Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 702,649 G→T 7.4% P367P (CCG→CCT)  glnS → glutamyl‑tRNA synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273702,6490GT 7.4% 1277.6 / 48.7 334P367P (CCG→CCT) glnSglutamyl‑tRNA synthetase
Reads supporting (aligned to +/- strand):  ref base G (155/154);  new base T (17/8);  total (172/162)
Fisher's exact test for biased strand distribution p-value = 9.85e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 337 total aligned reads displayed.

AAGCAGGACAACACCATTGAGATGGCGTCGCTGGAATCCTGCATCCGTGAAGATCTCAACG‑AAAATGCGCCGCGCGCAATGGCGGTTATCGATCCGGTGAAACTGGTTATCGAAAACTATCAGGGCGAAGGCGAAATGGTTACCATGCCGAACCATCCGAACAAACCGGAAATGGGCAGCCGTCAGGTGCCGTTTAGCGGTGAGATTTGGATTGATCGCGCCGATTTCCGCGAAGAAGCTAACAAGCAGTACAAACGTCTGGTGCTGGGTAAAGAAGTGCGTCTGCGTAATGCTTATGT  >  CP009273/702500‑702798
                                                                                                                                                      |                                                                                                                                                     
aaGCAGGACAACACCATTGAGATGGCGTCGCTGGAATCCTGCATCCGTGAAGATCTCAACG‑AAAATGCGCCGCGCGCAATGGCGGTTATCGATCCGGTGAAACTGGTTATCGAAAACTATCAGGGCGAAGGCGAAATGGTTACCATGCCg                                                                                                                                                       <  2:1860549/150‑1 (MQ=255)
 aGCAGGACAACACCATTGAGATGGCGTCGCTGGAATCCTGCATCCGTGAAGATCTCAACG‑AAAATGCGCCGCGCGCAATGGCGGTTATCGATCCGGTGAAACTGGTTATCGAAAACTATCAGGGCGAAGGCGAAATGGTTACCATGCCGa                                                                                                                                                      >  1:894010/1‑150 (MQ=255)
 aGCAGGACAACACCATTGAGATGGCGTCGCTGGAATCCTGCATCCGTGAAGATCTCAACG‑AAAATGCGCCGCGCGCAATGGCGGTTATCGATCCGGTGAAACTGGGTATCGAAAACTATCAGGGCGAAGGCGAAATGGTTACCATGCCGa                                                                                                                                                      >  2:425058/1‑150 (MQ=255)
     ggACAACACCATTGAGATGGCGTCGCTGGAATCCTGCATCCGTGAAGATCTCAACGAAAAATGCGCCGCGCGCAATGGCGGTTATCGATCCGGTGAAACTGGTTATCGAAAACTATCAGGGCGAAGGCGAAATGGTTACCATGCCGAAcc                                                                                                                                                   >  2:1056671/1‑150 (MQ=255)
       acaacaCCATTGAGATGGCGTCGCTGGAATCCTGCATCCGTGAAGATCTCAACG‑AAAATGCGCCGCGCGCAATGGCGGTTATCGATCCGGTGAAACTGGTTATCGAAAACTATCAGGGCGAAGGCGAAATGGTTACCATGCCGAACCATc                                                                                                                                                >  1:3670035/1‑150 (MQ=255)
        caacaCCATTGAGATGGCGTCGCTGGAATCCTGCATCCGTGAAGATCTCAACG‑AAAATGCGCCGCGCGCAATGGCGGTTATCGATCCGGTGAAACTGGTTATCGAAAACTATCAGGGCGAAGGCGAAATGGTTACCATGCCGAACCATcc                                                                                                                                               <  1:4351549/150‑1 (MQ=255)
          acacCATTGAGATGGCGTCGCTGGAATCCTGCATCCGTGAAGATCTCAACG‑AAAATGCGCCGCGCGCAATGGCGGTTATCGATCCGGTGAAACTTGTTATCGAAAACTATCAGGGCGAAGGCGAAATGGTTACCATGCCGAACCATCCGa                                                                                                                                             >  1:4970550/1‑150 (MQ=255)
          acacCATTGAGATGGCGTCGCTGGAATCCTGCATCCGTGAAGATCTCAACG‑AAAATGCGCCGCGCGCAATGGCGGTTATCGATCCGGTGAAACTGGTTATCGAAAACTATCAGGGCGAAGGCGAAATGGTTACCATGCCGAACCATCCGa                                                                                                                                             <  2:4196601/150‑1 (MQ=255)
             ccATTGAGATGGCGTCGCTGGAATCCTGCATCCGTGAAGATCTCAACG‑AAAATGCGCCGCGCGCAATGGCGGTTATCGATCCGGTGAAACTGGTTATCGAAAACTATCAGGGCGAAGGCGAAATGGTTACCATGCCGAACCATCCGAACa                                                                                                                                          <  1:2804347/150‑1 (MQ=255)
               aTTGAGATGGCGTCGCTGGAATCCTGCATCCGTGAAGATCTCAACG‑AAAATGCGCCGCGCGCAATGGCGGTTATCGATCCGGTGAAACTGGTTATCGAAAACTATCAGGGCGAAGGCGAAATGGTTACCATGCCGAACCATCCGAACaaa                                                                                                                                        <  2:4510263/150‑1 (MQ=255)
