Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 602,045 C→A 12.6% L265L (CTG→CTT)  ybdK ← weak gamma‑glutamyl:cysteine ligase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273602,0450CA12.6% 1139.1 / 96.4 332L265L (CTG→CTT) ybdKweak gamma‑glutamyl:cysteine ligase
Reads supporting (aligned to +/- strand):  ref base C (146/144);  new base A (15/27);  total (161/171)
Fisher's exact test for biased strand distribution p-value = 9.80e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 336 total aligned reads displayed.

AGCAATCGCAAGGTATCTTCCGTTAGCGGTCGACGATCTCCAGTGTGCGGATCGGTGATGACGCCTTCAAGCCCATAGCGACAGGCCTGGAAACGGTTGAATTTATACAGCAGGTAATCTTTTTCCTGATGTTTAAACGGGCGTTCCGTCAGTAACCAGTGGGCGGTAGCCTGAATTAATCCCGCCATATTTACTGCGTGGCTAAGGGTTAACGGGGTATCCATCACCCGAACCTCCACCGTGCCAAAATGAGGACTGGGGCGAATATCCCAGTGCAGATCTTTAATGCTGTCGATCAT  >  CP009273/601896‑602194
                                                                                                                                                     |                                                                                                                                                     
aGCAATCGCAAGGTATCTTCCGTTAGCGGTCGACGATCTCCAGTGTGCGGATCGGTGATGACGCCTTCAAGCCCATAGCGACAGGCCTGGAAACGGTTGAATTTATACAGCAGGTAATCTTTTTCCTGATGTTTAAACGGGCGTTCCGTc                                                                                                                                                       <  2:1739417/150‑1 (MQ=255)
  cAATCGCAAGGTATCTTCCGTTAGCGGTCGACGATCTCCAGTGTGCGGATCGGTGATGACGCCTTCAAGCCCATAGCGACAGGCCTGGAAACGGTTGAATTTATACAGCAGGTAATCTTTTTCCTGATGTTTAAACGGGCGTTCCGTCAg                                                                                                                                                     >  2:2544807/1‑150 (MQ=255)
       gCAAGGTATCTTCCGTTAGCGGTCGACGATCTCCAGTGTGCGGATCGGTGATGACGCCTTCAAGCCCATAGCGACAGGCCTGGAAACGGTTGAATTTATACAGCAGGTAATCTTTTTCCTGATGTTTAAACGGGCGTTCCGTCAGTAAcc                                                                                                                                                >  2:463805/1‑150 (MQ=255)
        cAAGGTATCTTCCGTTAGCGGTCGACGATCTCCAGTGTGCGGATCGGTGATGACGCCTTCAAGCCCATAGCGACAGGCCTGGAAACGGTTGAATTTATACAGCAGGTAATCTTTTTCCTGATGTTTAAACGGGCGTTCCGTCAGTAACCa                                                                                                                                               <  1:2314238/150‑1 (MQ=255)
        cAAGGTATCTTCCGTTAGCGGTCGACGATCTCCAGTGTGCGGATCGGTGATGACGCCTTCAAGCCCATAGCGACAGGCCTGGAAACGGTTGAATTTATACAGCAGGTAATCTTTTTCCTGATGTTTAAACGGGCGTTCCGTCAGTAACCa                                                                                                                                               <  1:5051152/150‑1 (MQ=255)
        cAAGGTATCTTCCGTTAGCGGTCGACGATCTCAAGTGTGCGGATCGGTGATGACGCCTTCAAGCCCATAGCGACAGGCCTGGAAACGGTTGAATTTATACAGCAGGTAATCTGTTTCCTGATGTTTAAACGGGCGTTCCGTCAGTAACCa                                                                                                                                               >  1:4585691/1‑150 (MQ=255)
          aGGTATCTTCCGTTAGCGGTCGACGATCTCCAGTGTGCGGATCGGTGATGACGCCTTCAAGCCCATAGCGACAGGCCTGGAAACGGTTGAATTTATACAGCAGGTAATCTTTTTCCTGATGTTTAAACGGGCGTTCCGTCAGTAACCAGt                                                                                                                                             <  2:3159138/150‑1 (MQ=255)
          aGGTATCTTCCGTTAGCGGTCGACGATCTCCAGTGTGCGGATCGGTGATGACGCCTTCAAGCCCATAGCGACAGGCCTGGAAACGGTTGAATTTATACAGCAGGTAATCTTTTTCCTGATGTTTAAACGGGCGTTCCGTCAGTAACCAGt                                                                                                                                             >  2:2439908/1‑150 (MQ=255)
          aGGTATCTTCCGTTAGCGGTCGACGATCTCCAGTGTGCGGATCGGTGATGACGCCTTCAAGCCCATAGCGACAGGCCTGGAAACGGTTGAATTTATACAGCAGGTAATCTTTTTCCTGATGTTTAAACGGGCGTTCCGTAAGTAACCAGt                                                                                                                                             <  1:2410929/150‑1 (MQ=255)
           ggTATCTTCCGTTAGCGGTCGACGATCTCCAGTGTGCGGATCGGTGATGACGCCTTCAAGCCCATAGCGACAGGCCTGGAAACGGTTGAATTTATACAGCAGGTAATCTTTTTCCTGATGTTTAAACGGGCGTTCCGTCAGTAACCAGTg                                                                                                                                            >  2:1687591/1‑150 (MQ=255)
