Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 832,445 G→T 87.6% P213P (CCG→CCT)  ybiC → putative dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273832,4450GT87.6% 795.2 / 110.2 299P213P (CCG→CCT) ybiCputative dehydrogenase
Reads supporting (aligned to +/- strand):  ref base G (14/23);  new base T (129/133);  total (143/156)
Fisher's exact test for biased strand distribution p-value = 2.21e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.77e-01

Only 100 of 314 total aligned reads displayed.

GTTCTGTGTGGTTTTCCCTCGTAAAGATAATTTCCCGCTGTTGCTTGATTACGCCACCAGCGCCATTGCATTTGGCAAAACCCGCGTCGCCTGGCATAAAGGCGTCCCCGTGCCGCCAGGTTGCCTGATTGACGTTAACGGCGTGCCGACGACCAATCCGGCGGTAATGCAGGAGTCGCCGTTGGGTTCGCTGTTGACCTTTGCCGAACATAAAGGCTACGCCCTTGCAGCGATGTGTGAAATTCTTGGCGGGGCGCTTTCCGGCGGTAAAACGACGCATCAGGAAACGTTAC  >  CP009273/832298‑832590
                                                                                                                                                   |                                                                                                                                                 
gTTCTGTGTGGTTTTCCCTCGTAAAGATAATTTCCCGCTGTTGCTTGATTACGCCACCAGCGCCATTGCATTTGGCAAAACCCGCGTCGCCTGGCATAAAGGCGTCCCCGTGCCGCCAGGTTGCCTGATTGACGTTAACGGCGTGCctac                                                                                                                                                 >  2:2946937/1‑150 (MQ=255)
  tCTGTGTGGTTTTCCCTCGTAAAGATAATGTCCCGCTGTTGCTTGATTACGCCACCAGCGCCATTGCATTTGGCAAAACCCGCGTCGCCTGGCATAAAGGCGTCCCCGTGCCGCCAGGTTGCCTGATTGACGTTAACGGCGTGCctacga                                                                                                                                               <  2:3482473/150‑1 (MQ=255)
   cTGTGTGGTTTTCCCTCGTAAAGATAATTTCCCGCTGTTGCTTGATTACGCCACCAGCGCCATTGCATTTGGCAAAACCCGCGTCGCCTGGCATAAAGGCGTCCCCGTGCCGCCAGGTTGCCTGATTGACGTTAACGGCGTGCctacg                                                                                                                                                >  1:2512177/1‑148 (MQ=255)
   cTGTGTGGTTTTCCCTCGTAAAGATAATTTCCCGCTGTTGCTTGATTACGCCACCAGCGCCATTGCATTTGGCAAAACCCGCGTCGCCTGGCATAAAGGCGTCCCCGTGCCGCCAGGTTGCCTGATTGACGTTAACGGCGTGCCtacgac                                                                                                                                              <  1:386596/150‑1 (MQ=255)
   cTGTGTGGTTTTCCCTCGTAAAGATAATTTCCCGCTGTTGCTTGATTACGCCACCAGCGCCATTGCATTTGGCAAAACCCGCGTCGCCTGGCATAAAGGCGTCCCCGTGCCGCCAGGTTGCCTGATTGACGTTAACGGCGTGCCtacgac                                                                                                                                              >  2:3802425/1‑150 (MQ=255)
   cTGTGTGGTTTTCCCTCGTAAAGATAATTTCCCGCTGTTGCTTGATTACGCCACCAGCGCCATTGCATTTGGCAAAACCCGCGTCGCCTGGCATAAAGGCGTCCCCGTGCCGCCAGGTTGCCTGATTGACGTTAACGGCGTGCCtacgac                                                                                                                                              >  2:4134918/1‑150 (MQ=255)
       gtgGTTTTCCCTCGTAAAGATAATTTCCCGCTGTTGCTTGATTACGCCACCAGCGCCATTGCATTTGGCAAAACCCGCGTCGCCTGGCATAAAGGCGTCCCCGTGCCGCCAGGTTGCCTGATTGACGTTAACGGCGTGCCGACGACCAAt                                                                                                                                          >  1:1161304/1‑150 (MQ=255)
          gTTTTCCCTCGTAAAGATAATTTCCCGCTGTTGCTTGATTACGCCACCAGCGCCATTGCATTTGGCAAAACCCGCGTCGCCTGGCATAAAGGCGTCCCCGTGCCGCCAGGTTGCCTGATTGACGTTAACGGCGTGCCTACGACCAATCcg                                                                                                                                       >  1:2602410/1‑150 (MQ=255)
            tttCCCTCGTAAAGATAATTTCCCGCTGTTGCTTGATTACGCCACCAGCGCCATTGCATTTGGCAAAACCCGCGTCGCCTGGCATAAAGGCGTCCCCGTGCCGCCAGGTTGCCTGATTGACGTTAACGGCGTGCCTACGACCAATCcggc                                                                                                                                     >  2:1018898/1‑150 (MQ=255)
             ttCCCTCGTAAAGATAATTTCCCGCTGTTGCTTGATTACGCCACCAGCGCCATTGCATTTGGCAAAACCCGCGTCGCCTGGCATAAAGGCGTCCCCGTGCCGCCAGGTTGCCTGATTGACGTTAACGGCGTGCCTACGACCAATCcggcg                                                                                                                                    <  1:919998/150‑1 (MQ=255)
