Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 818,362 G→T 21.3% G278G (GGC→GGA)  clsB ← cardiolipin synthase 2

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273818,3620GT21.3% 753.8 / 157.8 282G278G (GGC→GGA) clsBcardiolipin synthase 2
Reads supporting (aligned to +/- strand):  ref base G (117/105);  new base T (30/30);  total (147/135)
Fisher's exact test for biased strand distribution p-value = 7.71e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.75e-01

Only 100 of 299 total aligned reads displayed.

AAATGACGATCGTGGATGATGACATTTGCTTCGAGATTCAGTGACAAACTGAGCGGATCGAGATTACTGGACCCTACTGTCGCCCAGTGATCGTCCATCAATGCCACTTTGCCGTGGAGCGGGCGGCGGCGGTACTC‑AAAAACCTGAACGCCGCCTTTAACCAGATAGTTATACAGCAAGCGCGCACCGACTCTGACAATCGGCATATCCGGTTCGCCCTGAATGATCAGTTTGATCCGCACCCCGCGCCGTGCCGCTTTACGCAAGGCGTGTAAAAATCGATAGCCGGGGAAGAAGTA  >  CP009273/818213‑818511
                                                                                                                                                      |                                                                                                                                                     
aaaTGACGATCGTGGATGATGACATTTGCTTCGAGATTCAGTGACAAACTGAGCGGATCGAGATTACTGGACCCTACTGTCGCCCAGTGATCGTCCATCAATGCCACTTTGCCGTGGAGCGGGCGGCGGCGGTACTC‑AAAAACCTGAAcg                                                                                                                                                       >  2:3969430/1‑150 (MQ=255)
 aaTGACGATCGTGGATGATGACATTTGCTTCGAGATTCAGTGACAAACTGAGCGGATCGAGATTACTGGACCCTACTGTCGCCCAGTGATCGTCCATCAATGCCACTTTGCCGTGGAGCGGGCGGCGGCGGTACTC‑AAAAACCTGAAcgc                                                                                                                                                      >  2:4969018/1‑150 (MQ=255)
  aTGACGATCGTGGATGATGACATTTGCTTCGAGATTCAGTGACAAACTGAGCGGATCGAGATTACTGGACCCTACTGTCGCCCAGTGATCGTCCATCAATGCCACTTTGCCGTGGAGCGGGCGGCGGCGGTACTC‑AAAAACCTGAActcc                                                                                                                                                     >  1:1813719/1‑150 (MQ=255)
  aTGACGATCGTGGATGATGACATTTGCTTCGAGATTCAGTGACAAACTGAGCGGATCGAGATTACTGGACCCTACTGTCGCCCAGTGATCGTCCATCAATGCCACTTTGCCGTGGAGCGGGCGGCGGCGGTACTC‑AAAAACCTGAAcgcc                                                                                                                                                     <  1:2913165/150‑1 (MQ=255)
  aTGACGATCGTGGATGATGACATTTGCTTCGAGATTCAGTGACAAACTGAGCGGATCGAGATTACTGGACCCTACTGTCGCCCAGTGATCGTCCATCAATGCCACTTTGCCGTGGAGCGGGCGGCGGCGGTACTC‑AAAAACCTGAAcgcc                                                                                                                                                     <  1:4504152/150‑1 (MQ=255)
      cGATCGTGGATGATGACATTTGCTTCGAGATTCAGTGACAAACTGAGCGGATCGAGATTACTGGACCCTACTGTCGCCCAGTGATCGTCCATCAATGCCACTTTGCCGTGGAGCGGGCGGCGGCGGTACTC‑AAAAACCTGAACGCCGCCt                                                                                                                                                 >  2:463001/1‑150 (MQ=255)
      cGATCGTGGATGATGACATTTGCTTCGAGATTCAGTGACAAACTGAGCGGATCGAGATTACTGGACCCTACTGTCGCCCAGTGATCGTCCATCAATGCCACTTTGCCGTGGAGCGGGCGGCGGCGGTACTC‑AAAAACCTGAACGCCGCCt                                                                                                                                                 >  2:790207/1‑150 (MQ=255)
      cGATCGTGGATGATGACATTTGCTTCGAGATTCAGTGACAAACTGAGCGGATCGAGATTACTGGACCCTACTGTCGCCCAGTGATCGTCCATCAATGCCACTTTGCCGTGGAGCGGGCGGCGGCGGTACTC‑AAAAACCTGAACGCCGCCt                                                                                                                                                 <  2:3163688/150‑1 (MQ=255)
        aTCGTGGATGATGACATTTGCTTCGAGATTCAGTGACAAACTGAGCGGATCGAGATTACTGGACCCTACTGTCGCCCAGTGATCGTCCATCAATGCCACTTTGCCGTGGAGCGGGCGGCGGCGGTACTC‑AAAAACCTGAACTCCGCCttt                                                                                                                                               >  2:1872880/1‑150 (MQ=255)
        aTCGTGGATGATGACATTTGCTTCGAGATTCAGTGACAAACTGAGCGGATCGAGATTACTGGACCCTACTGTCGCCCAGTGATCGTCCATCAATGCCACTTTGCCGTGGAGCGGGCGGCGGCGGTACTC‑AAAAACCTGAACGCCGCCttt                                                                                                                                               <  1:3414418/150‑1 (MQ=255)
