Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,991,843 G→A 26.5% L40F (CTC→TTC)  yecS ← ABC family putative inner membrane permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,991,8430GA26.5% 606.7 / 258.8 306L40F (CTC→TTC) yecSABC family putative inner membrane permease
Reads supporting (aligned to +/- strand):  ref base G (108/117);  new base A (28/53);  total (136/170)
Fisher's exact test for biased strand distribution p-value = 3.81e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 310 total aligned reads displayed.

CGGAATGGGATCTAATTCGATACCAAACTGCGGCAGGCCGTAATAGATCATAAACAGTTGTGCGATGAGCGGCGTACCACGAAAAATAGAGATATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGTGGGGATGA  >  CP009273/1991694‑1991990
                                                                                                                                                     |                                                                                                                                                   
cGGAATGGGATCTAATTCGATACCAAACTGCGGCAGGCCGTAATAGATCATAAACAGTTGTGCGATGAGCGGCGTACCACGAAAAATAGAGATATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCgag                                                                                                                                                     <  1:3026087/150‑1 (MQ=255)
    aTGGGATCTAATTCGATACCAAACTGCGGCAGGCCGTAATAGATCATAAACAGTTGTGCGATGAGCGGCGTACCACGAAAAATAGAGATATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATa                                                                                                                                                 <  2:673176/150‑1 (MQ=255)
     tGGGATCTAATTCGATACCAAACTGCGGCAGGCCGTAATAGATCATAAACAGTTGTGCGATGAGCGGCGTACCACGAAAAATAGAGATATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATaa                                                                                                                                                >  2:4886974/1‑150 (MQ=255)
       ggATCTAATTCGATACCAAACTGCGGCAGGCCGTAATAGATCATAAACAGTTGTGCGATGAGCGGCGTACCACGAAAAATAGAGATATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAAAATAAAc                                                                                                                                              >  2:1194116/1‑150 (MQ=255)
          tCTAATTCGATACCAAACTGCGGCAGGCCGTAATAGATCATAAACAGTTGTGCGATGAGCGGCGTACCACGAAAAATAGAGATATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAAAATAAACCCg                                                                                                                                           <  1:4234982/150‑1 (MQ=255)
           cTAATTCGATACCAAACTGCGGCAGGCCGTAATAGATCATAAACAGTTGTGCGATGAGCGGCGTACCACGAAAAATAGAGATATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGa                                                                                                                                          >  1:329841/1‑150 (MQ=255)
           cTAATTCGATACCAAACTGCGGCAGGCCGTAATAGATCATAAACAGTTGTGCGATGAGCGGCGTACCACGAAAAATAGAGATATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGa                                                                                                                                          <  1:4155604/150‑1 (MQ=255)
            tAATTCGATACCAAACTGCGGCAGGCCGTAATAGATCATAAACAGTTGTGCGATGAGCGGCGTACCACGAAAAATAGAGATATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAg                                                                                                                                         >  1:3130339/1‑150 (MQ=255)
            tAATTCGATACCAAACTGCGGCAGGCCGTAATAGATCATAAACAGTTGTGCGATGAGCGGCGTACCACGAAAAATAGAGATATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAg                                                                                                                                         <  1:4924659/150‑1 (MQ=255)
             aaTTCGATACCAAACTGCGGCAGGCCGTAATAGATCATAAACAGTTGTGCGATGAGCGGCGTACCACGAAAAATAGAGATATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAGc                                                                                                                                        >  1:1303084/1‑150 (MQ=255)
             aaTTCGATACCAAACTGCGGCAGGCCGTAATAGATCATAAACAGTTGTGCGATGAGCGGCGTACCACGAAAAATAGAGATATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAGc                                                                                                                                        <  2:1302720/150‑1 (MQ=255)
              aTTCGATACCAAACTGCGGCAGGCCGTAATAGATCATAAACAGTTGTGCGATGAGCGGCGTACCACGAAAAATAGAGATATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAGCa                                                                                                                                       >  2:2494833/1‑150 (MQ=255)
                  gATACCAAACTGCGGCAGGCCGTAATAGATCATAAACAGTTGTGCGATGAGCGGCGTACCACGAAAAATAGAGATATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAGCAGTaa                                                                                                                                   <  1:2925247/150‑1 (MQ=255)
                   aTACCAAACTGCGGCAGGCCGTAATAGATCATAAACAGTTGTGCGATGAGCGGCGTACCACGAAAAATAGAGATATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAAAATAAACCCGAGCAGTAAg                                                                                                                                  <  1:1077853/150‑1 (MQ=255)
                     aCCAAACTGCGGCAGGCCGTAATAGATCATAAACAGTTGTGCGATGAGCGGCGTACCACGAAAAATAGAGATATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGcc                                                                                                                                >  1:3763196/1‑150 (MQ=255)
                        aaaCTGCGGCAGGCCGTAATAGATAAAAAACAGTTTTTCGATGAGCGGCGAACCACGAAAAATAGAGATATAAAAACTCGCCAGCCAGCGCACGGCCCAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCCaaa                                                                                                                             >  2:2010927/1‑150 (MQ=255)
                         aaCTGCGGCAGGCCGTAATAGATCATAAACAGTTGTGCGATGAGCGGCGTACCACGAAAAATAGAGATATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCCaaaa                                                                                                                            <  2:518148/150‑1 (MQ=255)
                         aaCTGCGGCAGGCCGTAATAGATCATAAACAGTTGTGCGATGAGCGGCGTACCACGAAAAATAGAGATATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCCaaaa                                                                                                                            >  2:957445/1‑150 (MQ=255)
                           cTGCGGCAGGCCGTAATAGATCATAAACAGTTGTGCGATGAGCGGCGTACCACGAAAAATAGAGATATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCCaaaaaa                                                                                                                          >  1:1806289/1‑150 (MQ=255)
                            tGCGGCAGGCCGTAATAGATCATAAACAGTTGTGCGATGAGCGGCGTACCACGAAAAATAGAGATATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCCAAAAAAc                                                                                                                         >  2:4826677/1‑150 (MQ=255)
