Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,620,961 T→G 87.2% Q226P (CAA→CCA)  dcp ← dipeptidyl carboxypeptidase II

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,620,9610TG87.2% 917.3 / 72.9 271Q226P (CAA→CCA) dcpdipeptidyl carboxypeptidase II
Reads supporting (aligned to +/- strand):  ref base T (22/14);  new base G (117/118);  total (139/132)
Fisher's exact test for biased strand distribution p-value = 2.16e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 280 total aligned reads displayed.

AGTGTTGCCTGTTGTGCACGGATCTCCACCAGACGTTGAATGATAGCGCGGGTATCATTGGCATCATTTTTTTCCGCTCGCGTCCAGCCCGCAATAAACAGTTTTTCACGCGTCGCACGATCGCGCATTTCGGCAAGCGCCGGTTGTTGGGTGGTATTCAGCAGCGGAATCAGCCATTTGTTATCCAGACCTTTCTCGCGAGCCGCCTCTGCCGCCAGCGCAATCTCTTGCTCACTCATTCCTGCCAGCTGCGCGATATCGTTCACAACCAGACCGCCGGATTTATTTGCTGCCAGT  >  CP009273/1620814‑1621110
                                                                                                                                                   |                                                                                                                                                     
agtGTTGCCTGTTGTGCACGGATCTCCACCAGACGTTGAATGATAGCGCGGGTATCATTGGCATCATTTTTTTCCGCTCGCGTCCAGCCCGCAATAAACAGTTTTTCACGCGTCGCACGATCGCGCATTTCGGCAAGCGCCGGTTGTggg                                                                                                                                                     >  1:3022916/1‑150 (MQ=255)
 gtgtTGCCTGTTGTGCACGGATCTCCACCAGACGTTGAATGATAGCGCGGGTATCATTGGCATCATTTTTTTCCGCTCGCGTCCAGCCCGCAATAAACAGTTTTTCACGCGTCGCACGATCGCGCATTTCGGCAAGCGCCGGTTGTgggg                                                                                                                                                    >  2:915192/1‑150 (MQ=255)
 gtgtTGCCTGTTGTGCACGGATCTCCACCAGACGTTGAATGATAGCGCGGGTATCATTGGCATCATTTTTTTCCGCTCGCGTCCAGCCCGCAATAAACAGTTTTTCACGCGTCGCACGATCGCGCATTTCGGCAAGCGCCGGTTGTTggg                                                                                                                                                    >  1:4366197/1‑150 (MQ=255)
   gtTGCCTGTTGTGCACGGATCTCCACCAGACGTTGAATGATAGCGCGGGTATCATTGGCATCATTTTTTTCCGCTCGCGTCCAGCCCGCAATAAACAGTTTTTCACGCGTCGCACGATCGCGCATTTCGGCAAGCGCCGGTTGTGGggtg                                                                                                                                                  >  1:5025704/1‑150 (MQ=255)
     tGCCTGTTGTGCACGGATCTCCACCAGACGTTGAATGATAGCGCGGGTATCATTGGCATCATTTTTTTCCGCTCGCGTCCAGCCCGCAATAAACAGTTTTTCACGCGTCGCACGATCGCGCATTTCGGCAAGCGCCGGTTGTGGggtggt                                                                                                                                                >  2:2220892/1‑150 (MQ=255)
       ccTGTTGTGCACGGATCTCCACCAGACGTTGAATGATAGCGCGGGTATCATTGGCATCATTTTTTTCCGCTCGCGTCCAGCCCGCAATAAACAGTTTTTCACGCGTCGCACGATCGCGCATTTCGGCAAGCGCCGGTTGTGGGGTGGTAt                                                                                                                                              >  2:1350674/1‑150 (MQ=255)
       ccTGTTGTGCACGGATCTCCACCAGACGTTGAATGATAGCGCGGGTATCATTGGCATCATTTTTTTCCGCTCGCGTCCAGCCCGCAATAAACAGTTTTTCACGCGTCGCACGATCGCGCATTTCGGCAAGCGCCGGTTGTGGGGTGGTAt                                                                                                                                              >  2:2931304/1‑150 (MQ=255)
             gtgCACGGATCTCCACCAGACGTTGAATGATAGCGCGGGTATCATTGGCATCATTTTTTTCCGCTCGCGTCCAGCCCGCAATAAACAGTTTTTCACGCGTCGCACGATCGCGCATTTCGGCAAGCGCCGGTTGTGGGGTGGTATTcagca                                                                                                                                        <  2:4556619/150‑1 (MQ=255)
             gtgCACGGATCTCCACCAGACGTTGAATGATAGCGCGGGTATCATTGGCATCATTTTTTTCCGCTCGCGTCCAGCCCGCAATAAACAGTTTTTCACGCGTCGCACGATCGCGCATTTCGGCAAGCGCCGGTTGTGGGGTGGTATTcagca                                                                                                                                        <  2:3855554/150‑1 (MQ=255)
             gtgCACGGATCTCCACCAGACGTTGAATGATAGCGCGGGTATCATTGGCATCATTTTTTTCCGCTCGCGTCCAGCCCGCAATAAACAGTTTTTCACGCGTCGCACGATCGCGCATTTCGGCAAGCGCCGGTTGTGGGGTGGTATTcagca                                                                                                                                        <  2:3380135/150‑1 (MQ=255)