                ttGAGATGGCGTCGCTGGAATCCTGCATCCGTGAAGATCTCAACG‑AAAATGCGCCGCGCGCAATGGCGGTTATCGATCCGGTGAAACTGGTTATCGAAAACTATCAGGGCGAAGGCGAAATGGTTACCATGCCGAACCATCCGAACAAAc                                                                                                                                       >  2:4101558/1‑150 (MQ=255)
                  gagaTGGCGTCGCTGGAATCCTGCATCCGTGAAGATCTCAACG‑AAAATGCGCCGCGCGCAATGGCGGTTATCGATCCGGTGAAACTGGTTATCGAAAACTATCAGGGCGAAGGCGAAATGGTTACCATGCCGAACCATCCGAACAAACCg                                                                                                                                     >  1:4107467/1‑150 (MQ=255)
                     aTGGCGTCGCTGGAATCCTGCATCCGTGAAGATCTCAACG‑AAAATGCGCCGCGCGCAATGGCGGTTATCGATCCGGTGAAACTGGTTATCGAAAACTATCAGGGCGAAGGCGAAATGGTTACCATGCCGAACCATCCGAACAAACCGGaa                                                                                                                                  <  2:3649395/150‑1 (MQ=255)
                     aTGGCGTCGCTGGAATCCTGCATCCGTGAAGATCTCAACG‑AAAATGCGCCGCGCGCAATGGCGGTTATCGATCCGGTGAAACTGGTTATCGAAAACTATCAGGGCGAAGGCGAAATGGTTACCATGCCGAACCATCCGAACAAACCGGaa                                                                                                                                  <  2:1981647/150‑1 (MQ=255)
                        gCGTCGCTGGAATCCTGCATCCGTGAAGATCTCAACG‑AAAATGCGCCGCGCGCAATGGCGGTTATCGATCCGGTGAAACTGGTTATCGAAAACTATCAGGGCGAAGGCGAAATGGTTACCATGCCGAACCATCCGAACAAACCGGAAATg                                                                                                                               <  2:1209432/150‑1 (MQ=255)
                        gCGTCGCTGGAATCCTGCATCCGTGAAGATCTCAACG‑AAAATGCGCCGCGCGCAATGGCGGTTATCGATCCGGTGAAACTGGTTATCGAAAACTATCAGGGCGAAGGCGAAATGGTTACCATGCCGAACCATCCGAACAAACCGGAAATg                                                                                                                               <  2:571520/150‑1 (MQ=255)
                          gTCGCTGGAATCCTGCATCCGTGAAGATCTCAACG‑AAAATGCGCCGCGCGCAATGGCGGTTATCGATCCGGTGAAACTGGTTATCGAAAACTATCAGGGCGAAGGCGAAATGGTTACCATGCCGAACCATCCGAACAAACCGGAAATggg                                                                                                                             >  2:2599608/1‑150 (MQ=255)
                            cGCTGGAATCCTGCATCCGTGAAGATCTCAACG‑AAAATGCGCCGCGCGCAATGGCGGTTATCGATCCGGTGAAACTGGTTATCGAAAACTATCAGGGCGAAGGCGAAATGGTTACCATGCCGAACCATCCGAACAAACCGGAAATGGGCa                                                                                                                           <  1:3478805/150‑1 (MQ=255)
                             gCTGGAATCCTGCATCCGTGAAGATCTCAACG‑AAAATGCGCCGCGCGCAATGGCGGTTATCGATCCGGTGAAACTGGTTATCGAAAACTATCAGGGCGAAGGCGAAATGGTTACCATGCCGAACCATCCGAACAAACCGGAAATGGGCAg                                                                                                                          >  2:4685111/1‑150 (MQ=255)
                               tGGAATCCTGCATCCGTGAAGATCTCAACG‑AAAATGCGCCGCGCGCAATGGCGGTTATCGATCCGGTGAAACTGGTTATCGAAAACTATCAGGGCGAAGGCGAAATGGTTA‑CATGCCTAACCATCCGAACAAACCGGAAATGGGCAGCCg                                                                                                                       >  2:2661004/1‑150 (MQ=255)
                                ggAATCCTGCATCCGTGAAGATCTCAACG‑AAAATGCGCCGCGCGCAATGGCGGTTATCGATCCGGTGAAACTGGTTATCGAAAACTATCAGGGCGAAGGCGAAATGGTTACCATGCCGAACCATCCGAACAAACCGGACATGGGCAGCCg                                                                                                                       >  2:3825271/1‑150 (MQ=255)
                                ggAATCCTGCATCCGTGAAGATCTCAACG‑AAAATGCGCCGCGCGCAATGGCGGTTATCGATCCGGTGAAACTGGTTATCGAAAACTATCAGGGCGAAGGCGAAATGGTTACCATGCCGAACCATCCGAACAAACCGGAAATGGGCAGCCg                                                                                                                       >  2:1833054/1‑150 (MQ=255)
                                  aaTCCTGCATCCGTGAAGATCTCAACG‑AAAATGCGCCGCGCGCAATGGCGGTTATCGATCCGGTGAAACTGGTTATCGAAAACTATCAGGGCGAAGGCGAAATGGTTACCATGCCGAACCATCCGAACAAACCGGAAATGGGCAGCCGTc                                                                                                                     >  1:3156104/1‑150 (MQ=255)
