BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	734,870	C→A	7.5%	H138Q (CAC→CAA)	ybgA →	DUF1722 family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	734,870	0	C	A	7.5%	1162.6 / 43.7	305	H138Q (CAC→CAA)	ybgA	DUF1722 family protein
Reads supporting (aligned to +/- strand): ref base C (137/145); new base A (13/10); total (150/155)
Fisher's exact test for biased strand distribution p-value = 5.20e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.91e-01

Only 100 of 325 total aligned reads displayed.

CCGCGCGATCACACCAATGTTTTGATGCACGTTCAGGGTTATTTTCGCCCGCATATTGATTCCACAGAACGCCAGCAGCTGGCTGCGCTTATCGACAGTTATCGCCGTGGCGAGCAACCACTTCTTGCGCCGCTGATGCGTATCAAACACTATATGGCGCTTTATCCTGACGCCTGGCTTTCAGGGCAGCGTTATTTCGAACTTTGGCCGCGTGTGATTAACTTGCGCCATTCAGGAGTTTTATGACTACCCATCTGGTCTGGTTTCGCCAGGATTTACGTCTGCACGATAATCTCGCA  >  CP009273/734721‑735019

 cgcgcgATCACACCAATGTTTTGATGCACGTTCAGGGTTATTTTCGCCCGCATATTGATTCCACAGAACGCCAGCAGCTGGCTGCGCTTATCGACAGTTATCGCCGTGGCGAGCAACCACTTCTTGCGCCGCTGATGCGTATCAAACACt                                                                                                                                                      <  1:3695678/150‑1 (MQ=255)

 cgcgcgATCACACCAATGTTTTGATGCACGTTCAGGGTTATTTTCGCCCGCATATTGATTCCACAGAACGCCAGCAGCTGGCTGCGCTTATCGACAGTTATCGCCGTGGCGAGCAACCACTTCTTGCGCCGCTGATGCGTATCAAACACt                                                                                                                                                      <  1:4644989/150‑1 (MQ=255)

 cgcgcgATCACACCAATGTTTTGATGCACGTTCAGGGTTATTTTCGCCCGCATATTGATTCCACAGAACGCCAGCAGCTGGCTGCGCTTATCGACAGTTATCGCCGTGGCGAGCAACCACTTCTTGCGCCGCTGATGCGTATCAAACACt                                                                                                                                                      <  1:5140504/150‑1 (MQ=255)

    gcgATCACACCAATGTTTTGATGCACGTTCAGGGTTATTTTCGCCCGCATATTGATTCCACAGAACGCCAGCAGCTGGCTGCGCTTATCGACAGTTATCGCCGTGGCGAGCAACCACTTCTTGCGCCGCTGATGCGTATCAAACACtata                                                                                                                                                   <  2:334696/150‑1 (MQ=255)

        tCACACCAATGTTTTGATGCACGTTCAGGGTTATTTTCGCCCGCATATTGATTCCACAGAACGCCAGCAGCTGGCTGCGCTTATCGACAGTTATCGCCGTGGCGAGCAACCACTTCTTGCGCCGCTGATGCGTATCAAACACTATATGgc                                                                                                                                               >  2:4490979/1‑150 (MQ=255)

           cacCAATGTTTTGATGCACGTTCAGGGTTATTTTCGCCCGCATATTGATTCCACAGAACGCCAGCAGCTGGCTGCGCTTATCGACAGTTATCGCCGTGGCGAGCAACCACTTCTTGCGCCGCTGATGCGTATCAAACACTATATGGCGCt                                                                                                                                            >  2:4550902/1‑150 (MQ=255)

            acCAATGTTTTGATGCACGTTCAGGGTTATTTTCGCCCGCATATTGATTCCACAGAACGCCAGCAGCTGGCTGCGCTTATCGACAGTTATCGCCGTGGCGAGCAACCACTTCTTGCGCCGCTGATGCGTATCAAACACTATATGGCGCtt                                                                                                                                           <  1:3513625/150‑1 (MQ=255)

             ccAATGTTTTGATGCACGTTCAGGGTTATTTTCGCCCGCATATTGATTCCACAGAACGCCAGCAGCTGGCTGCGCTTATCGACAGTTATCGCCGTGGCGAGCAACCACTTCTTGCGCCGCTGATGCGTATCAAACACTATATGGCGCttt                                                                                                                                          >  2:1911936/1‑150 (MQ=255)

               aaTGTTTTGATGCACGTTCAGGGTTATTTTCGCCCGCATATTGATTCCACAGAACGCCAGCAGCTGGCTGCGCTTATCGACAGTTATCGCCGTGGCGAGCAACCACTTCTTGCGCCGCTGATGCGTATCAAACACTATATGGCGCTTTAt                                                                                                                                        >  1:1511577/1‑150 (MQ=255)

                aTGTTTTGATGCACGTTCAGGGTTATTTTCGCCCGCATATTGATTCCACAGAACGCCAGCAGCTGGCTGCGCTTATCGACAGTTATCGCCGTGGCGAGCAACCACTTCTTGCGCCGCTGATGCGTATCAAACACTATATGGCGCTTTATc                                                                                                                                       <  1:448395/150‑1 (MQ=255)

