Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,689,180 T→G 34.2% E383D (GAA→GAC)  uidA ← beta‑D‑glucuronidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,689,1800TG34.2% 189.4 / 300.8 247E383D (GAA→GAC) uidAbeta‑D‑glucuronidase
Reads supporting (aligned to +/- strand):  ref base T (76/87);  new base G (38/46);  total (114/133)
Fisher's exact test for biased strand distribution p-value = 8.93e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.47e-01

Only 100 of 248 total aligned reads displayed.

GCGTTGCTTCCGCCAGTGGCGCGAAATATTCCCGTGCACCTTGCGGACGGGTATCCGGTTCGTTGGCAATACTCCACATCACCACGCTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTTTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCGAAACCAATGCCTAAAGAGAGGTTAAAGCCGACAGCAGCAGTTTCATCAATCACCACGATGCCATGTTCATCTGCCCAGTCGAGCATCTCTTC  >  CP009273/1689032‑1689326
                                                                                                                                                    |                                                                                                                                                  
  gTTGCTTCCGCCAGTGGCGCGAAATATTCCCGTGCACCTTGCGGACGGGTATCCGGTTCGTTGGCAATACTCCACATCACCACGCTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTGTcc                                                                                                                                                 >  1:1051671/1‑150 (MQ=255)
   ttGCTTCCGCCAGTGGCGCGAAATATTCCCGTGCACCTTGCGGACGGGTATCCGGTTCGTTGGCAATACTCCACATCACCACGCTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTGTccc                                                                                                                                                <  2:4145445/150‑1 (MQ=255)
    tGCTTCCGCCAGTGGCGCGAAATATTCCCGTGCACCTTGCGGACGGGTATCCGGTTCGTTGGCAATACTCCACATCACCACGCTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTTTcccc                                                                                                                                               <  2:1941773/150‑1 (MQ=255)
    tGCTTCCGCCAGTGGCGCGAAATAGTCCCGTGCACCTTGCGGACGGGTATCCGGTTCGTTGGCAATACTCCACATCACCACGCTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTTTcccc                                                                                                                                               <  1:3613044/150‑1 (MQ=255)
     gCTTCCGCCAGTGGCGCGAAATATTCCCGTGCACCTTGCGGACGGGTATCCGGTTCGTTGGCAATACTCCACATCACCACGCTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTTTCCCCg                                                                                                                                              >  2:1465809/1‑150 (MQ=255)
       ttCCGCCAGTGGCGCGAAATATTCCCGTGCACCTTGCGGACGGGTATCCGGTTCGTTGGCAATACTCCACATCACCACGCTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTGTCCCCGtt                                                                                                                                            >  1:4453907/1‑150 (MQ=255)
       ttCCGCCAGTGGCGCGAAATATTCCCGTGCACCTTGCGGACGGGTATCCGGTTCGTTGGCAATACTCCACATCACCACGCTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTGTCCCCGtt                                                                                                                                            >  1:198053/1‑150 (MQ=255)
           gCCAGTGGCGCGAAATATTCCCGTGCACCTTGCGGACGGGTATCCGGTTCGTTGGCAATACTCCACATCACCACGCTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTTTCCCCGTTGACt                                                                                                                                        <  1:121780/150‑1 (MQ=255)
             cAGTGGCGCGAAATATTCCCGTGCACCTTGCGGACGGGTATCCGGTTCGTTTGCAATACTCCACATCACCACGCTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTGTCCCCGTTGACTGc                                                                                                                                      >  2:4180180/1‑150 (MQ=255)
                    gcgAAATATTCCCGTGCACCTTGCGGACGGGTATCCGGTTCGTTGGCAATACTCCACATCACCACGCTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTTTCCCCGTTGACTGCCTCTTCg                                                                                                                               <  2:3166114/150‑1 (MQ=255)
                      gAAATATTCCCGTGCACCTTGCGGACGGGTATCCGGTTCGTTGGCAATACTCCACATCACCACGCTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTTTCCCCGTTGACTGCCTCTTCGCt                                                                                                                             >  1:4410435/1‑150 (MQ=255)
                      gAAATATTCCCGTGCACCTTGCGGACGGGTATCCGGTTCGTTGGCAATACTCCACATCACCACGCTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTTTCCCCGTTGACTGCCTCTTCGCt                                                                                                                             >  2:2037403/1‑150 (MQ=255)
                       aaaTATTCCCGTGCACCTTGCGGACGGGTATCCGGTTCGTTGGCAATACTCCACATCACCACGCTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTTTCCCCGTTGACTGCCTCTTCGCTg                                                                                                                            >  1:1313644/1‑150 (MQ=255)
                       aaaTATTCCCGTGCACCTTGCGGACGGGTATCCGGTTCGTTGGCAATACTCCACATCACCACGCTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTGTCCCCGTTGACTGCCTCTTCGCTg                                                                                                                            >  2:1983683/1‑150 (MQ=255)
