BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	2,038,804	C→A	8.4%	P129Q (CCG→CAG)	yeeJ →	putative adhesin

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	2,038,804	0	C	A	8.4%	1140.2 / 51.7	304	P129Q (CCG→CAG)	yeeJ	putative adhesin
Reads supporting (aligned to +/- strand): ref base C (158/120); new base A (10/16); total (168/136)
Fisher's exact test for biased strand distribution p-value = 9.78e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 334 total aligned reads displayed.

CCAAAGCGTTGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCCCGCCGCCGGGTAATAGCAGTGACAACCTCGAGCAACAGATAGCCAGTACTTCACAGCAAATCGGGTCTCTGCTCGCCGAAGATATGAACAGCGAGCAAGCGGCAAATATGGCGCGTGGATGGGCCTCTTCTCAGGCTTCAGGCGCAATGACA  >  CP009273/2038655‑2038952

ccAACGCGTTGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACACGTTAGTGAAAAAAAATTAACCccgcc                                                                                                                                                      <  2:1667754/150‑1 (MQ=255)

ccAAAGCGTTGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCccgcc                                                                                                                                                      <  1:5098753/150‑1 (MQ=255)

 cAAAGCGTTGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCccgccg                                                                                                                                                     <  2:4586679/150‑1 (MQ=255)

    aGCGTTGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCccgccgccg                                                                                                                                                  <  1:254958/150‑1 (MQ=255)

     gCGTTGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTCCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCCCGCAGCCgg                                                                                                                                                 <  1:4492639/150‑1 (MQ=255)

      cGTTGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCCCGCAGCCggg                                                                                                                                                <  1:3601033/150‑1 (MQ=255)

        ttGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCCCGCCGCCGGGTa                                                                                                                                              <  2:4602272/150‑1 (MQ=255)

           ccGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCCCGCCGCCGGGTCATa                                                                                                                                           >  2:4677459/1‑150 (MQ=255)

            cGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCCCGCCGCCGGGTAATAg                                                                                                                                          <  2:3923755/150‑1 (MQ=255)

            cGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCCCGCCGCCGGGTAATAg                                                                                                                                          >  2:1404977/1‑150 (MQ=255)

            cGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTACCGGCACAAGTTAGTGAAAAAAAATTAACCCCGCCGCCGGGTAATAg                                                                                                                                          >  2:4994929/1‑150 (MQ=255)

             gAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCCCGCCGCCGGGTAATAGc                                                                                                                                         <  1:3012956/150‑1 (MQ=255)

             gAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTACCGGCACAAGTTAGTGAAAAAAAATTAACCCCGCCGCCGGGTAATAGc                                                                                                                                         >  2:1262657/1‑150 (MQ=255)

                cGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCCCGCCGCCGGGTAATAGCAGt                                                                                                                                      <  1:308509/150‑1 (MQ=255)

                  tttCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCCCGCCGCCGGGTAATAGCAGTGa                                                                                                                                    <  2:3693387/150‑1 (MQ=255)

                   ttCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCCCGCCGCCGGGTAATAGCAGTGAc                                                                                                                                   >  2:1347390/1‑150 (MQ=255)

                   ttCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCCCGCCGCCGGGTAATAGCAGTGAc                                                                                                                                   <  1:4776541/150‑1 (MQ=255)

                     ctgTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTACCGGCACAAGTTAGTGAAAAAAAATTAACCCCGCCGCCGGGTCATAGCAGTGACaa                                                                                                                                 >  2:4302972/3‑150 (MQ=255)

                     cGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCCCGCCGCCGGGTAATAGCAGTGACaa                                                                                                                                 <  1:470818/150‑1 (MQ=255)

                     cGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCCCGCCGCCGGGTAATAGCAGTGACaa                                                                                                                                 >  1:740523/1‑150 (MQ=255)

                         aTTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCCCGCCGCCGGGTAATAGCAGTGACAACCTc                                                                                                                             <  1:1664782/150‑1 (MQ=255)

                         aTTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCCCGCCGCCGGGTAATAGCAGTGACAACCTc                                                                                                                             >  2:3979425/1‑150 (MQ=255)

                         aTTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCCCGCCGCCGGGTAATAGCAGTGACAACCTc                                                                                                                             <  1:4420721/150‑1 (MQ=255)

                              ggTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCCCGCCGCCGGGTAATAGCAGTGACAACCTCGAGCa                                                                                                                        >  2:2599418/1‑150 (MQ=255)

                                  gCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCCCGCCGCCGGGTAATAGCAGTGACAACCTCGAGCAACAg                                                                                                                    <  1:1565250/150‑1 (MQ=255)

                                  gCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTACCGGCACAAGTTAGTGAAAAAAAATTAACCCCGCCGCCGGGTAATAGCAGTGACAACCTCGAGCAACAg                                                                                                                    >  1:1273075/1‑150 (MQ=255)

                                    tGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCCCGCCGCCGGGTAATAGCAGTGACAACCTCGAGCAACAGAt                                                                                                                  >  1:1865783/1‑150 (MQ=255)

                                      aGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCCCGCCGCCGGGTAATAGCAGTGACAACCTCGAGCAACAGATAg                                                                                                                <  1:3847389/150‑1 (MQ=255)

                                      aGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCCCGCCGCCGGGTAATAGCAGTGACAACCTCGAGCAACAGATAg                                                                                                                >  1:4162804/1‑150 (MQ=255)