              tCCCTCGTAAAGATAATTTCCCGCTGTTGCTTGATTACGCCACCAGCGCCATTGCATTTGGCAAAACCCGCGTCGCCTGGCATAAAGGCGTCCCCGTGCCGCCAGGTTGCCTGATTGACGTTAACGGCGTGCCTACGACCAATCcggcgg                                                                                                                                   >  2:4001149/1‑150 (MQ=255)
              tCCCTCGTAAAGATAATTTCCCGCTGTTGCTTGATTACGCCACCAGCGCCATTGCATTTGGCAAAACCCGCGTCGCCTGGCATAAAGGCGTCCCCGTGCCGCCAGGTTGCCTGATTGACGTTAACGGCGTGCCTACGACCAATCcggcgg                                                                                                                                   >  1:4416925/1‑150 (MQ=255)
               cccTCGTAAAGATAATTTCCCGCTGTTGCTTGATTACGCCACCAGCGCCATTGCATTTGGCAAAACCCGCGTCGCCTGGCATAAAGGCGTCCCCGTGCCGCCAGGTTGCCTGATTGACGTTAACGGCGTGCCTACGACCAATCCGGCGGt                                                                                                                                  <  1:3969198/150‑1 (MQ=255)
               cccTCGTAAAGATAATTTCCCGCTGTTGCTTGATTACGCCACCAGCGCCATTGCATTTGGCAAAACCCGCGTCGCCTGGCATAAAGGCGTCCCCGTGCCGCCAGGTTGCCTGATTGACGTTAACGGCGTGCCTACGACCAATCCGGCGGt                                                                                                                                  >  1:4747534/1‑150 (MQ=255)
                ccTCGTAAAGATAATTTCCCGCTGTTGCTTGATTACGCCACCAGCGCCATTGCATTTGGCAAAACCCGCGTCGCCTGGCATAAAGGCGTCCCCGTGCCGCCAGGTTGCCTGATTGACGTTAACGGCGTGCCGACGACCAATCCGGCGGTa                                                                                                                                 >  1:2369219/1‑150 (MQ=255)
                  tCGTAAAGATAATTTCCCGCTGTTGCTTGATTACGCCACCAGCGCCATTGCATTTGGCAAAACCCGCGTCGCCTGGCATAAAGGCGTCCCCGTGCCGCCAGGTTGCCTGATTGACGTTAACGGCGTGCCTACGACCAATCCGGCGGTAAt                                                                                                                               <  2:410008/150‑1 (MQ=255)
                   cGTAAAGATAATTTCCCGCTGGTGCTTGATTACGCCACCAGCGCCATTGCATTTGGCAAAACCCGCGTCGCCTGGCATAAAGGCGTCCCCGTGCCGCCAGGTTGCCTGATTGACGTTAACGGCGTGCCTACGACCAATCCGGCGGTAATg                                                                                                                              <  2:2165025/150‑1 (MQ=255)
                    gTAAAGATAATTTCCCGCTGTTGCTTGATTACGCCACCAGCGCCATTGCATTTGGCAAAACCCGCGTCGCCTGGCATAAAGGCGTCCCCGTGCCGCCAGGTTGCCTGATTGACGTTAACGGCGTGCCTACGACCAATCCGGCGGTAATGc                                                                                                                             >  1:131072/1‑150 (MQ=255)
                     tAAAGATAATTTCCCGCTGTTGCTTGATTACGCCACCAGCGCCATTGCATTTGGCAAAACCCGCGTCGCCTGGCATAAAGGCGTCCCCGTGCCGCCCGGTTGCCTGATTGACGTTAACGGCGTGCCTACGACCAATCCGGCGGTAATGCa                                                                                                                            >  2:5771/1‑150 (MQ=255)
                        aGATAATTTCCCGCTGTTGCTTGATTACGCCACCAGCGCCATTGCATTTGGCAAAACCCGCGTCGCCTGGCATAAAGGCGTCCCCGTGCCGCCAGGTTGCCTGATTGACGTTAACGGCGTGCCTACGACCAATCCGGCGGTAATGCAGGa                                                                                                                         <  1:618035/150‑1 (MQ=255)
                        aGATAATTTCCCGCTGTTGCTTGATTACGCCACCAGCGCCATTGCATTTGGCAAAACCCGCGTCGCCTGGCATAAAGGCGTCCCCGTGCCGCCAGGTTGCCTGATTGACGTTAACGGCGTGCCTACGACCAATCCGGCGGTAATGCAGGa                                                                                                                         <  1:342988/150‑1 (MQ=255)
                        aGATAATTTCCCGCTGTTGCTTGATTACGCCACCAGCGCCATTGCATTTGGCAAAACCCGCGTCGCCTGGCATAAAGGCGTCCCCGTGCCGCCAGGTTGCCTGATTGACGTTAACGGCGTGCCTACGACCAATCCGGCGGTAATGCAGGa                                                                                                                         <  2:5012153/150‑1 (MQ=255)
                        aGATAATTTCCCGCTGTTGCTTGATTACGCCACCAGCGCCATTGCATTTGGCAAAACCCGCGTCGCCTGGCATAAAGGCGTCCCCGTGCCGCCAGGTTGCCTGATTGACGTTAACGGCGTGCCTACGACCAATCCGGCGGTAATGCAGGa                                                                                                                         <  1:3626774/150‑1 (MQ=255)
