Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,194,194 C→A 21.6% S15* (TCA→TAA)  lit → e14 prophage; cell death peptidase, inhibitor of T4 late gene expression

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,194,1940CA21.6% 408.3 / 85.8 156S15* (TCA→TAA) lite14 prophage; cell death peptidase, inhibitor of T4 late gene expression
Reads supporting (aligned to +/- strand):  ref base C (52/70);  new base A (16/18);  total (68/88)
Fisher's exact test for biased strand distribution p-value = 6.98e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.33e-01

Only 100 of 165 total aligned reads displayed.

ATGTCGATAATTCATTACGCATATAGTATCGAACATAGAAAAAACTGAAGATTCATCTTATTTTGTATATACTACCTAGCCCAACAATGTAGAGGTTAACGAAAAATGCGCTCACCAATTTGTCATCTTTTCTCAGCAATTAATTCATCACCATTTAAGATTGCACCAGAGAAGGAGCAAGATCTTAAAACGATAGTTGACGACAAAAAAATTATAATTTCAGTTGTGAGTGAACCTGGTTTTAATATCCGAGTCAGGAAGAATGAGAGTAATAATTCACATGAAATAGTTCTAACA  >  CP009273/1194046‑1194342
                                                                                                                                                    |                                                                                                                                                    
aTGTCGATAATTCATTACGCATATAGTATCGAACATAGAAAAAACTGAAGATTCATCTTATTTTGTATATACTACCTAGCCCAACAATGTAGAGGTTAACGAAAAATGCGCTCACCAATTTGTCATCTTTTCTCAGCAATTAATtcatca                                                                                                                                                     <  1:2999745/150‑1 (MQ=255)
        aaTTCATTACGCATATAGTATCGAACATAGAAAAAACTGAAGATTCATCTTATTTTGTATATACTACCTAGCCCAACAATGTAGAGGTTAACGAAAAATGCGCTCACCAATTTGTCATCTTTTCTCAGCAATTAATTCATCACCATTTaa                                                                                                                                             >  1:3809794/1‑150 (MQ=255)
        aaTTCATTACGCATATAGTATCGAACATAGAAAAAACTGAAGATTCATCTTATTTTGTATATACTACCTAGCCCAACAATGTAGAGGTTAACGAAAAATGCGCTCACCAATTTGTCATCTTTTCTCAGCAATTAATTCATCACCATTTaa                                                                                                                                             >  1:200564/1‑150 (MQ=255)
          ttCATTACGCATATAGTATCGAACATAGAAAAAACTGAAGATTCATCTTATTTTGTATATACTACCTAGCCCAACAATGTAGAGGTTAACGAAAAATGCGCTCACCAATTTGTCATCTTTTCTCAGCAATTAATTCATAACCATTTAAGa                                                                                                                                           <  2:2849152/150‑1 (MQ=255)
           tCATTACGCATATAGTATCGAACATAGAAAAAACTGAAGATTCATCTTATTTTGTATATACTACCTAGCCCAACAATGTAGAGGTTAACGAAAAATGCGCTCACCAATTTGTCATCTTTTCTCAGCAATTAATTCATCACCATTTAAGAt                                                                                                                                          <  2:1199686/150‑1 (MQ=255)
           tCATTACGCATATAGTATCGAACATAGAAAAAACTGAAGATTCATCTTATTTTGTATATACTACCTAGCCCAACAATGTAGAGGTTAACGAAAAATGCGCTCACCAATTTGTCATCTTTTCTCAGCAATTAATTCATCACCATTTAAGAt                                                                                                                                          <  2:1199275/150‑1 (MQ=255)
           tCATTACGCATATAGTATCGAACATAGAAAAAACTGAAGATTCATCTTATTTTGTATATACTACCTAGCCCAACAATGTAGAGGTTAACGAAAAATGCGCTCACCAATTTGTCATCTTTTCTCAGCAATTAATTCATCACCATTTAAGAt                                                                                                                                          >  2:27815/1‑150 (MQ=255)
            cATTACGCATATAGTATCGAACATAGAAAAAACTGAAGATTCATCTTATTTTGTATATACTACCTAGCCCAACAATGTAGAGGTTAACGAAAAATGCGCTCACCAATTTGTCATCTTTTCTCAGCAATTAATTCATAACCATTTAAGAtt                                                                                                                                         >  1:2694482/1‑150 (MQ=255)
               tACGCATATAGTATCGAACATAGAAAAAACTGAAGATTCATCTTATTTTGTATATACTACCTAGCCCAACAATGTAGAGGTTAACGAAAAATGCGCTCACCAATTTGTCATCTTTTCTCAGCAATTAATTCATAACCATTTAAGATTGCa                                                                                                                                      >  2:3298514/1‑150 (MQ=255)
