BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	1,122,582	G→T	6.3%	V107V (GTG→GTT)	yceM →	putative oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	1,122,582	0	G	T	6.3%	1199.0 / 35.8	303	V107V (GTG→GTT)	yceM	putative oxidoreductase
Reads supporting (aligned to +/- strand): ref base G (129/155); new base T (10/9); total (139/164)
Fisher's exact test for biased strand distribution p-value = 6.37e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.84e-01

Only 100 of 310 total aligned reads displayed.

TCCAGCCTTGCCGCCAGTTGCGATGCGGTTTTTGTGCATTCCAGCACCGCCAGCCACTTTGACGTGGTCAGTACGTTACTCAATGCGGGGGTACATGTCTGTGTCGATAAACCGCTGGCAGAAAATCTGCGCGATGCTGAACGGCTGGTGGAACTGGCGGCGCGGAAAAAACTGACGTTGATGGTCGGTTTTAACCGTCGTTTCGCACCACTCTACGGTGAGTTAAAAACGCAACTCGCCACCGCAGCCTCGCTAAGAATGGATAAACATCGTAGCAATAGCGTCGGGCCACACGATC  >  CP009273/1122433‑1122730

  cAGCCTTGCCGCCAGTTGCGATGCGGTTTTTGTGCATTCCAGCACCGCCAGCCACTTTGACGTGGTCAGTACGTTACTCAATGCGGGGGTACATGTCTGTGTCGATAAACCGCTGGCAGAAAATCTGCGCGATGCTGAACGGCTGGTTGa                                                                                                                                                    >  2:2853147/1‑150 (MQ=255)

  cAGCCTTGCCGCCAGTTGCGATGCGGTTTTTGTGCATTCCAGCACCGCCAGCCACTTTGACGTGGTCAGTACGTTACTCAATGCGGGGGTACATGTCTGTGTCGATAAACCGCTGGCAGAAAATCTGCGCGATGCTGAACGGCTGGTGGa                                                                                                                                                    <  1:4768100/150‑1 (MQ=255)

  cAGCCTTGCCGCCAGTTGCGATGCGGTTTTTGTGCATTCCAGCACCGCCAGCCACTTTGACGTGGTCAGTACGTTACTCAATGCGGGGGTACATGTCTGTGTCGATAAACCGCTGGCAGAAAATCTGCGCGATGCTGAACGGCTGGTGGa                                                                                                                                                    >  1:5146011/1‑150 (MQ=255)

  cAGCCTTGCCGCCAGTTGCGATGCGGTTTTTGTGCATTCCAGCACCGCCAGCCACTTTGACGTGGTCAGTACGTTACTCAATGCGGGGGTACATGTCTGTGTCGATAAACCGCTGGCAGAAAATCTGCGCGATGCTGAACGGCTGGTGGa                                                                                                                                                    <  1:163298/150‑1 (MQ=255)

     ccTTGCCGCCAGTTGCGATGCGGTTTTTGTGCATTCCAGCACCGCCAGCCACTTTGACGTGGTCAGTACGTTACTCAATGCGGGGGTACATGTCTGTGTCGATAAACCGCTGGCAGAAAATCTGCGCGATGCTGAACGGCTGGTGGAACt                                                                                                                                                 <  2:2116475/150‑1 (MQ=255)

     ccTTGCCGCCAGTTGCGATGCGGTTTTTGTGCATTCCAGCACCGCCAGCCACTTTGACGTGGTCAGTACGTTACTCAATGCGGGGGTACATGTCTGTGTCGATAAACCGCTGGCAGAAAATCTGCGCGATGCTGAACGGCTGGTGGAACt                                                                                                                                                 <  1:4287478/150‑1 (MQ=255)

      cTTGCCGCCAGTTGCGATGCGGTTTTTGTGCATTCCAGCACCGCCAGCCACTTTGACGTGGTCAGTACGTTACTCAATGCGGGGGTACATGTCTGTGTCGATAAACCGCTGGCAGAAAATCTGCGCGATGCTGAACGGCTGGTGGAACTg                                                                                                                                                >  2:153793/1‑150 (MQ=255)

      attGCCGCCAGTTGCGATGCGGTTTTTGTGCATTCCAGCACCGCCAGCCACTTTGACGTGGTCAGTACGTTACTCAATGCGGGGGTACATGTCTGTGTCGATAAACCGATGGCAGAAAATCTGCGCGATGCTGAACGGCTGGTGGACCTg                                                                                                                                                >  1:1310989/2‑150 (MQ=255)

       ttGCCGCCAGTTGCGATGCGGTTTTTGTGCATTCCAGCACCGCCAGCCACTTTGACGTGGTCAGTACGTTACTCAATGCGGGGGTACATGTCTGTGTCGATAAACCGCTGGCAGAAAATCTGCGCGATGCTGAACGGCTGGTGGAACTgg                                                                                                                                               >  1:2723192/1‑150 (MQ=255)

        tGCCGCCAGTTGCGATGCGGTTTTTGTGCATTCCAGCACCGCCAGCCACTTTGACGTGGTCAGTACGTTACTCAATGCGGGGGTACATGTCTGTGTCGATAAACCGCTGGCAGAAAATCTGCGCGATGCTGAACGGCTGGTGGAACTggc                                                                                                                                              <  2:671219/150‑1 (MQ=255)

