Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,529,506 C→T 25.9% G204S (GGC→AGC)  yddE ← PhzC‑PhzF family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,529,5060CT25.9% 587.3 / 233.8 289G204S (GGC→AGC) yddEPhzC‑PhzF family protein
Reads supporting (aligned to +/- strand):  ref base C (99/114);  new base T (34/40);  total (134/155)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 292 total aligned reads displayed.

GCCTTTAACACGCAACACGTTGCCATCGTGGGGCAATACGTTGTGATGTACCAACCATGCACCCATCGGGCCATTGGCATTGCCAGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGGGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAGTGTCCTGTTGTTGCCACCTGAA  >  CP009273/1529357‑1529655
                                                                                                                                                     |                                                                                                                                                     
  cTTTAACACGCAACACGTTGCCATCGTGGGGCAATACGTTGTGATGTACCAACCATGCACCCATCGGGCCATTGGCATTGCCAGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTg                                                                                                                                                     >  2:5061945/1‑150 (MQ=255)
  cTTTAACACGCAACACGTTGCCATCGTGGGGCAATACGTTGTGATGTACCAACCATGCACCCATCGGGCCATTGGCATTGCCAGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTg                                                                                                                                                     >  1:5053570/1‑150 (MQ=255)
  cTTTAACACGCAACACGTTGCCATCGTGGGGCAATACGTTGTGATGTACCAACCATGCACCCATCGGGCCATTGGCATTGCCAGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTg                                                                                                                                                     >  1:800385/1‑150 (MQ=255)
  cTTTAACACGCAACACGTTGCCATCGTGGGGCAATACGTTGTGATGTACCAACCATGCACCCATCGGGCCATTGGCATTGCCAGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTg                                                                                                                                                     <  2:3884184/150‑1 (MQ=255)
     tAACACGCAACACGTTGCCATCGTGGGGCAATACGTTGTGATGTACCAACCATGCACCCATCGGGCCATTGGCATTGCCAGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGc                                                                                                                                                  >  2:1622999/1‑150 (MQ=255)
         acGCTACAATTTGACATCGTGGGTCAATACGTTGTGATGTACCAACCATGCACCCATCGGGCCATTGGCATTGACAGTCACCGGATCTTCCACAATACCAATAGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGAt                                                                                                                                              <  1:2134286/150‑1 (MQ=255)
         acGCAACACGTTGCCATCGTGGGGCAATACGTTGTGGTGTACCAACCATGCACCCATCGGGCCATTGGCATTGCCAGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGAt                                                                                                                                              <  2:2276271/150‑1 (MQ=255)
         acGCAACACGTTGCCATCGTGGGGCAATACGTTGTGATGTACCAACCATGCACCCATCGGGCCATTGGCATTGCCAGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGAt                                                                                                                                              >  2:4479536/1‑150 (MQ=255)
           gCAACACGTTGCCATCGTGGGGCAATACGTTGTGATGTACCAACCATGCACCCATCGGGCCATTGGCATTGCCAGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCTTGGGCGGATCt                                                                                                                                            <  2:2765254/150‑1 (MQ=255)
            cAACACGTTGCCATCGTGGGGCAATACGTTGTGATGTACCAACCATGCACCCATCGGGCCATTGGCATTGCCAGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGGTTTTGCTTGGGCGGATCTg                                                                                                                                           >  2:2979786/1‑150 (MQ=255)
              acacGTTGCCATCGTGGGGCAATACGTTGTGATGTACCAACCATGCACCCATCGGGCCATTGGCATTGCCAGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCTTGGGCGGATCTGGa                                                                                                                                         >  1:1064107/1‑150 (MQ=255)
              acacGTTGCCATCGTGGGGCAATACGTTGTGATGTACCAACCATGCACCCATCGGGCCATTGGCATTGCCAGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCTTGGGCGGATCTGGa                                                                                                                                         <  1:4651284/150‑1 (MQ=255)
              acacGTTGCCATCGTGGGGCAATACGTTGTGATGTACCAACCATGCACCCATCGGGCCATTGGCATTGCCAGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGa                                                                                                                                         <  2:523581/150‑1 (MQ=255)
                 cGTTGCCATCGTGGGGCAATACGTTGTGATGTACCAACCATGCACCCATCGGGCCATTGGCATTGCCAGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACg                                                                                                                                      >  1:126616/1‑150 (MQ=255)
                 cGTTGCCATCGTGGGGCAATACGTTGTGATGTACCAACCATGCACCCATCGGGCCATTGGCATTGCCAGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACg                                                                                                                                      >  2:5123517/1‑150 (MQ=255)
                    tGCCATCGTGGGGCAATACGTTGTGATGTACCAACCATGCACCCATCGGGCCATTGGCATTGCCAGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCTTGGGCGGATCTGGAACGGga                                                                                                                                   <  1:4258106/150‑1 (MQ=255)
                        aTCGTGGGGCAATACGTTGTGATGTACCAACCATGCACCCATCGGGCCATTGGCATTGCCAGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGgaagaa                                                                                                                               >  1:4934043/1‑150 (MQ=255)
                          cGTGGGGCAATACGTTGTGATGTACCAACCATGCACCCATCGGGCCATTGGCATTGCCAGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCTTGGGCGGATCTGGAACGGGAAGAAAc                                                                                                                             <  1:135595/150‑1 (MQ=255)
                           gTGGGGCAATACGTTGTGATGTACCAACCATGCACCCATCGGGCCATTGGCATTGCCAGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAAcc                                                                                                                            >  1:4505807/1‑150 (MQ=255)
                           gTGGGGCAATACGTTGTGATGTACCAACCATGCACCCATCGGGCCATTGGCATTGCCAGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAAcc                                                                                                                            >  1:3360662/1‑150 (MQ=255)
                            tGGGGCAATACGTTGTGATGTACCAACCATGCACCCATCGGGCCATTGGCATTGCCAGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCa                                                                                                                           >  2:4629872/1‑150 (MQ=255)
