Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,152,278 G→T 92.2% E16* (GAA→TAA)  ycfH → putative DNase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,152,2780GT92.2% 991.1 / 70.3 332E16* (GAA→TAA) ycfHputative DNase
Reads supporting (aligned to +/- strand):  ref base G (10/16);  new base T (172/133);  total (182/150)
Fisher's exact test for biased strand distribution p-value = 1.00e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.63e-01

Only 100 of 339 total aligned reads displayed.

CATCAACCGCGAGCTTCTCATCACCGATCTTTTACTGCGTATTGAGCATTACCTGCAACCGGGCGTTGTGCTACCGGTTCCTCATCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATGAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCGGGTTATTTACATATGCGGGATCTGGTAGGCGAACGTGACAACGTCGTATTTTCTTGTGG  >  CP009273/1152132‑1152426
                                                                                                                                                  |                                                                                                                                                    
cATCAACCGCGAGCTTCTCATCACCGATCTTTTACTGCGTATTGAGCATTACCTGCAACCGGGCGTTGTGCTACCGGTTCCTCATCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATTAAt                                                                                                                                                   >  2:2237102/1‑150 (MQ=255)
 aTCAACCGCGAGCTTCTCATCACCGATCTTTTACTGCGTATTGAGCATTACCTGCAACCGGGCGTTGTGCTACCGGTTCCTCATCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATTAATc                                                                                                                                                  >  2:1173131/1‑150 (MQ=255)
        gcgAGCTTCTCATCACCGATCTTTTACTGCGTATTGAGCATTACCTGCAACCGGGCGTTGTGCTACCGGTTCCTCATCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATTAATCTTTGCAt                                                                                                                                           <  1:527513/150‑1 (MQ=255)
        gcgAGCTTCTCATCACCGATCTTTTACTGCGTATTGAGCATTACCTGCAACCGGGCGTTGTGCTACCGGTTCCTCATCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATTAATCTTTGCAt                                                                                                                                           <  2:1096037/150‑1 (MQ=255)
         cgAGCTTCTCATCACCGATCTTTTGCTGCGTATTGAGCATTACCTGCAACCGGGCGTTGTGCTACCGGTTCCTCATCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATTAATCTTTGCATa                                                                                                                                          <  1:1514414/150‑1 (MQ=255)
         cgAGCTTCTCATCACCGATCTTTTACTGCGTATTGAGCATTACCTGCAACCGGGCGTTGTGCTACCGGTTCCTCATCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATTAATCTTTGCATa                                                                                                                                          >  2:4204072/1‑150 (MQ=255)
          gAGCTTCTCATCACCGATCTTTTACTGCGTATTGAGCATTACCTGCAACCGGGCGTTGTGCTACCGGTTCCTCATCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATTAATCTTTGCATaa                                                                                                                                         >  2:3551385/1‑150 (MQ=255)
           aGCTTCTCATCACCGATCTTTTACTGCGTATTGAGCATTACCTGCAACCGGGCGTTGTGCTACCGGTTCCTCATCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATTAATCTTTGCATAAg                                                                                                                                        >  1:4975605/1‑150 (MQ=255)
           aGCTTCTCATCACCGATCTTTTACTGCGTATTGAGCATTACCTGCAACCGGGCGTTGTGCTACCGGTTCCTCATCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATTAATCTTTGCATAAg                                                                                                                                        >  1:2526124/1‑150 (MQ=255)
             cTTCTCATCACCGATCTTTTACTGCGTATTGAGCATTACCTGCAACCGGGCGTTGTGCTACCGGTTCCTCATCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATTAATCTTTGCATAAGGa                                                                                                                                      >  1:3779073/1‑150 (MQ=255)
               tctcATCACCGATCTTTTACTGCGTATTGAGCATTACCTGCAACCGGGCGTTGTGCTGCCGGTTCCTCATCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATTAATCTTTGCATAAGGACg                                                                                                                                    >  2:5102954/1‑150 (MQ=255)
                 tcatcaCCGATCTTTTACTGCGTATTGAGCATTACCTGCAACCGGGCGTTGTGCTACCGGTTCCTCATCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATTAATCTTTGCATAAGGACGTg                                                                                                                                  <  1:2931943/150‑1 (MQ=255)
                 tcatcaCCGATCTTTTACTGCGTATTGAGCATTACCTGCAACCGGGCGTTGTGCTACCGGTTCCTCATCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATTAATCTTTGCATAAGGACGTg                                                                                                                                  >  2:4878056/1‑150 (MQ=255)
                 tcatcaCCGATCTTTTACTGCGTATTGAGCATTACCTGCAACCGGGCGTTGTGCTACCGGTTCCTCATCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATTAATCTTTGCATAAGGACGTg                                                                                                                                  >  1:4870206/1‑150 (MQ=255)
                  catcaCCGATCTTTTACTGCGTATTGAGCATTACCTGCAACCGGGCGTTGTGCTACCGGTTCCTCATCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATTAATCTTTGCATAAGGACGTgg                                                                                                                                 >  2:217544/1‑150 (MQ=255)
                  catcaCCGATCTTTTACTGCGTATTGAGCATTACCTGCAACCGGGCGTTGTGCTACCGGTTCCTCATCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATTAAAATTTGCATAAGGACGTgg                                                                                                                                 >  1:1754718/1‑150 (MQ=255)
                     caCCGATCTTTTACTGCGTATTGAGCATTACCTGCAACCGGGCGTTGTGCTACCGGTTCCTCATCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATTAATCTTTGCATAAGGACGTGGATg                                                                                                                              <  2:1832303/150‑1 (MQ=255)