            gTATCTTCCGTTAGCGGTCGACGATCTCCAGTGTGCGGATCGGTGATGACGCCTTCAAGCCCATAGCGACAGGCCTGGAAACGGTTGAATTTATACAGCAGGTAATCTTTTTCCTGATGTTTAAACGGGCGTTCCGTCAGTAACCAGTgg                                                                                                                                           <  2:1194135/150‑1 (MQ=255)
                cTTCCGTTAGCGGTCGACGATCTCCAGTGTGCGGATCGGTGATGACGCCTTCAAGCCCATAGCGACAGGCCTGGAAACGGTTGAATTTATACAGCAGGTAATCTTTTTCCTGATGTTTAAACGGGCGTTCCGTAAGTAACCAGTGGGCgg                                                                                                                                       <  1:425670/150‑1 (MQ=255)
                    cGTTAGCGGTCGACGATCTCCAGTGTGCGGATCGGTGATGACGCCTTCAAGCCCATAGCGACAGGCCTGGAAACGGTTGAATTTATACAGCAGGTAATCTTTTTCCTGATGTTTAAACGGGCGTTCCGTCAGTAACCAGTGGGCGGTAGc                                                                                                                                   <  1:958989/150‑1 (MQ=255)
                    cGTTAGCGGTCGACGATCTCCAGTGTGCGGATCGGTGATGACGCCTTCAAGCCCATAGCGACAGGCCTGGAAACGGTTGAATTTATACAGCAGGTAATCTTTTTCCTGATGTTTAAACGGGCGTTCCGTAAGTAACCAGTGGGCGGTAGc                                                                                                                                   >  2:3879281/1‑150 (MQ=255)
                           ggTCGACGATCTCCAGTGTGCGGATCGGTGATGACGCCTTCAAGCCCATAGCGACAGGCCTGGAAACGGTTGAATTTATACAGCAGGTAATCTTTTTCCTGATGTTTAAACGGGCGTTCCGTCAGTAACCAGTGGGCGGTAGCCTGAAtt                                                                                                                            <  1:3017004/150‑1 (MQ=255)
                             tCGACGATCTCCAGTGTGCGGATCGGTGATGACGCCTTCAAGCCCATAGCGACAGGCCTGGAAACGGTTGAATTTATACAGCAGGTAATCTTTTTCCTGATGTTTAAACGGGCGTTCCGTCAGTAACCAGTGGGCGGTAGCCTGAATTaa                                                                                                                          <  2:1310240/150‑1 (MQ=255)
                               gacgaTCTCCAGTGTGCGGATCGGTGATGACGCCTTCAAGCCCATAGCGACAGGCCTGGAAACGGTTGAATTTATACAGCAGGTAATCTTTTTCCTGATGTTTAAACGGGCGTTCCGTCAGTAACCAGTGGGCGGTAGCCTGAATTAATc                                                                                                                        >  1:2544819/1‑150 (MQ=255)
                                  gaTCTCCAGTGTGCGGATCGGTGATGACGCCTTCAAGCCCATAGCGACAGGCCTGGAAACGGTTGAATTTATACAGCAGGTAATCTTTTTCCTGATGTTTAAACGGGCGTTCCGTCAGTAACCAGTGGGCGGTAGCCTGAATTAATCCCg                                                                                                                     >  2:1069564/1‑150 (MQ=255)
                                  gaTCTCCAGTGTGCGGATCGGTGATGACGCCTTCAAGCCCATAGCGACAGGCCTGGAAACGGTTGAATTTATACAGCAGGTAATCTTTTTCCTGATGTTTAAACGGGCGTTCCGTCAGTAACCAGTGGGCGGTAGCCTGAATTAATCCCg                                                                                                                     >  1:431974/1‑150 (MQ=255)
                                      tcCAGTGTGCGGATCGGTGATGACGCCTTCAAGCCCATAGCGACAGGCCTGGAAACGGTTGAATTTATACAGCAGGTAATCTTTTTCCTGATGTTTAAACGGGCGTTCCGTCAGTAACCAGTGGGCGGTAGCCTGAATTAATCCCGCCat                                                                                                                 <  1:4109492/150‑1 (MQ=255)
                                        cAGTGTGCGGATCGGTGATGACGCCTTCAAGCCCATAGCGACAGGCCTGGAAACGGTTGAATTTATACAGCAGGTAATCTTTTTCCTGATGTTTAAACGGGCGTTCCGTCAGTAACCAGTGGGCGGTAGCCTGAATTAATCCCGCCatat                                                                                                               >  2:3175744/1‑150 (MQ=255)
                                        cAGTGTGCGGATCGGTGATGACGCCTTCAAGCCCATAGCGACAGGCCTGGAAACGGTTGAATTTATACAGCAGGTAATCTTTTTCCTGATGTTTAAACGGGCGTTCCGTCAGTAACCAGTGGGCGGTAGCCTGAATTAATCCCGCCatat                                                                                                               <  2:3491776/150‑1 (MQ=255)
                                          gtgtGCGGATCGGTGATGACGCCTTCAAGCCCATAGCGACAGGCCTGGAAACGGTTGAATTTATACAGCAGGTAATCTTTTTCCTGATGTTTAAACGGGCGTTCCGTCAGTAACCAGTGGGCGGTAGCCTGAATTAATCCCGCCATAttt                                                                                                             <  2:2696499/150‑1 (MQ=255)