          cGTGGATGATGACATTTGCTTCGAGATTCAGTGACAAACTGAGCGGATCGAGATTACTGGACCCTACTGTCGCCCAGTGATCGTCCATCAATGCCACTTTGCCGTGGAGCGGGCGGCGGCGGTACTC‑AAAAACCTGAACTCCGCCTTTaa                                                                                                                                             >  2:3591879/1‑150 (MQ=255)
           gTGGATGATGACATTTGCTTCGAGATTCAGTGACAAACTGAGCGGATCGAGATTACTGGACCCTACTGTCGCCCAGTGATCGTCCATCAATGCCACTTTGCCGTGGAGCGGGCGGCGGCGGTACTC‑AAAAACCTGAACGCCGCCTTTAAc                                                                                                                                            >  2:2327525/1‑150 (MQ=255)
           gTGGATGATGACATTTGCTTCGAGATTCAGTGACAAACTGAGCGGATCGAGATTACTGGACCCTACTGTCGCCCAGTGATCGTCCATCAATGCCACTTTGCCGTGGAGCGGGCGGCGGCGGTACTC‑AAAAACCTGAACGCCGCCTTTAAc                                                                                                                                            <  2:3868901/150‑1 (MQ=255)
           gTGGATGATGACATTTGCTTCGAGATTCAGTGACAAACTGAGCGGATCGAGATTACTGGACCCTACTGTCGCCCAGTGATCGTCCATCAATGCCACTTTGCCGTGGAGCGGGCGGCGGCGGTACTC‑AAAAACCTGAACGCCGCCTTTAAc                                                                                                                                            >  2:1461635/1‑150 (MQ=255)
            tGGATGATGACATTTGCTTCGAGATTCAGTGACAAACTGAGCGGATCGAGATTACTGGACCCTACTGTCGCCCAGTGATCGTCCATCAATGCCACTTTGCCGTGGAGCGGGCGGCGGCGGTACTC‑AAAAACCTGAACGCCGCCTTTAAcc                                                                                                                                           <  1:2458164/150‑1 (MQ=255)
             ggATGATGACATTTGCTTCGAGATTCAGTGACAAACTGAGCGGATCGAGATTACTGGACCCTACTGTCGCCCAGTGATCGTCCATCAATGCCACTTTGCCGTGGAGCGGGCGGCGGCGGTACTC‑AAAAACCTGAACGCCGCCTTTAACCa                                                                                                                                          <  1:3701143/150‑1 (MQ=255)
              gatgatGACATTTGCTTCGAGATTCAGTGACAAACTGAGCGGATCGAGATTACTGGACCCTACTGTCGCCCAGTGATCGTCCATCAATGCCACTTTGCCGTGGAGCGGGCGGCGGCGGTACTC‑AAAAACCTGAACGCCGCCTTTAACCAg                                                                                                                                         <  2:4902329/150‑1 (MQ=255)
                tgatgaCATTTGCTTCGAGATTCAGTGACAAACTGAGCGGATCGAGATTACTGGACCCTACTGTCGCCCAGTGATCGTCCATCAATGCCACTTTGCCGTGGAGCGGGCGGCGGCGGTACTC‑AAAAACCTGAACGCCGCCTTTAACCAGAt                                                                                                                                       >  1:1624185/1‑150 (MQ=255)
                tgatgaCATTTGCTTCGAGATTCAGTGACAAACTGAGCGGATCGAGATTACTGGACCCTACTGTCGCCCAGTGATCGTCCATCAATGCCACTTTGCCGTGGAGCGGGCGGCGGCGGTACTC‑AAAAACCTGAACGCCGCCTTTAACCAGAt                                                                                                                                       <  2:4180650/150‑1 (MQ=255)
                   tgaCATTTGCTTCGAGATTCAGTGACAAACTGAGCGGATCGAGATTACTGGACCCTACTGTCGCCCAGTGATCGTCCATCAATGCCACTTTGCCGTGGAGCGGGCGGCGGCGGTACTC‑AAAAACCTGAACTCCGCCTTTAACCAGATAGt                                                                                                                                    >  2:1818663/1‑150 (MQ=255)
                   tgaCATTTGCTTCGAGATTCAGTGACAAACTGAGCGGATCGAGATTACTGGACCCTACTGTCGCCCAGTGATCGTCCATCAATGCCACTTTGCCGTGGAGCGGGCGGCGGCGGTACTC‑AAAAACCTGAACTCCGCCTTTAACCAGATAGt                                                                                                                                    >  1:3864461/1‑150 (MQ=255)
                   tgaCATTTGCTTCGAGATTCAGTGACAAACTGAGCGGATCGAGATTACTGGACCCTACTGTCGCCCAGTGATCGTCCATCAATGCCACTTTGCCGTGGAGCGGGCGGCGGCGGTACTC‑AAAAACCTGAACGCCGCCTTTAACCAGATAGt                                                                                                                                    <  2:5128836/150‑1 (MQ=255)
                    gaCATTTGCTTCGAGATTCAGTGACAAACTGAGCGGATCGAGATTACTGGACCCTACTGTCGCCCAGTGATCGTCCATCAATGCCACTTTGCCGTGGAGCGGGCGGCGGCGGTACTC‑AAAAACCTGAACGCCGCCTTTAACCAGATAGtt                                                                                                                                   >  1:183201/1‑150 (MQ=255)