                      aCCGATCTTTTACTGCGTATTGAGCATTACCTGCAACCGGGCGTTGTGCTACCGGTTCCTCATCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATTAATCTTTGCATAAGGACGTGGATGa                                                                                                                             >  1:1811845/1‑150 (MQ=255)
                         gATCTTTTACTGCGTATTGAGCATTACCTGCAACCGGGCGTTGTGCTACCGGTTCCTCATCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATTAATCTTTGCATAAGGACGTGGATGACGt                                                                                                                          >  1:2045242/1‑150 (MQ=255)
                         gATCTTTTACTGCGTATTGAGCATTACCTGCAACCGGGCGTTGTGCTACCGGTTCCTCATCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATTAATCTTTGCATAAGGACGTGGATGACGt                                                                                                                          <  2:2538544/150‑1 (MQ=255)
                            cTTTTACTGCGTATTGAGCATTACCTGCAACCGGGCGTTGTGCTACCGGTTCCTCATCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATTAATCTTTGCATAAGGACGTGGATGACGTTCt                                                                                                                       <  2:2732420/150‑1 (MQ=255)
                             ttttACTGCGTATTGAGCATTACCTGCAACCGGGCGTTGTGCTACCGGTTCCTCATCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATTAATCTTTGCATAAGGACGTGGATGACGTTCTg                                                                                                                      >  2:4264260/1‑150 (MQ=255)
                              tttACTGCGTATTGAGCATTACCTGCAACCGGGCGTTGTGCTACCGGTTCCTCATCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATCAATCTTTGCATAAGGACGTGGATGACGTTCTgg                                                                                                                     <  1:1224429/150‑1 (MQ=255)
                               ttACTGCGTATTGAGCATTACCTGCAACCGGGCGTTGTGCTACCGGTTCCTCATCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATGAATCTTTGCATAAGGACGTGGATGACGTTCTGGc                                                                                                                    <  1:2376167/150‑1 (MQ=255)
                                   tGCGTATTGAGCATTACCTGCAACCGGGCGTTGTGCTACCGGTTCCTCATCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATTAATCTTTGCATAAGGACGGGGATGACGTTCTGGCGaaa                                                                                                                >  2:3140258/1‑150 (MQ=255)
                                     cGTATTGAGCATTACCTGCAACCGGGCGTTGTGCTACCGGTTCCTCATCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATTAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAgc                                                                                                              <  2:2664717/150‑1 (MQ=255)
                                     cGTATTGAGCATTACCTGCAACCGGGCGTTGTGCTACCGGTTCCTCATCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATTAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAgc                                                                                                              >  1:104771/1‑150 (MQ=255)
                                      gTATTGAGCATTACCTGCAACCGGGCGTTGTGCTACCGGTTCCTCATCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATTAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAgcc                                                                                                             >  2:1293137/1‑150 (MQ=255)
                                         ttGAGCATTACCTGCAACCGGGCGTTGTGCTACCGGTTCCTCATCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATTAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAgccgcc                                                                                                          >  2:2054017/1‑150 (MQ=255)
                                         ttGAGCATTACCTGCAACCGGGCGTTGTGCTACCGGTTCCTCATCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATTAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAgccgcc                                                                                                          <  1:284514/150‑1 (MQ=255)
                                         ttGAGCATTACCTGCAACCGGGCGTTGTGCTACCGGTTCCTCATCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATTAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAgccgcc                                                                                                          <  1:1968871/150‑1 (MQ=255)
                                          tGAGCATTACCTGCAACCGGGCGTTGTGCTACCGGTTCCTCATCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATTAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGccgccg                                                                                                         >  1:4281761/1‑150 (MQ=255)
                                           gAGCATTACCTGCAACCGGGCGTTGTGCTACCGGTTCCTCATCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATTAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCcgccgc                                                                                                        >  1:4106902/1‑150 (MQ=255)
                                            aGCATTACCTGCAACCGGGCGTTGTGCTACCGGTTCCTCATCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATTAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCa                                                                                                       <  2:269496/150‑1 (MQ=255)
                                            aGCATTACCTGCAACCGGGCGTTGTGCTACCGGTTCCTCATCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATTAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCa                                                                                                       >  2:4297164/1‑150 (MQ=255)
                                              cATTACCTGCAACCGGGCGTTGTGCTACCGGTTCCTCATCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATTAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCAcg                                                                                                     >  1:4515607/1‑150 (MQ=255)
                                                ttACCTGCAACCGGGCGTTGTGCTACCGGTTCCTCATCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGACTGGATTATTAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCAGCCGCAcgcg                                                                                                   >  2:565960/1‑150 (MQ=255)
