Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,519,349 T→A 100% K297* (AAA→TAA)  ansP ← L‑asparagine transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,519,3490TA95.7% 973.1 / 21.8 276K297* (AAA→TAA) ansPL‑asparagine transporter
Reads supporting (aligned to +/- strand):  ref base T (2/10);  new base A (123/141);  total (125/151)
Fisher's exact test for biased strand distribution p-value = 7.13e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 277 total aligned reads displayed.

TTCGCCATAAAACTCGGTGCGGAACCGCCCATCGCCATTGAGCGCAGAATACGTCCGGTGCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTTAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAATCACACTGTTAATGGCTTTAGGCACCATGGTCTGCGGATC  >  CP009273/1519204‑1519496
                                                                                                                                                 |                                                                                                                                                   
ttCGCCATAAAACTCGGTGCGGAACCGCCCATCGCCATTGAGCGCAGAATACGTCCGGTGCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAagag                                                                                                                                                 >  1:3392593/1‑150 (MQ=255)
    ccATAAAACTCGGTGCGGAACCGCCCATCGCCATTGAGCGCAGAATACGTCCGGTGCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGaaaa                                                                                                                                             >  1:3496598/1‑150 (MQ=255)
     cATAAAACTCGGTGCGGAACCGCCCATCGCCATTGAGCGCAGAATACGTCCGGTGCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGaaaaa                                                                                                                                            <  1:2015835/150‑1 (MQ=255)
      aTAAAACTCGGTGCGGAACCGCCCATCGCCATTGAGCGCAGAATACGTCCGGTGCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAAc                                                                                                                                           <  1:3767471/150‑1 (MQ=255)
        aaaaCTCGGTGCGGAACCGCCCATCGCCATTGAGCGCAGAATACGTCCGGTGCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGt                                                                                                                                         >  2:3064415/1‑150 (MQ=255)
            cTCGGTGCGGAACCGCCCATCGCCATTGAGCGCAGAATACGTCCGGTGCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACg                                                                                                                                     >  1:1790094/1‑150 (MQ=255)
             tCGGTGCGGAACCGCCCATCGCCATTGAGCGCAGAATACGTCCGGTGCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGa                                                                                                                                    >  2:5140571/1‑150 (MQ=255)
               ggTGCGGAACCGCCCATCGCCATTGAGCGCAGAATACGTCCGGTGCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAAc                                                                                                                                  >  2:2705478/1‑150 (MQ=255)
                gTGCGGAACCGCCCATCGCCATTGAGCGCAGAATACGTCCGGTGCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACg                                                                                                                                 <  1:2890948/150‑1 (MQ=255)
                gTGCGGAACCGCCCATCGCCATTGAGCGCAGAATACGTCCGGTGCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACg                                                                                                                                 <  2:3991507/150‑1 (MQ=255)
                    ggAACCGCCCATCGCCATTGAGCGCAGAATACGTCCGGTGCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACt                                                                                                                             >  2:1290492/1‑150 (MQ=255)
                       aCCGCCCATCGCCATTGAGCGCAGAATACGTCCGGTGCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTg                                                                                                                          >  2:4322300/1‑150 (MQ=255)
                         cGCCCATCGCCATTGAGCGCAGAATACGTCCGGTGCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGcc                                                                                                                        <  1:3882243/150‑1 (MQ=255)
                           cccATCGCCATTGAGCGCAGAATACGTCCGGTGCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGcccc                                                                                                                      >  2:440534/1‑150 (MQ=255)
                            ccATCGCCATTGAGCGCAGAATACGTCCGGTGCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCg                                                                                                                     <  2:1404474/150‑1 (MQ=255)
                               tCGCCATTGAGCGCAGAATACGTCCGGTGCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCt                                                                                                                  >  1:4220760/1‑150 (MQ=255)
                               tCGCCATTGAGCGCAGAATACGTCCGGTGCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCt                                                                                                                  <  1:4359978/150‑1 (MQ=255)
                               tCGCCATTGAGCGCAGAATACGTCCGGTGCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCt                                                                                                                  >  2:4618086/1‑150 (MQ=255)
                                cGCCATTGAGCGCAGAATACGTCCGGTGCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTg                                                                                                                 >  1:4121602/1‑150 (MQ=255)
                                   cATTGAGCGCAGAATACGTCCGGTGCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATa                                                                                                              <  1:2923235/150‑1 (MQ=255)
                                    aTTGAGCGCAGAATACGTCCGGTGCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATAc                                                                                                             >  1:4287571/1‑150 (MQ=255)
                                      tGAGCGCAGAATACGTCCGGTGCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTTAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATAcgc                                                                                                           <  1:4017594/150‑1 (MQ=255)
                                       gAGCGCAGAATACGTCCGGTGCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTTAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATAcgcg                                                                                                          <  1:4251238/150‑1 (MQ=255)
                                       gAGCGCAGAATACGTCCGGTGCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATAcgcg                                                                                                          <  2:2897607/150‑1 (MQ=255)
                                       gAGCGCAGAATACGTCCGGTGCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATAcgcg                                                                                                          <  2:3018284/150‑1 (MQ=255)
                                       gAGCGCAGAATACGTCCGGTGCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATAcgcg                                                                                                          <  1:5054118/150‑1 (MQ=255)
                                         gcgcAGAATACGTCCGGTGCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCt                                                                                                        <  1:897447/150‑1 (MQ=255)
                                           gcAGAATACGTCCGGTGCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTcc                                                                                                      <  2:110651/150‑1 (MQ=255)
                                              gAATACGTCCGGTGCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCTGCCTGATACGCGCTCCACg                                                                                                   <  1:3044388/150‑1 (MQ=255)
