BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	1,848,031	C→T	7.2%	G69D (GGT→GAT)	ydjE ←	putative transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	1,848,031	0	C	T	7.2%	1143.0 / 54.8	319	G69D (GGT→GAT)	ydjE	putative transporter
Reads supporting (aligned to +/- strand): ref base C (131/163); new base T (12/11); total (143/176)
Fisher's exact test for biased strand distribution p-value = 5.19e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 328 total aligned reads displayed.

GGAAGCGAAAGAAGATGAGCCAGTACATATCAGGGACAAAAGCGGCCCCTGTTGCAGCAATACCGACGATGAGAAGATTTATGCGAAACGCCCTGCGCCGCCCAAAGTAGTCACCAATAAACCCACCAGTAAGTGAGCCGATGAAATAACCAAACATTAATGCCGAGGTAAAGGCGGCATTGAGGAAATTATTTGACCATCCATTGCTTACCAGCTTTGCTAAGACGACATTACCGGAGTAACTCAAAAACCCTGTTAATAACAGACTAAAGCTTATAATACCAAATATACGATAATG  >  CP009273/1847882‑1848179

ggAAGCGAAAGAAGATGAGCCAGTACATATCAGGGACAAAAGCGGCCCCTGTTGCAGCAATACCGACGATGAGAAGATTTATGCGAAACGCCCTGCGCCGCCCAAAGTAGTCACCAATAAACCCACCAGTAAGTGAGCCGATGAAATAAc                                                                                                                                                      >  2:3727933/1‑150 (MQ=255)

ggAAGCGAAAGAAGATGAGCCAGTACATATCAGGGACAAAAGCGGCCCCTGTTGCAGCAATACCGACGATGAGAAGATTTATGCGAAACGCCCTGCGCCGCCCAAAGTAGTCACCAATAAACCCACCAGTAAGTGAGCCGATGAAATAAc                                                                                                                                                      <  2:202881/150‑1 (MQ=255)

 gAAGCGAAAGAAGATGAGCCAGTACATATCAGGGACAAAAGCGGCCCCTGTTGCAGCAATACCGACGATGAGAAGATTTATGCGAAACGCCCTGCGCCGCCCAAAGTAGTCACCAATAAACCCACCAGTAAGTGAGCCGATGAAATAAcc                                                                                                                                                     >  2:2444878/1‑150 (MQ=255)

 gAAGCGAAAGAAGATGAGCCAGTACATATCAGGGACAAAAGCGGCCCCTGTTGCAGCAATACCGACGATGAGAAGATTTATGCGAAACGCCCTGCGCCGCCCAAAGTAGTCACCAATAAACCCACCAGTAAGTGAGCCGATGAAATAAcc                                                                                                                                                     >  2:3930547/1‑150 (MQ=255)

  aaGCGAAAGAAGATGAGCCAGTACATATCAGGGACAAAAGCGGCCCCTGTTGCAGCAATACCGACGATGAGAAGATTTATGCGAAACGCCCTGCGCCGCCCAAAGTAGTCACCAATAAACCCACCAGTAAGTGAGCCGATGAAATAACCa                                                                                                                                                    <  1:3489312/150‑1 (MQ=255)

       aaaGAAGATGAGCCAGTACATATCAGGGACAAAAGCGGCCCCTGTTGCAGCAATACCGACGATGAGAAGATTTATGCGAAACGCCCTGCGCCGCCCAAAGTAGTCACCAATAAACCCACCAGTAAGTGAGCCGATGAAATAACCAAACAt                                                                                                                                               >  1:3867216/1‑150 (MQ=255)

        aagaagATGAGCCAGTACATATCAGGGACAAAAGCGGCCCCTGTTGCAGCAATACCGACGATGAGAAGATTTATGCGAAACGCCCTGCGCCGCCCAAAGTAGTCACCAATAAACCCACCAGTAAGTGAGCCGATGAAATAACCAAACAtt                                                                                                                                              >  1:5097456/1‑150 (MQ=255)

         agaagaTGAGCCAGTACATATCAGGGACAAAAGCGGCCCCTGTTGCAGCAATACCGACGATGAGAAGATTTATGCGAAACGCCCTGCGCCGCCCAAAGTAGTCACCAATAAACCCACCAGTAAGTGAGCCGATGAAATAACCAAACATTa                                                                                                                                             >  1:906316/1‑150 (MQ=255)

          gaagaTGAGCCAGTACATATCAGGGACAAAAGCGGCCCCTGTTGCAGCAATACCGACGATGAGAAGATTTATGCGAAACGCCCTGCGCCGCCCAAAGTAGTCACCAATAAACCCACCAGTAAGTGAGCCGATGAAATAATCAAACATTaa                                                                                                                                            <  1:3380036/150‑1 (MQ=255)

           aagaTGAGCCAGTACATATCAGGGACAAAAGCGGCCCCTGTTGCAGCAATACCGACGATGAGAAGATTTATGCGAAACGCCCTGCGCCGCCCAAAGTAGTCACCAATAAACCCACCAGTAAGTGAGCCGATGAAATAATCAAACATTAAt                                                                                                                                           >  1:3608841/1‑150 (MQ=255)

