BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	1,795,267	A→T	33.3%	F521I (TTC→ATC)	thrS ←	threonyl‑tRNA synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	1,795,267	0	A	T	33.3%	389.6 / 311.3	285	F521I (TTC→ATC)	thrS	threonyl‑tRNA synthetase
Reads supporting (aligned to +/- strand): ref base A (104/86); new base T (43/52); total (147/138)
Fisher's exact test for biased strand distribution p-value = 1.34e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.86e-01

Only 100 of 292 total aligned reads displayed.

TTAACACGAATGCCCGCATTTGATAGTTTTTGCGTCAATTCGTTAACGTATTCAGACTGTGAATCGGTAATATTCATGATAACAACCTGAACCGGCGCAAGCCAGGTCGGGAAGAAACCAGCGAACTCTTCGGTCAGGATACCGATGAAACGTTCCATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTACTGTACCGCACTGCCATGCACGATCGAGGCAGTCAT  >  CP009273/1795119‑1795416

ttAACACGAATGCCCGCATTTGATAGTTTTTGCGTCAATTCGTTAACGTATTCAGACTGTGAATCGGTAATATTCATGATAACAACCTGAACCGGCGCAAGCCAGGTCGGGAAGAAACCAGCGAACTCTTCGGTCAGGATACCGATGaaa                                                                                                                                                      <  1:3812188/150‑1 (MQ=255)

ttAACACGAATGCCCGCATTTGATAGTTTTTGCGTCAATTCGTTAACGTATTCAGACTGTGAATCGGTAATATTCATGATAACAACCTGAACCGGCGCAAGCCAGGTCGGGAAGAAACCAGCGAACTCTTCGGTCAGGATACCGATGaaa                                                                                                                                                      >  1:235674/1‑150 (MQ=255)

ttAACACGAATGCCCGCATTTGATAGTTTTTGCGTCAATTCGTTAACGTATTCAGACTGTGAATCGGTAATATTCATGATAACAACCTGAACCGGCGCAAGCCAGGTCGGGAAGAAACCAGCGAACTCTTCGGTCAGGATACCGATGaaa                                                                                                                                                      <  1:2009468/150‑1 (MQ=255)

   acacGAATGCCCGCATTTGATAGTTTTTGCGTCAATTCGTTAACGTATTCAGACTGTGAATCGGTAATATTCATGATAACAACCTGAACCGGCGCAAGCCAGGTCGGGAAGAAACCAGCGAACTCTTCGGTCAGGATACCGATGATACGt                                                                                                                                                   >  1:5051820/1‑150 (MQ=255)

   acacGAATGCCCGCATTTGATAGTTTTTGCGTCAATTCGTTAACGTATTCAGACTGTGAATCGGTAATATTCATGATAACAACCTGAACCGGCGCAAGCCAGGTCGGGAAGAAACCAGCGAACTCTTCGGTCAGGATACCGATGAAACGt                                                                                                                                                   >  1:1320081/1‑150 (MQ=255)

   acacGAATGCCCGCATTTGATAGTTTTTGCGTCAATTCGTTAACGTATTCAGACTGTGAATCGGTAATATTCATGATAACAACCTGAACCGGCGCAAGCCAGGTCGGGAAGAAACCAGCGAACTCTTCGGTCAGGATACCGATGAAACGt                                                                                                                                                   <  1:2735372/150‑1 (MQ=255)

    cacGAATGCCCGCATTTGATAGTTTTTGCGTCAATTCGTTAACGTATTCAGACTGTGAATCGGTAATATTCATGATAACAACCTGAACCGGCGCAAGCCAGGTCGGGAAGAAACCAGCGAACTCTTCGGTCAGGATACCGATGAAACGtt                                                                                                                                                  >  2:2163759/1‑150 (MQ=255)

     acGAATGCCCGCATTTGATAGTTTTTGCGTCAATTCGTTAACGTATTCAGACTGTGAATCGGTAATATTCATGATAACAACCTGAACCGGCGCAAGCCAGGTCGGGAAGAAACCAGCGAACTCTTCGGTCAGGATACCGATGATACGTTc                                                                                                                                                 <  2:892066/150‑1 (MQ=255)