                    gaCATTTGCTTCGAGATTCAGTGACAAACTGAGCGGATCGAGATTACTGGACCCTACTGTCGCCCAGTGATCGTCCATCAATGCCACTTTGCCGTGGAGCGGGCGGCGGCGGTACTC‑AAAAACCTGAACGCCGCCTTTAACCAGATAGtt                                                                                                                                   >  1:1195412/1‑150 (MQ=255)
                    gaCATTTGCTTCGAGATTCAGTGACAAACTGAGCGGATCGAGATTACTGGACCCTACTGTCGCCCAGTGATCGTCCATCAATGCCACTTTGCCGTGGAGCGGGCGGCGGCGGTACTC‑AAAAACCTGAACGCCGCCTTTAACCAGATAGtt                                                                                                                                   <  2:1730853/150‑1 (MQ=255)
                     aCATTTGCTTCGAGATTCAGTGACAAACTGAGCGGATCGAGATTACTGGACCCTACTGTCGCCCAGTGATCGTCCATCAATGCCACTTTGCCGTGGAGCGGGCGGCGGCGGTACTC‑AAAAACCTGAACGCCGCCTTTAACCAGATAGTta                                                                                                                                  <  2:4647037/150‑1 (MQ=255)
                       aTTTGCTTCGAGATTCAGTGACAAACTGAGCGGATCGAGATTACTGGACCCTACTGTCGCCCAGTGATCGTCCATCAATGCCACTTTGCCGTGGAGCGGGCGGCGGCGGTACTC‑AAAAACCTGAACGCCGCCTTTAACCAGATAGTtata                                                                                                                                <  1:1027785/150‑1 (MQ=255)
                         ttGCTTCGAGATTCAGTGACAAACTGAGCGGATCGAGATTACTGGACCCTACTGTCGCCCAGTGATCGTCCATCAATGCCACTTTGCCGTGGAGCGGGCGGCGGCGGTACTC‑AAAAACCTGAACGCCGCCTTTAACCAGATAGTTATACa                                                                                                                              <  2:3506448/150‑1 (MQ=255)
                           gCTTCGAGATTCAGTGACAAACTGAGCGGATCGAGATTACTGGACCCTACTGTCGCCCAGTGATCGTCCATCAATGCCACTTTGCCGTGGAGCGGGCGGCGGCGGTACTC‑AAAAACCTGAACTCCGCCTTTAACCAGATAGTTATACAGc                                                                                                                            >  2:1323765/1‑150 (MQ=255)
                             ttCGAGATTCAGTGACAAACTGAGCGGATCGAGATTACTGGACCCTACTGTCGCCCAGTGATCGTCCATCAATGCCACTTTGCCGTGGAGCGGGCGGCGGCGGTACTC‑AAAAACCTGAACGCCGCCTTTAACCAGATAGTTATACAGCac                                                                                                                          >  2:1452994/1‑149 (MQ=255)
                             ttCGAGATTCAGTGACAAACTGAGCGGATCGAGATTACTGGACCCTACTGTCGCCCAGTGATCGTCCATCAATGCCACTTTGCCGTGGAGCGGGCGGCGGCGGTACTC‑AAAAACCTGAACGCCGCCTTTAACCAGATAGTTATACAGCaa                                                                                                                          >  1:3637757/1‑150 (MQ=255)
                             ttCGAGATTCAGTGACAAACTGAGCGGATCGAGATTACTGGACCCTACTGTCGCCCAGTGATCGTCCATCAATGCCACTTTGCCGTGGAGCGGGCGGCGGCGGTACTC‑AAAAACCTGAACGCCGCCTTTAACCAGATAGTTATACAGCaa                                                                                                                          <  1:1317416/150‑1 (MQ=255)
                             ttCGAGATTCAGTGACAAACTGAGCGGATCGAGATTACTGGACCCTACTGTCGCCCAGTGATCGTCCATCAATGCCACTTTGCCGTGGAGCGGGCGGCGGCGGTACTC‑AAAAACCTGAACGCCGCCTTTAACCAGATAGTTATACAGCaa                                                                                                                          <  1:2644144/150‑1 (MQ=255)
                              tCGAGATTCAGTGACAAACTGAGCGGATCGAGATTCCTGGACCCTACTGTCGCCCAGTGATCGTCCATCAATGCCACTTTGCCGTGGAGCGGGCGGCGGCGGTACTC‑AAAAACCTGAACGCCGCCTTTAACCAGATAGTTATACAGCAAg                                                                                                                         <  1:3030963/150‑1 (MQ=255)
                               cGAGATTCAGTGACAAACTGAGCGGATCGAGATTACTGGACCCTACTGTCGCCCAGTGATCGTCCATCAATGCCACTTTGCCGTGGAGCGGGCGGCGGCGGTACTC‑AAAAACCTGAACGCCGCCTTTAACCAGATAGTTATACAGCAAgc                                                                                                                        <  1:3467059/150‑1 (MQ=255)
                               cGAGATTCAGTGACAAACTGAGCGGATCGAGATTACTGGACCCTACTGTCGCCCAGTGATCGTCCATCAATGCCACTTTGCCGTGGAGCGGGCGGCGGCGGTACTC‑AAAAACCTGAACGCCGCCTTTAACCAGATAGTTATACAGCAAgc                                                                                                                        >  1:4124795/1‑150 (MQ=255)
