BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	1,942,066	G→T	30.6%	H115N (CAT→AAT)	yebC ←	UPF0082 family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	1,942,066	0	G	T	30.6%	653.3 / 271.9	328	H115N (CAT→AAT)	yebC	UPF0082 family protein
Reads supporting (aligned to +/- strand): ref base G (108/119); new base T (60/41); total (168/160)
Fisher's exact test for biased strand distribution p-value = 5.57e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 329 total aligned reads displayed.

GTCACAACGTCTTCAGCACCTGCTTCCAGTGCTGCTTCCATGATGGTGTCTTCATCGCCTTTCTCGAAGGAGATCACGCCTTTTTTGCTGAACAGATAGGCAACGGAACCATCAGTACCGAGGTTACCGCCACATTTGCTAAATGCATGACGCACTTCAGCAACGGTACGGTTGCGGTTGTCAGACAGACATTCAATCATGATTGCCGTGCCGCCAGGACCGTAACCTTCGTAGATGATGGTTTCCATGTTTGCATCATCATCACCGCCCACACCACGTGCAATTGCGCGGTTCAGTG  >  CP009273/1941918‑1942215

gTCACAACGTCTTCAGCACCTGCTTCCAGTGCTGCTTCCATGATGGTGTCTTCATCGCCTTTCTCGAAGGAGATCACGCCTTTTTTGCTGAACAGATAGGCAACGGAACCATCAGTACCGAGGTTACCGCCACATTTGCTAAATGCATGa                                                                                                                                                      <  1:4081153/150‑1 (MQ=255)

 tCACAACGTCTTCAGCACCTGCTTCCAGTGCTGCTTCCATGATGGTGTCTTCATCGCCTTTCTCGAAGGAGATCACGCCTTTTTTGCTGAACAGATAGGCAACGGAACCATCAGTACCGAGGTTACCGCCACATTTGCTAAATGCATTAc                                                                                                                                                     >  2:2004712/1‑150 (MQ=255)

 tCACAACGTCTTCAGCACCTGCTTCCAGTGCTGCTTCCATGATGGTGTCTTCATCGCCTTTCTCGAAGGAGATCACGCCTTTTTTGCTGAACAGATAGGCAACGGAACCATCAGTACCGAGGTTACCGCCACATTTGCTAAATGCATTAc                                                                                                                                                     <  1:1181366/150‑1 (MQ=255)

    caACGTCTTCAGCACCTGCTTCCAGTGCTGCTTCCATGATGGTGTCTTCATCGCCTTTCTCGAAGGAGATCACGCCTTTTTTGCTGAACAGATAGGCAACGGAACCATCAGTACCGAGGTTACCGCCACATTTGCTAAATGCATGACGCa                                                                                                                                                  <  2:3542574/150‑1 (MQ=255)

     aaCGTCTTCAGCACCTGCTTCCAGTGCTGCTTCCATGATGGTGTCTTCATCGCCTTTCTCGAAGGAGATCACGCCTTTTTTGCTGAACAGATAGGCAACGGAACCATCAGTACCGAGGTTACCGCCACATTTGCTAAATGCATTACGCAc                                                                                                                                                 >  2:3014502/1‑150 (MQ=255)

        gTCTTCAGCACCTGCTTCCAGTGCTGCTTCCATGATGGTGTCTTCATCGCCTTTCTCGAAGGAGATCACGCCTTTTTTGCTGAACAGATAGGCAACGGAACCATCAGTACCGAGGTTACCGCCACATTTGCTAAATGCATTACGCACTTc                                                                                                                                              <  2:1553519/150‑1 (MQ=255)

         tCTTCAGCACCTGCTTCCAGTGCTGCTTCCATGATGGTGTCTTCATCGCCTTTCTCGAAGGAGATCACGCCTTTTTTGCTGAACAGATAGGCAACGGAACCATCAGTACCGAGGTTACCGCCACATTTGCTAAATGCATGACGCACTTCa                                                                                                                                             <  1:1711458/150‑1 (MQ=255)

         tCTTCAGCACCTGCTTCCAGTGCTGCTTCCATGATGGTGTCTTCATCGCCTTTCTCGAAGGAGATCACGCCTTTTTTGCTGAACAGATAGGCAACGGAACCATCAGTACCGAGGTTACCGCCACATTTGCTAAATGCATGACGCACTTCa                                                                                                                                             >  1:4580409/1‑150 (MQ=255)

           ttCAGCACCTGCTTCCAGTGCTGCTTCCATGATGGTGTCTTCATCGCCTTTCTCGAAGGAGATCACGCCTTTTTTGCTGAACAGATAGGCAACGGAACCATCAGTACCGAGGTTACCGCCACATTTGCTAAATGCATGACGCACTTCAGc                                                                                                                                           >  1:2122196/1‑150 (MQ=255)

              aGCACCTGCTTCCAGTGCTGCTTCCATGATGGTGTCTTCATCGCCTTTCTCGAAGGAGATCACGCCTTTTTTGCTGAACAGATAGGCAACGGAACCATCAGTACCGAGGTTACCGCCACATTTGCTAAATGCATGACGCACTTCAGCAac                                                                                                                                        <  1:2551458/150‑1 (MQ=255)

