Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 650,141 G→T 15.1% V428V (GTC→GTA)  dcuC ← anaerobic C4‑dicarboxylate transport

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273650,1410GT15.1% 1171.1 / 135.6 373V428V (GTC→GTA) dcuCanaerobic C4‑dicarboxylate transport
Reads supporting (aligned to +/- strand):  ref base G (178/138);  new base T (29/28);  total (207/166)
Fisher's exact test for biased strand distribution p-value = 4.71e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.35e-01

Only 100 of 375 total aligned reads displayed.

GTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGCTCTGTAGCAACGATAACAATCACCAAACCAACAAGCACCGGTACCGAGGTGCGTTTTACGACTTCAAACGGCGAGATCTTCGCCATCCCGGCAACCGCAACGACTACGCCAGAAACGGGCGAAAGGGTACGGCCAAGGTTTGACGCCTGCAGCATCGGGATAGTCAAATACGCCGGGTTAATGCCGGAAGAGTGCGCCAGTTTCGGGAT  >  CP009273/649995‑650290
                                                                                                                                                  |                                                                                                                                                     
gTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGGCCGCTGCTGCCGTTCCTGGCACCATCAGCTCTGTAGCAACGATAACAATCACCAAACCAACAAGCACCGGTACCGAGGTGCGTTTTACGACt                                                                                                                                                    >  1:1966260/1‑150 (MQ=255)
 tGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGCTCTGTAGCAACGATAACAATCACCAAACCAACAAGCACCGGTACCGAGGTGCGTTTTACTACtt                                                                                                                                                   >  1:4553174/1‑150 (MQ=255)
 tGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGCTCTGTAGCAACGATAACAATCACCAAACCAACAAGCACCGGTACCGAGGTGCGTTTTACGACtt                                                                                                                                                   >  1:4626923/1‑150 (MQ=255)
     aTGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGCTCTGTAGCAACGATAACAATCACCAAACCAACAAGCACCGGTACCGAGGTGCGTTTTACTACTTCaaa                                                                                                                                               >  2:2183525/1‑150 (MQ=255)
       gATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGCTCTGTAGCAACGATAACAATCACCAAACCAACAAGCACCGGTACCGAGGTGCGTTTTACTACTTCAAACg                                                                                                                                             >  2:3874142/1‑150 (MQ=255)
       gATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGCTCTGTAGCAACGATAACAATCACCAAACCAACAAGCACCGGTACCGAGGTGCGTTTTACGACTTCAAACg                                                                                                                                             <  2:1247771/150‑1 (MQ=255)
         tAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGCTCTGTAGCAACGATAACAATCACCAAACCAACAAGCACCGGTACCGAGGTGCGTTTTACTACTTCAAACGGc                                                                                                                                           >  2:395559/1‑150 (MQ=255)
          aaaGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGCTCTGTAGCAACGATAACAATCACCAAACCAACAAGCACCGGTACCGAGGTGCGTTTTACGACTTCAAACGGCg                                                                                                                                          >  2:3066150/1‑150 (MQ=255)
            aGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGCTCTGTAGCAACGATAACAATCACCAAACCAACAAGCACCGGTACCGAGGTGCGTTTTACGACTTCAAACGGCgag                                                                                                                                        <  2:1871900/150‑1 (MQ=255)
            aGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGCTCTGTAGCAACGATAACAATCACCAAACCAACAAGCACCGGTACCGAGGTGCGTTTTACGACTTCAAACGGCgag                                                                                                                                        <  2:2788234/150‑1 (MQ=255)
             gCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGCTCTGTAGCAACGATAACAATCACCAACCCAACAAGCACCGGTACCGAGGTGCGTTTTACGACTTCACACGGCgaga                                                                                                                                       >  2:3209626/1‑150 (MQ=255)
               cGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGCTCTGTAGCAACGATAACAATCACCAAACCAACAAGCACCGGTACCGAGGTGCGTTTTACGACTTCAAACGGCGAGATc                                                                                                                                     >  2:3995016/1‑150 (MQ=255)
               cGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGCTCTGTAGCAACGATAACAATCACCAAACCAACAAGCACCGGTACCGAGGTGCGTTTTACGACTTCAAACGGCGAGATc                                                                                                                                     >  1:666998/1‑150 (MQ=255)
                ggaggaAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGCTCTGTAGCAACGATAACAATCACCAAACCAACAAGCACCGGTACCGAGGTGCGTTTTACGACTTCAAACGGCGAGATCt                                                                                                                                    >  1:136156/1‑150 (MQ=255)
                 gaggaAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGCTCTGTAGCAACGATAACAATCACCAAACCAACAAGCACCGGTACCGAGGTGCGTTTTACTACTTCAAACGGCGAGATCtt                                                                                                                                   >  2:4350656/1‑150 (MQ=255)
                 gaggaAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGCTCTGTAGCAACGATAACAATCACCAAACCAACAAGCACCGGTACCGAGGTGCGTTTTACGACTTCAAACGGCGAGATCtt                                                                                                                                   >  2:227343/1‑150 (MQ=255)
                     aaaCTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGCTCTGTAGCAACGATAACAATCACCAAACCAACAAGCACCGGTACCGAGGTGCGTTTTACTACTTCAAACGGCGAGATCTTCGcc                                                                                                                               >  2:3734293/1‑150 (MQ=255)
                         ttGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGCTCTGTAGCAACGATAACAATCACCAAACCAACAAGCACCGGTACCGAGGTGCGTTTTACGACTTCAAACGGCGAGATCTTCGCCATcc                                                                                                                           <  2:4576450/150‑1 (MQ=255)
                         ttGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGCTCTGTAGCAACGATAACAATCACCAAACCAACAAGCACCGGTACCGAGGTGCGTTTTACGACTTCAAACGGCGAGATCTTCGCCATcc                                                                                                                           >  1:1053020/1‑150 (MQ=255)
                            ccTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGCTCTGTAGCAACGATAACAATCACCAAACCAACAAGCACCGGTACCGAGGTGCGTTTTACGACTTCAAACGGCGAGATCTTCGCCATCCCgg                                                                                                                        <  2:3824754/150‑1 (MQ=255)
                             cTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGCTCTGTAGCAACGATAACAATCACCAACCCACCAAGCACCGGTACCGAGGTGCGTTTTACGACTTCAAACGGCGAGATCTTCGCCATCCCGGc                                                                                                                       <  2:1309578/150‑1 (MQ=255)
                               ccGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGCTCTGTAGCAACGATAACAATCACCAAACCAACAAGCACCGGTACCGAGGTGCGTTTTACTACTTCAAACGGCGAGATCTTCGCCATCCCGGCaa                                                                                                                     <  1:2296868/150‑1 (MQ=255)
                               ccGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGCTCTGTAGCAACGATAACAATCACCAAACCAACAAGCACCGGTACCGAGGTGCGTTTTACGACTTCAAACGGCGAGATCTTCGCCATCCCGGCaa                                                                                                                     <  1:1020231/150‑1 (MQ=255)
                                cGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGCTCTGTAGCAACGATAACAATCACCAAACCAACAAGCACCGGTACCGAGGTGCGTTTTACGACTTCAAACGGCGAGATCTTCGCCATCCCGGCAAc                                                                                                                    >  2:3764292/1‑150 (MQ=255)
