Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,850,797 C→A 11.6% R130L (CGG→CTG)  ydjH ← putative kinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,850,7970CA11.6% 1031.9 / 73.0 300R130L (CGG→CTG) ydjHputative kinase
Reads supporting (aligned to +/- strand):  ref base C (145/119);  new base A (14/22);  total (159/141)
Fisher's exact test for biased strand distribution p-value = 7.73e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 309 total aligned reads displayed.

GTTTCATTCAACCGCGGTTTGATCATATCGGCACAGATAATCATCTGCCGGGCTTTAGCTTGCGTAAAAATTTCTGTTAGCGCTTTACCATCCAATAGTGGACTGTTGAAAATACTGGCCAGTGATAATAATTTCGCCTGAGAAAACCGCGCAAAATCAACATCGTCAATATTCAATTTCCACAGACTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTCACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAATATTC  >  CP009273/1850650‑1850946
                                                                                                                                                   |                                                                                                                                                     
 tttCATTCAACCGCGGTTTGATCATATCGGCACAGATAATCATCTGCCGGGCTTTAGCTTGCGTAAAAATTTCTGTTAGCGCTTTACCATCCAATAGTGGACTGTTGAAAATACTGGCCAGTGATAATAATTTCGCCTGAGAAAACcgcg                                                                                                                                                    <  1:500103/150‑1 (MQ=255)
  ttcattcaACCGCGGTTTGATCATATCGGCACAGATAATCATCTGCCGGGCTTTAGCTTGCGTAAAAATTTCTGTTAGCGCTTTACCATCCAATAGTGGACTGTTGAAAATACTGGCCAGTGATAATAATTTCGCCTGAGAAAACCgcgc                                                                                                                                                   >  2:5092670/1‑150 (MQ=255)
   tcattcaACCGCGGTTTGATCATATCGGCACAGATAATCATCTGCCGGGCTTTAGCTTGCGTAAAAATTTCTGTTAGCGCTTTACCATCCAATAGTGGACTGTTGAAAATACTGGCCAGTGATAATAATTTCGCCTGAGAAAACCGCGCa                                                                                                                                                  >  1:2668873/1‑150 (MQ=255)
   tcagtcaACCGCGGTTTGATCATATCGGCACAGATAATCATCTGCCGGGCTTTAGCGTGCGTAAAAATGTCTGTTAGCGCTTTACCATCCAAGAGTGGACTGTTGAAAATACGGGCCAGTGATAATAATTTCGCCTGAGAAAACCGCGCa                                                                                                                                                  <  1:3991254/150‑1 (MQ=255)
     attcaACCGCGGTTTGATCATATCGGCACAGATAATCATCTGCCGGGCTTTAGCTTGAGTAAAAATTTCTGTTAGAGCTTTACCATCCAATAGTGGACTGTTGAAAATACTGGCCAGTGATAATAATTTCGCCTGAGAAAACCGCGCaaa                                                                                                                                                >  1:559021/1‑150 (MQ=255)
            cgcgGTTTGATCATATCGGCACAGATAATCATCTGCCGGGCTTTAGCTTGCGTAAAAATTTCTGTTAGCGCTTTACCATCCAATAGTGGACTGTTGAAAATACTGGCCAGTGATAATAATTTCGCCTGAGAAAACAGCGCACAATCAACa                                                                                                                                         >  2:4628066/1‑150 (MQ=255)
              cgGTTTGATCATATCGGCACAGATAATCATCTGCCGGGCTTTAGCTTGCGTAAAAATTTCTGTTAGCGCTTTACCATCCAATAGTGGACTGTTGAAAATACTGGCCAGTGATAATAATTTCGCCTGAGAAAACCGCGCAAAATCAACATc                                                                                                                                       >  1:3759823/1‑150 (MQ=255)
              cgGTTTGATCATATCGGCACAGATAATCATCTGCCGGGCTTTAGCTTGCGTAAAAATTTCTGTTAGCGCTTTACCATCCAATAGTGGACTGTTGAAAATACTGGCCAGTGATAATAATTTCGCCTGAGAAAACAGCGCAAAATCAACATc                                                                                                                                       >  2:4270053/1‑150 (MQ=255)
               ggTTTGATCATATCGGCACAGATAATCATCTGCCGGGCTTTAGCTTGCGTAAAAATTTCTGTTAGCGCTTTACCATCCAATAGTGGACTGTTGAAAATACTGGCCAGTGATAATAATTTCGCCTGAGAAAACCGCGCAAAATCAACATCg                                                                                                                                      >  2:302281/1‑150 (MQ=255)
               ggTTTGATCATATCGGCACAGATAATCATCTGCCGGGCTTTAGCTTGCGTAAAAATTTCTGTTAGCGCTTTACCATCCAATAGTGGACTGTTGAAAATACTGGCCAGTGATAATAATTTCGCCTGAGAAAACCGCGCAAAATCAACATCg                                                                                                                                      <  2:1728313/150‑1 (MQ=255)