                        aaTATTCCCGTGCACCTTGCGGACGGGTATCCGGTTCGTTGGCAATACTCCACATCACCACGCTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTTTCCCCGTTGACTGCCTCTTCGCTGt                                                                                                                           >  2:2799722/1‑150 (MQ=255)
                        aaTATTCCCGTGCACCTTGCGGACGGGTATCCGGTTCGTTGGCAATACTCCACATCACCACGCTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTTTCCCCGATGACTGCCTCTTCGCTGt                                                                                                                           >  2:2799554/1‑150 (MQ=255)
                         atatTCCCGTGCACCTTGCGGACGGGTATCCGGTTCGTTGGCAATACTCCACATCACCACGCTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTGTCCCCGTTGACTGCCTCTTCGCTGTa                                                                                                                          <  2:4174897/150‑1 (MQ=255)
                          tatTCCCGTGCACCTTGCGGACGGGTATCCGGTTCGTTGGCAATACTCCACATCACCACGCTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTTTCCCCGTTGACTGCCTCTTCGCTGTAc                                                                                                                         >  2:1317054/1‑150 (MQ=255)
                                   gCACCTTGCGGACGGGTATCCGGTTCGTTGGCAATACTCCACATCACCACGCTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTTTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTc                                                                                                                <  2:3405295/150‑1 (MQ=255)
                                      ccTTGCGGACGGGTATCCGGTTCGTTGGCAATACTCCACATCACCACGCTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTTTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGc                                                                                                             <  1:1609649/150‑1 (MQ=255)
                                        ttGCGGACGGGTATCCGGTTCGTTGGCAATACTCCACATCACCACGCTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTTTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCtt                                                                                                           <  1:2521082/150‑1 (MQ=255)
                                         tGCGGACGGGTATCCGGTTCGTTGGCAATACTCCACATCACCACGCTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTGTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCttg                                                                                                          <  1:2940786/150‑1 (MQ=255)
                                         tGCGGACGGGTATCCGGTTCGTTGGCAATACTCCACATCACCACGCGTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTTTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCttg                                                                                                          >  2:3553524/1‑150 (MQ=255)
                                           cGGACGGGTATCCGGTTCGTTGGCAATACTCCACATCACCACGCTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTTTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCttgtt                                                                                                        <  1:3918837/150‑1 (MQ=255)
                                            ggACGGGTATCCGGTTCGTTGGCAATACTCCACATCACCACGCTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTTTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCttgttg                                                                                                       >  1:4109789/1‑150 (MQ=255)
                                              aCGGGTATCCGGTTCGTTGGCAATACTCCACATCACCACGCTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTGTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGcc                                                                                                     <  1:3966125/150‑1 (MQ=255)
                                                gggTATCCGGTTCGTTGGCAATACTCCACATCACCACGCTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTGTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCg                                                                                                   >  2:2597739/1‑150 (MQ=255)
                                                    aTCCGGTTCGTTGGCAATACTCCACATCACCACGCTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTTTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTc                                                                                               <  2:2249474/150‑1 (MQ=255)
                                                      ccGGTTCGTTGGCAATACTCCACATCACCACGCTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTGTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCGa                                                                                             <  1:3225571/150‑1 (MQ=255)
                                                      ccGGTTCGTTGGCAATACTCCACATCACCACGCTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTGTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCGa                                                                                             <  1:4449509/150‑1 (MQ=255)
                                                       cGGTTCGTTGGCAATACTCCACATCACCACGCTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTTTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCGaa                                                                                            >  2:4437140/1‑150 (MQ=255)
                                                        ggTTCGTTGGCAATACTCCACATCACCACGCTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTTTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCGaaa                                                                                           >  1:4664535/1‑150 (MQ=255)