              tgCACGGATCTCCACCAGACGTTGAATGATAGCGCGGGTATCATTGGCATCATTTTTTTCCGCTCGCGTCCAGCCCGCAATAAACAGTTTTTCACGCGTCGCACGATCGCGCATTTCGGCAAGCGCCGGTTGTGGGGTGGTATTcagcag                                                                                                                                       <  2:4415122/150‑1 (MQ=255)
                cACGGATCTCCACCAGACGTTGAATGATAGCGCGGGTATCATTGGCATCATTTTTTTCCGCTCGCGTCCAGCCCGCAATAAACAGTTTTTCACGCGTCGCACGATCGCGCATTTCGGCAAGCGCCGGTTGTGGGGTGGTATTCAGCAGCg                                                                                                                                     >  1:4112958/1‑150 (MQ=255)
                  cGGATCTCCACCAGACGTTGAATGATAGCGCGGGTATCATTGGCATCATTTTTTTCCGCTCGCGTCCAGCCCGCAATAAACAGTTTTTCACGCGTCGCACGATCGCGCATTTCGGCAAGCGCCGGTTGTGGGGTGGTATTCAGCAGCGGa                                                                                                                                   >  1:4859161/1‑150 (MQ=255)
                  cGGATCTCCACCAGACGTTGAATGATAGCGCGGGTATCATTGGCATCATTTTTTTCCGCTCGCGTCCAGCCCGCAATAAACAGTTTTTCACGCGTCGCACGATCGCGCATTTCGGCAAGCGCCGGTTGTGGGGTGGTATTCAGCAGCGGa                                                                                                                                   <  1:679236/150‑1 (MQ=255)
                   ggATCTCCACCAGACGTTGAATGATAGCGCGGGTATCATTGGCATCATTTTTTTCCGCTCGCGTCCAGCCCGCAATAAACAGTTTTTCACGCGTCGCACGATCGCGCATTTCGGCAAGCGCCGGTTGTTGGGTGGTATTCAGCAGCGGaa                                                                                                                                  <  2:2601079/150‑1 (MQ=255)
                    gATCTCCACCAGACGTTGAATGATAGCGCGGGTATCATTGGCATCATTTTTTTCCGCTCGCGTCCAGCCCGCAATAAACAGTTTTTCACGCGTCGCACGATCGCGCATTTCGGCAAGCGCCGGTTGTGGGGTGGTATTCAGCAGCGGAAt                                                                                                                                 <  2:2732427/150‑1 (MQ=255)
                      tctcCACCAGACGTTGAATGATAGCGCGGGTATCATTGGCATCATTTTTTTCCGCTCGCGTCCAGCCCGCAATAAACAGTTTTTCACGCGTCGCACGATCGCGCATTTCGGCAAGCGCCGGTTGTGGGGTGGTATTCAGCAGCGGAATCa                                                                                                                               <  2:4046534/150‑1 (MQ=255)
                        tcCACCAGACGTTGAATGATAGCGCGGGTATCATTGGCATCATTTTTTTCCGCTCGCGTCCATCCCGCAATAAACAGTTTTTCACGCGTCGCACGATCGCGCATTTCGGCAAGCGCCGGTTGTGGGGTGGTATTCAGCAGCGGAATCAGc                                                                                                                             >  1:3390761/1‑150 (MQ=255)
                        tcCACCAGACGTTGAATGATAGCGCGGGTATCATTGGCATCATTTTTTTCCGCTCGCGTCCAGCCCGCAATAAACAGTTTTTCACGCGTCGCACGATCGCGCATTTCGGCAAGCGCCGGTTGTGGGGTGGTATTCAGCAGCGGAATCAGc                                                                                                                             <  1:1233438/150‑1 (MQ=255)
                        tcCACCAGACGTTGAATGATAGCGCGGGTATCATTGGCATCATTTTTTTCCGCTCGCGTCCAGCCCGCAATAAACAGTTTTTCACGCGTCGCACGATCGCGCATTTCGGCAAGCGCCGGTTGTGGGGTGGTATTCAGCAGCGGAATCAGc                                                                                                                             >  1:1506891/1‑150 (MQ=255)
                          caccaGACGTTGAATGATAGCGCGGGTATCATTGGCATCATTTTTTTCCGCTCGCGTCCAGCCCGCAATAAACAGTTTTTCACGCGTCGCACGATCGCGCATTTCGGCAAGCGCCGGTTGTGGGGTGGTATTCAGCAGCGGAATCAGCCa                                                                                                                           >  1:3902348/1‑150 (MQ=255)
                           accaGACGTTGAATGATAGCGCGGGTATCATTGGCATCATTTTTTTCCGCTCGCGTCCAGCCCGCAATAAACAGTTTTTCACGCGTCGCACGATCGCGCATTTCGGCAAGCGCCGGTTGTTGGGTGGTATTCAGCAGCGGAATCAGCCAt                                                                                                                          <  2:1533634/150‑1 (MQ=255)
                            ccaGACGTTGAATGATAGCGCGGGTATCATTGGCATCATTTTTTTCCGCTCGCGTCCAGCCCGCAATAAACAGTTTTTCACGCGTCGCACGATCGCGCATTTCGGCAAGCGCCGGTTGTGGGGTGGTATTCAGCAGCGGAATCAGCCAtt                                                                                                                         <  2:182776/150‑1 (MQ=255)