               ggTTTGATCATATCGGCACAGATAATCATCTGCCGGGCTTTAGCTTGCGTAAAAATTTCTGTTAGCGCTTTACCATCCAATAGTGGACTGTTGAAAATACTGGCCAGTGATAATAATTTCGCCTGAGAAAACCGCGCAAAATCAACATCg                                                                                                                                      >  1:2393180/1‑150 (MQ=255)
                gTTTGATCATATCGGCACAGATAATCATCTGCCGGGCTTTAGCTTGCGTAAAAATTTCTGTTAGCGCTTTACCATCCAATAGTGGACTGTTGAAAATACTGGCCAGTGATAATAATTTCGCCTGAGAAAACCGCGCAAAATCAACATCGt                                                                                                                                     <  2:481486/150‑1 (MQ=255)
                 tttGATCATATCGGCACAGATAATCATCTGCCGGGCTTTAGCTTGCGTAAAAATTTCTGTTAGCGCTTTACCATCCAATAGTGGACTGTTGAAAATACTGGCCAGTGATAATAATTTCGCCTGAGAAAACCGCGCAAAATCAACATCGTc                                                                                                                                    >  2:857429/1‑150 (MQ=255)
                  ttGATCATATCGGCACAGATAATCATCTGCCGGGCTTTAGCTTGCGTAAAAATTTCTGTTAGCGCTTTACCATCCAATAGTGGACTGTTGAAAATACTGGCCAGTGATAATAATTTCGCCTGAGAAAACCGCGCAAAATCAACATCGTCa                                                                                                                                   >  1:4047685/1‑150 (MQ=255)
                  ttGATCATATCGGCACAGATAATCATCTGCCGGGCTTTAGCTTGCGTAAAAATTTCTGTTAGCGCTTTACCATCCAATAGTGGACTGTTGAAAATACTGGCCAGTGATAATAATTTCGCCTGAGAAAACCGCGCAAAATCAACATCGTCa                                                                                                                                   >  1:2198849/1‑150 (MQ=255)
                  ttGATCATATCGGCACAGATAATCATCTGCCGGGCTTTAGCTTGCGTAAAAATTTCTGTTAGCGCTTTACCATCCAATAGTGGACTGTTGAAAATACTGGCCAGTGATAATAATTTCGCCTGAGAAAACCGCGCAAAATCAACATCGTCa                                                                                                                                   >  2:651791/1‑150 (MQ=255)
                    gATCATATCGGCACAGATAATCATCTGCCGGGCTTTAGCTTGCGTAAAAATTTCTGTTAGCGCTTTACCATCCAATAGTGGACTGTTGAAAATACTGGCCAGTGATAATAATTTCGCCTGAGAAAACCGCGCAAAATCAACATCGTCAat                                                                                                                                 >  1:160046/1‑150 (MQ=255)
                    gATCATATCGGCACAGATAATCATCTGCCGGGCTTTAGCTTGCGTAAAAATTTCTGTTAGCGCTTTACCATCCAATAGTGGACTGTTGAAAATACTGGCCAGTGATAATAATTTCGCCTGAGAAAACCGCGCAAAATCAACATCGTCAat                                                                                                                                 >  1:1175856/1‑150 (MQ=255)
                    gATCATATCGGCACAGATAATCATCTGCCGGGCTTTAGCTTGCGTAAAAATTTCTGTTAGCGCTTTACCATCCAATAGTGGACTGTTGAAAATACTGGCCAGTGATAATAATTTCGCCTGAGAAAACCGCGCAAAATCAACATCGTCAat                                                                                                                                 <  1:1827861/150‑1 (MQ=255)
                    gATCATATCGGCACAGATAATCATCTGCCGGGCTTTAGCTTGCGTAAAAATTTCTGTTAGCGCTTTACCATCCAATAGTGGACTGTTGAAAATACTGGCCAGTGATAATAATTTCGCCTGAGAAAACAGCGCAAAATCAACATCGTCAat                                                                                                                                 >  1:4862913/1‑150 (MQ=255)
                      tCATATCGGCACAGATAATCATCTGCCGGGCTTTAGCTTGCGTAAAAATTTCTGTTAGCGCTTTACCATCCAATAGTGGACTGTTGAAAATACTGGCCAGTGATAATAATTTCGCCTGAGAAAACCGCGCAAAATCAACATCGTCAatat                                                                                                                               >  1:4111722/1‑150 (MQ=255)
                       cATATCGGCACAGATAATCATCTGCCGGGCTTTAGCTTGCGTAAAAATTTCTGTTAGCGCTTTACCATCCAATAGTGGACTGTTGAAAATACTGGCCAGTGATAATAATTTCGCCTGAGAAAACCGCGCAAAATCAACATCGTCAATAtt                                                                                                                              <  2:5137572/150‑1 (MQ=255)
                         tatCGGCACAGATAATCATCTGCCGGGCTTTAGCTTGCGTAAAAATTTCTGTTAGCGCTTTACCATCCAATAGTGGACTGTTGAAAATACTGGCCAGTGATAATAATTTCGCCTGAGAAAACCGCGCAAAATCAACATCGTCAATATTCa                                                                                                                            >  2:1346439/1‑150 (MQ=255)