                                gagaTTCAGTGACAAACTGAGCGGATCGAGATTACTGGACCCTACTGTCGCCCAGTGATCGTCCATCAATGCCACTTTGCCGTGGAGCGGGCGGCGGCGGTACTC‑AAAAACCTGAACGCCGCCTTTAACCAGATAGTTATACAGCAAgcg                                                                                                                       >  2:3781003/1‑150 (MQ=255)
                                  gaTTCAGTGACAAACTGAGCGGATCGAGATTACTGGACCCTACTGTCGCCCAGTGATCGTCCATCAATGCCACTTTGCCGTGGAGCGGGCGGCGGCGGTACTC‑AAAAACCTGAACGCCGCCTTTAACCAGATAGTTATACAGCAAgcgcg                                                                                                                     >  2:1532608/1‑150 (MQ=255)
                                     tCAGTGACAAACTGAGCGGATCGAGATTACTGGACCCTACTGTCGCCCAGTGATCGTCCATCAATGCCACTTTGCCGTGGAGCGGGCGGCGGCGGTACTC‑AAAAACCTGAACGCCGCCTTTAACCAGATAGTTATACAGCAAGCGCGCAc                                                                                                                  <  1:4664804/150‑1 (MQ=255)
                                       aGTGACAAACTGAGCGGATCGAGATTACTGGACCCTACTGTCGCCCAGTGATCGTCCATCAATGCCACTTTGCCGTGGAGCGGGCGGCGGCGGTACTC‑AAAAACCTGAACTCCGCCTTTAACCAGATAGTTATACAGCAAGCGCGCACCg                                                                                                                >  2:2599238/1‑150 (MQ=255)
                                       aGTGACAAACTGAGCGGATCGAGATTACTGGACCCTACTGTCGCCCAGTGATCGTCCATCAATGCCACTTTGCCGTGGAGCGGGCGGCGGCGGTACTC‑AAAAACCTGAACGCCGCCTTTAACCAGATAGTTATACAGCAAGCGCGCACCg                                                                                                                >  1:2355452/1‑150 (MQ=255)
                                        gTGACAAACTGAGCGGATCGAGATTACTGGACCCTACTGTCGCCCAGTGATCGTCCATCAATGCCACTTTGCCGTGGAGCGGGCGGCGGCGGTACTC‑AAAAACCTGAACGCCGCCTTTAACCAGATAGTTATACAGCAAGCGCGCACCGa                                                                                                               <  1:2679404/150‑1 (MQ=255)
                                          gACAAACTGAGCGGATCGAGATTACTGGACCCTACTGTCGCCCAGTGATCGTCCATCAATGCCACTTTGCCGTGGAGCGGGCGGCGGCGGTACTC‑AAAAACCTGAACGCCGCCTTTAACCAGATAGTTATACAGCAAGCGCGCACCGAct                                                                                                             >  2:4410179/1‑150 (MQ=255)
                                            cAAACTGAGCGGATCGAGATTACTGGACCCTACTGTCGCCCAGTGATCGTCCATCAATGCCACTTTGCCGTGGAGCGGGCGGCGGCGGTACTC‑AAAAACCTGAACGCCGCCTTTAACCAGATAGTTATACAGCAAGCGCGCACCGActct                                                                                                           >  1:2813824/1‑150 (MQ=255)
                                               aCTGAGCGGATCGAGATTACTGGACCCTACTGTCGCCCAGTGATCGTCCATCAATGCCACTTTGCCGTGGAGCGGGCGGCGGCGGTACTC‑AAAAACCTGAACGCCGCCTTTAACCAGATAGTTATACAGCAAGCGCGCACCGACTCTGAc                                                                                                        >  2:196820/1‑150 (MQ=255)
                                               aCTGAGCGGATCGAGATTACTGGACCCTACTGTCGCCCAGTGATCGTCCATCAATGCCACTTTGCCGTGGAGCGGGCGGCGGCGGTACTC‑AAAAACCTGAACGCCGCCTTTAACCAGATAGTTATACAGCAAGCGCGCACCGACTCTGAc                                                                                                        >  2:4551979/1‑150 (MQ=255)
                                                   aGCGGATCGAGATTACTGGACCCTACTGTCGCCCAGTGATCGTCCATCAATGCCACTTTGCCGTGGAGCGGGCGGCGGCGGTACTC‑AAAAACCTGAACTCCGCCTTTAACCAGATAGTTATACAGCAAGCGCGCACCGACTCTGACAATc                                                                                                    >  2:2544973/1‑150 (MQ=255)
                                                   aGCGGATCGAGATTACTGGACCCTACTGTCGCCCAGTGATCGTCCATCAATGCCACTTTGCCGTGGAGCGGGCGGCGGCGGTACTC‑AAAAACCTGAACGCCGCCTTTAACCAGATAGTTATACAGCAAGCGCGCACCGACTCTGACAATc                                                                                                    <  2:2860577/150‑1 (MQ=255)
                                                    gCGGATCGAGATTACTGGACCCTACTGTCGCCCAGTGATCGTCCATCAATGCCACTTTGCCGTGGAGCGGGCGGCGGCGGTACTC‑AAAAACCTGAACGCCGCCTTTAACCAGATAGTTATACAGCAAGCGCGCACCGACTCTGACAATCg                                                                                                   <  2:3104287/150‑1 (MQ=255)
