Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 623,935 C→A 11.3% I262I (ATC→ATA)  entB → isochorismatase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273623,9350CA11.3% 1164.2 / 83.4 333I262I (ATC→ATA) entBisochorismatase
Reads supporting (aligned to +/- strand):  ref base C (128/167);  new base A (16/22);  total (144/189)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 345 total aligned reads displayed.

GCCAGCAAAGCGGCGCTGCGTGAGGTGATCCTGCCGTTGCTGGACGAGTCCGATGAACCGTTCGATGACGACAACCTGATCGACTACGGTCTGGATTCGGTGCGCATGATGGCGCTGGCGGCGCGCTGGCGCAAAGTGCATGGTGATATCGACTTTGTCATGCTGGCGAAAAACCCGACCATCGACGCCTGGTGGAAGCTACTCTCCCGCGAGGTGAAATAATGGATTTCAGCGGTAAAAATGTCTGGGTAACCGGCGCAGGTAAAGGTATCGGCTACGCCACGGCGCTGGCGTTTGTT  >  CP009273/623786‑624084
                                                                                                                                                     |                                                                                                                                                     
gCCAGCAAAGCGGCGCTGCGTGAGGTGATCCTGCCGTTGCTGGACGAGTCCGATGAACCGTTCGATGACGACAACCTGATCGACTACGGTCTGGATTCGGTGCGCATGATGGCGCTGGCGGCGCGCTGGCGCAAAGTGCATGGTGATATc                                                                                                                                                       <  1:1443846/150‑1 (MQ=255)
gCCAGCAAAGCGGCGCTGCGTGAGGTGATCCTGCCGTTGCTGGACGAGTCCGATGAACCGTTCGATGACGACAACCTGATCGACTACGGTCTGGATTCGGTGCGCATGATGGCGCTGGCGGCGCGCTGGCGCAAAGTGCATGGTGATATc                                                                                                                                                       <  1:1861171/150‑1 (MQ=255)
 ccAGCAAAGCGGCGCTGCGTGAGGTGATCCTGCCGTTGCTGGACGAGTCCGATGAACCGTTCGATGACGACAACCTGATCGACTACGGTCTGGATTCGGTGCGCATGATGGCGCTGGCGGCGCGCTGGCGCACAGTGCATGGTGATATCg                                                                                                                                                      >  2:1464140/1‑150 (MQ=255)
  cAGCAAAGCGGCGCTGCGTGAGGTGATCCTGCCGTTGCTGGACGAGTCCGATGAACCGTTCGATGACGACAACCTGATCGACTACGGTCTGGATTCGGTGCGCATGATGGCGCTGGCGGCGCGCTGGCGCAAAGTGCATGGTGATATCGa                                                                                                                                                     <  1:2779907/150‑1 (MQ=255)
  cAGCAAAGCGGCGCTGCGTGAGGTGATCCTGCCGTTGCTGGACGAGTCCGATGAACCGTTCGATGACGACAACCTGATCGACTACGGTCTGGATTCGGTGCGCATGATGGCGCTGGCGGCGCGCTGGCGCAAAGTGCATGGTGATATCGa                                                                                                                                                     <  2:1103905/150‑1 (MQ=255)
    gCAAAGCGGCGCTGCGTGAGGTGATCCTGCCGTTGCTGGACGAGTCCGATGAACCGTTCGATGACGACAACCTGATCGACTACGGTCTGGATTCGGTGCGCATGATGGCGCTGGCGGCGCGCTGGCGCAAAGTGCATGGTGATATCGACt                                                                                                                                                   <  1:144044/150‑1 (MQ=255)
    gCAAAGCGGCGCTGCGTGAGGTGATCCTGCCGTTGCTGGACGAGTCCGATGAACCGTTCGATGACGACAACCTGATCGACAACGGTCTGGATTCGGTGCGCATGATGGCGCTGGCGGCGCGCTGGCGCAAAGTGCATGGTGATATCGACt                                                                                                                                                   >  1:2354217/1‑150 (MQ=255)
     cAAAGCGGCGCTGCGTGAGGTGATCCTGCCGTTGCTGGACGAGTCCGATGAACCGTTCGATGACGACAACCTGATCGACTACGGTCTGGATTCGGTGCGCATGATGGCGCTGGCGGCGCGCTGGCGCAAAGTGCATGGTGATATCGACtt                                                                                                                                                  <  2:3162146/150‑1 (MQ=255)
      aaaGCGGCGCTGCGTGAGGTGATCCTGCCGTTGCTGGACGAGTCCGATGAACCGTTCGATGACGACAACCTGATCGACTACGGTCTGGATTCGGTGCGCATGATGGCGCTGGCGGCGCGCTGGCGCAAAGTGCATGGTGATATCGACttt                                                                                                                                                 >  1:1484411/1‑150 (MQ=255)
      aaaGCGGCGCTGCGTGAGGTGATCCTGCCGTTGCTGGACGAGTCCGATGAACCGTTCGATGACGACAACCTGATCGACTACGGTCTGGATTCGGTGCGCATGATGGCGCTGGCGGCGCGCTGGCGCAAAGTGCATGGTGATATCGACttt                                                                                                                                                 <  2:4176676/150‑1 (MQ=255)