        tGCCGCCAGTTGCGATGCGGTTTTTGTGCATTCCAGCACCGCCAGCCACTTTGACGTGGTCAGTACGTTACTCAATGCGGGGGTACATGTCTGTGTCGATAAACCGCTGGCAGAAAATCTGCGCGATGCTGAACGGCTGGTGGAACTggc                                                                                                                                              <  1:1981911/150‑1 (MQ=255)

         gccgccAGTTGCGATGCGGTTTTTGTGCATTCCAGCACCGCCAGCCACTTTGACGTGGTCAGTACGTTACTCAATGCGGGGGTACATGTCTGTGTCGATAAACCGCTGGCAGAAAATCTGCGCGATGCTGAACGGCTGGTGGAACTggcg                                                                                                                                             >  2:2002065/1‑150 (MQ=255)

           cgccAGTTGCGATGCGGTTTTTGTGCATTCCAGCACCGCCAGCCACTTTGACGTGGTCAGTACGTTACTCAATGCGGGGGTACATGTCTGTGTCGATAAACCGCTGGCAGAAAATCTGCGCGATGCTGAACGGCTGGTGGAACTggcggc                                                                                                                                           <  2:2993272/150‑1 (MQ=255)

                  tGCGATGCGGTTTTTGTGCATTCCAGCACCGCCAGCCACTTTGACGTGGTCAGTACGTTACTCAATGCGGGGGTACATGTCTGTGTCGATAAACCGCTGGCAGAAAATCTGCGCGATGCTGAACGGCTGGTGGAACTGGCGGCGCGGaaa                                                                                                                                    <  2:1718906/150‑1 (MQ=255)

                  tGCGATGCGGTTTTTGTGCATTCCAGCACCGCCAGCCACTTTGACGTGGTCAGTACGTTACTCAATGCGGGGGTACATGTCTGTGTCGATAAACCGCTGGCAGAAAATCTGCGCGATGCTGAACGGCTGGTGGAACTGGCGGCGCGGaaa                                                                                                                                    >  1:3479032/1‑150 (MQ=255)

                    cGATGCGGTTTTTGTGCATTCCAGCACCGCCAGCCACTTTGACGTGGTCAGTACGTTACTCAATGCGGGGGTACATGTCTGTGTCGATAAACCGCTGGCAGAAAATCTGCGCGATGCTGAACGGCTGGTGGAACTGGCGGCGCGGaaaaa                                                                                                                                  >  1:2049912/1‑150 (MQ=255)

                     gATGCGGTTTTTGTGCATTCCAGCACCGCCAGCCACTTTGACGTGGTCAGTACGTTACTCAATGCGGGGGTACATGTCTGTGTCGATAAACCGCTGGCAGAAAATCTGCGCGATGCTGAACGGCTGGTGGAACTGGCGGCGCGGaaaaaa                                                                                                                                 <  2:1209274/150‑1 (MQ=255)

                     gATGCGGTTTTTGTGCATTCCAGCACCGCCAGCCACTTTGACGTGGTCAGTACGTTACTCAATGCGGGGGTACATGTCTGTGTCGATAAACCGCTGGCAGAAAATCTGCGCGATGCTGAACGGCTGGTGGAACTGGCGGCGCGGaaaaaa                                                                                                                                 >  1:1533840/1‑150 (MQ=255)

                       tGCGGTTTTTGTGCATTCCAGCACCGCCAGCCACTTTGACGTGGTCAGTACGTTACTCAATGCGGGGGTACATGTCTGTGTCGATAAACCGCTGGCAGAAAATCTGCGCGATGCTGAACGGCTGGTGGAACTGGCGGCGCGGAAAAAACt                                                                                                                               <  1:2292591/150‑1 (MQ=255)

                          ggTTTTTGCGCATTCCAGCCCCGCCAGCCACTTTGACGTGGTCAGTACGTTACTCAAAGCGGGGGTACATGTCGGGGTCGATAAACCGCTGGCAGAAAATCTGCGCGATGCTGACCGGCTGGTGGAACTGGCGGCGCGGAAAAAACTGAc                                                                                                                            <  1:119108/150‑1 (MQ=255)

                           gTTTTTGTGCATTCCAGCACCGCCAGCCACTTTGACGTGGTCAGTACGTTACTCAATGCGGGGGTACATGTCTGTGTCGATAAACCGCTGGCAGAAAATCTGCGCGATGCTGAACGGCTGGTGGAACTGGCGGCGCGGAAAAAACTGACg                                                                                                                           <  1:1318811/150‑1 (MQ=255)

                           gTTTTTGTGCATTCCAGCACCGCCAGCCACTTTGACGTGGTCAGTACGTTACTCAATGCGGGGGTACATGTCTGTGTCGATAAACCGCTGGCAGAAAATCTGCGCGATGCTGAACGGCTGGTGGAACTGGCGGCGCGGAAAAAACTGACg                                                                                                                           <  2:276852/150‑1 (MQ=255)

                              tttGTGCATTCCAGCACCGCCAGCCACTTTGACGTGGTCAGTACGTTACTCAATGCGGGGGTACATGTCTGTGTCGATAAACCGCTGGCAGAAAATCTGCGCGATGCTGAACGGCTGGTTGAACTGGCGGCGCGGAAAAAACTGACGTTg                                                                                                                        >  1:2236602/1‑150 (MQ=255)

