Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,690,981 G→T 19.1% A110E (GCG→GAG)  uidR ← transcriptional repressor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,690,9810GT19.1% 847.2 / 138.0 296A110E (GCG→GAG) uidRtranscriptional repressor
Reads supporting (aligned to +/- strand):  ref base G (141/98);  new base T (35/22);  total (176/120)
Fisher's exact test for biased strand distribution p-value = 7.66e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 300 total aligned reads displayed.

GCAGTAAACGTGAAGTCATTGCCGTGTTGATGTCTGGGCTTATCTCGCCTTTTTGCTGGGCATCACGCATCCGCTGGGCAACAAATTCCGTGATCGTCATATGCTTATTTTTAAGCATGGCGGCGACCTGTGGGTTACGCATCCCTTCCGCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATGGGTGAGAGAGACAATGGACTCGACCATATAGTCAACGAAATGAATCCCTTCAATCGGTTCCCGGAAACGGGCCAGCGCCCTCTCCTGGTCCTGTAAGATAATCGCC  >  CP009273/1690832‑1691130
                                                                                                                                                     |                                                                                                                                                     
   gTAAACGTGAAGTCATTGCCGTGTTGATGTCTGGGCTTATCTCGCCTTTTTGCTGGGCATCACGCATCCGCTGGGCAACAAATTCCGTGATCGTCATATGCTTATTTTTAAGCATGGCGGCGACCTGTGGGTTACGCATCCCTTCCGCCa                                                                                                                                                    >  2:4451899/1‑150 (MQ=255)
    tAAACGTGAAGTCATTGCCGTGTTGATGTCTGGGCTTATCTCGCCTTTTTGCTGGGCATCACGCATCCGCTGGGCAACAAATTCCGTGATCGTCATATGCTTATTTTTAAGCATGGCGGCGACCTGTGGGTTACGCATCCCTTCCGCCAt                                                                                                                                                   >  1:635883/1‑150 (MQ=255)
     aaaCGTGAAGTCATTGCCGTGTTGATGTCTGGGCTTATCTCGCCTTTTTGCTGGGCATCACGCATCCGCTGGGCAACAAATTCCGTGATCGTCATATGCTTATTTTTAAGCATGGCGGCGACCTGTGGGTTACGCATCCCTTCCTCCATa                                                                                                                                                  <  1:3910855/150‑1 (MQ=255)
           gAAGTCATTGCCGTGTTGATGTCTGGGCTTATCTCGCCTTTTTGCTGGGCATCACGCATCCGCTGGGCAACAAATTCCGTGATCGTCATATGCTTATTTTTAAGCATGGCGGCGACCTGTGGGTTACGCATCCCTTCCGCCATAATTTCa                                                                                                                                            >  1:2304163/1‑150 (MQ=255)
            aaGTCATTGCCGTGTTGATGTCTGGGCTTATCTCGCCTTTTTGCTGGGCATCACGCATCCGCTGGGCAACAAATTCCGTGATCGTCATATGCTTATTTTTAAGCATGGCGGCGACCTGTGGGTTACGCATCCCTTCCGCCATAATTTCaa                                                                                                                                           >  2:4720064/1‑150 (MQ=255)
              gTCATTGCCGTGTTGATGTCTGGGCTTATCTCGCCTTTTTGCTGGGCATCACGCATCCGCTGGGCAACAAATTCCGTGATCGTCATATGCTTATTTTTAAGCATGGCGGCGACCTGTGGGTTACGCATCCCTTCCTCCATAATTTCAacc                                                                                                                                         <  2:5046924/150‑1 (MQ=255)
                 aTTGCCGTGTTGATGTCTGGGCTTATCTCGCCTTTTTGCTGGGCATCACGCATCCGCTGGGCAACAAATTCCGTGATCGTCATATGCTTATTTTTAAGCATGGCGGCGACCTGTGGGTTACGCATCCCTTCCTCCATAATTTCAaccacc                                                                                                                                      >  1:304438/1‑150 (MQ=255)
                 aTTGCCGTGTTGATGTCTGGGCTTATCTCGCCTTTTTGCTGGGCATCACGCATCCGCTGGGCAACAAATTCCGTGATCGTCATATGCTTATTTTTAAGCATGGCGGCGACCTGTGGGTTACGCATCCCTTCCGCCATAATTTCAaccacc                                                                                                                                      <  2:231876/150‑1 (MQ=255)
                  ttGCCGTGTTGATGTCTGGGCTTATCTCGCCTTTTTGCTGGTCATCACGCATCCGCTGGGCAACAAATTCCGTGATCGTCATATGCTTATTTTTAAGCATGGCGGCGACCTGTTGGTTACGCATCCCTTCCGCCATAATTTCAAccacca                                                                                                                                     <  1:2351464/150‑1 (MQ=255)
                  ttGCCGTGTTGATGTCTGGGCTTATCTCGCCTTTTTGCTGGGCATCACGCATCCGCTGGGCAACAAATTCCGTGATCGTCATATGCTTATTTTTAAGCATGGCGGCGACCTGTGGGTTACGCATCCCTTCCGCCATAATTTCAAccacca                                                                                                                                     <  1:2456530/150‑1 (MQ=255)