                         tatCGGCACAGATAATCATCTGCCGGGCTTTAGCTTGCGTAAAAATTTCTGTTAGCGCTTTACCATCCAATAGTGGACTGTTGAAAATACTGGCCAGTGATAATAATTTCGCCTGAGAAAACAGCGCAAAATCAACATCGTCAATATTCa                                                                                                                            <  1:4218368/150‑1 (MQ=255)
                          atCGGCACAGATAATCATCTGCCGGGCTTTAGCTTGCGTAAAAATTTCTGTTAGCGCTTTACCATCCAATAGTGGACTGTTGAAAATACTGGCCAGTGATAATAATTTCGCCTGAGAAAACCGCGCAAAATCAACATCGTCAATATTCaa                                                                                                                           <  1:2834735/150‑1 (MQ=255)
                           tCGGCACAGATAATCATCTGCCGGGCTTTAGCTTGCGGAAACATTTCGGGTAGCGCTTTACCATCCAATAGTGGACTGTTGAAAATACTGGCCAGTGATAATAATTTCGCCTGAGAAAACCGCGCAAAATCAACATCGGCAATATTCAAt                                                                                                                          >  1:2879707/1‑150 (MQ=255)
                             ggCACAGATAATCATCTGCCGGGCTTTAGCTTGCGTAAAAATTTCTGTTAGCGCTTTACCATCCATTAGTGGACTGTTGATAATACTGGCGAGTGATAATATTTTCGCCTGAGAAAACCGCGCAAAATCAACATCGTCAATATTCAAttt                                                                                                                        <  2:2909492/150‑1 (MQ=255)
                               cacaTATAATCATCTGCCGGGCTTTAGCTTGCGTAAAAATTTATGTTAGCGCTTTACCATCCAATAGTGGACTGTTGAAAATACTGGCCAGTGATAATAATTTCGCCTGAGAAAACCGCGCAAAATCAACATCGTCAATATTCAATTTcc                                                                                                                      <  2:4954425/150‑1 (MQ=255)
                                acaGATAATCATCTGCCGGGCTTTAGCTTGCGTAAAAATTTCTGTTAGCGCTTTACCATCCAATAGTGGACTGTTGAAAATACTGGCCAGTGATAATAATTTCGCCTGAGAAAACCGCGCAAAATCAACATCGTCAATATTCAATTTCca                                                                                                                     <  2:4001026/150‑1 (MQ=255)
                                 caGATAATCATCTGCCGGGCTTTAGCTTGCGTAAAAATTTCTGTTAGCGCTTTACCATCCAATAGTGGACTGTTGAAAATACTGGCCAGTGATAATAATTTCGCCTGAGAAAACCGCGCAAAATCAACATCGTCAATATTCAATTTCcac                                                                                                                    <  1:4576011/150‑1 (MQ=255)
                                     tAATCATCTGCCGGGCTTTAGCTTGCGTAAAAATTTCTGTTAGCGCTTTACCATCCAATAGTGGACTGTTGAAAATACTGGCCAGTGATAATAATTTCGCCTGAGAAAACAGCGCAAAATCAACATCGTCAATATTCAATTTCCACAGAc                                                                                                                <  1:966823/150‑1 (MQ=255)
                                        tcatcTGCCGGGCTTTAGCTTGCGTAAAAATTTCTGTTAGCGCTTTACCATCCAATAGTGGACTGTTGAAAATACTGGCCAGTGATAATAATTTCGCCTGAGAAAACCGCGCAAAATCAACATCGTCAATATTCAATTTCCACAGACTGc                                                                                                             >  2:3591186/1‑150 (MQ=255)
                                         catcTGCCGGGCTTTAGCTTGCGTAAAAATTTCTGTTAGCGCTTTACCATCCAATAGTGGACTGTTGAAAATACTGGCCAGTGATAATAATTTCGCCTGAGAAAACCGCGCAAAATCAACATCGTCAATATTCAATTTCCACAGACTGcc                                                                                                            >  2:2077849/1‑150 (MQ=255)
                                            cTGCCGGGCTTTAGCTTGCGTAAAAATTTCTGTTAGCGCTTTACCATCCAATAGTGGACTGTTGAAAATACTGGCCAGTGATAATAATTTCGCCTGAGAAAACCGCGCAAAATCAACATCGTCAATATTCAATTTCCACAGACTGCCAtt                                                                                                         <  1:1003599/150‑1 (MQ=255)
                                             tGCCGGTCTTTAGATTGCTTAAAATTTTCTGTTAGCGCTTTACCATCCAATAGTGGACTGTTGAAAATACTGGCCAGTGATAATAATTTCGCCTGAGAAAACCGCGCAAAATCAACATCGTCAATATTCAATTTCCACAGACTGCCATTa                                                                                                        <  1:3703070/150‑1 (MQ=255)
                                             tGCCGGGCTTTAGCTTGCGTAAAAATTTCTGTTAGCGCTTTACCATCCAATAGTGGACTGTTGAAAATACTGGCCAGTGATAATAATTTCGCCTGAGAAAACCGCGCAAAATCAACATCGTCAATATTCAATTTCCACAGACTGCCATTa                                                                                                        >  1:2548783/1‑150 (MQ=255)
                                             tGCCGGGCTTTAGCTTGCGTAAAAATTTCTGTTAGCGCTTTACCATCCAATAGTGGACTGTTGAAAATACTGGCCAGTGATAATAATTTCGCCTGAGAAAACCGCGCAAAATCAACATCGTCAATATTCAATTTCCACAGACTGCCATTa                                                                                                        >  2:2594798/1‑150 (MQ=255)