                                                     cGGATCGAGATTACTGGACCCTACTGTCGCCCAGTGATCGTCCATCAATGCCACTTTGCCGTGGAGCGGGCGGCGGCGGTACTC‑AAAAACCTGAACTCCGCCTTTAACCAGATAGTTATACAGCAAGCGCGCACCGACTCTGACAATCgg                                                                                                  <  1:47995/150‑1 (MQ=255)
                                                     cGGATCGAGATTACTGGACCCTACTGTCGCCCAGTGATCGTCCATCAATGCCACTTTGCCGTGGAGCGGGCGGCGGCGGTACTC‑AAAAACCTGAACGCCGCCTTTAACCAGATAGTTATACAGCAAGCGCGCACCGACTCTGACAATCgg                                                                                                  <  2:3744341/150‑1 (MQ=255)
                                                     cGGATCGAGATTACTGGACCCTACTGTCGCCCAGTGATCGTCCATCAATGCCACTTTGCCGTGGAGCGGGCGGCGGCGGTACTC‑AAAAACCTGAACGCCGCCTTTAACCAGATAGTTATACAGCAAGCGCGCACCGACTCTGACAATCgg                                                                                                  <  1:909231/150‑1 (MQ=255)
                                                      ggATCGAGATTCCTGGACCCGACTGTCGCCCAGTGATCGTCCATCAATGCCACATTGCCGTGGAGCGGGCGGCGGCGGTACTC‑AAAAACCTGAACGCCGCCTTTAACCAGATAGTTATACAGCAAGCGCGCACCGACTCTGACAATCGGc                                                                                                 <  2:5076055/150‑1 (MQ=255)
                                                        aTCGAGATTACTGGACCCTACTGTCGCCCAGTGATCGTCCATCAATGCCACTTTGCCGTGGAGCGGGCGGCGGCGGTACTC‑AAAAACCTGAACGCCGCCTTTAACCAGATAGTTATACAGCAAGCGCGCACCGACTCTGACAATCGGCat                                                                                               <  2:2279067/150‑1 (MQ=255)
                                                         tCGAGATTACTGGACCCTACTGTCGCCCAGTGATCGTCCATCAATGCCACTTTGCCGTGGAGCGGGCGGCGGCGGTACTC‑AAAAACCTGAACTCCGCCTTTAACCAGATAGTTATACAGCAAGCGCGCACCGACTCTGACAATCGGCata                                                                                              >  2:925929/1‑150 (MQ=255)
                                                          cGAGATTACTGGACCCTACTGTCGCCCAGTGATCGTCCATCAATGCCACTTTGCCGTGGAGCGGGCGGCGGCGGTACTC‑AAAAACCTGAACTCCGCCTTTAACCAGATAGTTATACAGCAAGCGCGCACCGACTCTGACAATCGGCatat                                                                                             >  2:4282576/1‑150 (MQ=255)
                                                            agaTTACTGGACCCTACTGTCGCCCAGTGATCGTCCATCAATGCCACTTTGCCGTGGAGCGGGCGGCGGCGGTACTC‑AAAAACCTGAACTCCGCCTTTAACCAGATAGTTATACAGCAAGCGCGCACCGACTCTGACAATCGGCATATcc                                                                                           <  1:2238715/150‑1 (MQ=255)
                                                                 aCTGGACCCTACTGTCGCCCAGTGATCGTCCATCAATGCCACTTTGCCGTGGAGCGGGCGGCGGCGGTACTC‑AAAAACCTGAACGCCGCCTTTAACCAGATAGTTATACAGCAAGCGCGCACCGACTCTGACAATCGGCATATCCGGTTc                                                                                      <  1:4016242/150‑1 (MQ=255)
                                                                       cccTACTGTCGCCCAGTGATCGTCCATCAATGCCACTTTGCCGTGGAGCGGGCGGCGGCGGTACTC‑AAAAACCTGAACGCCGCCTTTAACCAGATAGTTATACAGCAAGCGCGCACCGACTCTGACAATCGGCATATCCGGTTCGCCCTg                                                                                >  1:231391/1‑150 (MQ=255)
                                                                       cccTACTGTCGCCCAGTGATCGTCCATCAATGCCACTTTGCCGTGGAGCGGGCGGCGGCGGTACTC‑AAAAACCTGAACGCCGCCTTTAACCAGATAGTTATACAGCAAGCGCGCACCGACTCTGACAATCGGCATATCCGGTTCGCCCTg                                                                                >  1:4928570/1‑150 (MQ=255)
                                                                            cTGTCGCCCAGTGATCGTCCATCAATGCCACTTTGCCGTGGAGCGGGCGGCGGCGGTACTC‑AAAAACCTGAACGCCGCCTTTAACCAGATAGTTATACAGCAAGCGCGCACCGACTCTGACAATCGGCATATCCGGTTCGCCCTGAATGa                                                                           <  1:112542/150‑1 (MQ=255)
                                                                               tCGCCCAGTGATCGTCCATCAATGCCACTTTGCCGTGGAGCGGGCGGCGGCGGTACTC‑AAAAACCTGAACGCCGCCTTTAACCAGATAGTTATACAGCAAGCGCGCACCGACTCTGACAATCGGCATATCCGGTTCGCCCTGAATGATCa                                                                        <  2:2580546/150‑1 (MQ=255)