                                           tgtgCGGATCGGTGATGACGCCTTCAAGCCCATAGCGACAGGCCTGGAAACGGTTGAATTTATACAGCAGGTAATCTTTTTCCTGATGTTTAAACGGGCGTTCCGTCAGTAACCAGTGGGCGGTAGCCTGAATTAATCCCGCCATATTTa                                                                                                            >  2:2967842/1‑150 (MQ=255)
                                            gtgCGGATCGGTGATGACGCCTTCAAGCCCATAGCGACAGGCCTGGAAACGGTTGAATTTATACAGCAGGTAATCTTTTTCCTGATGTTTAAACGGGCGTTCCGTCAGTAACCAGTGGGCGGTAGCCTGAATTAATCCCGCCATATTTAc                                                                                                           >  2:1493981/1‑150 (MQ=255)
                                               cGGATCGGTGATGACGCCTTCAAGCCCATAGCGACAGGCCTGGAAACGGTTGAATTTATACAGCAGGTAATCTTTTTCCTGATGTTTAAACGGGCGTTCCGTCAGTAACCAGTGGGCGGTAGCCTGAATTAATCCCGCCATATTTACTGc                                                                                                        <  1:3056247/150‑1 (MQ=255)
                                                ggATCGGTGATGACGCCTTCAAGCCCATCGCGACAGGCCTGGAAACGGTTGAATTTATACAGCAGGTAATCTTTTTCCTGATGTTTAAACGGGCGTTCCGTCAGTAACCAGTGGGCGGTAGCCTGAATTAATCCCGCCATATTTACTGCg                                                                                                       <  1:2027380/150‑1 (MQ=255)
                                                  aTCGGTGATGACGCCTTCAAGCCCATAGCGACAGGCCTGGAAACGGTTGAATTTATACAGCAGGTAATCTTTTTCCTGATGTTTAAACGGGCGTTCCGTCAGTAACCAGTGGGCGGTAGCCTGAATTAATCCCGCCATATTTACTGCGTg                                                                                                     <  2:5046975/150‑1 (MQ=255)
                                                    cGGTGATGACGCCTTCAAGCCCATAGCGACAGGCCTGGAAACGGTTGAATTTATACAGCAGGTAATCTTTTTCCTGATGTTTAAACGGGCGTTCCGTCAGTAACCAGTGGGCGGTAGCCTGAATTAATCCCGCCATATTTACTGCGTGGc                                                                                                   >  1:4604168/1‑150 (MQ=255)
                                                     ggTGATGACGCCTTCAAGCCCATAGCGACAGGCCTGGAAACGGTTGAATTTATACAGCAGGTAATCTTTTTCCTGATGTTTAAACGGGCGTTCCGTCAGTAACCAGTGGGCGGTAGCCTGAATTAATCCCGCCATATTTACTGCGTGGCt                                                                                                  <  2:766137/150‑1 (MQ=255)
                                                     ggTGATGACGCCTTCAAGCCCATAGCGACAGGCCTGGAAACGGTTGAATTTATACAGCAGGTAATCTTTTTCCTGATGTTTAAACGGGCGTTCCGTCAGTAACCAGTGGGCGGTAGCCTGAATTAATCCCGCCATATTTACTGCGTGGCt                                                                                                  >  2:2810521/1‑150 (MQ=255)
                                                      gTGATGACGCCTTCAAGCCCATAGCGACAGGCCTGGAAACGGTTGAATTTATACAGCAGGTAATCTTTTTCCTGATGTTTAAACGGGCGTTCCGTCAGTAACCAGTGGGCGGTAGCCTGAATTAATCCCGCCATATTTACTGCGTGGCTa                                                                                                 <  2:2277656/150‑1 (MQ=255)
                                                      gTGATGACGCCTTCAAGCCCATAGCGACAGGCCTGGAAACGGTTGAATTTATACAGCAGGTAATCTTTTTCCTGATGTTTAAACGGGCGTTCCGTAAGTAACCAGTGGGCGGTAGCCTGAATTAATCCCGCCATATTTACTGCGTGGCTa                                                                                                 <  1:1075593/150‑1 (MQ=255)
                                                        gatgaCGCCTTCAAGCCCATAGCGACAGGCCTGGAAACGGTTGAATTTATACAGCAGGTAATCTTTTTCCTGATGTTTAAACGGGCGTTCCGTCAGTAACCAGTGGGCGGTAGCCTGAATTAATCCCGCCATATTTACTGCGTGGCTAAg                                                                                               >  1:2767345/1‑150 (MQ=255)
                                                         atgaCGCCTTCAAGCCCATAGCGACAGGCCTGGAAACGGTTGAATTTATACAGCAGGTAATCTTTTTCCTGATGTTTAAACGGGCGTTCCGTCAGTAACCAGTGGGCGGTAGCCTGAATTAATCCCGCCATATTTACTGCGTGGCTAAgg                                                                                              <  1:4824843/150‑1 (MQ=255)
                                                         atgaCGCCTTCAAGCCCATAGCGACAGGCCTGGAAACGGTTGAATTTATACAGCAGGTAATCTTTTTCCTGATGTTTAAACGGGCGTTCCGTCAGTAACCAGTGGGCGGTAGCCTGAATTAATCCCGCCATATTTACTGCGTGGCTAAgg                                                                                              <  2:3673715/150‑1 (MQ=255)