                                 ggCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGCTCTGTAGCAACGATAACAATCACCAAACCAACAAGCACCGGTACCGAGGTGCGTTTTACGACTTCAAACGGCGAGATCTTCGCCATCCCGGCAAcc                                                                                                                   >  2:3511628/1‑150 (MQ=255)
                                 ggCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGCTCTGTAGCAACGATAACAATCACCAAACCAACAAGCACCGGTACCGAGGTGCGTTTTACGACTTCAAACGGCGAGATCTTCGCCATCCCGGCAAcc                                                                                                                   <  1:3409242/150‑1 (MQ=255)
                                 ggCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGCTCTGTAGCAACGATAACAATCACCAAACCAACAAGCACCGGTACCGAGGTGCGTTTTACGACTTCAAACGGCGAGATCTTCGCCATCCCGGCAAcc                                                                                                                   >  2:484556/1‑150 (MQ=255)
                                          aTTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGCTCTGTAGCAACGATAACAATCACCAAACCAACAAGCACCGGTACCGAGGTGCGTTTTACGACTTCAAACGGCGAGATCTTCGCCATCCCGGCAACCGCAACGACt                                                                                                          <  2:1037983/150‑1 (MQ=255)
                                          aTTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGCTCTGTAGCAACGATAACAATCACCAAACCAACAAGCACCGGTACCGAGGTGCGTTTTACGACTTCAAACGGCGAGATCTTCGCCATCCCGGCAACCGCAACGACt                                                                                                          <  1:4381694/150‑1 (MQ=255)
                                            ttACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGCTCTGTAGCAACGATAACAATCACCAAACCAACAAGCACCGGTACCGAGGTGCGTTTTACGACTTCAAACGGCGAGATCTTCGCCATCCCGGCAACCGCAACGACTAc                                                                                                        >  2:16292/1‑150 (MQ=255)
                                                ttGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGCTCTGTAGCAACGATAACAATCACCAAACCAACAAGCACCGGTACCGAGGTGCGTTTTACGACTTCAAACGGCGAGATCTTCGCCATCCCGGCAACCGCAACGACTACGCCa                                                                                                    <  1:3897370/150‑1 (MQ=255)
                                                 tGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGCTCTGTAGCAACGATAACAATCACCAAACCAACAAGCACCGGTACCGAGGTGCGTTTTACTACTTCAAACGGCGAGATCTTCGCCATCCCGGCAACCGCAACGACTACGCCAg                                                                                                   >  2:1494854/1‑150 (MQ=255)
                                                  gCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGCTCTGTAGCAACGATAACAATCACCAAACCAACAAGCACCGGTACCGAGGTGCGTTTTACGACTTCAAACGGCGAGATCTTCGCCATCCCGGCAACCGCAACGACTACGCCAGa                                                                                                  >  1:5025897/1‑150 (MQ=255)
                                                   ccTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGCTCTGTAGCAACGATAACAATCACCAAACCAACAAGCACCGGTACCGAGGTGCGTTTTACTACTTCAAACGGCGAGATCTTCGCCATCCCGGCAACCGCAACGACTACGCCAGaa                                                                                                 >  1:3699312/1‑150 (MQ=255)
                                                      gtgACCGCTGCTGCCGTTCCTGGCACCATCAGCTCTGTAGCAACGATAACAATCACCAAACCAACAAGCACCGGTACCGAGGTGCGTTTTACTACTTCAAACGGCGAGATCTTCGCCATCCCGGCAACCGCAACGACTACGCCAGAAACg                                                                                              <  2:4787471/150‑1 (MQ=255)
                                                      gtgACCGCTGCTGCCGTTCCTGGCACCATCAGCTCTGTAGCAACGATAACAATCACCAAACCAACAAGCACCGGTACCGAGGTGCGTTTTACGACTTCAAACGGCGAGATCTTCGCCATCCCGGCAACCGCAACGACTACGCCAGAAACg                                                                                              <  2:1946527/150‑1 (MQ=255)