                cACCTGCTTCCAGTGCTGCTTCCATGATGGTGTCTTCATCGCCTTTCTCGAAGGAGATCACGCCTTTTTTGCTGAACAGATAGGCAACGGAACCATCAGTACCGAGGTTACCGCCACATTTGCTAAATGCATGACGCACTTCAGCAacgg                                                                                                                                      >  1:238834/1‑150 (MQ=255)

                 aCCTGCTTCCAGTGCTGCTTCCATGATGGTGTCTTCATCGCCTTTCTCGAAGGAGATCACGCCTTTTTTGCTGAACAGATAGGCAACGGAACCATCAGTACCGAGGTTACCGCCACATTTGCTAAATGCATGACGCACTTCAGCAacggt                                                                                                                                     <  1:4858208/150‑1 (MQ=255)

                  ccTGCTTCCAGTGCTGCTTCCATGATGGTGTCTTCATCGCCTTTCTCGAAGGAGATCACGCCTTTTTTGCTGAACAGATAGGCAACGGAACCATCAGTACCGAGGTTACCGCCACATTTGCTAAATGCATTACGCACTTCAGCAacggta                                                                                                                                    <  1:3231069/150‑1 (MQ=255)

                   cTGCTTCCAGTGCTGCTTCCAGGATGGTGACTTCATCGCCTTTCTCGAAGGAGATCACGCCTTTTTTGCTGAACAGATAGGCAACGGAACCATCAGTACCGAGGTTACCGCCACATTTGCTAAATGCATGACGCACTTCAGCAacggtac                                                                                                                                   <  2:603518/150‑1 (MQ=255)

                    tGCTTCCAGTGCTGCTTCCATGATGGTGTCTTCATCGCCTTTCTCGAAGGAGATCACGCCTTTTTTGCTGAACAGATAGGCAACGGAACCATCAGTACCGAGGTTACCGCCACATTTGCTAAATGCATGACGCACTTCAGCAacggtacg                                                                                                                                  <  1:2835239/150‑1 (MQ=255)

                     gCTTCCAGTGCTGCTTCCATGATGGTGTCTTCATCGCCTTTCTCGAAGGAGATCACGCCTTTTTTGCTGAACAGATAGGCAACGGAACCATCAGTACCGAGGTTACCGCCACATTTGCTAAATGCATGACGCACTTCAGCAacggtacgg                                                                                                                                 >  1:2318251/1‑150 (MQ=255)

                                tgctTCCATGATGGTGTCTTCATCGCCTTTCTCGAAGGAGATCACGCCTTTTTTGCTGAACAGATAGGCAACGGAACCATCAGTACCGAGGTTACCGCCACATTTGCTAAATGCATTACGCACTTCAGCAACGGTACGGTTGCGGTTGTc                                                                                                                      >  1:3327264/1‑150 (MQ=255)

                                tgctTCCATGATGGTGTCTTCATCGCCTTTCTCGAAGGAGATCACGCCTTTTTTGCTGAACAGATAGGCAACGGAACCATCAGTACCGAGGTTACCGCCACATTTGCTAAATGCATTACGCACTTCAGCAACGGTACGGTTGCGGTTGTc                                                                                                                      >  1:1197211/1‑150 (MQ=255)

                                  ctTCCATGATGGTGTCTTCATCGCCTTTCTCGAAGGAGATCACGCCTTTTTTGCTGAACAGATAGGCAACGGAACCATCAGTACCGAGGTTACCGCCACATTTGCTAAATGCATGACGCACTTCAGCAACGGTACGGTTGCGGTTGTcag                                                                                                                    <  1:1490813/150‑1 (MQ=255)

                                    tCCATGATGGTGTCTTCATCGCCTTTCTCGAAGGAGATCACGCCTTTTTTGCTGAACAGATAGGCAACGGAACCATCAGTACCGAGGGTACCGCCACATTTGCTAAATGCATGACGCACTTCAGCAACGGTACGGTTGCGGTTGTcagac                                                                                                                  <  1:4669546/150‑1 (MQ=255)

                                     ccATGATGGTGTCTTCATCGCCTTTCTCGAAGGAGATCACGCCTTTTTTGCTGAACAGATAGGCAACGGAACCATCAGTACCGAGGTTACCGCCACATTTGCTAAATGCATGACGCACTTCAGCAACGGTACGGTTGCGGTTGTcagaca                                                                                                                 >  1:5046041/1‑150 (MQ=255)

                                        tgatgGTGTCTTCATCGCCTTTCTCGAAGGAGATCACGCCTTTTTTGCTGAACAGATAGGCAACGGAACCATCAGTACCGAGGTTACCGCCACATTTGCTAAATGCATGACGCACTTCAGCAACGGTACGGTTGCGGTTGTCagacagac                                                                                                              <  1:691996/150‑1 (MQ=255)

                                        tgatgGTGTCTTCATCGCCTTTCTCGAAGGAGATCACGCCTTTTTTGCTGAACAGATAGGCAACGGAACCATCAGTACCGAGGTTACCGCCACATTTGCTAAATGCATGACGCACTTCAGCAACGGTACGGTTGCGGTTGTCagacagac                                                                                                              <  1:3269031/150‑1 (MQ=255)