                         gATAATTTCCCGCTGTTGCTTGATTACGCCACCAGCGCCATTGCATTTGGCAAAACCCGCGTCGCCTGGCATAAAGGCGTCCCCGTGCCGCCAGGTTGCCTGATTGACGTTAACGGCGTGCCTACGACCAATCCGGCGGTAATGCAGGAg                                                                                                                        <  2:1983964/150‑1 (MQ=255)
                          aTAATTTCCCGCTGTTGCTTGATTACGCCACCAGCGCCATTGCATTTGGCAAAACCCGCGTCGCCTGGCATAAAGGCGTCCCCGTGCCGCCAGGTTGCCTGATTGACGTTAACGGCGTGCCTACGACCAATCCGGCGGTAATGCAGGAGt                                                                                                                       >  2:4577168/1‑150 (MQ=255)
                               ttCCCGCTGTTGCTTGATTACGCCACCAGCGCCATTGCATTTGGCAAAACCCGCGTCGCCTGGCATAAAGGCGTCCCCGTGCCGCCAGGTTGCCTGATTGACGTTAACGGCGTGCCTACGACCAATCCGGCGGTAATGCAGGAGTCGCCg                                                                                                                  <  2:1319354/150‑1 (MQ=255)
                               ttCCCGCTGTTGCTTGATTACGCCACCAGCGCCATTGCATTTGGCAAAACCCGCGTCGCCTGGCATAAAGGCGTCCCCGTGCCGCCAGGTTGCCTGATTGACGTTAACGGCGTGCCTACGACCAATCCGGCGGTAATGCAGGAGTCGCCg                                                                                                                  <  1:4140930/150‑1 (MQ=255)
                                tCCCGCTGTTGCTTGATTACGCCACCAGCGCCATTGCATTTGGCAAAACCCGCGTCGCCTGGCATAAAGGCGTCCCCGTGCCGCCAGGTTGCCTGATTGACGTTAACGGCGTGCCTACGACCAATCCGGCGGTAATGCAGGAGTCGCCGt                                                                                                                 >  2:1406320/1‑150 (MQ=255)
                                tCCCGCTGTTGCTTGATTACGCCACCAGCGCCATTGCATTTGGCAAAACCCGCGTCGCCTGGCATAAAGGCGTCCCCGTGCCGCCAGGTTGCCTGATTGACGTTAACGGCGGGCCTACGACCAATCCGGCGGTAATGCAGGAGTCGCCGt                                                                                                                 <  2:154033/150‑1 (MQ=255)
                                 cccGCTGTTGCTTGATTACGCCACCAGCGCCATTGCATTTGGCAAAACCCGCGTCGCCTGGCATAAAGGCGTCCCCGTGCCGCCAGGTTGCCTGATTGACGTTAACGGCGTGCCTACGACCAATCCGGCGGTAATGCAGGAGTCGCCGtt                                                                                                                >  1:2831680/1‑150 (MQ=255)
                                  ccGCTGTTGCTTGATTACGCCACCAGCGCCATTGCATTTGGCAAAACCCGCGTCGCCTGGCATAAAGGCGTCCCCGTGCCGCCAGGTTGCCTGATTGACGTTAACGGCGTGCCTACGACCAATCCGGCGGTAATGCAGGAGTCGCCGTTg                                                                                                               <  2:3454232/150‑1 (MQ=255)
                                    gCTGTTGCTTGATTACGCCACCAGCGCCATTGCATTTGGCAAAACCCGCGTCGCCTGGCATAAAGGCGTCCCCGTGCCGCCAGGTTGCCTGATTGACGTTAACGGCGTGCCTACGACCAATCCGGCGGTAATGCAGGAGTCGCCGTTggg                                                                                                             <  1:2925888/150‑1 (MQ=255)
                                     cTGTTGCTTGATTACGCCACCAGCGCCATTGCATTTGGCAAAACCCGCGTCGCCTGGCATAAAGGCGTCCCCGTGCCGCCAGGTTGCCTGATTGACGTTAACGGCGTGCCTACGACCAATCCGGCGGTAATGCAGGAGTCGCCGTTGGGt                                                                                                            <  2:728506/150‑1 (MQ=255)
                                      tGTTGCTTGATTACGCCACCAGCGCCATTGCATTTGGCAAAACCCGCGTCGCCTGGCATAAAGGCGTCCCCGTGCCGCCAGGTTGCCTGATTGACGTTAACGGCGTGCCTACGACCAATCCGGCGGTAATGCAGGAGTCGCCGTTGGGtt                                                                                                           >  2:1284124/1‑150 (MQ=255)
                                           cTTGATTACGCCACCAGCGCCATTGCATTTGGCAAAACCCGCGTCGCCTGGCATACAGGCGTCCCCGTGCCGCCAGGTTGCCTGATTGACGTTAACGGCGTGCCTACGACCAATCCGGCGGTAATGCAGGAGTCGCCGTTGGGTTCGCTg                                                                                                      <  2:2816448/150‑1 (MQ=255)
                                           cTTGATTACGCCACCAGCGCCATTGCATTTGGCAAAACCCGCGTCGCCTGGCATAAAGGCGTCCCCGTGCCGCCAGGTTGCCTGATTGACGTTAACGGCGTGCCTACGACCAATCCGGCGGTAATGCAGGAGTCGCCGTTGGGTTCGCTg                                                                                                      >  2:2863935/1‑150 (MQ=255)