                             ggggCAATACGTTGTGATGTACCAACCATGCACCCATCGGGCCATTGGCATTGCCAGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCAt                                                                                                                          >  2:4040496/1‑150 (MQ=255)
                             ggggCAATACGTTGTGATGTACCAACCATGCACCCATCGGGCCATTGGCATTGCCAGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCAt                                                                                                                          <  1:3412063/150‑1 (MQ=255)
                             ggggCAAGACGTTGTGATGTACCAACCATGCACCCATCGGGCCATTGGCATTGCCAGTCACCGGATCTTCCACAATACCACTCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCAt                                                                                                                          <  2:3559062/150‑1 (MQ=255)
                                 cAATTCGTTGTGATGTACCATACTTGCACCCATCTGGCCATTGGCATTGCAAGTCACCGTATATTCCACAATACCAATCGCAGGCGTGATCATGCGTCATTAGGTTTCGTTTTTGCTTGGTCGGTTCTGGTAAGGTTATAAACCATTGct                                                                                                                      <  2:734990/150‑2 (MQ=255)
                                 cAATACGTTGTGATGTACCAACCATGCACCCATCGGGCCATTGGCATTGCCAGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCTTGGGCGGATCTGGAACGGGAAGAAACCATTGCa                                                                                                                      <  2:1151215/150‑1 (MQ=255)
                                   aTACGTTGTGATGTACCAACCATGCACCCATCGGGCCATTGGCATTGCCAGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGc                                                                                                                    <  2:4253635/150‑1 (MQ=255)
                                    tACGTTGTGATGTACCAACCATGCACCCATCGGGCCATTGGCATTGCCAGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGcc                                                                                                                   >  1:1400550/1‑150 (MQ=255)
                                       gTTGTGATGTACCAACCATGCACCCATCGGGCCATTGGCATTGCCAGTCACCGGATCTTCCACACTACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAAt                                                                                                                <  1:945610/150‑1 (MQ=255)
                                        ttGTGATGTACCAACCATGCACCCATCGGGCCATTGGCATTGCCAGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATc                                                                                                               >  1:3144985/1‑150 (MQ=255)
                                          gtgATGTACCAACCATGCACCCATCGGGCCATTGGCATTGCCAGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCtt                                                                                                             >  2:3272236/1‑150 (MQ=255)
                                             aTGTACCAACCATGCACCCATCGGGCCATTGGCATTGCCAGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCttttt                                                                                                          <  1:590506/150‑1 (MQ=255)
                                               gTACCAACCATGCACCCATCGGGCCATTGGCATTGCCAGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGc                                                                                                        <  2:3917703/150‑1 (MQ=255)
                                                tACCAACCATGCACCCATCGGGCCATTGGCATTGCCAGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCt                                                                                                       >  2:3744431/1‑150 (MQ=255)
                                                   caaccaTGCACCCCTCGGGCCATTGGCAGTGCCAGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCTTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGAt                                                                                                    <  1:3751219/150‑1 (MQ=255)
                                                   caaccaTGCACCCATCGGGCCATTGGCATTGCCAGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCTTGGGCGGATCTGGAACGGGAAGAAAACATTGCAGCCAATCTTTTTGCTGAt                                                                                                    >  1:3628936/1‑150 (MQ=255)
                                                      ccaTGCACCCATCGGGCCATTGGCATTGCCAGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACTGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGc                                                                                                 <  1:1434458/150‑1 (MQ=255)
                                                      ccaTGCACCCATCGGGCCATTGGCATTGCCAGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGc                                                                                                 <  1:1312203/150‑1 (MQ=255)
                                                        aTGCACCCATCGGGCCATTGGCATTGCCAGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCgg                                                                                               <  2:4984997/150‑1 (MQ=255)
                                                         tGCACCCATCGGGCCATTGGCATTGCCAGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGt                                                                                              >  1:1632044/1‑150 (MQ=255)
                                                         tGCACCCATCGGGCCATTGGCATTGCCAGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGt                                                                                              <  1:2602968/150‑1 (MQ=255)
                                                          gCACCCATCGGGCCATTGGCATTGCCAGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCTTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTc                                                                                             <  1:2505075/150‑1 (MQ=255)
                                                            aCCCATCGGGCCATTGGCATTGCCAGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCTTGGTCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAg                                                                                           <  1:2091815/150‑1 (MQ=255)
                                                                aTCGGGCCATTGGCATTGCCAGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCa                                                                                       <  2:3478056/150‑1 (MQ=255)
                                                                 tCGGGCCATTGGCATTGCCAGTCACCGGATCTTCCACAATCCCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCAt                                                                                      <  1:386452/150‑1 (MQ=255)
                                                                 tCGGGCCATTGGCATTGCCAGTCACCGGATCGTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCTGCCAATCTTTTTGCTGATAGCGGTCAGCGCAt                                                                                      <  2:2410331/150‑1 (MQ=255)
                                                                  cGGGCCATTGGCATTGCCAGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCTTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCAtt                                                                                     <  1:4358581/150‑1 (MQ=255)
