Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 955,568 A→T 46.3% intergenic (+17/‑152) aroA → / → ycaL 5‑enolpyruvylshikimate‑3‑phosphate synthetase/putative peptidase‑related chaperone

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273955,5680AT46.3% 60.4 / inf 257intergenic (+17/‑152)aroA/ycaL5‑enolpyruvylshikimate‑3‑phosphate synthetase/putative peptidase‑related chaperone
Reads supporting (aligned to +/- strand):  ref base A (74/63);  new base T (47/72);  total (122/135)
Fisher's exact test for biased strand distribution p-value = 2.40e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.52e-01

Only 100 of 276 total aligned reads displayed.

TCACCGGATGGCGATGTGTTTCTCGCTGGTGGCGTTGTCAGATACACCAGTGACGATTCTTGATCCCAAATGCACGGCCAAAACATTTCCGGATTATTTCGAGCAGCTGGCGCGGATTAGCCAGGCAGCCTGAATGAACAACGGGCAATAAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGTTTTTTTTCGGACCTTGTGAGTCATTTTGATTAATGGTAGCGTCGCTTGTCAATGTAAGTTGTTGATACATAATATTTATATATGATTAATCAACGGATGATTC  >  CP009273/955419‑955717
                                                                                                                                                     |                                                                                                                                                     
 cACCGGATGGCGATGTGTTTCTCGCTGGTGGCGTTGTCAGATACACCAGTGACGATTCGTGATCCCAAATGCACGGCCAAAACAGTTCCGGATTATTTCGAGCAGCGGGCGCGGCTTAGCCCGGCAGCCTGAATGACCAACGGGCactaa                                                                                                                                                      >  2:4027475/1‑150 (MQ=255)
    cGGATGGCGATGTGTTTCTCGCTGGTGGCGTTGTCAGATACACCAGTGACGATTCTTGATCCCAAATGCACGGCCAAAACATTTCCGGATTATTTCGAGCAGCTGGCGCGGATTAGCCAGGCAGCCTGAATGAACAACGGGCaataaata                                                                                                                                                   <  2:85098/150‑1 (MQ=255)
     ggATGGCGATGTGTTTCTCGCTGGTGGCGTTGTCAGATACACCAGTGACGATTCTTGATCCCAAATGCACGGCCAAAACATTTCCGGATTATTTCGAGCAGCTGGCGCGGATTAGCCAGGCAGCCTGAATGAACAACGGGCAATAAATAg                                                                                                                                                  >  1:1986395/1‑150 (MQ=255)
       aTGGCGATGTGTTTCTCGCTGGTGGCGTTGTCAGATACACCAGTGACGATTCTTGATCCCAAATGCACGGCCAAAACATTTCCGGATTATTTCGAGCAGCTGGCGCGGATTAGCCAGGCAGCCTGAATGAACAACGGGCAATTAATAGcc                                                                                                                                                <  1:3653422/150‑1 (MQ=255)
            gATGTGTTTCTCGCTGGTGGCGTTGTCAGATACACCAGTGACGATTCTTGATCCCAAATGCACGGCCAAAACATTTCCGGATTATTTCGAGCAGCTGGCGCGGATTAGCCAGGCAGCCTGAATGAACAACGGGCAATAAATAGCCAAAtc                                                                                                                                           >  2:535061/1‑150 (MQ=255)
             aTGTGTTTCTCGCTGGTGGCGTTGTCAGATACACCAGTGACGATTCTTGATCCCAAATGCACGGCCAAAACATTTCCGGATTATTTCGAGCAGCTGGCGCGGATTAGCCAGGCAGCCTGAATGAACAACGGGCAATAAATAGCCAAAtct                                                                                                                                          <  2:115992/150‑1 (MQ=255)
                tgtTTCTCGCTGGTGGCGTTGTCAGATACACCAGTGACGATTCTTGATCCCAAATGCACGGCCAAAACATTTCCGGATTATTTCGAGCAGCTGGCGCGGATTAGCCAGGCAGCCTGAATGAACAACGGGCAATAAATAGCCAAAtctttc                                                                                                                                       >  2:2287308/1‑150 (MQ=255)
                  tttCTCGCTGGTGGCGTTGTCAGATACACCAGTGACGATTCTTGATCCCAAATGCACGGCCAAAACATTTCCGGATTATTTCGAGCAGCTGGCGCGGATTAGCCAGGCAGCCTGAATGAACAACGGGCAATTAATAGCCAAAtctttctt                                                                                                                                     <  1:2586709/150‑1 (MQ=255)
                   ttCTCGCTGGTGGCGTTGTCAGATACACCAGTGACGATTCTTGATCCCAAATGCACGGCCAAAACATTTCCGGATTATTTCGAGCAGCTGGCGCGGATTAGCCAGGCAGCCTGAATGAACAACGGGCAATTAATAGCCAAATctttcttt                                                                                                                                    <  1:5105234/150‑1 (MQ=255)
                    tctcGCTGGTGGCGTTGTCAGATACACCAGTGACGATTCTTGATCCCAAATGCACGGCCAAAACAGTTCCGGATTATTTCGAGCAGCTGGCGCGGATTAGCCAGGCAGCCTGAATGAACAACGGGCAATTAATAGCCAAATCTTTCTTTa                                                                                                                                   <  2:3765090/150‑1 (MQ=255)