                                aCGTTGAATGATAGCGCGGGTATCATTGGCATCATTTTTTTCCGCTCGCGTCCAGCCCGCAATAAACAGTTTTTCACGCGTCGCACGATCGCGCATTTCGGCAAGCGCCGGTTGTGGGGTGGTATTCAGCAGCGGAATCAGCCATTTGtt                                                                                                                     <  1:3003489/150‑1 (MQ=255)
                                 cGTTGAATGATAGCGCGGGTATCATTGGCATCATTTTTTTCCGCTCGCGTCCAGCCCGCAATAAACAGTTTTTCACGCGTCGCACGATCGCGCATTTCGGCAAGCGCCGGTTGTGGGGTGGTATTCAGCAGCGGAATCAGCCATTTGTTa                                                                                                                    <  2:4076039/150‑1 (MQ=255)
                                  gTTGAATGATAGCGCGGGTATCATTGGCATCATTTTTTTCCGCTCGCGTCCAGCCCGCAATAAACAGTTTTTCACGCGTCGCACGATCGCGCATTTCGGCAAGCGCCGGTTGTGGGGTGGTATTCAGCAGCGGAATCAGCCATTTGTTAt                                                                                                                   >  1:4432260/1‑150 (MQ=255)
                                     gAATGATAGCGCGGGTATCATTGGCATCATTTTTTTCCGCTCGCGTCCAGCCCGCAATAAACAGTTTTTCACGCGTCGCACGATCGCGCATTTCGGCAAGCGCCGGTTGTGGGGTGGTATTCAGCAGCGGAATCAGCCATTTGTTATCCa                                                                                                                <  1:3592280/150‑1 (MQ=255)
                                     gAATGATAGCGCGGGTATCATTGGCATCATTTTTTTCCGCTCGCGTCCAGCCCGCAATAAACAGTTTTTCACGCGTCGCACGATCGCGCATTTCGGCAAGCGCCGGTTGTGGGGTGGTATTCAGCAGCGGAATCAGCCATTTGTTATCCa                                                                                                                <  1:44971/150‑1 (MQ=255)
                                      aaTGATAGCGCGGGTATCATTGGCATCATTTTTTTCCGCTCGCGTCCAGCCCGCAATAAACAGTTTTTCACGCGTCGCACGATCGCGCATTTCGGCAAGCGCCGGTTGTGGGGTGGTATTCAGCAGCGGAATCAGCCATTTGTTATCCAg                                                                                                               <  1:3696924/150‑1 (MQ=255)
                                         gATAGCGCGGGTATCATTGGCATCATTTTTTTCCGCTCGCGTCCAGCCCGCAATAAACAGTTTTTCACGCGTCGCACGATCGCGCATTTCGGCAAGCGCCGGTTGTGGGGTGGTATTCAGCAGCGGAATCAGCCATTTGTTATCCAGAcc                                                                                                            <  1:2555382/150‑1 (MQ=255)
                                         gATAGCGCGGGTATCATTGGCATCATTTTTTTCCGCTCGCGTCCAGCCCGCAATAAACAGTTTTTCACGCGTCGCACGATCGCGCATTTCGGCAAGCGCCGGTTGTGGGGTGGTATTCAGCAGCGGAATCAGCCATTTGTTATCCAGAcc                                                                                                            <  2:80452/150‑1 (MQ=255)
                                         gATAGCGCGGGTATCATTGGCATCATTTTTTTCCGCTCGCGTCCAGCCCGCAATAAACAGTTTTTCACGCGTCGCACGATCGCGCATTTCGGCAAGCGCCGGTTGTGGGGTGGTATTCAGCAGCGGAATCAGCCATTTGTTATCCAGAcc                                                                                                            >  2:785940/1‑150 (MQ=255)
                                           tAGCGCGGGTATCATTGGCATCATTTTTTTCCGCTCGCGTCCAGCCCGCAATAAACAGTTTTTCACGCGTCGCACGATCGCGCATTTCGGCAAGCGCCGGTTGTGGGGTGGTATTCAGCAGCGGAATCAGCCATTTGTTATCCAGACCtt                                                                                                          <  2:3759543/150‑1 (MQ=255)
                                            aGCGCGGGTATCATTGGCATCATTTTTTTCCGCTCGCGTCCAGCCCGCAATAAACAGTTTTTCACGCGTCGCACGATCGCGCATTTCGGCAAGCGCCGGTTGTGGGGTGGTATTCAGCAGCGGAATCAGCCATTTGTTATCCAGACCttt                                                                                                         <  1:4056743/150‑1 (MQ=255)
                                            aGCGCGGGTATCATTGGCATCATTTTTTTCCGCTCGCGTCCAGCCCGCAATAAACAGTTTTTCACGCGTCGCACGATCGCGCATTTCGGCAAGCGCCGGTTGTGGGGTGGTATTCAGCAGCGGAATCAGCCATTTGTTATCCAGACCttt                                                                                                         <  1:4055571/150‑1 (MQ=255)
                                             gcgcGGGTATCATTGGCATCATTTTTTTCCGCTCGCGTCCAGCCCGCAATAAACAGTTTTTCACGCGTCGCACGATCGCGCATTTCGGCAAGCGCCGGTTGTGGGGTGGTATTCAGCAGCGGAATCAGCCATTTGTTATCCAGACCTTtc                                                                                                        <  2:2999691/150‑1 (MQ=255)