                                                           gaCGCCTTCAAGCCCATAGCGACAGGCCTGGAAACGGTTGAATTTATACAGCAGGTAATCTTTTTCCTGATTTTTAAACGGGCGTTCCGTAAGTAACCAGTGGGCGGTAGCCTGAATTAATCCCGCCATATTTACTGCGTGGCTAAGGGt                                                                                            <  2:3690211/150‑1 (MQ=255)
                                                              gCCTTCAAGCCCATAGCGACAGGCCTGGAAACGGTTGAATTTATACAGCAGGTAATCTTTTTCCTGATGTTTAAACGGGCGTTCCGTCAGTAACCAGTGGGCGGTAGCCTGAATTAATCCCGCCATATTTACTGCGTGGCTAAGGGTTaa                                                                                         <  2:3772524/150‑1 (MQ=255)
                                                               ccTTCAAGCCCATAGCGACAGGCCTGGAAACGGTTGAATTTATACAGCAGGTAATCTTTTTCCTGATGTTTAAACGGGCGTTCCGTCAGTAACCAGTGGGCGGTAGCCTGAATTAATCCCGCCATATTTACTGCGTGGCTAAGGGTTAAc                                                                                        <  2:4045840/150‑1 (MQ=255)
                                                               ccTTCAAGCCCAGAGCGACAGGCCTGGAAACGGTTGAATTTATACAGCAGGTAATCTTTTTCCTGATGTTTAAACGGGCGTTCCGTCAGTAACCAGTGGGCGGTAGCCTGAATTAATCCCGCCATATTTACTGCGTGGCTAAGGGTTAAc                                                                                        <  2:4133648/150‑1 (MQ=255)
                                                                cTTCAAGCCCATAGCGACAGGCCTGGAAACGGTTGAATTTATACAGCAGGTAATCTTTTTCCTGATGTTTAAACGGGCGTTCCGTAAGTAACCAGTGGGCGGTAGCCTGAATTAATCCCGCCATATTTACTGCGTGGCTAAGGGTTAACg                                                                                       <  2:3984884/150‑1 (MQ=255)
                                                                cTTCAAGCCCAAAGCGACAGGCCTGGAAACGGTTGAATTTATACCGCAGGTAATCATTTTCCTGATGTTTAAACGGGCGTTCCGTCAGTAACCAGTGGGCGGTAGCCTGAATTAATCCCGCCAAATTTACTGCGAGGCTAAGGGTTAACg                                                                                       >  2:4976131/1‑150 (MQ=255)
                                                                   cAAGCCCATAGCGACAGGCCTGGAAACGGTTGAATTTATACAGCAGGTAATCTTTTTCCTGATGTTTAAACGGGCGTTCCGTAAGTAACCAGTGGGCGGTAGCCTGAATTAATCCCGCCATATTTACTGCGTGGCTAAGGGTTAACgggg                                                                                    <  1:4766358/150‑1 (MQ=255)
                                                                     aGCCCATAGCGACAGGCCTGGAAACGGTTGAATTTATACAGCAGGTAATCTTTTTCCTGATGTTTAAACGGGCGTTCCGTCAGTAACCAGTGGGCGGTAGCCTGAATTAATCCCGCCATATTTACTGCGTGGCTAAGGGTTAACGGGGTa                                                                                  <  1:4664641/150‑1 (MQ=255)
                                                                        ccATAGCGACAGGCCTGGAAACGGTTGAATTTATACAGCAGGTAATCTTTTTCCTGATGTTTAAACGGGCGTTCCGTCAGTAACCAGTGGGCGGTAGCCTGAATTAATCCCGCCATATTTACTGCGTGGCTAAGGGTTAACGGGGTATcc                                                                               >  2:657645/1‑150 (MQ=255)
                                                                          aTAGCGACAGGCCTGGAAACGGTTGAATTTATACAGCAGGTAATCTTTTTCCTGATGTTTAAACGGGCGTTCCGTCAGTAACCAGTGGGCGGTAGCCTGAATTAATCCCGCCATATTTACTGCGTGGCTAAGGGTTAACGGGGTATCCAt                                                                             <  2:4236660/150‑1 (MQ=255)
                                                                            aGCGACAGGCCTGGAAACGGTTGAATTTATACAGCAGGTAATCTTTTTCCTGATGTTTAAACGGGCGTTCCGTCAGTAACCAGTGGGCGGTAGCCTGAATTAATCCCGCCATATTTACTGCGTGGCTAAGGGTTAACGGGGTATCCATCa                                                                           <  2:4758838/150‑1 (MQ=255)
                                                                            aGCGACAGGCCTGGAAACGGTTGAATTTATACAGCAGGTAATCTTTTTCCTGATGTTTAAACGGGCGTTCCGTCAGTAACCAGTGGGCGGTAGCCTGAATTAATCCCGCCATATTTACTGCGTGGCTAAGGGTTAACGGGGTATCCATCa                                                                           <  1:1302857/150‑1 (MQ=255)
                                                                            aGCGACAGGCCTGGAAACGGTTGAATTTATACAGCAGGTAATCTTTTTCCTGATGTTTAAACGGGCGTTCCGTAAGTAACCAGTGGGCGGTAGCCTGAATTAATCCCGCCATATTTACTGCGTGGCTAAGGGTTAACGGGGTATCCATCa                                                                           <  1:4519176/150‑1 (MQ=255)