                                                                               tCGCCCAGTGATCGTCCATCAATGCCACTTTGCCGTGGAGCGGGCGGCGGCGGTACTC‑AAAAACCTGAACGCCGCCTTTAACCAGATAGTTATACAGCAAGCGCGCACCGACTCTGACAATCGGCATATCCGGTTCGCCCTGAATGATCa                                                                        <  2:4270335/150‑1 (MQ=255)
                                                                                cGCCCAGTGATCGTCCATCAATGCCACTTTGCCGTGGAGCGGGCGGCGGCGGTACTC‑AAAAACCTGAACTCCGCCTTTAACCAGATAGTTATACAGCAAGCGCGCACCGACTCTGACAATCGGCATATCCGGTTCGCCCTGAATGATCAg                                                                       >  1:3599140/1‑150 (MQ=255)
                                                                                 gCCCAGTGATCGTCCATCAATGCCACTTTGCCGTGGAGCGGGCGGCGGCGGTACTC‑AAAAACCTGAACTCCGCCTTTAACCAGATAGTTATACAGCAAGCGCGCACCGACTCTGACAATCGGCATATCCGGTTCGCCCTGAATGATCAGt                                                                      >  1:301886/1‑150 (MQ=255)
                                                                                    cAGTGATCGTCCATCAATGCCACTTTGCCGTGGAGCGGGCGGCGGCGGTACTC‑AAAAACCTGAACTCCGCCTTTAACCAGATAGTTATACAGCAAGCGCGCACCGACTCTGACAATCGGCATATCCGGTTCGCCCTGAATGATCAGTTTg                                                                   <  2:1748931/150‑1 (MQ=255)
                                                                                     aGTGATCGTCCATCAATGCCACTTTGCCGTGGAGCGGGCGGCGGCGGTACTC‑AAAAACCTGAACGCCGCCTTTAACCAGATAGTTATACAGCAAGCGCGCACCGACTCTGACAATCGGCATATCCGGTTCGCCCTGAATGATCAGTTTGa                                                                  <  2:1359049/150‑1 (MQ=255)
                                                                                      gTGATCGTCCATCAATGCCACTTTGCCGTGGAGCGGGCGGCGGCGGTACTCAAAAAACCTGAACGCCGCCTTTAACCAGATAGTTATACAGCAAGCGCGCACCGACTCTGACAATCGGCATATCCGGTTCGCCCTGAATGATCAGTTTGa                                                                  >  1:153194/1‑150 (MQ=255)
                                                                                        gATCGTCCATCAATGCCACTTTGCCGTGGAGCGGGCGGCGGCGGTACTC‑AAAAACCTGAACGCCGCCTTTAACCAGATAGTTATACAGCAAGCGCGCACCGACTCTGACAATCGGCATATCCGGTTCGCCCTGAATGATCAGTTTGATcc                                                               <  2:1421862/150‑1 (MQ=255)
                                                                                        gATCGTCCATCAATGCCACTTTGCCGTGGAGCGGGCGGCGGCGGTACTC‑AAAAACCTGAACGCCGCCTTTAACCAGATAGTTATACAGCAAGCGCGCACCGACTCTGACAATCGGCATATCCGGTTCGCCCTGAATGATCAGTTTGATcc                                                               <  2:4942168/150‑1 (MQ=255)
                                                                                         aTCGTCCATCAATGCCACTTTGCCGTGGAGCGGGCGGCGGCGGTACTC‑AAAAACCTGAACGCCGCCTTTAACCAGATAGTTATACAGCAAGCGCGCACCGACTCTGACAATCGGCATATCCGGTTCGCCCTGAATGATCAGTTTGATCCg                                                              <  2:1197803/150‑1 (MQ=255)
                                                                                           cGTCCATCAATGCCACTTTGCCGTGGAGCGGGCGGCGGCGGTACTC‑AAAAACCTGAACGCCGCCTTTAACCAGATAGTTATACAGCAAGCGCGCACCGACTCTGACAATCGGCATATCCGGTTCGCCCTGAATGATCAGTTTGATCCGCa                                                            >  1:1552520/1‑150 (MQ=255)
                                                                                            gTCCATCAATGCCACTTTGCCGTGGAGCGGGCGGCGGCGGTACTC‑AAAAACCTGAACGCCGCCTTTAACCAGATAGTTATACAGCAAGCGCGCACCGACTCTGACAATCGGCATATCCGGTTCGCCCTGAATGATCAGTTTGATCCGCAc                                                           >  2:3526217/1‑150 (MQ=255)
                                                                                              ccATCAATGCCACTTTGCCGTGGAGCGGGCGGCGGCGGTACTC‑AAAAACCTGAACGCCGCCTTTAACCAGATAGTTATACAGCAAGCGCGCACCGACTCTGACAATCGGCATATCCGGTTCGCCCTGAATGATCAGTTTGATCCGCAccc                                                         >  2:2514086/1‑150 (MQ=255)
                                                                                                   aaTGCCACTTTGCCGTGGAGCGGGCGGCGGCGGTACTC‑AAAAACCTGAACTCCGCCTTTAACCAGATAGTTATACAGCAAGCGCGCACCGACTCTGACAATCGGCATATCCGGTTCGCCCTGAATGATCAGTTTGATCCGCACCCCgcgc                                                    >  2:689461/1‑150 (MQ=255)
                                                                                                    aTGCCACTTTGCCGTGGAGCGGGCGGCGGCGGTACTC‑AAAAACCTGAACGCCGCCTTTAACCAGATAGTTATACAGCAAGCGCGCACCGACTCTGACAATCGGCATATCCGGTTCGCCCTGAATGATCAGTTTGATCCGCACCCCGCGcc                                                   >  1:4351154/1‑150 (MQ=255)