                                              cgcgGGTATCATTGGCATCATTTTTTTCCGCTCGCGTCCAGCCCGCAATAAACAGTTTTTCACGCGTCGCACGATCGCGCATTTCGGCAAGCGCCGGTTGTGGGGTGGTATTCAGCAGCGGAATCAGCCATTTGTTATCCAGACCTTtct                                                                                                       >  2:1457152/1‑150 (MQ=255)
                                                cgGGTATCATTGGCATCATTTTTTTCCGCTCGCGTCCAGCCCGCAATAAACAGTTTTTCACGCGTCGCACGATCGCGCATTTCGGCAAGCGCCGGTTGTGGGGTGGTATTCAGCAGCGGAATCAGCCATTTGTTATCCAGACCTTTCTcg                                                                                                     >  1:4834726/1‑150 (MQ=255)
                                                   gTATCATTGGCATCATTTTTTTCCGCTCGCGTCCAGCCCGCAATAAACAGTTTTTCACGCGTCGCACGATCGCGCATTTCGGCAAGCGCCGGTTGTGGGGTGGTATTCAGCAGCGGAATCAGCCATTTGTTATCCAGACCTTTCTCGCGa                                                                                                  <  2:1506891/150‑1 (MQ=255)
                                                       cATTGGCATCATTTTTTTCCGCTCGCGTCCAGCCCGCAATAAACAGTTTTTCACGCGTCGCACGATCGCGCATTTCGGCAAGCGCCGGTTGTTGGGTGGTATTCAGCAGCGGAATCAGCCATTTGTTATCCAGACCTTTCTCGCGAgccg                                                                                              <  2:3083965/150‑1 (MQ=255)
                                                       cATTGGCATCATTTTTTTCCGCTCGCGTCCAGCCCGCAATAAACAGTTTTTCACGCGTCGCACGATCGCGCATTTCGGCAAGCGCCGGTTGTGGGGTGGTATTCAGCAGCGGAATCAGCCATTTGTTATCCAGACCTTTCTCGCGAgccg                                                                                              >  1:2489241/1‑150 (MQ=255)
                                                          tGGCATCATTTTTTTCCGCTCGCGTCCAGCCCGCAATAAACAGTTTTTCACGCGTCGCACGATCGCGCATTTCGGCAAGCGCCGGTTGTGGGGTGGTATTCAGCAGCGGAATCAGCCATTTGTTATCCAGACCTTTCTCGCGAGCCGCct                                                                                           >  1:228094/1‑150 (MQ=255)
                                                          tGGCATCATTTTTTTCCGCTCGCGTCCAGCCCGCAATAAACAGTTTTTCACGCGTCGCACGATCGCGCATTTCGGCAAGCGCCGGTTGTGGGGTGGTATTCAGCAGCGGAATCAGCCATTTGTTATCCAGACCTTTCTCGCGAGCCGCct                                                                                           >  1:3275394/1‑150 (MQ=255)
                                                           ggCATCATTTTTTTCCGCTCGCGTCCAGCCCGCAATAAACAGTTTTTCACGCGTCGCACGATCGCGCATTTCGGCAAGCGCCGGTTGTGGGGTGGTATTCAGCAGCGGAATCAGCCATTTGTTATCCAGACCTTTCTCGCGAGCCGCctc                                                                                          <  2:4864368/150‑1 (MQ=255)
                                                               tcatTTTTTTCCGCTCGCGTCCAGCCCGCAATAAACAGTTTTTCACGCGTCGCACGATCGCGCATTTCGGCAAGCGCCGGTTGTGGGGTGGTATTCAGCAGCGGAATCAGCCATTTGTTATCCAGACCTTTCTCGCGAGCCGCCTCTgcc                                                                                      >  2:654778/1‑150 (MQ=255)
                                                                   ttttttCCGCTCGCGTCCAGCCCGCAATAAACAGTTTTTCACGCGTCGCACGATCGCGCATTTCGGCAAGCGCCGGTTGTGGGGTGGTATTCAGCAGCGGAATCAGCCATTTGTTATCCAGACCTTTCTCGCGAGCCGCCTCTGCCGCCa                                                                                  <  1:4861977/150‑1 (MQ=255)
                                                                   ttttttCCGCTCGCGTCCAGCCCGCAATAAACAGTTTTTCACGCGTCGCACGATCGCGCATTTCGGCAAGCGCCGGTTGTGGGGTGGTATTCAGCAGCGGAATCAGCCATTTGTTATCCAGACCTTTCTCGCGAGCCGCCTCTGCCGCCa                                                                                  <  1:1570946/150‑1 (MQ=255)
                                                                    tttttCCGCTCGCGTCCAGCCCGCAATAAACAGTTTTTCACGCGTCGCACGATCGCGCATTTCGGCAAGCGCCGGTTGTGGGGTGGTATTCAGCAGCGGAATCAGCCATTTGTTATCCAGACCTTTCTCGCGAGCCGCCTCTGCCGCCAg                                                                                 <  2:952360/150‑1 (MQ=255)
                                                                     ttttCCGCTCGCGTCCAGCCCGCAATAAACAGTTTTTCACGCGTCGCACGATCGCGCATTTCGGCAAGCGCCGGTTGTGGGGTGGTATTCAGCAGCGGAATCAGCCATTTGTTATCCAGACCTTTCTCGCGAGCCGCCTCTGCCGCCAgc                                                                                <  1:3772104/150‑1 (MQ=255)