            agaTGAGCCAGTACATATCAGGGACAAAAGCGGCCCCTGTTGCAGCAATACCGACGATGAGAAGATTTATGCGAAACGCCCTGCGCCGCCCAAAGTAGTCACCAATAAACCCACCAGTAAGTGAGCCGATGAAATAACCAAACATTAATg                                                                                                                                          >  1:3097733/1‑150 (MQ=255)

               tGAGCCAGTACATATCAGGGACAAAAGCGGCCCCTGTTGCAGCAATACCGACGATGAGAAGATTTATGCGAAACGCCCTGCGCCGCCCAAAGTAGTCACCAATAAACCCACCAGTAAGTGAGCCGATGAAATAACCAAACATTAATGCCg                                                                                                                                       <  1:1301552/150‑1 (MQ=255)

                   ccAGTACATATCAGGGACAAAAGCGGCCCCTGTTGCAGCAATACCGACGATGAGAAGATTTATGCGAAACGCCCTGCGCCGCCCAAAGTAGTCACCAATAAACCCACCAGTAAGTGAGCCGATGAAATAACCAAACATTAATGCCGAGGt                                                                                                                                   <  1:2164487/150‑1 (MQ=255)

                   ccAGTACATATCAGGGACAAAAGCGGCCCCTGTTGCAGCAATACCGACGATGAGAAGATTTATGCGAAACGCCCTGCGCCGCCCAAAGTAGTCACCAATAAACCCACCAGTAAGTGAGCCGATGAAATAACCAAACATTAATGCCGAGGt                                                                                                                                   <  2:787220/150‑1 (MQ=255)

                    cAGTACATATCAGGGACAAAAGCGGCCCCTGTTGCAGCAATACCGACGATGAGAAGATTTATGCGAAACGCCCTGCGCCGCCCAAAGTAGTCACCAATAAACCCACCAGTAAGTGAGCCGATGAAATAACCAAACATTAATGCCGAGGTa                                                                                                                                  <  1:919306/150‑1 (MQ=255)

                    cAGTACATATCAGGGACAAAAGCGGCCCCTGTTGCAGCAATACCGACGATGAGAAGATTTATGCGAAACGCCCTGCGCCGCCCAAAGTAGTCACCAATAAACCCACCAGTAAGTGAGCCGATGAAATAACCAAACATTAATGCCGAGGTa                                                                                                                                  <  2:2887116/150‑1 (MQ=255)

                        aCATATCAGGGACAAAAGCGGCCCCTGTTGCAGCAATACCGACGATGAGAAGATTTATGCGAAACGCCCTGCGCCGCCCAAAGTAGTCACCAATAAACCCACCAGTAAGTGAGCCGATGAAATAACCAAACATTAATGCCGAGGTAAAgg                                                                                                                              >  2:1826954/1‑150 (MQ=255)

                        aCATATCAGGGACAAAAGCGGCCCCTGTTGCAGCAATACCGACGATGAGAAGATTTATGCGAAACGCCCTGCGCCGCCCAAAGTAGTCACCAATAAACCCACCAGTAAGTGAGCCGATGAAATAACCAAACATTAATGCCGAGGTAAAgg                                                                                                                              >  2:1501808/1‑150 (MQ=255)

                         cATATCAGGGACAAAAGCGGCCCCTGTTGCAGCAATACCGACGATGAGAAGATTTATGCGAAACGCCCTGCGCCGCCCAAAGTAGTCACCAATAAACCCACCAGTAAGTGAGCCGATGAAATAACCAAACATTAATGCCGAGGTAAAggc                                                                                                                             <  1:4537661/150‑1 (MQ=255)

                         cATATCAGGGACAAAAGCGGCCCCTGTTGCAGCAATACCGACGATGAGAAGATTTATGCGAAACGCCCTGCGCCGCCCAAAGTAGTCACCAATAAACCCACCAGTAAGTGAGCCGATGAAATAACCAAACATTAATGCCGAGGTAAAggc                                                                                                                             >  1:2996257/1‑150 (MQ=255)

                         cATATCAGGGACAAAAGCGGCCCCTGTTGCAGCAATACCGACGATGAGAAGATTTATGCGAAACGCCCTGCGCCGCCCAAAGTAGTCACCAATAAACCCACCAGTAAGTGAGCCGATGAAATAACCAAACATTAATGCCGAGGTAAAggc                                                                                                                             >  1:4484461/1‑150 (MQ=255)

                          atatCAGGGACAAAAGCGGCCCCTGTTGCAGCAATACCGACGATGAGAAGATTTATGCGAAACGCCCTGCGCCGCCCAAAGTAGTCACCAATAAACCCACCAGTAAGTGAGCCGATGAAATAACCAAACATTAATGCCGAGGTAAAggcg                                                                                                                            >  1:4076702/1‑150 (MQ=255)

                            atCAGGGACAAAAGCGGCCCCTGTTGCAGCAATACCGACGATGAGAAGATTTATGCGAAACGCCCTGCGCCGCCCAAAGTAGTCACCAATAAACCCACCAGTAAGTGAGCCGATGAAATAACCAAACATTAATGCCGAGGTAAAggcggc                                                                                                                          <  2:728940/150‑1 (MQ=255)