                                  aaTCCTGCATCCGTGAAGATCTCAACG‑AAAATGCGCCGCGCGCAATGGCGGTTATCGATCCGGTGAAACTGGTTATCGAAAACTATCAGGGCGAAGGCGAAATGGTTACCATGCCGAACCATCCGAACAAACCGGAAATGGGCAGCCGTc                                                                                                                     >  1:3318506/1‑150 (MQ=255)
                                       tGCATCCGTGAAGATCTCAACG‑AAAATGCGCCGCGCGCAATGGCGGTTATCGATCCGGTGAAACTGGTTATCGAAAACTATCAGGGCGAAGGCGAAATGGTTACCATGCCGAACCATCCGAACAAACCGGAAATGGGCAGCCGTCAGGTg                                                                                                                <  1:2968857/150‑1 (MQ=255)
                                          aTCCGTGAAGATCTCAACG‑AAAATGCGCCGCGCGCAATGGCGGTTATCGATCCGGTGAAACTGGTTATCGAAAACTATCAGGGCGAAGGCGAAATGGTTACCATGCCGAACCATCCGAACAAACCGGAAATGGGCAGCCGTCAGGTGCCg                                                                                                             >  1:2107896/1‑150 (MQ=255)
                                           tCCGTGAAGATCTCAACG‑AAAATGCGCCGCGCGCAATGGCGGTTATCGATCCGGTGAAACTGGTTATCGAAAACTATCAGGGCGAAGGCGAAATGGTTACCATGCCGAACCATCCGAACAAACCGGAAATGGGCAGCCGTCAGGTGCCGt                                                                                                            <  1:1858118/150‑1 (MQ=255)
                                              gTGAAGATCTCAACG‑AAAATGCGCCGCGCGCAATGGCGGTTATCGATCCGGTGAAACTGGTTATCGAAAACTATCAGGGCGAAGGCGAAATGGTTACCATGCCGAACCATCCGAACAAACCGGAAATGGGCAGCCGTCAGGTGCCGTTTa                                                                                                         <  2:1724166/150‑1 (MQ=255)
                                                gAAGATCTCAACG‑AAAATGCGCCGCGCGCAATGGCGGTTATCGATCCGGTGAAACTGGTTATCGAAAACTATCAGGGCGAAGGCGAAATGGTTACCATGCCGAACCATCCGAACAAACCGGAAATGGGCAGCCGTCAGGTGCCGTTTAGc                                                                                                       >  1:2184405/1‑150 (MQ=255)
                                                gAAGATCTCAACG‑AAAATGCGCCGCGCGCAATGGCGGTTATCGATCCGGTGAAACTGGTTATCGAAAACTATCAGGGCGAAGGCGAAATGGTTACCATGCCGAACCATCCGAACAAACCGGAAATGGGCAGCCGTCAGGTGCCGTTTAGc                                                                                                       >  2:1079641/1‑150 (MQ=255)
                                                 aaGATCTCAACG‑AAAATGCGCCGCGCGCAATGGCGGTTATCGATCCGGTGAAACTGGTTATCGAAAACTATCAGGGCGAAGGCGAAATGGTTACCATGCCGAACCATCCGAACAAACCGGAACTGGGCAGCCGTCAGGTGCCGTTTAGCg                                                                                                      >  2:88630/1‑150 (MQ=255)
                                                    aTCTCAACG‑AAAATGCGCCGCGCGCAATGGCGGTTATCGATCCGGTGAAACTGGTTATCGAAAACTATCAGGGCGAAGGCGAAATGGTTACCATGCCGAACCATCCGAACAAACCGGAAATGGGCAGCCGTCAGGTGCCGTTTAGCGGTg                                                                                                   >  1:2455257/1‑150 (MQ=255)
                                                     tctcAACG‑AAAATGCGCCGCGCGCAATGGCGGTTATCGATCCGGTGAAACTGGTTATCGAAAACTATCAGGGCGAAGGCGAAATGGTTACCATGCCTAACCATCCGAACAAACCGGAAATGGGCAGCCGTCAGGTGCCGTTTAGCGGTga                                                                                                  <  1:1321627/150‑1 (MQ=255)
                                                       tcAACG‑AAAATGCGCCGCGCGCAATGGCGGTTATCGATCCGGTGAAACTGGTTATCGAAAACTATCAGGGCGAAGGCGAAATGGTTACCATGCCGAACCATCCGAACAAACCGGAAATGGGCAGCCGTCAGGTGCCGTTTAGCGGTgaga                                                                                                <  1:5035993/150‑1 (MQ=255)
                                                        cAACG‑AAAATGCGCCGCGCGCAATGGCGGTTATCGATCCGGTGAAACTGGTTATCGAAAACTATCAGGGCGAAGGCGAAATGGTTACCATGCCGAACCATCCGAACAAACCGGAAATGGGCAGCCGTCAGGTGCCGTTTAGCGGTGAGAt                                                                                               <  1:1806156/150‑1 (MQ=255)
                                                         aaCG‑AAAATGCGCCGCGCGCAATGGCGGTTATCGATCCGGTGAAACTGGTTATCGAAAACTATCAGGGCGAAGGCGAAATGGTTACCATGCCGAACCATCCGAACAAACCGGAAATGGGCAGCCGTCAGGTGCCGTTTAGCGGTGAGAtt                                                                                              >  2:2196014/1‑150 (MQ=255)