     acGAATGCCCGCATTTGATAGTTTTTGCGTCAATTCGTTAACGTATTCAGACTGTGAATCGGTAATATTCATGATAACAACCTGAACCGGCGCAAGCCAGGTCGGGAAGAAACCAGCGAACTCTTCGGTCAGGATACCGATGATACGTTc                                                                                                                                                 <  2:893206/150‑1 (MQ=255)

         aTGCCCGCATTTGATAGTTTTTGCGTCAATTCGTTAACGTATTCAGACTGTGAATCGGTAATATTCATGATAACAACCTGAACCGGCGCAAGCCAGGTCGGGAAGAAACCAGCGAACTCTTCGGTCAGGATACCGATGATACGTTCCATc                                                                                                                                             <  2:4016178/150‑1 (MQ=255)

         aTGCCCGCATTTGATAGTTTTTGCGTCAATTCGTTAACGTATTCAGACTGTGAATCGGTAATATTCATGATAACAACCTGAACCGGCGCAAGCCAGGTCGGGAAGAAACCAGCGAACTCTTCGGTCAGGATACCGATGAAACGTTCCATc                                                                                                                                             <  1:4160446/150‑1 (MQ=255)

         aTGCCCGCATTTGATAGTTTTTGCGTCAATTCGTTAACGTATTCAGACTGTGAATCGGTAATATTCATGATAACAACCTGAACCGGCGCAAGCCAGGTCGGGAAGAAACCAGCGAACTCTTCGGTCAGGATACCGATGAAACGTTCCATc                                                                                                                                             <  2:4533305/150‑1 (MQ=255)

           gCCCGCATTTGATAGTTTTTGCGTCAATTCGTTAACGTATTCAGACTGTGAATCGGTAATATTCATGATAACAACCTGAACCGGCGCAAGCCAGGTCGGGAAGAAACCAGCGAACTCTTCGGTCAGGATACCGATGAAACGTTCCATCGa                                                                                                                                           <  1:4497554/150‑1 (MQ=255)

             ccGCATTTGATAGTTTTTGCGTCAATTCGTTAACGTATTCAGACTGTGAATCGGTAATATTCATGATAACAACCTGAACCGGCGCAAGCCAGGTCGGGAAGAAACCAGCGAACTCTTCGGTCAGGATACCGATGATACGTTCCATCGAcc                                                                                                                                         >  2:3229194/1‑150 (MQ=255)

               gCATTTGATAGTTTTTGCGTCAATTCGTTAACGTATTCAGACTGTGAATCGGTAATATTCATGATAACAACCTGAACCGGCGCAAGCCAGGTCGGGAAGAAACCAGCGAACTCTTCGGTCAGGATACCGATGAAACGTTCCATCGAcccc                                                                                                                                       <  1:954592/150‑1 (MQ=255)

                cATTTGATAGTTTTTGCGTCAATTCGTTAACGTATTCAGACTGTGAATCGGTAATATTCATGATAACAACCTGAACCGGCGCAAGCCAGGTCGGGAAGAAACCAGCGAACTCTTCGGTCAGGATACCGATGAAACGTTCCATCGACCCCa                                                                                                                                      >  2:3689396/1‑150 (MQ=255)

                 aTTTGATAGTTTTTGCGTCAATTCGTTAACGTATTCAGACTGTGAATCGGTAATATTCATGATAACAACCTGAACCGGCGCAAGCCAGGTCGGGAAGAAACCAGCGAACTCTTCGGTCAGGATACCGATGATACGTTCCATCGACCCCAg                                                                                                                                     <  1:3196050/150‑1 (MQ=255)

                 aTTTGATAGTTTTTGCGTCAATTCGTTAACGTATTCAGACTGTGAATCGGTAATATTCATGATAACAACCTGAACCGGCGCAAGCCAGGTCGGGAAGAAACCAGCGAACTCTTCGGTCAGGATACCGATGAAACGTTCCATCGACCCCAg                                                                                                                                     >  2:4145925/1‑150 (MQ=255)

                  tttGATAGTTTTTGCGTCAATTCGTTAACGTATTCAGACTGTGAATCGGTAATATTCATGATAACAACCTGAACCGGCGCAAGCCAGGTCGGGAAGAAACCAGCGAACTCTTCGGTCAGGATACCGATGATACGTTCCATCGACCCCAGa                                                                                                                                    <  1:1927534/150‑1 (MQ=255)

                     gATAGTTTTTGCGTCAATTCGTTAACGTATTCAGACTGTGAATCGGTAATATTCATGATAACAACCTGAACCGGCGCAAGCCAGGTCGGGAAGAAACCAGCGAACTCTTCGGTCAGGATACCGATGATACGTTCCATCGACCCCAGAAtt                                                                                                                                 >  1:3251494/1‑150 (MQ=255)

                     gATAGTTTTTGCGTCAATTCGTTAACGTATTCAGACTGTGAATCGGTAATATTCATGATAACAACCTGAACCGGCGCAAGCCAGGTCGGGAAGAAACCAGCGAACTCTTCGGTCAGGATACCGATGAAACGTTCCATCGACCCCAGAAtt                                                                                                                                 >  1:4470384/1‑150 (MQ=255)