                  gTTTTGATGCACGTTCAGGGTTATTTTCGCCCGCATATTGATTCCACAGAACGCCAGCAGCTGGCTGCGCTTATCGACAGTTATCGCCGTGGCGAGCAACCACTTCTTGCGCCGCTGATGCGTATCAAACACTATATGGCGCTTTATCCt                                                                                                                                     >  1:899226/1‑150 (MQ=255)

                    tttGATGCACGTTCAGGGTTATTTTCGCCCGCATATTGATTCCACAGAACGCCAGCAGCTGGCTGCGCTTATCGACAGTTATCGCCGTGGCGAGCAACCACTTCTTGCGCCGCTGATGCGTATCAAACACTATATGGCGCTTTATCCTGa                                                                                                                                   >  2:635263/1‑150 (MQ=255)

                    tttGATGCACGTTCAGGGTTATTTTCGCCCGCATATTGATTCCACAGAACGCCAGCAGCTGGCTGCGCTTATCGACAGTTATCGCCGTGGCGAGCAACCACTTCTTGCGCCGCTGATGCGTATCAAACACTATATGGCGCTTTATCCTGa                                                                                                                                   >  1:3027254/1‑150 (MQ=255)

                        aTGCACGTTCAGGGTTATTTTCGCCCGCATATTGATTCCACAGAACGCCAGCAGCTGGCTGCGCTTATCGACAGTTATCGCCGTGGCGAGCAACCACTTCTTGCGCCGCTGATGCGTATCAAACAATATATGGCGCTTTATCCTGACGcc                                                                                                                               >  1:113085/1‑150 (MQ=255)

                         tGCACGTTCAGGGTTATTTTCGCCCGCATATTGATTCCACAGAACGCCAGCAGCTGGCTGCGCTTATCGACAGTTATCGCCGTGGCGAGCAACCACTTCTTGCGCCGCTGATGCGTATCAAACACTATATGGCGCTTTATCCTGACGCCt                                                                                                                              >  1:2163262/1‑150 (MQ=255)

                         tGCACGTTCAGGGTTATTTTCGCCCGCATATTGATTCCACAGAACGCCAGCAGCTGGCTGCGCTTATCGACAGTTATCGCCGTGGCGAGCAACCACTTCTTGCGCCGCTGATGCGTATCAAACACTATATGGCGCTTTATCCTGACGCCt                                                                                                                              >  2:3465895/1‑150 (MQ=255)

                          gCACGTTCAGGGTTATTTTCGCCCGCATATTGATTCCACAGAACGCCAGCAGCTGGCTGCGCTTATCGACAGTTATCGCCGTGGCGAGCAACCACTTCTTGCGCCGCTGATGCGTATCAAACACTATATGGCGCTTTATCCTGACGCCTg                                                                                                                             <  2:2634109/150‑1 (MQ=255)

                           cACGTTCAGGGTTATTTTCGCCCGCATATTGATTCCACAGAACGCCAGCAGCTGGCTGCGCTTATCGACAGTTCTCGCCGTGGCGAGCAACCACTTCTTGCGCCGCTGATGCGTATCAAACACTATATGGCGCTTTATCCTGACGCCTgg                                                                                                                            >  2:614673/1‑150 (MQ=255)

                              gTTCAGGGTTATTTTCGCCCGCATATTGATTCCACAGAACGCCAGCAGCTGGCTGCGCTTATCGACAGTTATCGCCGTGGCGAGCAACCACTTCTTGCGCCGCTGATGCGTATCAAACACTATATGGCGCTTTATCCTGACGCCTGGCtt                                                                                                                         <  1:184401/150‑1 (MQ=255)

                               ttCAGGGTTATTTTCGCCCGCATATTGATTCCACAGAACGCCAGCAGCTGGCTGCGCTTATCGACAGTTATCGCCGTGGCGAGCAACCACTTCTTGCGCCGCTGATGCGTATCAAACACTATATGGCGCTTTATCCTGACGCCTGGCttt                                                                                                                        <  1:3155664/150‑1 (MQ=255)

                                 cAGGGTTATTTTCGCCCGCATATTGATTCCACAGAACGCCAGCAGCTGGCTGCGCTTATCGACAGTTATCGCCGTGGCGAGCAACCACTTCTTGCGCCGCTGATGCGTATCAAACACTATATGGCGCTTTATCCTGACGCCTGGCTTTCa                                                                                                                      >  1:1768670/1‑150 (MQ=255)

                                  aGGGTTATTTTCGCCCGCATATTGATTCCACAGAACCCCAGCAGCTGGCTGCGCTTATCGACAGTTATCGCCGTGGCGAGCAACCACTCCTTGCGCCGCTGATGCGTATCAAACACTATATGGCGCTTTATCCTGACGCCTGGCTTTCAg                                                                                                                     >  1:4143557/1‑150 (MQ=255)

                                      ttATTTTCGCCCGCATTTTGATTCCACAGAACGCCAGCAGCTGGCTGCGCTTATCGACAGTTATCGCCGTGGCGAGCAACCACTTCTTGCTCCGCTGATGCGTATCAAACACTATATGGCGCTTTATCCTGACGCCTGGCTTTCAGGGCa                                                                                                                 <  1:96726/150‑1 (MQ=255)

                                       tATTTTCGCCCGCATATTGATTCCACAGAACGCCAGCAGCTGGCTGCGCTTATCGACAGTTATCGCCGTGGCGAGCAACCACTTCTTGCGCCGCTGATGCGTATCAAACACTATATGGCGCTTTATCCTGACGCCTGGCTTTCAGGGCAg                                                                                                                >  2:921696/1‑150 (MQ=255)