                     ctcGCTGGTGGCGTTGTCAGATACACCAGTGACGATTCTTGATCCCAAATGCACGGCCAAAACATTTCCGGATTATTTCGAGCAGCTGGCGCGGATTAGCCAGGCAGCCTGAATGAACAACGGGCAATTAATAGCCAAATCTTTCTTTAt                                                                                                                                  >  2:747418/1‑150 (MQ=255)
                     ctcGCTGGTGGCGTTGTCAGATACACCAGTGACGATTCTTGATCCCAAATGCACGGCCAAAACATTTCCGGATTATTTCGAGCAGCTGGCGCGGATTAGCCAGGCAGCCTGAATGAACAACGGGCAATTAATAGCCAAATCTTTCTTTAt                                                                                                                                  >  2:481188/1‑150 (MQ=255)
                      tcGCTGGTGGCGTTGTCAGATACACCAGTGACGATTCTTGATCCCAAATGCACGGCCAAAACATTTCCGGATTATTTCGAGCAGCTGGCGCGGATTAGCCCGGCAGCCTGAATGAACAACGGGCAATAAATAGCCAAATCTTTCTTTATc                                                                                                                                 >  2:4154139/1‑150 (MQ=255)
                       cGCTGGTGGCGTTGTCAGATACACCAGTGACGATTCTTGATCCCAAATGCACGGCCAAAACATTTCCGGATTATTTCGAGCAGCTGGCGCGGATTAGCCAGGCAGCCTGAATGAACAACGGGCAATAAATAGCCAAATCTTTCTTTATCa                                                                                                                                >  2:1482088/1‑150 (MQ=255)
                         cTGGTGGCGTTGTCAGATACACCAGTGACGATTCTTGATCCCAACTGCACGGCCAAAACATTTCCGGATTATTTCGAGCAGCTGGCGCGGATTAGCCAGGCAGCCTGAATGAACAACGGGCAATAAATAGCCAAATCTTTCTTTATCaaa                                                                                                                              <  2:4726247/150‑1 (MQ=255)
                         cTGGTGGCGTTGTCAGATACACCAGTGACGATTCTTGATCCCAAATGCACGGCCAAAACATTTCCGGATTATTTCGAGCAGCTGGCGCGGATTAGCCAGGCAGCCTGAATGAACAACGGGCAATAAATAGCCAAATCTTTCTTTATCaaa                                                                                                                              <  1:1020672/150‑1 (MQ=255)
                          tggtggCGTTGTCAGATACACCAGTGACGATTCTTGATCCCAAATGCACGGCCAAAACATTTCCGGATTATTTCGAGCAGCTGGCGCGGATTAGCCAGGCAGCCTGAATGAACAACGGGCAATAAATAGCCAAATCTTTCTTTATCaaaa                                                                                                                             >  2:4306011/1‑150 (MQ=255)
                          tggtggCGTTGTCAGATACACCAGTGACGATTCTTGATCCCAAATGCACGGCCAAAACATTTCCGGATTATTTCGAGCAGCTGGCGCGGATTAGCCAGGCAGCCTGAATGAACAACGGGCAATAAATAGCCAAATCTTTCTTTATCaaaa                                                                                                                             <  1:503514/150‑1 (MQ=255)
                           ggtggCGTTGTCAGATACACCAGTGACGATTCTTGATCCCAAATGCACGGCCAAAACATTTCCGGATTATTTCGAGCAGCTGGCGCGGATTAGCCAGGCAGCCTGAATGAACAACGGGCAATAAATAGCCAAATCTTTCTTTATCAAAAc                                                                                                                            >  2:3250697/1‑150 (MQ=255)
                              ggCGTTGTCAGATACACCAGTGACGATTCTTGATCCCAAATGCACGGCCAAAACATTTCCGGATTATTTCGAGCAGCTGGCGCGGATTAGCCAGGCAGCCTGAATGAACAACGGGCAATAAATAGCCAAATCTTTCTTTATCAAAACGTc                                                                                                                         <  1:1504349/150‑1 (MQ=255)
                               gCGTTGTCAGATACACCAGTGACGATTCTTGATCCCAAATGCACGGCCAAAACATTTCCGGATTATTTCGAGCAGCTGGCGCGGATTAGCCAGGCAGCCTGAATGAACAACGGGCAATTAATAGCCAAATCTTTCTTTATCAAAACGTCg                                                                                                                        <  2:50694/150‑1 (MQ=255)
                                 gTTGTCAGATACACCAGTGACGATTCTTGATCCCAAATGCACGGCCAAAACATTTCCGGATTATTTCGAGCAGCTGGCGCGGATTAGCCAGGCAGCCTGAATGAACAACGGGCAATTAATAGCCAAATCTTTCTTTATCAAAACGTCGGc                                                                                                                      <  1:477809/150‑1 (MQ=255)
                                      cAGATACACCAGTGACGATTCTTGATCCCAAATGCACGGCCAAAACATTTCCGGATTATTTCGAGCAGCTGGCGCGGATTAGCCAGGCAGCCTGAATGAACAACGGGCAATTAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACAt                                                                                                                  <  2:446778/149‑1 (MQ=255)