                                                          aCG‑AAAATGCGCCGCGCGCAATGGCGGTTATCGATCCGGTGAAACTGGTTATCGAAAACTATCAGGGCGAAGGCGAAATGGTTACCATGCCTAACCATCCGAACAAACCGGAAATGGGCAGCCGTCAGGTGCCGTTTAGCGGTGAGAttt                                                                                             <  2:1469899/150‑1 (MQ=255)
                                                           cG‑AAAATGCGCCGCGCGCAATGGCGGTTATCGATCCGGTGAAACTGGTTATCGAAAACTATCAGGGCGAAGGCGAAATGGTTACCATGCCGAACCATCCGAACAAACCGGAAATGGGCAGCCGTCAGGTGCCGTTTAGCGGTGAGATTTg                                                                                            <  2:3322066/150‑1 (MQ=255)
                                                            g‑AAAATGCGCCGCGCGCAATGGCGGTTATCGATCCGGTGAAACTGGTTATCGAAAACTATCAGGGCGAAGGCGAAATGGTTACCATGCCGAACCATCCGAACAAACCGGAAATGGGCAGCCGTCAGGTGCCGTTTAGCGGTGAGATTTgg                                                                                           >  2:1940553/1‑150 (MQ=255)
                                                            g‑AAAATGCGCCGCGCGCAATGGCGGTTATCGATCCGGTGAAACTGGTTATCGAAAACTATCAGGGCGAAGGCGAAATGGTTACCATGCCGAACCATCCGAACAAACCGGAAATGGGCAGCCGTCAGGTGCCGTTTAGCGGGGAGATTTgg                                                                                           >  1:4773730/1‑150 (MQ=255)
                                                              aaaaTGCGCCGCGCGCAATGGCGGTTATCGATCCGGTGAAACTGGTTATCGAAAACTATCAGGGCGAAGGCGAAATGGTTACCATGCCGAACCATCCGAACAAACCGGAAATGGGCAGCCGTCAGGTGCCGTTTAGCGGTGAGATTTGGa                                                                                          <  1:4008934/150‑1 (MQ=255)
                                                               aaaTGCGCCGCGCGCAATGGCGGTTATCGATCCGGTGAAACTGGTTATCGAAAACTATCAGGGCGAAGGCGAAATGGTTACCATGCCGAACCATCCGAACAAACCGGAAATGGGCAGCCGTCAGGTGCCGTTTAGCGGTGAGATTTGGAt                                                                                         >  1:2047952/1‑150 (MQ=255)
                                                                 aTGCGCCGCGCGCAATGGCGGTTATCGATCCGGTGAAACTGGTTATCGAAAACTATCAGGGCGAAGGCGAAATGGTTACCATGCCTAACCATCCGAACAAACCGGAAATGGGCAGCCGTCAGGTGCCGTTTAGCGGTGAGATTTGGATTg                                                                                       >  1:2258756/1‑150 (MQ=255)
                                                                 aTGCGCCGCGCGCAATGGCGGTTATCGATCCGGTGAAACTGGTTATCGAAAACTATCAGGGCGAAGGCGAAATGGTTACCATGCCGAACCATCCGAACAAACCGGAAATGGGCAGCCGTCAGGTGCCGTTTAGCGGTGAGATTTGGATTg                                                                                       <  2:4239246/150‑1 (MQ=255)
                                                                     gccgcGCGCAATGGCGGTTATCGATCCGGTGAAACTGGTTATCGAAAACTATCAGGGCGAAGGCGAAATGGTTACCATGCCGAACCATCCGAACAAACCGGAAATGGGCAGCCGTCAGGTGCCGTTTAGCGGTGCGATTTGGATTGATcg                                                                                   >  1:3268210/1‑150 (MQ=255)
                                                                        gcgcgcAATGGCGGTTATCGATCCGGTGAAACTGGTTATCGAAAACTATCAGGGCGAAGGCGAAATGGTTACCATGCCGAACCATCCGAACAAACCGGAAATGGGCAGCCGTCAGGTGCCGTTTAGCGGTGAGATTTGGATTGATCgcgc                                                                                >  1:2446580/1‑150 (MQ=255)
                                                                         cgcgcAATGGCGGTTATCGATCCGGTGAAACTGGTTATCGAAAACTATCAGGGCGAAGGCGAAATGGTTACCATGCCGAACCATCCGAACAAACCGGAAATGGGCAGCCGTCAGGTGCCGTTTAGCGGTGAGATTTGGATTGATCGCGcc                                                                               >  2:4153234/1‑150 (MQ=255)
                                                                          gcgcAATGGCGGTTATCGATCCGGTGAAACTGGTTATCGAAAACTATCAGGGCGAAGGCGAAATGGTTACCATGCCGAACCATCCGAACAAACCGGAAATGGGCAGCCGTCAGGTGCCGTTTAGCGGTGAGATTTGGATTGATCGCGCCg                                                                              <  2:445960/150‑1 (MQ=255)
                                                                           cgcAATGGCGGTTATCGATCCGGTGAAACTGGTTATCGAAAACTATCAGGGCGAAGGCGAAATGGTTACCATGCCGAACCATCCGAACAAACCGGAAATGGGCAGCCGTCAGGTGCCGTTTAGCGGTGAGATTTGGATTGATCGCGCCGa                                                                             >  2:165205/1‑150 (MQ=255)