                                                                                                      gCCACTTTGCCGTGGAGCGGGCGGCGGCGGTACTC‑AAAAACCTGAACTCCGCCTTTAACCAGATAGTTATACAGCAAGCGCGCACCGACTCTGACAATCGGCATATCCGGTTCGCCCTGAATGATCAGTTTGATCCGCACCCCGCGCCGt                                                 <  1:192166/150‑1 (MQ=255)
                                                                                                      gCCACTTTGCCGTGGAGCGGGCGGCGGCGGTACTC‑AAAAACCTGAACGCCGCCTTTAACCAGATAGTTATACAGCAAGCGCGCACCGACTCTGACAATCGGCATATCCGGTTCGCCCTGAATGATCAGTTTGATCCGCACCCCGCGCCGt                                                 <  2:3383587/150‑1 (MQ=255)
                                                                                                       ccACTTTGCCGTGGAGCGGGCGGCGGCGGTACTC‑AAAAACCTGAACTCCGCCTTTAACCAGATAGTTATACAGCAAGCGCGCACCGACTCTGACAATCGGCATATCCGGTTCGCCCTGAATGATCAGTTTGATCCGCACCCCGCGCCGTg                                                >  1:4982470/1‑150 (MQ=255)
                                                                                                           tttGCCGTGGAGCGGGCGGCGGCGGTACTC‑AAAAACCTGAACGCCGCCTTTAACCAGATAGTTATACAGCAAGCGCGCACCGACTCTGACAATCGGCATATCCGGTTCGCCCTGAATGATCAGTTTGATCCGCACCCCGCGCCGTGCCGc                                            <  2:4053257/150‑1 (MQ=255)
                                                                                                            ttGCCGTGGAGCGGGCGGCGGCGGTACTC‑AAAAACCTGAACGCCGCCTTTAACCAGATAGTTATACAGCAAGCGCGCACCGACTCTGACAATCGGCATATCCGGTTCGCCCTGAATGATCAGTTTGATCCGCACCCCGCGCCGTGCCGCt                                           <  1:2566276/150‑1 (MQ=255)
                                                                                                                   ggAGCGGGCGGCGGCGGTACTC‑AAAAACCTGAACGCCGCCTTTAACCAGATAGTTATACAGCAAGCGCGCACCGACTCTGACAATCGGCATATCCGGTTCGCCCTGAATGATCAGTTTGATCCGCACCCCGCGCCGTGCCGCTTTACGCa                                    >  2:5007947/1‑150 (MQ=255)
                                                                                                                       cgggcggCGGCGGTACTC‑AAAAACCTGAACGCCGCCTTTAACAAGATAGTTATACAGCAAGCGCGCACCGACTCTGACAATCGGCATATCCGGTTCGCCCTGAATGATCAGTTTGATCCGCACCCCGCGCCGTGCCGCTTTACGCAAGGc                                >  1:5133696/1‑150 (MQ=255)
                                                                                                                        gggcggCGGCGGTACTC‑AAAAACCTGAACGCCGCCTTTAACCAGATAGTTATACAGCAAGCGCGCACCGACTCTGACAATCGGCATATCCGGTTCGCCCTGAATGATCAGTTTGATCCGCACCCCGCGCCGTGCCGCTTTACGCAAGGCg                               >  2:1082053/1‑150 (MQ=255)
                                                                                                                         ggcggcggcGGTACTC‑AAAAACCTGAACGCCGCCTTTAACCAGATAGTTATACAGCAAGCGCGCACCGACTCTGACAATCGGCATATCCGGTTCGCCCTGAATGATCAGTTTGATCCGCACCCCGCGCCGTGCCGCTTTACGCAAGGCgt                              >  2:859268/1‑150 (MQ=255)
                                                                                                                          gcggcggcgGTACTC‑AAAAACCTGAACGCCGCCTTTAACCAGATAGTTATACAGCAAGCGCGCACCGACTCTGACAATCGGCATATCCGGTTCGCCCTGAATGATCAGTTTGATCCGCACCCCGCGCCGTGCCGCTTTACGCAAGGCgtg                             <  1:4551979/150‑1 (MQ=255)
                                                                                                                           cggcggcggTACTC‑AAAAACCTGAACGCCGCCTTTAACCAGATAGTTATACAGCAAGCGCGCACCGACTCTGACAATCGGCATATCCGGTTCGCCCTGAATGATCAGTTTGATCCGCACCCCGCGCCGTGCCGCTTTACGCAAGGCgtgt                            >  2:3828974/1‑150 (MQ=255)
                                                                                                                            ggcggcggTACTC‑AAAAACCTGAACTCCGCCTTTAACCAGATAGTTATACAGCAAGCGCGCACCGACTCTGACAATCGGCATATCCGGTTCGCCCTGAATGATCAGTTTGATCCGCACCCCGCGCCGTGCCGCTTTACGCAAGGCGTGTa                           <  1:825989/150‑1 (MQ=255)
                                                                                                                                  ggTACTC‑AAAAACCTGAACGCCGCCTTTAACCAGATAGTTATACAGCAAGCGCGCACCGACTCTGACAATCGGCATATCCGGTTCGCCCTGAATGATCAGTTTGATCCGCACCCCGCGCCGTGCCGCTTTACGCAAGGCGTGTAAAAATc                     >  1:4249867/1‑150 (MQ=255)