                                      cAGATACACCAGTGACGATTCTTGATCCCAAATGCACGGCCAAAACATTTCCGGATTATTTCGAGCAGCTGGCGCGGATTAGCCAGGCAGCCTGAATGAACAACGGGCAATTAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACAt                                                                                                                  >  1:446778/1‑149 (MQ=255)
                                        gATACACCAGTGACGATTCTTGATCCCAAATGCACGGCCAAAACATTTCCGGATTATTTCGAGCAGCTGGCGCGGATTAGCCAGGCAGCCTGAATGAACAACGGGCAATAAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGt                                                                                                               >  2:723593/1‑150 (MQ=255)
                                        gATACACCAGTGACGATTCTTGATCCCAAATGCACGGCCAAAACATTTCCGGATTATTTCGAGCAGCTGGCGCGGATTAGCCAGGCAGCCTGAATGAACAACGGGCAATAAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGt                                                                                                               <  1:4965745/150‑1 (MQ=255)
                                          tACACCAGTGACGATTCTTGATCCCAAATGCACGGCCAAAACATTTCCGGATTATTTCGAGCAGCTGGCGCGGATTAGCCAGGCAGCCTGAATGAACAACGGGCAATAAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCg                                                                                                             <  2:2740513/150‑1 (MQ=255)
                                           acacCAGTGACGATTCTTGATCCCAAATGCACGGCCAAAACATTTCCGGATTATTTCGAGCAGCTGGCGCGGATTAGCCAGGCAGCCTGAATGAACAACGGGCAATAAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCgg                                                                                                            <  1:3467826/150‑1 (MQ=255)
                                            cacCAGTGACGATTCTTGATCCCAAATGCACGGCCAAAACATTTCCGGATTATTTCGAGCAGCTGGCGCGGATTAGCCAGGCAGCCTGAATGAACAACGGGCAATAAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGc                                                                                                           >  2:96879/1‑150 (MQ=255)
                                             acCAGTGACGATTCTTGATCCCAAATGCACGGCCAAAACATTTCCGGATTATTTCGAGCAGCTGGCGCGGATTAGCCAGGCAGCCTGAATGAACAACGGGCAATTAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCg                                                                                                          >  2:1662515/1‑150 (MQ=255)
                                              ccAGTGACGATTCTTGATCCCAAATGCACGGCCAAAACATTTCCGGATTATTTCGAGCAGCTGGCGCGGATTAGCCAGGCAGCCTGAATGAACAACGGGCAATAAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGt                                                                                                         <  1:1487356/150‑1 (MQ=255)
                                                aGTGACGATTCTTGATCCCAAATGCACGGCCAAAACATTTCCGGATTATTTCGAGCAGCTGGCGCGGATTAGCCAGGCAGCCTGAATGAACAACGGGCAATAAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGttt                                                                                                       >  2:2650668/1‑150 (MQ=255)
                                                  tGACGATTCTTGATCCCAAATGCACGGCCAAAACATTTCCGGATTATTTCGAGCAGCTGGCGCGGATTAGCCAGGCAGCCTGAATGAACAACGGGCAATAAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGttttt                                                                                                     <  2:770113/150‑1 (MQ=255)
                                                        ttCTTGATCCCAAATGCACGGCCAAAACATTTCCGGATTATTTCGAGCAGCTGGCGCGGATTAGCCAGGCAGCCTGAATGAACAACGGGCAATAAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGTTTTTTTTCgg                                                                                               <  2:1639081/150‑1 (MQ=255)
                                                         tCTTGATCCCAAATGCACGGCCAAAACATTTCCGGATTATTTCGAGCAGCTGGCGCGGATTAGCCCGGCAGCCTGAATGAACAACGGGCAATTAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGTTTTTTTTCGGa                                                                                              >  2:1219093/1‑150 (MQ=255)
                                                          cTTGATCCCAAATGCACGGCCAAAACATTTCCGGATTATTTCGAGCAGCTGGCGCGGATTAGCCAGGCAGCCTGAATGAACAACGGGCAATAAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGTTTTTTTTCTGAc                                                                                             >  2:3313783/1‑150 (MQ=255)