                                        aTTTTCGCCCGCATATTGATTCCACAGAACGCCAGCAGCTGGCTGCGCTTATCGACAGTTATCGCCGTGGCGAGCAACCACTTCTTGCGCCGCTGATGCGTATCAAACAATATATGGCGCTTTATCCTGACGCCTGGCTTTCAGGGCAGc                                                                                                               <  1:4831214/150‑1 (MQ=255)

                                           ttCGCCCGCATATTGATTCCACAGAACGCCAGCAGCTGGCTGCGCTTATCGACAGTTATCGCCGTGGCGAGCAACCACTTCTTGCGCCGCTGATGCGTATCAAACACTATATGGCGCTTTATCCTGACGCCTGGCTTTCAGGGCAGCGtt                                                                                                            <  1:3620039/150‑1 (MQ=255)

                                              gCCCGCATATTGATTCCACAGAACGCCAGCAGCTGGCTGCGCTTATCGACAGTTATCGCCGTGGCGAGCAACCACTTCTTGCGCCGCTGATGCGTATCAAACACTATATGGCGCTTTATCCTGACGCCTGGCTTTCAGGGCAGCGTTAtt                                                                                                         <  1:908814/150‑1 (MQ=255)

                                               cccGCATATTGATTCCACAGAACGCCAGCAGCTGGCTGCGCTTATCGACAGTTATCGCCGTGGCGAGCAACCACTTCTTGCGCCGCTGATGCGTATCAAACACTATATGGCGCTTTATCCTGACGCCTGGCTTTCAGGGCAGCGTTAttt                                                                                                        >  1:4419132/1‑150 (MQ=255)

                                                ccGCATCTTGAGTCCCCAGACCGCCAGCAGCTGGCTGCGCTGATCGACAGTTATCGCCGTGGCGAGCAACCACGTCGTGCGCCGCTGCTGCGTATCAAACACTATCTGGCGCTTTCTCCTGACGCCTGGCTTTCAGGGCAGCGTTATTTc                                                                                                       <  2:2595487/150‑1 (MQ=255)

                                                ccGCATATTGATTCCACAGAACGCCAGCAGCTGGCTGCGCTTATCGACAGTTATCGCCGTGGCGAGCAACCACTTCTTGCGCCGCTGATGCGTATCAAACACTATATGGCGCTTTATCCTGACGCCTGGCTTTCAGGGCAGCGTTATTTc                                                                                                       <  2:1857910/150‑1 (MQ=255)

                                                ccGCATATTGATTCCACAGAACGCCAGCAGCTGGCTGCGCTTATCGACAGTTATCGCCGTGGCGAGCAACCACTTCTTGCGCCGCTGATGCGTATCAAACACTATATGGCGCTTTATCCTGACGCCTGGCTTTCAGGGCAGCGTTATTTc                                                                                                       <  2:369685/150‑1 (MQ=255)

                                                 cGCATATTGATTCCACAGAACGCCAGCAGCTGGCTGCGCTTATCGACAGTTATCGCCGTGGCGAGCAACCACTTCTTGCGCCGCTGATGCGTATCAAACACTATATGGCGCTTTATCCTGACGCCTGGCTTTCAGGGCAGCGTTATTTCg                                                                                                      <  2:4515136/150‑1 (MQ=255)

                                                  gCATATTGATTCCACAGAACGCCAGCAGCTGGCTGCGCTTATCGACAGTTATCGCCGTGGCGAGCAACCACTTCTTGCGCCGCTGATGCGTATCAAACACTATATGGCGCTTTATCCTGACGCCTGGCTTTCAGGGCAGCGTTATTTCGa                                                                                                     <  1:2779752/150‑1 (MQ=255)

                                                    atatTGATTCCACAGAACGCCAGCAGCTGGCTGCGCTTATCGACAGTTATCGCCGTGGCGAGCAACCACTTCTTGCGCCGCTGATGCGTATCAAACACTATATGGCGCTTTATCCTGACGCCTGGCTTTCAGGGCAGCGTTATTTCGAAc                                                                                                   <  1:785337/150‑1 (MQ=255)

                                                       ttGATTCCACAGAACGCCAGCAGCTGGCTGCGCTTATCGACAGTTATCGCCGTGGCGAGCAACCACTTCTTGCGCCGCTGATGCGTATCAAACACTATATGGCGCTTTATCCTGACGCCTGGCTTTCAGGGCAGCGTTATTTCGAACttt                                                                                                <  2:4035021/150‑1 (MQ=255)

                                                           ttCCACAGAACGCCAGCAGCTGGCTGCGCTTATCGACAGTTATCGCCGTGGCGAGCAACCACTTCTTGCGCCGCTGATGCGTATCAAACACTATATGGCGCTTTATCCTGACGCCTGGCTTTCAGGGCAGCGTTATTTCGAACTTTGGcc                                                                                            <  1:3772032/150‑1 (MQ=255)

                                                           ttCCACAGAACGCCAGCAGCTGGCTGCGCTTATCGACAGTTATCGCCGTGGCGAGCAACCACTTCTTGCGCCGCTGATGCGTATCAAACACTATATGGCGCTTTATCCTGACGCCTGGCTTTCAGGGCAGCGTTATTTCGAACTTTGGcc                                                                                            <  2:3496777/150‑1 (MQ=255)