                                               ccGGGCTTTAGCTTGCGTAAAAATTTCTGTTAGCGCTTTACCATCCAATAGTGGACTGTTGAAAATACTGGCCAGTGATAATAATTTCGCCTGAGAAAACCGCGCAAAATCAACATCGTCAATATTCAATTTCCACAGACTGCCATTACg                                                                                                      >  2:2615021/1‑150 (MQ=255)
                                                cGGGCTTTAGCTTGCGTAAAAATTTCTGTTAGCGCTTTACCATCCAATAGTGGACTGTTGAAAATACTGGCCAGTGATAATAATTTCGCCTGAGAAAACCGCGCAAAATCAACATCGTCAATATTCAATTTCCACAGACTGCCATTACgg                                                                                                     >  1:3352793/1‑150 (MQ=255)
                                                cGGGCTTTAGCTTGCGTAAAAATTTCTGTTAGCGCTTTACCATCCAATAGTGGACTGTTGAAAATACTGGCCAGTGATAATAATTTCGCCTGAGAAAACCGCGCAAAATCAACATCGTCAATATTCAATTTCCACAGACTGCCATTACgg                                                                                                     <  2:294199/150‑1 (MQ=255)
                                                 gggCTTTAGCTTGCGTAAAAATTTCTGTTAGCGCTTTACCATCCAATAGTGGACTGTTGAAAATACTGGCCAGTGATAATAATTTCGCCTGAGAAAACAGCGCAAAATCAACATCGTCAATATTCAATTTCCACAGACTGCCATTACGGt                                                                                                    >  1:4283034/1‑150 (MQ=255)
                                                       tAGCTTGCGTAAAAATTTCTGTTAGCGCTTGACCATCCAATAGTGGACTGTTGAAAATACTGGCCAGTGATAAGAATTTCGCCTGAGAAAACCGCGCAAAATCAACATCGTCAATATTCAATTTCCACAGACTGCCAGTACGGTTTGTGa                                                                                              <  2:3675561/150‑1 (MQ=255)
                                                          cTTGCGTAAAAATTTCTGTTAGCGCTTTACCATCCAATAGTGGACTGTTGAAAATACTGGCCAGTGATAATAATTTCGCCTGAGAAAACCGCGCAAAATCAACATCGTCAATATTCAATTTCCACAGACTGCCATTACGGTTTGTGACaa                                                                                           >  1:1698275/1‑150 (MQ=255)
                                                              cGTAAAAATTTCTGTTAGCGCTTTACCATCCAATAGTGGACTGTTGAAAATACTGGCCAGTGATAATAATTTCGCCTGAGAAAACCGCGCAAAATCAACATCGTCAATATTCAATTTCCACAGACTGCCATTACGGTTTGTGACAAACGt                                                                                       <  1:844808/150‑1 (MQ=255)
                                                                 aaaaaTTTCTGTTAGCGCTTTACCATCCAATAGTGGACTGTTGAAAATACTGGCCAGTGATAATAATTTCGCCTGAGAAAACCGCGCAAAATCAACATCGTCAATATTCAATTTCCACAGACTGCCATTACGGTTTGTGACAAACGTCCg                                                                                    >  2:4383525/1‑150 (MQ=255)
                                                                 aaaaaTTTCTGTTAGCGCTTTACCATCCAATAGTGGACTGTTGAAAATACTGGCCAGTGATAATAATTTCGCCTGAGAAAACCGCGCAAAATCAACATCGTCAATATTCAATTTCCACAGACTGCCATTACGGTTTGTGACAAACGTCCg                                                                                    >  2:644583/1‑150 (MQ=255)
                                                                  aaaaTTTCTGTTAGCGCTTTACCATCCAATAGTGGACTGTTGAAAATACTGGCCAGTGATAATAATTTCGCCTGAGAAAACCGCGCAAAATCAACATCGTCAATATTCAATTTCCACAGACTGCCATTACGGTTTGTGACAAACGTCCGc                                                                                   <  2:1011485/150‑1 (MQ=255)
                                                                  aaaaTTTCTGTTAGCGCTTTACCATCCAATAGTGGACTGTTGAAAATACTGGCCAGTGATAATAATTTCGCCTGAGAAAACCGCGCAAAATCAACATCGTCAATATTCAATTTCCACAGACTGCCATTACGGTTTGTGACAAACGTCCGc                                                                                   <  2:4119905/150‑1 (MQ=255)
                                                                    aaTTTCTGTTAGCGCTTTACCATCCAATAGTGGACTGTTGAAAATACTGGCCAGTGATAATAATTTCGCCTGAGAAAACCGCGCAAAATCAACATCGTCAATATTCAATTTCCACAGACTGCCATTACGGTTTGTGACAAACGTCCGCTc                                                                                 <  2:4474732/150‑1 (MQ=255)
                                                                       ttCTGTTAGCGCTTTACCATCCAATAGTGGACTGTTGAAAATACTGGCCAGTGATAATAATTTCGCCTGAGAAAACCGCGCAAAATCAACATCGTCAATATTCAATTTCCACAGACTGCCATTACGGTTTGTGACAAACGTCCGCTCGcc                                                                              >  2:4924493/1‑150 (MQ=255)