                                                            tGATCCCAAATGCACGGCCAAAACATTTCCGGATTATTTCGAGCAGCTGGCGCGGATTAGCCAGGCAGCCTGAATGAACAACGGGCAATTAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGTTTTTTTTCGGACCt                                                                                           >  1:4847468/1‑150 (MQ=255)
                                                             gATCCCAAATGCACGGCCAAAACATTTCCGGATTATTTCGAGCAGCTGGCGCGGATTAGCCAGGCAGCCTGAATGAACAACGGGCAATAAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGTTTTTTTTCGGACCtt                                                                                          >  2:584059/1‑150 (MQ=255)
                                                             gATCCCAAATGCACGGCCAAAACATTTCCGGATTATTTCGAGCAGCTGGCGCGGATTAGCCAGGCAGCCTGAATGAACAACGGGCAATAAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGTTTTTTTTCGGACCtt                                                                                          >  1:5138026/1‑150 (MQ=255)
                                                              aTCCCAAATGCACGGCCAAAACATTTCCGGATTATTTCGAGCAGCTGGCGCGGATTAGCCAGGCAGCCTGAATGAACAACGGGCAATAAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGTTTTTTTTCGGACCTtg                                                                                         <  1:3826827/150‑1 (MQ=255)
                                                              aTCCCAAATGCACGGCCAAAACATTTCCGGATTATTTCGAGCAGCTGGCGCGGATTAGCCAGGCAGCCTGAATGAACAACGGGCAATAAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGTTTTTTTTCGGACCTtg                                                                                         >  1:2310675/1‑150 (MQ=255)
                                                                    aaTGCACGGCCAAAACATTTCCGGATTATTTCGAGCAGCTGGCGCGGATTAGCCAGGCAGCCTGAATGAACAACGGGCAATAAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGTTTTTTTTCGGACCTTGTGAGTc                                                                                   <  1:749817/150‑1 (MQ=255)
                                                                     aTGCACGGCCAAAACATTTCCGGATTATTTCGAGCAGCTGGCGCGGATTAGCCAGGCAGCCTGAATGAACAACGGGCAATAAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGTTTTTTTTCGGACCTTGTGAGTCa                                                                                  <  2:2898292/150‑1 (MQ=255)
                                                                      tGCACGGCCAAAACATTTCCGGATTATTTCGAGCAGCTGGCGCGGATTAGCCAGGCAGCCTGAATGAACAACGGGCAATAAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGTTTTTTTTCGGACCTTGTGAGTCAt                                                                                 >  1:1829950/1‑150 (MQ=255)
                                                                           ggCCAAAACATTTCCGGATTATTTCGAGCAGCTGGCGCGGATTAGCCAGGCAGCCTGAATGAACAACGGGCAATTAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGTTTTTTTTCGGACCTTGTGAGTCATTTTGa                                                                            >  1:1115345/1‑150 (MQ=255)
                                                                           ggCCAAAACATTTCCGGATTATTTCGAGCAGCTGGCGCGGATTAGCCAGGCAGCCTGAATGAACAACGGGCAATTAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGTTTTTTTTCGGACCTTGTGAGTCATTTTGa                                                                            <  1:2987674/150‑1 (MQ=255)
                                                                           ggCCAAAACATTTCCGGATTATTTCGAGCAGCTGGCGCGGATTAGCCAGGCAGCCTGAATGAACAACGGGCAATAAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGTTTTTTTTCGGACCTTGTGAGTCATTTTGa                                                                            <  1:3945815/150‑1 (MQ=255)
                                                                              cAAAACATTTCCGGATTATTTCGAGCAGCTGGCGCGGATTAGCCAGGCAGCCTGAATGAACAACGGGCAATTAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGTTTTTTTTCGGACCTTGTGAGTCATTTTGATTa                                                                         <  2:1274389/150‑1 (MQ=255)
                                                                                aaaCATTTCCGGATTATTTCGAGCAGCTGGCGCGGATTAGCCAGGCAGCCTGAATGAACAACGGGCAATAAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACCTTGTCGGCGTTTTTTTTCGGACCTTGTGAGTCATTTTGATTAAt                                                                       >  2:1571757/1‑150 (MQ=255)