                                                  aCCTGCTACCGGGCGTTGTGCTACCGGTTCCTCATCTTTAAGAGAGACATCATGTTTTTAGTCGACTCTCACTGCCATCTCGTTGGTCTGGATTATTAATCTTTGCATAAGGTCGTGGATGACGTTCTGGCGAAATCCGCCGCACGCGAt                                                                                                 <  2:1348806/150‑1 (MQ=255)
                                                    cTGCAACCGGGCGTTGTGCTACCGGTTCCTCATCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATTAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGAtgt                                                                                               <  1:2720992/150‑1 (MQ=255)
                                                     tGCAACCGGGCGTTGTGCTACCGGTTCCTCATCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATTAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGAtgtg                                                                                              >  2:3054748/1‑150 (MQ=255)
                                                        aaCCGGGCGTTGTGCTACCGGTTCCTCATCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATTAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGaaa                                                                                           >  1:3145576/1‑150 (MQ=255)
                                                        aaCCGGGCGTTGTGCTACCGGTTCCTCATCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATTAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGaaa                                                                                           >  2:3930720/1‑150 (MQ=255)
                                                         aCCGGGCGGTGGGCGACCGGGTCCTCCTCTTTAAGAGAGACAACCTGTTTTTAGTCGACTCACACTGCCAACTCGATGGTCTGGATTATTAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAAt                                                                                          <  1:4756411/150‑1 (MQ=255)
                                                          ccGGGCGTTGTGCTACCGGTTCCTCATCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATTAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAAtt                                                                                         <  2:1597943/150‑1 (MQ=255)
                                                            gggCGTTGTGCTACCGGTTCCTCATCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATTAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAAtttt                                                                                       >  1:363865/1‑150 (MQ=255)
                                                               ctttGTGCTACCGGTTCCTCATCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATGAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTc                                                                                    >  1:3488849/3‑150 (MQ=255)
                                                                gTTGTGCTACCGGTTCCTCATCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATTAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCt                                                                                   <  2:267879/150‑1 (MQ=255)
                                                                gTTGTGCTACCGGTTCCTCATCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATTAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCt                                                                                   <  1:3597710/150‑1 (MQ=255)
                                                                gTTGTGCTACCGGTTCCTCATCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATGAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCt                                                                                   <  2:4694174/150‑1 (MQ=255)
                                                                  tgtgCTACCGGTTCCTCATCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATTAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTgg                                                                                 <  2:1505227/150‑1 (MQ=255)
                                                                    tgCTACCGGTTCCTCATCTTTAAGATAGACATCATGTTTTTAGTCGACTAACACTGCCATCTCGATGGTCTGGATTATTAATCTTTGCATAAGGACGTGGATGAAGTTCTGGCGAAAGCCGCCGAACGCGATGTGAAATTTTGTATggaa                                                                               >  2:1030078/1‑148 (MQ=255)
                                                                     gCTACCGGTTCCTCATCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATTAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAg                                                                              <  1:1927085/150‑1 (MQ=255)
                                                                       tACCGGGTCCTCATCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATTAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTc                                                                            <  1:4705749/150‑1 (MQ=255)
                                                                        aCCGGTTCCTCATCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATTAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCg                                                                           <  2:4428718/150‑1 (MQ=255)
                                                                           ggTTCCTCATCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATTAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCca                                                                        >  2:3066171/1‑150 (MQ=255)
                                                                            gTTCCTCATCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATTAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCcac                                                                       <  1:4373727/150‑1 (MQ=255)
                                                                             ttCCTCATCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATTAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCcaca                                                                      >  2:5119206/1‑150 (MQ=255)
                                                                                cTCATCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATTAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCacaaca                                                                   >  2:2492397/1‑150 (MQ=255)