                                                                        tCTGTTAGCGCTTTACCATCCAATAGTGGACTGTTGAAAATACTGGCCAGTGATAATAATTTCGCCTGAGAAAACCGCGCAAAATCAACATCGTCAATATTCAATTTCCACAGACTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCa                                                                             <  2:489762/150‑1 (MQ=255)
                                                                        tCTGTTAGCGCTTTACCATCCAATAGTGGACTGTTGAAAATACTGGCCAGTGATAATAATTTCGCCTGAGAAAACCGCGCAAAATCAACATCGTCAATATTCAATTTCCACAGACTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCa                                                                             <  2:2328721/150‑1 (MQ=255)
                                                                          tGTTAGCGCTTTACCATCCAATAGTGGACTGTTGAAAATACTGGCCAGTGATAATAATTTCGCCTGAGAAAACCGCGCAAAATCAACATCGTCAATATTCAATTTCCACAGACTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCAtc                                                                           <  1:1325787/150‑1 (MQ=255)
                                                                          tGTTAGCGCTTTACCATCCAATAGTGGACTGTTGAAAATACTGGCCAGTGATAATAATTTCGCCTGAGAAAACAGCGCAAAATCAACATCGTCAATATTCAATTTCCACAGACTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCAtc                                                                           <  2:1798086/150‑1 (MQ=255)
                                                                           gTTAGCGCTTTACCATCCAATAGTGGACTGTTGAAAATACTGGCCAGTGATAATAAATTCGCCTGAGAAAACCGCGCAAAATCAACATCGTCAATATTCAATTTCCACAGACTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCAtcc                                                                          >  1:5136681/1‑150 (MQ=255)
                                                                             tAGCGCTTTACCATCCAATAGTGGACTGTTGAAAATACTGGCCAGTGATAATAATTTCGCCTGAGAAAACCGCGCAAAATCAACATCGTCAATATTCAATTTCCACAGACTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCAtcctc                                                                        >  2:848792/1‑150 (MQ=255)
                                                                             tAGCGCTTTACCATCCAATAGTGGACTGTTGAAAATACTGGCCAGTGATAATAATTTCGCCTGAGAAAACCGCGCAAAATCAACATCGTCAATATTCAATTTCCACAGACTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCAtcctc                                                                        <  2:878649/150‑1 (MQ=255)
                                                                              aGCGCTTTACCATCCAATAGTGGACTGTTGAAAATACTGGCCAGTGATAATAATTTCGCCTGAGAAAACAGCGCAAAATCAACATCGTCAATATTCAATTTCCACAGACTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCAtcctcc                                                                       <  1:3238353/150‑1 (MQ=255)
                                                                               gcgcTTTACCATCCAATAGTGGACTGTTGAAAATACTGGCCAGTGATAATAATTTCGCCTGAGAAAACCGCGCAAAATCAACATCGTCAATATTCAATTTCCACAGACTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCg                                                                      <  2:3124847/150‑1 (MQ=255)
                                                                               gcgcTTTACCATCCAATAGTGGACTGTTGAAAATACTGGCCAGTGATAATAATTTCGCCTGAGAAAACCGCGCAAAATCAACATCGTCAATATTCAATTTCCACAGACTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCg                                                                      <  1:648169/150‑1 (MQ=255)
                                                                               gcgcTTTACCATCCAATAGTGGACTGTTGAAAATACTGGCCAGTGATAATAATTTCGCCTGAGAAAACCGCGCAAAATCAACATCGTCAATATTCAATTTCCACAGACTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCg                                                                      <  2:76053/150‑1 (MQ=255)
                                                                               gcgcTTTACCATCCAATAGTGGACTGTTGAAAATACTGGCCAGTGATAATAATTTCGCCTGAGAAAACCGCGCAAAATCAACATCGTCAATATTCAATTTCCACAGACTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCg                                                                      >  1:1379929/1‑150 (MQ=255)
                                                                               gcgcTTTACCATCCAATAGTGGACTGTTGAAAATACTGGCCAGTGATAATAATTTCGCCTGAGAAAACCGCGCAAAATCAACATCGTCAATATTCAATTTCCACAGACTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCg                                                                      <  2:535806/150‑1 (MQ=255)