                                            ttGATTACGCCACCAGCGCCATTGCATTTGGCAAAACCCGCGTCGCCTGGCATAAAGGCGTCCCCGTGCCGCCAGGTTGCCTGATTGACGTTAACGGCGTGCCGACGACCAATCCGGCGGTAATGCAGGAGTCGCCGTTGGGTTCGCTGt                                                                                                     >  2:2604800/1‑150 (MQ=255)
                                             tGATTACGCCACCAGCGCCATTGCATTTGGCAAAACCCGCGTCGCCTGGCATAAAGGCGTCCCCGTGCCGCCAGGTTGCCTGATTGACGTTAACGGCGTGCCTACGACCAATCCGGCGGTAATGCAGGAGTCGCCGTTGGGTTCGCTGtt                                                                                                    >  1:1933138/1‑150 (MQ=255)
                                             tGATTACGCCACCAGCGCCATTGCATTTGGCAAAACCCGCGTCGCCTGGCATAAAGGCGTCCCCGTGCCGCCAGGTTGCCTGATTGACGTTAACGGCGTGCCTACGACCAATCCGGCGGTAATGCAGGAGTCGCCGTTGGGTTCGCTGtt                                                                                                    >  1:2989714/1‑150 (MQ=255)
                                              gATTACGCCACCAGCGCCATTGCATTTGGCAAAACCCGCGTCGCCTGGCATAAAGGCGTCCCCGTGCCGCCAGGTTGCCTGATTGACGTTAACGGCGTGCCTACGACCAATCCGGCGGTAATGCAGGAGTCGCCGTTGGGTTCGCTGTTg                                                                                                   >  1:726606/1‑150 (MQ=255)
                                                  aCGCCACCAGCGCCATTGCATTTGGCAAAACCCGCGTCGCCTGGCATAAAGGCGTCCCCGTGCCGCCAGGTTGCCTGATTGACGTTAACGGCGTGCCGACGACCAATCCGGCGGTAATGCAGGAGTCGCCGTTGGGTTCGCTGTTGACCt                                                                                               <  2:2708537/150‑1 (MQ=255)
                                                    gCCACCAGCGCCATTGCATTTGGCAAAACCCGCGTCGCCTGGCATAAAGGCGTCCCCGTGCCGCCAGGTTGCCTGATTGACGTTAACGGCGTGCCTACGACCAATCCGGCGGTAATGCAGGAGTCGCCGTTGGGTTCGCTGTTGACCttt                                                                                             <  2:3162502/150‑1 (MQ=255)
                                                     ccaccaGCGCCATTGCATTTGGCAAAACCCGCGTCGCCTGGCATAAAGGCGTCCCCGTGCCGCCAGGTTGCCTGATTGACGTTAACGGCGTGCCTACGACCAATCCGGCGGTAATGCAGGAGTCGCCGTTGGGTTCGCTGTTGACCTTTg                                                                                            <  2:2849968/150‑1 (MQ=255)
                                                     ccaccaGCGCCATTGCATTTGGCAAAACCCGCGTCGCCTGGCATAAAGGCGTCCCCGTGCCGCCAGGTTGCCTGATTGACGTTAACGGCGTGCCTACGACCAATCCGGCGGTAATGCAGGAGTCGCCGTTGGGTTCGCTGTTGACCTTTg                                                                                            <  2:4815591/150‑1 (MQ=255)
                                                       accaGCGCCATTGCATTTGGCAAAACCCGCGTCGCCTGGCATAAAGGCGTCCCCGTGCCGCCAGGTTGCCTGATTGACGTTAACGGCGTGCCTACGACCAATCCGGCGGTAATGCAGGAGTCGCCGTTGGGTTCGCTGTTGACCTTTGcc                                                                                          >  1:1637943/1‑150 (MQ=255)
                                                        ccaGCGCCATTGCATTTGGCAAAACCCGCGTCGCCTGGCATAAAGGCGTCCCCGTGCCGCCAGGTTGCCTGATTGACGTTAACGGCGTGCCGACGACCAATCCGGCGGTAATGCAGGAGTCGCCGTTGGGTTCGCTGTTGACCTTTGCCg                                                                                         >  1:4277670/1‑150 (MQ=255)
                                                         caGCGCCATTGCATTTGGCAAAACCCGCGTCGCCTGGCATAAAGGCGTCCCCGTGCCGCCAGGTTGCCTGATTGACGTTAACGGCGTGCCTACGACCAATCCGGCGGTAATGCAGGAGTCGCCGTTGGGTTCGCTGTTGACCTTTGCCGa                                                                                        <  2:2241102/150‑1 (MQ=255)
                                                          aGCGCCATTGCATTTGGCAAAACCCGCGTCGCCTGGCATAAAGGCGTCCCCGTGCCGCCAGGTTGCCTGATTGACGTTAACGGCGTGCCTACGACCAATCCGGCGGTAATGCAGGAGTCGCCGTTGGGTTCGCTGTTGACCTTTGCCGaa                                                                                       >  1:1126183/1‑150 (MQ=255)
                                                           gcgcCATTGCATTTGGCAAAACCCGCGTCGCCTGGCATAAAGGCGTCCCCGTGCCGCCAGGTTGCCTGATTGACGTTAACGGCGTGCCTACGACCAATCCGGCGGTAATGCAGGAGTCGCCGTTGGGTTCGCTGTTGACCTTTGCCGAAc                                                                                      <  1:3237927/150‑1 (MQ=255)