      aaaGCGGCGCTGCGTGAGGTGATCCAGCCGTTGCTGGACGAGTCCGATGAACCATTCGATGAAGACAACATGATCGACTCCTGTCTGGATTCGGTGCGCATGATGGAGCTGGCGGCGCGCTGGCGCAAAGTGCATGGTGATATCGACttt                                                                                                                                                 >  2:1382873/1‑150 (MQ=255)
       aaGCGGCGCTGCGTGAGGTGATCCTGCCGTTGCTGGACGAGTCCGATGAACCGTTCGATGACGACAACCTGATCGACTACGGTCTGGATTCGGTGCGCATGATGGCGCTGGCGGCGCGCTGGCGCAAAGTGCATGGTGATATAGACTTTg                                                                                                                                                <  1:593210/150‑1 (MQ=255)
        aGCGGCGCTGCGTGAGGTGATCCTGCCGTTGCTGGACGAGTCCGATGAACCGTTCGATGACGACAACCTGATCGACTACGGTCTGGATTCGGTGCGCATGATGGCGCTGGCGGCGCGCTGGCGCAAAGTGCATGGTGATATCGACTTTGt                                                                                                                                               <  2:386998/150‑1 (MQ=255)
         gcggcgCTGCGTGAGGTGATCCTGCCGTTGCTGGACGAGTCCGATGAACCGTTCGATGACGACAACCTGATCGACTACGGTCTGGATTCGGTGCGCATGATGGCGCTGGCGGCGCGCTGGCGCAAAGTGCATGGTGATATAGACTTTGTc                                                                                                                                              <  2:2301242/150‑1 (MQ=255)
          cggcgCTGCGTGAGGTGATCCTGCCGTTGCTGGACGAGTCCGATGAACCGTTCGATGACGACAACCTGATCGACTACGGTCTGGATTCGGTGCGCATGAAGGCGCTGGCGGCGCGCTGGCGCAAAGTGCATGGTGATATCGACTTTGTCa                                                                                                                                             >  2:1504404/1‑150 (MQ=255)
           ggcgCTGCGTGAGGTGATCCTGCCGTTGCTGGACGAGTCCGATGAACCGTTCGATGACGACAACCTGATCGACTACGGTCTGGATTCGGTGCGCATGATGGCGCTGGCGGCGCGCTGGCGCAAAGTGCATGGTGATATCGACTTTGTCAt                                                                                                                                            >  1:3325266/1‑150 (MQ=255)
           ggcgCTGCGTGAGGTGATCCTGCCGTTGCTGGACGAGTCCGATGAACCGTTCGATGACGACAACCTGATCGACTACGGTCTGGATTCGGTGCGCATGATGGCGCTGGCGGCGCGCTGGCGCAAAGTGCATGGTGATATCGACTTTGTCAt                                                                                                                                            >  1:5012094/1‑150 (MQ=255)
              gcTGCGTGAGGTGATCCTGCCGTTGCTGGACGAGTCCGATGAACCGTTCGATGACGACAACCTGATCGACTACGGTCTGGATTCGGTGCGCATGATGGCGCTGGCGGCGCGCTGGCGCAAAGTGCATGGTGATATCGACTTTGTCATGCt                                                                                                                                         <  1:4934618/150‑1 (MQ=255)
              gcTGCGTGAGGTGATCCTGCCGTTGCTGGACGAGTCCGATGAACCGTTCGATGACGACAACCTGATCGACTACGGTCTGGATTCGGTGCGCATGATGGCGCTGGCGGCGCGCTGGCGCAAAGTGCATGGTGATATCGACTTTGTCATGCt                                                                                                                                         <  1:4210256/150‑1 (MQ=255)
               cTGCGTGAGGTGATCCTGCCGTTGCTGGACGAGTCCGATGAACCGTTCGATGACGACAACCTGATCGACTACGGTCTGGATTCGGTGCGCATGATGGCGCTGGCGGCGCGCTGGCGCAAAGTGCATGGTGATATAGACTTTGTCATGCTg                                                                                                                                        <  1:3461283/150‑1 (MQ=255)
                 gCGTGAGGTGATCCTGCCGTTGCTGGACGAGTCCGATGAACCGTTCGATGACGACAACCTGATCGACTACGGTCTGGATTCGGTGCGCATGATGGCGCTGGCGGCGCGCTGGCGCAAAGTGCATGGTGATATCGACTTTGTCATGCTGGc                                                                                                                                      <  1:3775125/150‑1 (MQ=255)
                  cGTGAGGTGATCCTGCCGTTGCTGGACGAGTCCGATGAACCGTTCGATGACGACAACCTGATCGACTACGGTCTGGATTCGGTGCGCATGATGGCGCTGGCGGCGCGCTGGCGCAAAGTGCATGGTGATATCGACTTTGTCATGCTGGCg                                                                                                                                     <  1:3033849/150‑1 (MQ=255)
                    tGAGGTGATCCTGCCGTTGCTGGACGAGTCCGATGAACCGTTCGATGACGACAACCTGATCGACTACGGTCTGGATTCGGTGCGCATGATGGCGCTGGCGGCGCGCTGGCGCAAAGTGCATGGTGATATCGACTTTGTCATGCTGGCGaa                                                                                                                                   <  2:1940175/150‑1 (MQ=255)