                                                        ggTTCGTTGGCAATACTCCACATCACCACGCTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTTTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCGaaa                                                                                           >  1:1466997/1‑150 (MQ=255)
                                                         gTTCGTTGGCAATACTCCACATCACCACGCTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTTTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCGAAAc                                                                                          <  1:4822521/150‑1 (MQ=255)
                                                          ttCGTTGGCAATACTCCACATCACCACGCTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTTTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCGAAAcc                                                                                         >  2:1296441/1‑150 (MQ=255)
                                                           tCGTTGGCAATACTCCACATCACCACGCTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTTTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCGAAACCa                                                                                        <  1:4303598/150‑1 (MQ=255)
                                                           tCGTTGGCAATACTCCACATCACCACGCTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTTTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCGAAACCa                                                                                        >  1:885261/1‑150 (MQ=255)
                                                                 gCAATACTCCACATCACCACGCTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTTTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCGAAACCAATGCCt                                                                                  <  1:4789983/150‑1 (MQ=255)
                                                                 gCAATACTCCACATCACCACGCTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTTTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCGAAACCAATGCCt                                                                                  >  1:4985574/1‑150 (MQ=255)
                                                                 gCAATACTCCACATCACCACGCTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTGTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCGAAACCAATGCCt                                                                                  <  2:4594168/150‑1 (MQ=255)
                                                                  cAATACTCCACATCACCACGCTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTGTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCGAAACCAATGCCTa                                                                                 <  2:1781051/150‑1 (MQ=255)
                                                                   aaTACTCCACATCACCACGCTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTTTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCGAAACCAATGCCTaa                                                                                >  2:1129078/1‑150 (MQ=255)
                                                                    aTACTCCACATCACCACGCTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTTTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCGAAACCAATGCCTaaa                                                                               >  2:3412996/1‑150 (MQ=255)
                                                                     tACTCCACATCACCACGCTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTTTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCGAAACCAATGCCTAAag                                                                              >  1:3009463/1‑150 (MQ=255)
                                                                      aCTCCACATCACCACGCTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTGTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCGAAACCAATGCCTAAaga                                                                             <  2:2177218/150‑1 (MQ=255)
                                                                      aCTCCACATCACCACGCTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTGTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCGAAACCAATGCCTAAaga                                                                             >  1:1520645/1‑150 (MQ=255)
                                                                        tCCACATCACCACGCTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTTTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCGAAACCAATGCCTAAagaga                                                                           >  1:909477/1‑150 (MQ=255)
                                                                        tCCACATCACCACGCTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTGTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCGAAACCAATGCCTAAagaga                                                                           <  1:1875802/150‑1 (MQ=255)
                                                                          cacaTCACCACGCTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTTTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCGAAACCAATGCCTAAAGAGAgg                                                                         >  1:5117922/1‑150 (MQ=255)
                                                                          cacaTCACCACGCTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTTTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCGAAACCAATGCCTAAAGAGAgg                                                                         <  2:4014004/150‑1 (MQ=255)