                                                                                 aaCATTTCCGGATTATTTCGAGCAGCTGGCGCGGATTAGCCAGGCAGCCTGAATGAACAACGGGCAATAAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGTTTTTTTTCGGACCTTGTGAGTCATTTTGATTAATg                                                                      <  1:4566048/150‑1 (MQ=255)
                                                                                   cATTTCCGGATTATTTCGAGCAGCTGGCGCGGATTAGCCAGGCAGCCTGAATGAACAACGGGCAATTAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGTTTTTTTTCGGACCTTGTGAGTCATTTTGATTAATGGt                                                                    <  1:3464073/150‑1 (MQ=255)
                                                                                   cATTTCCGGATTATTTCGAGCAGCTGGCGCGGATTAGCCAGGCAGCCTGAATGAACAACGGGCAATAAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGTTTTTTTTCGGACCTTTTGAGTCATTTTGATTAATGGt                                                                    >  1:1692699/1‑150 (MQ=255)
                                                                                   cATTTCCGGATTATTTCGAGCAGCTGGCGCGGATTAGCCAGGCAGCCTGAATGAACAACGGGCAATAAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGTTTTTTTTCGGACCTTGTGAGTCATTTTGATTAATGGt                                                                    >  2:4057519/1‑150 (MQ=255)
                                                                                    aTTTCCGGATTATTTCGAGCAGCTGGCGCGGATTAGCCAGGCAGCCTGAATGAACAACGGGCAATTAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGTTTTTTTTCGGACCTTGTGAGTCATTTTGATTAATGGTa                                                                   <  1:3534811/150‑1 (MQ=255)
                                                                                    aTTTCCGGATTATTTCGAGCAGCTGGCGCGGATTAGCCAGGCAGCCTGAATGAACAACGGGCAATAAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGTTTTTTTTCGGACCTTGTGAGTCATTTTGATTAATGGTa                                                                   >  1:4668066/1‑150 (MQ=255)
                                                                                      ttCCGGATTATTTCGAGCAGCTGGCGCGGATTAGCCAGGCAGCCTGAATGAACAACGGGCAATAAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGTTTTTTTTCGGACCTTGTGAGTCATTTTGATTAATGGTAGc                                                                 <  1:2183157/150‑1 (MQ=255)
                                                                                          ggATTATTTCGAGCAGCTGGCGCGGATTAGCCAGGCAGCCTGAATGAACAACGGGCAATTAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGTTTTTTTTCGGACCTTGTGAGTCATTTTGATTAATGGTAGCGTCg                                                             >  2:2823818/1‑150 (MQ=255)
                                                                                          ggATTATTTCGAGCAGCTGGCGCGGATTAGCCAGGCAGCCTGAATGAACAACGGGCAATAAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGTTTTTTTTCGGACCTTGTGAGTCATTTTGATTAATGGTAGCGTCg                                                             >  2:3934317/1‑150 (MQ=255)
                                                                                           gATTATTTCGAGCAGCTGGCGCGGATTAGCCAGGCAGCCTGAATGAACAACGGGCAATTAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGTTTTTTTTCGGACCTTGTGAGTCATTTTGATTAATGGTAGCGTCGc                                                            >  1:1551636/1‑150 (MQ=255)
                                                                                            attattTCGAGCAGCTGGCGCGGATTAGCCAGGCAGCCTGAATGAACAACGGGCAATAAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGTTTTTTTTCGGACCTTGTGAGTCATTTTGATTAATGGTAGCGTCGCt                                                           >  1:1295993/1‑150 (MQ=255)
                                                                                              tattTCGAGCAGCTGGCGCGGATTAGCCAGGCAGCCTGAATGAACAACGGGCAATAAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGTTTTTTTTCGGACCTTGTGAGTCATTTTGATTAATGGTAGCGTCGCTTg                                                         >  2:1831860/1‑150 (MQ=255)
                                                                                                tttCGAGCAGCTGGCGCGGATTAGCCAGGCAGCCTGAATGAACAACGGGCAATAAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGTTTTTTTTCGGACCTTGTGAGTCATTTTGATTAATGGTAGCGTCGCTTGTc                                                       >  1:2509578/1‑150 (MQ=255)