                                                      gtgACCGCTGCTGCCGTTCCTGGCACCATCAGCTCTGTAGCAACGATAACAATCACCAAACCAACAAGCACCGGTACCGAGGTGCGTTTTACGACTTCAAACGGCGAGATCTTCGCCATCCCGGCAACCGCAACGACTACGCCAGAAACg                                                                                              >  2:2008584/1‑150 (MQ=255)
                                                       tgACCGCTGCTGCCGTTCCTGGCACCATCAGCTCTGTAGCAACGATAACAATCACCAAACCAACAAGCACCGGTACCGAGGTGCGTTTTACGACTTCAAACGGCGAGATCTTCGCCATCCCGGCAACCGCAACGACTACGCCAGAAACgg                                                                                             <  1:225894/150‑1 (MQ=255)
                                                        gACCGCTGCTGCCGTTCCTGGCACCATCAGCTCTGTAGCAACGATAACAATCACCAAACCAACAAGCACCGGTACCGAGGTGCGTTTTACGACTTCAAACGGCGAGATCTTCGCCATCCCGGCAACCGCAACGACTACGCCAGAAACggg                                                                                            >  2:1428497/1‑150 (MQ=255)
                                                        gACCGCTGCTGCCGTTCCTGGCACCATCAGCTCTGTAGCAACGATAACAATCACCAAACCAACAAGCACCGGTACCGAGGTGCGTTTTACGACTTCAAACGGCGAGATCTTCGCCATCCCGGCAACCGCAACGACTACGCCAGAAACggg                                                                                            <  1:3363595/150‑1 (MQ=255)
                                                        gACCGCTGCTGCCGTTCCTGGCACCATCAGCTCTGTAGCAACGATAACAATCACCAAACCAACAAGCACCGGTACCGAGGTGCGTTTTACGACTTCAAACGGCGAGATCTTCGCCATCCCGGCAACCGCAACGACTACGCCAGAAACggg                                                                                            <  1:1636740/150‑1 (MQ=255)
                                                        gACCGCTGCTGCCGTTCCTGGCACCATCAGCTCTGTAGCAACGATAACAATCACCAAACCAACAAGCACCGGTACCGAGGTGCGTTTTACGACTTCAAACGGCGAGATCTTCGCCATCCCGGCAACCGCAACGACTACGCCAGAAACggg                                                                                            >  1:4065066/1‑150 (MQ=255)
                                                           cGCTGCTGCCGTTCCTGGCACCATCAGCTCTGTAGCAACGATAACAATCACCAAACCAACAAGCACCGGTACCGAGGTGCGTTTTACGACTTCAAACGGCGAGATCTTCGCCATCCCGGCAACCGCAACGACTACGCCAGAAACGGGCGa                                                                                         >  2:1182167/1‑150 (MQ=255)
                                                               gctgcCGTTCCTGGCACCATCAGCTCTGTAGCAACGATAACAATCACCAAACCAACACGCACCGGTACCGAGGTGCGTTTTACTACTTCAAACGGCGAGATCTTCGCCATCCCGGCAACCGCAACGACTACGCCAGAAACGGGCGAAAgg                                                                                     <  1:2064800/150‑1 (MQ=255)
                                                               gctgcCGTTCCTGGCACCATCAGCTCTGTAGCAACGATAACAATCACCAAACCAACAAGCACCGGTACCGAGGTGCGTTTTACGACTTCAAACGGCGAGATCTTCGCCATCCCGGCAACCGCAACGACTACGCCAGAAACGGGCGAAAgg                                                                                     >  1:2515115/1‑150 (MQ=255)
                                                                 tgcCGTTCCTGGCACCATCAGCTCTGTAGCAACGATAACAATCACCAAACCAACAAGCACCGGTACCGAGGTGCGTTTTACGACTTCAAACGGCGAGATCTTCGCCATCCCGGCAACCGCAACGACTACGCCAGAAACGGGCGAAAGGGt                                                                                   <  1:490520/150‑1 (MQ=255)
                                                                  gcCGTTCCTGGCACCATCAGCTCTGTAGCAACGATAACAATCACCAAACCAACAAGCACCGGTACCGAGGTGCGTTTTACTACTTCAAACGGCGAGATCTTCGCCATCCCGGCAACCGCAACGACTACGCCAGAAACGGGCGAAAGGGTa                                                                                  <  1:4703951/150‑1 (MQ=255)
                                                                    cGTTCCTGGCACCATCAGCTCTGTAGCAACGATAACAATCACCAAACCAACAAGCACCGGTACCGAGGTGCGTTTTACGACTTCAAACGGCGAGATCTTCGCCATCCCGGCAACCGCAACGACTACGCCAGAAACGGGCGAAAGGGTACg                                                                                <  1:2390521/150‑1 (MQ=255)
                                                                    cGTTCCTGGCACCATCAGCTCTGTAGCAACGATAACAATCACCAAACCAACAAGCACCGGTACCGAGGTGCGTTTTACGACTTCAAACGGCGAGATCTTCGCCATCCCGGCAACCGCAACGACTACGCCAGAAACGGGCGAAAGGGTACg                                                                                <  1:915288/150‑1 (MQ=255)