                   tGCCGTGTTGATGTCTGGGCTTATCTCGCCTTTTTGCTGGGCATCACGCATCCGCTGGGCAACAAATTCCGTGATCGTCATATGCTTATTTTTAAGCATGGCGGCGACCTGTGGGTTACGCATCCCTTCCGCCATAATTTCAACCACCAg                                                                                                                                    <  2:3125551/150‑1 (MQ=255)
                           tGATGTCTGGGCTTATCTCGCCTTTTTGCTGGGCATCACGCATCCGCTGGGCAACAAATTCCGTGATCGTCATATGCTTATTTTTAAGCATGGCGGCGACCTGTGGGTTACGCATCCCTTCCTCCATAATTTCAACCACCAGCGCCCGtt                                                                                                                            >  2:4348474/1‑150 (MQ=255)
                           tGATGTCTGGGCTTATCTCGCCTTTTTGCTGGGCATCACGCATCCGCTGGGCAACAAATTCCGTGATCGTCATATGCTTATTTTTAAGCATGGCGGCGACCTGTGGGTTACGCATCCCTTCCGCCATAATTTCAACCACCAGCGCCCGtt                                                                                                                            >  1:4031165/1‑150 (MQ=255)
                           tGATGTCTGGGCTTATCTCGCCTTTTTGCTGGGCATCACGCATCCGCTGGGCAACAAATTCCGTGATCGTCATATGCTTATTTTTAAGCATGGCGGCGACCTGTGGGTTACGCATCCCTTCCGCCATAATTTCAACCACCAGCGCCCGtt                                                                                                                            <  2:1607273/150‑1 (MQ=255)
                              tGTCTGGGCTTATCTCGCCTTTTTGCTGGGCATCACGCATCCGCTGGGCAACAAATTCCGTGATCGTCATATGCTTATTTTTAAGCATGGCGGCGACCTGTGGGTTACGCATCCCTTCCGCCATAATTTCAACCACCAGCGCCCGTTGTc                                                                                                                         <  2:1304211/150‑1 (MQ=255)
                                     gCTTATCTCGCCTTTTTGCTGGGCATCACGCATCCGCTGGGCAACAAATTCCGTGATCGTCATATGCTTATTTTTAAGCATGGCGGCGACCTGTGGGTTACGCATCCCTTCCTCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGc                                                                                                                  >  1:4677678/1‑150 (MQ=255)
                                         aTCTCGCCTTTTTGCTGGGCATCACGCATCCGCTGGGCAACAAATTCCGTGATCGTCATATGCTTATTTTTAAGCATGGCGGCGACCTGTGGGTTACGCATCCCTTCCTCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCa                                                                                                              <  2:541251/150‑1 (MQ=255)
                                         aTCTCGCCTTTTTGCTGGGCATCACGCATCCGCTGGGCAACAAATTCCGTGATCGTCATATGCTTATTTTTAAGCATGGCGGCGACCTGTGGGTTACGCATCCCTTCCTCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCa                                                                                                              >  2:3741737/1‑150 (MQ=255)
                                         aTCTCGCCTTTTTGCTGGGCATCACGCATCCGCTGGGCAACAAATTCCGTGATCGTCATATGCTTATTTTTAAGCATGGCGGCGACCTGTGGGTTACGCATCCCTTCCGCCATAATTTCAACCACCCGCGCCCGTTGTCCAAAGGCTTCa                                                                                                              >  2:4762993/1‑150 (MQ=255)
                                         aTCTCGCCTTTTTGCTGGGCATCACGCATCCGCTGGGCAACAAATTCCGTGATCGTCATATGCTTATTTTTAAGCATGGCGGCGACCTGTGGGTTACGCATCCCTTCCGCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCa                                                                                                              >  2:3218492/1‑150 (MQ=255)
                                           ctcGCCTTTTTGCTGGGCATCACGCATCCGCTGGGCAACAAATTCCGTGATCGTCATATGCTTATTTTTAAGCATGGCGGCGACCTGTGGGTTACGCATCCCTTCCGCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATg                                                                                                            >  2:1937530/1‑150 (MQ=255)
                                           ctcGCCTTTTTGCTGGGCATCACGCATCCGCTGGGCAACAAATTCCGTGATCGTCATATGCTTATTTTTAAGCATGGCGGCGACCTGTGGGTTACGCATCCCTTCCGCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATg                                                                                                            >  1:3932169/1‑150 (MQ=255)
                                           ctcGCCTTTTTGCTGGGCATCACGCATCCGCTGGGCAACAAATTCCGTGATCGTCATATGCTTATTTTTAAGCATGGCGGCGACCTGTGGGTTACGCATCCCTTCCGCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATg                                                                                                            >  1:112804/1‑150 (MQ=255)