                                                                                                 ttCGAGCAGCTGGCGCGGATTAGCCAGGCAGCCTGAATGAACAACGGGCAATTAATAGCCAAATCTTTCTTTATCAAAACGTCGGCAAATTGTCGGCGTTTTTTTTCGGACCTTGTGAGTCATTTTGATTAATGGTAGCGTCGCTTGTCa                                                      <  1:1006438/150‑1 (MQ=255)
                                                                                                 ttCGAGCAGCTGGCGCGGATTAGCCAGGCAGCCTGAATGAACAACGGGCAATAAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGTTTTTTTTCGGACCTTGTGAGTCATTTTGATTAATGGTAGCGTCGCTTGTCa                                                      <  1:1839465/150‑1 (MQ=255)
                                                                                                  tCGAGCAGCTGGCGCGGATTAGCCAGGCAGCCTGAATGAACAACGGGCAATTAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGTTTTTTTTCGGACCTTGTGAGTCATTTTGATTAATGGTAGCGTCGCTTGTCaa                                                     >  2:4372591/1‑150 (MQ=255)
                                                                                                  tCGAGCAGCTGGCGCGGATTAGCCAGGCAGCCTGAATGAACAACGGGCAATTAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGTTTTTTTTCGGACCTTGTGAGTCATTTTGATTAATGGTAGCGTCGCTTGTCaa                                                     >  1:814053/1‑150 (MQ=255)
                                                                                                  tCGAGCAGCTGGCGCGGATTAGCCAGGCAGCCTGAATGAACAACGGGCAATAAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGTTTTTTTTCGGACCTTGTGAGTCATTTTGATTAATGGTAGCGTCGCTTGTCaa                                                     >  2:2800181/1‑150 (MQ=255)
                                                                                                   cGAGCAGCTGGCGCGGATTAGCCAGGCAGCCTGAATGAACAACGGGCAATTAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGTTTTTTTTCGGACCTTGTGAGTCATTTTGATTAATGGTAGCGTCGCGTGTCAAt                                                    >  1:861674/1‑150 (MQ=255)
                                                                                                     agcagcTGGCGCGGATTAGCCAGGCAGCCTGAATGAACAACGGGCAATTAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGTTTTTTTTCGGACCTTGTGAGTCATTTTGATTAATGGTAGCGTCGCTTGTCAATGt                                                  <  2:2342465/150‑1 (MQ=255)
                                                                                                       cagcTGGCGCGGATTAGCCAGGCAGCCTGAATGAACAACGGGCAATTAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGTTTTTTTTCGGACCTTGTGAGTCATTTTGATTAATGGTAGCGTCGCTTGTCAATGTaa                                                <  1:4223616/150‑1 (MQ=255)
                                                                                                         gcTGGCGCGGATTAGCCAGGCAGCCTGAATGAACAACGGGCAATTAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGTTTTTTTTCGGACCTTGTGAGTCATTTTGATTAATGGTAGCGTCGCTTGTCAATGTAAgt                                              <  2:4546858/150‑1 (MQ=255)
                                                                                                           tGGCGCGTATTCGCCAGGAAGCCTTAATCAACAACGGGAAATAAAAAGCCAAATCTTTCTTTATCAAAACGTCGGCAAATTGTCGGCGTTTTTTTTCGGACCTTGTGAGTCATTTTGATTAATGGTAGCGTCGCTTGTCAATGTAAgttg                                            <  1:4981341/150‑1 (MQ=255)
                                                                                                           tGGCGCGGATTAGCCAGGCAGCCTGAATGAACAACGGGCAATTAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGTTTTTTTTCGGACCTTGTGAGTCATTTTGATTAATGGTAGCGTCGCTTGTCAATGTAAgttg                                            <  2:3904479/150‑1 (MQ=255)
                                                                                                           tGGCGCGGATTAGCCAGGCAGCCTGAATGAACAACGGGCAATAAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGTTTTTTTTCGGACCTTGTGAGTCATTTTGATTAATGGTAGCGTCGCTTGTGAATGTAAgttg                                            >  1:1620725/1‑150 (MQ=255)
                                                                                                           tGGCGCGGATTAGCCAGGCAGCCTGAATGAACAACGGGCAATAAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGTTTTTTTTCGGACCTTGTGAGTCATTTTGATTAATGGTAGCGTCGCTTGTCAATGTAAgttg                                            >  2:3631659/1‑150 (MQ=255)