                                                                           acaTCACCACGCTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTTTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCGAAACCAATGCCTAAAGAGAGGt                                                                        >  2:4187509/1‑150 (MQ=255)
                                                                             aTCACCACGCTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTGTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCGAAACCAATGCCTAAAGAGAGGTTa                                                                      <  2:255886/150‑1 (MQ=255)
                                                                             aTCACCACGCTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTGTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCGAAACCAATGCCTAAAGAGAGGTTa                                                                      >  1:1706739/1‑150 (MQ=255)
                                                                              tCACCACGCTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTGTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCGAAACCAATGCCTAAAGAGAGGTTaa                                                                     >  1:1761727/1‑150 (MQ=255)
                                                                               caccacGCTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTACGTGCGCTTGCTGAGTGTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCGAAACCAATGCCTAAAGAGAGGTTaaa                                                                    >  2:3773869/1‑150 (MQ=255)
                                                                                  cacGCTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTTTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCGAAACCAATGCCTAAAGAGAGGTTAAAGcc                                                                 <  1:2052231/150‑1 (MQ=255)
                                                                                  cacGCTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTGTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCGAAACCAATGCCTAAAGAGAGGTTAAAGcc                                                                 >  2:1312289/1‑150 (MQ=255)
                                                                                        ggggTGGTTTTGGTCACGCGCGATCAGCTCTTGCCTCGCCTGTACGTGCGCTGGCTGAGTTGCCCCGTGGACGGCCTCGTCGCTGTACAGTTCTTTCGGCTTGTGGCCCGCTTCGAAACCAATGCCTAAAGAGAGGTTAAAGCCGAcagc                                                           <  2:4628741/149‑1 (MQ=255)
                                                                                            tggtTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTTTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCGAAACCAATGCCTAAAGAGAGGTTAAAGCCGAcagcagca                                                       <  2:5040133/150‑1 (MQ=255)
                                                                                            tggtTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTGTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCGAAACCAATGCCTAAAGAGAGGTTAAAGCCGAcagcagca                                                       <  1:1014631/150‑1 (MQ=255)
                                                                                             ggtTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTTTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCGAAACCAATGCCTAAAGAGAGGTTAAAGCCGAcagcagcag                                                      <  2:5021487/150‑1 (MQ=255)
                                                                                              gtTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTGTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCGAAACAAATGCCTAAAGAGAGGTTAAAGCCGACAGCAGCAGt                                                     <  2:1417072/150‑1 (MQ=255)
                                                                                               tttttGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTGTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCGAAACCAATGCCTAAAGAGAGGTTAAAGCCGACAGCAGCAGtt                                                    <  1:4966866/150‑1 (MQ=255)
                                                                                                ttttGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTGTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCGAAACCAATGCCTAAAGAGAGGTTAAAGCCGACAGCAGCAGttt                                                   >  1:314208/1‑150 (MQ=255)
                                                                                                ttttGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTGTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCGAAACCAATGCCTAAAGAGAGGTTAAAGCCGACAGCAGCAGttt                                                   >  1:695757/1‑150 (MQ=255)
                                                                                                ttttGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTGTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTGGTTGCCCGCTTCGAAACCAATGCCTAAAGAGAGGTTAAAGCAGACAGCAGCAGttt                                                   >  1:3267763/1‑150 (MQ=255)
                                                                                                 tttGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTTTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCGAAACCAATGCCTAAAGAGAGGTTAAAGCCGACAGCAGCAGTTtc                                                  <  2:3277403/150‑1 (MQ=255)