                   cATATAGTATCGAACATAGAAAAAACTGAAGATTCATCTTATTTTGTATATACTACCTAGCCCAACAATGTAGAGGTTAACGAAAAATGCGCTCACCAATTTGTCATCTTTTCTCAGCAATTAATTCATAACCATTTAAGATTGCACCag                                                                                                                                  <  1:3876831/150‑1 (MQ=255)
                     tataGTATCGAACATAGAAAAAACTGAAGATTCATCTTATTTTGTATATACTACCTAGCCCAACAATGTAGAGGTTAACGAAAAATGCGCTCACCAATTTGTCATCTTTTCTCAGCAATTAATTCATCACCATTTAAGATTGCACCagag                                                                                                                                <  1:3031634/150‑1 (MQ=255)
                     tataGTATCGAACATAGAAAAAACTGAAGATTCATCTTATTTTGTATATACTACCTAGCCCAACAATGTAGAGGTTAACGAAAAATGCGCTCACCAATTTGTCATCTTTTCTCAGCAATTAATTCATCACCATTTAAGATTGCACCagag                                                                                                                                <  1:2198415/150‑1 (MQ=255)
                     tataGTATCGAACATAGAAAAAACTGAAGATTCATCTTATTTTGTATATACTACCTAGCCCAACAATGTAGAGGTTAACGAAAAATGCGCTCACCAATTTGTCATCTTTTCTCAGCAATTAATTCATCACCATTTAAGATTGCACCagag                                                                                                                                <  2:4762418/150‑1 (MQ=255)
                      ataGTATCGAACATAGAAAAAACTGAAGATTCATCTTATTTTGTATATACTACCTAGCCCAACAATGTAGAGGTTAACGAAAAATGCGCTCACCAATTTGTCATCTTTTCTCAGCAATTAATTCATAACCATTTAAGATTGCACCAgaga                                                                                                                               >  1:3125242/1‑150 (MQ=255)
                       taGTATCGAACATAGAAAAAACTGAAGATTCATCTTATTTTGTATATACTACCTAGCCCAACAATGTAGAGGTTAACGAAAAATGCGCTCACCAATTTGTCATCTTTTCTCAGCAATTAATTCATAACCATTTAAGATTGCACCAGAGaa                                                                                                                              >  2:4305122/1‑150 (MQ=255)
                         gTATCGAACATAGAAAAAACTGAAGATTCATCTTATTTTGTATATACTACCTAGCCCAACAATGTAGAGGTTAACGAAAAATGCGCTCACCAATTTGTCATCTTTTCTCAGCAATTAATTCATAACCATTTAAGATTGCACCAGAGAAgg                                                                                                                            <  2:2180473/150‑1 (MQ=255)
                          tATCGAACATAGAAAAAACTGAAGATTCATCTTATTTTGTATATACTACCTAGCCCAACAATGTAGAGGTTAACGAAAAATGCGCTCACCAATTTGTCATCTTTTCTCAGCAATTAATTCATCACCATTTAAGATTGCACCAGAGAAGGa                                                                                                                           <  2:8566/150‑1 (MQ=255)
                          tATCGAACATAGAAAAAACTGAAGATTCATCTTATTTTGTATATACTACCTAGCCCAACAATGTAGAGGTTAACGAAAAATGCGCTCACCAATTTGTCATCTTTTCTCAGCAATTAATTCATCACCATTTAAGATTGCACCAGAGAAGGa                                                                                                                           <  2:749389/150‑1 (MQ=255)
                            tCGAACATAGAAAAAACTGAAGATTCATCTTATTTTGTATATACTACCTAGCCCAACAATGTAGAGGTTAACGAAAAATGCGCTCACCAATTTGTCATCTTTTCTCAGCAATTAATTCATCACCATTTAAGATTGCACCAGAGAAGGAGc                                                                                                                         <  1:2727114/150‑1 (MQ=255)
                              gAACATAGAAAAAACTGAAGATTCATCTTATTTTGTATATACTACCTAGCCCAACAATGTAGAGGTTAACGAAAAATGCGCTCACCAATTTGTCATCTTTTCTCAGCAATTAATTCATCACCATTTAAGATTGCACCAGAGAAGGAGCaa                                                                                                                       >  1:1793424/1‑150 (MQ=255)
                               aaCATAGAAAAAACTGAAGATTCATCTTATTTTGTATATACTACCTAGCCCAACAATGTAGAGGTTAACGAAAAATGCGCTCACCAATTTGTCATCTTTTCTCAGCAATTAATTCATCACCATTTAAGATTGCACCAGAGAAGGAGCAAg                                                                                                                      >  1:3245838/1‑150 (MQ=255)
                                  aTAGAAAAAACTGAAGATTCATCTTATTTTGTATATACTACCTAGCCCAACAATGTAGAGGTTAACGAAAAATGCGCTCACCAATTTGTCATCTTTTCTCAGCAATTAATTCATCACCATTTAAGATTGCACCAGAGAAGGAGCAAGATc                                                                                                                   <  1:4519098/150‑1 (MQ=255)
