BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	1,610,766	C→T	7.7%	S249S (AGC→AGT)	yneK →	uncharacterized protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	1,610,766	0	C	T	7.7%	917.2 / 48.2	259	S249S (AGC→AGT)	yneK	uncharacterized protein
Reads supporting (aligned to +/- strand): ref base C (124/115); new base T (6/14); total (130/129)
Fisher's exact test for biased strand distribution p-value = 6.65e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.90e-01

Only 100 of 264 total aligned reads displayed.

AATTTTATCTCTCGCGGTAATTTTGTCATACTCCCCCGATTATGGAATAACTCTGAGGTTCACAGATGGTTCGTCAATAAAGATCCTAACTTGATCACCGCCATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGCCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATCCCTTTTAGCAGAACAAGAATCTCCTCATTTTCAGAACTTGTTTTTATTTTTCGCCAATATGTTATTGCACTATCACCAATTTATGAA  >  CP009273/1610617‑1610915

aaTTTTATCTCTCGCGGTAATTTTGTCATACTCCCCCGATTATGGAATAACTCTGAGGTTCACAGATGGTTCGTCAATAAAGATCCTAACTTGATCACCGCCATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGc                                                                                                                                                       <  1:1107811/150‑1 (MQ=255)

aaTTTTATCTCTCGCGGTAATTTTGTCATACTCCCCCGATTATGGAATAACTCTGAGGTTCACAGATGGTTCGTCAATAAAGATCCTAACTTGATCACCGCCATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGc                                                                                                                                                       >  1:4258979/1‑150 (MQ=255)

   tttATCTCTCGCGGTAATTTTGTCATACTCCCCCGATTATGGAATAACTCTGAGGTTCACAGATGGTTCGTCAATAAAGATCCTAACTTGATCACCGCCATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGCCTg                                                                                                                                                    >  2:2548104/1‑150 (MQ=255)

    ttATCTCTCGCGGTAATTTTGTCATACTCCCCCGATTATGGAATAACTCTGAGGTTCACAGATGGTTCGTCAATAAAGATCCTAACTTGATCACCGCCATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGCCTGa                                                                                                                                                   >  1:576256/1‑150 (MQ=255)

      aTCTCTCGCGGTAATTTTGTCATACTCCCCCGATTATTGAATATCTCTGAGGTTCACAGATGGTTCGTCAATAAAGATCCTAACTTGATCACCGCCATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGCCTGATg                                                                                                                                                 <  1:4199394/150‑1 (MQ=255)

      aTCTCTCGCGGTAATTTTGTCATACTCCCCCGATTATGGAAGAACTCTGAGGTTCACAGATGGTTCGTCAATAAAGATCCTAACTTGATCACCGCCATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGTCTGATg                                                                                                                                                 <  1:4902305/150‑1 (MQ=255)

       tctctcGCGGTAATTTTGTCATACTCCCCCGATTATGGAATAACTCTGAGGTTCACAGATGGTTCGTCAATAAAGATCCTAACTTGATCACCGCCATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGCCTGATgg                                                                                                                                                >  1:4226254/1‑150 (MQ=255)

           tcGCGGTAATTTTGTCATACTCCCCCGATTATGGAATAACTCTGAGGTTCACAGATGGTTCGTCAATAAAGATCCTAACTTGATCACCGCCATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGCCTGATGGATTc                                                                                                                                            <  2:937613/150‑1 (MQ=255)

           tcGCGGTAATTTTGTCATACTCCCCCGATTATGGAATAACTCTGAGGTTCACAGATGGTTCGTCAATAAAGATCCTAACTTGATCACCGCCATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGCCTGATGGATTc                                                                                                                                            <  1:1033742/150‑1 (MQ=255)

           tcGCGGTAATTTTGTCATACTCCCCCGATTATGGAATAACTCTGAGGTTCACAGATGGTTCGTCAATAAAGATCCTAACTTGATCACCGCCATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGCCTGATGGATTc                                                                                                                                            <  1:3603818/150‑1 (MQ=255)

              cgGTAATTTTGTCATACTCCCCCGATTATGGAATAACTCTGAGGTTCACAGGTGGTTCGTCAATAAAGATCCTAACTTGATCACCGCCATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGCCTGATGGATTCAtt                                                                                                                                         >  2:5038006/1‑150 (MQ=255)

              cgGTAATTTTGTCATACTCCCCCGATTATGGAATAACTCTGAGGTTCACAGATGGTTCGTCAATAAAGATCCTAACTTGATCACCGCCATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAATGTCTGATGGATTCAtt                                                                                                                                         >  1:3647322/1‑150 (MQ=255)