                                                                                                              cgcgGATTAGCCAGGCAGCCTGAATGAACAACGGGCAATAAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGTTTTTTTTCGGACCTTGTGAGTCATTTTGATTAATGGTAGCGTCGCTTGTCAATGTAAGttgttg                                         >  1:4613992/1‑150 (MQ=255)
                                                                                                              cgcgGATTAGCCAGGCAGCCTGAATGAACAACGGGCAATAAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGTTTTTTTTCGGACCTTGTGAGTCATTTTGATTAATGGTAGCGTCGCGTGTCAATGTAAGttgttg                                         >  1:4142545/1‑150 (MQ=255)
                                                                                                               gcgGATTAGCCAGGCAGCCTGAATGAACAACGGGCAATTAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGTTTTTTTTCGGACCTTGTGAGTCATTTTGATTAATGGTAGCGTCGCTTGTCAATGTAAGTTGTTGa                                        <  1:3266632/150‑1 (MQ=255)
                                                                                                               gcgGATTAGCCAGGCAGCCTGAATGAACAACGGGCAATAAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGTTTTTTTTCGGACCATGTGAGTCATTTTGATTAATGGTAGCGTCGCTTTTCAATGTTAgttttttt                                        >  1:897516/1‑148 (MQ=255)
                                                                                                                cgGATTAGCCAGGCAGCCTGAATGAACAACGGGCAATTAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGTTTTTTTTCGGACCTTGTGAGTCATTTTGATTAATGGTAGCGTCGCTTGTCAATTTAAGTTGTTGAt                                       >  2:452177/1‑150 (MQ=255)
                                                                                                                  gATTAGCCAGGCAGCCTGAATGAACAACGGGCAATTAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGTTTTTTTTCGGACCTTGTGAGTCATTTTGATTAATGGTAGCGTCGCTTGTCAATGTAAGTTGTTGATAc                                     <  2:4150689/150‑1 (MQ=255)
                                                                                                                       gCCAGGCAGCCTGAATGAACAACGGGCAATTAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGTTTTTTTTCGGACCTTGTGAGTCATTTTGATTAATGGTAGCGTCGCTTGTCAATGTAAGTTGTTGATACataat                                <  1:1183614/150‑1 (MQ=255)
                                                                                                                         cAGGCAGCCTGAATGAACAACGGGCAATTAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGTTTTTTTTCGGACCTTGTGAGTCATTTTGATTAATGGTAGCGTCGCTTGTCCATGTAAGTTGTTGATACATAatat                              >  2:1267856/1‑150 (MQ=255)
                                                                                                                           ggCAGCCTGAATGAACAACGGGCAATAAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGTTTTTTTTCGGACCTTGTGAGTCATTTTGATTAATGGTAGCGTCGCTTGTCAATGTAAGTTGTTGATACATAATAttt                            <  1:351786/150‑1 (MQ=255)
                                                                                                                            gCAGCCTGAATGAACAACGGGCAATTAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGTTTTTTTTCGGACCTTGTGAGTCATTTTGATTAATGGTAGCGTCGCTTGTCAATGTAAGTTGTTGATACATAATATTta                           <  2:1925541/150‑1 (MQ=255)
                                                                                                                            gCAGCCTGAATGAACAACGGGCAATTAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGTTTTTTTTCGGACCTTGTGAGTCATTTTGATTAATGGTAGCGTCGCTTGTCAATGTAAGTTGTTGATACATAAAATTta                           >  2:1453104/1‑150 (MQ=255)
                                                                                                                            gCAGCCTGAATGAACAACGGGCAATAAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGTTTTTTTTCGGACCTTGTGAGTCATTTTGATTAATGGTAGCGTCGCTTGTCAATGTAAGTTGTTGATACATAATATTta                           <  2:4675288/150‑1 (MQ=255)
                                                                                                                                 cTGAATGAACAACGGTCAATTAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGTTTTTTTTCGGACCTTGTGAGTCATTTTGATTAATGGTAGCGTCGCTTGTCAATGTAAGTTGTTGATACATAATATTTatatat                      <  2:3092459/150‑1 (MQ=255)