                                                                       tCCTGGCACCATCAGCTCTGTAGCAACGATAACAATCACCAAACCAACAAGCACCGGTACCGAGGTGCGTTTTACTACTTCAAACGGCGAGATCTTCGCCATCCCGGCAACCGCAACGACTACGCCAGAAACGGGCGAAAGGGTACGGcc                                                                             >  2:4435973/1‑150 (MQ=255)
                                                                         cTGGCACCATCAGCTCTGTAGCAACGATAACAATCACCAAACCAACAAGCACCGGTACCGAGGTGCGTTTTACTACTTCAAACGGCGAGATCTTCGCCATCCCGGCAACCGCAACGACTACGCCAGAAACGGGCGAAAGGGTACGGCCaa                                                                           <  2:2664246/150‑1 (MQ=255)
                                                                           ggCACCATCAGCTCTGTAGCAACGATAACAATCACCAAACCAACAAGCACCGGTACCGAGGTGCGTTTTACGACTTCAAACGGCGAGATCTTCGCCATCCCGGCAACCGCAACGACTACGCCAGAAACGGGCGAAAGGGTACGGCCAAgg                                                                         >  1:3119108/1‑150 (MQ=255)
                                                                                cATCAGCTCTGTAGCAACGATAACAATCACCAAACCAACAAGCACCGGTACCGAGGTGCGTTTTACGACTTCAAACGGCGAGATCTTCGCCATCCCGGCAACCGCAACGACTACGCCAGAAACGGGCGAAAGGGTACGGCCAAGGTTTGa                                                                    <  1:317091/150‑1 (MQ=255)
                                                                                cATCAGCTCTGTAGCAACGATAACAATCACCAAACCAACAAGCACCGGTACCGAGGTGCGTTTTACGACTTCAAACGGCGAGATCTTCGCCATCCCGGCAACCGCAACGACTACGCCAGAAACGGGCGAAAGGGTACGGCCAAGGTTTGa                                                                    >  1:3279032/1‑150 (MQ=255)
                                                                                   cAGCTCTGTAGCAACGATAACAATCACCAAACCAACAAGCACCGGTACCGAGGTGCGTTTTACGACTTCAAACGGCGAGATCTTCGCCATCCCGGCAACCGCAACGACTACGCCAGAAACGGGCGAAAGGGTACGGCCAAGGTTTGACGc                                                                 >  1:4305948/1‑150 (MQ=255)
                                                                                     gCTCTGTAGCAACGATAACAATCACCAAACCAACAAGCACCGGTACCGAGGTGCGTTTTACGACTTCAAACGGCGAGATCTTCGCCATCCCGGCAACCGCAACGACTACGCCAGAAACGGGCGAAAGGGTACGGCCAAGGTTTGACGCCt                                                               <  2:892840/150‑1 (MQ=255)
                                                                                      ctctGTAGCAACGATAACAATCACCAAACCAACAAGCACCGGGACCGAGGTGCGTTTTACTACTTCAAACGGCGAGATCTTCGCCATCCCGGCAACCGCAACGACTACGCCAGAAACGGGCGAAAGGGTACGGCCAAGGTTTGACGCCTg                                                              <  1:2626721/150‑1 (MQ=255)
                                                                                        ctGTAGCAACGATAACAATCACCAAACCAACAAGCACCGGTACCGAGGTGCGTTTTACGACTTCAAACGGCGAGATCTTCGCCATCCCGGCAACCGCAACGACTACGCCAGAAACGGGCGAAAGGGTACGGCCAAGGTTTGACGCCTgca                                                            <  2:4626923/150‑1 (MQ=255)
                                                                                          gTAGCAACGATAACAATCACCAAACCAACAAGCACCGGTACCGAGGTGCGTTTTACTACTTCAAACGGCGAGATCTTCGCCATCCCGGCAACCGCAACGACTACGCCAGAAACGGGCGAAAGGGTACGGCCAAGGTTTGACGCCTgcagc                                                          <  1:619993/150‑1 (MQ=255)
                                                                                          gTAGCAACGATAACAATCACCAAACCAACAAGCACCGGTACCGAGGTGCGTTTTACGACTTCAAACGGCGAGATCTTCGCCATCCCGGCAACCGCAACGACTACGCCAGAAACGGGCGAAAGGGTACGGCCAAGGTTTGACGCCTgcagc                                                          <  2:2558907/150‑1 (MQ=255)
                                                                                              cAACGATAACAATCACCAAACCAACAAGCACCGGTACCGAGGTGCGTTTTACTACTTCAAACGGCGAGATCTTCGCCATCCCGGCAACCGCAACGACTACGCCAGAAACGGGCGAAAGGGTACGGCCAAGGTTTGACGCCTGCAGCATCg                                                      >  2:1389194/1‑150 (MQ=255)
                                                                                               aaCGATAACAATCACCACACCAACCCGCACCGGTACCGCGGTGCGTTTTACGCCTTCAAACGGCGAGATCTTCGCCATCCCGGCAACCGCAACGACTACGCCAGAAACGGGCGAAAGGGTACGGCCAAGGTTTGACGCCTGCAGCATCgg                                                     <  2:4092015/150‑1 (MQ=255)
                                                                                               aaCGATAACAATCACCAAACCAACACGCACCGGTACCGAGGTGCGTTTTACGACTTCAAACGGCGAGATCTTCGCCATCCCGGCAACCGCAACGACTACGCCAGAAACGGGCGAAAGGGTACGGCCAAGGTTTGACGCCTGCAGCATCgg                                                     <  1:4517525/150‑1 (MQ=255)