                              cAGGGACAAAAGCGGCCCCTGTTGCAGCAATACCGACGATGAGAAGATTTATGCGAAACGCCCTGCGCCGCCCAAAGTAGTCACCAATAAACCCACCAGTAAGTGAGCCGATGAAATAACCAAACATTAATGCCGAGGTAAAGGCGGCAt                                                                                                                        >  2:1446348/1‑150 (MQ=255)

                               aGGGACAAAAGCGGCCCCTGTTGCAGCAATACCGACGATGAGAAGATTTATGCGAAACGCCCTGCGCCGCCCACAGTAGTCACCAATAAACCCACCAGTAAGTGAGCCGATGAAATAACCAAACATTAATGCCGAGGTAAAGGCGGCAtt                                                                                                                       <  2:1795309/150‑1 (MQ=255)

                               aGGGACAAAAGCGGCCCCTGTTGCAGCAATACCGACGATGAGAAGATTTATGCGAAACGCCCTGCGCCGCCCAAAGTAGTCACCAATAAACCCACCAGTAAGTGAGCCGATGAAATAACCAAACATTAATGCCGAGGTAAAGGCGGCAtt                                                                                                                       >  2:4655067/1‑150 (MQ=255)

                               aGGGACAAAAGCGGCCCCTGTTGCAGCAATACCGACGATGAGAAGATTTATGCGAAACGCCCTGCGCCGCCCAAAGTAGTCACCAATAAACCCACCAGTAAGTGAGCCGATGAAATAACCAAACATTAATGCCGAGGTAAAGGCGGCAtt                                                                                                                       >  2:3625562/1‑150 (MQ=255)

                                       aaGCGGCCCCTGTTGCAGCAATACCGACGATGAGAAGATTTATGCGAAACGCCCTGCGCCGCCCAAAGTAGTCACCAATAAACCCACCAGTAAGTGAGCCGATGAAATAACCAAACATTAATGCCGAGGTAAAGGCGGCATTGAGGaaag                                                                                                               >  2:1957207/1‑149 (MQ=255)

                                          ctgCCACTGTTGCATCAATACCGAAGATGAGAAGATTTATGCGAAACGCCCTGCGCCTCCGAAAGTAGTCACCATTTCACCCACCAGTAAGTGAGCCGATGAAATAACCAAACATTAATGCCGAGGTTAAGGGGGGATTGTGTAtattat                                                                                                            <  2:3531650/148‑1 (MQ=255)

                                          cGGCCCCTGTTGCAGCAATACCGACGATGAGAAGATTTATGCGAAACGCCCTGCGCCGCCCAAAGTAGTCACCAATAAACCCACCAGTAAGTGAGCCGATGAAATAACCAAACATTAATGCCGAGGTAAAGGCGGCATTGAGGAAattat                                                                                                            <  2:1901407/150‑1 (MQ=255)

                                           ggCCCCTGTTGCAGCAATACCGACGATGAGAAGATTTATGCGAAACGCCCTGCGCCGCCCAAAGTAGTCACCAATAAACCCACCAGTAAGTGAGCCGATGAAATAACCAAACATTAATGCCGAGGTATAGGCGGCATTGAGGAAattatt                                                                                                           <  2:5076617/150‑1 (MQ=255)

                                           ggCCCCTGTTGCAGCAATACCGACGATGAGAAGATTTATGCGAAACGCCCTGCGCCGCCCAAAGTAGTCACCAATAAACCCACCAGTAAGTGAGCCGATGAAATAACCAAACATTAATGCCGAGGTAAAGGCGGCATTGAGGAAattatt                                                                                                           <  2:329349/150‑1 (MQ=255)

                                           ggCCCCTGTTGCAGCAATACCGACGATGAGAAGATTTATGCGAAACGCCCTGCGCCGCCCAAAGTAGTCACCAATAAACCCACCAGTAAGTGAGCCGATGAAATAACCAAACATTAATGCCGAGGTAAAGGCGGCATTGAGGAAattatt                                                                                                           >  2:859153/1‑150 (MQ=255)

                                           ggCCCCTGTTGCAGCAAGACCGACGATGAGAAGATTTATGCGAAACGCCCTGCGCCGCCCAAAGTAGTCACCAATAAACCCACCAGTAAGTGAGCCGATGAAATAACCAAACATTAATGCCGAGGTAAAGGCGGCATTGAGGAAattatt                                                                                                           <  2:4157758/150‑1 (MQ=255)

                                            gCCCCTGTTGCAGCAATACCGACGATGAGAAGATTTATGCGAAACGCCCTGCGCCGCCCAAAGTAGTCACCAATAAACCCACCAGTAAGTGAGCCGATGAAATAACCAAACATTAATGCCGAGGTAAAGGCGGCATTGAGGAAATTAttt                                                                                                          >  1:3118250/1‑150 (MQ=255)

                                               ccTGTTGCAGCAATACCGACGATGAGAAGATTTATGCGAAACGCCCTGCGCCGCCCAAAGTAGTCACCAATAAACCCACCAGTAAGTGAGCCGATGAAATAACCAAACATTAATGCCGAGGTAAAGGCGGCATTGAGGAAATTATTTGAc                                                                                                       <  1:1227601/150‑1 (MQ=255)