                                           tgGTGTCTTCATCGCCTTTCTCGAAGGAGATCACGCCTTTTTTGCTGAACAGATAGGCAACGGAACCATCAGTACCGAGGTTACCGCCACATTTGCTAAATGCATGACGCACTTCAGCAACGGTACGGTTGCGGTTGTCAGACAGACAtt                                                                                                           >  1:3524637/1‑150 (MQ=255)

                                           tgGTGTCTTCATCGCCTTTCTCGAAGGAGATCACGCCTTTTTTGCTGAACAGATAGGCAACGGAACCATCAGTACCGAGGTTACCGCCACATTTGCTAAATGCATGACGCACTTCAGCAACGGTACGGTTGCGGTTGTCAGACAGACAtt                                                                                                           <  1:4512289/150‑1 (MQ=255)

                                               gtCTTCATCGCCTTTCTCGAAGGAGATCACGCCTTTTTTGCTGAACAGATAGGCAACGGAACCATCAGTACCGAGGTTACCGCCACATTTGCTAAATGCATTACGCACTTCAGCAACGGTACGGTTGCGGTTGTCAGACAGACATTCAAt                                                                                                       >  1:1336910/1‑150 (MQ=255)

                                               gtCTTCATCGCCTTTCTCGAAGGAGATCACGCCTTTTTTGCTGAACAGATAGGCAACGGAACCATCAGTACCGAGGTTACCGCCACATTTGCTAAATGCATGACGCACTTCAGCAACGGTACGGTTGCGGTTGTCAGACAGACATTCAAt                                                                                                       <  2:3342377/150‑1 (MQ=255)

                                               gtCTTCATCGCCTTTCTCGAAGGAGATCACGCCTTTTTTGCTGAACAGATAGGCAACGGAACCATCAGTACCGAGGTTACCGCCACATTTGCTAAATGCATGACGCACTTCAGCAACGGTACGGTTGCGGTTGTCAGACAGACATTCAAt                                                                                                       <  2:3825545/150‑1 (MQ=255)

                                               gtCTTCATCGCCTTTCTCGAAGGAGATCACGCCTTTTTTGCTGAACAGATAGGCAACGGAACCATCAGTACCGAGGTTACCGCCACATTTGCTAAATGCATGACGCACTTCAGCAACGGTACGGTTGCGGTTGTCAGACAGACATTCAAt                                                                                                       >  1:1568289/1‑150 (MQ=255)

                                                tCTTCATCGCCTTTCTCGAAGGAGATCACGCCTTTTTTGCTGAACAGATAGGCAACGGAACCATCAGTACCGAGGTTACCGCCACATTTGCTAAATGCATGACGCACTTCAGCAACGGTACGGTTGCGGTTGTCAGACAGACATTCAATc                                                                                                      <  1:2632244/150‑1 (MQ=255)

                                                   tCATCGCCTTTCTCGAAGGAGATCACGCCTTTTTTGCTGAACAGATAGGCAACGGAACCATCAGTACCGAGGTTACCGCCACATTTGCTAAATGCATGACGCACTTCAGCAACGGTACGGTTGCGGTTGTCAGACAGACATTCAATCATg                                                                                                   <  2:4929855/150‑1 (MQ=255)

                                                   tCATCGCCTTTCTCGAAGGAGATCACGCCTTTTTTGCTGAACAGATAGGCAACGGAACCATCAGTACCGAGGTTACCGCCACATTTGCTAAATGCATGACGCACTTCAGCAACGGTACGGTTGCGGTTGTCAGACAGACATTCAATCATg                                                                                                   >  1:4606801/1‑150 (MQ=255)

                                                    cATCGCCTTTCTCGAAGGAGATCACGCCTTTTTTGCTGAACAGATAGGCAACGGAACCATCAGTACCGAGGTTACCGCCACATTTGCTAAATGCATGACGCACTTCAGCAACGGTACGGTTGCGGTTGTCAGACAGACATTCAATCATGa                                                                                                  >  1:303824/1‑150 (MQ=255)

                                                     aTCGCCTTTCTCGAAGGAGATCACGCCTTTTTTGCTGAACAGATAGGCAACGGAACCATCAGTACCGAGGTTACCGCCACATTTGCTAAATGCATGACGCACTTCAGCAACGGTACGGTTGCGGTTGTCAGACAGACATTCAATCATGAt                                                                                                 >  2:1235108/1‑150 (MQ=255)

                                                     aTCGCCTTTCTCGAAGGAGATCACGCCTTTTTTGCTGAACAGATAGGCAACGGAACCATCAGTACCGAGGTTACCGCCACATTTGCTAAATGCATGACGCACTTCAGCAACGGTACGGTTGCGGTTGTCAGACAGACATTCAATCATGAt                                                                                                 <  1:1401319/150‑1 (MQ=255)

                                                        gCCTTTCTCGAAGGAGATCACGCCTTTTTTGCTGAACAGATAGGCAACGGAACCATCAGTACCGAGGTTACCGCCACATTTGCTAAATGCATTACGCACTTCAGCAACGGTACGGTTGCGGTTGTCAGACAGACATTCAATCATGATtgc                                                                                              >  2:2952881/1‑150 (MQ=255)