                                                                                                 cGATAACAATCACCAAACCAACAAGCACCGGTACCGAGGTGCGTTTTACGACTTCAAACGGCGAGATCTTCGCCATCCCGGCAACCGCAACGACTACGCCAGAAACGGGCGAAAGGGTACGGCCAAGGTTTGACGCCTGCAGCATCGGGa                                                   >  1:2945026/1‑150 (MQ=255)
                                                                                                  gATAACAATCACCAAACCAACAAGCACCGGTACCGAGGTGCGTTTAACTACTTAAAACGGCGAGATCTTCTCCAACCCGGCAACCGCAACGACAACGCCAGAAACGTCCGAAAGGGTACTGACAAGGTTTGACGCCTGCAGCATCGGGAt                                                  >  1:808734/1‑150 (MQ=255)
                                                                                                   aTAACAATCACCAAACCAACAAGCACCGGTACCGAGGTGCGTTTTACGACTTCAAACGGCGAGATCTTCGCCATCCCGGCAACCGCAACGACTACGCCAGAAACGGGCGAAAGGGTACGGCCAAGGTTTGACGCCTGCAGCATCGGGATa                                                 >  1:2082661/1‑150 (MQ=255)
                                                                                                   aTAACAATCACCAAACCAACAAGCACCGGTACCGAGGTGCGTTTTACGACTTCAAACGGCGAGATCTTCGCCATCCCGGCAACCGCAACGACTACGCCAGAAACGGGCGAAAGGGTACGGCCAAGGTTTGACGCCTGCAGCATCGGGATa                                                 >  1:2844158/1‑150 (MQ=255)
                                                                                                    tAACAATCACCAAACCAACACGCACCGGTACCGAGGTGCGTTTTACTACTTCAAACGGCGAGATCTTCGCCATCCCGGCAACCGCAACGACTACGCCAGAAACGGGCGAAAGGGTACGGCCAAGGTTTGACGCCTGCAGCATCGGGATAg                                                <  2:1622708/150‑1 (MQ=255)
                                                                                                    tAACAATCACCAAACCAACAAGCACCGGTACCGAGGTGCGTTTTACGACTTCAAACGGCGAGATCTTCGCCATCCCGGCAACCGCAACGACTACGCCAGAAACGGGCGAAAGGGTACGGCCAAGGTTTGACGCCTGCAGCATCGGGATAg                                                >  1:381753/1‑150 (MQ=255)
                                                                                                       cAATCACCAAACCAACAAGCACCGGTACCGAGGTGCGTTTTACGACTTCAAACGGCGAGATCTTCGCCATCCCGGCAACCGCAACGACTACGCCAGAAACGGGCGAAAGGGTACGGCCAAGGTTTGACGCCTGCAGCATCGGGATAGTCa                                             >  2:4680602/1‑150 (MQ=255)
                                                                                                       cAATCACCAAACCAACAAGCACCGGTACCGAGGTGCGTTTTACGACTTCAAACGGCGAGATCTTCGCCATCCCGGCAACCGCAACGACTACGCCAGAAACGGGCGAAAGGGTACGGCCAAGGTTTGACGCCTGCAGCATCGGGATAGTCa                                             >  2:1094195/1‑150 (MQ=255)
                                                                                                         aTCACCAACCCAACACGCACCGGTACCGAGGTGCGTTTTACGACTTCAAACGGCGAGATCTTCGCCATCCCGGCAACCGCAACGACTACGCCAGAAACGGGCGAAAGGGTACGGCCAAGGTTTGACGCCTGCAGCATCGGGATAGTCaaa                                           <  2:644016/150‑1 (MQ=255)
                                                                                                          tCACCAAACCAACAAGCACCGGTACCGAGGTGCGTTTTACGACTTCAAACGGCGAGATCTTCGCCATCCCGGCAACCGCAACGACTACGCCAGAAACGGGCGAAAGGGTACGGCCAAGGTTTGACGCCTGCAGCATCGGGATAGTCAAAt                                          >  1:3538243/1‑150 (MQ=255)
                                                                                                           cACCAAACCAACAAGCACCGGTACCGAGGTGCGTTTTACGACTTCAAACGGCGAGATCTTCGCCATCCCGGCAACCGCAACGACTACGCCAGAAACGGGCGAAAGGGTACGGCCAAGGTTTGACGCCTGCAGCATCGGGATAGTCAAATa                                         <  1:2578162/150‑1 (MQ=255)
                                                                                                           cACCAAACCAACAAGCACCGGTACCGAGGTGCGTTTTACGACTTCAAACGGCGAGATCTTCGCCATCCCGGCAACCGCAACGACTACGCCAGAAACGGGCGAAAGGGTACGGCCAAGGTTTGACGCCTGCAGCATCGGGATAGTCAAATa                                         >  1:3627070/1‑150 (MQ=255)
                                                                                                            accaaaccaaCCAGCACCGGTACCGAGGTGCGTTTTACGACTTCAAACGGCGAGATCTTCGCCATCCCGGCAACCGCAACGACTACGCCAGAAACGGGCGAAAGGGTACGGCCAAGGTTTGACGCCTGCAGCATCGGGATAGTCAAATAc                                        <  2:822633/150‑1 (MQ=255)