                                                                  cGGGCCATTGGCATTGCCAGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCTTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCAtt                                                                                     <  2:1707177/150‑1 (MQ=255)
                                                                     gCCATTGGCATTGCCAGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCTTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAg                                                                                  <  1:4861040/150‑1 (MQ=255)
                                                                     gCCATTGGCATTGCCAGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAg                                                                                  >  1:2912793/1‑150 (MQ=255)
                                                                      ccATTGGCATTGCCAGTCACCGGATCTTCCACCAGCCCCCTCGCCGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCAGTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAgg                                                                                 <  2:1068101/150‑1 (MQ=255)
                                                                      ccATTGGCATTGCCAGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAgg                                                                                 <  2:3639772/150‑1 (MQ=255)
                                                                       cATTGGCATTGCCAGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCTTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGt                                                                                >  2:4170272/1‑150 (MQ=255)
                                                                          tGGCATTGCCAGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCTTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAg                                                                             <  1:3957451/150‑1 (MQ=255)
                                                                           ggCATTGCCAGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAgg                                                                            <  2:714790/150‑1 (MQ=255)
                                                                              aTTGCCAGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGa                                                                         >  1:708769/1‑150 (MQ=255)
                                                                                 gCCAGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAg                                                                      <  2:3199721/150‑1 (MQ=255)
                                                                                 gCCAGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAg                                                                      <  1:3070178/150‑1 (MQ=255)
                                                                                    aGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGGGc                                                                   <  2:2739221/150‑1 (MQ=255)
                                                                                     gTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGGGCg                                                                  >  1:1347634/1‑150 (MQ=255)
                                                                                       cACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCTTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGGGCGTc                                                                <  1:1213025/150‑1 (MQ=255)
                                                                                       cACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGGGCGTc                                                                <  1:42959/150‑1 (MQ=255)
                                                                                            gATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCTTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGGGCGTCGatat                                                           >  2:4058520/1‑150 (MQ=255)
                                                                                               cTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGGGCGTCGATATCCa                                                        <  1:1506213/150‑1 (MQ=255)
                                                                                                   cacaATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGGGCGTCGATATCCACTTc                                                    >  1:5134049/1‑150 (MQ=255)
                                                                                                    acaATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGGGCGTCGATATCCACTTcc                                                   <  1:1773430/150‑1 (MQ=255)
                                                                                                    acaATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGGGCGTCGATATCCACTTcc                                                   <  2:3831267/150‑1 (MQ=255)
                                                                                                    acaATACCAATCGCAGGCGAGAACATGCGACCATCGGGTTCGTTGTTGCGTGGGCGGATCTGGAACGGGAAGAAACCAGTGCAGCCCATCTTTTTGCTGATAGCGGTCCGCGCAGGAAGGTCAGGCGAACGGGCGTCGAGATCCCCTTcc                                                   >  2:440901/1‑150 (MQ=255)
                                                                                                     caATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCTTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGGGCGTCGATATCCACTTCCg                                                  >  1:2200959/1‑150 (MQ=255)
                                                                                                     caATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGGGCGTCGATATCCACTTCCg                                                  <  1:104981/150‑1 (MQ=255)
                                                                                                      aaTACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGGGCGTCGATATCCACTTCCgg                                                 >  2:2890868/1‑150 (MQ=255)
                                                                                                      aaTACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGGGCGTCGATATCCACTTCCgg                                                 >  1:3183908/1‑150 (MQ=255)
                                                                                                        tACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGGGCGTCGATATCCACTTCCGGtt                                               <  2:1350018/150‑1 (MQ=255)
                                                                                                        tACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGGGCGTCGATATCCACTTCCGGtt                                               <  2:1454568/150‑1 (MQ=255)
                                                                                                         aCCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGGGCGTCGATATCCACTTCCGGttt                                              >  2:2590106/1‑150 (MQ=255)