                                                           tttCTCGAAGGAGATCACGCCTTTTTTGCTGAACAGATAGGCAACGGAACCATCAGTACCGAGGTTACCGCCACATTTGCTAAATGCATTACGCACTTCAGCAACGGTACGGTTGCGGTTGTCAGACAGACATTCAATCATGATtgccgt                                                                                           <  2:1616995/150‑1 (MQ=255)

                                                           tttCTCGAAGGAGATCACGCCTTTTTTGCTGAACAGATAGGCAACGGAACCATCAGTACCGAGGTTACCGCCACATTTGCTAAATGCATGACGCACTTCAGCAACGGTACGGTTGCGGTTGTCAGACAGACATTCAATCATGATtgccgt                                                                                           >  1:4733906/1‑150 (MQ=255)

                                                           tttCTCGAAGGAGATCACGCCTTTTTTGCTGAACAGATAGGCAACGGAACCATCAGTACCGAGGTTACCGCCACATTTGCTAAATGCATGACGCACTTCAGCAACGGTACGGTTGCGGTTGTCAGACAGACATTCAATCATGATtgccgt                                                                                           <  1:102408/150‑1 (MQ=255)

                                                            ttCTCGAAGGAGATCACGCCTTTTTTGCTGAACAGATAGGCAACGGAACCATCAGTACCGAGGTTACCGCCACATTTGCTAAATGCATTACGCACTTCAGCAACGGTACGGTTGCGGTTGTCAGACAGACATTCAATCATGATtgccgtg                                                                                          <  1:4305178/150‑1 (MQ=255)

                                                            ttCTCGAAGGAGATCACGCCTTTTTTGCTGAACAGATAGGCAACGGAACCATCAGTACCGAGGTTACCGCCACATTTGCTAAATGCATGACGCACTTCAGCAACGGTACGGTTGCGGTTGTCAGACAGACATTCAATCATGATtgccgtg                                                                                          <  1:4899119/150‑1 (MQ=255)

                                                              ctcGAAGGAGATCACGCCTTTTTTGCTGAACAGATAGGCAACGGAACCATCAGTACCGAGGTTACCGCCACATTTGCTAAATGCATTACGCACTTCAGCAACGGTACGGTTGCGGTTGTCAGACAGACATTCAATCATGATtgccgtgcc                                                                                        <  2:1734909/150‑1 (MQ=255)

                                                              ctcGAAGGAGATCACGCCTTTTTTGCTGAACAGATAGGCAACGGAACCATCAGTACCGAGGTTACCGCCACATTTGCTAAATGCATGACGCACTTCAGCAACGGTACGGTTGCGGTTGTCAGACAGACATTCAATCATGATtgccgtgcc                                                                                        <  1:106258/150‑1 (MQ=255)

                                                               tcGAAGGAGATCACGCCTTTTTTGCTGAACAGATAGGCAACGGAACCATCAGTACCGAGGTTACCGCCACATTTGCTAAATGCATGACGCACTTCAGCAACGGTACGGTTGCGGTTGTCAGACAGACATTCAATCATGATtgccgtgccg                                                                                       <  1:2289422/150‑1 (MQ=255)

                                                                cGAAGGAGATCACGCCTTTTTTGCTGAACAGATAGGCAACGGAACCATCAGTACCGAGGTTACCGCCACATTTGCTAAATGCATGACGCACTTCAGCAACGGTACGGTTGCGGTTGTCAGACAGACATTCAATCATGATTGCCCTgccgc                                                                                      >  1:3607122/1‑150 (MQ=255)

                                                                 gAAGGAGATCACGCCTTTTTTGCTGAACAGATAGGCAACGGAACCATCAGTACCGAGGTTACCGCCACATTTGCTAAATGCATGACGCACTTCAGCAACGGTACGGTTGCGGTTGTCAGACAGACATTCAATCATGATTGCCGTgccgcc                                                                                     <  1:1765008/150‑1 (MQ=255)

                                                                  aaGGAGATCACGCCTTTTTTGCTGAACAGATAGGCTACGGTACCATCAGTTCCGAGGTTACCGCCACATTTGCTACATGCATGACGCACTTCAGCAACGGTACGGTTGCTGTTGTCAGACAGACATTCACTCATGATTGCCGTGACGCCa                                                                                    <  2:2628530/150‑1 (MQ=255)

                                                                  aaGGAGATCACGCCTTTTTTGCTGAACAGATAGGCAACGGAACCATCAGTACCGAGGTTACCGCCACATTTGCTAAATGCATGACGCACTTCAGCAACGGTACGGTTGCGGTTGTCAGACAGACATTCAATCATGATTGCCGTGCCGCCa                                                                                    <  2:3680660/150‑1 (MQ=255)

                                                                   aGGAGATCACGCCTTTTTTGCTGAACAGATAGGCAACGGAACCATCAGTACCGAGGTTACCGCCACATTTGCTAAATGCATTACGCACTTCAGCAACGGTACGGTTGCGGTTGTCAGACAGACATTCAATCATGATTGCCGTGCCGCCAg                                                                                   <  1:4208357/150‑1 (MQ=255)