                                                 tGTTGCAGCAATACCGACGATGAGAAGATTTATGCGAAACGCCCTGCGCCGCCCAAAGTAGTCACCAATAAACCCACCAGTAAGTGAGCCGATGAAATAATCAAACATTAATGCCGAGGTAAAGGCGGCATTGAGGAAATTATTTGAcca                                                                                                     >  2:107954/1‑150 (MQ=255)

                                                   ttGCAGCAATACCGACGATGAGAAGATTTATGCGAAACGCCCTGCGCCGCCCAAAGTAGTCACCAATAAACCCACCAGTAAGTGAGCCGATGAAATAATCAAACATTAATGCCGAGGTAAAGGCGGCATTGAGGAAATTATTTGAccatc                                                                                                   <  2:3252455/150‑1 (MQ=255)

                                                    tGCAGCAATACCGACGATGAGAAGATTTATGCGAAACGCCCTGCGCCGCCCAAAGTAGTCACCAATAAACCCACCAGTAAGTGAGCCGATGAAATAACCAAACATTAATGCCGAGGTAAAGGCGGCATTGAGGAAATTATTTGAccatcc                                                                                                  <  1:4225756/150‑1 (MQ=255)

                                                    tGCAGCAATACCGACGATGAGAAGATTTATGCGAAACGCCCTGCGCCGCCCAAAGTAGTCACCAATAAACCCACCAGTAAGTGAGCCGATGAAATAACCAAACATTAATGCCGAGGTAAAGGCGGCATTGAGGAAATTATTTGAccatcc                                                                                                  <  1:2326519/150‑1 (MQ=255)

                                                       agcaATACCGACGATGAGAAGATTTATGCGAAACGCCCTGCGCCGCCCAAAGTAGTCACCAATAAACCCACCAGTAAGTGAGCCGATGAAATAACCAAACATTACTGCCGAGGTAAAGGCGGCATTGAGGAAATTATTTGACCATCCAtt                                                                                               <  1:2695216/150‑1 (MQ=255)

                                                       agcaATACCGACGATGAGAAGATTTATGCGAAACGCCCTGCGCCGCCCAAAGTAGTCACCAATAAACCCACCAGTAAGTGAGCCGATGAAATAACCAAACATTAATGCCGAGGTAAAGGCGGCATTGAGGAAATTATTTGACCATCCAtt                                                                                               <  1:4357021/150‑1 (MQ=255)

                                                         caATACCGACGATGAGAAGATTTATGCGAAACGCCCTGCGCCGCCCAAAGTAGTCACCAATAAACCCACCAGTAAGTGAGCCGATGAAATAACCAAACATTAATGCCGAGGTAAAGGCGGCATTGAGGAAATTATTTGACCATCCATTGc                                                                                             <  1:3936560/150‑1 (MQ=255)

                                                         caATACCGACGATGAGAAGATTTATGCGAAACGCCCTGCGCCGCCCAAAGTAGTCACCAATAAACCCACCAGTAAGTGAGCCGATGAAATAACCAAACATTAATGCCGAGGTAAAGGCGGCATTGAGGAAATTATTTGACCATCCATTGc                                                                                             <  2:5103770/150‑1 (MQ=255)

                                                          aaTACCGACGATGAGAAGATTTATGCGAAACGCCCTGCGCCGCCCAAAGTAGTCACCAATAAACCCACCAGTAAGTGAGCCGATGAAATAACCAAACATTAATGCCGAGGTAAAGGCGGCATTGAGGAAATTATTTGACCATCCATTGCt                                                                                            <  1:10904/150‑1 (MQ=255)

                                                          aaTACCGACGATGAGAAGATTTATGCGAAACGCCCTGCGCCGCCCAAAGTAGTCACCAATAAACCCACCAGTAAGTGAGCCGATGAAATAACCAAACATTAATGCCGAGGTAAAGGCGGCATTGAGGAAATTATTTGACCATCCATTGCt                                                                                            >  2:4354551/1‑150 (MQ=255)

                                                            tACCGACGATGAGAAGATTTATGCGAAACGCCCTGCGCCGCCCAAAGTAGTCACCAATAAACCCACCAGTAAGTGAGCCGATGAAATAACCAAACATTAATGCCGAGGTAAAGGCGGCATTGAGGAAATTATTTGACCATCCATTGCTTa                                                                                          <  1:659709/150‑1 (MQ=255)

                                                              ccgtctaTGAGAAGTTTTATGCGAAAAGCCCTGCGGCGCCCATTGTAGTCAGCAATAAACCCACCAGTAAGTGAGCCGATGAAATAACCAAACATTTATGCCGAGGTAAAGGCGGCATTGAGGAAATTTTTTGACAATACATTGCTTAcc                                                                                        <  2:403155/144‑1 (MQ=255)

                                                              ccGACGATGAGAAGATTTATGCGAAACGCCCTGCGCCGCCCAAAGTAGTCACCAATAAACCCACCAGTAAGTGAGCCGATGAAATAACCAAACATTAATGCCGAGGTAAAGGCGGCATTGAGGAAATTATTTGACCATCCATTGCTTAcc                                                                                        <  2:2500507/150‑1 (MQ=255)