                                                                                   tttACCATCCAATAGTGGACTGTTGAAAATACTGGCCAGTGATAATAATTTCGCCTGAGAAAACCGCGCAAAATCAACATCGTCAATATTCAATTTCCACAGACTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTAAc                                                                  >  1:2738178/1‑150 (MQ=255)
                                                                                         aTCCAATAGTGGACTGTTGAAAATACTGGCCAGTGATAATAATTTCGCCTGAGAAAACCGCGCAAAATCAACATCGTCAATATTCAATTTCCACAGACTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTAACCAGTcc                                                            >  1:3507481/1‑150 (MQ=255)
                                                                                         aTCCAATAGTGGACTGTTGAAAATACTGGCCAGTGATAATAATTTCGCCTGAGAAAACCGCGCAAAATCAACATCGTCAATATTCAATTTCCACAGACTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTAACCAGTcc                                                            <  1:774478/150‑1 (MQ=255)
                                                                                          tCCAATAGTGGACTGTTGAAAATACTGGCCAGTGATAATAATTTCGCCTGAGAAAACCGCGCAAAATCAACATCGTCAATATTCAATTTCCACAGACTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTCACCAGTCCa                                                           <  2:2408595/150‑1 (MQ=255)
                                                                                          tCCAATAGTGGACTGTTGAAAATACTGGCCAGTGATAATAATTTCGCCTGAGAAAACCGCGCAAAATCAACATCGTCAATATTCAATTTCCACAGACTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTCACCAGTCCa                                                           <  2:3901694/150‑1 (MQ=255)
                                                                                           ccAATAGTGGACTGTTGAAAATACTGGCCAGTGATAATAATTTCGCCTGAGAAAACAGCGCAAAATCAACATCGTCAATATTCAATTTCCACAGACTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTAACCAGTCCaa                                                          <  1:3090896/150‑1 (MQ=255)
                                                                                             aaTAGTGGACTGTTGAAAATACTGGCCAGTGATAATAATTTCGCCTGAGAAAACCGCGCAAAATCAACATCGTCAATATTCAATTTCCACAGACTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTCACCAGTCCAACg                                                        >  2:2237918/1‑150 (MQ=255)
                                                                                             aaTAGTGGACTGTTGAAAATACTGGCCAGTGATAATAATTTCGCCTGAGAAAACCGCGCAAAATCAACATCGTCAATATTCAATTTCCACAGACTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTCACCAGTCCAACg                                                        <  1:1778819/150‑1 (MQ=255)
                                                                                                aGTGGACTGTTGAAAATACTGGCCAGTGATAATAATTTCGCCTGAGAAAACCGCGCAAAATCAACATCGTCAATATTCAATTTCCACAGACTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTCACCAGTCCAACGTTa                                                     >  2:3354195/1‑150 (MQ=255)
                                                                                                  tGGACTGTTGAAAATACTGGCCAGTGATAATAATTTCGCCTGAGAAAACCGCGCAAAATCAACATCGTCAATATTCAATTTCCACAGACTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTCACCAGTCCAACGTTAAt                                                   <  1:2262172/150‑1 (MQ=255)
                                                                                                     aCTGTTGAAAATACTGGCCAGTGATAATAATTTCGCCTGAGAAAACCGCGCAAAATCAACATCGTCAATATTCAATTTCCACAGACTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTAACCAGTCCAACGTTAATaga                                                >  1:373980/1‑150 (MQ=255)
                                                                                                      cTGTTGAAAATACTGGCCAGTGATAATAATTTCGCCTGAGAAAACCGCGCAAAATCAACATCGTCAATATTCAATTTCCACAGACTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTCACCAGTCCAACGTTAATagag                                               >  2:4048299/1‑150 (MQ=255)
                                                                                                      cTGTTGAAAATACTGGCCAGTGATAATAATTTCGCCTGAGAAAACCGCGCAAAATCAACATCGTCAATATTCAATTTCCACAGACTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTAACCAGTCCAACGTTAATagag                                               >  1:1744970/1‑150 (MQ=255)