                             gCGGCAGGCCGTAATAGATCATAAACAGTTGTGCGATGAGCGGCGTACCACGAAAAATAGAGATATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAAAATAAACCCGAGCAGTAAGCCAAAAAACa                                                                                                                        <  2:30519/150‑1 (MQ=255)
                              cGGCAGGCCGTAATAGATCATAAACAGTTGTGCGATGAGCGGCGTACCACGAAAAATAGAGATATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCCACAAAACAt                                                                                                                       >  2:4429699/1‑150 (MQ=255)
                              cGGCAGGCCGTAATAGATCATAAACAGTTGTGCGATGAGCGGCGTACCACGAAAAATAGAGATATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCCAAAAAACAt                                                                                                                       >  1:854295/1‑150 (MQ=255)
                                 cAGGCCGTAATAGATCATAAACAGTTGTGCGATGAGCGGCGTACCACGAAAAATAGAGATATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCCAAAAAACATgcc                                                                                                                    <  1:1408637/150‑1 (MQ=255)
                                 cAGGCCGTAATAGATCATAAACAGTTGTGCGATGAGCGGCGTACCACGAAAAATAGAGATATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCCAAAAAACATgcc                                                                                                                    <  2:570161/150‑1 (MQ=255)
                                 cAGGCCGTAATAGATCATAAACAGTTGTGCGATGAGCGGCGTACCACGAAAAATAGAGATATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCCAAAAAACATgcc                                                                                                                    <  1:2288910/150‑1 (MQ=255)
                                 cAGGCCGTAATAGATCATAAACAGTTGTGCGATGAGCGGCGTACCACGAAAAATAGAGATATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCCAAAAAACATgcc                                                                                                                    <  2:4570263/150‑1 (MQ=255)
                                  aGGCCGTAATAGATCATAAACAGTTGTGCGATGAGCGGCGTACCACGAAAAATAGAGATATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCCAAAAAACATgccg                                                                                                                   >  1:4820949/1‑150 (MQ=255)
                                    gCCGTAATAGATCATAAACAGTTGTGCGATGAGCGGCGTACCACGAAAAATAGAGATATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCCAAAAAACATgccgcc                                                                                                                 <  2:4986201/150‑1 (MQ=255)
                                      cGTAATAGATCATAAACAGTTGTGCGATGAGCGGCGTACCACGAAAAATAGAGATATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGa                                                                                                               <  2:3538764/150‑1 (MQ=255)
                                      cGTAATAGATCATAAACAGTTGTGCGATGAGCGGCGTACCACGAAAAATAGAGATATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGa                                                                                                               >  1:2640696/1‑150 (MQ=255)
                                      cGTAATAGATCATAAACAGTTGTGCGATGAGCGGCGTACCACGAAAAATAGAGATATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAAAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGa                                                                                                               <  2:446832/150‑1 (MQ=255)
                                       gTAATAGATCATAAACAGTTGTGCGATGAGCGGCGTACCACGAAAAATAGAGATATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGAt                                                                                                              >  1:2164882/1‑150 (MQ=255)
                                        tAATAGATCATAAACAGTTGTGCGATGAGCGGCGTACCACGAAAAATAGAGATATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATa                                                                                                             >  1:1731640/1‑150 (MQ=255)
                                         aaTAGATCATAAACTGTTGTGCGATGAGCGGCGTACCACGAAAAATAGAGATATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATAc                                                                                                            <  1:4070453/150‑1 (MQ=255)
                                         aaTAGATCATAAACAGTTGTGCGATGAGCGGCGTACCACGAAAAATAGAGATATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATAc                                                                                                            <  1:1678923/150‑1 (MQ=255)
                                             gATCATAAACAGTTGTGCGATGAGCGGCGTACCACGAAAAATAGAGATATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAg                                                                                                        >  1:4127040/1‑150 (MQ=255)
                                                  tAAACAGTTGTGCGATGAGCGGCGTACCACGAAAAATAGAGATATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCa                                                                                                   >  1:845634/1‑150 (MQ=255)
                                                   aaaCAGTTGTGCGATGAGCGGCGTACCACGAAAAATAGAGATATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAg                                                                                                  <  1:1133126/150‑1 (MQ=255)
                                                    aaCAGTTGTGCGATGAGCGGCGTACCACGAAAAATAGAGATATAAAAACGCGCCAGCCAGCGCACGGGCCCGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGc                                                                                                 >  2:3286060/1‑150 (MQ=255)
                                                    aaCAGTTGTGCGATGAGCGGCGTACCACGAAAAATAGAGATATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAAAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGc                                                                                                 <  1:1271210/150‑1 (MQ=255)
                                                       aGTTGTGCGATGAGCGGCGTACCACGAAAAATAGAGATATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGta                                                                                              >  2:2928082/1‑150 (MQ=255)
                                                             gCGATGAGCGGCGTACCACGAAAAATAGAGATATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCa                                                                                        >  1:268563/1‑150 (MQ=255)
                                                               gATGAGCGGCGTACCACGAAAAATAGAGATATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAAAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGc                                                                                      <  1:2900493/150‑1 (MQ=255)