                                                                           cgcAATGGCGGTTATCGATCCGGTGAAACTGGTTATCGAAAACTATCAGGGCGAAGGCGAAATGGTTACCATGCCGAACCATCCGAACAAACCGGAAATGGGCAGCCGTCAGGTGCCGTTTAGCGGTGAGATTTGGATTGATCGCGCCGa                                                                             >  1:4574716/1‑150 (MQ=255)
                                                                            gcAATGGCGGTTATCGATCCGGTGAAACTGGTTATCGAAAACTATCAGGGCGAAGGCGAAATGGTTACCATGCCGAACCATCCGAACAAACCGGAAATGGGCAGCCGTCAGGTGCCGTTTAGCGGTGAGATTTGGATTGATCGCGCCGAt                                                                            >  1:2866904/1‑150 (MQ=255)
                                                                                tGGCGGTTATCGATCCGGTGAAACTGGTTATCGAAAACTATCAGGGCGAAGGCGAAATGGTTACCATGCCGAACCATCCGAACAAACCGGAAATGGGCAGCCGTCAGGTGCCGTTTAGCGGTGAGATTTGGATTGATCGCGCCGATTTcc                                                                        <  1:575105/150‑1 (MQ=255)
                                                                                tGGCGGTTATCGATCCGGTGAAACTGGTTATCGAAAACTATCAGGGCGAAGGCGAAATGGTTACCATGCCGAACCATCCGAACAAACCGGAAATGGGCAGCCGTCAGGTGCCGTTTAGCGGTGAGATTTGGATTGATCGCGCCGATTTcc                                                                        <  2:3074860/150‑1 (MQ=255)
                                                                                 ggCGGTTATCGATCCGGTGAAACTGGTTATCGAAAACTATCAGGGCGAAGGCGAAATGGTTACCATGCCGAACCATCCGAACAAACCGGAAATGGGCAGCCGTCAGGTGCCGTTTAGCGGTGAGATTTGGATTGATCGCGCCGATTTCcg                                                                       <  2:3194601/150‑1 (MQ=255)
                                                                                    ggTTATCGATCCGGTGAAACTGGTTATCGAAAACTATCAGGGCGAAGGCGAAATGGTTACCATGCCGAACCATCCGAACAAACCGGAAATGGGCAGCCGTCAGGTGCCGTTTAGCGGTGAGATTTGGATTGATCGCGCCGATTTCCGCga                                                                    >  2:2413412/1‑150 (MQ=255)
                                                                                     gTTATCGATCCGGTGAAACTGGTTATCGAAAACTATCAGGGCGAAGGCGAAATGGTTACCATGCCGAACCATCCGAACAAACCGGAAATGGGCAGCCGTCAGGTGCCGTTTAGCGGTGAGATTTGGATTGATCGCGCCGATTTCCGCgaa                                                                   <  1:4454865/150‑1 (MQ=255)
                                                                                          cGATCCGGTGAAACTGGTTATCGAAAACTATCAGGGCGAAGGCGAAATGGTTACCATGCCGAACCATCCGAACAAACCGGAAATGGGCAGCCGTCAGGTGCCGTTTAGCGGTGAGATTTGGATTGATCGCGCCGATTTCCGCGAAGAAGc                                                              <  1:3716427/150‑1 (MQ=255)
                                                                                             tCCGGTGAAACTGGTTATCGAAAACTATCAGGGCGAAGGCGAAATGGTTACCATGCCGAACCATCCGAACAAACCGGAAATGGGCAGCCGTCAGGTGCCGTTTAGCGGTGAGATTTGGATTGATCGCGCCGATTTCCGCGAAGAAGCTaa                                                           <  1:3623205/150‑1 (MQ=255)
                                                                                               cGGTGAAACTGGTTATCGAAAACTATCAGGGCGAAGGCGAAATGGTTACCATGCCGAACCATCCGAACAAACCGGAAATGGGCAGCCGTCAGGTGCCGTTTAGCGGTGAGATTTGGATTGATCGCGCCGATTTCCGCGAAGAAGCTAACa                                                         >  1:2674972/1‑150 (MQ=255)
                                                                                                 gTGAAACTGGTTATCGAAAACTATCAGGGCGAAGGCGAAATGGTTACCATGCCGAACCATCCGAACAAACCGGAAATGGGCAGCCGTCAGGTGCCGTTTAGCGGTGAGATTTGGATTGATCGCGCCGATTTCCGCGAAGAAGCTAACAAg                                                       >  1:1684462/1‑150 (MQ=255)
                                                                                                 gTGAAACTGGTTATCGAAAACTATCAGGGCGAAGGCGAAATGGTTACCATGCCGAACCATCCGAACAAACCGGAAATGGGCAGCCGTCAGGTGCCGTTTAGCGGTGAGATTTGGATTGATCGCGCCGATTTCCGCGAAGAAGCTAACAAg                                                       <  2:4311851/150‑1 (MQ=255)
                                                                                                     aaCTGGTTATCGAAAACTATCAGGGCGAAGGCGAAATGGTTACCATGCCGAACCATCCGAACAAACCGGAAATGGGCAGCCGTCAGGTGCCGTTTAGCGGTGAGATTTGGATTGATCGCGCCGATTTCCGCGAAGAAGCTAACAAGCAGt                                                   <  2:812445/150‑1 (MQ=255)
                                                                                                       cTGGTTATCGAAAACTATCAGGGCGAAGGCGAAATGGTTACCATGCCGAACCATCCGAACAAACCGGAAATGGGCAGCCGTCAGGTGCCGTTTAGCGGTGAGATTTGGATTGATCGCGCCGATTTCCGCGAAGAAGCTAACAAGCAGTAc                                                 >  2:2067395/1‑150 (MQ=255)