                                                aTACGTCCGGTGCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGc                                                                                                 <  2:901367/150‑1 (MQ=255)
                                                aTACGTCCGGTGCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGc                                                                                                 >  2:3618709/1‑150 (MQ=255)
                                                   cGTCCGGTGCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAat                                                                                              <  2:1037333/150‑1 (MQ=255)
                                                    gTCCGGTGCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTTAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAata                                                                                             >  1:1149110/1‑150 (MQ=255)
                                                      ccGGTGCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAataac                                                                                           <  2:2024725/150‑1 (MQ=255)
                                                       cGGTGCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACTTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAataaca                                                                                          >  1:3539577/1‑150 (MQ=255)
                                                        ggTGCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTTAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAataacat                                                                                         <  2:3764914/150‑1 (MQ=255)
                                                        ggTGCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTTAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAataacat                                                                                         <  2:3765124/150‑1 (MQ=255)
                                                        ggTGCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAataacat                                                                                         <  1:1194072/150‑1 (MQ=255)
                                                         gTGCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAataacata                                                                                        <  2:1853157/150‑1 (MQ=255)
                                                          tGCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAataacataa                                                                                       <  1:3921080/150‑1 (MQ=255)
                                                          tGCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAataacataa                                                                                       <  1:633947/150‑1 (MQ=255)
                                                           gCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAataacataac                                                                                      >  2:1323075/1‑150 (MQ=255)
                                                             aGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCa                                                                                    >  1:2588321/1‑150 (MQ=255)
                                                               tACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGc                                                                                  <  1:860915/150‑1 (MQ=255)
                                                                aCAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCa                                                                                 <  1:739089/150‑1 (MQ=255)
                                                                 cAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCaa                                                                                >  2:3331031/1‑150 (MQ=255)
                                                                 cAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCaa                                                                                >  1:3326944/1‑150 (MQ=255)
                                                                  aGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAac                                                                               >  1:2336950/1‑150 (MQ=255)
                                                                  aGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAac                                                                               <  2:618338/150‑1 (MQ=255)
                                                                    aCCTGAAGTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAacac                                                                             <  2:4040811/150‑1 (MQ=255)
                                                                     ccTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAAcacc                                                                            >  1:375296/1‑150 (MQ=255)
                                                                       tGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAAcaccac                                                                          <  1:5104412/150‑1 (MQ=255)
                                                                       tGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAAcaccac                                                                          <  1:4827661/150‑1 (MQ=255)
                                                                       tGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAAcaccac                                                                          >  1:643698/1‑150 (MQ=255)
                                                                          aTTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGa                                                                       <  2:1664383/150‑1 (MQ=255)
                                                                          aTTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGa                                                                       >  2:4505748/1‑150 (MQ=255)
                                                                            tCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGc                                                                     >  1:2674669/1‑150 (MQ=255)
                                                                             cAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATACTGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGcc                                                                    <  2:3446327/150‑1 (MQ=255)
                                                                             cAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGcc                                                                    <  1:1643405/150‑1 (MQ=255)
                                                                              aGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCg                                                                   <  1:1377900/150‑1 (MQ=255)
                                                                                     agagGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTaaa                                                            <  2:397198/150‑1 (MQ=255)
                                                                                      gagGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTaaaa                                                           >  1:282085/1‑150 (MQ=255)
                                                                                        gggCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACa                                                         <  2:4229937/150‑1 (MQ=255)
                                                                                         ggCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAg                                                        >  2:638627/1‑150 (MQ=255)
                                                                                           cAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGc                                                      <  1:959747/150‑1 (MQ=255)
                                                                                            aGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGcc                                                     >  1:4905331/1‑150 (MQ=255)