              cgGTAATTTTGTCATACTCCCCCGATTATGGAATAACTCTGAGGTTCACAGATGGTTCGTCAATAAAGATCCTAACTTGATCACCGCCATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGCCTGATGGATTCAtt                                                                                                                                         <  1:2895764/150‑1 (MQ=255)

                   aTTTTGTCATACTCCCCCGATTATGGAATAACTCTGAGGTTCACAGATGGTTCGTCAATAAAGATCCTAACTTGATCACCGCCATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGCCTGATGGATTCATTAGGtt                                                                                                                                    <  2:284137/150‑1 (MQ=255)

                      ttGTCATACTCCCCCGATTATGGAATAACTCTGAGGTTCACAGATGGTTCGTCAATAAAGATCCTAACTTGATCACCGCCATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGCCTGATGGATTCATTAGGTTCTa                                                                                                                                 <  1:884936/150‑1 (MQ=255)

                      ttGTCATACTCCCCCGATTATGGAATAACTCTGAGGTTCACAGATGGTTCGTCAATAAAGATCCTAACTTGATCACCGCCATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGCCTGATGGATTCATTAGGTTCTa                                                                                                                                 <  1:252094/150‑1 (MQ=255)

                               tCCCCCGATTATGGAATAACTCTGAGGTTCACAGATGGTTCGTCAATAAAGATCCTAACTTGATCACCGCCATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGTCTGATGGATTCATTAGGTTCTAACAAACATg                                                                                                                        >  2:2208762/1‑150 (MQ=255)

                                cccccGATTATGGAATAACTCTGAGGTTCACAGATGGTTCGTCAATAAAGATCCTAACTTGATCACCGCCATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGCCTGATGGATTCATTAGGTTCTAACAAACATGt                                                                                                                       >  1:4959367/1‑150 (MQ=255)

                                 ccccGATTATGGAATAACTCTGAGGTTCACAGATGGTTCGTCAATAAAGATCCTAACTTGATCACCGCCATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGCCTGATGGATTCATTAGGTTCTAACAAACATGta                                                                                                                      <  1:1983021/150‑1 (MQ=255)

                                 acccGATTATGGAATAACTCTGAGGTTCACAGATGGTTCGTCAATAAAGATCCTAACTTGATCACCGCCATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGCCTGATGGATTCATTTGGTTCTAACAAACATGta                                                                                                                      <  2:737549/149‑1 (MQ=255)

                                   ccGATTATGGAATAACTCTGAGGTTCACAGATGGTTCGTCAATAAAGATCCTAACTTGATCACCGCCATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGCCTGATGGATTCATTAGGTTCTAACAAACATGtact                                                                                                                    <  2:3632566/150‑1 (MQ=255)

                                     gATTATGGAATAACTCTGAGGTTCACAGATGGTTCGTCAATAAAGATCCTAACTTGATCACCGCCATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGCCTGATGGATTCATTAGGTTCTAACAAACATGtactac                                                                                                                  <  1:4390345/150‑1 (MQ=255)

                                      aTTATGGAATAACTCTGAGGTTCACAGATGGTTCGTCAATAAAGATCCTAACTTGATCACCGCCATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGCCTGATGGATTCATTAGGTTCTAACAAACATGTACTAcc                                                                                                                 >  1:398416/1‑150 (MQ=255)

                                       ttATGGAATAACTCTGAGGTTCACAGATGGTTCGTCAATAAAGATCCTAACTTGATCACCGCCATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGCCTGATGGATTCATTAGGTTCTAACAAACATGTACTAccc                                                                                                                <  2:166804/150‑1 (MQ=255)

                                        tATGGAATAACTCTGAGGTTCACAGATGGTTCGTCAATAAATATCCTAACTTGATAACCGCCATTCTCGACATAACGGACAGAGAATTAAAAGAGGATTTGTTGCAAAGCCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCg                                                                                                               <  2:1005709/150‑1 (MQ=255)

                                        tATGGAATAACTCTGAGGTTCACAGATGGTTCGTCAATAAAGATCCTAACTTGATCACCGCCATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGCCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCg                                                                                                               >  1:4521024/1‑150 (MQ=255)

                                            gAATAACTCTGAGGTTCACAGATGGTTCGTCAATAAAGATCCTAACTTGATCACCGCCATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGCCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGt                                                                                                           <  1:3626110/150‑1 (MQ=255)