                                                                                                            accaaaccaaCAAGCACCGGTACCGAGGTGCGTTTTACTACTTCAAACGGCGAGATCTTCGCCATCCCGGCAACCGCAACGACTACGCCAGAAACGGGCGAAAGGGTACGGCCAAGGTTTGACGCCTGCAGCATCGGGATAGTCAAATAc                                        <  2:1548373/150‑1 (MQ=255)
                                                                                                            accaaaccaaCAAGCACCGGTACCGAGGTGCGTTTTACGACTTCAAACGGCGAGATCTTCGCCATCCCGGCAACCGCAACGACTACGCCAGAAACGGGCGAAAGGGTACGGCCAAGGTTTGACGCCTGCAGCATCGGGATAGTCAAATAc                                        <  1:3012483/150‑1 (MQ=255)
                                                                                                                aaccaaCAAGCACCGGTACCGAGGTGCGTTTTACGACTTCAAACGGCGAGATCTTCGCCATCCCGGCAACCGCAACGACTACGCCAGAAACGGGCGAAAGGGTACGGCCAAGGTTTGACGCCTGCAGCATCGGGATAGTCAAATACGCCg                                    >  2:549568/1‑150 (MQ=255)
                                                                                                                aaccaaCAAGCACCGGTACCGAGGTGCGTTTTACGACTTCAAACGGCGAGATCTTCGCCATCCCGGCAACCGCAACGACTACGCCAGAAACGGGCGAAAGGGTACGGCCAAGGTTTGACGCCTGCAGCATCGGGATAGTCAAATACGCCg                                    <  1:2226344/150‑1 (MQ=255)
                                                                                                                    aacaaGCACCGGTACCGAGGTGCGTTTTACGACTTCAAACGGCGAGATCTTCGCCATCCCGGCAACCGCAACGACTACGCCAGAAACGGGCGAAAGGGTACGGCCAAGGTTTGACGCCTGCAGCATCGGGATAGTCAAATACGCCGGGtt                                >  2:5025133/1‑150 (MQ=255)
                                                                                                                    aacaaGCACCGGTACCGAGGTGCGTTTTACGACTTCAAACGGCGAGATCTTCGCCATCCCGGCAACCGCAACGACTACGCCAGAAACGGGCGAAAGGGTACGGCCAAGGTTTGACGCCTGCAGCATCGGGATAGTCAAATACGCCGGGtt                                >  1:4125172/1‑150 (MQ=255)
                                                                                                                       aaGCACCGGTACCGAGGTGCGTTTTACGACTTCAAACGGCGAGATCTTCGCCATCCCGGCAACCGCAACGACTACGCCAGAAACGGGCGAAAGGGTACGGCCAAGGTTTGACGCCTGCAGCATCGGGATAGTCAAATACGCCGGGTTAAt                             >  2:3613445/1‑150 (MQ=255)
                                                                                                                        aGCACCGGGACCGTGGTGCTTTTTAATACTTCAAACGGCGAGATCTTCGACATCCCGGCAACCGCAACGACTACGCAAGAAACGGGCGAAAGGGTACGGCCAAGGTTTGACGCCTGCAGCATCGGGATAGTCAAATACGCCGGGTTAATg                            <  1:3041167/150‑1 (MQ=255)
                                                                                                                          cACCGGTACCGAGGTGCGTTTTACGACTTCAAACGGCGAGATCTTCGCCATCCCGGCAACCGCAACGACTACGCCAGAAACGGGCGAAAGGGTACGGCCAAGGTTTGACGCCTGCAGCATCGGGATAGTCAAATACGCCGGGTTAATGcc                          <  2:3357053/150‑1 (MQ=255)
                                                                                                                           aCCGGTACCGAGGTGCGTTTTACTACTTCAAACGGCGAGATCTTCGCCATCCCGGCAACCGCAACGACTACGCCAGAAACGGGCGAAAGGGTACGGCCAAGGTTTGACGCCTGCAGCATCGGGATAGTCAAATACGCCGGGTTAATGCCg                         <  1:4378215/150‑1 (MQ=255)
                                                                                                                           aCCGGTACCGAGGTGCGTTTTACTACTTCAAACGGCGAGATCTTCGCCATCCCGGCAACCGCAACGACTACGCCAGAAACGGGCGAAAGGGTACGGCCAAGGTTTGACGCCTGCAGCATCGGGATAGTCAAATACGCCGGGTTAATGCCg                         <  1:1823564/150‑1 (MQ=255)
                                                                                                                           aCCGGTACCGAGGTGCGTTTTACGACTTCAAACGGCGAGATCTTCGCCATCCCGGCAACCGCAACGACTACGCCAGAAACGGGCGAAAGGGTACGGCCAAGGTTTGACGCCTGCAGCATCGGGATAGTCAAATACGCCGGGTTAATGCCg                         <  2:158160/150‑1 (MQ=255)
                                                                                                                              ggTACCGAGGTGCGTTTTACTACTTCAAACGGCGAGATCTTCGCCATCCCGGCAACCGCAACGACTACGCCAGAAACGGGCGAAAGGGTACGGCCAAGGTTTGACGCCTGCAGCATCGGGATAGTCAAATACGCCGGGTTAATGCCGGaa                      >  2:4175022/1‑150 (MQ=255)