                                            tcGCCTTTTTGCTGGGCATCACGCATCCGCTGGGCAACAAATTCCGTGATCGTCATATGCTTATTTTTAAGCATGGCGGCGACCTGTGGGTTACGCATCCCTTCCTCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTGCATgg                                                                                                           >  2:4133911/1‑150 (MQ=255)
                                            tcGCCTTTTTGCTGGGCATCACGCATCCGCTGGGCAACAAATTCCGTGATCGTCATATGCTTATTTTTAAGCATGGCGGCGACCTGTGGGTTACGCATCCCTTCCGCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATgg                                                                                                           <  1:4397076/150‑1 (MQ=255)
                                             cGCCTTTTTGCTGGGCATCACGCATCCGCTGGGCAACAAATTCCGTGATCGTCATATGCTTATTTTTAAGCATGGCGGCGACCTGTGGGTTACGCATCCCTTCCGCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATggg                                                                                                          >  1:4319893/1‑150 (MQ=255)
                                              gCCTTTTTGCTGGGCATCACGCATCCGCTGGGCAACAAATTCCGTGATCGTCATATGCTTATTTTTAAGCATGGCGGCGACCTGTGGGTTACGCATCCCTTCCTCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATGGGt                                                                                                         >  1:297720/1‑150 (MQ=255)
                                               ccTTTTTGCTGGGCATCACGCATCCGCTGGGCAACAAATTCCGTGATCGTCATATGCTTATTTTTAAGCATGGCGGCGACCTGTGGGTTACGCATCCCTTCCGCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATGGGTg                                                                                                        >  2:2769914/1‑150 (MQ=255)
                                               ccTTTTTGCTGGGCATCACGCATCCGCTGGGCAACAAATTCCGTGATCGTCATATGCTTATTTTTAAGCATGGCGGCGACCTGTGGGTTACGCATCCCTTCCGCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATGGGTg                                                                                                        >  2:3296130/1‑150 (MQ=255)
                                               ccTTTTTGCTGGGCATCACGCATCCGCTGGGCAACAAATTCCGTGATCGTCATATGCTTATTTTTAAGCATGGCGGCGACCTGTGGGTTACGCATCCCTTCCGCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATGGGTg                                                                                                        >  1:2528318/1‑150 (MQ=255)
                                                  ttttGCTGGGCATCACGCATCCGCTGGGCAACAAATTCCGTGATCGTCATATGCTTATTTTTAAGCATGGCGGCGACCTGTGGGTTACGCATCCCTTCCGCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATGGGTgaga                                                                                                     <  2:1816295/150‑1 (MQ=255)
                                                   tttGCTGGGCATCACGCATCCGCTGGGCAACAAATTCCGTGATCGTCATATGCTTATTTTTAAGCATGGCGGCGACCTGTGGGTTACGCATCCCTTCCGCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATGGGTgagag                                                                                                    <  2:940427/150‑1 (MQ=255)
                                                      gCTGGGCATCACGCATCCGCTGGGCAACAAATTCCGTGATCGTCATATGCTTATTTTTAAGCATGGCGGCGACCTGTGGGTTACGCATCCCTTCCGCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATGGGTgagagaga                                                                                                 <  1:4561413/150‑1 (MQ=255)
                                                       cTGGGCATCACGCATCCGCTGGGCAACAAATTCCGTGATCGTCATATGCTTATTTTTAAGCATGGCGGCGACCTGTGGGTTACGCATCCCTTCCGCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATGGGTGAGAGAGAc                                                                                                >  2:2817900/1‑150 (MQ=255)
                                                        tGGGCATCACGCATCCGCTGGGCAACAAATTCCGTGATCGTCATATGCTTATTTTTAAGCATGGCGGCGACCTGTGGGTTACGCATCCCTTCCGCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATGGGTGAGAGAGACa                                                                                               >  2:3995620/1‑150 (MQ=255)
                                                          ggCATCACGCATCCGCTGGGCAACAAATTCCGTGATCGTCATATGCTTATTTTTAAGCATGGCGGCGACCTGTGGGTTACGCATCCCTTCCGCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATGGGTGAGAGAGACAAt                                                                                             <  1:2406208/150‑1 (MQ=255)