                                                                                                      cTGTTGAAAATACTGGCCAGTGATAATAATTTCGCCTGAGAAAACAGCGCAAAATCAACATCGTCAATATTCAATTTCCACAGACTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTAACCAGTCCAACGTTAATagag                                               <  2:1421915/150‑1 (MQ=255)
                                                                                                        gTTGAAAATACTGGCCAGTGATAATAATTTCGCCTGAGAAAACCGCGCAAAATCAACATCGTCAATATTCAATTTCCACAGACTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTCACCAGTCCAACGTTAATAGAGGt                                             <  1:1978728/150‑1 (MQ=255)
                                                                                                            aaaaTACTGGCCAGTGATAATAATTTCGCCTGAGAAAACCGCGCAAAATCAACATCGTCAATATTCAATTTCCACAGACTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTCACCAGTCCAACGTTAATAGAGGTATCt                                         >  2:717696/1‑150 (MQ=255)
                                                                                                            aaaaTACTGGCCAGTGATAATAATTTCGCCTGAGAAAACAGCGCAAAATCAACATCGTCAATATTCAATTTCCACAGACTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTAACCAGTCCAACGTTAATAGAGGTATCt                                         >  1:2347680/1‑150 (MQ=255)
                                                                                                                tACTGGCCAGTGATAATAATTTCGCCTGAGAAAACCGCGCAAAATCAACATCGTCAATATTCAATTTCCACAGACTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTCACCAGTCCAACGTTAATAGAGGTATCTATGc                                     >  2:1609542/1‑150 (MQ=255)
                                                                                                                  cTGGCCAGTGATAATAATTTCGCCTGAGAAAACCGCGCAAAATCAACATCGTCAATATTCAATTTCCACAGACTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTCACCAGTCCAACGTTAATAGAGGTATCTATGCTg                                   <  1:1148480/150‑1 (MQ=255)
                                                                                                                   tGGCCAGTGATAATAATTTCGCCTGAGAAAACCGCGCAAAATCAACATCGTCAATATTCAATTTCCACAGACTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTCACCAGTCCAACGTTAATAGAGGTATCTATGCTGa                                  >  2:3284658/1‑150 (MQ=255)
                                                                                                                     gCCAGTGATAATAATTTCGCCTGAGAAAACCGCGCAAAATCAACATCGTCAATATTCAAGTTCCACAGACTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTAACCAGTCCAACGTTAATAGAGGTATCTATGCTGACg                                <  2:5017229/150‑1 (MQ=255)
                                                                                                                      ccAGTGATAATAATTTCGCCTGAGAAAACCGCGCAAAATCAACATCGTCAATATTCAATTTCCACAGACTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTAACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGt                               >  2:1628967/1‑150 (MQ=255)
                                                                                                                      ccAGTGATAATAATTTCGCCTGAGAAAACCGCGCAAAATCAACATCGTCAATATTCAATTTCCACAGACTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTAACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGt                               >  1:4642799/1‑150 (MQ=255)
                                                                                                                       cAGTGATAATAATTTCGCCTGAGAAAACCGCGCAAAATCAACATCGTCAATATTCAATTTCCACAGACTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTCACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTc                              >  2:2487081/1‑150 (MQ=255)
                                                                                                                       cAGTGATAATAATTTCGCCTGAGAAAACCGCGCAAAATCAACATCGTCAATATTCAATTTCCACAGACTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTCACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTc                              <  1:20743/150‑1 (MQ=255)
                                                                                                                         gTGATAATAATTTCGCCTGAGAAAACAGCGCAAAATCAACATCGTCAATATTCAATTTCCACAGACTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTAACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCt                            >  1:3403625/1‑150 (MQ=255)