                                                            cgcCATTGCATTTGGCAAAACCCGCGTCGCCTGGCATAAAGGCGTCCCCGTGCCGCCAGGTTGCCTGATTGACGTTAACGGCGTGCCTACGACCAATCCGGCGGTAATGCAGGAGTCGCCGTTGGGTTCGCTGTTGACCTTTGCCGAACa                                                                                     >  1:2238774/1‑150 (MQ=255)
                                                             gcCATTGCATTTGGCAAAACCCGCGTCGCCTGGCATAAAGGCGTCCCCGTGCCGCCAGGTTGCCTGATTGACGTTAACGGCGTGCCTACGACCAATCCGGCGGTAATGCAGGAGTCGCCGTTGGGTTCGCTGTTGACCTTTGCCGAACAt                                                                                    >  1:1171517/1‑150 (MQ=255)
                                                               cATTGCATTTGGCAAAACCCGCGTCGCCTGGCATAAAGGCGTCCCCGTGCCGCCAGGTTGCCTGATTGACGTTAACGGCGTGCCTACGACCAATCCGGCGGTAATGCAGGAGTCGCCGTTGGGTTCGCTGTTGACCTTTGCCGAACATaa                                                                                  >  1:1333649/1‑150 (MQ=255)
                                                                aTTGCATTTGGCAAAACCCGCGTCGCCTGGCATAAAGGCGTCCCCGTGCCGCCAGGTTGCCTGATTGACGTTAACGGCGTGCCTACGACCAATCCGGCGGTAATGCAGGAGTCGCCGTTGGGTTCGCTGTTGACCTTTGCCGAACATaaa                                                                                 >  2:3541324/1‑150 (MQ=255)
                                                                 ttGCATTTGGCAAAACCCGCGTCGCCTGGCATAAAGGCGTCCCCGTGCCGCCAGGTTGCCTGATTGACGTTAACGGCGTGCCTACGACCAATCCGGCGGTAATGCAGGAGTCGCCGTTGGGTTCGCTGTTGACCTTTGCCGAACATAAAg                                                                                >  2:1847836/1‑150 (MQ=255)
                                                                  tGCATTTGGCAAAACCCGCGTCGCCTGGCATAAAGGCGTCCCCGTGCCGCCAGGTTGCCTGATTGACGTTAACGGCGTGCCTACGACCAATCCGGCGGTAATGCAGGAGTCGCCGTTGGGTTCGCTGTTGACCTTTGCCGAACATAAAgg                                                                               <  2:327776/150‑1 (MQ=255)
                                                                  tGCATTTGGCAAAACCCGCGTCGCCTGGCATAAAGGCGTCCCCGTGCCGCCAGGTTGCCTGATTGACGTTAACGGCGTGCCTACGACCAATCCGGCGGTAATGCAGGAGTCGCCGTTGGGTTCGCTGTTGACCTTTGCCGAACATAAAgg                                                                               <  2:3684365/150‑1 (MQ=255)
                                                                   gCATTTGGCAAAACCCGCGTCGCCTGGCATAAAGGCGTCCCCGTGCCGCCAGGTTGCCTGATTGACGTTAACGGCGTGCCGACGACCAATCCGGCGGTAATGCAGGAGTCGCCGTTGGGTTCGCTGTTGACCTTTGCCGAACATAAAGGc                                                                              <  2:2804170/150‑1 (MQ=255)
                                                                     aTTTGGCAAAACCCGCGTCGCCTGGCATAAAGGCGTCCCCGTGCCGCCAGGTTGCCTGATTGACGTTAACGGCGTGCCTACGACCAATCCGGCGGTAATGCAGGAGTCGCCGTTGGGTTCGCTGTTGACCTTTGCCGAACATAAAGGCTa                                                                            >  1:4559441/1‑150 (MQ=255)
                                                                       ttGGCAAAACCCGCGTCGCCTGGCATAAAGGCGTCCCCGTGCCGCCAGGTTGCCTGATTGACGTTAACGGCGTGCCTACGACCAATCCGGCGGTAATGCAGGAGTCGCCGTTGGGTTCGCTGTTGACCTTTGCCGAACATAAAGGCTACg                                                                          <  2:1465103/150‑1 (MQ=255)
                                                                       ttGGCAAAACCCGCGTCGCCTGGCATAAAGGCGTCCCCGTGCCGCCAGGTTGCCTGATTGACGTTAACGGCGTGCCTACGACCAATCCGGCGGTAATGCAGGAGTCGCCGTTGGGTTCGCTGTTGACCTTTGCCGAACATAAAGGCTACg                                                                          >  2:1761786/1‑150 (MQ=255)
                                                                         ggCAAAACCCGCGTCGCCTGGCATAAAGGCGTCCCCGTGCCGCCAGGTTGCCTGATTGACGTTAACGGCGTGCCTACGACCAATCCGGCGGTAATGCAGGAGTCGCCGTTGGGTTCGCTGTTGACCTTTGCCGAACATAAAGGCTACGcc                                                                        >  1:3299674/1‑150 (MQ=255)
                                                                         ggCAAAACCCGCGTCGCCTGGCATAAAGGCGTCCCCGTGCCGCCAGGTTGCCTGATTGACGTTAACGGCGTGCCGACGACCAATCCGGCGGTAATGCAGGAGTCGCCGTTGGGTTCGCTGTTGACCTTTGCCGAACATAAAGGCTACGcc                                                                        <  1:608924/150‑1 (MQ=255)