                                                               acaGAACGCCAGCAGCTGGCTGCGCTTATCGACAGTTATCGCCGTGGCGAGCAACCACTTCTTGCGCCGCTGATGCGTATCAAACACTATATGGCGCTTTATCCTGACGCCTGGCTTTCAGGGCAGCGTTATTTCGAACTTTGGCCGCgt                                                                                        >  2:2968541/1‑150 (MQ=255)

                                                                caGAACGCCAGCAGCTGGCTGCGCTTATCGACAGTTATCGCCGTGGCGAGCAACCACTTCTTGCGCCGCTGATGCGTATCAAACACTATATGGCGCTTTATCCTGACGCCTGGCTTTCAGGGCAGCGTTATTTCGAACTTTGGCCGCgtg                                                                                       >  2:2840317/1‑150 (MQ=255)

                                                                caGAACGCCAGCAGCTGGCTGCGCTTATCGACAGTTATCGCCGTGGCGAGCAACCACTTCTTGCGCCGCTGATGCGTATCAAACACTATATGGCGCTTTATCCTGACGCCTGGCTTTCAGGGCAGCGTTATTTCGAACTTTGGCCGCgtg                                                                                       >  2:37795/1‑150 (MQ=255)

                                                                 aGAACGCCAGCAGCTGGCTGCGCTTATCGACAGTTATCGCCGTGGCGAGCAACCACTTCTTGCGCCGCTGATGCGTATCAAACACTATATGGCGCTTTATCCTGACGCCTGGCTTTCAGGGCAGCGTTATTTCGAACTTTGGCCGCgtgt                                                                                      >  2:3912963/1‑150 (MQ=255)

                                                                 aGAACGCCAGCAGCTGGCTGCGCTTATCGACAGTTATCGCCGTGGCGAGCAACCACTTCTTGCGCCGCTGATGCGTATCAAACACTATATGGCGCTTTATCCTGACGCCTGGCTTTCAGGGCAGCGTTATTTCGAACTTTGGCCGCgtgt                                                                                      <  1:1894723/150‑1 (MQ=255)

                                                                 aGAACGCCAGCAGCTGGCTGCGCTTATCGACAGTTATCGCCGTGGCGAGCAACCACTTCTTGCGCCGCTGATGCGTATCAAACACTATATGGCGCTTTATCCTGACGCCTGGCTTTCAGGGCAGCGTTATTTCGAACTTTGGCCGCgtgt                                                                                      <  2:2019933/150‑1 (MQ=255)

                                                                  gAACGCCAGCAGCTGGCTGCGCTTATCGACAGTTATCGCCGTGGCGAGCAACCACTTCTTGCGCCGCTGATGCGTATCAAACACTATATGGCGCTTTATCCTGACGCCTGGCTTTCAGGGCAGCGTTATTTCGAACTTTGGCCGCGtgtg                                                                                     <  2:2750091/150‑1 (MQ=255)

                                                                    aCGCCAGCAGCTGGCTGCGCTTATCGACAGTTATCGCCGTGGCGAGCAACCACTTCTTGCGCCGCTGATGCGTATCAAACACTATATGGCGCTTTATCCTGACGCCTGGCTTTCAGGGCAGCGTTATTTCGAACTTTGGCCGCGTGTGAt                                                                                   <  2:4920097/150‑1 (MQ=255)

                                                                      gCCAGCAGCTGGCTGCGCTTATCGACAGTTATCGCCGTGGCGAGCAACCACTTCTTGCGCCGCTGATGCGTATCAAACACTATATGGCGCTTTATCCTGACGCCTGGCTTTCAGGGCAGCGTTATTTCGAACTTTGGCCGCGTGTGATTa                                                                                 >  1:2962539/1‑150 (MQ=255)

                                                                         agcagcTGGCTGCGCTTATCGACAGTTATCGCCGTGGCGAGCAACCACTTCTTGCGCCGCTGATGCGTATCAAACACTATATGGCGCTTTATCCTGACGCCTGGCTTTCAGGGCAGCGTTATTTCGAACTTTGGCCGCGTGTGATTAACt                                                                              >  2:4154560/1‑150 (MQ=255)

                                                                          gcagcTGGCTGCGCTTATCGACAGTTATCGCCGTGGCGAGCAACCACTTCTTGCGCCGCTGATGCGTATCAAACACTATATGGCGCTTTATCCTGACGCCTGGCTTTCAGGGCAGCGTTATTTCGAACTTTGGCCGCGTGTGATTAACtt                                                                             <  2:199245/150‑1 (MQ=255)

                                                                            agcTGGCTGCGCTTATCGACAGTTATCGCCGTGGCGAGCAACCACTTCTTGCGCCGCTGATGCGTATCAAACACTATATGGCGCTTTATCCTGACGCCTGGCTTTCAGGGCAGCGTTATTTCGAACTTTGGCCGCGTGTGATTAACTTgc                                                                           >  1:4922219/1‑150 (MQ=255)

                                                                              ctggctgCGCTTATCGACAGTTATCGCCGTGGCGAGCAACCACTTCTTGCGCCGCTGATGCGTATCAAACACTATATGGCGCTTTATCCTGACGCCTGGCTTTCAGGGCAGCGTTATTTCGAACTTTGGCCGCGTGTGATTAACTTgcgc                                                                         >  1:1971103/1‑150 (MQ=255)