                                                                                                        tGGTTATCGAAAACTATCAGGGCGAAGGCGAAATGGTTACCATGCCTAACCATCCGAACAAACCGGAAATGGGCAGCCGTCAGGTGCCGTTTAGCGGTGAGATTTGGATTGATCGCGCCGATTTCCGCGAAGAAGCTAACAAGCAGTACa                                                >  2:2827502/1‑150 (MQ=255)
                                                                                                         ggTTATCGAAAACTATCAGGGCGAAGGCGAAATGGTTACCATGCCGAACCATCCGAACAAACCGGAAATGGGCAGCCGTCAGGTGCCGTTTAGCGGTGAGATTTGGATTGATCGCGCCGATTTCCGCGAAGAAGCTAACAAGCAGTACaa                                               >  2:2383421/1‑150 (MQ=255)
                                                                                                           ttATCGAAAACTATCAGGGCGAAGGCGAAATGGTTACCATGCCGAACCATCCGAACAAACCGGAAATGGGCAGCCGTCAGGTGCCGTTTAGCGGTGAGATTTGGATTGATCGCGCCGATTTCCGCGAAGAAGCTAACAAGCAGTACAAAc                                             >  1:1484860/1‑150 (MQ=255)
                                                                                                            tATCGAAAACTATCAGGGCGAAGGCGAAATGGTTACCATGCCGAACCATCCGAACAAACCGGAAATGGGCAGCCGTCAGGTGCCGTTTAGCGGTGAGATTTGGATTGATCGCGCCGATTTCCGCGAAGAAGCTAACAAGCAGTACAAACg                                            <  1:1040229/150‑1 (MQ=255)
                                                                                                            tATCGAAAACTATCAGGGCGAAGGCGAAATGGTTACCATGCCGAACCATCCGAACAAACCGGAAATGGGCAGCCGTCAGGTGCCGTTTAGCGGTGAGATTTGGATTGATCGCGCCGATTTCCGCGAAGAAGCTAACAAGCAGTACAAACg                                            <  1:4426947/150‑1 (MQ=255)
                                                                                                                gAAAACTATCAGGGCGAAGGCGAAATGGTTACCATGCCGAACCATCCGAACAAACCGGAAATGGGCAGCCGTCAGGTGCCGTTTAGCGGTGAGATTTGGATTGATCGCGCCGATTTCCGCGAAGAAGCTAACAAGCAGTACAAACGTCTg                                        >  1:1188253/1‑150 (MQ=255)
                                                                                                                 aaaaCTATCAGGGCGAAGGCGAAATGGTTACCATGCCGAACCATCCGAACAAACCGGAAATGGGCAGCCGTCAGGTGCCGTTTAGCGGTGAGATTTGGATTGATCGCGCCGATTTCCGCGAAGAAGCTAACAAGCAGTACAAACGTCTgg                                       >  1:1542620/1‑150 (MQ=255)
                                                                                                                  aaaCTATCAGGGCGAAGGCGAAATGGTTACCATGCCGAACCATCCGAACAAACCGGACATGGGCAGCCGTCAGGTGCCGTTTAGCGGTGAGAGTGGGAGTGATCGCGCCGATTTCCGCGCCGACGCTAACCCGCAGTACAAACggcgggg                                      >  2:3463378/1‑144 (MQ=255)
                                                                                                                    aCTATCAGGGCGAAGGCGAAATGGTTACCATGCCTAACCATCCGAACAAACCGGAAATGGGCAGCCGTCAGGTGCCGTTTAGCGGTGAGATTTGGATTGATCGCGCCGATTTCCGCGAAGAAGCTAACAAGCAGTACAAACGTCTGGTGc                                    >  2:2197671/1‑150 (MQ=255)
                                                                                                                       aTCAGGGCGAAGGCGAAATGGTTACCATGCCGAACCATCCGAACAAACCGGAAATGGGCAGCCGTCAGGTGCCGTTTAGCGGTGAGATTTGGATTGATCGCGCCGATTTCCGCGAAGAAGCTAACAAGCAGTACAAACGTCTGGTGCTgg                                 <  1:3933959/150‑1 (MQ=255)
                                                                                                                        tCAGGGCGAAGGCGAACTGGGTACCATGCCGACCCATCCGCACACACCGGAACTGGGCAGCCGTCAGGTGCCGTTTCGCGGTGAGATTTGGATTGATCGCGCCGATTTCCGCGAAGAAGCTAACAAGCAGTACAAACGTCTGGTGCTggg                                <  2:4489933/150‑1 (MQ=255)
                                                                                                                        tCAGGGCGAAGGCGAAATGGTTACCATGCCGAACCATCCGAACAAACCGGAAATGGGCAGCCGTCAGGTGCCGTTTAGCGGTGAGATTTGGATTGATCGCGCCGATTTCCGCGAAGAAGCTAACAAGCAGTACAAACGTCTGGTGCTggg                                >  1:3340071/1‑150 (MQ=255)
                                                                                                                         cAGGGCGAAGGCGAAATGGTTACCATGCCGAACCATCCGAACAAACCGGAAATGGGCAGCCGTCAGGTGCCGTTTAGCGGTGAGATTTGGATTGATCGCGCCGATTTCCGCGAAGAAGCTAACAAGCAGTACAAACGTCTGGTGCTGGGt                               >  2:2735227/1‑150 (MQ=255)