                                                                                                           gcTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTTTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCGAAACCAATGCCTAAAGAGAGGTTAAAGCCGACAGCAGCAGTTTCATCAATcacc                                        <  2:3341913/150‑1 (MQ=255)
                                                                                                             tATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTTTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCGAAACCAATGCCTAAAGAGAGGTTAAAGCCGACAGCAGCAGTTTCATCAATcaccac                                      <  2:38571/150‑1 (MQ=255)
                                                                                                             tATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTTTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCGAAACCAATGCCTAAAGAGAGGTTAAAGCCGACAGCAGCAGTTTCATCAATcaccac                                      <  2:825275/150‑1 (MQ=255)
                                                                                                             tATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTGTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCGAAACCAATGCCTAAAGAGAGGTTAAAGCCGACAGCAGCAGTTTCATCAATcaccac                                      >  1:1554354/1‑150 (MQ=255)
                                                                                                              aTCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTGTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCGAAACCAATGCCTAAAGAGAGGTTAAAGCCGACAGCAGCAGTTTCATCAATCACCACg                                     >  2:1652140/1‑150 (MQ=255)
                                                                                                               tCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTGTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCGAAACCAATGCCTAAAGAGAGGTTAAAGCCGACAGCAGCAGTTTCATCAATCACCACGa                                    >  2:2634479/1‑150 (MQ=255)
                                                                                                                 aGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTTTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCGAAACCAATGCCTAAAGAGAGGTTAAAGCCGACAGCAGCAGTTTCATCAATCACCACGATg                                  <  1:2600438/150‑1 (MQ=255)
                                                                                                                 aGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTGTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCGAAACCAATGCCTAAAGAGAGGTTAAAGCCGACAGCAGCAGTTTCATCAATCACCACGATg                                  <  2:3494286/150‑1 (MQ=255)
                                                                                                                  gCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTTTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCGAAACCAATGCCTAAAGAGAGGTTAAAGCCGACAGCAGCAGTTTCATCAATCACCACGATGc                                 >  2:689522/1‑150 (MQ=255)
                                                                                                                   ctctTGAATCGCCTGTACGTGCGCTTGCTGAGTTTCCCCGTTGACTGCCTCTTCGCGGTACAGTTCTTTCGGCTTGGTGCCCGCTTCGAAACCTATGCCTAAAGAGAGGTTAAAGCCGACAGCAGCAGTTTCATCAATCACCACGATGcc                                <  2:2706036/150‑1 (MQ=255)
                                                                                                                    tctTTAATCGCCTGTAAGTGCGCTTGCTGAGTTTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCGAAACCAATGCCTAAAGAGAGGTTAAAGCCGACAGCAGCAGTTTCATCAATCACCACGATGCCa                               <  1:903216/150‑1 (MQ=255)
                                                                                                                     ctTTAATCGCCTGTAAGTGCGCTTGCTGAGTTTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCGAAACCAATGCCTAAAGAGAGGTTAAAGCCGACAGCAGCAGTTTCATCAATCACCACGATGCCAt                              >  1:3246515/1‑150 (MQ=255)
                                                                                                                      tttAATCGCCTGTAAGTGCGCTTGCTGAGTTTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCGAAACCAATGCCTAAAGAGAGGTTAAAGCCGACAGCAGCAGTTTCATCAATCACCACGATGCCATg                             <  1:3510443/150‑1 (MQ=255)
                                                                                                                       ttAATCGCCTGTAAGTGCGCTTGCTGAGTGTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCGAAACCAATGCCTAAAGAGAGGTTAAAGCCGACAGCAGCAGTTTCATCAATCACCACGATGCCATGt                            <  1:1376597/150‑1 (MQ=255)
                                                                                                                          aTCGCCTGTAAGTGCGCTTGCTGAGTGTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCGAAACCAATGCCTAAAGAGAGGTTAAAGCCGACAGCAGCAGTTTCATCAATCACCACGATGCCATGTTCa                         <  2:1024591/150‑1 (MQ=255)
                                                                                                                           tCGCCTGTAAGTGCGCTTGCTGAGTTTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCGAAACCAATGCCTAAAGAGAGGTTAAAGCCGACAGCAGCAGTTTCATCAATCACCACGATGCCATGTTCAt                        <  1:2833266/150‑1 (MQ=255)
                                                                                                                             gCCTGTAAGTGCGCTTGCTGAGTTTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCGAAACCAATGCCTAAAGAGAGGTTAAAGCCGACAGCAGCAGTTTCATCAATCACCACGATGCCATGTTCATCt                      <  1:5044188/150‑1 (MQ=255)