                                                                               tggctgCGCTTATCGACAGTTATCGCCGTGGCGAGCAACCACTTCTTGCGCCGCTGATGCGTATCAAACACTATATGGCGCTTTATCCTGACGCCTGGCTTTCAGGGCAGCGTTATTTCGAACTTTGGCCGCGTGTGATTAACTTGCGcc                                                                        <  1:4297269/150‑1 (MQ=255)

                                                                                 gctgCGCTTATCGACAGTTATCGCCGTGGCGAGCAACCACTTCTTGCGCCGCTGATGCGTATCAAACACTATATGGCGCTTTATCCTGACGCCTGGCTTTCAGGGCAGCGTTATTTCGAACTTTGGCCGCGTGTGATTAACTTGCGCCAt                                                                      >  2:428592/1‑150 (MQ=255)

                                                                                  ctgCGCTTATCGACAGTTATCGCCGTGGCGAGCAACCACTTCTTGCGCCGCTGATGCGTATCAAACACTATATGGCGCTTTATCCTGACGCCTGGCTTTCAGGGCAGCGTTATTTCGAACTTTGGCCGCGTGTGATTAACTTGCGCCAtt                                                                     >  1:3957084/1‑150 (MQ=255)

                                                                                  ctgCGCTTATCGACAGTTATCGCCGTGGCGAGCAACCACTTCTTGCGCCGCTGATGCGTATCAAACACTATATGGCGCTTTATCCTGACGCCTGGCTTTCAGGGCAGCGTTATTTCGAACTTTGGCCGCGTGTGATTAACTTGCGCCAtt                                                                     >  1:3361540/1‑150 (MQ=255)

                                                                                  ctgCGCTTATCGACAGTTATCGCCGTGGCGAGCAACCACTTCTTGCGCCGCTGATGCGTATCAAACAATATATGGCGCTTTATCCTGACGCCTGGCTTTCAGGGCAGCGTTATTTCGAACTTTGGCCGCGTGTGATTAACTTGCGCCAtt                                                                     <  1:2834752/150‑1 (MQ=255)

                                                                                   tgCGCTTATCGACAGTTATCGCCGTGGCGAGCAACCACTTCTTGCGCCGCTGATGCGTATCAAACACTATATGGCGCTTTATCCTGACGCCTGGCTTTCAGGGCAGCGTTATTTCGAACTTTGGCCGCGTGTGATTAACTTGCGCCATTc                                                                    <  2:771105/150‑1 (MQ=255)

                                                                                   tgCGCTTATCGACAGTTATCGCCGTGGCGAGCAACCACTTCTTGCGCCGCTGATGCGTATCAAACACTATATGGCGCTTTATCCTGACGCCTGGCTTTCAGGGCAGCGTTATTTCGAACTTTGGCCGCGTGTGATTAACTTGCGCCATTc                                                                    >  2:2289196/1‑150 (MQ=255)

                                                                                    gcgcTTATCGACAGTTATCGCCGTGGCGAGCAACCACTTCTTGCGCCGCTGATGCGTATCAAACACTATATGGCGCTTTATCCTGACGCCTGGCTTTCAGGGCAGCGTTATTTCGAACTTTGGCCGCGTGTGATTAACTTGCGCCATTCa                                                                   <  2:3843023/150‑1 (MQ=255)

                                                                                    gcgcTTATCGACAGTTATCGCCGTGGCGAGCAACCACTTCTTGCGCCGCTGATGCGTATCAAACACTATATGGCGCTTTATCCTGACGCCTGGCTTTCAGGGCAGCGTTATTTCGAACTTTGGCCGCGTGTGATTAACTTGCGCCATTCa                                                                   <  1:4124841/150‑1 (MQ=255)

                                                                                          aTCGACAGTTATCGCCGTGGCGAGCAACCACTTCTTGCGCCGCTGATGCGTATCAAACACTATATGGCGCTTTATCCTGACGCCTGGCTTTCAGGGCAGCGTTATTTCGAACTTTGGCCGCGTGTGATTAACTTGCGCCATTCAGGAGtt                                                             <  2:175331/150‑1 (MQ=255)

                                                                                           tCGACAGTTATCGCCGTGGCGAGCAACCACTTCTTGCGCCGCTGATGCGTATCAAACACTATATGGCGCTTTATCCTGACGCCTGGCTTTCAGGGCAGCGTTATTTCGAACTTTGGCCGCGTGTGATTAACTTGCGCCATTCAGGAGttt                                                            <  1:5004527/150‑1 (MQ=255)

                                                                                               cAGTTATCGCCGTGGCGAGCAACCACTTCTTGCGCCGCTGATGCGTATCAAACACTATATGGCGCTTTATCCTGACGCCTGGCTTTCAGGGCAGCGTTATTTCGAACTTTGGCCGCGTGTGATTAACTTGCGCCATTCAGGAGTTTTATg                                                        <  2:4416435/150‑1 (MQ=255)