                        gTGATCCTGCCGTTGCTGGACGAGTCCGATGAACCGTTCGATGACGACAACCTGATCGACTACGGTCTGGATTCGGTGCGCATGATGGCGCTGGCGGCGCGCTGGCGCAAAGTGCATGGTGATATCGACTTTGTCATGCTGGCGAAAAAc                                                                                                                               <  2:4539836/150‑1 (MQ=255)
                           aTCCTGCCGTTGCTGGACGAGTCCGATGAACCGTTCGATGACGACAACCTGATCGACTACGGTCTGGATTCGGTGCGCATGATGGCGCTGGCGGCGCGCTGGCGCAAAGTGCATGGTGATATCGACTTTGTCATGCTGGCGAAAAACCCg                                                                                                                            <  1:3984926/150‑1 (MQ=255)
                            tCCTGCCGTTGCTGGACGAGTCCGATGAACCGTTCGATGACGACAACCTGCTCGACTACGGTCTGGATTCGGTGCGCATGATGGCGCTGGCGGCGCGCTGGCGCAAAGTGCATGGTGATATCGACTTTGTCATGCTGGCGAAAAACCCGa                                                                                                                           <  1:4604355/150‑1 (MQ=255)
                             ccTGCCGTTGCTGGACGAGTCCGATGAACCGTTCGATGACGACAACCTGATCGACTACGGTCTGGATTCGGTGCGCATGATGGCGCTGGCGGCGCGCTGGCGCAAAGTGCATGGTGATATCGACTTTGTCATGCTGGCGAAAAACCCGAc                                                                                                                          <  1:819482/150‑1 (MQ=255)
                              cTGCCGTTGCTGGACGAGTCCGATGAACCGTTCGATGACGACAACCTGATCGACTACGGTCTGGATTCGGTGCGCATGATGGCGCTGGCGGCGCGCTGGCGCAAAGTGCATGGTGATATCGACTTTGTCATGCTGGCGAAAAACCCGAcc                                                                                                                         <  1:1192556/150‑1 (MQ=255)
                                 ccGTTGCTGGACGAGTCCGATGAACCGTTCGATGACGACAACCTGATCGACTACGGTCTGGATTCGGTGCGCATGATGGCGCTGGCGGCGCGCTGGCGCAAAGTGCATGGTGATATCGACTTTGTCATGCTGGCGAAAAACCCGACCATc                                                                                                                      <  2:4822271/150‑1 (MQ=255)
                                  cGTTGCTGGACGAGTCCGATGAACCGTTCGATGACGACAACCTGATCGACTACGGTCTGGATTCGGTGCGCATGATGGCGCTGGCGGCGCGCTGGCGCAAAGTGCATGGTGATATCGACTTTGTCATGCTGGCGAAAAACCCGACCATCg                                                                                                                     >  2:4498223/1‑150 (MQ=255)
                                   gTTGCTGGACGAGTCCGATGAACCGTTCGATGACGACAACCTGATCGACTACGGTCTGGATTCGGTGCGCATGATGGCGCTGGCGGCGCGCTGGCGCAAAGTGCATGGTGATATCGACTTTGTCATGCTGGCGAAAAACCCGACCATCGa                                                                                                                    <  2:196713/150‑1 (MQ=255)
                                   gTTGCTGGACGAGTCCGATGAACCGTTCGATGACGACAACCTGATCGACTACGGTCTGGATTCGGTGCGCATGATGGCGCTGGCGGCGCGCTGGCGCAAAGTGCATGGTGATATCGACTTTGTCATGCTGGCGAAAAACCCGACCATCGa                                                                                                                    <  2:3420078/150‑1 (MQ=255)
                                     tgatGGACGAGTCCGATGAACCGTTCGATGACGACAACCTGATCGACTACGGTCTGGATTCGGTGCGCATGATGGCGCTGGCGGCGCGCTGGCGCAAAGTGCATGGTGATATCGACTTTGTCATGCTGGCGAAAAACCCGACCATCGACg                                                                                                                  >  2:5081853/4‑150 (MQ=255)
                                       cTGGACGAGTCCGATGAACCGTTCGATGACGACAACCTGATCGACTACGGTCTGGATTCGGTGCGCATGATGGCGCTGGCGGCGCGCTGGCGCAAAGTGCATGGTGATATCGACTTTGTCATGCTGGCGAAAAACCCGACCATCGACGcc                                                                                                                >  1:101526/1‑150 (MQ=255)
                                        tGGACGAGTCCGATGAACCGTTCGATGACGACAACCTGATCGACTACGGTCTGGATTCGGTGCGCATGATGGCGCTGGCGGCGCGCTGGCGCAAAGTGCATGGTGATATAGACTTTGTCATGCTGGCGAAAAACCCGACCATCGACGCCt                                                                                                               <  1:2053883/150‑1 (MQ=255)
                                            cGAGTCCGATGAACCGTTCGATGACGACAACCTGATCGACTACGGTCTGGATTCGGTGCGCATGATGGCGCTGGCGGCGCGCTGGCGCAAAGTGCATGGTGATATAGACTTTGTCATGCTGGCGAAAAACCCGACCATCGACGCCtggtg                                                                                                           <  2:1786756/150‑1 (MQ=255)