                                                              ccGACGATGAGAAGATTGATGCGAAACGCCCTGCGCCGCCCAAAGTAGTCACCAATACACCCACCAGTAAGTGAGCCGATGAAATAACCAAACATTAATGCCGAGGTAAAGGCGGCATTGAGGAAATTATTTGACCATCCATTGCTTAcc                                                                                        <  2:631196/150‑1 (MQ=255)

                                                               cgacgaTGAGAAGATTTATGCGAAACGCCCTGCGCCGCCCAAAGTAGTCACCAATAAACCCACCAGTAAGTGAGCCGATGAAATAATCAAACAGTAATGCCGAGGTAAAGGCGGCATTGACGAAATTATTTGACCATCCATTGCTTACCa                                                                                       >  1:5144203/1‑150 (MQ=255)

                                                               cgacgaTGAGAAGATTTATGCGAAACGCCCTGCGCCGCCCAAAGTAGTCACCAATAAACCCACCAGTAAGTGAGCCGATGAAATAACCAAACATTAATGCCGAGGTAAAGGCGGCATTGAGGAAATTATTTGACCATCCATTGCTTACCa                                                                                       >  2:2696904/1‑150 (MQ=255)

                                                                 acgaTGAGAAGATTTATGCGAAACGCCCTGCGCCGCCCAAAGTAGTCACCAATAAACCCACCAGTAAGTGAGCCGATGAAATAACCAAACATTAATGCCGAGGTAAAGGCGGCATTGAGGAAATTATTTGACCATCCATTGCTTACCAGc                                                                                     <  2:4348584/150‑1 (MQ=255)

                                                                      gagaAGATTTATGCGAAACGCCCTGCGCCGCCCAAAGTAGTCACCAATAAACCCACCAGTAAGTGAGCCGATGAAATAACCAAACATTAATGCCGAGGTAAAGGCGGCATTGAGGAAATTATTTGACCATCCATTGCTTACCAGCTTTGc                                                                                <  2:4909052/150‑1 (MQ=255)

                                                                        gaagaTTTATGCGAAACGCCCTGCGCCGCCCAAAGTAGTCACCAATAAACCCACCAGTAAGTGAGCCGATGAAATAACCAAACATTAATGCCGAGGTAAAGGCGGCATTGAGGAAATTATTAGACCATCCATTGCTTACCAGCTTTGCTa                                                                              <  2:4721581/150‑1 (MQ=255)

                                                                           gaTTTATGCGAAACGCCCTGCGCCGCCCAAAGTAGTCACCAATAAACCCACCAGTAAGTGAGCCGATGAAATAACCAAACATTAATGCCGAGGTAAAGGCGGCATTGAGGAAATTATTTGACCATCCATTGCTTACCAGCTTTGCTAAga                                                                           >  2:2846886/1‑150 (MQ=255)

                                                                            aTTTATGCGAAACGCCCTGCGCCGCCCAAAGTAGTCACCAATAAACCCACCAGTAAGTGAGCCGATGAAATAACCAAACATTAATGCCGAGGTAAAGGCGGCATTGAGGAAATTATTTGACCATCCATTGCTTACCAGCTTTGCTAAgac                                                                          <  1:4820936/150‑1 (MQ=255)

                                                                               tATGCGAAACGCCCTGCGCCGCCCAAAGTAGTCACCAATAAACCCACCAGTAAGTGAGCCGATGAAATAACCAAACATTAATGCCGAGGTAAAGGCGGCATTGAGGAAATTATTTGACCATCCATTGCTTACCAGCTTTGCTAAgacgac                                                                       >  2:2588142/1‑150 (MQ=255)

                                                                                 tGCGAAACGCCCTGCGCCGCCCAAAGTAGTCACCAATAAACCCACCAGTAAGTGAGCCGATGAAATAACCAAACATTAATGCCGAGGTAAAGGCGGCATTGAGGAAATTATTTGACCATCCATTGCTTACCAGCTTTGCTAAGACGACAt                                                                     <  1:1979233/150‑1 (MQ=255)

                                                                                     aaaCGCCCTGCGCCTCCCAAAGTAGTCACCAATAATCCCACAATTAAGTTAGACGATTAAATAAAAAAACATTAATGCCGAGGTAAAGGCGGCATTGAGGAAATTTTTTTACCATCCATTGCTTACCAGCTTTGCTAAGACTACATTAcc                                                                 <  2:4598419/150‑1 (MQ=255)

                                                                                     aaaCGCCCTGCGCCGCCCAAAGTAGTCACCAATAAACCCACCAGTAAGTGAGCCGATGAAATAACCAAACATTAATGCCGAGGTAAAGGCGGCATTGAGGAAATTATTTGACCATCCATTGCTTACCAGCTTTGCTAAGACGACATTAcc                                                                 <  2:906316/150‑1 (MQ=255)

                                                                                     aaaCGCCCTGCGCCGCCCAAAGTAGTCACCAATAAACCCACCAGTAAGTGAGCCGATGAAATAACCAAACATTAATGCCGAGGTAAAGGCGGCATTGAGGAAATTATTTGACCATCCATTGCTTACCAGCTTTGCTAAGACGACATTAcc                                                                 <  1:2241064/150‑1 (MQ=255)