                                     aTTCCAGCACCGCCAGCCACTTTGACGTGGTCAGTACGTTACTCAATGCGGGGGTACATGTCTGTGTCGATAAACCGCTGGCAGAAAATCTGCGCGATGCTGAACGGCTGGTGGAACTGGCGGCGCGGAAAAAACTGACGTTGATGGTCg                                                                                                                 <  2:4331904/150‑1 (MQ=255)

                                     aTTCCAGCACCGCCAGCCACTTTGACGTGGTCAGTACGTTACTCAATGCGGGGGTACATGTCTGTGTCGATAAACCGCTGGCAGAAAATCTGCGCGATGCTGAACGGCTGGTGGAACTGGCGGCGCGGAAAAAACTGACGTTGATGGTCg                                                                                                                 >  2:1778827/1‑150 (MQ=255)

                                       tCCAGCACCGCCAGCCACTTTGACGTGGTCAGTACGTTACTCAATGCGGGGGTACATGTCTGTGTCGATAAACCGCTGGCAGAAAATCTGCGCGATGCTGAACGGCTGGTGGAACTGGCGGCGCGGAAAAAACTGACGTTGATGGTCGGt                                                                                                               <  1:2481737/150‑1 (MQ=255)

                                          aGCACCGCCAGCCACTTTGACGTGGTCAGTACGTTACTCAATGCGGGGGTACATGTCTGTGTCGATAAACCGCTGGCAGAAAATCTGCGCGATGCTGAACGGCTGGTGGAACTGGCGGCGCGGAAAAAACTGACGTTGATGGTCGGtttt                                                                                                            >  2:4462455/1‑150 (MQ=255)

                                          aGCACCGCCAGCCACTTTGACGTGGTCAGTACGTTACTCAATGCGGGGGTACATGTCTGTGTCGATAAACCGCTGGCAGAAAATCTGCGCGATGCTGAACGGCTGGTGGAACTGGCGGCGCGGAAAAAACTGACGTTGATGGTCGGtttt                                                                                                            >  2:4370212/1‑150 (MQ=255)

                                          aGCACCGCCAGCCACTTTGACGTGGTCAGTACGTTACTCAATGCGGGGGTACATGTCTGTGTCGATAAACCGCTGGCAGAAAATCTGCGCGATGCTGAACGGCTGGTGGAACTGGCGGCGCGGAAAAAACTGACGTTGATGGTCGGtttt                                                                                                            <  2:1320804/150‑1 (MQ=255)

                                                gccagccaCTTTGACGTGGTCAGTACGTTACTCAATGCGGGGGTACATGTCTGTGTCGATAAACCGCTGGCAGAAAATCTGCGCGATGCTGAACGGCTGGTGGAACTGGCGGCGCGGAAAAAACTGACGTTGATGGTCGGTTTTAACcgt                                                                                                      >  2:197561/1‑150 (MQ=255)

                                                  cagccaCTTTGACGTGGTCAGTACGTTACTCAATGCGGGGGTACATGTCTGTGTCGATAAACCGCTGGCAGAAAATCTGCGCGATGCTGAACGGCTGGTGGAACTGGCGGCGCGGAAAAAACTGACGTTGATGGTCGGTTTTAACcgtcg                                                                                                    <  1:4909162/150‑1 (MQ=255)

                                                     ccaCTTTGACGTGGTCAGTACGTTACTCAATGCGGGGGTACATGTCTGTGTCGATAAACCGCTGGCAGAAAATCTGCGCGATGCTGAACGGCTGGTGGAACTGGCGGCGCGGAAAAAACTGACGTTGATGGTCGGTTTTAACCGTCGttt                                                                                                 <  2:26343/150‑1 (MQ=255)

                                                      caCTTTGACGTGGTCAGTACGTTACTCAATGCGGGGGTACATGTCTGTGTCGATAAACCGCTGGCAGAAAATCTGCGCGATGCTGAACGGCTGGTGGAACTGGCGGCGCGGAAAAAACTGACGTTGATGGTCGGTTTTAACCGTCGTTTc                                                                                                <  1:3329948/150‑1 (MQ=255)

                                                      caCTTTGACGTGGTCAGTACGTTACTCAATGCGGGGGTACATGTCTGTGTCGATAAACCGCTGGCAGAAAATCTGCGCGATGCTGAACGGCTGGTGGAACTGGCGGCGCGGAAAAAACTGACGTTGATGGTCGGTTTTAACCGTCGTTTc                                                                                                >  2:1159989/1‑150 (MQ=255)

                                                        cTTTGACGTGGTCAGTACGTTACTCAATGCGGGGGTACATGTCTGTGTCGATAAACCGCTGGCAGAAAATCTGCGCGATGCTGAACGGCTGGTGGAACTGGCGGCGCGGAAAAAACTGACGTTGATGGTCGGTTTTAACCGTCGTTTCGc                                                                                              >  2:3438083/1‑150 (MQ=255)

                                                        cTTTGACGTGGTCAGTACGTTACTCAATGCGGGGGTACATGTCTGTGTCGATAAACCGCTGGCAGAAAATCTGCGCGATGCTGAACGGCTGGTGGAACTGGCGGCGCGGAAAAAACTGACGTTGATGGTCGGTTTTAACCGTCGTTTCGc                                                                                              >  2:1468340/1‑150 (MQ=255)