                                               gTCCGATGAACCGTTCGATGACGACAACCTGATCGACTACGGTCTGGATTCGGTGCGCATGATGGCGCTGGCGGCGCGCTGGCGCAAAGTGCATGGTGATATCGACTTTGTCATGCTGGCGAAAAACCCGACCATCGACGCCTGGTGGaa                                                                                                        <  1:3728380/150‑1 (MQ=255)
                                                tCCGATGAACCGTTCGATGACGACAACCTGATCGACTACGGTCTGGATTCGGTGCGCATGATGGCGCTGGCGGCGCGCTGGCGCAAAGTGCATGGTGATATCGACTTTGTCATGCTGGCGAAAAACCCGACCATCGACGCCTGGTGGAAg                                                                                                       <  2:83857/150‑1 (MQ=255)
                                                  cGATGAACCGTTCGATGACGACAACCTGATCGACTACGGTCTGGATTCGGTGCGCATGATGGCGCTGGCGGCGCGCTGGCGCAAAGTGCATGGTGATATCGACTTTGTCATGCTGGCGAAAAACCCGACCATCGACGCCTGGTGGAAGCt                                                                                                     <  2:1603403/150‑1 (MQ=255)
                                                  cGATGAACCGTTCGATGACGACAACCTGATCGACTACGGTCTGGATTCGGTGCGCATGATGGCGCTGGCGGCGCGCTGGCGCAAAGTGCATGGTGATATCGACTTTGTCATGCTGGCGAAAAACCCGACCATCGACGCCTGGTGGAAGCt                                                                                                     <  1:2610175/150‑1 (MQ=255)
                                                   gATGAACCGTTCGATGACGACAACCTGATCGACTACGGTCTGGATTCGGTGCGCATGATGGCGCTGGCGGCGCGCTGGCGCAAAGTGCATGGTGATATCGACTTTGTCATGCTGGCGAAAAACCCGACCATCGACGCCTGGTGGAAGCTa                                                                                                    >  2:2786176/1‑150 (MQ=255)
                                                   gATGAACCGTTCGATGACGACAACCTGATCGACTACGGTCTGGATTCGGTGCGCATGATGGCGCTGGCGGCGCGCTGGCGCAAAGTGCATGGTGATATCGACTTTGTCATGCTGGCGAAAAACCCGACCATCGACGCCTGGTGGAAGCTa                                                                                                    <  2:213083/150‑1 (MQ=255)
                                                    aTGAACCGTTCGATGACGACAACCTGATCGACTACGGTCTGGATTCGGTGCGCATGATGGCGCTGGCGGCGCGCTGGCGCAAAGTGCATGGTGATATAGACTTTGTCATGCTGGCGAAAAACCCGACCATCGACGCCTGGTGGAAGCTAc                                                                                                   >  2:4040927/1‑150 (MQ=255)
                                                          cGTTCGATGACGACAACCTGATCGACTACGGTCTGGATTCGGTGCGCATGATGGCGCTGGCGGCGCGCTGGCGCAAAGTGCATGGTGATATAGACTTTGTCATGCTGGCGAAAAACCCGACCATCGACGCCTGGTGGAAGCTACTCTccc                                                                                             <  1:471045/150‑1 (MQ=255)
                                                          cGTTCGATGACGACAACCTGATAGACTACGGTCTGGATTCTGTGCGCATGATGGCGATGGCGGCGCGCTGGCGCAAATTGAATGTAGATATAGACTTTGTCATGATGGCGAAAAACACGACCATCGACGCCTGGTGGAAGCTACTCTccc                                                                                             >  1:4809589/1‑150 (MQ=255)
                                                           gTTCGATGACGACAACCTGATCGACTACGGTCTGGATTCGGTGCGCATGATGGCGCTGGCGGCGCGCTGGCGCAAAGTGCATGGTGATATCGACTTTGTCATGCTGGCGAAAAACCCGACCATCGACGCCTGGTGGAAGCTACTCTCCcg                                                                                            >  2:608452/1‑150 (MQ=255)
                                                            ttCGATGACGACAACCTGATCGACTACGGTCTGGATTCGGTGCGCATGATGGCGCTGGCGGCGCGCTGGCGCAAAGTGCATGGTGATATCGACTTTGTCATGCTGGCGAAAAACCCGACCATCGACGCCTGGTGGAAGCTACTCTCCcgc                                                                                           >  1:221561/1‑150 (MQ=255)
                                                                aTGACGACAACCTGATCGACTACGGTCTGGATTCGGTGCGCATGATGGCGCTGGCGGCGCGCTGGCGCAAAGTGCATGGTGATATCGACTTTGTCATGCTGGCGAAAAACCCGACCATCGACGCCTGGTGGAAGCTACTCTCCCGCGAgg                                                                                       <  1:542723/150‑1 (MQ=255)
                                                                 tGACGACAACCTGATCGACTACGGTCTGGATTCGGTGCGCATGATGGCGCTGGCGGCGCGCTGGCGCAAAGTGCATGGTGATATCGACTTTGTCATGCTGGCGAAAAACCCGACCATCGACGCCTGGTGGAAGCTACTCTCCCGCGAGGt                                                                                      >  2:3114411/1‑150 (MQ=255)
