGTATAACCCGGAGCCAGCATTTCCATATCGCGTTCAAAGACCATTTTGCCGt                                                                                           <  1:3342958/150‑1 (MQ=255)
                                                       agtagtCAGATTGCAGCAGAGACGAGTCGTAGTTATAGATGTCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCTTTTGGGTATAACCCGGAGCCAGCATTTCCATATCGCGTTCAAAGACCATTTTGCCGt                                                                                           >  2:3987607/1‑150 (MQ=255)
                                                       agtagtCAGATTGCAGCAGAGACGAGTCGTAGTTATAGATGTCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCTTTTGGGTATAACCCGGAGCCAGCATTTCCATATCGCGTTCAAAGACCATTTTGCCGt                                                                                           >  2:4813646/1‑150 (MQ=255)
                                                          agtCAGATTGCAGCAGAGACGAGTCGTAGTTATAGATGTCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCTTTTGGGTATAACCCGGAGCCAGCATTTCCATATCGCGTTCAAAGACCATTTTGCCGTCaa                                                                                        >  1:1404361/1‑150 (MQ=255)
                                                           gtCAGATTGCAGCAGAGACGAGTCGTAGTTATAGATGTCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCTTTTGGGTATAACCCGGAGCCAGCATTTCCATATCGCGTTCAAAGACCATTTTGCCGTCAAc                                                                                       <  2:1822340/150‑1 (MQ=255)
                                                           gtCAGATTGCAGCAGAGACGAGTCGTAGTTATAGATGTCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCTTTTGGGTATAACCCGGAGCCAGCATTTCCATATCGCGTTCAAAGACCATTTTGCCGTCAAc                                                                                       >  1:3665106/1‑150 (MQ=255)
                                                             cAGATTGCAGCAGAGACGAGTCGTAGTTATAGATGTCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCTTTTGGGTATAACCCGGAGCCAGCATTTCCATATCGCGTTCAAAGACCATTTTGCCGTCAACtt                                                                                     <  1:1976715/150‑1 (MQ=255)
                                                              aGATTGCAGCAGAGACGAGTCGTAGTTATAGATGTCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCTTTTGGGTATAACCCGGAGCCAGCATTTCCATATCGCGTTCAAAGACCATTTTGCCGTCAACttt                                                                                    <  2:2831420/150‑1 (MQ=255)
                                                              aGATTGCAGCAGAGACGAGTCGTAGTTATAGATGTCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCTTTTGGGTATAACCCGGAGCCAGCATTTCCATATCGCGTTCAAAGACCATTTTGCCGTCAACttt                                                                                    >  2:628552/1‑150 (MQ=255)
                                                              aGATTGCAGCAGAGACGAGTCGTAGTTATAGATGTCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCTTTTGGGTATAACCCGGAGCCAGCATTTCCATATCGCGTTCAAAGACCATTTTGCCGTCAACttt                                                                                    <  1:4781063/150‑1 (MQ=255)
                                                                   gcagcaGAGACGAGTCGTAGTTATAGATGTCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCTTTTGGGTATAACCCGGAGCCAGCATTTCCATATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAAtt                                                                               <  2:2802083/150‑1 (MQ=255)
                                                                    cagcagAGACGAGTCGTAGTTATAGATGTCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCTTTTGGGTATAACCCGGAGCCAGCATTTCCATATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTg                                                                              >  1:526378/1‑150 (MQ=255)
                                                                     agcagAGACGAGTCGTAGTTATAGATGTCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCTTTTGGGTATAACCCGGAGCCAGCATTTCCATATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGc                                                                             >  1:3379227/1‑150 (MQ=255)
                                                                          agaCGAGTCGTAGTTATAGATGTCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCTTTTGGGTATAACCCGGAGCCAGCATTTCCATATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCGGCAt                                                                        <  2:4845670/150‑1 (MQ=255)
                                                                          agaCGAGTCGTAGTTATAGATGTCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCTTTTGGGTATAACCCGGAGCCAGCATTTCCATATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCGGCAt                                                                        <  2:79427/150‑1 (MQ=255)
                                                                           gaCGAGTCGTAGTTATAGATGTCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCTTTTGGGTATAACCCGGAGCCAGCATTTCCATATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCGGCAtt                                                                       >  2:4684828/1‑150 (MQ=255)
                                                                             cGAGTCGTAGTTATAGATGTCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCTTTTGGGTATAACCCGGAGCCAGCATTTCCATATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCGGCATTAc                                                                     <  1:1613205/150‑1 (MQ=255)
                                                                             cGAGTCGTAGTTATAGATGTCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCTTTTGGGTATAACCCGGAGCCAGCATTTCCATATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCGGCATTAc                                                                     <  1:1747052/150‑1 (MQ=255)