                                                                             gCGACAGGCCTGGAAACGGTTGAATTTATACAGCAGGTAATCTTTTTCCTGATGTTTAAACGGGCGTTCCGTCAGTAACCAGTGGGCGGTAGCCTGAATTAATCCCGCCATATTTACTGCGTGGCTAAGGGTTAACGGGGTATCCATCAc                                                                          <  1:41799/150‑1 (MQ=255)
                                                                              cGACAGGCCTGGAAACGGTTGAATTTATACAGCAGGTAATCTTTTTCCTGATGTTTAAACGGGCGTTCCGTCAGTAACCAGTGGGCGGTAGCCTGAATTAATCCCGCCATATTTACTGCGTGGCTAAGGGTTAACGGGGTATCCATCAcc                                                                         >  2:3798814/1‑150 (MQ=255)
                                                                               gACAGGCCTGGAAACGGTTGAATTTATACAGCAGGTAATCTTTTTCCTGATGTTTAAACGGGCGTTCCGTCAGTAACCAGTGGGCGGTAGCCTGAATTAATCCCGCCATATTTACTGCGTGGCTAAGGGTTAACGGGGTATCCATCAccc                                                                        <  2:4902455/150‑1 (MQ=255)
                                                                                aCAGGCCTGGAAACGGTTGAATTTATACAGCAGGTAATCTTTTTCCTGATGTTTAAACGGGCGTTCCGTCAGTAACCAGTGGGCGGTAGCCTGAATTAATCCCGCCATATTTACTGCGTGGCTAAGGGTTAACGGGGTATCCATCACCCg                                                                       <  1:3356699/150‑1 (MQ=255)
                                                                                 cAGGCCTGGAAACGGTTGAATTTATACAGCAGGTAATCTTTTTCCTGATGTTTAAACGGGCGTTCCGTCAGTAACCAGTGGGCGGTAGCCTGAATTAATCCCGCCATATTTACTGCGTGGCTAAGGGTTAACGGGGTATCCATCACCCGa                                                                      >  1:4023223/1‑150 (MQ=255)
                                                                                 cAGGCCTGGAAACGGTTGAATTTATACAGCAGGTAATCTTTTTCCTGATGTTTAAACGGGCGTTCCGTCAGTAACCAGTGGGCGGTAGCCTGAATTAATCCCGCCATATTTACTGCGTGGCTAAGGGTTAACGGGGTATCCATCACCCGa                                                                      >  1:1645457/1‑150 (MQ=255)
                                                                                  aGGCCTGGAAACGGTTGAATTTATACAGCAGGTAATCTTTTTCCTGATGTTTAAACGGGCGTTCCGTAAGTAACCAGTGGGCGGTAGCCTGAATTAATCCCGCCAAATTTACTGCGTGGCTAAGGGTTAACGGGGTATCCATCACCCGaa                                                                     >  1:1722119/1‑150 (MQ=255)
                                                                                      cTGGAAACGGTTGAATTTATACAGCAGGTAATCTTTTTCCTGATGTTTAAACGGGCGTTCCGTAAGTAACCAGTGGGCGGTAGCCTGAATTAATCCCGCCATATTTACTGCGTGGCTAAGGGTTAACGGGGTATCCATCACCCGAACCTc                                                                 >  2:852284/1‑150 (MQ=255)
                                                                                       tGGAAACGGTTGAATTTATACAGCAGGTAATCTTTTTCCTGATGTTTAAACGGGCGTTCCGTCAGTAACCAGTGGGCGGTAGCCTGAATTAATCCCGCCATATTTACTGCGTGGCTAAGGGTTAACGGGGTATCCATCACCCGAACCTcc                                                                <  1:892296/150‑1 (MQ=255)
                                                                                       tGGAAACGGTTGAATTTATACAGCAGGTAATCTTTTTCCTGATGTTTAAACGGGCGTTCCGTCAGTAACCAGTGGGCGGTAGCCTGAATTAATCCCGCCATATTTACTGCGTGGCTAAGGGTTAACGGGGTATCCATCACCCGAACCTcc                                                                <  2:90379/150‑1 (MQ=255)
                                                                                         gAAACGGTTGAATTTATACAGCAGGTAATCTTTTTCCTGATGTTTAAACGGGCGTTCCGTCAGTAACCAGTGGGCGGTAGCCTGAATTAATCCCGCCATATTTACTGCGTGGCTAAGGGTTAACGGGGTATCCATCACCCGAACCTCCAc                                                              >  1:4203614/1‑150 (MQ=255)
                                                                                          aaaCGGTTGAATTTATACAGCAGGTAATCTTTTTCCTGATGTTTAAACGGGCGTTCCGTCAGTAACCAGTGGGCGGTAGCCTGAATTAATCCCGCCATATTTACTGCGTGGCTAAGGGTTAACGGGGTATCCATCACCCGAACCTCCAcc                                                             >  2:1140660/1‑150 (MQ=255)
                                                                                          aaaCGGTTGAATTTATACAGCAGGTAATCTTTTTCCTGATGTTTAAACGGGCGTTCCGTCAGTAACCAGTGGGCGGTAGCCTGAATTAATCCCGCCATATTTACTGCGTGGCTAAGGGTTAACGGGGTATCCATCACCCGAACCTCCAcc                                                             >  2:4185080/1‑150 (MQ=255)