                                                                           cAAAACCCGCGTCGCCTGGCATAAAGGCGTCCCCGTGCCGCCAGGTTGCCTGATTGACGTTAACGGCGTGCCTACGACCAATCCGGCGGTAATGCAGGAGTCGCCGTTGGGTTCGCTGTTGACCTTTGCCGAACATAAAGGCTACGCCCt                                                                      <  1:4290084/150‑1 (MQ=255)
                                                                              aaCCCGCGTCGCCTGGCATAAAGGCGTCCCCGTGCCGCCAGGTTGCCTGATTGACGTTAACGGCGTGCCTACGACCAATCCGGCGGTAATGCAGGAGTCGCCGTTGGGTTCGCTGTTGACCTTTGCCGAACATAAAGGCTACGCCCTTGc                                                                   >  1:3892928/1‑150 (MQ=255)
                                                                                  cgcgTCGCCTGGCATAAAGGCGTCCCCGTGCCGCCAGGTTGCCTGATTGACGTTAACGGCGTGCCTACGACCAATCCGGCGGTAATGCAGGAGTCGCCGTTGGGTTCGCTGTTGACCTTTGCCGAACATAAAGGCTACGCCCTTGCAGCg                                                               >  1:3488127/1‑150 (MQ=255)
                                                                                  cgcgTCGCCTGGCATAAAGGCGTCCCCGTGCCGCCAGGTTGCCTGAGTGACGTTAACGGCGTGCCTACGACCAATCCGGCGGTAATGCAGGAGTCGCCGTTGGGTTCGCTGTTGACCTTTGCCGAACATAAAGGCTACGCCCTTGCAGCg                                                               <  2:4266476/150‑1 (MQ=255)
                                                                                    cgTCGCCTGGCATAAAGGCGTCCCCGTGCCGCCAGGTTGCCTGATTGACGTTAACGGCGTGCCTACGACCAATCCGGCGGTAATGCAGGAGTCGCCGTTGGGTTCGCTGTTGACCTTTGCCGAACATAAAGGCTACGCCCTTGCAGCGAt                                                             >  2:4197888/1‑150 (MQ=255)
                                                                                    cgTCGCCCGGCATAAAGGCGTCCCCGTGCCGCCAGGTTGCCTGATTGACGTTAACGGCGTGCCTACGACCAATCCGGCGGTAATGCAGGAGTCGCCGTTGGGTTCGCTGTTGACCTTTGCCGAACATAAAGGCTACGCCCTTGCAGCGAt                                                             <  1:3572452/150‑1 (MQ=255)
                                                                                      tCGCCTGGCATAAAGGCGTCCCCGTGCCGCCAGGTTGCCTGATTGACGTTAACGGCGTGCCTACGACCAATCCGGCGGTAATGCAGGAGTCGCCGTTGGGTTCGCTGTTGACCTTTGCCGAACATAAAGGCTACGCCCTTGCAGCGAtgt                                                           >  1:207569/1‑150 (MQ=255)
                                                                                      tCGCCTGGCATAAAGGCGTCCCCGTGCCGCCAGGTTGCCTGATTGACGTTAACGGCGTGCCTACGACCAATCCGGCGGTAATGCAGGAGTCGCCGTTGGGTTCGCTGTTGACCTTTGCCGAACATAAAGGCTACGCCCTTGCAGCGAtgt                                                           >  2:4070240/1‑150 (MQ=255)
                                                                                           tGGCATAAAGGCGTCCCCGTGCCGCCAGGTTGCCTGATTGACGTTAACGGCGTGCCTACGACCAATCCGGCGGTAATGCAGGAGTCGCCGTTGGGTTCGCTGTTGACCTTTGCCGAACATAAAGGCTACGCCCTTGCAGCGATGTGTGaa                                                      >  2:113349/1‑150 (MQ=255)
                                                                                           tGGCATAAAGGCGTCCCCGTGCCGCCAGGTTGCCTGATTGACGTTAACGGCGTGCCGACGACCAATCCGGCGGTAATGCAGGAGTCGCCGTTGGGTTCGCTGTTGACCTTTGCCGAACATAAAGGCTACGCCCTTGCAGCGATGTGTGaa                                                      >  1:5092859/1‑150 (MQ=255)
                                                                                            ggCATAAAGGCGTCCCCGTGCCGCCAGGTTGCCTGATTGACGTTAACGGCGTGCCGACGACCAATCCGGCGGTAATGCAGGAGTCGCCGTTGGGTTCGCTGTTGACCTTTGCCGAACATAAAGGCTACGCCCTTGCAGCGATGTGTGaaa                                                     <  2:253710/150‑1 (MQ=255)
                                                                                             gCATAAAGGCGTCCCCGTGCCGCCAGGTTGCCTGATTGACGTTAACGGCGTGCCTACGACCAATCCGGCGGTAATGCAGGAGTCGCCGTTGGGTTCGCTGTTGACCTTTGCCGAACATAAAGGCTACGCCCTTGCAGCGATGTGTGAAAt                                                    >  1:2756972/1‑150 (MQ=255)
                                                                                               aTAAAGGCGTCCCCGTGCCGCCAGGTTGCCTGATTGACGTTAACGGCGTGCCTACGACCAATCCGGCGGTAATGCAGGAGTCGCCGTTGGGTTCGCTGTTGACCTTTGCCGAACATAAAGGCTACGCCCTTGCAGCGATGTGTGAAATTc                                                  <  2:4882721/150‑1 (MQ=255)