                                                                   aGCGGCGTACCACGAAAAATAGAGATATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAAAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCt                                                                                  <  1:3189020/150‑1 (MQ=255)
                                                                    gcggcgTACCACGAAAAATAGAGATATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCtt                                                                                 <  1:578924/150‑1 (MQ=255)
                                                                      ggcgTACCACGAAAAATAGAGATATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTc                                                                               <  1:4096069/150‑1 (MQ=255)
                                                                       gcgTACCACGAAAAATAGAGATATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTca                                                                              <  1:1862584/150‑1 (MQ=255)
                                                                       gcgTACCACGAAAAATAGAGATATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTca                                                                              <  2:4309069/150‑1 (MQ=255)
                                                                        cgTACCACGAAAAATAGAGATATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAAAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTcag                                                                             <  1:4556384/150‑1 (MQ=255)
                                                                            ccACGAAAAATAGAGATATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAgg                                                                         >  2:1063946/1‑150 (MQ=255)
                                                                            ccACGAAAAATAGAGATATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAAAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAgg                                                                         >  1:1028276/1‑150 (MQ=255)
                                                                              aCGAAAAATAGAGATATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGaa                                                                       >  2:3990387/1‑150 (MQ=255)
                                                                              aCGAAAAATAGAGATATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAAAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGaa                                                                       >  1:1475722/1‑150 (MQ=255)
                                                                                gAAAAATAGAGATATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAAAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACg                                                                     <  1:500435/150‑1 (MQ=255)
                                                                                  aaaaTAGAGATATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGc                                                                   >  2:609203/1‑150 (MQ=255)
                                                                                   aaaTAGAGATATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCa                                                                  >  2:420903/1‑150 (MQ=255)
                                                                                     aTAGAGATATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAg                                                                <  2:4622979/150‑1 (MQ=255)
                                                                                      tAGAGATATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCGAgg                                                               >  1:4589941/1‑150 (MQ=255)
                                                                                       agagATATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGa                                                              >  2:2643902/1‑150 (MQ=255)
                                                                                        gagaTATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAAAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGaa                                                             <  2:1859843/150‑1 (MQ=255)
                                                                                         agaTATAAACACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAAt                                                            <  2:2416660/150‑1 (MQ=255)
                                                                                           atatAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAAAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCa                                                          >  2:789336/1‑150 (MQ=255)
                                                                                            tataAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCaa                                                         <  2:3425752/150‑1 (MQ=255)
                                                                                              taAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATa                                                       >  1:3662067/1‑150 (MQ=255)
                                                                                               aaaaaCGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATaa                                                      >  2:1652796/1‑150 (MQ=255)
                                                                                                aaaaCGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAAAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAAc                                                     <  2:1263709/150‑1 (MQ=255)
                                                                                                   aCGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAg                                                  <  1:2557182/150‑1 (MQ=255)
                                                                                                   aCGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAg                                                  >  1:1212885/1‑150 (MQ=255)
                                                                                                    cgcgCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGt                                                 >  2:4519742/1‑150 (MQ=255)
                                                                                                    cgcgCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGt                                                 >  2:2159743/1‑150 (MQ=255)
                                                                                                     gcgcCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGtt                                                >  1:2807429/1‑150 (MQ=255)