                                                                                               cAGTTATCGCCGTGGCGAGCAACCACTTCTTGCGCCGCTGATGCGTATCAAACACTATATGGCGCTTTATCCTGACGCCTGGCTTTCAGGGCAGCGTTATTTCGAACTTTGGCCGCGTGTGATTAACTTGCGCCATTCAGGAGTTTTATg                                                        <  1:2624235/150‑1 (MQ=255)

                                                                                                 gTTATCGCCGTGGCGAGCAACCACTTCTTGCGCCGCTGATGCGTATCAAACACTATATGGCGCTTTATCCTGACGCCTGGCTTTCAGGGCAGCGTTATTTCGAACTTTGGCCGCGTGTGATTAACTTGCGCCATTCAGGAGTTTTATGAc                                                      >  2:4778856/1‑150 (MQ=255)

                                                                                                   tATCGCCGTGGCGAGCAACCACTTCTTGCGCCGCTGATGCGTATCAAACACTATATGGCGCTTTATCCTGACGCCTGGCTTTCAGGGCAGCGTTATTTCGAACTTTGGCCGCGTGTGATTAACTTGCGCCATTCAGGAGTTTTATGACTa                                                    >  1:3373127/1‑150 (MQ=255)

                                                                                                    aTCGCCGTGGCGAGCAACCACTTCTTGCGCCGCTGATGCGTATCAAACACTATATGGCGCTTTATCCTGACGCCTGGCTTTCAGGGCAGCGTTATTTCGAACTTTGGCCGCGTGTGATTAACTTGCGCCATTCAGGAGTTTTATGACTAc                                                   <  1:4367502/150‑1 (MQ=255)

                                                                                                    aTCGCCGTGGCGAGCAACCACTTCTTGCGCCGCTGATGCGTATCAAACACTATATGGCGCTTTATCCTGACGCCTGGCTTTCAGGGCAGCGTTATTTCGAACTTTGGCCGCGTGTGATTAACTTGCGCCATTCAGGAGTTTTATGACTAc                                                   >  1:3652609/1‑150 (MQ=255)

                                                                                                     tCGCCGTGGCGAGCAACCACTTCTTGCGCCGCTGATGCGTATCAAACACTATATGGCGCTTTATCCTGACGCCTGGCTTTCAGGGCAGCGTTATTTCGAACTTTGGCCGCGTGTGATTAACTTGCGCCATTCAGGAGTTTTATGACTAcc                                                  <  2:4767387/150‑1 (MQ=255)

                                                                                                      cGCCGTGGCGAGCAACCACTTCTTGCGCCGCTGATGCGTATCAAACACTATATGGCGCTTTATCCTGACGCCTGGCTTTCAGGGCAGCGTTATTTCGAACTTTGGCCGCGTGTGATTAACTTGCGCCATTCAGGAGTTTTATGACTAccc                                                 <  1:1376785/150‑1 (MQ=255)

                                                                                                          gTGGCGAGCAACCACTTCTTGCGCCGCTGATGCGTATCAAACAATATATGGCGCTTTATCCTGACGCCTGGCTTTCAGGGCAGCGTTATTTCGAACTTTGGCCGCGTGTGATTAACTTGCGCCATTCAGGAGTTTTATGACTACCCAtct                                             >  2:1303281/1‑150 (MQ=255)

                                                                                                              cGAGCAACCACTTCTTGCGCCGCTGATGCGTATCAAACACTATATGGCGCTTTATCCTGACGCCTGGCTTTCAGGGCAGCGTTATTTCGAACTTTGGCCGCGTGTGATTAACTTGCGCCATTCAGGAGTTTTATGACTACCCAtctggtc                                         <  2:86434/150‑1 (MQ=255)

                                                                                                                aGCAACCACTTCTTGCGCCGCTGATGCGTATCAAACACTATATGGCGCTTTATCCTGACGCCTGGCTTTCAGGGCAGCGTTATTTCGAACTTTGGCCGCGTGTGATTAACTTGCGCCATTCAGGAGTTTTATGACTACCCAtctggtctg                                       <  2:537345/150‑1 (MQ=255)

                                                                                                                 gCAACCACTTCTTGCGCCGCTGATGCGTATCAAACACTATATGGCGCTTTATCCTGACGCCTGGCTTTCAGGGCAGCGTTATTTCGAACTTTGGCCGCGTGTGATTAACTTGCGCCATTCAGGAGTTTTATGACTACCCAtctggtctgg                                      >  2:392129/1‑150 (MQ=255)

                                                                                                                   aaCCACTTCTTGCGCCGCTGATGCGTATCAAACACTATATGGCGCTTTATCCTGACGCCTGGCTTTCAGGGCAGCGTTATTTCGAACTTTGGCCGCGTGTGATTAACTTGCGCCATTCAGGAGTTTTATGACTACCCATCTGGTCTGGtt                                    >  2:928599/1‑150 (MQ=255)

                                                                                                                   aaCCACTTCTTGCGCCGCTGATGCGTATCAAACAATATATGGCGCTTTATCCTGACGCCTGGCTTTCAGGGCAGCGTTATTTCGAACTTTGGCCGCGTGTGATTAACTTGCGCCATTCAGGAGTTTTATGACTACCCATCTGGTCTGGtt                                    >  2:1087460/1‑150 (MQ=255)

                                                                                                                   aaCCACTTCTTGCGCCGCTGATGCGTATCAAACAATATATGGCGCTTTATCCTGACGCCTGGCTTTCAGGGCAGCGTTATTTCGAACTTTGGCCGCGTGTGATTAACTTGCGCCATTCAGGAGTTTTATGACTACCCATCTGGTCTGGtt                                    >  2:888908/1‑150 (MQ=255)