                                                                                      aaCGCCCTGCGCCGCCCAAAGTAGTCACCAATAAACCCACCAGTAAGTGAGCCGATGAAATAACCAAACATTAATGCCGAGGTAAAGGCGGCATTGAGGAAATTATTTGACCATCCATTGCTTACCAGCTTTGCTAAGACGACATTACCg                                                                >  1:1736861/1‑150 (MQ=255)

                                                                                       aCGCCCTGCGCCGCCCAAAGTAGTCACCAATAAACCCACCAGTAAGTGAGCCGATGAAATAACCAAACATTAATGCCGAGGTAAAGGCGGCATTGAGGAAATTATTTGACCATCCATTGCTTACCAGCTTTGCTAAGACGACATTACCgg                                                               >  2:2239155/1‑150 (MQ=255)

                                                                                        cGCCCTGCGCCGCCCAAAGTAGTCACCAATAAACCCACCAGTAAGTGAGCCGATGAAATAACCAAACATTAATGCCGAGGTAAAGGCGGCATTGAGGAAATTATTTGACCATCCATTGCTTACCAGCTTTGCTAAGACGACATTACCGGa                                                              >  2:3405119/1‑150 (MQ=255)

                                                                                          cccTGCGCCGCCCAAAGTAGTCACCAATAAACCCACCAGTAAGTGAGCCGATGAAATAACCAAACATTAATGCCGAGGTAAAGGCGGCATTGAGGAATTTATTTGACAATCCATTGCTTACCAGCTTTGCTAAGACGACATTACCGGAGt                                                            <  2:3471169/150‑1 (MQ=255)

                                                                                           ccTGCGCCGCCCAAAGTAGTCACCAATAAACCCACCAGTAAGTGAGCCGATGAAATAACCAAACATTAATGCCGAGGTAAAGGCGGCATTGAGGAAATTATTTGACCATCCATTGCTTACCAGCTTTGCTAAGACGACATTACCGGAGTa                                                           <  2:120113/150‑1 (MQ=255)

                                                                                               cgccgcCCAAAGTAGTCACCAATAAACCCACCAGTAAGTGAGCCGATGAAATAACCAAACATTAATGCCGAGGTAAAGGCGGCATTGAGGAAATTATTTGACCATCCATTGCTTACCAGCTTTGCTAAGACGACATTACCGGAGTAACTc                                                       <  2:1733896/150‑1 (MQ=255)

                                                                                                gccgccCAAAGTAGTCACCAATAAACCCACCAGTAAGTGAGCCGATGAAATAATCAAACATTAATGCCGAGGTAAAGGCGGCATTGAGGACATTATTTGACCATCCATTGCTTACCAGCTTTGCTAAGACGACATTACCGGAGTACCTCa                                                      >  1:2880317/1‑150 (MQ=255)

                                                                                                gccgccCAAAGTAGTCACCAATAAACCCACCAGTAAGTGAGCCGATGAAATAACCAAACATTAATGCCGAGGTAAAGGCGGCATTGAGGAAATTATTTGACCATCCATTGCTTACCAGCTTTGCTAAGACGACATTACCGGAGTAACTCa                                                      <  1:1313234/150‑1 (MQ=255)

                                                                                                 ccgccCAAAGTAGTCACCAATAAACCCACCAGTAAGTGAGCCGATGAAATAACCAAACATTAATGCCGAGGTAAAGGCGGCATTGAGGAAATTATTTGACCATCCATTGCTTACCAGCTTTGCTAAGACGACATTACCGGAGTAACTCaa                                                     <  1:2028461/150‑1 (MQ=255)

                                                                                                     ccAAAGTAGTCACCAATAAACCCACCAGTAAGTGAGCCGATGAAATAACCAAACATTAATGCCGAGGTAAAGGCGGCATTGAGGAAATTATTTGACCATCCATTGCTTACCAGCTTTGCTAAGACGACATTACCGGAGTAACTCAAAAAc                                                 <  1:267902/150‑1 (MQ=255)

                                                                                                       aaaGTAGTCACCAATAAACCCACCAGTAAGTGAGCCGATGAAATAACCAAACATTAATGCCGAGGTAAAGGCGGCATTGAGGAAATTATTTGACCATCCATTGCTTACCAGCTTTGCTAAGACGACATTACCGGAGTAACTCAAAAAccc                                               >  1:2428574/1‑150 (MQ=255)

                                                                                                         agtagtCACCAATAAACCCACCAGTAAGTGAGCCGATGAAATAACCAAACATTAATGCCGAGGTAAAGGCGGCATTGAGGAAATTATTTGACCATCCATTGCTTACCAGCTTTGCTAAGACGACATTACCGGAGTAACTCAAAAACCCTg                                             >  1:634506/1‑150 (MQ=255)

                                                                                                          tttctCACAAATAAACACAACAGTAAGTGAGCCTATGCAATATACAATCATTACTGACGAGTTTAATGAGTCATTGATGAAATTATTTGACCATCCATTTCTTACATTGTTTGCTATGTCGACATTACCGGAGTAACTCAAAAACCCTGt                                            <  2:2650718/146‑1 (MQ=255)