                                                                                                  aaGGCGTCCCCGTGCCGCCAGGTTGCCTGATTGACGTTAACGGCGTGCCTACGACCAATCCGGCGGTAATGCAGGAGTCGCCGTTGGGTTCGCTGTTGACCTTTGCCGAACATAAAGGCTACGCCCTTGCAGCGATGTGTGAAATTCTTg                                               <  1:4892083/150‑1 (MQ=255)
                                                                                                  aaGGCGTCCCCGTGCCGCCAGGTTGCCTGATTGACGTTAACGGCGTGCCTACGACCAATCCGGCGGTAATGCAGGAGTCGCCGTTGGGTTCGCTGTTGACCTTTGCCGAACATAAAGGCTACGCCCTTGCAGCGATGTGTGAAATTCTTg                                               <  2:3849422/150‑1 (MQ=255)
                                                                                                    ggCGTCCCCGTGCCGCCAGGTTGCCTGATTGACGTTAACGGCGTGCCTACGACCAATCCGGCGGTAATGCAGGAGTCGCCGTTGGGTTCGCTGTTGACCTTTGCCGAACATAAAGGCTACGCCCTTGCAGCGATGTGTGAAATTCtt                                                <  2:4371506/147‑1 (MQ=255)
                                                                                                       gTCCCCGTGCCGCCAGGTTGCCTGATTGACGTTAACGGCGTGCCTACGACCAATCCGGCGGTAATGCAGGAGTCGCCGTTGGGTTCGCTGTTGACCTTTGCCGAACATAAAGGCTACGCCCTTGCAGCGATGTGTGAAATTCTTGGCggg                                          >  1:3083656/1‑150 (MQ=255)
                                                                                                          cccGTGCCGCCAGGTTGCCTGATTGACGTTAACGGCGTGCCTACGACCAATCCGGCGGTAATGCAGGAGTCGCCGTTGGGTTCGCTGTTGACCTTTGCCGAACATAAAGGCTACGCCCTTGCAGCGATGTGTGAAATTCTTGGCGGGgcg                                       <  1:4325650/150‑1 (MQ=255)
                                                                                                          cccGTGCCGCCAGGTTGCCTGATTGACGTTAACGGCGTGCCTACGACCAATCCGGCGGTAATGCAGGAGTCGCCGTTGGGTTCGCTGTTGACCTTTGCCGAACACAAAGGCTACGCCCTTGCAGCGATGTGTGAAATTCTTGGCGGGgcg                                       >  2:3085883/1‑150 (MQ=255)
                                                                                                          cccGTGCCGCCAGGTTGCCTGATTGACGTTAACGGCGTGCCGACGACCAATCCGGCGGTAATGCAGGAGTCGCCGTTGGGTTCGCTGTTGACCTTTGCCGAACATAAAGGCTACGCCCTTGCAGCGATGTGTGAAATTCTTGGCGGGgcg                                       <  1:1522997/150‑1 (MQ=255)
                                                                                                              tGCCGCCAGGTTGCCTGATTGACGTTAACGGCGTGCCTACGACCAATCCGGCGGTAATGCAGGAGTCGCCGTTGGGTTCGCTGTTGACCTTTGCCGAACATAAAGGCTACGCCCTTGCAGCGATGTGTGAAATTCTTGGCGGGGCGCttt                                   <  2:1720958/150‑1 (MQ=255)
                                                                                                              tGCCGCCAGGTTGCCTGATTGACGTTAACGGCGTGCCTACGACCAATCCGGCGGTAATGCAGGAGTCGCCGTTGGGTTCGCTGTTGACCTTTGCCGAACATAAAGGCTACGCCCTTGCAGCGATGTGTGAAATTCTTGGCGGGGCGCttt                                   <  2:3376471/150‑1 (MQ=255)
                                                                                                               gccgccAGGTTGCCTGATTGACGTTAACGGCGTGCCTACGACCAATCCGGCGGTAATGCAGGAGTCGCCGTTGGGTTCGCTGTTGACCTTTGCCGAACATAAAGGCTACGCCCTTGCAGCGATGTGTGAAATTCTTGGCGGGGCGCTTTc                                  >  2:1842015/1‑150 (MQ=255)
                                                                                                                     aGGTTGCCTGATTGACGTTAACGGCGTGCCGACGACCAATCCGGCGGTAATGCAGGAGTCGCCGTTGGGTTCGCTGTTGACCTTTGCCGAACATAAAGGCTACGCCCTTGCAGCGATGTGTGAAATTCTTGGCGGGGCGCTTTCcggcgg                            <  2:3072891/150‑1 (MQ=255)
                                                                                                                       gTTGCCTGATTGACGTTAACGGCGTGCCTACGACCAATCCGGCGGTAATGCAGGAGTCGCCGTTGGGTTCGCTGTTGACCTTTGCCGAACATAAAGGCTACGCCCTTGCAGCGATGTGTGACATTCTTGGCGGGGCGCTTTCCGGCGGTa                          >  2:2424501/1‑150 (MQ=255)
                                                                                                                         tGCCTGATTGACGTTAACGGCGTGCCTACGACCAATCCGGCGGTAATGCAGGAGTCGCCGTTGGGTTCGCTGTTGACCTTTGCCGAACATAAAGGCTACGCCCTTGCAGCGATGTGTGAAATTCTTGGCGGGGCGCTTTCCGGCGGTaaa                        >  2:2105612/1‑150 (MQ=255)