                                                              tCACGCATCCGCTGGGCAACAAATTCCGTGATCGTCATATGCTTATTTTTAAGCATGGCGGCGACCTGTGGGTTACGCATCCCTTCCGCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATGGGTGAGAGAGACAATGGAc                                                                                         <  2:3368740/150‑1 (MQ=255)
                                                               cACGCATCCGCTGGGCAACAAATTCCGTGATCGTCATATGCTTATTTTTAAGCATGGCGGCGACCTGTGGGTTACGCATCCCTTCCGCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATGGGTGAGAGAGACAATGGACt                                                                                        <  1:1378607/150‑1 (MQ=255)
                                                                aCGCATCCGCTGGGCAACAAATTCCGTGATCGTCATATGCTTATTTTTAAGCATGGCGGCGACCTGTGGGTTACGCATCCCTTCCGCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATGGGTGAGAGAGACAATGGACTc                                                                                       >  2:4060086/1‑150 (MQ=255)
                                                                 cGCATCCGCTGGGCAACAAATTCCGTGATCGTCATATGCTTATTTTTAAGCATGGCGGCGACCTGTGGGTTACGCATCCCTTCCGCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATGGGTGAGAGAGACAATGGACTCg                                                                                      >  1:1865263/1‑150 (MQ=255)
                                                                 cGCATCCGCTGGGCAACAAATTCCGTGATCGTCATATGCTTATTTTTAAGCATGGCGGCGACCTGTGGGTTACGCATCCCTTCCGCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATGGGTGAGAGAGACAATGGACTCg                                                                                      >  1:1865107/1‑150 (MQ=255)
                                                                  gCATCCGCTGGGCAACAAATTCCGTGATCGTCATATGCTTATTTTTAAGCATGGCGGCGACCTGTGGGTTACGCATCCCTTCCGCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATGGGTGAGAGAGACAATGGACTCGa                                                                                     >  2:1001339/1‑150 (MQ=255)
                                                                    aTCCGCTGGGCAACAAATTCCGTGATCGTCATATGCTTATTTTTAAGCATGGCGGCGACCTGTGGGTTACGCATCCCTTCCGCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCTTGGGTGAGAGAGACAATGGACTCGAcc                                                                                   >  2:3869119/1‑150 (MQ=255)
                                                                    aTCCGCTGGGCAACAAATTCCGTGATCGTCATATGCTTATTTTTAAGCATGGCGGCGACCTGTGGGTTACGCATCCCTTCCGCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATGGGTGAGAGAGACAATGGACTCGAcc                                                                                   <  2:832908/150‑1 (MQ=255)
                                                                    aTCCGCTGGGCAACAAATTCCGTGATCGTCATATGCTTATTTTTAAGCATGGCGGCGACCTGTGGGTTACGCATCCCTTCCGCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATGGGTGAGAGAGACAATGGACTCGAcc                                                                                   <  2:2153366/150‑1 (MQ=255)
                                                                     tCCGCTGGGCAACAAATTCCGTGATCGTCATATGCTTATTTTTAAGCATGGCGGCGACCTGTGGGTTACGCATCCCTTCCGCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATGGGTGAGAGAGACACTGGACTCGACCa                                                                                  >  1:1646930/1‑150 (MQ=255)
                                                                      ccGCTGGGCAACAAATTCCGTGATCGTCATATGCTTATTTTTAAGCATGGCGGCGACCTGTGGGTTACGCATCCCTTCCTCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATGGGTGAGAGAGACAATGGACTCGACCat                                                                                 <  1:18129/150‑1 (MQ=255)
                                                                      ccGCTGGGCAACAAATTCCGTGATCGTCATATGCTTATTTTTAAGCATGGCGGCGACCTGTGGGTTACGCATCCCTTCCTCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATGGGTGAGAGAGACAATGGACTCGACCat                                                                                 <  1:831789/150‑1 (MQ=255)
                                                                      ccGCTGGGCAACAAATTCCGTGATCGTCATATGCTTATTTTTAAGCATGGCGGCGACCTGTGGGTTACGCATCCCTTCCGCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATGGGTGAGAGAGACAATGGACTCGACCat                                                                                 <  1:2819323/150‑1 (MQ=255)