                                                                                            aCGGTTGAATTTATACAGCAGGTAATCTTTTTCCTGATGTTTAAACGGGCGTTCCGTCAGTAACCAGTGGGCGGTAGCCTGAATTAATCCCGCCATATTTACTGCGTGGCTAAGGGTTAACGGGGTATCCATCACCCGAACCTCCACCGt                                                           <  2:3321299/150‑1 (MQ=255)
                                                                                             cGGTTGAATTTATACAGCAGGTAATCTTTTTCCTGATGTTTAAACGGGCGTTCCGTCAGTAACCAGTGGGCGGTAGCCTGAATTAATCCCGCCATATTTACTGCGTGGCTAAGGGTTAACGGGGTATCCATCACCCGAACCTCCACCGTg                                                          >  2:2879857/1‑150 (MQ=255)
                                                                                              ggTTGAATTTATACAGCAGGTAATCTTTTTCCTGATGTTTAAACGGGCGTTCCGTCAGTAACCAGTGGGCGGTAGCCTGAATTAATCCCGCCATATTTACTGCGTGGCTAAGGGTTAACGGGGTATCCATCACCCGAACCTCCACCGTGc                                                         >  2:1823730/1‑150 (MQ=255)
                                                                                               gTTGAATTTATACAGCAGGTAATCTTTTTCCTGATGTTTAAACGGGCGTTCCGTCAGTAACCAGTGGGCGGTAGCCTGAATTAATCCCGCCATATTTACTGCGTGGCTAAGGGTTAACGGGGTATCCATCACCCGAACCTCCACCGTGcc                                                        >  2:2182032/1‑150 (MQ=255)
                                                                                               gTTGAATTTATACAGCAGGTAATCTTTTTCCTGATGTTTAAACGGGCGTTCCGTCAGTAACCAGTGGGCGGTAGCCTGAATTAATCCCGCCATATTTACTGCGTGGCTAAGGGTTAACGGGGTATCCATCACCCGAACCTCCACCGTGcc                                                        >  2:877999/1‑150 (MQ=255)
                                                                                                   aaTTTATACAGCAGGTAATCTTTTTCCTGATGTTTAAACGGGCGTTCCGTCAGTAACCAGTGGGCGGTAGCCTGAATTAATCCCGCCATATTTACTGCGTGGCTAAGGGTTAACGGGGTATCCATCACCCGAACCTCCACCGTGCCaaaa                                                    >  2:2653691/1‑150 (MQ=255)
                                                                                                       tataCAGCAGGTAATCTTTTTCCTGATGTTTAAACGGGCGTTCCGTCAGTAACCAGTGGGCGGTAGCCTGAATTAATCCCGCCATATTTACTGCGTGGCTAAGGGTTAACGGGGTATCCATCACCCGAACCTCCACCGTGCCAAAATGAg                                                <  2:1397490/150‑1 (MQ=255)
                                                                                                         taCAGCAGGTAATCTTTTTCCTGATGTTTAAACGGGCGTTCCGTCAGTAACCAGTGGGCGGTAGCCTGAATTAATCCCGCCATATTTACTGCGTGGCTAAGGGTTAACGGGGTATCCATCACCCGAACCTCCACCGTGCCAAAATGAGGa                                              <  1:2086755/150‑1 (MQ=255)
                                                                                                          aCAGCAGGTAATCTTTTTCCTGATGTTTAAACGGGCGTTCCGTCAGTAACCAGTGGGCGGTAGCCTGAATTAATCCCGCCATATTTACTGCGTGGCTAAGGGTTAACGGGGTATCCATCACCCGAACCTCCACCGTGCCAAAATGAGGAc                                             >  2:910597/1‑150 (MQ=255)
                                                                                                           cagcagGTAATCTTTTTCCTGATGTTTAAACGGGCGTTCCGTCAGTAACCAGTGGGCGGTAGCCTGAATTAATCCCGCCATATTTACTGCGTGGCTAAGGGTTAACGGGGTATCCATCACCCGAACCTCCACCGTGCCAAAATGAGGACt                                            >  1:1866847/1‑150 (MQ=255)
                                                                                                           cagcagGTAATCTTTTTCCTGATGTTTAAACGGGCGTTCCGTCAGTAACCAGTGGGCGGTAGCCTGAATTAATCCCGCCATATTTACTGCGTGGCTAAGGGTTAACGGGGTATCCATCACCCGAACCTCCACCGTGCCAAAATGAGGACt                                            <  1:2967842/150‑1 (MQ=255)
                                                                                                            agcagGTAATCTTTTTCCTGATTTTTAAACGGGCGTTCCGTAAGTAACCAGTGGGCGGTAGCCTGAATTAATCCCGCCATATTTACTGCGTGGCTAAGGGTTAACGGGGTATCCATCACCCGAACCTCCACCGTGCCAAAATGAGGACTg                                           <  1:2709959/150‑1 (MQ=255)
                                                                                                                ggTAATCTTTTTCCTGATGTTTAAACGGGCGTTCCGTAAGTAACCAGTGGGCGGTAGCCTGAATTAATCCCGCCATATTTACTGCGTGGCTAAGGGTTAACGGGGTATCCATCACCCGAACCTCCACCGTGCCAAAATGAGGACTGGGGc                                       >  1:642563/1‑150 (MQ=255)