                                                                                               ggTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAAt                                                  >  1:4177838/1‑150 (MQ=255)
                                                                                                 tCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATAc                                                <  2:208686/150‑1 (MQ=255)
                                                                                                 tCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATAc                                                <  2:2804620/150‑1 (MQ=255)
                                                                                                   aGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGc                                              <  2:2205077/150‑1 (MQ=255)
                                                                                                      accacAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCaa                                           <  1:306695/150‑1 (MQ=255)
                                                                                                          caATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAATca                                       <  2:4991846/150‑1 (MQ=255)
                                                                                                            aTGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAATcaca                                     >  2:1240213/1‑150 (MQ=255)
                                                                                                            aTGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAATaaca                                     >  1:3577517/1‑150 (MQ=255)
                                                                                                              gTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAATCACACt                                   <  2:1596274/150‑1 (MQ=255)
                                                                                                              gTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAATCACACt                                   >  1:380258/1‑150 (MQ=255)
                                                                                                               ttCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAATCACACTg                                  <  2:2163573/150‑1 (MQ=255)
                                                                                                                tCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAATCACACTGt                                 >  2:1936265/1‑150 (MQ=255)
                                                                                                                tCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAATCACACTGt                                 >  2:3576513/1‑150 (MQ=255)
                                                                                                                 cATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAATCACACTGtt                                >  1:1711177/1‑150 (MQ=255)
                                                                                                                    aaTGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAATCACACTGTTAAt                             <  1:519810/150‑1 (MQ=255)
                                                                                                                     aTGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAATCACACTGTTAATg                            <  2:4743445/150‑1 (MQ=255)
                                                                                                                     aTGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAATCACACTGTTAATg                            >  2:2633320/1‑150 (MQ=255)
                                                                                                                         tgcCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAATAACACTGTTAATGGCtt                        <  1:2581922/150‑1 (MQ=255)
                                                                                                                           ccGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAATCACACTGTTAATGGCTTTa                      <  1:1063880/150‑1 (MQ=255)
                                                                                                                            cGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAATCACACTGTTAATGGCTTTAg                     <  2:240504/150‑1 (MQ=255)
                                                                                                                             gATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAATCACACTGTTAATGGCTTTAgg                    >  1:1116924/1‑150 (MQ=255)
                                                                                                                             gATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAATCACACTGTTAATGGCTTTAgg                    <  2:322565/150‑1 (MQ=255)
                                                                                                                              atatatGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAATCACACTGTTAATGGCTTTAGGc                   <  2:4651874/150‑1 (MQ=255)
                                                                                                                                 tatGGCACACCCAGTTTAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGAAATAACATAACCAGCACCACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAATCACACTGTTAATGGCTTTAGGCAcc                <  1:3660532/150‑1 (MQ=255)
                                                                                                                                    ggCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAATCACACTGTTAATGGCTTTAGGCACCATg             >  1:1434602/1‑150 (MQ=255)
                                                                                                                                       acacCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAATCACACTGTTAATGGCTTTAGGCACCATGGTc          >  1:2395054/1‑150 (MQ=255)
                                                                                                                                       acacCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAATCACACTGTTAATGGCTTTAGGCACCATGGTc          <  1:2944853/150‑1 (MQ=255)
                                                                                                                                       acacCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAATCACACTGTTAATGGCTTTAGGCACCATGGTc          <  1:3263948/150‑1 (MQ=255)
                                                                                                                                        cacCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAATCACACTGTTAATGGCTTTAGGCACCATGGTCt         <  2:697563/150‑1 (MQ=255)
                                                                                                                                        cacCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAATCACACTGTTAATGGCTTTAGGCACCATGGTCt         <  1:4086612/150‑1 (MQ=255)
                                                                                                                                           ccAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAATCACACTGTTAATGGCTTTAGGCACCATGGTCTGCg      <  1:2189623/150‑1 (MQ=255)
                                                                                                                                           ccAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAATCACACTGTTAATGGCTTTAGGCACCATGGTCTGCg      <  2:2444924/150‑1 (MQ=255)
                                                                                                                                             aGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAATCACACTGTTAATGGCTTTAGGCACCATGGTCTGCGGa    <  2:1541010/150‑1 (MQ=255)
                                                                                                                                               tttAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAATCACACTGTTAATGGCTTTAGGCACCATGGTCTGCGGATc  <  1:2865722/150‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                   
TTCGCCATAAAACTCGGTGCGGAACCGCCCATCGCCATTGAGCGCAGAATACGTCCGGTGCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTTAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAATCACACTGTTAATGGCTTTAGGCACCATGGTCTGCGGATC  >  CP009273/1519204‑1519496

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