                                                                       cGCTGGGCAACAAATTCCGTGATCGTCATATGCTTATTTTTAAGCATGGCGGCGACCTGTGGGTTACGCATCCCTTCCGCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATGGGTGAGAGAGACAATGGACTCGACCata                                                                                >  1:1972344/1‑150 (MQ=255)
                                                                          tGGGCAACAAATTCCGTGATCGTCATATGCTTATTTTTAAGCATGGCGGCGACCTGTGGGTTACGCATCCCTTCCGCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATGGGTGAGAGAGACAATGGACTCGACCATATAg                                                                             >  2:2487403/1‑150 (MQ=255)
                                                                             gCAACAAATTCCGTGATCGTCATATGCTTATTTTTAAGCATGGCGGCGACCTGTGGGTTACGCATCCCTTCCGCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATGGGTGAGAGAGACAATGGACTCGACCATATAGTCa                                                                          >  1:2099544/1‑150 (MQ=255)
                                                                              caacaaATTCCGTGATCGTCATATGCTTATTTTTAAGCATGGCGGCGACCTGTGGGTTACGCATCCCTTCCGCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATGGGTGAGAGAGACAATGGACTCGACCATCTAGTCaa                                                                         >  2:3151536/1‑150 (MQ=255)
                                                                               aacaaATTCCGTGATCGTCATATGCTTATTTTTAAGCATGGCGGCGACCTGTGGGTTACGCATCCCTTCCGCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATGGGTGAGAGAGACAATGGACTCGACCATATAGTCAAc                                                                        >  2:1429119/1‑150 (MQ=255)
                                                                               aacaaATTCCGTGATCGTCATATGCTTATTTTTAAGCATGGCGGCGACCTGTGGGTTACGCATCCCTTCCGCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATGGGTGAGAGAGACAATGGACTCGACCATATAGTCAAc                                                                        <  2:4957693/150‑1 (MQ=255)
                                                                               aacaaATTCCGTGATCGTCATATGCTTATTTTTAAGCATGGCGGCGACCTGTGGGTTACGCATCCCTTCCGCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATGGGTGAGAGAGACAATGGACTCGACCATATAGTCAAc                                                                        >  2:772816/1‑150 (MQ=255)
                                                                                 caaATTCCGTGATCGTCATATGCTTATTTTTAAGCATGGCGGCGACCTGTGGGTTACGCATCCCTTCCGCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATGGGTGAGAGAGACAATGGACTCGACCATATAGTCAACGa                                                                      >  2:2634564/1‑150 (MQ=255)
                                                                                  aaaTTCCGTGATCGTCATATGCTTATTTTTAAGCATGGCGGCGACCTGTGGGTTACGCATCCCTTCCGCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATGGGTGAGAGAGACAATGGACTCGACCATATAGTCAACGaa                                                                     >  1:3545604/1‑150 (MQ=255)
                                                                                   aaTTCCGTGATCGTCATATGCTTATTTTTAAGCATGGCGGCGACCTGTGGGTTACGCATCCCTTCCTCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATGGGTGAGAGAGACAATGGACTCGACCATATAGTCAACGaaa                                                                    <  2:2452211/150‑1 (MQ=255)
                                                                                   aaTTCCGTGATCGTCATATGCTTATTTTTAAGCATGGCGGCGACCTGTGGGTTACGCATCCCTTCCTCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATGGGTGAGAGAGACAATGGACTCGACCATATAGTCAACGaaa                                                                    >  1:130652/1‑150 (MQ=255)
                                                                                   aaTTCCGTGATCGTCATATGCTTATTTTTAAGCATGGCGGCGACCTGTGGGTTACGCATCCCTTCCTCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATGGGTGAGAGAGACAATGGACTCGACCATATAGTCAACGaaa                                                                    >  1:1188856/1‑150 (MQ=255)
                                                                                        cGTGATCGTCATATGCTTATTTTTAAGCATGGCGGCGACCTGTGGGTTACGCATCCCTTCCGCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATGGGTGAGAGAGACAATGGACTCGACCATATAGTCAACGAAATGAAt                                                               >  1:157374/1‑150 (MQ=255)