                                                                                                                    aTCTTTTTCCTGATGTTTAAACGGGCGTTCCGTCAGTAACCAGTGGGCGGTAGCCTGAATTAATCCCGCCATATTTACTGCGTGGCTAAGGGTTAACGGGGTATCCATCACCCGAACCTCCACCGTGCCAAAATGAGGACTGGGGCGAat                                   <  1:963232/150‑1 (MQ=255)
                                                                                                                    aTCTTTTTCCTGATGTTTAAACGGGCGTTCCGTCAGTAACCAGTGGGCGGTAGCCTGAATTAATCCCGCCATATTTACTGCGTGGCTAAGGGTTAACGGGGTATCCATCACCCGAACCTCCACCGTGCCAAAATGAGGACTGGGGCGAat                                   <  2:5055953/150‑1 (MQ=255)
                                                                                                                       tttttCCTGATGTTTAAACGGGCGTTCCGTCAGTAACCAGTGGGCGGTAGCCTGAATTAATCCCGCCATATTTACTGCGTGGCTAAGGGTTAACGGGGTATCCATCACCCGAACCTCCACCGTGCCAAAATGAGGACTGGGGCGAATATc                                <  2:1853253/150‑1 (MQ=255)
                                                                                                                           tCCTGATGTTTAAACGGGCGTTCCGTCAGTAACCAGTGGGCGGTAGCCTGAATTAATCCCGCCATATTTACTGCGTGGCTAAGGGTTAACGGGGTATCCATCACCCGAACCTCCACCGTGCCAAAATGAGGACTGGGGCGAATATCCCAg                            >  2:2089275/1‑150 (MQ=255)
                                                                                                                           tCCTGATGTTTAAACGGGCGTTCCGTCAGTAACCAGTGGGCGGTAGCCTGAATTAATCCCGCCATATTTACTGCGTGGCTAAGGGTTAACGGGGTATCCATCACCCGAACCTCCACCGTGCCAAAATGAGGACTGGGGCGAATATCCCAg                            <  1:1493981/150‑1 (MQ=255)
                                                                                                                           tCCTGATGTTTAAACGGGCGTTCCGTCAGTAACCAGTGGGCGGTAGCCTGAATTAATCCCGCCATATTTACTGCGTGGCTAAGGGTTAACGGGGTATCCATCACCCGAACCTCCACCGTGCCAAAATGAGGACTGGGGCGAATATCCCAg                            >  1:4957806/1‑150 (MQ=255)
                                                                                                                             cTGATGTTTAAACGGGCGTTCCGTCAGTAACCAGTGGGCGGTAGCCTGAATTAATCCCGCCATATTTACTGCGTGGCTAAGGGTTAACGGGGTATCCATCACCCGAACCTCCACCGTGCCAAAATGAGGACTGGGGCGAATATCCCAGTg                          <  2:2075445/150‑1 (MQ=255)
                                                                                                                              tGATGTTTAAACGGGCGTTCCGTCAGTAACCAGTGGGCGGTAGCCTGAATTAATCCCGCCATATTTACTGCGTGGCTAAGGGTTAACGGGGTATCCATCACCCGAACCTCCACCGTGCCAAAATGAGGACTGGGGCGAATATCCCAGTGc                         >  1:762172/1‑150 (MQ=255)
                                                                                                                              tGATGTTTAAACGGGCGTTCCGTCAGTAACCAGTGGGCGGTAGCCTGAATTAATCCCGCCATATTTACTGCGTGGCTAAGGGTTAACGGGGTATCCATCACCCGAACCTCCACCGTGCCAAAATGAGGACTGGGGCGAATATCCCAGTGc                         <  1:4290786/150‑1 (MQ=255)
                                                                                                                               gATGTTTAAACGGGCGTTCCGTCAGTAACCAGTGGGCGGTAGCCTGAATTAATCCCGCCATATTTACTGCGTGGCTAAGGGTTAACGGGGTATCCATCACCCGAACCTCCACCGTGCCAAAATGAGGACTGGGGCGAATATCCCAGTGCa                        >  1:3726227/1‑150 (MQ=255)
                                                                                                                                 tGTTTAAACGGGCGTTCCGTCAGTAACCAGTGGGCGGTAGCCTGAATTAATCCCGCCATATTTACTGCGTGGCTAAGGGTTAACGGGGTATCCATCACCCGAACCTCCACCGTGCCAAAATGAGGACTGGGGCGAATATCCCAGTGCAGa                      <  2:1548063/150‑1 (MQ=255)
                                                                                                                                    ttAAACGGGCGTTCCGTCAGTAACCAGTGGGCGGTAGCCTGAATTAATCCCGCCATATTTACTGCGTGGCTAAGGGTTAACGGGGTATCCATTACCCGAACCTCCACCGTGCCAAAATGAGGACTGGGGCGAATATCCCAGTGCAGATCt                   <  2:1973945/150‑1 (MQ=255)
                                                                                                                                       aaCGGGCGTTCCGTCAGTAACCAGTGGGCGGTAGCCTGAATTAATCCCGCCATATTTACTGCGTGGCTAAGGGTTAACGGGGTATCCATCACCCGAACCTCCACCGTGCCAAAATGAGGACTGGGGCGAATATCCCAGTGCAGATCTTTa                >  1:350955/1‑150 (MQ=255)