                                            gAATAACTCTGAGGTTCACAGATGGTTCGTCAATAAAGATCCTAACTTGATCACCGCCATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGCCTGATGGATTCAGTAGGTTCTAACAAACATGTACTCCCCGTAGt                                                                                                           >  2:4151093/1‑150 (MQ=255)

                                              aTAACTCTGAGGTTCACAGATGGTTCGTCAATAAAGATCCTAACTTGATCACCGCCATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGCCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCt                                                                                                         <  1:1856359/150‑1 (MQ=255)

                                                    ctGAGGTTCACAGATGGTTCGTCAATAAAGATCCTAACTTGATCACCGCCATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGCCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCt                                                                                                   >  1:727143/1‑150 (MQ=255)

                                                     tGAGGTTCACAGATGGTTCGTCAATAAAGATCCTAACTTGATCACCGCCATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGCCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTg                                                                                                  >  2:4787063/1‑150 (MQ=255)

                                                      gAGGTTCACAGATGGTTCGTCAATAAAGATCCTAACTTGATCACCGCCATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGCCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGc                                                                                                 >  1:3693735/1‑150 (MQ=255)

                                                       aGGTTCACAGATGGTTCGTCAATAAAGATCCTAACTTGATCACCGCCATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGCCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCt                                                                                                <  2:1713980/150‑1 (MQ=255)

                                                          ttCACAGATGGTTCGTCAATAAAGATCCTAACTTGATCACCGCCATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGCCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCtttt                                                                                             >  1:3867702/1‑150 (MQ=255)

                                                           tCACAGATGGTTCGTCAATAAAGATCCTAACTTGATCACCGCCATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGCCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCttttt                                                                                            >  1:4030389/1‑150 (MQ=255)

                                                              caGATGGTTCGTCAATAAAGATCCTAACTTGATCACCGCCATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGCCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATc                                                                                         <  1:2123639/150‑1 (MQ=255)

                                                              caGATGGTTCGTCAATAAAGATCCTAACTTGATCACCGCCATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGCCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATc                                                                                         >  2:3746909/1‑150 (MQ=255)

                                                                 aTGGTTCGTCAATAAAGATCCTAACTTGATCACCGCCATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGCCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATCCCt                                                                                      <  1:1625935/150‑1 (MQ=255)

                                                                   ggTTCGTCAATAAAGATCCTAACTTGATCACCGCCATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGCCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATCCCttt                                                                                    >  1:1307964/1‑150 (MQ=255)

                                                                    gTTCGTCAATAAAGATCCTAACTTGATCACCGCCATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGCCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATCCCtttt                                                                                   <  1:1228384/150‑1 (MQ=255)

                                                                    gTTCGTCAATAAAGATCCTAACTTGATCACCGCCATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGCCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATCCCtttt                                                                                   <  1:2391499/150‑1 (MQ=255)

                                                                    gTTCGTCAATAAAGATCCTAACTTGATCACCGCCATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGCCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATCCCtttt                                                                                   <  1:3799984/150‑1 (MQ=255)

                                                                     ttCGTCAATAAAGATCCTAACTTGATCACCGCCATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGCCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATCCCTTTTa                                                                                  >  1:1144204/1‑150 (MQ=255)

                                                                      tCGTCAATAAAGATCCTAACTTGATCACCGCCATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGCCTGATGGATTCATTAGGTTCTAACAAACATTTACTACCCGAAGTCTGCATCTGCTTTTTATCCCTTTTAg                                                                                 >  2:3413867/1‑150 (MQ=255)

                                                                       cGTCAATAAAGATCCTAACTTGATCACCGCCATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGCCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATCCCTTTTAGc                                                                                >  1:2842625/1‑150 (MQ=255)

                                                                               aaGATCCTAACTTGATCACCGCCATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGCCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATCCCTTTTAGCAGAACAAg                                                                        >  1:1914643/1‑150 (MQ=255)

                                                                                    ccTAACTTGATCACCGCCATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGCCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATCCCTTTTAGCAGAACAAGAAtct                                                                   >  2:3082247/1‑150 (MQ=255)

                                                                                           tGATCACCGCCATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGCCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATCCCTTTTAGCAGAACAAGAATCTCCTCAtt                                                            <  2:4648002/150‑1 (MQ=255)

                                                                                            gATCACCGCCATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGCCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATCCCTTTTAGCAGAACAAGAATCTCCTCAttt                                                           >  1:4926759/1‑150 (MQ=255)