                                                                                                                         cAGGGCGAAGGCGAAATGGTTACCATGCCGAACCATCCGAACAAACCGGAAATGGGCAGCCGTCAGGTGCCGTTTAGCGGTGAGATTTGGATTGATCGCGCCGATTTCCGCGAAGAAGCTAACAAGCAGTACAAACGTCTGGTGCTGGGt                               <  1:2518927/150‑1 (MQ=255)
                                                                                                                           gggCGAAGGCGAAACGGTTACCATGCCGAACCATCCGAACAAACCGGAAATGGGCAGCCGTCAGGTGCCGTTTAGCGGTGAGATTTGGATTGATCGCGCCGATTTCCGCGAAGAAGCTAACAAGCAGTACAAACGTCTGGTGCTGGGTaa                             <  2:1803333/150‑1 (MQ=255)
                                                                                                                             gcgaaggcgaaATGGTTACCATGCCGAACCATCCGAACAAACCGGAAATGGGCAGCCGTCAGGTGCCGTTTAGCGGTGAGATTTGGATTGATCGCGCCGATTTCCGCGAAGAAGCTAACAAGCAGTACAAACGTCTGGTGCTGGGTAaag                           <  1:2380857/150‑1 (MQ=255)
                                                                                                                             gcgaaggcgaaATGGTTACCATGCCGAACCATCCGAACAAACCGGAAATGGGCAGCCGTCAGGTGCCGTTTAGCGGTGAGATTTGGATTGATCGCGCCGATTTCCGCGAAGAAGCTAACAAGCAGTACAAACGTCTGGTGCTGGGTAaag                           >  2:3859462/1‑150 (MQ=255)
                                                                                                                              cgaaggcgaaATGGTTACCATGCCGAACCATCCGAACAAACCGGAAATGGGCAGCCGTCAGGTGCCGTTTAGCGGTGAGATTTGGATTGATCGCGCCGATTTCCGCGAAGAAGCTAACAAGCAGTACAAACGTCTGGTGCTGGGTAaaga                          >  2:3785085/1‑150 (MQ=255)
                                                                                                                               gaaggcgaaATGGTTACCATGCCGAACCATCCGAACAAACCGGAAATGGGCAGCCGTCAGGTGCCGTTTAGCGGTGAGATTTGGATTGATCGCGCCGATTTCCGCGAAGAAGCTAACAAGCAGTACAAACGTCTGGTGCTGGGTAaagaa                         >  1:1178970/1‑150 (MQ=255)
                                                                                                                               gaaggcgaaATGGTTACCATGCCGAACCATCCGAACAAACCGGAAATGGGCAGCCGTCAGGTGCCGTTTAGCGGTGAGATTTGGATTGATCGCGCCGATTTCCGCGAAGAAGCTAACAAGCAGTACAAACGTCTGGTGCTGGGTAaagaa                         <  1:4162225/150‑1 (MQ=255)
                                                                                                                                aaggcgaaATGGTTACCATGCCGAACCATCCGAACAAACCGGAAATGGGCAGCCGTCAGGTGCCGTTTAGCGGTGAGATTTGGATTGATCGCGCCGATTTCCGCGAAGAAGCTAACAAGCAGTACAAACGTCTGGTGCTGGGTAaagaag                        <  2:1288274/150‑1 (MQ=255)
                                                                                                                                aaggcgaaATGGTTACCATGCCGAACCATCCGAACAAACCGGAAATGGGCAGCCGTCAGGTGCCGTTTAGCGGTGAGATTTGGATTGATCGCGCCGATTTCCGCGAAGAAGCTAACAAGCAGTACAAACGTCTGGTGCTGGGTAaagaag                        <  2:1601248/150‑1 (MQ=255)
                                                                                                                                 aggcgaaATGGTTACCATGCCGAACCATCCGAACAAACCGGAAATGGGCAGCCGTCAGGTGCCGTTTAGCGGTGAGATTTGGATTGATCGCGCCGATTTCCGCGAAGAAGCTAACAAGCAGTACAAACGTCTGGTGCTGGGTAAAGAAGt                       <  2:4880263/150‑1 (MQ=255)
                                                                                                                                  ggcgaaATGGTTACCATGCCGAACCATCCGAACAAACCGGAAATGGGCAGCCGTCAGGTGCCGTTTAGCGGTGAGATTTGGATTGATCGCGCCGATTTCCGCGAAGAAGCTAACAAGCAGTACAAACGTCTGGTGCTGGGTAAAGAAGTg                      <  1:420630/150‑1 (MQ=255)
                                                                                                                                    cgaaATGGTGACCATGCCGAACCATCCGAACAAACCGGAAATGGGCAGCCGTCAGGTGCCGTTTAGCGGTGAGATTTGGATTGATCGCGCCGATTTCCGCGAAGAAGCTAACAAGCAGTACAAACGTCTGGTGCTGGGTAAAGAAGTGCg                    <  2:2881666/150‑1 (MQ=255)
                                                                                                                                        aTGGTTACCATGCCGAACCATCCGAACAAACCGGAAATGGGCAGCCGTCAGGTGCCGTTTAGCGGTGAGATTTGGATTGATCGCGCCGATTTCCGCGAAGAAGCTAACAAGCAGTACAAACGTCTGGTGCTGGGTAAAGAAGTGCGTCTg                <  2:2794982/150‑1 (MQ=255)