                                                                   aGGAGATCACGCCTTTTTTGCTGAACAGATAGGCAACGGAACCATCAGTACCGAGGTTACCGCCACATTTGCTAAATGCATGACGCACTTCAGCAACGGTACGGTTGCGGTTGTCAGACAGACATTCAATCATGATTGCCGTGCCGCCAg                                                                                   >  1:5118423/1‑150 (MQ=255)

                                                                        aTCACGCCTTTTTTGCTGAACAGATAGGCAACGGAACCATCAGTACCGAGGTTACCGCCACATTTGCTAAATGCATGACGCACTTCAGCAACGGTACGGTTGCGGTTGTCAGACAGACATTCAATCATGATTGCCGTGCCGCCAGGACCg                                                                              >  2:2180548/1‑150 (MQ=255)

                                                                            cGCCTTTTTTGCTGAACAGATAGGCAACGGAACCATCAGTACCGAGGTTACCGCCACATTTGCTAAATGCATGACGCACTTCAGCAACGGTACGGTTGCGGTTGTCAGACAGACATTCAATCATGATTGCCGTGCCGCCAGGACCGTAAc                                                                          <  1:2901084/150‑1 (MQ=255)

                                                                              ccTTTTTTGCTGAACAGATAGGCAACGGAACCATCAGTACCGAGGTTACCGCCACATTTGCTAAATGCATTACGCACTTCAGCAACGGTACGGTTGCGGTTGTCAGACAGACATTCAATCATGATTGCCGTGCCGCCAGGACCGTAACCt                                                                        >  1:2732877/1‑150 (MQ=255)

                                                                               cTTTTTTGCTGAACAGATAGGCAACGGAACCATCAGTACCGAGGTTACCGCCACATTTGCTAAATGCATGACGCACTTCAGCAACGGTACGGTTGCGGTTGTCAGACAGACATTCAATCATGATTGCCGTGCCGCCAGGACCGTAACCtt                                                                       >  1:955774/1‑150 (MQ=255)

                                                                                  ttttGCTGAACAGATAGGCAACGGAACCATCAGTACCGAGGTTACCGCCACATTTGCTAAATGCATGACGCACTTCAGCAACGGTACGGTTGCGGTTGTCAGACAGACATTCAATCATGATTGCCGTGCCGCCAGGACCGTAACCTTCGt                                                                    <  1:1794988/150‑1 (MQ=255)

                                                                                   tttGCTGAACAGATAGGCAACGGAACCATCAGTACCGAGGTTACCGCCACATTTGCTAAATGCATGACGCACTTCAGCAACGGTACGGTTGCGGTTGTCAGACAGACATTCAATCATGATTGCCGTGCCGCCAGGACCGTAACCTTCGTa                                                                   >  1:375346/1‑150 (MQ=255)

                                                                                   tttGCTGAACAGATAGGCAACGGAACCATCAGTACCGAGGTTACCGCCACATTTGCTAAATGCATGACGCACTTCAGCAACGGTACGGTTGCGGTTGTCAGACAGACATTCAATCATGATTGCCGTGCCGCCAGGACCGTAACCTTCGTa                                                                   <  1:3315852/150‑1 (MQ=255)

                                                                                        tGAACAGATAGGCAACGGAACCATCAGTACCGAGGTTACCGCCACATTTGCTAAATGCATGACGCACTTCAGCAACGGTACGGTTGCGGTTGTCAGACAGACATTCAATCATGATTGCCGTGCCGCCAGGACCGTAACCTTCGTAgatga                                                              >  2:48572/1‑150 (MQ=255)

                                                                                         gAACAGATAGGCAACGGAACCATCAGTACCGAGGTTACCGCCACATTTGCTAAATGCATTACGCACTTCAGCAACGGTACGGTTGCGGTTGTCAGACAGACATTCAATCATGATTGCCGTGCCGCCAGGACCGTAACCTTCGTAgatgat                                                             >  2:1845766/1‑150 (MQ=255)

                                                                                           aCAGATAGGCAACGGAACCATCAGTACCGAGGTTACCGCCACATTTGCTAAATGCATGACGCACTTCAGCAACGGTACGGTTGCGGTTGTCAGACAGACATTCAATCATGATTGCCGTGCCGCCAGGACCGTAACCTTCGTAGATGATgg                                                           <  1:3458795/150‑1 (MQ=255)

                                                                                             aGATAGGCAACGGAACCATCAGTACCGAGGTTACCGCCACATTTGCTAAATGCATTACGCACTTCAGCAACGGTACGGTTGCGGTTGTCAGACAGACATTCAATCATGATTGCCGTGCCGCCAGGACCGTAACCTTCGTAGATGATGGtt                                                         >  2:2805510/1‑150 (MQ=255)

                                                                                             aGATAGGCAACGGAACCATCAGTACCGAGGTTACCGCCACATTTGCTAAATGCATTACGCACTTCAGCAACGGTACGGTTGCGGTTGTCAGACAGACATTCAATCATGATTGCCGTGCCGCCAGGACCGTAACCTTCGTAGATGATGGtt                                                         >  2:2697215/1‑150 (MQ=255)