                                                                                                                           ccTGATTGACGTTAACGGCGTGCCTACGACCAATCCGGCGGTAATGCAGGAGTCGCCGTTGGGTTCGCTGTTGACCTTTGCCGAACATAAAGGCTACGCCCTTGCAGCGATGTGTGAAATTCTTGGCGGGGCGCTTTCCGGCGGTAAAac                      >  2:1607137/1‑150 (MQ=255)
                                                                                                                            cTGATTGACGTTAACGGCGTGCCTACGACCAATCCGGCGGTAATGCAGGAGTCGCCGTTGGGTTCGCTGTTGACCTTTGCCGAACATAAAGGCTACGCCCTTGCAGCGATGTGTGAAATTCTTGGCGGGGCGCTTTCCGGCGGTAAAacg                     >  1:2214567/1‑150 (MQ=255)
                                                                                                                              gATTGACGTTAACGGCGTGCCTACGACCAATCCGGCGGTAATGCAGGAGTCGCCGTTGGGTTCGCTGTTGACCTTTGCCGAACATAAAGGCTACGCCCTTGCAGCGATGTGTGAAATTCTTGGCGGGGCGCTTTCCGGCGGTAAAacgac                   <  1:4701834/150‑1 (MQ=255)
                                                                                                                               aTTGACGTTAACGGCGTGCCTACGACCAATCCGGCGGTAATGCAGGAGTCGCCGTTGGGTTCGCTGTTGACCTTTGCCGAACATAAAGGCTACGCCCTTGCAGCGATGTGTGAAATTCTTGGCGGGGCGCTTTCCGGCGGTAAAacgacg                  >  2:4982939/1‑150 (MQ=255)
                                                                                                                                   aCGTTAACGGCGTGCCTACGACCAATCCGGCGGTAATGCAGGAGTCGCCGTTGGGTTCGCTGTTGACCTTTGCCGAACATAAAGGCTACGCCCTTGCAGCGATGTGTGAAATTCTTGGCGGGGCGCTTTCCGGCGGTAAAACGACGCATc              <  1:2945687/150‑1 (MQ=255)
                                                                                                                                    cGTTAACGGCGTGCCTACGACCAATCCGGCGGTAATGCAGGAGTCGCCGTTGGGTTCGCTGTTGACCTTTGCCGAACATAAAGGCTACGCCCTTGCAGCGATGTGTGAAATTCTTGGCGGGGCGCTTTCCGGCGGTAAAACGACGCATCa             <  1:4532449/150‑1 (MQ=255)
                                                                                                                                        aaCGGCGTGCCTACGACCAATCCGGCGGTAATGCAGGAGTCGCCGTTGGGTTCGCTGTTGACCTTTGCCGAACATAAAGGCTACGCCCTTGCAGCGATGTGTGAAATTCTTGGCGGGGCGCTTTCCGGCGGTAAAACGACGCATCAGGaa         <  2:3014742/150‑1 (MQ=255)
                                                                                                                                          cGGCGTGCCTACGACCAATCCGGCGGTAATGCAGGAGTCGCCGTTGGGTTCGCTGTTGACCTTTGCCGAACATAAAGGCTACGCCCTTGCAGCGATGTGTGAAATTCTTGGCGGGGCGCTTTCCGGCGGTAAAACGACGCATCAGGAAAc       <  2:2316521/150‑1 (MQ=255)
                                                                                                                                          cGGCGTGCCTACGACCAATCCGGCGGTAATGCAGGAGTCGCCGTTGGGTTCGCTGTTGACCTTTGCCGAACATAAAGGCTACGCCCTTGCAGCGATGTGTGAAATTCTTGGCGGGGCGCTTTCCGGCGGTAAAACGACGCATCAGGAAAc       >  2:504945/1‑150 (MQ=255)
                                                                                                                                            gCGTGCCGACGACCAATCCGGCGGTAATGCAGGAGTCGCCGTTGGGTTCGCTGTTGACCTTTGCCGAACATAAAGGCTACGCCCTTGCAGCGATGTGTGAAATTCTTGGCGGGGCGCTTTCCGGCGGTAAAACGACGCATCAGGAAACGt     <  2:3399970/150‑1 (MQ=255)
                                                                                                                                            gCGTGCCGACGACCAATCCGGCGGTAATGCAGGAGTCGCCGTTGGGTTCGCTGTTGACCTTTGCCGAACATAAAGGCTACGCCCTTGCAGCGATGTGTGAAATTCTTGGCGGGGCGCTTTCCGGCGGTAAAACGACGCATCAGGAAACGt     <  2:822832/150‑1 (MQ=255)
                                                                                                                                              gTGCCTACGACCAATCCGGCGGTAATGCAGGAGTCGCCGTTGGGTTCGCTGTTGACCTTTGCCGAACATAAAGGCTACGCCCTTGCAGCGATGTGTGACATTCTTGGCGGGGCGCTTTCCGGCGGTAAAACGACGCATCAGGAAACGTTa   >  2:1836479/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                 
GTTCTGTGTGGTTTTCCCTCGTAAAGATAATTTCCCGCTGTTGCTTGATTACGCCACCAGCGCCATTGCATTTGGCAAAACCCGCGTCGCCTGGCATAAAGGCGTCCCCGTGCCGCCAGGTTGCCTGATTGACGTTAACGGCGTGCCGACGACCAATCCGGCGGTAATGCAGGAGTCGCCGTTGGGTTCGCTGTTGACCTTTGCCGAACATAAAGGCTACGCCCTTGCAGCGATGTGTGAAATTCTTGGCGGGGCGCTTTCCGGCGGTAAAACGACGCATCAGGAAACGTTAC  >  CP009273/832298‑832590

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