                                                                                                                                     atgaaCAACGGGCAATAAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGTTTTTTTTCGGACCTTGTGAGTCATTTTGATTAATGGTAGCGTCGCTTGTCAATGTAAGTTGTTGATACATAATATTTATATATGAtt                  <  2:4923333/150‑1 (MQ=255)
                                                                                                                                       gaaCAACGGGCAATTAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGTTTTTTTTCGGACCTTGTGAGTCATTTTGATTAATGGTAGCGTCGCTTGTCAATGTAAGTTGTTGATACATAATATTTATATATGATTaa                <  2:1286749/150‑1 (MQ=255)
                                                                                                                                        aacaacGGGCAATTAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGTTTTTTTTCGGACCTTGTGAGTCATTTTGATTAATGGTAGCGTCGCTTGTCAATGTAAGTTGTTGATACATAATATTTATATATGATTAAt               <  2:4289005/150‑1 (MQ=255)
                                                                                                                                        aacaacGGGCAATAAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGTTTTTTTTCGGACCGTGTGAGTCATTTTGATTAATGGTAGCGTCGCGTGTCAATGTTAGTTGTTGGTGAAGAATATTTATATATGATTAAt               >  2:4753762/1‑150 (MQ=255)
                                                                                                                                          caacGGGCAATAAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGTTTTTTTTCGGACCTTGTGAGTCATTTTGATTAATGGTAGCGTCGCGTGTCAAGGTAAGTTGTTGATACATAATATTTATATATTATTaaaaa             >  1:2075895/1‑147 (MQ=255)
                                                                                                                                            acGGGCAATTAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGTTTTTTTTCGGACCTTGTGAGTCATTTTGATTAATGGTAGCGTCGCTTGTCAATGTAAGTTGTTGATACATAATATTTATATATGATTAATCAAc           <  1:556051/150‑1 (MQ=255)
                                                                                                                                              gggCAATTAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGTTTTTTTTCGGACCTTGTGAGTCATTTTGATTAATGGTAGCGTCGCTTGTCAATGTAAGTTGTTGATACATAATATTTATATATGATTAATCAACgg         <  1:4069797/150‑1 (MQ=255)
                                                                                                                                              gggCAATAAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGTTTTTTTTCGGACCTTGTGAGTCATTTTGATTAATGGTAGCGTCGCGGGTGAAAGTAAGTTGTTGATACATAATATTTATATATGATTAATAAACgg         >  1:3189858/1‑150 (MQ=255)
                                                                                                                                                gCAATAAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGTTTTTTTTCGGACCTTGTGAGTCATTTTGATTAATGGTAGCGTCGCTTGTCAATGTAAGTTGTTGATACATAATATTTATATATGATTAATCAACGgat       <  1:2747114/150‑1 (MQ=255)
                                                                                                                                                 cAATAAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGTTTTTTTTCGGACCTTGTGAGTCATTTTGATTAATGGTAGCGTCGCTTGTCAATGTAAGTTGTTGATACATAATATTTATATATGATTAATCAACGgatg      <  1:2826900/150‑1 (MQ=255)
                                                                                                                                                   ataaataGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGTTTTTTTTCGGACCTTGTGAGTCATTTTGATTAATGGTAGCGTCGCTTGTCAATGTAAGTTGTTGATACATAATATTTATATATGATTAATCAACGgatgat    >  2:2080351/1‑150 (MQ=255)
                                                                                                                                                     aaataGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGTTTTTTTTCGGACCTTGTGAGTCATTTTGATTAATGGTAGCGTCGCTTGTCAATGTAAGTTGTTGATACATAATATTTATATATGATTAATCAACGGATGttta  >  2:3125290/1‑149 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
TCACCGGATGGCGATGTGTTTCTCGCTGGTGGCGTTGTCAGATACACCAGTGACGATTCTTGATCCCAAATGCACGGCCAAAACATTTCCGGATTATTTCGAGCAGCTGGCGCGGATTAGCCAGGCAGCCTGAATGAACAACGGGCAATAAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGTTTTTTTTCGGACCTTGTGAGTCATTTTGATTAATGGTAGCGTCGCTTGTCAATGTAAGTTGTTGATACATAATATTTATATATGATTAATCAACGGATGATTC  >  CP009273/955419‑955717

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