                                                                                                           cAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGGGCGTCGATATCCACTTCCGGTTTCa                                            >  1:3529526/1‑150 (MQ=255)
                                                                                                              tCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGGGCGTCGATATCCACTTCCGGTTTCAGTg                                         <  1:2452793/150‑1 (MQ=255)
                                                                                                                   ggCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGGGCGTCGATATCCACTTCCGGTTTCAGTGGGatc                                    <  1:4540537/150‑1 (MQ=255)
                                                                                                                   ggCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGGGCGTCGATATCCACTTCCGGTTTCAGTGGGatc                                    <  1:2298492/150‑1 (MQ=255)
                                                                                                                   ggCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGGGCGTCGATATCCACTTCCGGTTTCAGTGGGatc                                    <  2:4827868/150‑1 (MQ=255)
                                                                                                                   ggCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGGGCGTCGATATCCACTTCCGGTTTCAGTGGGatc                                    <  2:3968564/150‑1 (MQ=255)
                                                                                                                      gagaACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGGGCGTCGATATCCACTTCCGGTTTCAGTGGGatcatc                                 >  2:652026/1‑150 (MQ=255)
                                                                                                                      gagaACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGGGCGTCGATATCCACTTCCGGTTTCAGTGGGatcatc                                 <  2:607956/150‑1 (MQ=255)
                                                                                                                         aaCATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGGGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACt                              <  2:547757/150‑1 (MQ=255)
                                                                                                                          aCATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGGGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACtt                             >  1:1957876/1‑150 (MQ=255)
                                                                                                                            aTGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGGGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACtttt                           >  1:1810675/1‑150 (MQ=255)
                                                                                                                            aTGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGGGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACtttt                           >  2:2716683/1‑150 (MQ=255)
                                                                                                                                gACCATCGGTTTCGTTTTTGCTTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGGGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAgt                       >  2:693301/1‑150 (MQ=255)
                                                                                                                                 aCCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGGGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAgtg                      <  2:2868242/150‑1 (MQ=255)
                                                                                                                                  ccATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGGGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAgtgt                     <  2:3379965/150‑1 (MQ=255)
                                                                                                                                      cGGTTTCGTTTTTGCTTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGGGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAGTGTCCtg                 >  1:4077875/1‑150 (MQ=255)
                                                                                                                                        gTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGGGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAGTGTCCtgtt               >  2:942025/1‑150 (MQ=255)
                                                                                                                                        gTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGGGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAGTGTCCtgtt               >  2:194087/1‑150 (MQ=255)
                                                                                                                                          ttCGTTTTTGCTTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGGGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAGTGTCCtgttgt             >  2:1531985/1‑150 (MQ=255)
                                                                                                                                          ttCGTTTTTGCTTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGGGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAGTGTCCtgttgt             >  1:101775/1‑150 (MQ=255)
                                                                                                                                          ttCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGGGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAGTGTCCtgttgt             >  2:4943011/1‑150 (MQ=255)
                                                                                                                                                tttGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGGGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAGTGTCCTGTTGTTGCCAc       >  1:2032271/1‑150 (MQ=255)
                                                                                                                                                 ttGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGGGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAGTGTCCTGTTGTTGCCAcc      <  2:1587071/150‑1 (MQ=255)
                                                                                                                                                   gCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGGGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAGTGTCCTGTTGTTGCCACCTg    <  1:2660644/150‑1 (MQ=255)
                                                                                                                                                     cTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGGGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAGTGTCCTGTTGTTGCCACCTGaa  <  2:4865637/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
GCCTTTAACACGCAACACGTTGCCATCGTGGGGCAATACGTTGTGATGTACCAACCATGCACCCATCGGGCCATTGGCATTGCCAGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGGGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAGTGTCCTGTTGTTGCCACCTGAA  >  CP009273/1529357‑1529655

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