                        gttttttGCGTCAATTCGTTAACGTATTCAGACTGTGAATCGGTAATATTCATGATAACAACCTGAACCGGCGCAAGCCAGGTCGGGAAGAAACCAGCGAACTCTTCGGTCAGGATACCGATGAAACGTTCCATCGACCCCAGAATTgcg                                                                                                                              <  2:3182081/148‑1 (MQ=255)

                                 gTCAATTCGTTAACGTATTCAGACTGTGAATCGGTAATATTCATGATAACAACCTGAACCGGCGCAAGCCAGGTCGGGAAGAAACCAGCGAACTCTTCGGTCAGGATACCGATGAAACGTTCCATCGACCCCAGAATTGCGCGGTGAATc                                                                                                                     >  2:2495675/1‑150 (MQ=255)

                                     aTTCGTTAACGTATTCAGACTGTGAATCGGTAATATTCATGATAACAACCTGAACCGGCGCAAGCCAGGTCGGGAAGAAACCAGCGAACTCTTCGGTCAGGATACCGATGAAACGTTCCATCGACCCCAGAATTGCGCGGTGAATCATTa                                                                                                                 >  1:5062743/1‑150 (MQ=255)

                                     aTTCGTTAACGTATTCAGACTGTGAATCGGTAATATTCATGATAACAACCTGAACCGGCGCAAGCCAGGTCGGGAAGAAACCAGCGAACTCTTCGGTCAGGATACCGATGAAACGTTCCATCGACCCCAGAATTGCGCGGTGAATCATTa                                                                                                                 <  1:4428242/150‑1 (MQ=255)

                                         gTTAACGTATTCAGACTGTGAATCGGTAATATTCATGATAACAACCTGAACCGGCGCAAGCCAGGTCGGGAAGAAACCAGCGAACTCTTCGGTCAGGATACCGATGAAACGTTCCATCGACCCCAGAATTGCGCGGTGAATCATTACCgg                                                                                                             >  1:1576618/1‑150 (MQ=255)

                                           tAACGTATTCAGACTGTGAATCGGTAATATTCATGATAACAACCTGAACCGGCGCAAGCCAGGTCGGGAAGAAACCAGCGAACTCTTCGGTCAGGATACCGATGAAACGTTCCATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTa                                                                                                           >  1:1120913/1‑150 (MQ=255)

                                            aaCGTATTCAGACTGTGAATCGGTAATATTCATGATAACAACCTGAACCGGCGCAAGCCAGGTCGGGAAGAAACCAGCGAACTCTTCGGTCAGGATACCGATGATACGTTCCATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTAc                                                                                                          >  2:4791217/1‑150 (MQ=255)

                                             aCGTATTCAGACTGTGAATCGGTAATATTCATGATAACAACCTGAACCGGCGCAAGCCAGGTCGGGAAGAAACCAGCGAACTCTTCGGTCAGGATACCGATGAAACGTTCCATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTACt                                                                                                         >  2:4785441/1‑150 (MQ=255)

                                              cGTATTCAGACTGTGAATCGGTAATATTCATGATAACAACCTGAACCGGCGCAAGCCAGGTCGGGAAGAAACCAGCGAACTCTTCGGTCAGGATACCGATGATACGTTCCATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTACtt                                                                                                        <  1:729734/150‑1 (MQ=255)

                                               gTATTCAGACTGTGAATCGGTAATATTCATGATAACAACCTGAACCGGCGCAAGCCAGGTCGGGAAGAAACCAGCGAACTCTTCGGTCAGGATACCGATGAAACGTTCCATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTACttt                                                                                                       >  2:2309591/1‑150 (MQ=255)