                                                                                          tGATCGTCATATGCTTATTTTTAAGCATGGCGGCGACCTGTGGGTTACGCATCCCTTCCGCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATGGGTGAGAGAGACAATGGACTCGACCATATAGTCAACGAAATGAATcc                                                             >  2:4602625/1‑150 (MQ=255)
                                                                                           gATCGTCATATGCTTATTTTTAAGCATGGCGGCGACCTGTGGGTTACGCATCCCTTCCTCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATGGGTGAGAGAGACAATGGACTCGACCATATAGTCAACGAAATGAATccc                                                            >  1:4890582/1‑150 (MQ=255)
                                                                                           gATCGTCATATGCTTATTTTTAAGCATGGCGGCGACCTGTGGGTTACGCATCCCTTCCGCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATGGGTGAGAGAGACAATGGACTCGACCATATAGTCAACGAAATGAATccc                                                            >  1:1244780/1‑150 (MQ=255)
                                                                                            aTCGTCATATGCTTATTTTTAAGCATGGCGGCGACCTGTGGGTTACGCATCCCTTCCTCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATGGGTGAGAGAGACAATGGACTCGACCATATAGTCAACGAAATGAATCCCt                                                           >  1:1232779/1‑150 (MQ=255)
                                                                                             tCGTCATATGCTTATTTTTAAGCATGGCGGCGACCTGTGGGTTACGCATCCCTTCCGCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATGGGTGAGAGAGACAATGGACTCGACCATATAGTCAACGAAATGAATCCCtt                                                          >  2:63230/1‑150 (MQ=255)
                                                                                              cGTCATATGCTTATTTTTAAGCATGGCGGCGACCTGTGGGTTACGCATCCCTTCCGCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATGGGTGAGAGAGACAATGGACTCGACCATATAGTCAACGAAATGAATCCCTTc                                                         <  2:2258699/150‑1 (MQ=255)
                                                                                                tCATATGCTTATTTTTAAGCATGGCGGCGACCTGTGGGTTACGCATCCCTTCCGCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATGGGTGAGAGAGACAATGGACTCGACCATATAGTCAACGAAATGAATCCCTTCaa                                                       >  1:1666403/1‑150 (MQ=255)
                                                                                                  atatGCTTATTTTTAAGCATGGCGGCGACCTGTGGGTTACGCATCCCTTCCGCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATGGGTGAGAGAGACAATGGACTCGACCATATAGTCAACGAAATGAATCCCTTCAATc                                                     >  2:118942/1‑150 (MQ=255)
                                                                                                   tatGCTTATTTTTAAGCATGGCGGCGACCTGTGGGTTACGCATCCCTTCCGCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATGGGTGAGAGAGACAATGGACTCGACCATATAGTCAACGAAATGAATCCCTTCAATCg                                                    >  2:3160459/1‑150 (MQ=255)
                                                                                                   tatGCTTATTTTTAAGCATGGCGGCGACCTGTGGGTTACGCATCCCTTCCGCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATGGGTGAGAGAGACAATGGACTCGACCATATAGTCAACGAAATGAATCCCTTCAATCg                                                    >  2:3652490/1‑150 (MQ=255)
                                                                                                    atGCTTATTTTTAAGCATGGCGGCGACCTGTGGGTTACGCATCCCTTCCGCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATGGGTGAGAGAGACAATGGACTCGACCATATAGTCAACGAAATGAATCCCTTCAATCgg                                                   >  2:2433453/1‑150 (MQ=255)
                                                                                                     tGCTTATTTTTAAGCATGGCGGCGACCTGTGGGTTACGCATCCCTTCCTCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATGGGTGAGAGAGACAATGGACTCGACCATATAGTCAACGAAATGAATCCCTTCAATCGGt                                                  >  2:3944550/1‑150 (MQ=255)
                                                                                                     tGCTTATTTTTAAGCATGGCGGCGACCTGTGGGTTACGCATCCCTTCCGCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATGGGTGAGAGAGACAATGGACTCGACCATATAGTCAACGAAATGAATCCCTTCAATCGGt                                                  >  1:3100938/1‑150 (MQ=255)