                                                                                                                                 gTAAGTGCGCTTGCTGAGTTTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCGAAACCAATGCCTAAAGAGAGGTTAAAGCCGACAGCAGCAGTTTCATCAATCACCACGATGCCATGTTCATCTGccc                  <  2:1853283/150‑1 (MQ=255)
                                                                                                                                 gTAAGTGCGCTTGCTGAGTTTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCGAAACCAATGCCTAAAGAGAGGTTAAAGCCGACAGCAGCAGTTTCATCAATCACCACGATGCCATGTTCATCTGccc                  <  2:3042192/150‑1 (MQ=255)
                                                                                                                                 gTAAGTGCGCTTGCTGAGTTTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCGAAACCAATGCCTAAAGAGAGGTTAAAGCCGACAGCAGCAGTTTCATCAATCACCACGATGCCATGTTCATCTGccc                  <  2:2104003/150‑1 (MQ=255)
                                                                                                                                  tAAGTGCGCTTGCTGAGTGTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCGAAACCAATGCCTAAAGAGAGGTTAAAGCCGACAGCAGCAGTTTCATCAATCACCACGATGCCATGTTCATCTGCCCa                 <  1:1162102/150‑1 (MQ=255)
                                                                                                                                     gTGCGCTTGCTGAGTTTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCGAAACCAATGCCTAAAGAGAGGTTAAAGCCGACAGCAGCAGTTTCATCAATCACCACGATGCCATGTTCATCTGCCCAGTc              <  2:1131141/150‑1 (MQ=255)
                                                                                                                                     gTGCGCTTGCTGAGTTTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCGAAACCAATGCCTAAAGAGAGGTTAAAGCCGACAGCAGCAGTTTCATCAATCACCACGATGCCATGTTCATCTGCCCAGTc              <  1:3046902/150‑1 (MQ=255)
                                                                                                                                       gcgcTTGCTGAGTTTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCGAAACCAATGCCTAAAGAGAGGTTAAAGCCGACAGCAGCAGTTTCATCAATCACCACGATGCCATGTTCATCTGCCCAGTCGa            <  1:4564475/150‑1 (MQ=255)
                                                                                                                                         gcTTGCTGAGTTTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTGCGGCTTGTTGCCCGCTTCGAAACCAATGCCTAAAGAGAGGTTAAAGCCGACAGCAGCAGTTTCATCAATCACCACGATGCCATGTTCATCTGCCCAGTCGANc          <  1:3553524/150‑1 (MQ=255)
                                                                                                                                         gcTTGCTGAGTGTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCGAAACCAATGCCTAAAGAGAGGTTAAAGCCGACAGCAGCAGTTTCATCAATCACCACGATGCCATGTTCATCTGCCCAGTCGAGc          >  2:4790956/1‑150 (MQ=255)
                                                                                                                                          cTTGCTGAGTTTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCGAAACCAATGCCTAAAGAGAGGTTAAAGCCGACAGCAGCAGTTTCATCAATCACCACGATGCCATGTTCATCTGCCCAGTCGAGCa         <  2:3230212/150‑1 (MQ=255)
                                                                                                                                           ttGCTGAGTTTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCGAAACCAATGCCTAAAGAGAGGTTAAAGCCGACAGCAGCAGTTTCATCAATCACCACGATGCCATGTTCATCTGCCCAGTCGAGCAt        >  1:1058909/1‑150 (MQ=255)
                                                                                                                                            tGCTGAGTTTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCGAAACCAATGCCTAAAGAGAGGTTAAAGCCGACAGCAGCAGTTTCATCAATCACCACGATGCCATGTTCATCTGCCCAGTCGAGCAtc       <  1:4397162/150‑1 (MQ=255)
                                                                                                                                               tGAGTTTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCGAAACCAATGCCTAAAGAGAGGTTAAAGCCGACAGCAGCAGTTTCATCAATCACCACGATGCCATGTTCATCTGCCCAGTCGAGCATctct    <  2:3325124/150‑1 (MQ=255)
                                                                                                                                                gAGTGTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCGAAACCAATGCCTAAAGAGAGGTTAAAGCCGACAGCAGCAGTTTCATCAATCACCACGATGCCATGTTCATCTGCCCAGTCGAGCATCTCtt   <  1:2204921/150‑1 (MQ=255)
                                                                                                                                                 aGTTTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCGAAACCAATGCCTAAAGAGAGGTTAAAGCCGACAGCAGCAGTTTCATCAATCACCACGATGCCATGTTCATCTGCCCAGTCGAGCATCTCTTc  <  2:368281/150‑1 (MQ=255)
                                                                                                                                                 aGTTTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCGAAACCAATGCCTAAAGAGAGGTTAAAGCCGACAGCAGCAGTTTCATCAATCACCACGATGCCATGTTCATCTGCCCAGTCGAGCATCTCTTc  <  2:2889305/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
GCGTTGCTTCCGCCAGTGGCGCGAAATATTCCCGTGCACCTTGCGGACGGGTATCCGGTTCGTTGGCAATACTCCACATCACCACGCTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTTTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCGAAACCAATGCCTAAAGAGAGGTTAAAGCCGACAGCAGCAGTTTCATCAATCACCACGATGCCATGTTCATCTGCCCAGTCGAGCATCTCTTC  >  CP009273/1689032‑1689326

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