                                                                   acgacAACCTGATCGACTACGGTCTGGATTCGGTGCGCATGATGGCGCTGGCGGCGCGCTGGCGCAAAGTGCATGGTGATATCGACTTTGTCATGCTGGCGAAAAACCCGACCATCGACGCCTGGTGGAAGCTACTCTCCCGCGAGGTGa                                                                                    <  1:885966/150‑1 (MQ=255)
                                                                    cgacAACCTGATCGACTACGGTCTGGATTCGGTGCGCATGATGGCGCTGGCGGCGCGCTGGCGCAAAGTGCATGGTGATATCGACTTTGTCATGCTGGCGAAAAACCCGACCATCGACGCCTGGTGGAAGCTACTCTCCCGCGAGGTGaa                                                                                   <  1:2431958/150‑1 (MQ=255)
                                                                     gacAACCTGATCGACTACGGTCTGGATTCGGTGCGCATGATGGCGCTGGCGGCGCGCTGGCGCAAAGTGCATGGTGATATCGACTTTGTCATGCTGGCGAAAAACCCGACCATCGACGCCTGGTGGAAGCTACTCTCCCGCGAGGTGaaa                                                                                  <  2:3661500/150‑1 (MQ=255)
                                                                          ccTGATCGACTACGGTCTGGATTCGGTGCGCATGATGGCGCTGGCGGCGCGCTGGCGCAAAGTGCATGGTGATATCGACTTTGTCATGCTGGCGAAAAACCCGACCATCGACGCCTGGTGGAAGCTACTCTCCCGCGAGGTGAAATAATg                                                                             <  1:3421721/150‑1 (MQ=255)
                                                                               tCGACTACGGTCTGGATTCGGTGCGCATGATGGCGCTGGCGGCGCGCTGGCGCAAAGTGCATGGTGATATCGACTTTGTCATGCTGGCGAAAAACCCGACCATCGACGCCTGGTGGAAGCTACTCTCCCGCGAGGTGAAATAATGGAttt                                                                        >  1:4653083/1‑150 (MQ=255)
                                                                                cGACTACGGTCTGGAGTCGGTGCGCATGATGGCGCTGGCGGCGCGCTGGCGCAAAGTGCATGGTGATATCGACTTTGTCATGCTGGCGAAAAACCCGACCATCGACGCCTGGTGGAAGCTACTCTCCCGCGAGGTGAAATAATGGATTTc                                                                       <  1:3114218/150‑1 (MQ=255)
                                                                                 gACTACGGTCTGGATTCGGTGCGCATGATGGCGCTGGCGGCGCGCTGGCGCAAAGTGCATGGTGATATCGACTTTGTCATGCTGGCGAAAAACCCGACCATCGACGCCTGGTGGAAGCTACTCTCCCGCGAGGTGAAATAATGGATTTCa                                                                      <  2:4439570/150‑1 (MQ=255)
                                                                                 gACTACGGTCTGGATTCGGTGCGCATGATGGCGCTGGCGGCGCGCTGGCGCAAAGTGCATGGTGATATCGACTTTGTCATGCTGGCGAAAAACCCGACCATCGACGCCTGGTGGAAGCTACTCTCCCGCGAGGTGAAATAATGGATTTCa                                                                      >  1:1033695/1‑150 (MQ=255)
                                                                                  aCTACGGTCTGGATTCGGTGCGCATGATGGCGCTGGCGGCGCGCTGGCGCAAAGTGCATGGTGATATCGACTTTGTCATGCTGGCGAAAAACCCGACCATCGACGCCTGGTGGAAGCTACTCTCCCGCGAGGTGAAATAATGGATTTCAg                                                                     <  2:3482672/150‑1 (MQ=255)
                                                                                      cGGTCTGGATTCGGTGCGCATGATGGCGCTGGCGGCGCGCTGGCGCAAAGTGCATGGTGATATCGACTTTGTCATGCTGGCGAAAAACCCGACCATCGACGCCTGGTGGAAGCTACTCTCCCGCGAGGTGAAATAATGGATTTCAGCGGt                                                                 >  1:748137/1‑150 (MQ=255)
                                                                                       ggTCTGGATTCGGTGCGCATGATGGCGCTGGCGGCGCGCTGGCGCAAAGTGCATGGTGATATCGACTTTGTCATGCTGGCGAAAAACCCGACCATCGACGCCTGGTGGAAGCTACTCTCCCGCGAGGTGAAATAATGGATTTCAGCGGTa                                                                <  1:1326349/150‑1 (MQ=255)
                                                                                         tCTGGATTCGGTGCGCATGATGGCGCTGGCGGCGCGCTGGCGCAAAGTGCATGGTGATATCGACTTTGTCATGCTGGCGAAAAACCCGACCATCGACGCCTGGTGGAAGCTACTCTCCCGCGAGGTGAAATAATGGATTTCAGCGGTaaa                                                              <  2:4950981/150‑1 (MQ=255)
                                                                                         tCTGGATTCGGTGCGCATGATGGCGCTGGCGGCGCGCTGGCGCAAAGTGCATGGTGATATCGACTTTGTCATGCTGGCGAAAAACCCGACCATCGACGCCTGGTGGAAGCTACTCTCCCGCGAGGTGAAATAATGGATTTCAGCGGTaaa                                                              <  2:1836070/150‑1 (MQ=255)