                                    aGAAAAAACTGAAGATTCATCTTATTTTGTATATACTACCTAGCCCAACAATGTAGAGGTTAACGAAAAATGCGCTCACCAATTTGTCATCTTTTCTCAGCAATTAATTCATAACCATTTAAGATTGCACCAGAGAAGGAGCAAGATCtt                                                                                                                 <  2:2074897/150‑1 (MQ=255)
                                    aGAAAAAACTGAAGATTCATCTTATTTTGTATATACTACCTAGCCCAACAATGTAGAGGTTAACGAAAAATGCGCTCACCAATTTGTCATCTTTTCTCAGCAATTAATTCATAACCATTTAAGATTGCACCAGAGAAGGAGCAAGATCtt                                                                                                                 <  2:209396/150‑1 (MQ=255)
                                      aaaaaaCTGAAGATTCATCTTATTTTGTATATACTACCTAGCCCAACAATGTAGAGGTTAACGAAAAATGCGCTCACCAATTTGTCATCTTTTCTCAGCAATTAATTCATCACCATTTAAGATTGCACCAGAGAAGGAGCAAGATCTTaa                                                                                                               >  1:2999390/1‑150 (MQ=255)
                                      aaaaaaCTGAAGATTCATCTTATTTTGTATATACTACCTAGCCCAACAATGTAGAGGTTAACGAAAAATGCGCTCACCAATTTGTCATCTTTTCTCAGCAATTAATTCATCACCATTTAAGATTGCACCAGAGAAGGAGCAAGATCTTaa                                                                                                               <  2:4646391/150‑1 (MQ=255)
                                      aaaaaaCTGAAGATTCATCTTATTTTGTATATACTACCTAGCCCAACAATGTAGAGGTTAACGAAAAATGCGCTCACCAATTTGTCATCTTTTCTCAGCAATTAATTCATCACCATTTAAGATTGCACCAGAGAAGGAGCAAGATCTTaa                                                                                                               <  1:3137750/150‑1 (MQ=255)
                                      aaaaaaCTGAAGATTCATCTTATTTTGTATATACTACCTAGCCCAACAATGTAGAGGTTAACGAAAAATGCGCTCACCAATTTGTCATCTTTTCTCAGCAATTAATTCATCACCATTTAAGATTGCACCAGAGAAGGAGCAAGATCTTaa                                                                                                               <  1:3137868/150‑1 (MQ=255)
                                      aaaaaaCTGAAGATTCATCTTATTTTGTATATACTACCTAGCCCAACAATGTAGAGGTTAACGAAAAATGCGCTCACCAATTTGTCATCTTTTCTCAGCAATTAATTCATAACCATTTAAGATTGCACCAGAGAAGGAGCAAGATCTTaa                                                                                                               >  1:2469339/1‑150 (MQ=255)
                                      aaaaaaCTGAAGAGTCATCTTATTGTGTATATACTACCTAGCCCAACAATGTAGAGGTTAACGAAAAATGCGCTCACCAATTTGTCATCTTTTCTCAGCAATTAATTCATCACCATTTAAGATTGCACCAGAGAAGGAGCAAGATCTTaa                                                                                                               <  2:2688595/150‑1 (MQ=255)
                                       aaaaaCTGAAGATTCATCTTATTTTGTATATACTACCTAGCCCAACAATGTAGAGGTTAACGAAAAATGCGCTCACCAATTTGTCATCTTTTCTCAGCAATTAATTCATCACCATTTAAGATTGCACCAGAGAAGGAGCAAGATCTTaaa                                                                                                              >  2:1188247/1‑150 (MQ=255)
                                        aaaaCTGAAGATTCATCTTATTTTGTATATACTACCTAGCCCAACAATGTAGAGGTTAACGAAAAATGCGCTCACCAATTTGTCATCTTTTCTCAGCAATTAATTCATCACCATTTAAGATTGCACCAGAGAAGGAGCAAGATCTTaaaa                                                                                                             >  1:578790/1‑150 (MQ=255)
                                         aaaCTGAAGATTCATCTTATTTTGTATATACTACCTAGCCCAACAATGTAGAGGTTAACGAAAAATGCGCTCACCAATTTGTCATCTTTTCTCAGCAATTAATTCATCACCATTTAAGATTGCACCAGAGAAGGAGCAAGATCTTAAAAc                                                                                                            >  1:1685143/1‑150 (MQ=255)
                                          aaCTGAAGATTCATCTTATTTTGTATATACTACCTAGCCCAACAATGTAGAGGTTAACGAAAAATGCGCTCACCAATTTGTCATCTTTTCTCAGCAATTAATTCATCACCATTTAAGATTGCACCAGAGAAGGAGCAAGATCTTAAAACg                                                                                                           >  2:4414766/1‑150 (MQ=255)
                                           aCTGAAGATTCATCTTATTTTGTATATACTACCTAGCCCAACAATGTAGAGGTTAACGAAAAATGCGCTCACCAATTTGTCATCTTTTCTCAGCAATTAATTCATAACCATTTAAGATTGCACCAGAGAAGGAGCAAGATCTTAAAACGa                                                                                                          >  1:915574/1‑150 (MQ=255)
                                           aCTGAAGATTCATCTTATTTTGTATATACTACCTAGCCCAACAATGTAGAGGTTAACGAAAAATGCGCTCACCAATTTGTCATCTTTTCTCAGCAATTAATTCATAACCATTTAAGATTGCACCAGAGAAGGAGCAAGATCTTAAAACGa                                                                                                          >  1:915563/1‑150 (MQ=255)