                                                                                                     tGCTTATTTTTAAGCATGGCGGCGACCTGTGGGTTACGCATCCCTTCCGCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATGGGTGAGAGAGACAATGGACTCGACCATATAGTCAACGAAATGAATCCCTTCAATCGGt                                                  <  1:4775751/150‑1 (MQ=255)
                                                                                                     tGCTTATTTTTAAGCATGGCGGCGACCTGTGGGTTACGCATCCCTTCCGCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATGGGTGAGAGAGACAATGGACTCGACCATATAGTCAACGAAATGAATCCCTTCAATCGGt                                                  >  1:3635065/1‑150 (MQ=255)
                                                                                                        ttATTTTTAAGCATGGCGGCGACCTGTGGGTTACGCATCCCTTCCGCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATGGGTGAGAGAGACAATGGACTCGACCATATAGTCAACGAAATGAATCCCTTCAATCGGTTcc                                               <  2:2685797/150‑1 (MQ=255)
                                                                                                          aTTTTTAAGCATGGCGGCGACCTGTGGGTTACGCATCCCTTCCTCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATGGGTGAGAGAGACAATGGACTCGACCATATAGTCAACGAAATGAATCCCTTCAATCGGTTCCCg                                             >  2:1670776/1‑150 (MQ=255)
                                                                                                              ttAAGCATGGCGGCGACCTGTGGGTTACGAATCCCTTCCGCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATGGGTGAGAGAGACAATGGACTCGACCATATAGTCAACGAAATGAATCCCTTCAATCGGTTCCCGGaaa                                         <  1:2672144/150‑1 (MQ=255)
                                                                                                               tAAGCATGGCGGCGACCTGTGGGTTACGCATCCCTTCCGCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATGGGTGAGAGAGACAATGGACTCGACCATATAGTCAACGAAATGAATCCCTTCAATCGGTTCCCGGAAAc                                        >  2:3506569/1‑150 (MQ=255)
                                                                                                                aaGCATGGCGGCGACCTGTGGGTTACGCATCCCTTCCGCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATGGGTGAGAGAGACAATGGACTCGACCATATAGTCAACGAAATGAATCCCTTCAATCGGTTCCCGGAAACg                                       >  2:4413055/1‑150 (MQ=255)
                                                                                                                aaGCATGGCGGCGACCTGTGGGTTACGCATCCCTTCCGCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATGGGTGAGAGAGACAATGGACTCGACCATATAGTCAACGAAATGAATCCCTTCAATCGGTTCCCGGAAACg                                       >  1:5096214/1‑150 (MQ=255)
                                                                                                                 aGCATGGCGGCGACCTGTGGGTTACGCATCCCTTCCGCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATGGGTGAGAGAGACAATGGACTCGACCATATAGTCAACGAAATGAATCCCTTCAATCGGTTCCCGGAAACgg                                      >  2:304622/1‑150 (MQ=255)
                                                                                                                   cATGGCGGCGACCTGTGGGTTACGCATCCCTTCCGCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATGGGTGAGAGAGACAATGGACTCGACCATATAGTCAACGAAATGAATCCCTTCAATCGGTTCCCGGAAACGGGc                                    >  2:4533710/1‑150 (MQ=255)
                                                                                                                    aTGGCGGCGACCTGTGGGTTACGCATCCCTTCCTCCATAATTTCAACCACCAGCGACCGTTGTCCAAAGGCTTCATGGGTGAGAGAGACAATGGACTCGACCATATAGTCAACGAAATGAATCCCTTCAATCGGTTCCCGGAAACGGGcc                                   >  2:1865250/1‑150 (MQ=255)
                                                                                                                     tGGCGGCGACCTGTGGGTTACGCATCCCTTCCGCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATGGGTGAGAGAGACAATGGACTCGACCATATAGTCAACGAAATGAATCCCTTCAATCGGTTCCCGGAAACGGGCCa                                  >  1:3779130/1‑150 (MQ=255)
                                                                                                                        cggcgACCTGTGGGTTACGCATCCCTTCCGCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATGGGTGAGAGAGACAATGGACTCGACCATATAGTCAACGAAATGAATCCCTTCAATCGGTTCCCGGAAACGGGCCAgcg                               <  2:3232030/150‑1 (MQ=255)