                                                                                          cTGGATTCGGTGCGCATGATGGCGCTGGCGGCGCGCTGGCGCAAAGTGCATGGTGATATCGACTTTGTCATGCTGGCGAAAAACCCGACCATCGACGCCTGGTGGAAGCTACTCTCCCGCGAGGTGAAATAATGGATTTCAGCGGTaaaa                                                             <  2:53095/150‑1 (MQ=255)
                                                                                           tGGATTCGGTGCGCATGATGGCGCTGGCGGCGCGCTGGCGCAAAGTGCATGGTGATATCGACTTTGTCATGCTGGCGAAAAACCCGACCATCGACGCCTGGTGGACGCTACTCTCCCGCGAGGTGAAATAATGGATTTCAGCGGTaaaaa                                                            >  2:2072699/1‑150 (MQ=255)
                                                                                            ggATTCGGTGCGCATGATGGCGCTGGCGGCGCGCTGGCGCAAAGTGCATGGTGATATCGACTTTGTCATGCTGGCGAAAAACCCGACCATCGACGCCTGGTGGAAGCTACTCTCCCGCGAGGTGAAATAATGGATTTCAGCGGTAAAAAt                                                           <  2:4676752/150‑1 (MQ=255)
                                                                                             gATTCGGTGCGCATGATGGCGCTGGCGGCGCGCTGGCGCAAAGTGCATGGTGATATCGACTTTGTCATGCTGGCGAAAAACCCGACCATCGACGCCTGGTGGAAGCTACTCTCCCGCGAGGTGAAATAATGGATTTCAGCGGTAAAAATg                                                          >  2:1168802/1‑150 (MQ=255)
                                                                                             gATTCGGTGCGCATGATGGCGCTGGCGGCGCGCTGGCGCAAAGTGCATGGTGATATCGACTTTGTCATGCTGGCGAAAAACCCGACCATCGACGCCTGGTGGAAGCTACTCTCCCGCGAGGTGAAATAATGGATTTCAGCGGTAAAAATg                                                          >  2:3930922/1‑150 (MQ=255)
                                                                                             gATTCGGTGCGCATGATGGCGCTGGCGGCGCGCTGGCGCAAAGTGCATGGTGATATCGACTTTGTCATGCTGGCGAAAAACCCGACCATCGACGCCTGGTGGAAGCTACTCTCCCGCGAGGTGAAATAATGGATTTCAGCGGTAAAAATg                                                          >  2:3124465/1‑150 (MQ=255)
                                                                                              aTTCGGTGCGCATGATGGCGCTGGCGGCGCGCTGGCGCAAAGTGCATGGTGATATCGACTTTGTCATGCTGGCGAAAAACCCGACCATCGACGCCTGGTGGAAGCTACTCTCCCGCGAGGTGAAATAATGGATTTCAGCGGTAAAAATGt                                                         >  2:433982/1‑150 (MQ=255)
                                                                                               ttCGGTGCGCATGATGGCGCTGGCGGCGCGCTGGCGCAAAGTGCATGGTGATATCGACTTTGTCATGCTGGCGAAAAACCCGACCATCGACGCCTGGTGGAAGCTACTCTCCCGCGAGGTGAAATAATGGATTTCAGCGGTAAAAATGTc                                                        <  1:2669928/150‑1 (MQ=255)
                                                                                                    tGCGCATGATGGCGCTGGCGGCGCGCTGGCGCAAAGTGCATGGTGATATCGACTTTGTCATGCTGGCGAAAAACCCGACCATCGACGCCTGGTGGAAGCTACTCTCCCGCGAGGTGAAATAATGGATTTCAGCGGTAAAAATGTCTGGGt                                                   <  1:4184844/150‑1 (MQ=255)
                                                                                                     gcgcATGATGGCGCTGGCGGCGCGCTGGCGCAAAGTGCATGGTGATATCGACTTTGTCATGCTGGCGAAAAACCCGACCATCGACGCCTGGTGGAAGCTACTCTCCCGCGAGGTGAAATAATGGATTTCAGCGGTAAAAATGTCTGGGTa                                                  <  1:2536225/150‑1 (MQ=255)
                                                                                                     gcgcATGATGGCGCTGGCGGCGCGCTGGCGCAAAGTGCATGGTGATATCGACTTTGTCATGCTGGCGAAAAACCCGACCATCGACGCCTGGTGGAAGCTACTCTCCCGCGAGGTGAAATAATGGATTTCAGCGGTAAAAATGTCTGGGTa                                                  <  2:3305157/150‑1 (MQ=255)
                                                                                                        cATGATGGCGCTGGCGGCGCGCTGGCGCAAAGTGCATGGTGATATCGACTTTGTCATGCTGGCGAAAAACCCGACCATCGACGCCTGGTGGAAGCTACTCTCCCGCGAGGTGAAATAATGGATTTCAGCGGTAAAAATGTCTGGGTAAcc                                               >  1:1386040/1‑150 (MQ=255)
                                                                                                            atgGCGCTGGCGGCGCGCTGGCGCAAAGTGCATGGTGATATCGACTTTGTCATGCTGGCGAAAAACCCGACCATCGACGCCTGGTGGAAGCTACTCTCCCGCGAGGTGAAATAATGGATTTCAGCGGTAAAAATGTCTGGGTAACCGgcg                                           <  2:2448794/150‑1 (MQ=255)