                                                                                                                                  ccGAGGTGCGTTTTACGACTTCAAACGGCGAGATCTTCGCCATCCCGGCAACCGCAACGACTACGCCAGAAACGGGCGAAAGGGTACGGCCAAGGTTTGACGCCTGCAGCATCGGGATAGTCAAATACGCCGGGTTAATGCCGGAAGAGt                  >  1:1250065/1‑150 (MQ=255)
                                                                                                                                    gAGGTGCGTTTTACGACTTCAAACGGCGAGATCTTCGCCATCCCGGCAACCGCAACGACTACGCCAGAAACGGGCGAAAGGGTACGGCCAAGGTTTGACGCCTGCAGCATCGGGATAGTCAAATACGCCGGGTTAATGCCGGAAGAGTgc                >  2:1792754/1‑150 (MQ=255)
                                                                                                                                     aGGTGCGTTTTACTACTTCAAACGGCGAGATCTTCGCCATCCCGGCAACCGCAACGACTACGCCAGAAACGGGCGAAAGGGTACGGCCAAGGTTTGACGCCTGCAGCATCGGGATAGTCAAATACGCCGGGTTAATGCCGGAAGAGTgcg               >  1:1551600/1‑150 (MQ=255)
                                                                                                                                     aGGTGCGTTTTACTACTTCAAACGGCGAGATCTTCGCCATCCCGGCAACCGCAACGACTACGCCAGAAACGGGCGAAAGGGTACGGCCAAGGTTTGACGCCTGCAGCATCGGGATAGTCAAATACGCCGGGTTAATGCCGGAAGAGTgcg               >  1:3109590/1‑150 (MQ=255)
                                                                                                                                     aGGTGCGTTTTACGACTTCAAACGGCGAGATCTTCGCCATCCCGGCAACCGCAACGACTACGCCAGAAACGGGCGAAAGGGTACGGCCAAGGTTTGACGCCTGCAGCATCGGGATAGTCAAATACGCCGGGTTAATGCCGGAAGAGTgcg               <  1:291295/150‑1 (MQ=255)
                                                                                                                                      ggTGCGTTTTCCGACTTCAAACGGCGAGATCTTCGCCATCCCGGCAACCGCAACGACTACGCCAGAAACGGGCGAAAGGGTACGGCCAAGGTTTGACGCCTGCAGCATCGGGATAGTCAAATACGCCGGGTTAATGCCGGAAGAGTgcgc              <  2:4868092/150‑1 (MQ=255)
                                                                                                                                        tGCGTTTTACTACTTCAAACGGCGAGATCTTCGCCATCCCGGCAACCGCAACGACTACGCCAGAAACGGGCGAAAGGGTACGGCCAAGGTTTGACGCCTGCAGCATCGGGATAGTCAAATACGCCGGGTTAATGCCGGAAGAGTGCGCCa            <  2:2952652/150‑1 (MQ=255)
                                                                                                                                          cGTTTTACGACTTCAAACGGCGAGATCTTCGCCATCCCGGCAACCGCAACGACTACGCCAGAAACGGGCGAAAGGGTACGGCCAAGGTTTGACGCCTGCAGCATCGGGATAGTCAAATACGCCGGGTTAATGCCGGAAGAGTGCGCCAGt          <  2:2099/150‑1 (MQ=255)
                                                                                                                                           gTTTTACTACTTCAAACGGCGAGATCTTCGCCATCCCGGCAACCGCAACGACTACGCCAGAAACGGGCGAAAGGGTACGGCCAAGGTTTGACGCCTGCAGCATCGGGATAGTCAAATACGCCGGGTTAATGCCGGAAGAGTGCGCCAGtt         >  1:5096557/1‑150 (MQ=255)
                                                                                                                                             tttACGACTTCAAACGGCGAGATCTTCGCCATCCCGGCAACCGCAACGACTACGCCAGAAACGGGCGAAAGGGTACGGCCAAGGTTTGACGCCTGCAGCATCGGGATAGTCAAATACGCCGGGTTAATGCCGGAAGAGTGCGCCAGTTTc       <  1:3850021/150‑1 (MQ=255)
                                                                                                                                               tACGACTTCAAACGGCGAGATCTTCGCCATCCCGGCAACCGCAACGACTACGCCAGAAACGGGCGAAAGGGTACGGCCAAGGTTTGACGCCTGCAGCATCGGGATAGTCAAATACGCCGGGTTAATGCCGGAAGAGTGCGCCAGTTTCgg     >  2:4171602/1‑150 (MQ=255)
                                                                                                                                                  |                                                                                                                                                     
GTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGCTCTGTAGCAACGATAACAATCACCAAACCAACAAGCACCGGTACCGAGGTGCGTTTTACGACTTCAAACGGCGAGATCTTCGCCATCCCGGCAACCGCAACGACTACGCCAGAAACGGGCGAAAGGGTACGGCCAAGGTTTGACGCCTGCAGCATCGGGATAGTCAAATACGCCGGGTTAATGCCGGAAGAGTGCGCCAGTTTCGGGAT  >  CP009273/649995‑650290

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