                                                                             cGAGTCGGAGTTATAGATGTCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCTTTTGGGTATAACCCGGAGCCAGCATTTCCATATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCGGCATTAc                                                                     <  1:3732947/150‑1 (MQ=255)
                                                                               aGTCGTAGTTATAGATGTCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCTTTTGGGTATAACCCGGAGCCAGCATTTCCATATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCGGCATTAcgc                                                                   >  2:2750400/1‑150 (MQ=255)
                                                                                gTCGTAGTTATAGATGTCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCTTTTGGGTATAACCCGGAGCCAGCATTTCCATATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCGGCATTAcgcg                                                                  >  2:366132/1‑150 (MQ=255)
                                                                                gTCGTAGTTAGAGATGTCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCTTTTGGGTATAACCCGGAGCCAGCATTTCCATATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCGGCATTAcgcg                                                                  <  2:3521271/150‑1 (MQ=255)
                                                                                 tCGTAGTTATAGATGTCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCTTTTGGGTATAACCCGGAGCCAGCATTTCCATATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCGGCATTACGCgg                                                                 >  2:4074932/1‑150 (MQ=255)
                                                                                 tCGTAGTTATAGATGTCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCTTTTGGGTATAACCCGGAGCCAGCATTTCCATATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCGGCATTACGCgg                                                                 <  2:4344990/150‑1 (MQ=255)
                                                                                  cGTAGTTATAGATGTCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCTTTTGGGTATAACCCGGAGCCAGCATTTCCATATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCGGCATTACGCGGt                                                                <  1:2708200/150‑1 (MQ=255)
                                                                                    tAGTTATAGATGTCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCTTTTGGGTATAACCCGGAGCCAGCATTTCCATATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCGGCATTACGCGGTTa                                                              >  2:159802/1‑150 (MQ=255)
                                                                                    tAGTTATAGATGTCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCGTTTGGGTATAACCCGGAGCCAGCATTTCCATATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCGGCATTACGCGGTTa                                                              >  1:4957494/1‑150 (MQ=255)
                                                                                         ataGATGTCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCTTTTGGGTATAACCCGGAGCCAGCATTTCCATATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCGGCATTACGCGGTTAACGGa                                                         >  1:4897879/1‑150 (MQ=255)
                                                                                         ataGATGTCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCTTTTGGGTATAACCCGGAGCCAGCATTTCCATATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCGGCATTACGCGGTTAACGGa                                                         <  2:3699608/150‑1 (MQ=255)
                                                                                          taGATGTCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCGTTTGGGTATAACCCGGAGCCAGCATTTCCATATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCGGCATTACGCGGTTAACGGAt                                                        <  1:3835522/150‑1 (MQ=255)
                                                                                             aTGTCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCTTTTGGGTATAACCCGGAGCCAGCATTTCCATATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCGGCATTACGCGGTTAACGGATTCg                                                     <  2:1750953/150‑1 (MQ=255)
                                                                                              tGTCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCTTTTGGGTATAACCCGGAGCCAGCATTTCCATATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCGGCATTACGCGGTTAACGGATTCGt                                                    <  2:5005893/150‑1 (MQ=255)
                                                                                               gTCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCTTTTGGGTATAACCCGGAGCCAGCATTTCCATATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCGGCATTACGCGGTTAACGGATTCGTc                                                   >  2:4455562/1‑150 (MQ=255)
                                                                                                tCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCTTTTGGGTATAACCCGGAGCCAGCATTTCCATATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCGGCATTACGCGGTTAACGGATTCGTcc                                                  <  2:2197553/150‑1 (MQ=255)
                                                                                                tCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCTTTTGGGTATAACCCGGAGCCAGCATTTCCATATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCGGCATTACGCGGTTAACGGATTCGTcc                                                  <  2:536984/150‑1 (MQ=255)