                                           aCTGAAGATTCATCTTATTTTGTATATACTACCTAGCCCAACAATGTAGAGGTTAACGAAAAATGCGCTCACCAATTTGTCATCTTTTCTCAGCAATTAATTCATAACCATTTAAGATTGCACCAGAGAAGGAGCAAGATCTTAAAACGa                                                                                                          >  1:645280/1‑150 (MQ=255)
                                             tGAAGATTCATCTTATTTTGTATATACTACCTAGCCCAACAATGTAGAGGTTAACGAAAAATGCGCTCACCAATTTGTCATCTTTTCTCAGCAATTAATTCATCACCATTTAAGATTGCACCAGAGAAGGAGCAAGATCTTAAAACGATa                                                                                                        <  1:2457746/150‑1 (MQ=255)
                                             tGAAGATTCATCTTATTTTGTATAGACTACCTAGCCCAACAATGTAGAGGTTAACGAAAAATGCGCTCACCAATTTGTCATCTTTTCTCAGCAATTAATTCATCACCATTTAAGATTGCACCAGAGAAGGAGCAAGATCTTAAAACGATa                                                                                                        <  2:3538129/150‑1 (MQ=255)
                                               aaGATTCATCTTATTTTGTATATACTACCTAGCCCAACAATGTAGAGGTTAACGAAAAATGCGCTCACCAATTTGTCATCTTTTCTCAGCAATTAATTCATCACCATTTAAGATTGCACCAGAGAAGGAGCAAGATCTTAAAACGATAGt                                                                                                      >  1:1237881/1‑150 (MQ=255)
                                                 gATTCATCTTATTTTGTATATACTACCTAGCCCAACAATGTAGAGGTTAACGAAAAATGCGCTCACCAATTTGTCATCTTTTCTCAGCAATTAATTCATAACCATTTAAGATTGCACCAGAGAAGGAGCAAGATCTTAAAACGATAGTTg                                                                                                    <  1:3379449/150‑1 (MQ=255)
                                                  aTTCATCTTATTTTGTATATACTACCTAGCCCAACAATGTAGAGGTTAACGAAAAATGCGCTCACCAATTTGTCATCTTTTCTCAGCAATTAATTCATCACCATTTAAGATTGCACCAGAGAAGGAGCAAGATCTTAAAACGATAGTTga                                                                                                   >  1:3251903/1‑150 (MQ=255)
                                                    tCATCTTATTTTGTATATACTACCTAGCCCAACAATGTAGAGGTTAACGAAAAATGCGCTCACCAATTTGTCATCTTTTCTCAGCAATTAATTCATCACCATTTAAGATTGCACCAGAGAAGGAGCAAGATCTTAAAACGATAGTTgacg                                                                                                 <  1:2313292/150‑1 (MQ=255)
                                                    tCATCTTATTTTGTATATACTACCTAGCCCAACAATGTAGAGGTTAACGAAAAATGCGCTCACCAATTTGTCATCTTTTCTCAGCAATTAATTCATAACCATTTAAGATTGCACCAGAGAAGGAGCAAGATCTTAAAACGATAGTTgacg                                                                                                 <  2:3936607/150‑1 (MQ=255)
                                                        cTTATTTTGTATATACTACCTAGCCCAACAATGTAGAGGTTAACGAAAAATGCGCTCACCAATTTGTCATCTTTTCTCAGCAATTAATTCATCACCATTTAAGATTGCACCAGAGAAGGAGCAAGATCTTAAAACGATAGTTGACGACaa                                                                                             <  2:2663997/150‑1 (MQ=255)
                                                        cTTATTTTGTATATACTACCTAGCCCAACAATGTAGAGGTTAACGAAAAATGCGCTCACCAATTTGTCATCTTTTCTCAGCAATTAATTCATCACCATTTAAGATTGCACCAGAGAAGGAGCAAGATCTTAAAACGATAGTTGACGACaa                                                                                             <  2:2663754/150‑1 (MQ=255)
                                                        cTTATTTTGTATATACTACCTAGCCCAACAATGTAGAGGTTAACGAAAAATGCGCTCACCAATTTGTCATCTTTTCTCAGCAATTAATTCATCACCATTTAAGATTGCACCAGAGAAGGAGCAAGATCTTAAAACGATAGTTGACGACaa                                                                                             <  1:3036778/150‑1 (MQ=255)
                                                        cTTATTTTGTATATACTACCTAGCCCAACAATGTAGAGGTTAACGAAAAATGCGCTCACCAATTTGTCATCTTTTCTCAGCAATTAATTCATCACCATTTAAGATTGCACCAGAGAAGGAGCAAGATCTTAAAACGATAGTTGACGACaa                                                                                             <  1:3036777/150‑1 (MQ=255)
                                                        cTTATTTTGTATATACTACCTAGCCCAACAATGTAGAGGTTAACGAAAAATGCGCTCACCAATTTGTCATCTTTTCTCAGCAATTAATTCATCACCATTTAAGATTGCACCAGAGAAGGAGCAAGATCTTAAAACGATAGTTGACGACaa                                                                                             >  2:1331099/1‑150 (MQ=255)