                                                                                               cACCGCCATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGTCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATCCCTTTTAGCAGAACAAGAATCTCCTCATTTTCa                                                        <  2:2349392/150‑1 (MQ=255)

                                                                                               cACCGCCATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGCCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATCCCTTTTAGCAGAACAAGAATCTCCTCATTTTCa                                                        >  1:4491885/1‑150 (MQ=255)

                                                                                                  cGCCATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGCCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATCCCTTTTAGCAGAACAAGAATCTCCTCATTTTCAGaa                                                     <  2:4385474/150‑1 (MQ=255)

                                                                                                  cGCCATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGCCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATCCCTTTTAGCAGAACAAGAATCTCCTCATTTTCAGaa                                                     <  2:576256/150‑1 (MQ=255)

                                                                                                  cGCCATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGCCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATCCCTTTTAGCAGAACAAGAATCTCCTCATTTTCAGaa                                                     <  2:1070876/150‑1 (MQ=255)

                                                                                                    ccATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGCCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATCCCTTTTAGCAGAACAAGAATCTCCTCATTTTCAGAACt                                                   <  1:4085211/150‑1 (MQ=255)

                                                                                                      aTTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGCCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATCCCTTTTAGCAGAACAAGAATCTCCTCATTTTCAGAACTTg                                                 >  1:2197619/1‑150 (MQ=255)

                                                                                                      aTTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGCCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATCCCTTTTAGCAGAACAAGAATCTCCTCATTTTCAGAACTTg                                                 <  1:1565235/150‑1 (MQ=255)

                                                                                                         ctcGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGCCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATCCCTTTTAGCAGAACAAGAATCTCCTCATTTTCAGAACTTGttt                                              <  1:4787109/150‑1 (MQ=255)

                                                                                                           cGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGCCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATCCCTTTTAGCAGAACAAGAATCTCCTCATTTTCAGAACTTGttttt                                            <  2:1411758/150‑1 (MQ=255)

                                                                                                           cGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGCCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATCCCTTTTAGCAGAACAAGAATCTCCTCATTTTCAGAACTTGttttt                                            <  2:3996506/150‑1 (MQ=255)

                                                                                                           cGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGCCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATCCCTTTTAGCAGAACAAGAATCTCCTCATTTTCAGAACTTGttttt                                            >  2:3910834/1‑150 (MQ=255)

                                                                                                           cGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGCCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATCCCTTTTAGCAGAACAAGAATCTCCTCATTTTCAGAACTTGttttt                                            <  1:4773671/150‑1 (MQ=255)

                                                                                                           cGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGCCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATCCCTTTTAGCAGAACAAGAATCTCCTCATTTTCAGAACTTGttttt                                            >  2:2599288/1‑150 (MQ=255)

                                                                                                            gACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGCCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATCCCTTTTAGCAGAACAAGAATCTCCTCATTTTCAGAACTTGTTTTTa                                           <  1:5038006/150‑1 (MQ=255)

                                                                                                             aCATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGCCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATCCCTTTTAGCAGAACAAGAATCTCCTCATTTTCAGAACTTGTTTTTAt                                          >  2:83553/1‑150 (MQ=255)

                                                                                                             aCATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGCCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATCCCTTTTAGCAGAACAAGAATCTCCTCATTTTCAGAACTTGTTTTTAt                                          >  2:3223448/1‑150 (MQ=255)

                                                                                                               aTAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGCCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATCCCTTTTAGCAGAACAAGAATCTCCTCATTTTCAGAACTTGTTTTTAttt                                        <  2:1324629/150‑1 (MQ=255)

                                                                                                               aTAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGCCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATCCCTTTTAGCAGAACAAGAATCTCCTCATTTTCAGAACTTGTTTTTAttt                                        <  2:4989783/150‑1 (MQ=255)

                                                                                                                 aaCGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGCCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATCCCTTTTAGCAGAACAAGAATCTCCTCATTTTCAGAACTTGTTTTTAttttt                                      >  1:4570095/1‑150 (MQ=255)

                                                                                                                   cGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGCCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATCCCTTTTAGCAGAACAAGAATCTCCTCATTTTCAGAACTTGTTTTTATTTTTCg                                    >  2:2753756/1‑150 (MQ=255)

                                                                                                                    ggACAGCGAATTAAAAGAGGATTTGTTGCAAAGCCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATCCCTTTTAGCAGAACAAGAATCTCCTCATTTTCAGAACTTGTTTTTATTTTTCGc                                   >  2:4295802/1‑150 (MQ=255)