                                                              cGTGGTCAGTACGTTACTCAATGCGGGGGTACATGTCTGTGTCGATAAACCGCTGGCAGAAAATCTGCGCGATGCTGAACGGCTGGTGGAACTGGCGGCGCGGAAAAAACTGACGTTGATGGTCGGTTTTAACCGTCGTTTCGCACCAct                                                                                        >  2:2571578/1‑150 (MQ=255)

                                                                 ggTCAGTACGTTACTCAATGCGGGGGTACATGTCTGTGTCGATAAACCGCTGGCAGAAAATCTGCGCGATGCTGAACGGCTGGTTGAACTGGCGGCGCGGAAAAAACTGACGTTGATGGTCGGTTTTAACCGTCGTTTCGCACCACTCTa                                                                                     <  2:2662503/150‑1 (MQ=255)

                                                                  gTCAGTACGTTACTCAATGCGGGGGTACATGTCTGTGTCGATAAACCGCTGGCAGAAAATCTGCGCGATGCTGAACGGCTGGTGGAACTGGCGGCGCGGAAAAAACTGACGTTGATGGTCGGTTTTAACCGTCGTTTCGCACCACTCTAc                                                                                    >  1:4396719/1‑150 (MQ=255)

                                                                  gTCAGTACGTTACTCAATGCGGGGGTACATGTCTGTGTCGATAAACCGCTGGCAGAAAATCTGCGCGATGCTGAACGGCTGGTGGAACTGGCGGCGCGGAAAAAACTGACGTTGATGGTCGGTTTTAACCGTCGTTTCGCACCACTCTAc                                                                                    <  2:4924006/150‑1 (MQ=255)

                                                                  gTCAGTACGTTACTCAATGCGGGGGTACATGTCTGTGTCGATAAACCGCTGGCAGAAAATCTGCGCGATGCTGAACGGCTGGTGGAACAGGCGGCGCGGAAAAAACTGACGTTGATGGTCGGTTTTAACCGTCGTTTCGCACCACTCTAc                                                                                    >  1:449606/1‑150 (MQ=255)

                                                                    cAGTTCGTTACTCAATGCGGGGGTACATGTCTGTGTCGATAAACCGCTGGCAGAAAATCTGCGCGATGCTGAACGGCTGGTGGAACTGGCGGCGCGGAAAAAACTGACGTTGATGGTCGGTTTTAACCGTCGTTTCGCACCACTCTACgg                                                                                  <  1:3042080/150‑1 (MQ=255)

                                                                    cAGTACGTTACTCAATGCGGGGGTACATGTCTGTGTCGATAAACCGCTGGCAGAAAATCTGCGCGATGCTGAACGGCTGGTGGAACTGGCGGCGCGGAAAAAACTGACGTTGATGGTCGGTTTTAACCGTCGTTTCGCACCACTCTACgg                                                                                  >  1:2433678/1‑150 (MQ=255)

                                                                      gTACGTTACTCAATGCGGGGGTACATGTCTGTGTCGATAAACCGCTGGCAGAAAATCTGCGCGATGCTGAACGGCTGGTGGAACTGGCGGCGCGGAAAAAACTGACGTTGATGGTCGGTTTTAACCGTCGTTTCGCACCACTCTACGGTg                                                                                <  2:3817708/150‑1 (MQ=255)

                                                                      gTACGTTACTCAATGCGGGGGTACATGTCTGTGTCGATAAACCGCTGGCAGAAAATCTGCGCGATGCTGAACGGCTGGTGGAACTGGCGGCGCGGAAAAAACTGACGTTGATGGTCGGTTTTAACCGTCGTTTCGCACCACTCTACGGTg                                                                                <  1:1250728/150‑1 (MQ=255)

                                                                        aCGTTACTCAATGCGGGGGTACATGTCTGTGTCGATAAACCGCTGGCAGAAAATCTGCGCGATGCTGAACGGCTGGTTGAACTGGCGGCGCGGAAAAAACTGACGTTGATGGTCGGTTTTAACCGTCGTTTCGCACCACTCTACGGTGAg                                                                              <  1:2853147/150‑1 (MQ=255)

                                                                        aCGTTACTCAATGCGGGGGTACATGTCTGTGTCGATAAACCGCTGGCAGAAAATCTGCGCGATGCTGAACGGCTGGTGGAACTGGCGGCGCGGAAAAAACTGACGTTGATGGTCGGTTTTAACCGTCGTTTCGCACCACTCTACGGTGAg                                                                              >  1:1960499/1‑150 (MQ=255)

                                                                          gTTACTCAATGCGGGGGTACATGTCTGTGTCGATAAACCGCTGGCAGAAAATCTGCGCGATGCTGAACGGCTGGTGGAACTGGCGGCGCGGAAAAAACTGACGTTGATGGTCGGTTTTAACCGTCGTTTCGCACCACTCTACGGTGAGtt                                                                            <  1:3983218/150‑1 (MQ=255)

                                                                          gTTACTCAATGCGGGGGTACATGTCTGTGTCGATAAACCGCTGGCAGAAAATCTGCGCGATGCTGAACGGCTGGTGGAACTGGCGGCGCGGAAAAAACTGACGTTGATGGTCGGTTTTAACCGTCGTTTCGCACCACTCTACGGTGAGtt                                                                            >  2:3651082/1‑150 (MQ=255)