                                                          tATTTTGTATATACTACCTAGCCCAACAATGTAGAGGTTAACGAAAAATGCGCTCACCAATTTGTCATCTTTTCTCAGCAATTAATTCATAACCATTTAAGATTGCACCAGAGAAGGAGCAAGATCTTAAAACGATAGTTGACGACaaaa                                                                                           <  2:779222/150‑1 (MQ=255)
                                                            ttttGTATATACTACCTAGCCCAACAATGTAGAGGTTAACGAAAAATGCGCTCACCAATTTGTCATCTTTTCTCAGCAATTAATTCATCACCATTTAAGATTGCACCAGAGAAGGAGCAAGATCTTAAAACGATAGTTGACGACaaaaaa                                                                                         >  2:1787659/1‑150 (MQ=255)
                                                             tttGTATATACTACCTAGCCCAACAATGTAGAGGTTAACGAAAAATGCGCTCACCAATTTGTCATCTTTTCTCAGCAATTAATTCATCACCATTTAAGATTGCACCAGAGAAGGAGCAAGATCTTAAAACGATAGTTGACGACaaaaaaa                                                                                        >  2:2843318/1‑150 (MQ=255)
                                                               tGTATATACTACCTAGCCCAACAATGTAGAGGTTAACGAAAAATGCGCTCACCAATTTGTCATCTTTTCTCAGCAATTAATTCATCACCATTTAAGATTGCACCAGAGAAGGAGCAAGATCTTAAAACGATAGTTGACGACAAAAAAAtt                                                                                      >  1:4078908/1‑150 (MQ=255)
                                                                  atataCTACCTAGCCCAACAATGTAGAGGTTAACGAAAAATGCGCTCACCAATTTGTCATCTTTTCTCAGCAATTAATTCATCACCATTTAAGATTGCACCAGAGAAGGAGCAAGATCTTAAAACGATAGTTGACGACAAAAAAATtata                                                                                   >  2:480871/1‑150 (MQ=255)
                                                                  atataCTACCTAGCCCAACAATGTAGAGGTTAACGAAAAATGCGCTCACCAATTTGTCATCTTTTCTCAGCAATTAATTCATCACCATTTAAGATTGCACCAGAGAAGGAGCAAGATCTTAAAACGATAGTTGACGACAAAAAAATtata                                                                                   >  2:480616/1‑150 (MQ=255)
                                                                  atataCTACCTAGCCCAACAATGTAGAGGTTAAAGAAAATTGGTCTCACCAATTTGTCATCTTTTCTCATCAATTTATTCATCACCCTTTAAGATTGCACCAGAGACGTAGCAAGATCTTAAAACGATAGTTGACGACAAAAAATTtata                                                                                   <  1:512862/150‑1 (MQ=255)
                                                                   tataCTACCTAGCCCAACAATGTAGAGGTTAACGAAAAATGCGCTCACCAATTTGTCATCTTTTCTCAGCAATTAATTCATCACCATTTAAGATTGCACCAGAGAAGGAGCAAGATCTTAAAACGATAGTTGACGACAAAAAAATTATaa                                                                                  >  2:2818491/1‑150 (MQ=255)
                                                                   tataCTACCTAGCCCAACAATGTAGAGGTTAACGAAAAATGCGCTCACCAATTTGTCATCTTTTCTCAGCAATTAATTCATCACCATGTAAGATTGCACCAGAGACGGAGCAAGATCTTAAAACGATAGTTGACGACCAAAAAATtatac                                                                                  >  2:4035144/1‑149 (MQ=255)
                                                                      actacCTAGCCCAACAATGTAGAGGTTAACGAAAAATGCGCTCACCAATTTGTCATCTTTTCTCAGCAATTAATTCATCACCATTTAAGATTGCACCAGAGAAGGAGCAAGATCTTAAAACGATAGTTGACGACAAACAAATTATAAttt                                                                               >  2:3191793/1‑150 (MQ=255)
                                                                      actacCTAGCCCAACAATGTAGAGGTTAACGAAAAATGCGCTCACCAATGTGTCATCTTTTCTCAGCCATTAATTCATCACCATTTAAGATTGCACCAGAGAAGGAGCAAGATCTTAAAACGATCGTTGACGACAAAAAAATTATAAtgt                                                                               >  2:3306013/1‑148 (MQ=255)
                                                                       ctacCTAGCCCAACAATGTAGAGGTTAACGAAAAATGCGCTCACCAATTTGTCATCTTTTCTCAGCAATTAATTCATCACCATTTAAGATTGCACCAGAGAAGGAGCAAGATCTTAAAACGATAGTTGACGACAAAAAAATTATAATTTc                                                                              <  2:149145/150‑1 (MQ=255)
                                                                       ctacCTAGCCCAACAATGTAGAGGTTAACGAAAAATGCGCTCACCAATTTGTCATCTTTTCTCAGCAATTAATTCATCACCATTTAAGATTGCACCAGAGAAGGAGCAAGATCTTAAAACGATAGTTGACGACAAAAAAATTATAATTTc                                                                              <  1:2766623/150‑1 (MQ=255)