                                                                                             aGATAGGCAACGGAACCATCAGTACCGAGGTTACCGCCACATTTGCTAAATGCATGACGCACTTCAGCAACGGTACGGTTGCGGTTGTCAGACAGACATTCAATCATGATTGCCGTGCCGCCAGGACCGTAACCTTCGTAGATGATGGtt                                                         >  1:853090/1‑150 (MQ=255)

                                                                                             aGATAGGCAACGGAACCATCAGTACCGAGGGTACCGCCACATTTGCTAAATGCATGACGCACTTCAGCAACGGTACGGTTGCGGTTGTCAGACAGACATTCAATCATGATTGCCGTGCCGCCAGGACCGTAACCTTCGTAGATGATGGtt                                                         <  2:797297/150‑1 (MQ=255)

                                                                                                tAGGCAACGGAACCATCAGTACCGAGGTTACCGCCACATTTGCTAAATGCATTACGCACTTCAGCAACGGTACGGTTGCGGTTGTCAGACAGACATTCAATCATGATTGCCGTGCCGCCAGGACCGTAACCTTCGTAGATGATGGTTTcc                                                      <  2:4457571/150‑1 (MQ=255)

                                                                                                tAGGCAACGGAACCATCAGTACCGAGGTTACCGCCACATTTGCTAAATGCATTACGCACTTCAGCAACGGTACGGTTGCGGTTGTCAGACAGACATTCAATCATGATTGCCGTGCCGCCAGGACCGTAACCTTCGTAGATGATGGTTTcc                                                      <  2:3221284/150‑1 (MQ=255)

                                                                                                tAGGCAACGGAACCATCAGTACCGAGGTTACCGCCACATTTGCTAAATGCATGACGCACTTCAGCAACGGTACGGTTGCGGTTGTCAGACAGACATTCAATCATGATTGCCGTGCCGCCAGGACCGTAACCTTCGTAGATGATGGTTTcc                                                      <  2:2122245/150‑1 (MQ=255)

                                                                                                 aGGCAACGGAACCATCAGTACCGAGGTTACCGCCACATTTGCTAAATGCATGACGCACTTCAGCAACGGTACGGTTGCGGTTGTCAGACAGACATTCAATCATGATTGCCGTGCCGCCAGGACCGTAACCTTCGTAGATGATGGTTTCCa                                                     <  1:1884429/150‑1 (MQ=255)

                                                                                                  ggCAACGGAACCATCAGTACCGAGGTTACCGCCACATTTGCTAAATGCATTACGCACTTCAGCAACGGTACGGTTGCGGTTGTCAGACAGACATTCAATCATGATTGCCGTGCCGCCAGGACCGTAACCTTCGTAGATGATGGTTTCCAt                                                    >  2:1332253/1‑150 (MQ=255)

                                                                                                  ggCAACGGAACCATCAGTACCGAGGTTACCGCCACATTTGCTAAATGCATGACGCACTTCAGCAACGGTACGGTTGCGGTTGTCAGACAGACATTCAATCATGATTGCCGTGCCGCCAGGACCGTAACCTTCGTAGATGATGGTTTCCAt                                                    <  2:1468319/150‑1 (MQ=255)

                                                                                                  ggCAACGGAACCATCAGTACCGAGGTTACCGCCACATTTGCTAAATGCATGACGCACTTCAGCAACGGTACGGTTGCGGTTGTCAGACAGACATTCAATCATGATTGCCGTGCCGCCAGGACCGTAACCTTCGTAGATGATGGTTTCCAt                                                    >  1:3398862/1‑150 (MQ=255)

                                                                                                  ggCAACGGAACCATCAGTACCGAGGTTACCGCCACATTTGCTAAATGCATGACGCACTTCAGCAACGGTACGGTTGCGGTTGTCAGACAGACATTCAATCATGATTGCCGTGCCGCCAGGACCGTAACCTTCGTAGATGATGGTTTCCAt                                                    <  2:639763/150‑1 (MQ=255)

                                                                                                     aaCGGAACCATCAGTACCGAGGTTACCGCCACATTTGCTAAATGCATTACGCACTTCAGCAACGGTACGGTTGCGGTTGTCAGACAGACATTCAATCATGATTGCCGTGCCGCCAGGACCGTAACCTTCGTAGATGATGGTTTCCATGtt                                                 >  2:2471184/1‑150 (MQ=255)

                                                                                                     aaCGGAACCATCAGTACCGAGGTTACCGCCACATTTGCTAAATGCATGACGCACTTCAGCAACGGTACGGTTGCGGTTGTCAGACAGACATTCAATCATGATTGCCGTGCCGCCAGGACCGTAACCTTCGTAGATGATGGTTTCCATGtt                                                 >  1:3974065/1‑150 (MQ=255)

                                                                                                     aaCGGAACCATCAGTACCGAGGTTACCGCCACATTTGCTAAATGCATGACGCACTTCAGCAACGGTACGGTTGCGGTTGTCAGACAGACATTCAATCATGATTGCCGTGCCGCCAGGACCGTAACCTTCGTAGATGATGGTTTCCATGtt                                                 >  2:2977021/1‑150 (MQ=255)