                                                                                                 cGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCTTTTGGGTATAACCCGGAGCCAGCATTTCCATATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCGGCATTACGCGGTTAACGGATTCGTCCa                                                 >  2:2133962/1‑150 (MQ=255)
                                                                                                   ctctGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCTTTTGGGTATAACCCGGAGCCAGCATTTCCATATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCGGCATTACGCGGTTAACGGATTCGTCCAGc                                               <  1:2579712/150‑1 (MQ=255)
                                                                                                    tctGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCTTTTGGGTATAACCCGGAGCCAGCATTTCCATATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCGGCATTACGCGGTTAACGGATTCGTCCAGCt                                              <  1:1814494/150‑1 (MQ=255)
                                                                                                    tctGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGGTCCAGCTTGTGGGTATAACCCGGAGCCAGCATTTCCATATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCGGCATTACGCGGTTAACGGATTCGTCCAGCt                                              <  1:4495845/150‑1 (MQ=255)
                                                                                                     ctGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCTTTTGGGTATAACCCGGAGCCAGCATTTCCATATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCGGCATTACGCGGTTAACGGATTCGTCCAGCtt                                             <  1:2805712/150‑1 (MQ=255)
                                                                                                     ctGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCTTGTGGGTATAACCCGGAGCCAGCATTTCCATATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCGGCATTACGCGGTTAACGGATTCGTCCAGCtt                                             <  2:3267257/150‑1 (MQ=255)
                                                                                                        aTCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCTTTTGGGTATAACCCGGAGCCAGCATTTCCATATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCGGCATTACGCGGTTAACGGATTCGTCCAGCTTGGt                                          <  1:3862786/150‑1 (MQ=255)
                                                                                                          cgcgATACGGCACGTACAACGACTGCGCGCGCGGTTCCAGCTTTTGGGTATAACCCGGAGCCAGCATTTCCATATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCGGCATTACGCGGTTAACGGATTCGTCCAGCTTGGTCg                                        <  2:4000894/150‑1 (MQ=255)
                                                                                                            cgATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCTTTTGGGTATAACCCGGAGCCAGCATTTCCATATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCGGCATTACGCGGTTAACGGATTCGTCCAGCTTGGTCgtg                                      <  1:4818553/150‑1 (MQ=255)
                                                                                                             gATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCTTTTGGGTATAACCCGGAGCCAGCATTTCCATATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCGGCATTACGCGGTTAACGGATTCGTCCAGCTTGGTCgtgt                                     >  2:5053368/1‑150 (MQ=255)
                                                                                                               tACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCTTTTGGGTATAACCCGGAGCCAGCATTTCCATATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCGGCATTACGCGGTTAACGGATTCGTCCAGCTTGGTCGTGttt                                   <  2:4755055/150‑1 (MQ=255)
                                                                                                                aCGGCACGTACAAATACTGCGCGCGCGGTTCCAGCTTTTGGGTATAACCCGGAGCCAGCATTTCCATATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCGGCATTACGCGGTTAACGGATTCGTCCAGCTTGGTCGTGTTTc                                  <  1:4374441/150‑1 (MQ=255)
                                                                                                                aCGGCACGTACAAATACTGCGCGCGCGGTTCCAGCTTTTGGGTATAACCCGGAGCCAGCATTTCCATATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCGGCATTACGCGGTTAACGGATTCGTCCAGCTTGGTCGTGTTTc                                  >  1:1989911/1‑150 (MQ=255)
                                                                                                                aCGGCACGTACAAATACTGCGCGCGCGGTTCCAGCGTTTGGGTATAACCCGGAGCCAGCATTTCCATATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCGGCATTACGCGGTTAACGGATTCGTCCAGCTTGGTCGTGTTTc                                  <  2:2702481/150‑1 (MQ=255)
                                                                                                                 cGGCACGTACAAATACTGCGCGCGCGGTTCCAGCTTTTGGGTATAACCCGGAGCCAGCATTTCCATATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCGGCATTACGCGGTTAACGGATTCGTCCAGCTTGGTCGTGTTTCt                                 <  1:2060044/150‑1 (MQ=255)
                                                                                                                   gCACGTACAAATACTGCGCGCGCGGTTCCAGCTTTTGGGTATAACCCGGAGCCAGCATTTCCATATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCGGCATTACGCGGTTAACGGATTCGTCCAGCTTGGTCGTGTTTCTgg                               <  2:117953/150‑1 (MQ=255)