                                                                      tttCCGCTCGCGTCCAGCCCGCAATAAACAGTTTTTCACGCGTCGCACGATCGCGCATTTCGGCAAGCGCCGGTTGTGGGGGGGTATTCAGCAGCGGAATCAGCCATTTGTTATCCAGACCTTTCTCGCGAGCCGCCTCTGCCGCCAgcg                                                                               >  2:2764416/1‑150 (MQ=255)
                                                                        tCCGCTCGCGTCCAGCCCGCAATAAACAGTTTTTCACGCGTCGCACGATCGCGCATTTCGGCAAGCGCCGGTTGTGGGGTGGTATTCAGCAGCGGAATCAGCCATTTGTTATCCAGACCTTTCTCGCGAGCCGCCTCTGCCGCCAGCGCa                                                                             >  1:5058012/1‑150 (MQ=255)
                                                                          cGCTCGCGTCCAGCCCGCAATAAACAGTTTTTCACGCGTCGCACGATCGCGCATTTCGGCAAGCGCCGGTTGTGGGGTGGTATTCAGCAGCGGAATCAGCCATTTGTTATCCAGACCTTTCTCGCGAGCCGCCTCTGCCGCCAGCGCAAt                                                                           >  2:3155868/1‑150 (MQ=255)
                                                                            cTCGCGTCCAGCCCGCAATAAACAGTTTTTCACGCGTCGCACGATCGCGCATTTCGGCAAGCGCCGGTTGTGGGGTGGTATTCAGCAGCGGAATCAGCCATTTGTTATCCAGACCTTTCTCGCGAGCCGCCTCTGCCGCCAGCGCAAtct                                                                         >  1:3217074/1‑150 (MQ=255)
                                                                            cTCGCGTCCAGCCCGCAATAAACAGTTTTTCACGCGTCGCACGATCGCGCATTTCGGCAAGCGCCGGTTGTGGGGTGGTATTCAGCAGCGGAATCAGCCATTTGTTATCCAGACCTTTCTCGCGAGCCGCCTCTGCCGCCAGCGCAAtct                                                                         >  2:2347925/1‑150 (MQ=255)
                                                                               gcgTCCAGCCCGCAATAAACAGTTTTTCACGCGTCGCACGATCGCGCATTTCGGCAAGCGCCGGTTGTGGGGTGGTATTCAGCAGCGGAATCAGCCATTTGTTATCCAGACCTTTCTCGCGAGCCGCCTCTGCCGCCAGCGCAATCTCtt                                                                      >  2:2268792/1‑150 (MQ=255)
                                                                                cgTCCAGCCCGCAATAAACAGTTTTTCACGCGTCGCACGATCGCGCATTTCGGCAAGCGCCGGTTGTGGGGTGGTATTCAGCAGCGGAATCAGCCATTTGTTATCCAGACCTTTCTCGCGAGCCGCCTCTGCCGCCAGCGCAATCTCTTg                                                                     >  1:4630412/1‑150 (MQ=255)
                                                                                cgTCCAGCCCGCAATAAACAGTTTTTCACGCGTCGCACGATCGCGCATTTCGGCAAGCGCCGGTTGTGGGGTGGTATTCAGCAGCGGAATCAGCCATTTGTTATCCAGACCTTTCTCGCGAGCCGCCTCTGCCGCCAGCGCAATCTCTTg                                                                     >  1:2386748/1‑150 (MQ=255)
                                                                                   ccAGCCCGCAATAAACAGTTTTTCACGCGTCGCACGATCGCGCATTTCGGCAAGCGCCGGTTGTGGGGTGGTATTCAGCAGCGGAATCAGCCATTTGTTATCCAGACCTTTCTCGCGAGCCGCCTCTGCCGCCAGCGCAATCTCTTGctc                                                                  >  2:3196085/1‑150 (MQ=255)
                                                                                    cAGCCCGCAATAAACAGTTTTTCACGCGTCGCACGATCGCGCATTTCGGCAAGCGCCGGTTGTGGGGTGGTATTCAGCAGCGGAATCAGCCATTTGTTATCCAGACCTTTCTCGCGAGCCGCCTCTGCCGCCAGCGCAATCTCTTGctca                                                                 <  2:1549215/150‑1 (MQ=255)
                                                                                     aGCCCGCAATAAACAGTTTTTCACGCGTCGCACGATCGCGCATTTCGGCAAGCGCCGGTTGTTGGGTGGTATTCAGCAGCGGAATCAGCCATTTGTTATCCAGACCTTTCTCGCGAGCCGCCTCTGCCGCCAGCGCAATCTCTTGctcac                                                                >  2:4945456/1‑150 (MQ=255)
                                                                                     aGCCCGCAATAAACAGTTTTTCACGCGTCGCACGATCGCGCATTTCGGCAAGCGCCGGTTGTGGGGTGGTATTCAGCAGCGGAATCAGCCATTTGTTATCCAGACCTTTCTCGCGAGCCGCCTCTGCCGCCAGCGCAATCTCTTGctcac                                                                >  1:525312/1‑150 (MQ=255)
                                                                                     aGCCCGCAATAAACAGTTTTTCACGCGTCGCACGATCGCGCATTTCGGCAAGCGCCGGTTGTGGGGTGGTATTCAGCAGCGGAATCAGCCATTTGTTATCCAGACCTTTCTCGCGAGCCGCCTCTGCCGCCAGCGCAATCTCTTGctcac                                                                >  1:1164001/1‑150 (MQ=255)