                                                                                                       cGGAACCATCAGTACCGAGGTTACCGCCACATTTGCTAAATGCATTACGCACTTCAGCAACGGTACGGTTGCGGTTGTCAGACAGACATTCAATCATGATTGCCGTGCCGCCAGGACCGTAACCTTCGTAGATGATGGTTTCCATGTTTg                                               <  1:819343/150‑1 (MQ=255)

                                                                                                        ggAACCATCAGTACCGAGGTTACCGCCACATTTGCTAAATGCATTACGCACTTCAGCAACGGTACGGTTGCGGTTGTCAGACAGACATTCAATCATGATTGCCGTGCCGCCAGGACCGTAACCTTCGTAGATGATGGTTTCCATGTTTGc                                              >  2:2291012/1‑150 (MQ=255)

                                                                                                             cATCAGTACCGAGGTTACCGCCACATTTGCTAAATGCATTACGCACTTCAGCAACGGTACGGTTGCGGTTGTCAGACAGACATTCAATCATGATTGCCGTGCCGCCAGGACCGTAACCTTCGTAGATGATGGTTTCCATGTTTGcatcat                                         >  2:4372972/1‑150 (MQ=255)

                                                                                                             cATCAGTACCGAGGTTACCGCCACATTTGCTAAATGCATTACGCACTTCAGCAACGGTACGGTTGCGGTTGTCAGACAGACATTCAATCATGATTGCCGTGCCGCCAGGACCGTAACCTTCGTAGATGATGGTTTCCATGTTTGcatcat                                         >  2:4894980/1‑150 (MQ=255)

                                                                                                               tCAGTACCGAGGTTACCGCCACATTTGCTAAATGCATGACGCACTTCAGCAACGGTACGGTTGCGGTTGTCAGACAGACATTCAATCATGATTGCCGTGCCGCCAGGACCGTAACCTTCGTAGATGATGGTTTCCATGTTTGcatcatca                                       >  1:4321082/1‑150 (MQ=255)

                                                                                                               tCAGTACCGAGGTTACCGCCACATTTGCTAAATGCATGACGCACTTCAGCAACGGTACGGTTGCGGTTGTCAGACAGACATTCAATCATGATTGCCGTGCCGCCAGGACCGTAACCTTCGTAGATGATGGTTTCCATGTTTGcatcatca                                       <  2:4681655/150‑1 (MQ=255)

                                                                                                               tCAGTACCGAGGTTAACGCCACATTAGCTAAATGCATGAAGAACTTCAGCAACGGTACGGATGCGGTTGTAAGACAGACATTCAATCATCATTGCCGAGCCGCCAGGACCGTAACCTTCGTAGATGATGGTTTCCAAGATTGcataatca                                       >  2:3865619/1‑150 (MQ=255)

                                                                                                                cAGTACCGAGGTTACCGCCACATTTGCTAAATGCATGACGCACTTCAGCAACGGTACGGTTGCGGTTGTCAGACAGACATTCAATCATGATTGCCGTGCCGCCAGGACCGTAACCTTCGTAGATGATGGTTTCCATGTTTGcatcatcat                                      >  2:2414501/1‑150 (MQ=255)

                                                                                                                      cGAGGTTACCGCCACATTTGCTAAATGCATGACGCACTTCAGCAACGGTACGGTTGCGGTTGTCAGACAGACATTCAATCATGATTGCCGTGCCGCCAGGACCGTAACCTTCGTAGATGATGGTTTCCATGTTTGCATCATCATCACCGc                                <  2:4515187/150‑1 (MQ=255)

                                                                                                                        aGGTTACCGCCACATTTGCTAAATGCATTACGCACTTCAGCAACGGTACGGTTGCGGTTGTCAGACAGACATTCAATCATGATTGCCGTGCCGCCAGGACCGTAACCTTCGTAGATGATGGTTTCCATGTTTGCATCATCATCACCGccc                              <  2:190484/150‑1 (MQ=255)

                                                                                                                          gTTACCGCCACATTTGCTAAATGCATTACGCACTTCAGCAACGGTACGGTTGCGGTTGTCAGACAGACATTCAATCATGATTGCCGTGCCGCCAGGACCGTAACCGTCGTAGATGATGGTTTCCATGTTTGCATCATCATCACCGCCcac                            >  1:4460595/1‑150 (MQ=255)

                                                                                                                           ttACCGCCACATTTGCTAAATGCATTACGCACTTCAGCAACGGTACGGTTGCGGTTGTCAGACAGACATTCAATCATGATTGCCGTGCCGCCAGGACCGTAACCTTCGTAGATGATGGTTTCCATGTTTGCATCATCATCACCGCCcaca                           >  2:3933425/1‑150 (MQ=255)

                                                                                                                            tACCGCCACATTTGCTAAATGCATTACGCACTTCAGCAACGGTACGGTTGCGGTTGTCAGACAGACATTCAATCATGATTGCCGTGCCGCCAGGACCGTAACCTTCGTAGATGATGGTTTCCATGTTTGCATCATCATCACCGCCCacac                          >  1:2600057/1‑150 (MQ=255)

                                                                                                                            tACCGCCACATTTGCTAAATGCATTACGCACTTCAGCAACGGTACGGTTGCGGTTGTCAGACAGACATTCAATCATGATTGCCGTGCCGCCAGGACCGTAACCTTCGTAGATGATGGTTTCCATGTTTGCATCATCATCACCGCCCacac                          >  1:502357/1‑150 (MQ=255)