                                                                                                                           gATAATAATTTCGCCTGAGAAAACCGCGCAAAATCAACATCGTCAATATTCAATTTCCACAGACTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTCACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGc                          >  1:3548006/1‑150 (MQ=255)
                                                                                                                            ataataATTTCGCCTGAGAAAACCGCGCAAAATCAACATCGTCAATATTCAATTTCCACAGACTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTCACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCt                         >  1:4752242/1‑150 (MQ=255)
                                                                                                                                  atTTCGCCTGAGAAAACCGCGCAAAATCAACATCGTCAATATTCAATTTCCACAGACTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTCACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGc                   <  1:1507797/150‑1 (MQ=255)
                                                                                                                                   tttCGCCTGAGAAAACCGCGCAAAATCAACATCGTCAATATTCAATTTCCACAGACTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTCACCAGTCCAACGTTAATATAGTAATCTATGCTGACGACCTGATTCAGGCt                  >  2:2342949/1‑150 (MQ=255)
                                                                                                                                   tttCGCCTGAGAAAACCGCGCAAAATCAACATCGTCAATATTCAATGTCCACAGACTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTCACCAGTCCAACGTTAATATAGGTATCTATGCTGACGTCCTGCTTCAGGCt                  <  1:929647/150‑1 (MQ=255)
                                                                                                                                     tCGCCTGAGAAAACCGCGCAAAATCAACATCGTCAATATTCAATTTCCACAGACTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTCACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCttt                >  2:1074942/1‑150 (MQ=255)
                                                                                                                                          tGAGAAAACCGCGCAAAATCAACATCGTCAATATTCAATTTCCACAGACTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTCACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAata           >  2:1437762/1‑150 (MQ=255)
                                                                                                                                          tGAGAAAACAGCGCAAAATCAACATCGTCAATATTCAATTTCCACAGACTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTAACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAata           <  2:1595756/150‑1 (MQ=255)
                                                                                                                                           gagaAAACCGCGCAAAATCAACATCGTCAATATTCAATTTCCACAGACTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTCACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAatat          <  2:1122820/150‑1 (MQ=255)
                                                                                                                                           gagaAAACCGCGCAAAATCAACATCGTCAATATTCAATTTCCACAGACTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTCACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAatat          >  1:1576701/1‑150 (MQ=255)
                                                                                                                                                 aCCGCGCAAAATCAACATCGTCAATATTCAAGTTCCACAGACTGCCATTACGGTGTGTGACAAACGTCCGCTCGCCATCCTCCGTCACCAGTCCAACGGTAATAGAGGTATCTATGCTGACGTCCTGCTGCAGGCTTTGACGAGCAatat    >  2:2702576/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                     
GTTTCATTCAACCGCGGTTTGATCATATCGGCACAGATAATCATCTGCCGGGCTTTAGCTTGCGTAAAAATTTCTGTTAGCGCTTTACCATCCAATAGTGGACTGTTGAAAATACTGGCCAGTGATAATAATTTCGCCTGAGAAAACCGCGCAAAATCAACATCGTCAATATTCAATTTCCACAGACTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTCACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAATATTC  >  CP009273/1850650‑1850946

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