                                                                                                       gccagccaGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGt                                              >  1:2336167/1‑150 (MQ=255)
                                                                                                        ccagccagCGCACGGGCCAGATGGGCGACAGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATACCCCGTTGta                                             <  2:648335/150‑1 (MQ=255)
                                                                                                        ccagccagCGCACGGGCCAGAGTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAGCAGTACGCCCAAAAACATGCCGCCGATCCGGAGTTGCAGCGTAGACCCAGCCCCTGTCAGCAGGACCGGCACGGACTCACTAACCAGGTGta                                             >  2:3955450/1‑150 (MQ=255)
                                                                                                           gccagcGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAAAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATAc                                          >  1:4784304/1‑150 (MQ=255)
                                                                                                            ccagcGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACt                                         >  1:5130865/1‑150 (MQ=255)
                                                                                                                cgcACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCt                                     >  2:2687157/1‑150 (MQ=255)
                                                                                                                 gcACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCtt                                    >  2:3773024/1‑150 (MQ=255)
                                                                                                                  cACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTg                                   <  2:2367649/150‑1 (MQ=255)
                                                                                                                    cGGGCCAGATTGGCGACAGACGCATCAGCGCGAAAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCa                                 >  2:3531393/1‑150 (MQ=255)
                                                                                                                     gggCCAGATTGGCGACAGACGCATCAGCGCGAAAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGGTGTATACTTTCTTGCAt                                >  2:2876153/1‑150 (MQ=255)
                                                                                                                       gCCAGATTGGCGACAGACGCATCAGCGCGAAAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGa                              <  1:767576/150‑1 (MQ=255)
                                                                                                                         cAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCCGTTGTATACTTTCTTGCATGAGc                            >  1:3468951/1‑150 (MQ=255)
                                                                                                                         cAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGc                            >  2:4416623/1‑150 (MQ=255)
                                                                                                                         cAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGc                            <  1:3276649/150‑1 (MQ=255)
                                                                                                                          aGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCg                           <  1:2041261/150‑1 (MQ=255)
                                                                                                                          aGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCg                           <  1:64859/150‑1 (MQ=255)
                                                                                                                           gATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGt                          <  1:3899093/150‑1 (MQ=255)
                                                                                                                            aTTGGCGACAGACGCATCAGCGCGAAAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGtt                         <  2:2531386/150‑1 (MQ=255)
                                                                                                                             ttGGCGACAGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGttt                        <  1:726239/150‑1 (MQ=255)
                                                                                                                                     aGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGGTGTATACTTTCTTGCATGAGCGTTTCCGGAGCt                >  2:4023630/1‑150 (MQ=255)
                                                                                                                                     aGACGCATCAGCGCGAAAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCt                >  2:1558074/1‑150 (MQ=255)
                                                                                                                                          cATCAGCGCGAGAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACg           >  1:4378238/1‑150 (MQ=255)
                                                                                                                                           aTCAGCGCGAGAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGt          <  1:2783957/150‑1 (MQ=255)
                                                                                                                                             cAGCGCGAGAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGTgg        <  2:1778978/150‑1 (MQ=255)
                                                                                                                                             cAGCGCGAAAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGTgg        >  2:1408641/1‑150 (MQ=255)
                                                                                                                                              aGCGCGAGAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGTggg       >  1:1891372/1‑150 (MQ=255)
                                                                                                                                               gcgcGAAAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGTgggg      >  1:3563826/1‑150 (MQ=255)
                                                                                                                                               gcgcGAAAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGTgggg      >  1:992174/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                   
CGGAATGGGATCTAATTCGATACCAAACTGCGGCAGGCCGTAATAGATCATAAACAGTTGTGCGATGAGCGGCGTACCACGAAAAATAGAGATATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGTGGGGATGA  >  CP009273/1991694‑1991990

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