                                                                       ctacCTAGCCCAACAATGTAGAGGTTAACGAAAAATGCGCTCACCAATTTGTCATCTTTTCTCAGCAATTAATTCATCACCATTTAAGATTGCACCAGAGAAGGAGCAAGATCTTAAAACGATAGTTGACGACAAAAAAATTATAATTTc                                                                              >  1:1139873/1‑150 (MQ=255)
                                                                       ctacCTAGCCCAACAATGTAGAGGTTAACGAAAAATGCGCTCACCAATTTGTCATCTTTTCTCAGCAATTAATTCATCACCATTTAAGATTGCACCAGAGAAGGAGCAAGATCTTAAAACGATAGTTGACGACAAAAAAATTATAATTTc                                                                              <  2:2878766/150‑1 (MQ=255)
                                                                       ctacCTAGCCCAACAATGTAGAGGTTAACGAAAAATGCGCTCACCAATTTGTCATCTTTTCTCAGCAATTAATTCATCACCATTTAAGATTGCACCAGAGAAGGAGCAAGATCTTAAAACGATAGTTGACGACAAAAAAATTATAATTTc                                                                              <  1:1400920/150‑1 (MQ=255)
                                                                       ctacCTAGCCCAACAATGTAGAGGTTAACGAAAAATGCGCTCACCAATTTGTCATCTTTTCTCAGCAATTAATTCATCACCATTTAAGATTGCACCAGAGAAGGAGCAAGATCTTAAAACGATAGTTGACGACAAAAAAATTATAATTTc                                                                              <  2:283286/150‑1 (MQ=255)
                                                                           cTAGCCCAACAATGTAGAGGTTAACGAAAAATGCGCTCACCAATTTGTCATCTTTTCTCAGCAATTAATTCATCACCATTTAAGATTGCACCAGAGAAGGAGCAAGATCTTAAAACGATAGTTGACGACAAAAAAATTATAATTTCAGtt                                                                          >  1:2041535/1‑150 (MQ=255)
                                                                           cTAGCCCAACAATGTAGAGGTTAACGAAAAATGCGCTCACCAATTTGTCATCTTTTCTCAGCAATTAATTCATCACCATTTAAGATTGCACCAGAGAAGGAGCAAGATCTTAAAACGATAGTTGACGACAAAAAAATTATAATTTAAGtt                                                                          >  2:4632037/1‑150 (MQ=255)
                                                                            tAGCCCAACAATGTAGAGGTTAACGAAAAATGCGCTCACCAATTTGTCATCTTTTCTCAGCAATTAATTCATCACCATTTAAGATTGCACCAGAGAAGGAGCAAGATCTTAAAACGATAGTTGACGACAAAAAAATTATAATTTCAGTtg                                                                         >  2:3013204/1‑150 (MQ=255)
                                                                             aGCCCAACAATGTAGAGGTTAACGAAAAATGCGCTCACCAATTTGTCATCTTTTCTCAGCAATTAATTCATCACCATTTAAGATTGCACCAGAGAAGGAGCAAGATCTTAAAACGATAGTTGACGACAAAAAAATTATAATTTCAGTtgt                                                                        <  1:3172902/150‑1 (MQ=255)
                                                                              gCCCAACAATGTAGAGGTTAACGAAAAATGCGCTCACCAATTTGTCATCTTTTCTCAGCAATTAATTCATCACCATTTAAGATTGCACCAGAGAAGGAGCAAGATCTTAAAACGATAGTTGACGACAAAAAAATTATAATTTCAGTtgtg                                                                       <  2:3256505/150‑1 (MQ=255)
                                                                                 caacaaTGTAGAGGTTAACGAAAAATGCGCTCACCAATTTGTCATCTTTTCTCAGCAATTAATTCATCACCATTTAAGATTGCACCAGAGAAGGAGCAAGATCTTAAAACGATAGTTGACGACAAAAAAATTATAATTTCAGTTgtgagt                                                                    >  1:2369183/1‑150 (MQ=255)
                                                                                 caacaaTGTAGAGGTTAACGAAAAATGCGCTCACCAATTTGTCATCTTTTCTCAGCAATTAATTCATCACCATTTAAGATTGCACCAGAGAAGGAGCAAGATCTTAAAACGATAGTTGACGACAAAAAAATTATAATTTCAGTTgtgagt                                                                    >  1:4704646/1‑150 (MQ=255)
                                                                                   acaaTGTAGAGGTTAACGAAAAATGCGCTCACCAATTTGTCATCTTTTCTCAGCAATTAATTCATCACCATTTAAGATTGCACCAGAGAAGGAGCAAGATCTTAAAACGATAGTTGACGACAAAAAAATTATAATTTCAGTTgtgagtga                                                                  >  1:1306145/1‑150 (MQ=255)
                                                                                    caaTGTAGAGGTTAACGAAAAATGCGCTCACCAATTTGTCATCTTTTCTCAGCAATTAATTCATCACCATTTAAGATTGCACCAGAGAAGGAGCAAGATCTTAAAACGATAGTTGACGACAAAAAAATTATAATTTCAGTTGTGAGTGaa                                                                 >  1:4176130/1‑150 (MQ=255)