                                                                                cTCATCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATTAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCacaaca                                                                   >  2:722918/1‑150 (MQ=255)
                                                                                cTCATCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATTAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCacaaca                                                                   >  1:778585/1‑150 (MQ=255)
                                                                                 tCATCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATTAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACAt                                                                  >  2:3863727/1‑150 (MQ=255)
                                                                                      tttAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATTAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCg                                                             <  1:2438493/150‑1 (MQ=255)
                                                                                       ttAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATTAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCgg                                                            <  2:268049/150‑1 (MQ=255)
                                                                                         aaGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATTAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCGGGt                                                          >  2:3803447/1‑150 (MQ=255)
                                                                                          agagagACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATTAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCGGGtt                                                         >  1:1408938/1‑150 (MQ=255)
                                                                                           gagagaCATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATTAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCGGGTTa                                                        <  2:4875733/150‑1 (MQ=255)
                                                                                            agagaCATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATTAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCGGGTTAt                                                       >  2:1975989/1‑150 (MQ=255)
                                                                                            agagaCATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATTAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCGGGTTAt                                                       >  2:4529588/1‑150 (MQ=255)
                                                                                                     atGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATTAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCGGGTTATTTACATATg                                              >  1:1818004/1‑150 (MQ=255)
                                                                                                      tGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATTAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCATAACATTACCGGGTTATTTACATATGc                                             <  1:1317297/150‑1 (MQ=255)
                                                                                                            tAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATTAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCGGGTTATTTACATATGCGGGATc                                       >  2:3702930/1‑150 (MQ=255)
                                                                                                            tAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATTAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCGGGTTATTTACATATGCGGGATc                                       >  2:3989828/1‑150 (MQ=255)
                                                                                                            tAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATTAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCGGGTTATTTACATATGCGGGATc                                       >  1:3027736/1‑150 (MQ=255)
                                                                                                            tAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATTAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCGGGTTATTTACATATGCGGGATc                                       >  2:3321746/1‑150 (MQ=255)
                                                                                                             aGTCGACTCACTCTGCCATCTCGTTGGTCTGGAGTATTAATCTTTGCATACGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCTATGTGAATTTTTGTCTGGCAGTCGCCACAACATTTCCGGGTTATTTACATATGCGGGATCt                                      <  1:2913959/150‑1 (MQ=255)
                                                                                                              gTCGACTCACACTGCCATCTCGATGGTCTGGATTATTAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCGGGTTATTTACATATGCGGGATCTg                                     <  1:625903/150‑1 (MQ=255)
                                                                                                               tCGACTCACACTGCCATCTCGATGGTCTGGATTATTAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCGGGTTATTTACATATGCGGGATCTgg                                    >  2:2858816/1‑150 (MQ=255)
                                                                                                                 gACTCACACTGCCATCTCGATGGTCTGGATTATTAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCGGGTTATTTACATATGCGGGATCTGGTa                                  >  1:3213928/1‑150 (MQ=255)
                                                                                                                 gACTCACACTGCCATCTCGATGGTCTGGATTATTAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCAAGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCGGGTTATTTACATATGCGGGATCTGGTa                                  <  1:3377073/150‑1 (MQ=255)