                                                                                cAATGCGGGGGTACATGTCTGTGTCGATAAACCGCTGGCAGAAAATCTGCGCGATGCTGAACGGCTGGTTGAACTGGCGGCGCGGAAAAAACTGACGTTGATGGTCGGTTTTAACCGTCGTTTCGCACCACTCTACGGTGAGTTAAAAAc                                                                      >  1:4744157/1‑150 (MQ=255)

                                                                                cAATGCGGGGGTACATGTCTGTGTCGATAAACCGCTGGCAGAAAATCTGCGCGATGCTGAACGGCTGGTGGAACTGGCGGCGCGGAAAAAACTGACGTTGATGGTCGGTTTTAACCGTCGTTTCGCACCACTCTACGGTGAGTTAAAAAc                                                                      <  2:3393772/150‑1 (MQ=255)

                                                                                cAATGCGGGGGTACATGTCTGTGTCGATAAACCGCTGGCAGAAAATCTGCGCGATGCTGAACGGCTGGTGGAACTGGCGGCGCGGAAAAAACTGACGTTGATGGTCGGTTTTAACCGTCGTTTCGCACCACTCTACGGTGAGTTAAAAAc                                                                      <  1:101288/150‑1 (MQ=255)

                                                                                      gggggTACATGTCTGTGTCGATAAACCGCTGGCAGAAAATCTGCGCGATGCTGAACGGCTGGTGGACCTGGCGGCGCGGAAAAAACTGACGTTGATGGTCGGTTTTAACCGTCGTTTCGCACCACTCTACGGTGAGTTAAAAACGCAACt                                                                >  2:4688345/1‑150 (MQ=255)

                                                                                      gggggTACATGTCTGTGTCGATAAACCGCTGGCAGAAAATCTGCGCGATGCTGAACGGCTGGTGGAACTGGCGGCGCGGAAAAAACTGACGTTGATGGTCGGTTTTAACCGTCGTTTCGCACCACTCTACGGTGAGTTAAAAACGCAACt                                                                >  1:727106/1‑150 (MQ=255)

                                                                                      gggggTACATGTCTGTGTCGATAAACCGCTGGCAGAAAATCTGCGCGATGCTGAACGGCTGGTGGAACTGGCGGCGCGGAAAAAACTGACGTTGATGGTCGGTTTTAACCGTCGTTTCGCACCACTCTACGGTGAGTTAAAAACGCAACt                                                                <  1:4551505/150‑1 (MQ=255)

                                                                                      gggggTACATGTCTGTGTCGATAAACCGCTGGCAGAAAATCTGCGCGATGCTGAACGGCTGGTGGAACTGGCGGCGCGGAAAAAACTGACGTTGATGGTCGGTTTTAACCGTCGTTTCGCACCACTCTACGGTGAGTTAAAAACGCAACt                                                                >  2:417407/1‑150 (MQ=255)

                                                                                        gggTACATGTCTGTGTCGATAAACCGCTGGCAGAAAATCTGCGCGATGCTGAACGGCTGGTGGAACTGGCGGCGCGGAAAAAACTGACGTTGATGGTCGGTTTTAACCGTCGTTTCGCACCACTCTACGGTGAGTTAAAAACGCAACTCg                                                              <  1:142565/150‑1 (MQ=255)

                                                                                        gggTACATGTCTGTGTCGATAAACCGCTGGCAGAAAATCTGCGCGATGCTGAACGGCTGGTGGAACTGGCGGCGCGGAAAAAACTGACGTTGATGGTCGGTTTTAACCGTCGTTTCGCACCACTCTACGGTGAGTTAAAAACGCAACTCg                                                              <  1:3638527/150‑1 (MQ=255)

                                                                                              ttGTCTGTGTCGATAAACCGCTGGCAGAAAATCTGCGCGTTGCTGAACGGCTGGTGGAACTGTCGGCGCGGAAAAAACTGACGTTGATGGTCGGTTTTAACCGTCGTTTCGCACCACTCTACGGTGAGTTAAAAACGCAACTCGCCACCg                                                        <  1:178267/149‑1 (MQ=255)

                                                                                                gTCTGTGTCGATAAACCGCTGGCAGAAAATCTGCGCGATGCTGAACGGCTGGTGGAACTGGCGGCGCGGAAAAAACTGACGTTGATGGTCGGTTTTAACCGTCGTTTCGCACCACTCTACGGTGAGTTAAAAACGCAACTCGCCACCGCa                                                      >  1:1837932/1‑150 (MQ=255)

                                                                                                 tCTGTGTCGATAAACCGCTGGCAGAAAATCTGCGCGATGCTGAACGGCTGGTGGAACTGGCGGCGCGGAAAAAACTGACGTTGATGGTCGGTTTTAACCGTCGTTTCGCACCACTCTACGGTGAGTTAAAAACGCAACTCGCCACCGCAg                                                     <  1:3171755/150‑1 (MQ=255)

                                                                                                   tgtgTCGATAAACCGCTGGCAGAAAATCTGCGCGATGCTGAACGGCTGGTGGAACTGGCGGCGCGGAAAAAACTGACGTTGATGGTCGGTTTTAACCGTCGTTTCGCACCACTCTACGGTGAGTTAAAAACGCAACTCGCCACCGCAGcc                                                   >  2:3203187/1‑150 (MQ=255)

                                                                                                     tgtCGATAAACCGCTGGCAGAAAATCTGCGCGATGCTGAACGGCTGGTGGAACTGGCGGCGCGGAAAAAACTGACGTTGATGGTCGGTTTTAACCGTCGTTTCGCACCACTCTACGGTGAGTTAAAAACGCAACTCGCCACCGCAGCCTc                                                 >  2:3588364/1‑150 (MQ=255)