                                                                                                                            tACCGCCACATTTGCTAAATGCATGACGCACTTCAGCAACGGTACGGTTGCGGTTGTCAGACAGACATTCAATCATGATTGCCGTGCCGCCAGGACCGTAACCTTCGTAGATGATGGTTTCCATGTTTGCATCATCATCACCGCCCacac                          >  1:1242962/1‑150 (MQ=255)

                                                                                                                               cGCCACATTTGCTAAATGCATGACGCACTTCAGCAACGGTACGGTTGCGGTTGTCAGACAGACATTCAATCATGATTGCCGTGCCGCCAGGACCGTAACCTTCGTAGATGATGGTTTCCATGTTTGCATCATCATCACCGCCCAcaccac                       <  1:4190575/150‑1 (MQ=255)

                                                                                                                                     aTTTGCTAAATGCATGACGCACTTCAGCAACGGTACGGTTGCGGTTGTCAGACAGACATTCAATCATGATTGCCGTGCCGCCAGGACCGTAACCTTCGTAGATGATGGTTTCCATGTTTGCATCATCATCACCGCCCACACCACGTGCaa                 <  1:1648033/150‑1 (MQ=255)

                                                                                                                                     aTTTGCTAAATGCATGACGCACTTCAGCAACGGTACGGTTGCGGTTGTCAGACAGACATTCAATCAAGATTGCCGTGCCGCCAGGACCGAAACCTTCGTAGATGATGGTTTCAATGTTTGCATCATCAACACCGCCCACACCACGTGCaa                 >  2:4564363/1‑150 (MQ=255)

                                                                                                                                        tGCTAAATGCATGACGCACTTCAGCAACGGTACGGTTGCGGTTGTCAGACAGACATTCAATCATGATTGCCGTGCCGCCAGGACCGTAACCTTCGTAGATGATGGTTTCCATGTTTGCATCATCATCACCGCCCACACCACGTGCAATTg              <  2:1940744/150‑1 (MQ=255)

                                                                                                                                         gCTAAATGCATTACGCACTTCAGCAACGGTACGGTTGCGGTTGTCAGACAGACATTCAATCATGATTGCCGTGCCGCCAGGACCGTAACCTTCGTAGATGATGGTTTCCATGTTTGCATCATCATCACCGCCCACACCACGTGCAATTgc             <  1:3014502/150‑1 (MQ=255)

                                                                                                                                         gCTAAATGCATGACGCACTTCAGCAACGGTACGGTTGCGGTTGTCAGACAGACATTCAATCATGATTGCCGTGCCGCCAGGACCGTAACCTTCGTAGATGATGGTTTCCATGTTTGCATCATCATCACCGCCCACACCACGTGCAATTgc             >  2:3591711/1‑150 (MQ=255)

                                                                                                                                               tGCATGACGCACTTCAGCAACGGTACGGTTGCGGTTGTCAGACAGACATTCAATCATGATTGCCGTGCCGCCAGGACCGTAACCTTCGTAGATGATGGTTTCCATGTTTGCATCATCATCACCGCCCACACCACGTGCAATTGCGCGGtt       >  1:3167313/1‑150 (MQ=255)

                                                                                                                                               tGCATGACGCACTTCAGCAACGGTACGGTTGCGGTTGTCAGACAGACATTCAATCATGATTGCCGTGCCGCCAGGACCGTAACCTTCGTAGATGATGGTTTCCATGTTTGCATCATCATCACCGCCCACACCACGTGCAATTGCGCGGtt       >  2:4189908/1‑150 (MQ=255)

                                                                                                                                                   tGACGCACTTCAGCAACGGTACGGTTGCGGTTGTCAGACAGACATTCAATCATGATTGCCGTGCCGCCAGGACCGTAACCTTCGTAGATGATGGTTTCCATGTTTGCATCATCATCACCGCCCACACCACGTGCAATTGCGCGGTTCAgt   >  2:4288422/1‑150 (MQ=255)

                                                                                                                                                    gACGCACTTCAGCAACGGTACGGTTGCGGTTGTCAGACAGACATTCAATCATGATTGCCGTGCCGCCAGGACCGTAACCTTCGTAGATGATGGTTTCCATGTTTGCATCATCATCACCGCCCACACCACGTGCAATTGCGCGGTTCAgtg  <  1:4250583/150‑1 (MQ=255)

GTCACAACGTCTTCAGCACCTGCTTCCAGTGCTGCTTCCATGATGGTGTCTTCATCGCCTTTCTCGAAGGAGATCACGCCTTTTTTGCTGAACAGATAGGCAACGGAACCATCAGTACCGAGGTTACCGCCACATTTGCTAAATGCATGACGCACTTCAGCAACGGTACGGTTGCGGTTGTCAGACAGACATTCAATCATGATTGCCGTGCCGCCAGGACCGTAACCTTCGTAGATGATGGTTTCCATGTTTGCATCATCATCACCGCCCACACCACGTGCAATTGCGCGGTTCAGTG  >  CP009273/1941918‑1942215

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`