                                                                                       cccGCAATAAACAGTTTTTCACGCGTCGCACGATCGCGCATTTCGGCAAGCGCCGGTTGTGGGGTGGTATTCAGCAGCGGAATCAGCCATTTGTTATCCAGACCTATCTCGCGAGCCGCCTCTGCCGCCAGCGCAATCTCTTGctcactc                                                              >  1:1627777/1‑150 (MQ=255)
                                                                                         cGCAATAAACAGTTTTTCACGCGTCGCACGATCGCGCATTTCGGCAAGCGCCGGTTGTGGGGTGGTATTCAGCAGCGGAATCAGCCATTTGTTATCCAGACCTTTCTCGCGAGCCGCCTCTGCCGCCAGCGCAATCTCTTGCTCACTCAt                                                            <  2:4826482/150‑1 (MQ=255)
                                                                                         cGCAATAAACAGTTTTTCACGCGTCGCACGATCGCGCATTTCGGCAAGCGCCGGTTGTGGGGTGGTATTCAGCAGCGGAATCAGCCATTTGTTATCCAGACCTTTCTCGCGAGCCGCCTCTGCCGCCAGCGCAATCTCTTGCTCACTCAt                                                            <  1:3089452/150‑1 (MQ=255)
                                                                                            aaTAAACAGTTTTTCACGCGTCGCACGATCGCGCATTTCGGCAAGCGCCGGTTGTGGGGTGGTATTCAGCAGCGGAATCAGCCATTTGTTATCCAGACCTTTCTCGCGAGCCGCCTCTGCCGCCAGCGCAATCTCTTGCTCACTCATTcc                                                         <  1:1952575/150‑1 (MQ=255)
                                                                                             aTAAACAGTTTTTCACGCGTCGCACGATCGCGCATTTCGGCAAGCGCCGGTTGTTGGGTGGTATTCAGCAGCGGAATCAGCCATTTGTTATCCAGACCTTTCTCGCGAGCCGCCTCTGCCGCCAGCGCAATCTCTTGCTCACTCATTCCt                                                        <  2:2478189/150‑1 (MQ=255)
                                                                                              tAAACAGTTTTTCACGCGTCGCACGATCGCGCATTTCGGCAAGCGCCGGTTGTGGGGTGGTATTCAGCAGCGGAATCAGCCATTTGTTATCCAGACCTTTCTCGCGAGCCGCCTCTGCCGCCAGCGCAATCTCTTGCTCACTCATTCCTg                                                       >  1:2108804/1‑150 (MQ=255)
                                                                                                aaCAGTTTTTCACGCGTCGCACGATCGCGCATTTCGGCAAGCGCCGGTTGTGGGGTGGTATTCAGCAGCGGAATCAGCCATTTGTTATCCAGACCTTTCTCGCGAGCCGCCTCTGCCGCCAGCGCAATCTCTTGCTCACTCATTCCTGcc                                                     <  1:1618254/150‑1 (MQ=255)
                                                                                                aaCAGTTTGTCACGCGTCGCACGATCGCGCATTTCGGCAAGCGCCGGTTGTGGGGTGGTATTCAGCAGCGGAATCAGCCATTTGTTATCCAGACCTTTCTCGCGAGCCGCCTCTGCCGCCAGCGCAATCTCTTGCTCACTCATTCCTGcc                                                     <  2:1964360/150‑1 (MQ=255)
                                                                                                 aCAGTTTTTCACGCGTCGCACGATCGCGCATTTCGGCAAGCGCCGGTTGTGGGGTGGTATTCAGCAGCGGAATCAGCCATTTGTTATCCAGACCTTTCTCGCGAGCCGCCTCTGCCGCCAGCGCAATCTCTTGCTCACTCATTCCTGCCa                                                    <  2:1675623/150‑1 (MQ=255)
                                                                                                   aGTTTTTCACGCGTCGCACGATCGCGCATTTCGGCAAGCGCCGGTTGTGGGGTGGTATTCAGCAGCGGAATCAGCCATTTGTTATCCAGACCTTTCTCGCGAGCCGCCTCTGCCGCCAGCGCAATCTCTTGCTCACTCATTCCTGCCAGc                                                  >  2:4259920/1‑150 (MQ=255)
                                                                                                     tttttCACGCGTCGCACGATCGCGCATTTCGGCAAGCGCCGGTTGTGGGGTGGTATTCAGCAGCGGAATCAGCCATTTGTTATCCAGACCTTTCTCGCGAGCCGCCTCTGCCGCCAGCGCAATCTCTTGCTCACTCATTCCTGCCAGCTg                                                <  2:1195997/150‑1 (MQ=255)
                                                                                                                  gCACGATCGCGCATTTCGGCAAGCGCCGGTTGTTGGGTGGTATTCAGCAGCGGAATCAGCCATTTGTTATCCAGACCTTTCTCGCGAGCCGCCTCTGCCGCCAGCGCAATCTCTTGCTCACTCATTCCTGCCAGCTGCGCGATATCGTTc                                   >  2:1001685/1‑150 (MQ=255)
                                                                                                                  gCACGATCGCGCATTTCGGCAAGCGCCGGTTGTGGGGTGGTATTCAGCAGCGGAATCAGCCATTTGTTATCCAGACCTTTCTCGCGAGCCGCCTCTGCCGCCAGCGCAATCTCTTGCTCACTCATTCCTGCCAGCTGCGCGATATCGTTc                                   >  1:4844641/1‑150 (MQ=255)