                                                                                                                                         tGGTTACCATGCCGAACCATCCGAACAAACCGGAAATGGGCAGCCGTCAGGTGCCGTTTAGCGGTGAGATTTGGATTGATCGCGCCGATTTCCGCGAAGAAGCTAACAAGCAGTACAAACGTCTGGTGCTGGGTAAAGAAGTGCGTCTGc               >  1:2256398/1‑150 (MQ=255)
                                                                                                                                         tGGTTACCATGCCGAACCATCCGAACAAACCGGAAATGGGCAGCCGTCAGGTGCCGTTTAGCGGTGAGATTTGGATTGATCGCGCCGATTTCCGCGAAGAAGCTAACAAGCAGTACAAACGTCTGGTGCTGGGTAAAGAAGTGCGTCTGc               <  1:639073/150‑1 (MQ=255)
                                                                                                                                          ggTTACCATGCCTAACCATCCGAACAAACCGGAAATGGGCAGCCGTCAGGTGCCGTTTAGCGGTGAGATTTGGATTGATCGCGCCGATTTCCGCGAAGAAGCTAACAAGCAGTACAAACGTCTGGTGCTGGGTAAAGAAGTGCGTCTGCg              >  2:675234/1‑150 (MQ=255)
                                                                                                                                          ggTTACCATGCCGAACCATCCGAACAAACCGGAAATGGGCAGCCGTCAGGTGCCGTTTAGCGGTGAGATTTGGATTGATCGCGCCGATTTCCGCGAAGAAGCTAACAAGCAGTACAAACGTCTGGTGCTGGGTAAAGAAGTGCGTCTGCg              >  2:4679999/1‑150 (MQ=255)
                                                                                                                                           gTTACCATGCCGAACCATCCGAACAAACCGGAAATGGGCAGCCGTCAGGTGCCGTTTAGCGGTGAGATTTGGATTGATCGCGCCGATTTCCGCGAAGAAGCTAACAAGCAGTACAAACGTCTGGTGCTGGGTAAAGAAGTGCGTCTGCGt             <  1:1890296/150‑1 (MQ=255)
                                                                                                                                            ttACCATGCCGAACCATCCGAACAAACCGGAAATGGGCAGCCGTCAGGTGCCGTTTAGCGGTGAGATTTGGATTGATCGCGCCGATTTCCGCGAAGAAGCTAACAAGCAGTACAAACGTCTGGTGCTGGGTAAAGAAGTGCGTCTGCGTa            >  2:1133625/1‑150 (MQ=255)
                                                                                                                                               ccATGCCGAACCATCCGAACAAACCGGAAATGGGCAGCCGTCAGGTGCCGTTTAGCGGTGAGATTTGGATTGATCGCGCCGATTTCCGCGAAGAAGCTAACAAGCAGTACAAACGTCTGGTGCTGGGTAAAGAAGTGCGTCTGCGTAATg         >  2:1516213/1‑150 (MQ=255)
                                                                                                                                               ccATGCCGAACCATCCGAACAAACCGGAAATGGGCAGCCGTCAGGTGCCGTTTAGCGGTGAGATTTGGATTGATCGCGCCGATTTCCGCGAAGAAGCTAACAAGCAGTACAAACGTCTGGTGCTGGGTAAAGAAGTGCGTCTGCGTAATg         <  1:1062064/150‑1 (MQ=255)
                                                                                                                                               ccATGCCGAACCATCCGAACAAACCGGAAATGGGCAGCCGTCAGGTGCCGTTTAGCGGTGAGAGTTGGATTGATCGCGCCGATTTCCGCGAAGAAGCTAACAAGCAGTACAAACGTCTGGTGCTGGGTAAAGAAGTGCGTCTGCGTACTg         >  1:5104288/1‑150 (MQ=255)
                                                                                                                                                   gCCGAACCATCCGAACAAACCGGAAATGGGCAGCCGTCAGGTGCCGTTTAGCGGTGAGATTTGGATTGATCGCGCCGATTTCCGCGAAGAAGCTAACAAGCAGTACAAACGTCTGGTGCTGGGTAAAGAAGTGCGTCTGCGTAATGCTTa     <  1:4700331/150‑1 (MQ=255)
                                                                                                                                                     cGAACCATCCGAACAAACCGGAAATGGGCAGCCGTCAGGTGCCGTTTAGCGGTGAGATTTGGATTGATCGCGCCGATTTCCGCGAAGAAGCTAACAAGCAGTACAAACGTCTGGTGCTGGGTCAAGAAGTGCGTCTGCGGAATGCTTAtg   >  1:128234/1‑150 (MQ=255)
                                                                                                                                                      |                                                                                                                                                     
AAGCAGGACAACACCATTGAGATGGCGTCGCTGGAATCCTGCATCCGTGAAGATCTCAACG‑AAAATGCGCCGCGCGCAATGGCGGTTATCGATCCGGTGAAACTGGTTATCGAAAACTATCAGGGCGAAGGCGAAATGGTTACCATGCCGAACCATCCGAACAAACCGGAAATGGGCAGCCGTCAGGTGCCGTTTAGCGGTGAGATTTGGATTGATCGCGCCGATTTCCGCGAAGAAGCTAACAAGCAGTACAAACGTCTGGTGCTGGGTAAAGAAGTGCGTCTGCGTAATGCTTATGT  >  CP009273/702500‑702798

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