                                                tATTCAGACTGTGAATCGGTAATATTCATGATAACAACCTGAACCGGCGCAAGCCAGGTCGGGAAGAAACCAGCGAACTCTTCGGTCAGGATACCGATGATACGTTCCATCGACCCCAGAATTGCGCGGTGAATCATTACCGGAACTTTa                                                                                                      >  2:678317/1‑150 (MQ=255)

                                                tATTCAGACTGTGAATCGGTAATATTCATGATAACAACCTGAACCGGCGCAAGCCAGGTCGGGAAGAAACCAGCGAACTCTTCGGTCAGGATACCGATGAAACGTTCCATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTACTTTa                                                                                                      <  1:4805754/150‑1 (MQ=255)

                                                  ttCAGACTGTGAATCGGTAATATTCATGATAACAACCTGAACCGGCGCAAGCCAGGTCGGGAAGAAACCAGCGAACTCTTCGGTCAGGATACCGATGATACGTTCCATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTACTTTACg                                                                                                    <  2:2762246/150‑1 (MQ=255)

                                                  ttCAGACTGTGAATCGGTAATATTCATGATAACAACCTGAACCGGCGCAAGCCAGGTCGGGAAGAAACCAGCGAACTCTTCGGTCAGGATACCGATGAAACGTTCCATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTACTTTACg                                                                                                    <  2:4609681/150‑1 (MQ=255)

                                                    cAGACTGTGAATCGGTAATATTCATGATAACAACCTGAACCGGCGCAAGCCAGGTCGGGAAGAAACCAGCGAACTCTTCGGTCAGGATACCGATGATACGTTCCATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTACTTTACGtt                                                                                                  <  2:4340321/150‑1 (MQ=255)

                                                    cAGACTGTGAATCGGTAATATTCATGATAACAACCTGAACCGGCGCAAGCCAGGTCGGGAAGAAACCAGCGAACTCTTCGGTCAGGATACCGATGAAACGTTCCATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTACTTTACGtt                                                                                                  >  2:183152/1‑150 (MQ=255)

                                                      gACTGTGAATCGGTAATATTCATGATAACAACCTGAACCGGCGCAAGCCAGGTCGGGAAGAAACCAGCGAACTCTTCGGTCAGGATACCGATGAAACGTTCCATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTACTTTACGTTCa                                                                                                <  1:1466414/150‑1 (MQ=255)

                                                        cTGTGAATCGGTAATATTCATGATAACAACCTGAACCGGCGCAAGCCAGGTCGGGAAGAAACCAGCGAACTCTTCGGTCAGGATACCGATGAAACGTTCCATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTACTTTACGTTCAtt                                                                                              <  2:3366226/150‑1 (MQ=255)

                                                        cTGTGAATCGGTAATATTCATGATAACAACCTGAACCGGCGCAAGCCAGGTCGGGAAGAAACCAGCGAACTCTTCGGTCAGGATACCGATGAAACGTTCCATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTACTTTACGTTCAtt                                                                                              <  2:5065120/150‑1 (MQ=255)

                                                         tgtgAATCGGTAATATTCATGATAACAACCTGAACCGGCGCAAGCCAGGTCGGGAAGAAACCAGCGAACTCTTCGGTCAGGATACCGATGAAACGTTCCATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTg                                                                                             >  1:843216/1‑150 (MQ=255)

                                                          gtgAATCGGTAATATTCATGATAACAACCTGAACCGGCGCAAGCCAGGTCGGGAAGAAACCAGCGAACTCTTCGGTCAGGATACCGATGAAACGTTCCATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGt                                                                                            >  1:2042358/1‑150 (MQ=255)

                                                              aTCGGTAATATTCATGATAACAACCTGAACCGGCGCAAGCCAGGTCGGGAAGAAACCAGCGAACTCTTCGGTCAGGATACCGATGATACGTTCCATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTc                                                                                        <  2:1182493/150‑1 (MQ=255)

                                                              aTCGGTAATATTCATGATAACAACCTGAACCGGCGCAAGCCAGGTCGGGAAGAAACCAGCGAACTCTTCGGTCAGGATACCGATGATACGTTCCATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTc                                                                                        <  1:1639635/150‑1 (MQ=255)

                                                              aTCGGTAATATTCATGATAACAACCTGAACCGGCGCAAGCCAGGTCGGGAAGAAACCAGCGAACTCTTCGGTCAGGATACCGATGAAACGTTCCATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTc                                                                                        <  2:1388382/150‑1 (MQ=255)