                                                                                                     tgtCGATAAACCGCTGGCAGAAAATCTGCGCGATGCTGAACGGCTGGTGGAACTGGCGGCGCGGAAAAAACTGACGTTGATGGTCGGTTTTAACCGTCGTTTCGCACCACTCTACGGTGAGTTAAAAACGCAACTCGCCACCGCAGCCTc                                                 >  1:670953/1‑150 (MQ=255)

                                                                                                      gtCGATAAACCGCTGGCAGAAAATCTGCGCGATGCTGAACGGCTGGTGGAACTGGCGGCGCGGAAAAAACTGACGTTGATGGTCGGTTTTAACCGTCGTTTCGCACCACTCTACGGTGAGTTAAAAACGCAACTCGCCACCGCAGCCTCg                                                <  2:2440803/150‑1 (MQ=255)

                                                                                                       tCGATAAACCGCTGGCAGAAAATCTGCGCGATGCTGAACGGCTGGTGGAACTGGCGGCGCGGAAAAAACTGACGTTGATGGTCGGTTTTAACCGTCGTTTCGCACCACTCTACGGTGAGTTAAAAACGCAACTCGCCACCGCAGCCTCGc                                               >  2:1729387/1‑150 (MQ=255)

                                                                                                       tCGATAAACCGCTGGCAGAAAATCTGCGCGATGCTGAACGGCTGGTGGAACTGGCGGCGCGGAAAAAACTGACGTTGATGGTCGGTTTTAACCGTCGTTTCGCACCACTCTACGGTGAGTTAAAAACGCAACTCGCCACCGCAGCCTCGc                                               >  1:1496055/1‑150 (MQ=255)

                                                                                                        cGATAAACCGCTGGCAGAAAATCTGCGCGATGCTGAACGGCTGGTGGAACTGGCGGCGCGGAAAAAACTGACGTTGATGGTCGGTTTTAACCGTCGTTTCGCACCACTCTACGGTGAGTTAAAAACGCAACTCGCCACCGCAGCCTCGCt                                              >  1:4529309/1‑150 (MQ=255)

                                                                                                         gATAAACCGCTGGCAGAAAATCTGCGCGATGCTGAACGGCTGGTGGAACTGGCGGCGCGGAAAAAACTGACGTTGATGGTCGGTTTTAACCGTCGTTTCGCACCACTCTACGGTGAGTTAAAAACGCAACTCGCCACCg                                                        <  1:2127260/139‑1 (MQ=255)

                                                                                                         gATAAACCGCTGGCAGAAAATCTGCGCGATGCTGAACGGCTGGTGGAACTGGCGGCGCGGAAAAAACTGACGTTGATGGTCGGTTTTAACCGTCGTTTCGCACCACTCTACGGTGAGTTAAAAACGCAACTCGCCACCGCAGCCTCGCTa                                             <  2:4842117/150‑1 (MQ=255)

                                                                                                         gATAAACCGCTGGCAGAAAATCTGCGCGATGCTGAACGGCTGGTGGAACTGGCGGCGCGGAAAAAACTGACGTTGATGGTCGGTTTTAACCGTCGTTTCGCACCACTCTACGGTGAGTTAAAAACGCAACTCGCCACCGCAGCCTCGCTa                                             >  2:5082457/1‑150 (MQ=255)

                                                                                                         gATAAACCGCTGGCAGAAAATCTGCGCGATGCTGAACGGCTGGTGGAACTGGCGGCGCGGAAAAAACTGACGTTGATGGTCGGTTTTAACCGTCGTTTCGCACCACTCTACGGTGAGTTAAAAACGCAACTCGCCACCGCAGCCTCGCTa                                             >  1:4817855/1‑150 (MQ=255)

                                                                                                          aTAAACCGCTGGCAGAAAATCTGCGCGATGCTGAACGGCTGGTGGAACTGGCGGCGCGGAAAAAACTGACGTTGATGGTCGGTTTTAACCGTCGTTTCGCACCACTCTACGGTGAGTTAAAAACGCAACTCGCCACCGCAGCCTCGCTaa                                            >  1:4660908/1‑150 (MQ=255)

                                                                                                           tAAACCGCTGGCAGAAAATCTGCGCGATGCTGAACGGCTGGTGGAACTGGCGGCGCGGAAAAAACTGACGTTGATGGTCGGTTTTAACCGTCGTTTCGCACCACTCTACGGTGAGTTAAAAACGCAACTCGCCACCGCAGCCTCGCTAAg                                           >  1:1735968/1‑150 (MQ=255)

                                                                                                            aaaCCGCTGGCAGAAAATCTGCGCGATGCTGAACGGCTGGTGGAACTGGCGGCGCGGAAAAAACTGACGTTGATGGTCGGTTTTAACCGTCGTTTCGCACCACTCTACGGTGAGTTAAAAACGCAACTCGCCACCGCAGCCTCGCTAAGa                                          <  2:1070479/150‑1 (MQ=255)

                                                                                                               ccGCTGGCAGAAAATCTGCGCGATGCTGAACGGCTGGTGGAACTGGCGGCGCGGAAAAAACTGACGTTGATGGTCGGTTTTAACCGTCGTTTCGCACCACTCTACGGTGAGTTAAAAACGCAACTCGCCACCGCAGCCTCGCTAAGAATg                                       <  1:1934254/150‑1 (MQ=255)