                                                                                      aTGTAGAGGTTAACGAAAAATGCGCTCACCAATTTGTCATCTTTTCTCAGCAATTAATTCATCACCATTTAAGATTGCACCAGAGAAGGAGCAAGATCTTAAAACGATAGTTGACGACAAAAAAATTATAATTTCAGTTGTGAGTGAAcc                                                               <  1:493051/150‑1 (MQ=255)
                                                                                            agGTTAAAGAAAAATGCGCTCAACAATTTGTCATCTTTTCTCATCAATTAATTCATCACCATTTAAGATTGCACCAGAGAAGGAGCAAGATCTTAAAAAGATAGTTGACGACAAAAAAATTATAATTTCAGTTGTGAGTGAACCTGGttt                                                         <  1:2095752/150‑1 (MQ=255)
                                                                                             ggTTAACGAAAAATGCGCTCACCAATTTGTCATCTTTTCTCAGCAATTAATTCATCACCATTTAAGATTGCACCAGAGAAGGAGCAAGATCTTAAAACGATAGTTGACGACAAAAAAATTATAATTTCAGTTGTGAGTGAACCTGGtttt                                                        <  2:3246474/150‑1 (MQ=255)
                                                                                                tAACGAAAAATGCGCTCACCAATTTGTCATCTTTTCTCAGCAATTAATTCATAACCATTTAAGATTGCACCAGAGAAGGAGCAAGATCTTAAAACGATAGTTGACGACAAAAAAATTATAATTTCAGTTGTGAGTGAACCTGGTTTTAat                                                     >  2:1743396/1‑150 (MQ=255)
                                                                                                   cGAAAAATGCGCTCACCAATTTGTCATCTTTTCTCAGCAATTAATTCATCACCATTTAAGATTGCACCAGAGAAGGAGCAAGATCTTAAAACGATAGTTGACGACAAAAAAATGATAATTTCAGTTGTGAGTGAACCGGGTTTTAATATc                                                  >  2:513397/1‑150 (MQ=255)
                                                                                                          tGCGCTCACCAATTTGTCATCTTTTCTCAGCAATTAATTCATCACCATTTAAGATTGCACCAGAGAAGGAGCAAGATCTTAAAACGATAGTTGACGACAAAAAAATTATAATTTCAGTTGTGAGTGAACCTGGTTTTAATATCCGAGTCa                                           <  1:3554997/150‑1 (MQ=255)
                                                                                                               tCACCAATTTGTCATCTTTTCTCAGCAATTAATTCATCACCATTTAAGATTGCACCAGAGAAGGAGCAAGATCTTAAAACGATAGTTGACGACAAAAAAATTATAATTTCAGTTGTGAGTGAACCTGGTTTTAATATCCGAGTCAGgaag                                      <  2:4052458/150‑1 (MQ=255)
                                                                                                               tCACCAATTTGTCATCTTTTCTCAGCAATTAATTCATCACCATTTAAGATTGCACCAGAGAAGGAGCAAGATCTTAAAACGATAGTTGACGACAAAAAAATTATAATTTCAGTTGTGAGTGAACCTGGTTTTAATATCCGAGTCAGgaag                                      <  2:1376024/150‑1 (MQ=255)
                                                                                                                cACCAATTTGTCATCTTTTCTCAGCAATTAATTCATCACCATTTAAGATTGCACCAGAGAAGGAGCAAGATCTTAAAACGATAGTTGACGACAAAAAAATTATAATTTCAGTTGTGAGTGAACCTGGTTTTAATATCCGAGTCAGgaaga                                     <  1:334129/150‑1 (MQ=255)
                                                                                                                  ccAATTTGTCATCTTTTCTCAGCAATTAATTCATCACCATTTAAGATTGCACCAGAGAAGGAGCAAGATCTTAAAACGATAGTTGACGACAAAAAAATTATAATTTCAGTTGTGAGTGAACCTGGTTTTAATATCCGAGTCAGGAAGAAt                                   <  1:3459476/150‑1 (MQ=255)
                                                                                                                        tGTCATCTTTTCTCAGCAATTAATTCATCACCATTTAAGATTGCACCAGAGAAGGAGCAAGATCTTAAAACGATAGTTGACGACAAAAAAATTATAATTTCAGTTGTGAGTGAACCTGGTTTTAATATCCGAGTCAGGAAGAATGAGAGt                             <  1:1786599/150‑1 (MQ=255)
                                                                                                                        tGTCATCTTTTCTCAGCAATTAATTCATAACCATTTAAGATTGCACCAGAGAAGGAGCAAGATCTTAAAACGATAGTTGACGACAAAAAAATTATAATTTCAGTTGTGAGTGAACCTGGTTTTAATATCCGAGTCAGGAAGAATGAGAGt                             >  1:1155259/1‑150 (MQ=255)
                                                                                                                         gTCATCTTTTCTCAGCAATTAATTCATCACCATTTAAGATTGCACCAGAGAAGGAGCAAGATCTTAAAACGATAGTTGACGACAAAAAAATTATAATTTCAGTTGTGAGTGAACCTGGTTTTAATATCCGAGTCAGGAAGAATGAGAGta                            <  2:318857/150‑1 (MQ=255)