                                                                                                                         ggcgACCTGTGGGTTACGCATCCCTTCCGCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATGGGTGAGAGAGACAATGGACTCGACCATATAGTCAACGAAATGAATCCCTTCAATCGGTTCCCGGAAACGGGCCAgcgc                              >  1:3279486/1‑150 (MQ=255)
                                                                                                                            gACCTGTGGGTTACGCATCCCTTCCTCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATGGGTGAGAGAGACAATGGACTCGACCATATAGTCAACGAAATGAATCCCTTCAATCGGTTCCCGGAAACGGGCCAGCGCCct                           >  2:456023/1‑150 (MQ=255)
                                                                                                                              ccTGTGGGTTACGCATCCCTTCCGCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATGGGTGAGAGAGACAATGGACTCGACCATATAGTCAACGAAATGAATCCCTTCAATCGGTTCCCGGAAACGGGCCAGCGCCctct                         <  1:2685583/150‑1 (MQ=255)
                                                                                                                               cTGTGGGTTACGCATCCCTTCCGCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATGGGTGAGAGAGACAATGGACTCGACCATATAGTCAACGAAATGAATCCCTTCAATCGGTTCCCGGAAACGGGCCAGCGCCCtctc                        <  1:3986102/150‑1 (MQ=255)
                                                                                                                                tgtgGGTTACGCATCCCTTCCGCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATGGGTGAGAGAGACAATGGACTCGACCATATAGTCAACGAAATGAATCCCTTCAATCGGTTCCCGGAAACGGGCCAGCGCCCTCTcc                       >  2:4880812/1‑150 (MQ=255)
                                                                                                                                   gggTTACGCATCCCTTCCGCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATGGGTGAGAGAGACAATGGACTCGACCATATAGTCAACGAAATGAATCCCTTCAATCGGTTCCCGGAAACGGGCCAGCGCCCTCTCCTgg                    >  1:2636128/1‑150 (MQ=255)
                                                                                                                                      ttACGCATCCCTTCCGCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATGGGTGAGAGAGACAATGGACTCGACCATATAGTCAACGAAATGAATCCCTTCAATCGGTTCCCGGAAACGGGCCAGCGCCCTCTCCTGGTcc                 <  1:1030245/150‑1 (MQ=255)
                                                                                                                                            aTCCCTTCCTCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATGGGTGAGAGAGACAATGGACTCGACCATATAGTCAACGAAATGAATCCCTTCAATCGGTTCCCGGAAACGGGCCAGCGCCCTCTCCTGGTCCTGTAAg           >  2:2994676/1‑150 (MQ=255)
                                                                                                                                             tCCCTTCCTCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATGGGTGAGAGAGACAATGGACTCGACCATATAGTCAACGAAATGAATCCCTTCAATCGGTTCCCGGAAACGGGCCAGCGCCCTCTCCTGGTCCTGTAAGa          >  2:422050/1‑150 (MQ=255)
                                                                                                                                                cTTCCGCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATGGGTGAGAGAGACAATGGACTCGACCATATAGTCAACGAAATGAATCCCTTCAATCGGTTCCCGGAAACGGGCCAGCGCCCTCTCCTGGTCCTGTAAGATaa       <  1:1902321/150‑1 (MQ=255)
                                                                                                                                                  tCCGCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATGGGTGAGAGAGACAATGGACTCGACCATATAGTCAACGAAATGAATCCCTTCAATCGGTTCCCGGAAACGGGCCAGCGCCCTCTCCTGGTCCTGTAAGATAATc     <  1:556295/150‑1 (MQ=255)
                                                                                                                                                   ccGCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATGGGTGAGAGAGACAATGGACTCGACCATATAGTCAACGAAATGAATCCCTTCAATCGGTTCCCGGAAACGGGCCAGCGCCCTCTCCTGGTCCTGTAAGATAATCg    >  2:733952/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
GCAGTAAACGTGAAGTCATTGCCGTGTTGATGTCTGGGCTTATCTCGCCTTTTTGCTGGGCATCACGCATCCGCTGGGCAACAAATTCCGTGATCGTCATATGCTTATTTTTAAGCATGGCGGCGACCTGTGGGTTACGCATCCCTTCCGCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATGGGTGAGAGAGACAATGGACTCGACCATATAGTCAACGAAATGAATCCCTTCAATCGGTTCCCGGAAACGGGCCAGCGCCCTCTCCTGGTCCTGTAAGATAATCGCC  >  CP009273/1690832‑1691130

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