                                                                                                                 gCTGGCAGAAAATCTGCGCGATGCTGAACGGCTGGTGGAACTGGCGGCGCGGAAAAAACTGACGTTGATGGTCGGTTTTAACCGTCGTTTCGCACCACTCTACGGTGAGTTAAAAACGCAACTCGCCACCGCAGCCTCGCTAAGAATGGa                                     <  1:260296/150‑1 (MQ=255)

                                                                                                                 gCTGGCAGAAAATCTGCGCGATGCTGAACGGCTGGTGGAACTGGCGGCGCGGAAAAAACTGACGTTGATGGTCGGTTTTAACCGTCGTTTCGCACCACTCTACGGTGAGTTAAAAACGCAACTCGCCACCGCAGCCTCGCTAAGAATGGa                                     >  1:844782/1‑150 (MQ=255)

                                                                                                                    ggCAGAAAATCTGCGCGATGCTGAACGGCTGGTGGAACTGGCGGCGCGGAAAAAACTGACGTTGATGGTCGGTTTTAACCGTCGTTTCGCACCACTCTACGGTGAGTTAAAAACGCAACTCGCCACCGCAGCCTCGCTAAGAATGGATaa                                  <  2:2075088/150‑1 (MQ=255)

                                                                                                                      cAGAAAATCTGCGCGATGCTGAACGGCTGGTGGAACTGGCGGCGCGGAAAAAACTGACGTTGATGGTCGGTTTTAACCGTCGTTTCGCACCACTCTACGGTGAGTTAAAAACGCAACTCGCCACCGCAGCCTCGCTAAGAATGGATAAAc                                <  1:3590855/150‑1 (MQ=255)

                                                                                                                         aaaaTCTGCGCGATGCTGAACGGCTGGTGGAACTGGCGGCGCGGAAAAAACTGACGTTGATGGTCGGTTTTAACCGTCGTTTCGCACCACTCTACGGTGAGTTAAAAACGCAACTCGCCACCGCAGCCTCGCTAAGAATGGATAAACATc                             >  2:778968/1‑150 (MQ=255)

                                                                                                                            aTCTGCGCGATGCTGAACGGCTGGTGGAACTGGCGGCGCGGAAAAAACTGACGTTGATGGTCGGTTTTAACCGTCGTTTCGCACCACTCTACGGTGAGTTAAAAACGCAACTCGCCACCGCAGCCTCGCTAAGAATGGATAAACATCGTa                          >  2:835556/1‑150 (MQ=255)

                                                                                                                               tGCGCGATGCTGAACGGCTGGTGGAACTGGCGGCGCGGAAAAAACTGACGTTGATGGTCGGTTTTAACCGTCGTTTCGCACCACTCTACGGTGAGTTAAAAACGCAACTCGCCACCGCAGCCTCGCTAAGAATGGATAAACATCGTAGCa                       <  2:3089561/150‑1 (MQ=255)

                                                                                                                                gcgcGATGCTGAACGGCTGGTGGAACTGGCGGCGCGGAAAAAACTGACGTTGATGGTCGGTTTTAACCGTCGTTTCGCACCACTCTACGGTGAGTTAAAAACGCAACTCGCCACCGCAGCCTCGCTAAGAATGGATAAACATCGTAGCaa                      <  1:1766200/150‑1 (MQ=255)

                                                                                                                                 cgcgATGCTGAACGGCTGGTGGAACTGGCGGCGCGGAAAAAACTGACGTTGATGGTCGGTTTTAACCGTCGTTTCGCACCACTCTACGGTGAGTTAAAAACGCAACTCGCCACCGCAGCCTCGCTAAGAATGGATAAACATCGTAGCAAt                     <  2:3915667/150‑1 (MQ=255)

                                                                                                                                 cgcgATGCTGAACGGCTGGTGGAACTGGCGGCGCGGAAAAAACTGACGTTGATGGTCGGTTTTAACCGTCGTTTCGCACCACTCTACGGTGAGTTAAAAACGCAACTCGCCACCGCAGCCTCGCTAAGAATGGATAAACATCGTAGCAAt                     <  2:1311068/150‑1 (MQ=255)

                                                                                                                                  gcgATGCTGAACGGCTGGTGGAACTGGCGGCGCGGAAAAAACTGACGTTGATGGTCGGTTTTAACCGTCGTTTCGCACCACTCTACGGTGAGTTAAAAACGCAACTCGCCACCGCAGCCTCGCTAAGAATGGATAAACATCGTAGCAATa                    <  1:1610623/150‑1 (MQ=255)

                                                                                                                                    gATGCTGAACGGCTGGTGGAACTGGCGGCGCGGAAAAAACTGACGTTGATGGTCGGTTTTAACCGTCGTTTCGCACCACTCTACGGTGAGTTAAAAACGCAACTCGCCACCGCAGCCTCGCTAAGAATGGATAAACATCGTAGCAATAGc                  >  1:2371074/1‑150 (MQ=255)

                                                                                                                                    gATGCTGAACGGCTGGTGGAACTGGCGGCGCGGAAAAAACTGACGTTGATGGTCGGTTTTAACCGTCGTTTCGCACCACTCTACGGTGAGTTAAAAACGCAACTCGCCACCGCAGCCTCGCTAAGAATGGATAAACATCGTAGCAATAGc                  <  1:3870288/150‑1 (MQ=255)