                                                                                                                    aCCACTTCTTGCGCCGCTGATGCGTATCAAACACTATATGGCGCTTTATCCTGACGCCTGGCTTTCAGGGCAGCGTTATTTCGAACTTTGGCCGCGTGTGATTAACTTGCGCCATTCAGGAGTTTTATGACTACCCATCTGGTCTGGttt                                   <  1:4691620/150‑1 (MQ=255)

                                                                                                                     ccACTTCTTGCGCCGCTGATGCGTATCAAACACTATATGGCGCTTTATCCTGACGCCTGGCTTTCAGGGCAGCGTTATTTCGAACTTTGGCCGCGTGTGATTAACTTGCGCCATTCAGGAGTTTTATGACTACCCATCTGGTCTGGTTTc                                  <  1:100454/150‑1 (MQ=255)

                                                                                                                     ccACTTCTTGCGCCGCTGATGCGTATCAAACAATATATGGCGCTTTATCCTGACGCCTGGCTTTCAGGGCAGCGTTATTTCGAACTTTGGCCGCGTGTGATTAACTTGCGCCATTCAGGAGTTTTATGACTACCCATCTGGTCTGGTTTc                                  <  1:1631708/150‑1 (MQ=255)

                                                                                                                      cACTTCTTGCGCCGCTGATGCGTATCAAACACTATATGGCGCTTTATCCTGACGCCTGGCTTTCAGGGCAGCGTTATTTCGAACTTTGGCCGCGTGTGATTAACTTGCGCCATTCAGGAGTTTTATGACTACCCATCTGGTCTGGTTTCg                                 >  1:2466839/1‑150 (MQ=255)

                                                                                                                      cACTTCTTGCGCCGCTGATGCGTATCAAACACTATATGGCGCTTTATCCTGACGCCTGGCTTTCAGGGCAGCGTTATTTCGAACTTTGGCCGCGTGTGATTAACTTGCGCCATTCAGGAGTTTTATGACTACCCATCTGGTCTGGTTTCg                                 >  2:1348334/1‑150 (MQ=255)

                                                                                                                        cttcttGCGCCGCTGATGCGTATCAAACACTATATGGCGCTTTATCCTGACGCCTGGCTTTCAGGGCAGCGTTATTTCGAACTTTGGCCGCGTGTGATTAACTTGCGCCATTCAGGAGTTTTATGACTACCCATCTGGTCTGGTTTCGcc                               <  1:4752146/150‑1 (MQ=255)

                                                                                                                        cttcttGCGCCGCTGATGCGTATCAAACACTATATGGCGCTTTATCCTGACGACTGGCTTTCAGGGCAGCGTTATTTCGAACTTTGGCCGCGTGTGATTAACTTGCGCCATTCAGGAGTTTTATGACTACCCATCTGGTCTGGTTTCGcc                               <  2:2781222/150‑1 (MQ=255)

                                                                                                                         ttcttGCGCCGCTGATGCGTATCAAACACTATATGGCGCTTTATCCTGACGCCTGGCTTTCAGGGCAGCGTTATTTCGAACTTTGGCCGCGTGTGATTAACTTGCGCCATTCAGGAGTTTTATGACTACCCATCTGGTCTGGTTTCGCCa                              <  2:3619673/150‑1 (MQ=255)

                                                                                                                         ttcttGCGCCGCTGATGCGTATCAAACACTATATGGCGCTTTATCCTGACGCCTGGCTTTCAGGGCAGCGTTATTTCGAACTTTGGCCGCGTGTGATTAACTTGCGCCATTCAGGAGTTTTATGACTACCCATCTGGTCTGGTTTCGCCa                              >  2:3062510/1‑150 (MQ=255)

                                                                                                                          tcttGCGCCGCTGATGCGTATCAAACACTATATGGCGCTTTATCCTGACGCCTGGCTTTCAGGGCAGCGTTATTTCGAACTTTGGCCGCGTGTGATTAACTTGCGCCATTCAGGAGTTTTATGACTACCCATCTGGTCTGGTTTCGCCAg                             >  1:1247647/1‑150 (MQ=255)

                                                                                                                               cgccgcTGATGCGTATCAAACACTATATGGCGCTTTATCCTGACGCCTGGCTTTCAGGGCAGCGTTATTTCGAACTTTGGCCGCGTGTGATTAACTTGCGCCATTCAGGAGTTTTATGACTACCCATCTGGTCTGGTTTCGCCAGGAttt                        <  1:577055/150‑1 (MQ=255)

                                                                                                                                   gcTGATGCGTATCAAACACTATATGGCGCTTTATCCTGACGCCTGGCTTTCAGGGCAGCGTTATTTCGAACTTTGGCCGCGTGTGATTAACTTGCGCCATTCAGGAGTTTTATGACTACCCATCTGGTCTGGTTTCGCCAGGATTTACGt                    <  2:3435226/150‑1 (MQ=255)

                                                                                                                                     tGATGCGTATCAAACACTATATGGCGCTTTATCCTGACGCCTGGCTTTCAGGGCAGCGTTATTTCGAACTTTGGCCGCGTGTGATTAACTTGCGCCATTCAGGAGTTTTATGACTACCCATCTGGTCTGGTTTCGCCAGGATTTACGTCt                  <  1:1227618/150‑1 (MQ=255)