                                                                                                                      acacTGCCATCTCGATGGTCTGGATTATTAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCGGGTTATTTACATATGCGGGATCTGGTAGGCGa                             >  2:3222183/1‑150 (MQ=255)
                                                                                                                        acTGCCATCTCGATGGTCTGGATTATTAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCGGGTTATTTACATATGCGGGATCTGGTAGGCGAAc                           <  2:2016975/150‑1 (MQ=255)
                                                                                                                        acTGCCATCTCGATGGTCTGGATTATTAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCGGGTTATTTACATATGCGGGATCTGGTAGGCGAAc                           <  2:1258621/150‑1 (MQ=255)
                                                                                                                           gCCATCTCGATGGTCTGGATTATGAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCGGGTTATTTACATATGGGGGATCTGGTAGGCGAACGTg                        <  1:943271/150‑1 (MQ=255)
                                                                                                                             cATCTCGATGGTCTGGATTATTAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCGGGTTATTTACATATGCGGGATCTGGTAGGCGAACGTGAc                      <  1:2465623/150‑1 (MQ=255)
                                                                                                                              ctctcGATGGTCTGGATTATTAATCTGTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCGGGTTATTTACATATGCGGGATCTGGTAGGCGAACGTGACa                     <  2:1410871/149‑1 (MQ=255)
                                                                                                                              aTCTCGATGGTCTGGATTATTAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCGGGTTATTTACATATGCGGGATCTGGTAGGCGAACGTGACa                     <  1:1590058/150‑1 (MQ=255)
                                                                                                                                ctcGATGGTCTGGATTATTAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCGGGTTATTTACATATGCGGGATCTGGTAGGCGAACGTGACAAc                   <  2:1645390/150‑1 (MQ=255)
                                                                                                                                ctcGATGGTCTGGATTATTAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCGGGTTATTTACATATGCGGGATCTGGTAGGCGAACGTGACAAc                   >  1:4221600/1‑150 (MQ=255)
                                                                                                                                 tcGATGGTCTGGATTATTAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCGGGTTATTTACATATGCGGGATCTGGTAGGCGAACGTGACAAcg                  <  1:3551385/150‑1 (MQ=255)
                                                                                                                                  cGATGGTCTGGATTATTAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCGGGTTATTTACATATGCGGGATCTGGTAGGCGAACGTGACAAcgt                 >  2:4672091/1‑150 (MQ=255)
                                                                                                                                   gATGGTCTGGATTATTAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCGGGTTATTTACATATGCGGGATCTGGTAGGCGAACGTGACAAcgtc                >  2:4313762/1‑150 (MQ=255)
                                                                                                                                      ggTCTGGATTATTAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCGGGTTATTTACATATGCGGGATCTGGTAGGCGAACGTGACAACGTCGTa             >  2:64092/1‑150 (MQ=255)
                                                                                                                                       gTCTGGATTATGAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCGGGTTATTTACATATGCGGGATCTGGTAGGCGAACGTGACAACGTCGTAt            <  1:3951731/150‑1 (MQ=255)
                                                                                                                                        tCTGGATTATTAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCGGGTTATTTACATATGCGGGATCTGGTAGGCGAACGTGACAACGTCGTAtt           >  1:4625437/1‑150 (MQ=255)
                                                                                                                                        tCTGGATTATTAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCGGGTTATTTACATATGCGGGATCTGGTAGGCGAACGTGACAACGTCGTAtt           <  1:860129/150‑1 (MQ=255)
                                                                                                                                         cTGGATTATTAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCGGGTTATTTACATATGCGGGATCTGGTAGGCGAACGTGACAACGTCGTAttt          <  1:4039635/150‑1 (MQ=255)
                                                                                                                                          tGGATTATTAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCGGGTTATTTACATATGCGGGATCTGGTAGGCGAACGTGACAACGTCGTAtttt         >  1:955118/1‑150 (MQ=255)
                                                                                                                                            gATTATTAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCGGGTTATTTACATATGCGGGATCTGGTAGGCGAACGTGACAACGTCGTATTTTCt       <  2:4634528/150‑1 (MQ=255)
                                                                                                                                             attattAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCGGGTTATTTACATATGCGGGATCTGGTAGGCGAACGTGACAACGTCGTATTTTCtt      >  1:512638/1‑150 (MQ=255)
                                                                                                                                             attattAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCGGGTTATTTACATATGCGGGATCTGGTAGGCGAACGTGACAACGTCGTATTTTCtt      <  1:5036739/150‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                    
CATCAACCGCGAGCTTCTCATCACCGATCTTTTACTGCGTATTGAGCATTACCTGCAACCGGGCGTTGTGCTACCGGTTCCTCATCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATGAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCGGGTTATTTACATATGCGGGATCTGGTAGGCGAACGTGACAACGTCGTATTTTCTTGTGG  >  CP009273/1152132‑1152426

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