                                                                                                            agtCACCAATAAACCCACCAGTAAGTGAGCCGATGAAATAACCAAACATTAATGCCGAGGTAAAGGCGGCATTGAGGAAATTATTTGACCATCCATTGCTTACCAGCTTTGCTAAGACGACATTACCGGAGTAACTCAAAAACCCTGTta                                          <  1:1891230/150‑1 (MQ=255)

                                                                                                               cACCAATAAACCCACCAGTAAGTGAGCCGATGAAATAACCAAACATTAATGCCGAGGTAAAGGCGGCATTGAGGAAATTATTTGACCATCCATTGCTTACCAGCTTTGCTAAGACGACATTACCGGAGTAACTCAAAAACCCTGTtaata                                       >  2:3917096/1‑150 (MQ=255)

                                                                                                                aCCAATACACCCACCAGTAAGTGAGCCGATGAAATAACCAAACATTAATGCCGAGGTAAAGGCGGCATTGAGGAAATTATTTGACCATCCATTTCTTACCAGCTTTGCTAAGACGACATTACCGGAGTAACTCAAAAACCCTGTtaataa                                      <  2:4043746/150‑1 (MQ=255)

                                                                                                                    aTAAACCCACCAGTAAGTGAGCCGATGAAATAACCAAACATTAATGCCGAGGTAAAGGCGGCAGTGAGGAAATTATTTGACCATCCATTGCTTACCAGCTTTGCTAAGACGACATTACCGGAGTAACTCAAAAACCCTGTTAATAACAGa                                  >  2:3615153/1‑150 (MQ=255)

                                                                                                                       aaCCCACCAGTAAGTGAGCCGATGAAATAACCAAACATTAATGCCGAGGTAAAGGCGGCATTGAGGAAATTATTTGACCATCCATTGCTTACCAGCTTTGCTAAGACGACATTACCGGAGTAACTCAAAAACCCTGTTCATAACAGACTa                               >  1:3812626/1‑150 (MQ=255)

                                                                                                                         cccACCAGTAAGTGAGCCGATGAAATAACCAAACATTAATGCCGAGGTAAAGGCGGCATTGAGGAAATTATTTGACCATCCATTGCTTACCAGCTTTGCTAAGACGACATTACCGGAGTAACTCAAAAACCCTGTTAATAACAGACTaaa                             <  1:2540148/150‑1 (MQ=255)

                                                                                                                          ccaccaGTAAGTGAGCCGATGAAATAACCAAACATTAATGCCGAGGTAAAGGCGGCATTGAGGAAATTATTTGACCATCCATTGCTTACCAGCTTTGCTAAGACGACATTACCGGAGTAACTCAAAAACCCTGTTAATAACAGACTAAAg                            >  1:1002036/1‑150 (MQ=255)

                                                                                                                           caccaGTAAGTGAGCCGATGAAATAACCAAACATTAATGCCGAGGTAAAGGCGGCATTGAGGAAATTATTTGACCATCCATTGCTTACCAGCTTTGCTAAGACGACATTACCGGAGTAACTCAAAAACCCTGTTAATAACAGACTAAAGc                           >  2:842503/1‑150 (MQ=255)

                                                                                                                              caGTAAGTGAGCCGATGAAATAACCAAACATTAATGCCGAGGTAAAGGCGGCATTGAGGAAATTATTTGACCATCCATTGCTTACCAGCTTTGCTAAGACGACATTACCGGAGTAACTCAAAAACCCTGTTAATAACAGACTAAAGCTta                        >  1:689316/1‑150 (MQ=255)

                                                                                                                                gTAAGTGAGCCGATGAAATAACCAAACATTAATGCCGAGGTAAAGGCGGCATTGAGGAAATTATTTGACCATCCATTGCTTACCAGCTTTGCTAAGACGACATTACCGGAGTAACTCAAAAACCCTGTTAATAACAGACTAAAGCTtata                      <  2:2919063/150‑1 (MQ=255)

                                                                                                                                   aGTGAGCCGATGAAATAACCAAACATTAATGCCGAGGTAAAGGCGGCATTGAGGAAATTATTTGACCATCCATTGCTTACCAGCTTTGCTAAGACGACATTACCGGAGTACCTCAAAAACCCTGTTAATAACAGACTCAAGCTTatcata                   >  2:504719/1‑150 (MQ=255)

                                                                                                                                   aGTGAGCCGATGAAATAACCAAACATTAATGCCGAGGTAAAGGCGGCATTGAGGAAATTATTTGACCATCCATTGCTTACCAGCTTTGCTAAGACGACATTACCGGAGTAACTCAAAAACCCTGTTAATAACAGACTAAAGCTTataata                   <  2:384569/150‑1 (MQ=255)

                                                                                                                                      gAGCCGATGAAATAACCAAACATTAATGCCGAGGTAAAGGCGGCATTGAGGAAATTATTTGACCATCCATTGCTTACCAGCTTTGCTAAGACGACATTACCGGAGTAACTCAAAAACCCTGTTAATAACAGACTAAAGCTTATAATACCa                >  1:4318657/1‑150 (MQ=255)