                                                                                                                      aCAGCGAATTAAAAGAGGATTTGTTGCAAAGCCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATCCCTTTTAGCAGAACAAGAATCTCCTCATTTTCAGAACTTGTTTTTATTTTTCGCCa                                 >  1:1094007/1‑150 (MQ=255)

                                                                                                                       cAGCTAATTAAAAGAGGATTTGTTGCAAAGCCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATCCCTTTTAGCAGAACAAGAATCTCCTCATTTTCAGAACTTGTTTTTATTTTTCGCCaa                                <  2:1534865/150‑1 (MQ=255)

                                                                                                                       cAGCGAATTAAAAGAGGATTTGTTGCAAAGCCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATCCCTTTTAGCAGAACAAGAATCTCCTCATTTTCAGAACTTGTTTTTATTTTTCGCCaa                                >  2:2963613/1‑150 (MQ=255)

                                                                                                                         gCGAATTAAAAGAGGATTTGTTGCAAAGCCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATCCCTTTTAGCAGAACAAGAATCTCCTCATTTTCAGAACTTGTTTTTATTTTTCGCCAata                              <  2:1862158/150‑1 (MQ=255)

                                                                                                                          cGAATTAAAAGAGGATTTGTTGCAAAGCCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATCCCTTTTAGCAGAACAAGAATCTCCTCATTTTCAGAACTTGTTTTTATTTTTCGCCAatat                             <  2:3048722/150‑1 (MQ=255)

                                                                                                                          cGAATTAAAAGAGGATTTGTTGCAAAGCCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATCCCTTTTAGCAGAACAAGAATCTCCTCATTTTCAGAACTTGTTTTTATTTTTCGCCAatat                             <  2:269327/150‑1 (MQ=255)

                                                                                                                           gAATTAAAAGAGGATTTGTTGCAAAGCCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATCCCTTTTAGCAGAACAAGAATCTCCTCATTTTCAGAACTTGTTTTTATTTTTCGCCAATATg                            >  1:1291011/1‑150 (MQ=255)

                                                                                                                            aaTTAAAAGAGGATTTGTTGCAAAGCCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATCCCTTTTAGCAGAACAAGAATCTCCTCATTTTCAGAACTTGTTTTTATTTTTCGCCAATATGt                           >  2:3592564/1‑150 (MQ=255)

                                                                                                                             aTTAAAAGAGGATTTGTTGCAAAGCCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATCCCTTTTAGCAGAACAAGAATCTCCTCATTTTCAGAACTTGTTTTTATTTTTCGCCAATATGtt                          <  1:5113456/150‑1 (MQ=255)

                                                                                                                                aaaaGAGGATTTGTTGCAAAGTCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATCCCTTTTAGCAGAACAAGAATCTCCTCATTTTCAGAACTTGTTTTTATTTTTCGCCAATATGTTAtt                       <  1:1762473/150‑1 (MQ=255)

                                                                                                                                 aaaGAGGTTTTGTTGCAAAGCCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATCCCTTTTAGCAGAACAAGAATCTCCTCATTTTCAGAACTTGTTTTTATTTTTCGCCAATATGTTATTg                      <  1:302089/150‑1 (MQ=255)

                                                                                                                                 aaaGAGGATTTGTTCCAAAGCCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATCCCTTTTAGCAGAACAAGAATCTCCTCATTTTCAGAACTTGTTTTTATTTTTCGCCAATATGTTATTg                      >  1:342514/1‑150 (MQ=255)

                                                                                                                                  aaGAGGATTTGTTGCAAAGCCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATCCCTTTTAGCAGAACAAGAATCTCCTCATTTTCAGAACTTGTTTTTATTTTTCGCCAATATGTTATTGc                     <  1:983042/150‑1 (MQ=255)

                                                                                                                                   agagGATTTGTTGCAAAGCCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATCCCTTTTAGCAGAACAAGAATCTCCTCATTTTCAGAACTTGTTTTTATTTTTCGCCAATATGTTATTGCa                    <  2:4977179/150‑1 (MQ=255)

                                                                                                                                     agGATTTGTTGCAAAGCCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATCCCTTTTAGCAGAACAAGAATCTCCTCATTTTCAGAACTTGTTTTTATTTTTCGCCAATATGTTATTGCACt                  >  2:4819339/1‑150 (MQ=255)