                                                                                                                             tCTTTTCTCAGCAATTAATTCATCACCATTTAAGATTGCACCAGAGAAGGAGCAAGATCTTAAAACGATAGTTGACGACAAAAAAATTATAATTTCAGTTGTGAGTGAACCTGGTTTTAATATCCGAGTCAGGAAGAATGAGAGtaataa                        <  1:1282071/150‑1 (MQ=255)
                                                                                                                                tttCTCAGCAATTAATTCATCACCATTTAAGATTGCACCAGAGAAGGAGCAAGATCTTAAAACGATAGTTGACGACAAAAAAATTATAATTTCAGTTGTGAGTGAACCTGGTTTTAATATCCGAGTCAGGAAGAATGAGAGTAATAATTc                     <  1:2132644/150‑1 (MQ=255)
                                                                                                                                 ttCTCAGCAATTAATTCATCACCATTTAAGATTGCACCAGAGAAGGAGCAAGATCTTAAAACGATAGTTGACGACAAAAAAATTATAATTTCAGTTGTGAGTGAACCTGGTTTTAATATCCGAGTCAGGAAGAATGAGAGTAATAATTca                    <  1:1923277/150‑1 (MQ=255)
                                                                                                                                 ttCTCAGCAATTAATTCATCACCATTTAAGATTGCACCAGAGAAGGAGCAAGATCTTAAAACGATAGTTGACGACAAAAAAATTATAATTTCAGTTGTGAGTGAACCTGGTTTTAATATCCGAGTCAGGAAGAATGAGAGTAATAATTca                    <  1:2581202/150‑1 (MQ=255)
                                                                                                                                      aGCAATTAATTCATCACCATTTAAGATTGCACCAGAGAAGGAGCAAGATCTTAAAACGATAGTTGACGACAAAAAAATTATAATTTCAGTTGTGAGTGAACCTGGTTTTAATATCCGAGTCAGGAAGAATGAGAGTAATAATTCACATGa               <  2:3350321/150‑1 (MQ=255)
                                                                                                                                        cAATTAATTCATCACCATTTAAGATTGCACCAGAGAAGGAGCAAGATCTTAAAACGATAGTTGACGACAAAAAAATTATAATTTCAGTTGTGAGTGAACCTGGTTTTAATATCCGAGTCAGGAAGAATGAGAGTAATAATTCACATGaaa             >  2:2406209/1‑150 (MQ=255)
                                                                                                                                            taattCATCACCATTTAAGATTGCACCAGAGAAGGAGCAAGATCTTAAAACGATAGTTGACGACAAAAAAATTATAATTTCAGTTGTGAGTGAACCTGGTTTTAATATCCGAGTCAGGAAGAATGAGAGTAATAATTCACATGAAATAGt         >  1:1982565/1‑150 (MQ=255)
                                                                                                                                             aattCATAACCATTTAAGATTGCACCAGAGAAGGAGCAAGATCTTAAAACGATAGTTGATGACAAAAAAATTATAATTTCAGTTGTGAGTGAACCTGGTTTTAATATCCGAGTCAGGAAGAATGAGAGTAATAATTCACATGAAATAGtt        >  1:133032/1‑150 (MQ=255)
                                                                                                                                               ttCATCACCATTTAAGATTGCACCAGAGAAGGAGCAAGATCTTAAAACGATAGTTGACGACAAAAAAATTATAATTTCAGTTGTGAGTGAACCTGGTTTTAATATCCGAGTCAGGAAGAATGAGAGTAATAATTCACATGAAATAGTTCt      <  1:4486544/150‑1 (MQ=255)
                                                                                                                                                tcatcaCCATTTAAGATTGCACCAGAGAAGGAGCAAGATCTTAAAACGATAGTTGACGACAAAAAAATTATAATTTCAGTTGTGAGTGAACCTGGTTTTAATATCCGAGTCAGGAAGAATGAGAGTAATAATTCACATGAAATAGTTCTa     >  2:254790/1‑150 (MQ=255)
                                                                                                                                                  atcaCCATTTAAGATTGCACCAGAGAAGGAGCAAGATCTTAAAACGATAGTTGACGACAAAAAAATTATAATTTCAGTTGTGAGTGAACCTGGTTTTAATATCCGAGTCAGGAAGAATGAGAGTAATAATTCACATGAAATAGTTCTAAc   <  2:1043781/150‑1 (MQ=255)
                                                                                                                                                   tcaCCATTTAAGATTGCACCAGAGAAGGAGCAAGATCTTAAAACGATAGTTGACGACAAAAAAATTATAATTTCAGTTGTGAGTGAACCTGGTTTTAATATCCGAGTCAGGAAGAATGAGAGTAATAATTCACATGAAATAGTTCTAACa  <  2:3245838/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
ATGTCGATAATTCATTACGCATATAGTATCGAACATAGAAAAAACTGAAGATTCATCTTATTTTGTATATACTACCTAGCCCAACAATGTAGAGGTTAACGAAAAATGCGCTCACCAATTTGTCATCTTTTCTCAGCAATTAATTCATCACCATTTAAGATTGCACCAGAGAAGGAGCAAGATCTTAAAACGATAGTTGACGACAAAAAAATTATAATTTCAGTTGTGAGTGAACCTGGTTTTAATATCCGAGTCAGGAAGAATGAGAGTAATAATTCACATGAAATAGTTCTAACA  >  CP009273/1194046‑1194342

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