                                                                                                                                          cGTATCAAACACTATATGGCGCTTTATCCTGACGCCTGGCTTTCAGGGCAGCGTTATTTCGAACTTTGGCCGCGTGTGATTAACTTGCGCCATTCAGGAGTTTTATGACTACCCATCTGGTCTGTTTTCGACAGGATTTACGTCTGCACg             <  1:2332832/150‑1 (MQ=255)

                                                                                                                                          cGTATCAAACACTATATGGCGCTTTATCCTGACGCCTGGCTTTCAGGGCAGCGTTATTTCGAACTTTGGCCGCGTGTGATTAACTTGCGCCATTCAGGAGTTTTATGACTACCCATCTGGTCTGGTTTCGCCAGGATTTACGTCTGCACg             <  1:5075346/150‑1 (MQ=255)

                                                                                                                                          cGTATCAAACACTATATGGCGCTTTATCCTGACGCCTGGCTTTCAGGGCAGCGTTATTTCGAACTTTGGCCGCGTGTGATTAACTTGCGCCATTCAGGAGTTTTATGACTACCCATCTGGTCTGGTTTCGCCAGGATTTACGTCTGCACg             <  1:404999/150‑1 (MQ=255)

                                                                                                                                           gTATCAAACACTATATGGCGCTTTATCCTGACGCCTGGCTTTCAGGGCAGCGTTATTTCGAACTTTGGCCGCGTGTGATTAACTTGCGCCATTCAGGAGTTTTATGACTACCCATCTGGTCTGGTTTCGCCAGGATTTACGTCTGCACGa            >  2:4911327/1‑150 (MQ=255)

                                                                                                                                              tCAAACACTATATGGCGCTTTATCCTGACGCCTGGCTTTCAGGGCAGCGTTATTTCGAACTTTGGCCGCGTGTGATTAACTTGCGCCATTCAGGAGTTTTATGACTACCCATCTGGTCTGGTTTCGCCAGGATTTACGTCTGCACGATaa         >  1:4316493/1‑150 (MQ=255)

                                                                                                                                                aaaCACTATATGGCGCTTTATCCTGACGCCTGGCTTTCAGGGCAGCGTTATTTCGAACTTTGGCCGCGTGTGATTAACTTGCGCCATTCAGGAGTTTTATGACTACCCATCTGGTCTGGTTTCGCCAGGATTTACGTCTGCACGATAAtc       >  1:4451862/1‑150 (MQ=255)

                                                                                                                                                 aaCAATATATGGCGCTTTATCCTGACGCCTGGCTTTCAGGGCAGCGTTATTTCGAACTTTGGCCGCGTGTGATTAACTTGCGCCATTCAGGAGTTTTATGACTACCCATCTGGTCTGGTTTCGCCAGGATTTACGTCTGCACGATAAtct      >  2:1964175/1‑150 (MQ=255)

                                                                                                                                                  acacTATATGGCGCTTTATCCTGACGCCTGGCTTTCAGGGCAGCGTTATTTCGAACTTTGGCCGCGTGTGATTAACTTGCGCCATTCAGGAGTTTTATGACTACCCATCTGGTCTGGTTTCGCCAGGATTTACGTCTGCACGATAAtctc     >  1:2314156/1‑150 (MQ=255)

                                                                                                                                                  acacTATATGGCGCTTTATCCTGACGCCTGGCTTTCAGGGCAGCGTTATTTCGAACTTTGGCCGCGTGTGATTAACTTGCGCCATTCAGGAGTTTTATGACTACCCATCTGGTCTGGTTTCGCCAGGATTTACGTCTGCACGATAAtctc     >  2:1616406/1‑150 (MQ=255)

                                                                                                                                                  acacTATATGGCGCTTTATCCTGACGCCTGGCTTTCAGGGCAGCGTTATTTCGAACTTTGGCCGCGTGTGATTAACTTGCGCCAATCAGGAGTTTTATGACTACCCATCTGGTCTGGTTTCGCCAGGATTTAAGTCTGCACTATAAtctc     >  2:509606/1‑150 (MQ=255)

                                                                                                                                                     cTATATGGCGCTTTATCCTGACGCCTGGCTTTCAGGGCAGCGTTATTTCGAACTTTGGCCGCGTGTGATTAACTTGCGCCATTCAGGAGTTTTATGACTACCCATCTGGTCTGGTTTCGCCAGGATTTACGTCTGCACGATAATCTCGCa  >  2:2891585/1‑150 (MQ=255)

CCGCGCGATCACACCAATGTTTTGATGCACGTTCAGGGTTATTTTCGCCCGCATATTGATTCCACAGAACGCCAGCAGCTGGCTGCGCTTATCGACAGTTATCGCCGTGGCGAGCAACCACTTCTTGCGCCGCTGATGCGTATCAAACACTATATGGCGCTTTATCCTGACGCCTGGCTTTCAGGGCAGCGTTATTTCGAACTTTGGCCGCGTGTGATTAACTTGCGCCATTCAGGAGTTTTATGACTACCCATCTGGTCTGGTTTCGCCAGGATTTACGTCTGCACGATAATCTCGCA  >  CP009273/734721‑735019

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`