                                                                                                              ggCGCTGGCGGCGCGCTGGCGCAAAGTGCATGGTGATATCGACTTTGTCATGCTGGCGAAAAACCCGACCATCGACGCCTGGTGGAAGCTACTCTCCCGCGAGGTGAAATAATGGATTTCAGCGGTAAAAATGTCTGGGTAACCGGCGCa                                         >  1:4100058/1‑150 (MQ=255)
                                                                                                                cgcTGGCGGCGCGCTGGCGCAAAGTGCATGGTGATATCGACTTTGTCATGCTGGCGAAAAACCCGACCATCGACGCCTGGTGGAAGCTACTCTCCCGCGAGGTGAAATAATGGATTTCAGCGGTAAAAATGTCTGGGTAACCGGCGCAgg                                       <  1:3158673/150‑1 (MQ=255)
                                                                                                                 gcTGGCGGCGCGCTGGCGCAAAGTGCATGGTGATATCGACTTTGTCATGCTGGCGAAAAACCCGACCATCGACGCCTGGTGGAAGCTTCTCTCCCGCGAGGTGAAATAATGGATTTCAGCTGTAAAAATGTCTGGGTAACCGGCTCAGGt                                      <  2:2354217/150‑1 (MQ=255)
                                                                                                                   tGGCGGCGCGCTGGCGCAAAGTGCATGGTGATATCGACTTTGTCATGCTGGCGAAAAACCCGACCATCGACGCCTGGTGGAAGCTACTCTCCCGCGAGGTGAAATAATGGATTTCAGCGGTAAAAATGTCTGGGTAACCGGCGCAGGTaa                                    >  1:687967/1‑150 (MQ=255)
                                                                                                                   tGGCGGCGCGCTGGCGCAAAGTGCATGGTGATATCGACTTTGTCATGCTGGCGAAAAACCCGACCATCGACGCCTGGTGGAAGCTACTCTCCCGCGAGGTGAAATAATGGATTTCAGCGGTAAAAATGTCTGGGTAACCGGCGCAGGTaa                                    >  1:4461606/1‑150 (MQ=255)
                                                                                                                    ggcggcGCGCTGGCGCAAAGTGCATGGTGATATCGACTTTGTCATGCTGGCGAAAAACCCGACCATCGACGCCTGGTGGAAGCTACTCTCCCGCGAGGTGAAATAATGGATTTCAGCGGTAAAAATGTCTGGGTAACCGGCGCAGGTaaa                                   <  1:4350371/150‑1 (MQ=255)
                                                                                                                       ggcgCGCTGGCGCAAAGTGCATGGTGATATCGACTTTGTCATGCTGGCGAAAAACCCGACCATCGACGCCTGGTGGAAGCTACTCTCCCGCGAGGTGAAATAATGGATTTCAGCGGTAAATATGTCTGGGTAACCGGCGCAGGTAAAGGt                                <  2:256895/150‑1 (MQ=255)
                                                                                                                       ggcgCGCTGGCGCAAAGTGCATGGTGATATCGACTTTGTCATGCTGGCGAAAAACCCGACCATCGACGCCTGGTGGAAGCTACTCTCCCGCGAGGTGAAATAATGGATTTCAGCGGTAAAAATGTCTGGGTAACCGGCGCAGGTAAAGGt                                >  2:2155666/1‑150 (MQ=255)
                                                                                                                         cgcgcTGGCGCAAAGTGCATGGTGATATCGACTTTGTCATGCTGGCGAAAAACCCGACCATCGACGCCTGGTGGAAGCTACTCTCCCGCGAGGTGAAATAATGGATTTCAGCGGTAAAAATGTCTGGGTAACCGGCGCAGGTAAAGGTAt                              >  2:4907191/1‑150 (MQ=255)
                                                                                                                         cgcgcTGGCGCAAAGTGCATGGTGATATCGACTTTGTCATGCTGGCGAAAAACCCGACCATCGACGCCTGGTGGAAGCTACTCTCCCGCGAGGTGAAATAATGGATTTCAGCGGTAAAAATGTCTGGGTAACCGGCGCAGGTAAAGGTAt                              <  2:4065779/150‑1 (MQ=255)
                                                                                                                         cgcgcTGGCGCAAAGTGCATGGTGATATAGACTTTGTCATGCTGGCGAAAAACCCGACCATCGACGCCTGGTGGAAGCTACTCTCCCGCGAGGTGAAATAATGGATTTCAGCGGTAAAAATGTCTGGGTAACCGGCGCAGGTAAAGGTAt                              >  2:2925984/1‑150 (MQ=255)
                                                                                                                           cgcTGGCGCAAAGTGCATGGTGATATCGACTTTGTCATGCTGGCGAAAAACCCGACCATCGACGCCTGGTGGAAGCTACTCTCCCGCGAGGTGAAATAATGGATTTCAGCGGTAAAAATGTCTGGGTAACCGGCGCAGGTAAAGGTATCg                            <  2:1674019/150‑1 (MQ=255)
                                                                                                                            gcTGGCGCAAAGTGCATGGTGATATCGACTTTGTCATGCTGGCGAAAAACCCGACCATCGACGCCTGGTGGAAGCTACTCTCCCGCGAGGTGAAATAATGGATTTCAGCGGTAAAAATGTCTGGGTAACCGGCGCAGGTAAAGGTATCgg                           >  2:5090989/1‑150 (MQ=255)