                                                                                                                                         cGGGCGTTCCGTCAGTAACCAGTGGGCGGTAGCCTGAATTAATCCCGCCATATTTACTGCGTGGCTAAGGGTTAACGGGGTATCCATCACCCGAACCTCCACCGTGCCAAAATGAGGACTGGGGCGAATATCCCAGTGCAGATCTTTAAt              >  2:457133/1‑150 (MQ=255)
                                                                                                                                          gggCGTTCCGTCAGTAACCAGTGGGCGGTAGCCTGAATTAATCCCGCCATATTTACTGCGTGGCTAAGGGTTAACGGGGTATCCATCACCCGAACCTCCACCGTGCCAAAATGAGGACTGGGGCGAATATCCCAGTGCAGATCTTTAATg             >  2:2321375/1‑150 (MQ=255)
                                                                                                                                          gggCGTTCCGTCAGTAACCAGTGGGCGGTAGCCTGAATTAATCCCGCCATATTTACTGCGTGGCTAAGGGTTAACGGGGTATCCATCACCCGAACCTCCACCGTGCCAAAATGAGGACTGGGGCGAATATCCCAGTGCAGATCTGTAATg             >  2:3587633/1‑150 (MQ=255)
                                                                                                                                           ggCGTTCCGTAAGTAACCAGTGGGCGGTAGCCTGAATTAATCCCGCCATATTTACTGCGTGGCTAAGGGTTAACGGGGTATCCATCACCCGAACCTCCACCGTGCCAAAATGAGGACTGGGGCGAATATCCCAGTGCAGATCTTTAATGc            >  1:4856573/1‑150 (MQ=255)
                                                                                                                                              gTTCCGTCAGTAACCAGTGGGCGGTAGCCTGAATTAATCCCGCCATATTTACTGCGTGGCTAAGGGTTAACGGGGTATCCATCACCCGAACCTCCACCGTGCCAAAATGAGGACTGGGGCGAATATCCCAGTGCAGATCTTTAATGCTGt         >  1:1895942/1‑150 (MQ=255)
                                                                                                                                              gTTCCGTCAGTAACCAGTGGGCGGTAGCCTGAATTAATCCCGCCATATTTACTGCGTGGCTAAGGGTTAACGGGGTATCCATCACCCGAACCTCCACCGTGCCAAAATGAGGACTGGGGCGAATATCCCAGTGCAGATCTTTAATGCTGt         >  1:3644471/1‑150 (MQ=255)
                                                                                                                                                   gTCAGTAACCAGTGGGCGGTAGCCTGAATTAATCCCGCCATATTTACTGCGTGGCTAAGGGTTAACGGGGTATCCATCACCCGAACCTCCACCGTGCCAAAATGAGGACTGGGGCGAATATCCCAGTGCAGATCTTTAATGCTGTCGatc    <  1:3189413/150‑1 (MQ=255)
                                                                                                                                                   gTCAGTAACCAGTGGGCGGTAGCCTGAATTAATCCCGCCATATTTACTGCGTGGCTAAGGGTTAACGGGGTATCCATCACCCGAACCTCCACCGTGCCAAAATGAGGACTGGGGCGAATATCCCAGTGCAGATCTTTAATGCTGTCGatc    >  1:1354953/1‑150 (MQ=255)
                                                                                                                                                   gTCAGTAACCAGTGGGCGGTAGCCTGAATTAATCCCGCCATATTTACTGCGTGGCTAAGGGTTAACGGGGTATCCATCACCCGAACCTCCACCGTGCCAAAATGAGGACTGGGGCGAATATCCCAGTGCAGATCTTTAATGCTGTCGatc    >  1:2230968/1‑150 (MQ=255)
                                                                                                                                                   gTCAGTAACCAGTGGGCGGTAGCCTGAATTAATCACGCCATATTTACTGCGTGGCTAAGGGTTAACGGGGTATCCATCACCCGAACCTCCACCGTGCCAAAATGAGGACTGGGGCGAATATCCCAGTGCAGATCTTTAATGCTGTCGatc    >  1:3922693/1‑150 (MQ=255)
                                                                                                                                                     cAGTAACCAGTGGGCGGTAGCCTGCATTAATCCCGCCATATTGCCTGCGTGGCTAAGGGTTAACGGGGTATCCATCACCCGAACCTCCACCGTGCCAACATGAGGACTGGGGCGAATATCCCAGTGCAGATCTTTAATGCTGTCGatcat  <  2:3101743/150‑1 (MQ=255)
                                                                                                                                                     cAGTAACCAGTGGGCGGTAGCCTGAATTAATCCCGCCATATTTACTGCGTGGCTAAGGGTTAACGGGGTATCCATCACCCGAACCTCCACCGTGCCAAAATGAGGACTGGGGCGAATATCCCAGTGCAGATCTTTAATGCTGTCGatcat  <  1:250024/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
AGCAATCGCAAGGTATCTTCCGTTAGCGGTCGACGATCTCCAGTGTGCGGATCGGTGATGACGCCTTCAAGCCCATAGCGACAGGCCTGGAAACGGTTGAATTTATACAGCAGGTAATCTTTTTCCTGATGTTTAAACGGGCGTTCCGTCAGTAACCAGTGGGCGGTAGCCTGAATTAATCCCGCCATATTTACTGCGTGGCTAAGGGTTAACGGGGTATCCATCACCCGAACCTCCACCGTGCCAAAATGAGGACTGGGGCGAATATCCCAGTGCAGATCTTTAATGCTGTCGATCAT  >  CP009273/601896‑602194

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