                                                                                                                                       gCTGAACGGCTGGTGGAACTGGCGGCGCGGAAAAAACTGACGTTGATGGTCGGTTTTAACCGTCGTTTCGCACCACTCTACGGTGAGTTAAAAACGCAACTCGCCACCGCAGCCTCGCTAAGAATGGATAAACATCGTAGCAATAGCGTc               <  1:2977196/150‑1 (MQ=255)

                                                                                                                                       gCTGAACGGCTGGTGGAACTGGCGGCGCGGAAAAAACTGACGTTGATGGTCGGTTTTAACCGTCGTTTCGCACCACTCTACGGTGAGTTAAAAACGCAACTCGCCACCGCAGCCTCGCTAAGAATGGATAAACATCGTAGCAATAGCGTc               <  2:4762724/150‑1 (MQ=255)

                                                                                                                                         tGAACGGCTGGTTGAACTGGCGGCGCGGAAAAAACTGACGTTGATGGTCGGTTTTAACCGTCGTTTCGCACCACTCTACGGTGAGTTAAAAACGCAACTCGCCACCGCAGCCTCGCTAAGAATGGATAAACATCGTAGCAATAGCGTCgg             <  2:101718/150‑1 (MQ=255)

                                                                                                                                         tGAACGGCTGGTGGAACTGGCGGCGCGGAAAAAACTGACGTTGATGGTCGGTTTTAACCGTCGTTTCGCACCACTCTACGGTGAGTTAAAAACGCAACTCGCCACCGCAGCCTCGCTAAGAATGGATAAACATCGTAGCAATAGCGTCgg             >  1:4999948/1‑150 (MQ=255)

                                                                                                                                         tGAACGGCTGGTGGAACTGGCGGCGCGGAAAAAACTGACGTTGATGGTCGGTTTTAACCGTCGTTTCGCACCACTCTACGGTGAGTTAAAAACGCAACTCGCCACCGCAGCCTCGCTAAGAATGGATAAACATCGTAGCAATAGCGTCgg             >  1:281925/1‑150 (MQ=255)

                                                                                                                                         tGAACGGCTGGTGGAACTGGCGGCGCGGAAAAAACTGACGTTGATGGTCGGTTTTAACCGTCGTTTCGCACCACTCTACGGTGAGTTAAAAACGCAACTCGCCACCGCAGCCTCGCTAAGAATGGATAAACATCGTAGCAATAGCGTCgg             >  2:5136343/1‑150 (MQ=255)

                                                                                                                                         tGAACGGCTGGTGGAACTGGCGGCGCGGAAAAAACTGACGTTGATGGTCGGTTTTAACCGTCGTTTCGCACCACTCTACGGTGAGTTAAAAACGCAACTCGCCACCGCAGCCTCGCTAAGAATGGATAAACATCGTAGCAATAGCGTCgg             <  1:3823322/150‑1 (MQ=255)

                                                                                                                                           aaCGGCTGGTGGAACTGGCGGCGCGGAAAAAACTGACGTTGATGGTCGGTTTTAACCGTCGTTTCGCACCACTCTACGGTGAGTTAAAAACGCAACTCGCCACCGCAGCCTCGCTAAGACTGGATAAACATCGTAGCAATAGCGTCGGGc           >  1:4113695/1‑150 (MQ=255)

                                                                                                                                              ggCTGGTGGAACTGGCGGCGCGGAAAAAACTGACGTTGATGGTCGGTTTTAACCGTCGTTTCGCACCACTCTACGGTGAGTTAAAAACGCAACTCGCCACCGCAGCCTCGCTAAGAATGGATAAACATCGTAGCAATAGCGTCGGGCcac        <  2:2538659/150‑1 (MQ=255)

                                                                                                                                                  ggtggAACTGGCGGCGCGGAAAAAACTGACGTTGATGGTCGGTTTTAACCGTCGTTTCGCACCACTCTACGGTGAGTTAAAAACGCAACTCGCCACCGCAGCCTCGCTAAGAATGGATAAACATCGTAGCAATAGCGTCGGGCCACACGa    >  2:1291477/1‑150 (MQ=255)

                                                                                                                                                   gtggAACTGGCGGCGCGGAAAAAACTGACGTTGATGGTCGGTTTTAACCGTCGTTTCGCACCACTCTACGGTGAGTTAAAAACGCAACTCGCCACCGCAGCCTCGCTAAGAATGGATAAACATCGTAGCAATAGCGTCGGGCCACACGAt   <  2:2368391/150‑1 (MQ=255)

TCCAGCCTTGCCGCCAGTTGCGATGCGGTTTTTGTGCATTCCAGCACCGCCAGCCACTTTGACGTGGTCAGTACGTTACTCAATGCGGGGGTACATGTCTGTGTCGATAAACCGCTGGCAGAAAATCTGCGCGATGCTGAACGGCTGGTGGAACTGGCGGCGCGGAAAAAACTGACGTTGATGGTCGGTTTTAACCGTCGTTTCGCACCACTCTACGGTGAGTTAAAAACGCAACTCGCCACCGCAGCCTCGCTAAGAATGGATAAACATCGTAGCAATAGCGTCGGGCCACACGATC  >  CP009273/1122433‑1122730

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`