                                                                                                                                      ggATTTGTTGCAAAGCCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATCCCTTTTAGCAGAACAAGAATCTCCTCATTTTCAGAACTTGTTTTTATTTTTCGCCAATATGTTATTGCACTa                 >  2:3222446/1‑150 (MQ=255)

                                                                                                                                          ttgttgCAAAGCCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATCCCTTTTAGCAGAACAAGAATCTCCTCATTTTCAGAACTTGTTTTTATTTTTCGCCAATATGTTATTGCACTATCAc             <  2:2832666/150‑1 (MQ=255)

                                                                                                                                           tgttgCAAAGCCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATCCCTTTTAGCAGAACAAGAATCTCCTCATTTTCAGAACTTGTTTTTATTTTTCGCCAATATGTTATTGCACTATCAcc            >  1:1463585/1‑150 (MQ=255)

                                                                                                                                            gttgCAAAGCCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATCCCTTTTAGCAGAACAAGAATCTCCTCATTTTCAGAACTTGTTTTTATTTTTCGCCAATATGTTATTGCACTATCACCa           >  2:3319041/1‑150 (MQ=255)

                                                                                                                                              tgCAAAGCCTGATGGATTCATTAGGTTCTAACAAACATTTACTACCAGAATTATGCATCTTCTTTATATCCCTTTTATCAGAACAAGAATCTCCTCATATTCATAACTTGTTTTTATTTTACGCAAATATTTTATTGAACTATcaacaaa         >  1:3456190/1‑149 (MQ=255)

                                                                                                                                              tgCAAAGCCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATCCCTTTTAGCAGAACAAGAATCTCCTCATTTTCAGAACTTGTTTTTATTTTTCGCCAATATGTTATTGCACTATCACCAAt         >  2:2065281/1‑150 (MQ=255)

                                                                                                                                              tgCAAAGCCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATCCCTTTTAGCAGAACAAGAATCTCCTCATTTTCAGAACTTGTTTTTATTTTTCGCCAATATGTTATTGCACTATCACCAAt         >  2:3161386/1‑150 (MQ=255)

                                                                                                                                                 aaaGCCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATCCCTTTTAGCAGAACAAGAATCTCCTCATTTTCAGAACTTGTTTTTATTTTTCGCCAATATGTTATTGCACTATCACCAATTTa      >  2:1165141/1‑150 (MQ=255)

                                                                                                                                                  aaGCCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATCCCTTTTAGCAGAACAAGAATCTCCTCATTTTCAGAACTTGTTTTTATTTTTCGCCAATATGTTATTGCACTATCACCAATTTAt     >  2:4252935/1‑150 (MQ=255)

                                                                                                                                                  aaGCCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATCCCTTTTAGCAGAACAAGAATCTCCTCATTTTCAGAACTTGTTTTTATTTTTCGCCAATATGTTATTGCACTATCACCAATTTAt     >  2:2306850/1‑150 (MQ=255)

                                                                                                                                                  aaGCCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATCCCTTTTAGCAGAACAAGAATCTCCTCATTTTCAGAACTTGTTTTTATTTTTCGCCAATATGTTATTGCACTATCACCAATTTAt     >  2:380731/1‑150 (MQ=255)

                                                                                                                                                  aaGCCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATCCCTTTTAGCAGAACAAGAATCTCCTCATTTTCAGAACTTGTTTTTATTTTTCGCCAATATGTTATTGCACTATCACCAATTTAt     >  2:3602149/1‑150 (MQ=255)

                                                                                                                                                  aaGCCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATCCCTTTTAGCAGAACAAGAATCTCCTCATTTTCAGAACTTGTTTTTATTTTTCGCCAATATGTTATTGCACTATCACCAATTTAt     <  1:2916081/150‑1 (MQ=255)

                                                                                                                                                   aGCCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATCCCTTTTAGCAGAACAAGAATCTCCTCATTTTCAGAACTTGTTTTTATTTTTCGCCAATATGTTATTGCACTATCACCAATTTATg    >  1:3535741/1‑150 (MQ=255)

AATTTTATCTCTCGCGGTAATTTTGTCATACTCCCCCGATTATGGAATAACTCTGAGGTTCACAGATGGTTCGTCAATAAAGATCCTAACTTGATCACCGCCATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGCCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATCCCTTTTAGCAGAACAAGAATCTCCTCATTTTCAGAACTTGTTTTTATTTTTCGCCAATATGTTATTGCACTATCACCAATTTATGAA  >  CP009273/1610617‑1610915

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`