                                                                                                                                        gCCGATGAAATAACCAAACATTAATGCCGAGGTAAAGGCGGCATTGAGGAAATTATTTGACCATCCATTGCTTACCAGCTTTGCTAAGACGACATTACCGGAGTAACTCAAAAACCCTGTTAATAACAGACTAAAGCTTATAATACCaaa              <  1:1534424/150‑1 (MQ=255)

                                                                                                                                        gCCGATGAAATAACCAAACATTAATGCCGAGGTAAAGGCGGCATTGAGGAAATTATTTGACCATCCATTGCTTACCAGCTTTGCTAAGACGACATTACCGGAGTAACTCAAAAACCCTGTTAATAACAGACTAAAGCTTATAATACCaaa              <  2:1918382/150‑1 (MQ=255)

                                                                                                                                          cGATGAAATAACCAAACATTAATGCCGAGGTAAAGGCGGCATTGAGGAAATTATTTGACCATCCATTGCTTACCAGCTTTGCTAAGACGACATTACCGGAGTAACTCAAAAACCCTGTTAATAACAGACTAAAGCTTATAATACCAAata            <  1:1498375/150‑1 (MQ=255)

                                                                                                                                           gATGAAATAACCAAACATTAATGCCGAGGTAAAGGCGGCATTGAGGAAATTATTTGACCATCCATTGCTTACCAGCTTTGCTAAGACGACATTACCGGAGTAACTCAAAAACCCTGTTAATAACAGACTAAAGCTTATAATACCAAatat           <  1:4657083/150‑1 (MQ=255)

                                                                                                                                           gATGAAATAACCAAACATTAATGCCGAGGTAAAGGCGGCATTGAGGAAATTATTTGACCATCCATTGCTTACCAGCTTTGCTAAGACGACATTACCGGAGTAACTCAAAAACCCTGTTAATAACAGACTAAAGCTTATAATAACAAatat           <  2:3118250/150‑1 (MQ=255)

                                                                                                                                            aTGAAATAACCAAACATTAATGCCGAGGTAAAGGCGGCATTGAGGAAATTATTTGACCATCCATTGCTTACCAGCTTTGCTAAGACGACATTACCGGAGTAACTCAAAAACCCTGTTAATAACAGACTAAAGCTTATAATACCAAAtata          >  2:4663040/1‑150 (MQ=255)

                                                                                                                                            aTGAAATAACCAAACATTAATGCCGAGGTAAAGGCGGCATTGAGGAAATTATTTGACCATCCATTGCTTACCAGCTTTGCTAAGACGACATTACCGGAGTAACTCAAAAACCCTGTTAATAACAGACTAAAGCTTATAATACCAAAtata          <  2:1509907/150‑1 (MQ=255)

                                                                                                                                             tGAAATAACCAAACATTAATGCCGAGGTAAAGGCGGCATTGAGGAAATTATTTGACCATCCATTGCTTACCAGCTTTGCTAAGACGACATTACCGGAGTAACTCAAAAACCCTGTTAATAACAGACTAAAGCTTATAATACCAAATATAc         >  1:3655658/1‑150 (MQ=255)

                                                                                                                                             tGAAATAACCAAACATTAATGCCGAGGTAAAGGCGGCATTGAGGAAATTATTTGACCATCCATTGCTTACCAGCTTTGCTAAGACGACATTACCGGAGTAACTCAAAAACCCTGTTAATAACAGACTAAAGCTTATAATACCAAATATAc         >  1:4901058/1‑150 (MQ=255)

                                                                                                                                             tGAAATAACCAAACATTAATGCCGAGGTAAAGGCGGCATTGAGGAAATTATTTGACCATCCATTGCTTACCAGCTTTGCTAAGACGACATTACCGGAGTAACTCAAAAACCCTGTTAATAACAGACTAAAGCTTATAATACCAAATATAc         <  2:1552487/150‑1 (MQ=255)

                                                                                                                                                 aTAACCAAACATTAATGCCGAGGTAAAGGCGGCATTGAGGAAATTATTTGACCATCCATTGCTTACCAGCTTTGCTAAGACGACATTACCGGAGTAACTCAAAAACCCTGTTAATAACAGACTAAAGCTTATAATACCAAATATACGATa     >  2:1028333/1‑150 (MQ=255)

                                                                                                                                                    aCCAAACATTAATGCCGAGGTAAAGGCGGCATTGAGGAAATTATTTGACCATCCATTGCTTACCAGCTTTGCTAAGACGACATTACCGGAGTAACTCAAAAACCCTGTTAATAACAGACTAAAGCTTATAATACCAAATATACGATAATg  <  2:2996257/150‑1 (MQ=255)

GGAAGCGAAAGAAGATGAGCCAGTACATATCAGGGACAAAAGCGGCCCCTGTTGCAGCAATACCGACGATGAGAAGATTTATGCGAAACGCCCTGCGCCGCCCAAAGTAGTCACCAATAAACCCACCAGTAAGTGAGCCGATGAAATAACCAAACATTAATGCCGAGGTAAAGGCGGCATTGAGGAAATTATTTGACCATCCATTGCTTACCAGCTTTGCTAAGACGACATTACCGGAGTAACTCAAAAACCCTGTTAATAACAGACTAAAGCTTATAATACCAAATATACGATAATG  >  CP009273/1847882‑1848179

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`