                                                                                                                   cACGATCGCGCATTTCGGCAAGCGCCGGTTGTTGGGTGGTATTCAGCAGCGGAATCAGCCATTTGTTATCCAGACCTTTCTCGCGAGCCGCCTCTGCCGCCAGCGCAATCTCTTGCTCACTCATTCCTGCCAGCTGCGCGATATCGTTca                                  >  2:535163/1‑150 (MQ=255)
                                                                                                                     cGATCGCGCATTTCGGCAAGCGCCGGTTGTGGGGTGGTATTCAGCAGCGGAATCAGCCATTTGTTATCCAGACCTTTCTCGCGAGCCGCCTCTGCCGCCAGCGCAATCTCTTGCTCACTCATTCCTGCCAGCTGCGCGATATCGTTcaca                                >  2:2725543/1‑150 (MQ=255)
                                                                                                                     cGATCGCGCATTTCGGCAAGCGCCGGTTGTGGGGTGGTATTCAGCAGCGGAATCAGCCATTTGTTATCCAGACCTTTCTCGCGAGCCGCCTCTGCCGCCAGCGCAATCTCTTGCTCACTCATTCCTGCCAGCTGCGCGATATCGTTcaca                                <  2:1862574/150‑1 (MQ=255)
                                                                                                                      gATCGCGCATTTCGGCAAGCGCCGGTTGTGGGGTGGTATTCAGCAGCGGAATCAGCCATTTGTTATCCAGACCTTTCTCGCGAGCCGCCTCTGCCGCCAGCGCAATCTCTTGCTCACTCATTCCTGCCAGCTGCGCGATATCGTTCACaa                               >  1:2901301/1‑150 (MQ=255)
                                                                                                                      gATCGCGCATTTCGGCAAGCGCCGGTTGTGGGGTGGTATTCAGCAGCGGAATCAGCCATTTGTTATCCAGACCTTTCTCGCGAGCCGCCTCTGCCGCCAGCGCAATCTCTTGCTCACTCATTCCTGCCAGCTGCGCGATATCGTTCACaa                               >  1:587412/1‑150 (MQ=255)
                                                                                                                      gATCGCGCATTTCGGCAAGCGCCGGTTGTGGGGTGGTATTCAGCAGCGGAATCAGCCAGTTGTTATCCAGCCCTTACTCGCGAGCCGCCTCTGCCGCCAGCGCACTCTCTTGCACACTCATTCCTGCCAGCTCCGCGAGATCGGTCACaa                               >  2:1259267/1‑150 (MQ=255)
                                                                                                                        tCGCGCATTTCGGCAAGCGCCGGTTGTTGGGTGGTATTCAGCAGCGGAATCAGCCATTTGTTATCCAGACCTTTCTCGCGAGCCGCCTCTGCCGCCAGCGCAATCTCTTGCTCACTCATTCCTGCCAGCTGCGCGATATCGTTCACAAcc                             <  2:4738490/150‑1 (MQ=255)
                                                                                                                             cATTTCGGCAAGCGCCGGTTGTGGGGTGGTATTCAGCAGCGGAATCAGCCATTTGTTATCCAGACCTTTCTCGCGAGCCGCCTCTGCCGCCAGCGCAATCTCTTGCTCACTCATTCCTGCCAGCTGCGCGATATCGTTCACAACCAGAcc                        >  1:3379317/1‑150 (MQ=255)
                                                                                                                              aTTTCGGCAAGCGCCGGTTGTGGGGTGGTATTCAGCAGCGGAATCAGCCATTTGTTATCCAGACCTTTCTCGCGAGCCGCCTCTGCCGCCAGCGCAATCTCTTGCTCACTCATTCCTGCCAGCTGCGCGATATCGTTCACAACCAGAccg                       <  2:3455385/150‑1 (MQ=255)
                                                                                                                              aTTTCGGCAAGCGCCGGTTGTGGGGTGGTATTCAGCAGCGGAATCAGCCATTTGTTATCCAGACCTTTCTCGCGAGCCGCCTCTGCCGCCAGCGCAATCTCTTGCTCACTCATTCCTGCCAGCTGCGCGATATCGTTCACAACCAGAccg                       >  1:1655227/1‑150 (MQ=255)
                                                                                                                                ttCGGCAAGCGCCGGTTGTGGGGTGGTATTCAGCAGCGGAATCAGCCATTTGTTATCCAGACCTTTCTCGCGAGCCGCCTCTGCCGCCAGCGCAATCTCTTGCTCACTCATTCCTGCCAGCTGCGCGATATCGTTCACAACCAGAccgcc                     <  2:746413/150‑1 (MQ=255)
                                                                                                                                ttCGGCAAGCGCCGGTTGTGGGGTGGTATTCAGCAGCGGAATCAGCCATTTGTTATCCAGACCTTTCTCGCGAGCCGCCTCTGCCGCCAGCGCAATCTCTTGCTCACTCATTCCTGCCAGCTGCGCGATATCGTTCACAACCAGAccgcc                     <  1:3510070/150‑1 (MQ=255)
                                                                                                                                  cGGCAAGCGCCGGTTGTGGGGTGGTATTCAGCAGCGGAATCAGCCATTTGTTATCCAGACCTTTCTCGCGAGCCGCCTCTGCCGCCAGCGCAATCTCTTGCTCACTCATTCCTGCCAGCTGCTCGATATCGTTCACAACCAGACCGCCgg                   <  2:4732915/150‑1 (MQ=255)