                                                               tCGGTAATATTCATGATAACAACCTGAACCGGCGCAAGCCAGGTCGGGAAGAAACCAGCGAACTCTTCGGTCAGGATACCGATGAAACGTTCCATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCg                                                                                       <  2:3035361/150‑1 (MQ=255)

                                                                  gTAATATTCATGATAACAACCTGAACCGGCGCAAGCCAGGTCGGGAAGAAACCAGCGAACTCTTCGGTCAGGATACCGATGAAACGTTCCATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCt                                                                                    >  2:2399622/1‑150 (MQ=255)

                                                                   tAATATTCATGATAACAACCTGAACCGGCGCAAGCCAGGTCGGGAAGAAACCAGCGAACTCTTCGGTCAGGATACCGATGAAACGTTCCATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCTa                                                                                   >  2:697419/1‑150 (MQ=255)

                                                                   tAATATTCATGATAACAACCTGAACCGGCGCAAGCCAGGTCGGGAAGAAACCAGCGAACTCTTCGGTCAGGATACCGATGAAACGTTCCATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCTa                                                                                   <  1:4870782/150‑1 (MQ=255)

                                                                      tatTCATGATAACAACCTGAACCGGCGCAAGCCAGGTCGGGAAGAAACCAGCGAACTCTTCGGTCAGGATACCGATGATACGTTCCATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACAt                                                                                <  2:1186123/150‑1 (MQ=255)

                                                                      tatTCATGATAACAACCTGAACCGGCGCAAGCCAGGTCGGGAAGAAACCAGCGAACTCTTCGGTCAGGATACCGATGATACGGTCCATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACAt                                                                                <  2:4263308/150‑1 (MQ=255)

                                                                           aTGATAACAACCTGAACCGGCGCAAGCCAGGTCGGGAAGAAACCAGCGAACTCTTCGGTCAGGATACCGATGATACGTTCCATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACATaagaa                                                                           >  1:1739662/1‑150 (MQ=255)

                                                                           aTGATAACAACCTGAACCGGCGCAAGCCAGGTCGGGAAGAAACCAGCGAACTCTTCGGTCAGGATACCGATGAAACGTTCCATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACATaagaa                                                                           <  2:1318151/150‑1 (MQ=255)

                                                                            tGATAACAACCTGAACCGGCGCAAGCCAGGTCGGGAAGAAACCAGCGAACTCTTCGGTCAGGATACCGATGATACGTTCCATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACATaagaag                                                                          <  2:2732357/150‑1 (MQ=255)

                                                                            tGATAACAACCTGAACCGGCGCAAGCCAGGTCGGGAAGAAACCAGCGAACTCTTCGGTCAGCATACCGATGAAACGTTCCATCGACCCCAGACTTGCGCGGTGAATCAGAACCGGTACTTTACGTTCATTGTCTTCGCCTACATaagaag                                                                          >  2:1968439/1‑150 (MQ=255)

                                                                                aacaacCTGAACCGGCGCAAGCCAGGTCGGGAAGAAACCAGCGAACTCTTCGGTCAGGATACCGATGAAACGTTCCATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCt                                                                      <  1:4128113/150‑1 (MQ=255)

                                                                                 acaacCTGAACCGGCGCAAGCCAGGTCGGGAAGAAACCAGCGAACTCTTCGGTCAGGATACCGATGAAACGTTCCATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTc                                                                     >  2:4971277/1‑150 (MQ=255)

                                                                                  caacCTGAACCGGCGCAAGCCAGGTCGGGAAGAAACCAGCGAACTCTTCGGTCAGGATACCGATGATACGTTCCATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCa                                                                    >  1:150548/1‑150 (MQ=255)

                                                                                   aacCTGAACCGGCGCAAGCCAGGTCGGGAAGAAACCAGCGAACTCTTCGGTCAGGATACCGATGATACGTTCCATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCag                                                                   >  1:4273437/1‑150 (MQ=255)

                                                                                    acCTGAACCGGCGCAAGCCAGGTCGGGAAGAAACCAGCGAACTCTTCGGTCAGGATACCGATGAAACGTTCCATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCaga                                                                  >  2:2070916/1‑150 (MQ=255)