                                                                                                                     aCGTACAAATACTGCGCGCGCGGTTCCAGCTTTTGGGTATAACCCGGAGCCAGCATTTCCATATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCGGCATTACGCGGTTAACGGATTCGTCCAGCTTGGTCGTGTTTCTGGAg                             >  1:3141934/1‑150 (MQ=255)
                                                                                                                      cGTACAAATACTGCGCGCGCGGTTCCAGCTTTTGGGTATAACCCGGAGCCAGCATTTCCATATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCGGCATTACGCGGTTAACGGATTCGTCCAGCTTGGTCGTGTTTCTGGAGt                            <  2:720290/150‑1 (MQ=255)
                                                                                                                        tACAAATACTGCGCGCGCGGTTCCAGCTTTTGGGTATAACCCGGAGCCAGCATTTCCATATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCGGCATTACGCGGTTAACGGATTCGTCCAGCTTGGTCGTGTTTCTGGAGTta                          <  1:1036327/150‑1 (MQ=255)
                                                                                                                        tACAAATACTGCGCGCGCGGTTCCAGCTTTTGGGTATAACCCGGAGCCAGCATTTCCATATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCGGCATTACGCGGTTAACGGATTCGTCCAGCTTGGTCGTGTTTCTGGAGTta                          >  2:2862794/1‑150 (MQ=255)
                                                                                                                        tACAAATACTGCGCGCGCGGTTCCAGCTTTTGGGTATAACCCGGAGCCAGCATTTCCATATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCGGCATTACGCGGTTAACGGATTCGTCCAGCTTGGTCGTGTTTCTGGAGTta                          >  2:1963397/1‑150 (MQ=255)
                                                                                                                          cAAATACTGCGCGCGCGGTTCCAGCTTTTGGGTATAACCCGGAGCCAGCATTTCCATATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCGGCATTACGCGGTTAACGGATTCGTCCAGCTTGGTCGTGTTTCTGGAGTtata                        <  2:4571005/150‑1 (MQ=255)
                                                                                                                           aaaTACTGCGCGCGCGGTTCCAGCTTTTGGGTATAACCCGGAGCCAGCATTTCCATATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCGGCATTACGCGGTTAACGGATTCGTCCAGCTTGGTCGTGTTTCTGGAGTTATAc                       <  2:2937580/150‑1 (MQ=255)
                                                                                                                            aaTACTGCGCGCGCGGTTCCAGCTTTTGGGTATAACCCGGAGCCAGCATTTCCATATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCGGCATTACGCGGTTAACGGATTCGTCCAGCTTGGTCGTGTTTCTGGAGTTATAcc                      <  1:4273520/150‑1 (MQ=255)
                                                                                                                            aaTACTGCGCGCGCGGTTCCAGCTTTTGGGTATAACCCGGAGCCAGCATTTCCATATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCGGCATTACGCGGTTAACGGATTCGTCCAGCTTGGTCGTGTTTCTGGAGTTATAcc                      >  1:1915232/1‑150 (MQ=255)
                                                                                                                               aCTGCGCGCGCGGTTCCAGCTTTTGGGTATAACCCGGAGCCAGCATTTCCATATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCGGCATTACGCGGTTAACGGATTCGTCCAGCTTGGTCGTGTTTCTGGAGTTATACCAGt                   <  1:4060038/150‑1 (MQ=255)
                                                                                                                                 tGCGCGCGCGGTTCCAGCTTTTGGGTATAACCCGGAGCCAGCATTTCCATATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCGGCATTACGCGGTTAACGGATTCGTCCAGCTTGGTCGTGTTTCTGGAGTTATACCAGTCa                 >  2:1407109/1‑150 (MQ=255)
                                                                                                                                  gcgcgcgcGGTTCCAGCTTTTGGGTATAACCCGGAGCCAGCATTTCCATATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCGGCATTACGCGGTTAACGGATTCGTCCAGCTTGGTCGTGTTTCTGGAGTTATACCAGTCaa                >  1:1363583/1‑150 (MQ=255)
                                                                                                                                     cgcgcgGTTCCAGCTTTTGGGTATAACCCGGAGCCAGCATTTCCATATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCGGCATTACGCGGTTAACGGATTCGTCCAGCTTGGTCGTGTTTCTGGAGTTATACCAGTCAAGAt             <  1:4103510/150‑1 (MQ=255)
                                                                                                                                         cgGTTCCAGCTTTTGGGTATAACCCGGAGCCAGCATTTCCATATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCGGCATTACGCGGTTAACGGATTCGTCCAGCTTGGTCGTGTTTCTGGAGTTATACCAGTCAAGATTGGt         >  1:2331310/1‑150 (MQ=255)
                                                                                                                                           gTTCCAGCGTTTGGGTATAACCCGGAGCCAGCATTTCCATATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCGGCATTACGCGGTTAACGGATTCGTCCAGCTTGGTCGTGTTTCTGGAGTTATACCAGTCAAGATTGGttt       >  2:4244029/1‑150 (MQ=255)
                                                                                                                                              ccAGCTTTTGGGTATAACCCGGAGCCAGCATTTCCATATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCGGCATTACGCGGTTAACGGATTCGTCCAGCTTGGTCGTGTTTCTGGAGTTATACCAGTCAAGATTGGTTTGCt    <  2:2566744/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
TTAGCGGAGGCAATACGGTCAAGACCGCCGTAAGTCCGGTCCCGGAACAGGCCAGAGTAGTCAGATTGCAGCAGAGACGAGTCGTAGTTATAGATGTCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCGTTTGGGTATAACCCGGAGCCAGCATTTCCATATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCGGCATTACGCGGTTAACGGATTCGTCCAGCTTGGTCGTGTTTCTGGAGTTATACCAGTCAAGATTGGTTTGCTGA  >  CP009273/55443‑55736

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