                                                                                                                                  cGGCAAGCGCCGGTTGTGGGGTGGTATTCAGCAGCGGAATCAGCCATTTGTTATCCAGACCTTTCTCGCGAGCCGCCTCTGCCGCCAGCGCAATCTCTTGCTCACTCATTCCTGCCAGCTGCGCGATATCGTTCACAACCAGACCGCCgg                   <  2:3517750/150‑1 (MQ=255)
                                                                                                                                   ggCAAGCGCCGGTTGTGGGGTGGTATTCAGCAGCGGAATCAGCCATTTGTTATCCAGACCTTTCTCGCGAGCCGCCTCTGCCGCCAGCGCAATCTCTTGCTCACTCATTCCTGCCAGCTGCGCGATATCGTTCACAACCAGACCGCCGGa                  <  2:4632699/150‑1 (MQ=255)
                                                                                                                                    gCAAGCGCCGGTTGTGGGGTGGTATTCAGCAGCGGAATCAGCCATTTGTTATCCAGACCTTTCTCGCGAGCCGCCTCTGCCGCCAGCGCAATCTCTTGCTCACTCATTCCTGCCAGCTGCGCGATATCGTTCACAACCAGACCGCCGGat                 >  2:2021184/1‑150 (MQ=255)
                                                                                                                                          gcCGGTTGTGGGGTGGTATTCAGCAGCGGAATCAGCCATTTGTTATCCAGACCTTTCTCGCGAGCCGCCTCTGCCGCCAGCGCAATCTCTTGCTCACTCATTCCTGCCAGCTGCGCGATATCGTTCACAACCAGACCGCCGGatttattt           <  1:965113/150‑1 (MQ=255)
                                                                                                                                           ccGGTTGTTGGGTGGTATTCAGCAGCGGAATCAGCCATTTGTTATCCAGACCTTTCTCGCGAGCCGCCTCTGCCGCCAGCGCAATCTCTTGCTCACTCATTCCTGCCAGCTGCGCGATATCGTTCACAACCAGACCGCCGGATTTATTtg          <  2:727387/150‑1 (MQ=255)
                                                                                                                                           ccGGTTGTGGGGTGGTATTCAGCAGCGGAATCAGCCATTTGTTATCCAGACCTTTCTCGCGAGCCGCCTCTGCCGCCAGCGCAATCTCTTGCTCACTCATTCCTGCCAGCTGCGCGATATCGTTCACAACCAGACCGCCGGATTTATTtg          >  2:358793/1‑150 (MQ=255)
                                                                                                                                           ccGGTTGTGGGGTGGTATTCAGCAGCGGAATCAGCCATTTGTTATCCAGACCTTTCTCGCGAGCCGCCTCTGCCGCCAGCGCAATCTCTTGCTCACTCATTCCTGCCAGCTGCGCGATATCGTTCACAACCAGACCGCCGGATTTATTtg          <  2:5058012/150‑1 (MQ=255)
                                                                                                                                           ccGGTTGTGGGGTGGTATTCAGCAGCGGAATCAGCCATTTGTTATCCAGACCTTTCTCGCGAGCCGCCTCTGCCGCCAGCGCAATCTCTTGCTCACTCATTCCTGCCAGCTGCGCGATATCGTTCACAACCAGACCGCCGGATTTATTtg          >  1:3803039/1‑150 (MQ=255)
                                                                                                                                             ggTTGTTGGGTGGTATTCAGCAGCGGAATCAGCCATTTGTTATCCAGACCTTTCTCGCGAGCCGCCTCTGCCGCCAGCGCAATCTCTTGCTCACTCATTCCTGCCAGCTGCGCGATATCGTTCACAACCAGACCGCCGGATTTATTtgct        >  1:4962744/1‑150 (MQ=255)
                                                                                                                                             ggTTGTGGGGTGGTATTCAGCAGCGGAATCAGCCATTTGTTATCCAGACCTTTCTCGCGAGCCGCCTCTGCCGCCAGCGCAATCTCTTGCTCACTCATTCCTGCCAGCTGCGCGATATCGTTCACAACCAGACCGCCGGATTTATTtgct        >  2:504871/1‑150 (MQ=255)
                                                                                                                                               ttgtggGGTGGTATTCAGCAGCGGAATCAGCCATTTGTTATCCAGACCTTTCTCGCGAGCCGCCTCTGCCGCCAGCGCAATCTCTTGCTCACTCATTCCTGCCAGCTGCGCGATATCGTTCACAACCAGACCGCCGGATTTATTtgctgc      >  2:2127657/1‑150 (MQ=255)
                                                                                                                                                   tgGGTGGTATTCAGCAGCGGAATCAGCCATTTGTTATCCAGACCTTTCTCGCGAGCCGCCTCTGCCGCCAGCGCAATCTCTTGCTCACTCATTCCTGCCAGCTGCGCGATATCGTTCACAACCAGACCGCCGGATTTATTTGCTGCCAGt  <  1:2193692/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                     
AGTGTTGCCTGTTGTGCACGGATCTCCACCAGACGTTGAATGATAGCGCGGGTATCATTGGCATCATTTTTTTCCGCTCGCGTCCAGCCCGCAATAAACAGTTTTTCACGCGTCGCACGATCGCGCATTTCGGCAAGCGCCGGTTGTTGGGTGGTATTCAGCAGCGGAATCAGCCATTTGTTATCCAGACCTTTCTCGCGAGCCGCCTCTGCCGCCAGCGCAATCTCTTGCTCACTCATTCCTGCCAGCTGCGCGATATCGTTCACAACCAGACCGCCGGATTTATTTGCTGCCAGT  >  CP009273/1620814‑1621110

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