                                                                                      cTGAACCGGCGCAAGCCAGGTCGGGAAGAAACCAGCGAACTCTTCGGTCAGGATACCGATGATACGTTCCATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCagacg                                                                <  2:2597605/150‑1 (MQ=255)

                                                                                        gAACCGGCGCAAGCCAGGTCGGGAAGAAACCAGCGAACTCTTCGGTCAGGATACCGATGATACGTTCCATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCagacgag                                                              >  1:294928/1‑150 (MQ=255)

                                                                                         aaCCGGCGCAAGCCAGGTCGGGAAGAAACCAGCGAACTCTTCGGTCAGGATACCGATGATACGTTCCATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCagacgaga                                                             >  1:664135/1‑150 (MQ=255)

                                                                                            cGGCGCAAGCCAGGTCGGGAAGAAACCAGCGAACTCTTCGGTCAGGATACCGATGATACGTTCCATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCGACATAAGAAGCGCTCAGACGAGACgg                                                          >  2:3480041/1‑150 (MQ=255)

                                                                                            cGGCGCAAGCCAGGTCGGGAAGAAACCAGCGAACTCTTCGGTCAGGATACCGATGAAACGTTCCATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACgg                                                          >  2:107580/1‑150 (MQ=255)

                                                                                             ggCGCAAGCCAGGTCGGGAAGAAACCAGCGAACTCTTCGGTCAGGATACCGATGATACGTTCCATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGc                                                         >  1:403744/1‑150 (MQ=255)

                                                                                              gcgcAAGCCAGGTCGGGAAGAAACCAGCGAACTCTTCGGTCAGGATACCGATGATACGTTCCATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCa                                                        <  1:3792633/150‑1 (MQ=255)

                                                                                              gcgcAAGCCAGGTCGGGAAGAAACCAGCGAACTCTTCGGTCAGGATACCGATGATACGTTCCATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCa                                                        <  1:2787823/150‑1 (MQ=255)

                                                                                                gcAAGCCAGGTCGGGAAGAAACCAGCGAACTCTTCGGTCAGGATACCGATGATACGTTCCATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCaaa                                                      >  1:2603730/1‑150 (MQ=255)

                                                                                                 cAAGCCAGGTCGGGAAGAAACCAGCGAACTCTTCGGTCAGGATACCGATGAAACGTTCCATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAag                                                     >  2:789174/1‑150 (MQ=255)

                                                                                                    gCCAGGTCGGGAAGAAACCAGCGAACTCTTCGGTCAGGATACCGATGAAACGTTCCATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAgaga                                                  <  2:3580696/150‑1 (MQ=255)

                                                                                                     ccAGGTCGGGAAGAAACCAGCGAACTCTTCGGTCAGGATACCGATGATACGTTCCATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGaa                                                 >  1:4022913/1‑150 (MQ=255)

                                                                                                     ccAGGTCGGGAAGAAACCAGCGAACTCTTCGGTCAGGATACCGATGAAACGTTCCATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGaa                                                 <  1:2366043/150‑1 (MQ=255)

                                                                                                      cAGGTCGGGAAGAAACCAGCGAACTCTTCGGTCAGGATACCGATGAAACGTTCCATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAg                                                >  1:1017992/1‑150 (MQ=255)

                                                                                                       aGGTCGGGAAGAAACCAGCGAACTCTTCGGTCAGGATACCGATGAAACTTTCCATCGACCCCAGAATTGCGCGGTGAATCATAACCGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAga                                               >  2:947136/1‑149 (MQ=255)

                                                                                                       aGGTCGGGAAGAAACCAGCGAACTCTTCGGTCAGGATACCGATGAAACGTTCCATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGt                                               <  1:2449240/150‑1 (MQ=255)

                                                                                                        ggTCGGGAAGAAACCAGCGAACTCTTCGGTCAGGATACCGATGAAACGTTCCATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTc                                              >  1:4961834/1‑150 (MQ=255)

                                                                                                          tCGGGAAGAAACCAGCGAACTCTTCGGTCAGGATACCGATGAAACGTTCCATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCa                                            <  1:1382331/150‑1 (MQ=255)

                                                                                                            gggAAGAAACCAGCGAACTCTTCGGTCAGGATACCGATGAAACGTTCCATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGc                                          >  1:3460018/1‑150 (MQ=255)