                                                                                                                               ggCGCAAAGTGCATGGTGATATCGACTTTGTCATGCTGGCGAAAAACCCGACCATCGACGCCTGGTGGAAGCTACTCTCCCGCGAGGTGAAATAATGGATTTCAGCGGTAAAAATGTCTGGGTAACCGGCGCAGGTAAAGGTATCGGCTa                        >  2:3852439/1‑150 (MQ=255)
                                                                                                                                 cgcAAAGTGCATGGTGATATCGACTTTGTCATGCTGGCGAAAAACCCGACCATCGACGCCTGGTGGAAGCTACTCTCCCGCGAGGTGAAATAATGGATTTCAGCGGTAAAAATGTCTGGGTAACCGGCGCAGGTAAAGGTATCGGCTACg                      <  1:2275400/150‑1 (MQ=255)
                                                                                                                                  gcAAAGTGCATGGTGATATCGACTTTGTCATGCTGGCGAAAAACCCGACCATCGACGCCTGGTGGAAGCTACTCTCCCGCGAGGTGAAATAATGGATTTCAGCGGTAAAAATGTCTGGGTAACCGGCGCAGGTAAAGGTATCGGCTACGc                     >  1:212523/1‑150 (MQ=255)
                                                                                                                                   cAAAGTGCATGGTGATATCGACTTTGTCATGCTGGCGAAAAACCCGACCATCGACGCCTGGTGGAAGCTACTCTCCCGCGAGGTGAAATAATGGATTTCAGCGGTAAAAATGTCTGGGTAACCGGCGCAGGTAAAGGTATCGGCTACGcc                    <  1:2947133/150‑1 (MQ=255)
                                                                                                                                     aaGTGCATGGTGATATCGACTTTGTCATGCTGGCGAAAAACCCGACCATCGACGCCTGGTGGAAGCTACTCTCCCGCGAGGTGAAATAATGGATTTCAGCGGTAAAAATGTCTGGGTAACCGGCTCAGGTAAAGGTATCGGCTACGCCAc                  <  1:5081853/150‑1 (MQ=255)
                                                                                                                                     aaGTGCATGGTGATATAGACTTTGTCATGCTGGCGAAAAACCCGACCATCGACGCCTGGTGGAAGCTACTCTCCCGCGAGGTGAAATAATGGATTTCAGCGGTAAAAATGTCTGGGTAACCGGCGCAGGTAAAGGTATCGGCTACGCCAc                  <  2:2876511/150‑1 (MQ=255)
                                                                                                                                         gCATGGTGATATCGACTTTGTCATGCTGGCGAAAAACCCGACCATCGACGCCTGGTGGAAGCTACTCTCCCGCGAGGTGAAATAATGGATTTCAGCGGTAAAAATGTCTGGGTAACCGGCGCAGGTAAAGGTATCGGCTACGCCACGgcg              >  1:4079527/1‑150 (MQ=255)
                                                                                                                                             ggTGATATAGACTTTGTCATGCTGGCGAAAAACCCGACCATCGACGCCTGGTGGAAGCTACTCTCCCGCGAGGTGAAATAATGGATTTCAGCGGTAAAAATGTCTGGGTAACCGGCGCAGGTAAAGGTATCGGCTACGCCACGGCGCTgg          <  2:4988635/150‑1 (MQ=255)
                                                                                                                                                  tatCGACTTTGTCATGCTGGCGAAAAACCCGACCATCGACGCCTGGTGGAAGCTACTCTCCCGCGAGGTGAAATAATGGATTTCAGCGGTAAAAATGTCTGGGTAACCGGCGCAGGTAAAGGTATCGGCTACGCCACGGCGCTGGCGttt     <  2:901818/150‑1 (MQ=255)
                                                                                                                                                   atCGACTTTGTCATGCTGGCGAAAAACCCGACCATCGACGCCTGGTGGAAGCTACTCTCCCGCGAGGTGAAATAATGGATTTCAGCGGTAAAAATGTCTGGGTAACCGGCGCAGGTAAAGGTATCGGCTACGCCACGGCGCTGGCGTttg    >  1:1597135/1‑150 (MQ=255)
                                                                                                                                                     cGACTTTGTCATGCTGGCGAAAAACCCGACCATCGACGCCTGGTGGAAGCTACTCTCCCGCGAGGTGAAATAATGGATTTCAGCGGTAAAAATGTCTGGGTAACCGGCGCAGGTAAAGGTATCGGCTACGCCACGGCGCTGGCGTttgtt  >  1:4387279/1‑150 (MQ=255)
                                                                                                                                                     cGACTTTGTCATGCTGGCGAAAAACCCGACCATCGACGCCTGGTGGAAGCTACTCTCCCGCGAGGTGAAATAATGGATTTCAGCGGTAAAAATGTCTGGGTAACCGGCGCAGGTAAAGGTATCGGCTACGCCACGGCGCTGGCGTttgtt  >  2:1776789/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
GCCAGCAAAGCGGCGCTGCGTGAGGTGATCCTGCCGTTGCTGGACGAGTCCGATGAACCGTTCGATGACGACAACCTGATCGACTACGGTCTGGATTCGGTGCGCATGATGGCGCTGGCGGCGCGCTGGCGCAAAGTGCATGGTGATATCGACTTTGTCATGCTGGCGAAAAACCCGACCATCGACGCCTGGTGGAAGCTACTCTCCCGCGAGGTGAAATAATGGATTTCAGCGGTAAAAATGTCTGGGTAACCGGCGCAGGTAAAGGTATCGGCTACGCCACGGCGCTGGCGTTTGTT  >  CP009273/623786‑624084

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