                                                                                                                                    tACTC‑AAAAACCTGAACTCCGCCTTTAACCAGATAGTTATACAGCAAGCGCGCACCGACTCTGACAATCGGCATATCCGGTTCGCCCTGAATGATCAGTTTGATCCGCACCCCGCGCCGTGCCGCTTTACGCAAGGCGTGTAAAAATCGa                   >  2:2590803/1‑150 (MQ=255)
                                                                                                                                       tC‑AAAAACCTGAACGCCGCCTTTAACCAGATAGTTATACAGCAAGCGCGCACCGACTCTGACAATCGGCATATCCGGTTCGCCCTGAATGATCAGTTTGATCCGCACCCCGCGCCGTGCCGCTTTACGCAAGGCGTGTAAAAATCGATAg                <  1:1941683/150‑1 (MQ=255)
                                                                                                                                            aaaCCTGAACTCCGCCTTTAACCAGATAGTTATACAGCAAGCGCGCACCGACTCTGACAATCGGCATATCCGGTTCGCCCTGAATGATCAGTTTGATCCGCACCCCGCGCCGTGCCGCTTTACGCAAGGCGTGTAAAAATCGATAGCCgg            <  2:2217726/150‑1 (MQ=255)
                                                                                                                                              aCCTGAACTCCGCCTTTAACCAGATAGTTATACAGCAAGCGCGCACCGACTCTGACAATCGGCATATCCGGTTCGCCCTGAATGATCAGTTTGATCCGCACCCCGCGCCGTGCCGCTTTACGCAAGGCGTGTAAAAATCGATAGCCgggg          <  1:3104273/150‑1 (MQ=255)
                                                                                                                                                cTGAACGCCGCCTTTAACCAGATAGTTATACAGCAAGCGCGCACCGACTCTGACAATCGGCATATCCGGTTCGCCCTGAATGATCAGTTTGATCCGCACCCCGCGCCGTGCCGCTTTACGCAAGGCGTGTAAAAATCGATAGCCGGGgaa        >  2:2729273/1‑150 (MQ=255)
                                                                                                                                                 tGAACGCCGCCTTTAACCAGATAGTTATACAGCAAGCGCGCACCGACTCTGACACTCGGCATATCCGGTTCGCCCTGAAGGATCAGTGTGATCCGCACCCCGCGCCGTGCCGCTTTACGCAAGGCGTGTAAAAATCGATAGCCGGGgaag       <  2:1602055/150‑1 (MQ=255)
                                                                                                                                                  gAACGCCGCCTTTAACCAGATAGTTATACAGCAAGCGCGCACCGACTCTGACAATCGGCATATCCGGTTCGCCCTGAATGATCAGTTTGATCCGCACCCCGCGCCGTGCCGCTTTACGCAAGGCGTGTAAAAATCGATAGCCGGGgaaga      <  1:2068060/150‑1 (MQ=255)
                                                                                                                                                  gAACGCCGCCTTTAACCAGATAGTTATACAGCAAGCGCGCACCGACTCTGACAATCGGCATATCCGGTTCGCCCTGAATGATCAGTTTGATCCGCACCCCGCGCCGTGCCGCTTTACGCAAGGCGTGTAAAAATCGATAGCCGGGgaaga      <  1:2699671/150‑1 (MQ=255)
                                                                                                                                                     cgccgcCTTTAACCAGATAGTTATACAGCAAGCGCGCACCGACTCTGACAATCGGCATATCCGGTTCGCCCTGAATGATCAGTTTGATCCGCACCCCGCGCCGTGCCGCTTTACGCAAGGCGTGTAAAAATCGATAGCCGGGGAAGAAGt   >  1:2554219/1‑150 (MQ=255)
                                                                                                                                                     cgccgcCTTTAACCAGATAGTTATACAGCAAGCGCGCACCGACTCTGACAATCGGCATATCCGGTTCGCCCTGAATGATCAGTTTGATCCGCACCCCGCGCCGTGCCGCTTTACGCAAGGCGTGTAAAAATCGATAGCCGGGGAAGAAGt   >  2:4737299/1‑150 (MQ=255)
                                                                                                                                                     cgccgcCTTTAACCAGATAGTTATACAGCAAGCGCGCACCGACTCTGACAATCGGCATATCCGGTTCGCCCTGAATGATCAGTTTGATCCGCACCCCGCGCCGTGCCGCTTTACGCAAGGCGTGTAAAAATCGATAGCCGGGGAAGAAGt   <  1:3969430/150‑1 (MQ=255)
                                                                                                                                                      |                                                                                                                                                     
AAATGACGATCGTGGATGATGACATTTGCTTCGAGATTCAGTGACAAACTGAGCGGATCGAGATTACTGGACCCTACTGTCGCCCAGTGATCGTCCATCAATGCCACTTTGCCGTGGAGCGGGCGGCGGCGGTACTC‑AAAAACCTGAACGCCGCCTTTAACCAGATAGTTATACAGCAAGCGCGCACCGACTCTGACAATCGGCATATCCGGTTCGCCCTGAATGATCAGTTTGATCCGCACCCCGCGCCGTGCCGCTTTACGCAAGGCGTGTAAAAATCGATAGCCGGGGAAGAAGTA  >  CP009273/818213‑818511

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