                                                                                                              gaagaaACCAGCGAACTCTTCGGTCAGGATACCGATGAAACGTTCCATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGACGTCCAGctg                                        >  1:213466/1‑150 (MQ=255)

                                                                                                                    aCCAGCGAACTCTTCGGTCAGGATACCGATGATACGTTCCATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGctgtactgt                                  >  2:58020/1‑150 (MQ=255)

                                                                                                                        gCGAACTCTTCGGTCAGGATACCGATGAAACGTTCCATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTACTGTACCg                              <  1:3475173/150‑1 (MQ=255)

                                                                                                                          gAACTCTTCGGTCAGGATACCGATGATACGTTCCATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTACTGTACCGCa                            <  2:1732873/150‑1 (MQ=255)

                                                                                                                          gAACTCTTCGGTCAGGATACCGATGAAACGTTCCATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTACTGTACCGCa                            <  1:3094438/150‑1 (MQ=255)

                                                                                                                           aaCTCTTCGGTCAGGATACCGATGAAACGTTCCATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTACTGTACCGCAc                           >  1:1969717/1‑150 (MQ=255)

                                                                                                                            aCTCTTCGGTCAGGATACCGATGAAACGTTCCATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTACTGTACCGCACt                          <  1:4542943/150‑1 (MQ=255)

                                                                                                                               ctTCGGTCAGGATACCGATGATACGTTCCATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTACTGTACCGCACTGcc                       <  2:2907246/150‑1 (MQ=255)

                                                                                                                                ttCGGTCAGGATACCGATGAAACGTTCCATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTACTGTACCGCACTGCCa                      >  2:1508775/1‑150 (MQ=255)

                                                                                                                                   ggTCAGGATACCGATGAAACGTTCCATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTACTGTACCGCACTGCCATGc                   >  2:4870289/1‑150 (MQ=255)

                                                                                                                                       aGGATACCGATGATACGTTCCATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTACTGTACCGCACTGCCATGCACGa               >  2:1768476/1‑150 (MQ=255)

                                                                                                                                        ggATACCGATGAAACGTTCCATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTACTGTACCGCACTGCCATGCACGAt              >  1:1164784/1‑150 (MQ=255)

                                                                                                                                           tACCGATGAAACGTTCCATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTACTGTACCGCACTGCCATGCACGATCGa           <  2:1176392/150‑1 (MQ=255)

                                                                                                                                             ccGATGAAACGTTCCATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTACTGTACCGCACTGCCATGCACGATCGAgg         <  2:2230590/150‑1 (MQ=255)

                                                                                                                                             ccGATGAAACGTTCCATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTACTGTACCGCACTGCCATGCACGATCGAgg         <  2:2230485/150‑1 (MQ=255)

                                                                                                                                              cGATGAAACGTTCCATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTACTGTACCGCACTGCCATGCACGATCGAGGc        >  2:4927134/1‑150 (MQ=255)

                                                                                                                                              cGATGAAACGTTCCATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTACTGTACCGCACTGCCATGCACGATCGAGGc        >  2:3718000/1‑150 (MQ=255)

                                                                                                                                               gATGAAACGTTCCATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTACTTTACTTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTACTGTACCGCACTGCCATGCACGATCGAGGCa       <  1:458134/150‑1 (MQ=255)

                                                                                                                                                  gAAACGTTCCATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTACTGTACCGCACTGCCATGCACGATCGAGGCAGTc    >  1:59846/1‑150 (MQ=255)

                                                                                                                                                  gAAACGTTCCATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTACTGTACCGCACTGCCATGCACGATCGAGGCAGTc    >  2:4398979/1‑150 (MQ=255)

                                                                                                                                                    aaCGTTCCATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTACTGTACCGCACTGCCATGCACGATCGAGGCAGTCAt  >  2:3924766/1‑150 (MQ=255)

TTAACACGAATGCCCGCATTTGATAGTTTTTGCGTCAATTCGTTAACGTATTCAGACTGTGAATCGGTAATATTCATGATAACAACCTGAACCGGCGCAAGCCAGGTCGGGAAGAAACCAGCGAACTCTTCGGTCAGGATACCGATGAAACGTTCCATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTACTGTACCGCACTGCCATGCACGATCGAGGCAGTCAT  >  CP009273/1795119‑1795416

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`