                                      aGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCCCGCCGCCGGGTAATAGCAGTGACAACCTCGAGCAACAGATAg                                                                                                                >  2:4796274/1‑150 (MQ=255)

                                       gTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCCCGCCGCCGGGTAATAGCAGTGACAACCTCGAGCAACAGATAGc                                                                                                               >  2:1068137/1‑150 (MQ=255)

                                        ttACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCCCGCCGCCGGGTAATAGCAGTGACAACCTCGAGCAACAGATAGcc                                                                                                              <  1:1351179/150‑1 (MQ=255)

                                         tACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCCCGCCGCCGGGTAATAGCAGTGACAACCTCGAGCAACAGATAGCCa                                                                                                             <  2:1513561/150‑1 (MQ=255)

                                         tACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCCCGCCGCCGGGTAATAGCAGTGACAACCTCGAGCAACAGATAGCCa                                                                                                             <  1:167353/150‑1 (MQ=255)

                                           cGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGACAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCCCGCCGCCGGGTAATAGCAGTGACAACCTCGAGCAACAGATAGCCAGt                                                                                                           >  2:6449/1‑150 (MQ=255)

                                             cAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCCCGCCGCCGGGTAATAGCAGTGACAACCTCGAGCAACAGATAGCCAGTAc                                                                                                         >  1:4800739/1‑150 (MQ=255)

                                              aaaCTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCCCGCCGCCGGGTAATAGCAGTGACAACCTCGAGCAACAGATAGCCAGTACt                                                                                                        >  1:375860/1‑150 (MQ=255)

                                              aaaCTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCCCGCCGCCGGGTAATAGCAGTGACAACCTCGAGCAACAGATAGCCAGTACt                                                                                                        >  2:3410180/1‑150 (MQ=255)

                                               aaCTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCCCGCAGCCGGGTAATAGCAGTGACAACCTCGAGCAACAGATAGCCAGTACtt                                                                                                       <  1:4280042/150‑1 (MQ=255)

                                                aCTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCCCGCCGCCGGGTAATAGCAGTGACAACCTCGAGCAACAGATAGCCAGTACTTc                                                                                                      <  2:153059/150‑1 (MQ=255)

                                                aCTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCCCGCAGCCGGGTAATAGCAGTGACAACCTCGAGCAACAGATAGCCAGTACTTc                                                                                                      >  1:2077735/1‑150 (MQ=255)

                                                aCTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCCCGCAGCCGGGTAATAGCAGTGACAACCTCGAGCAACAGATAGCCAGTACTTc                                                                                                      <  2:1483568/150‑1 (MQ=255)

                                                        aGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCCCGCCGCCGGGTAATAGCAGTGACAACCTCGAGCAACAGATAGCCAGTACTTCACAGCaaa                                                                                              >  2:3801125/1‑150 (MQ=255)

                                                          tttCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCCCGCAGCCGGGTAATAGCAGTGACAACCTCGAGCAACAGATAGCCAGTACTTCACAGCAAATc                                                                                            <  2:3554931/150‑1 (MQ=255)

                                                            tCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCCCGCCGCCGGGTAATAGCAGTGACAACCTCGAGCAACAGATAGCCAGTACTTCACAGCAAATCgg                                                                                          >  2:3022144/1‑150 (MQ=255)

                                                             cGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCCCGCCGCCGGGTAATAGCAGTGACAACCTCGAGCAACAGATAGCCAGTACTTCACAGCAAATCggg                                                                                         >  1:3381189/1‑150 (MQ=255)

                                                                aCGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCCCGCCGCCGGGTAATAGCAGTGACAACCTCGAGCAACAGATAGCCAGTACTTCACAGCAAATCGGGtct                                                                                      >  2:3335138/1‑150 (MQ=255)

                                                                  gTTTGCTCGAGGTTTTGATACGGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACCAGTTAGTGAAAAAAAATTAACCCCGCCGCCGGGTAATAGCAGTGACAACCTCGAGCAACAGATAGCCAGTACTTCACAGCAAATCGGGTctct                                                                                    <  2:2666510/150‑1 (MQ=255)

                                                                   tttGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCCCGCCGCCGGGTAATAGCAGTGACAACCTCGAGCAACAGATAGCCAGTACTTCACAGCAAATCGGGTCTCTg                                                                                   <  1:5047106/150‑1 (MQ=255)

                                                                     tGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCCCGCCGCCGGGTAATAGCAGTGACAACCTCGAGCAACAGATAGCCAGTACTTCACAGCAAATCGGGTCTCTGCt                                                                                 >  1:960151/1‑150 (MQ=255)

                                                                      gCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCCCGCCGCCGGGTAATAGCAGTGACAACCTCGAGCAACAGATAGCCAGTACTTCACAGCAAATCGGGTCTCTGCTc                                                                                <  1:595618/150‑1 (MQ=255)

                                                                         cGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCCCGCCGCCGGGTAATAGCAGTGACAACCTCGAGCAACAGATAGCCAGTACTTCACAGCAAATCGGGTCTCTGCTCGcc                                                                             >  2:2859728/1‑150 (MQ=255)

                                                                          gAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCCCGCCGCCGGGTAATAGCAGTGACAACCTCGAGCAACAGATAGCCAGTACTTCACAGCAAATCGGGTCTCTGCTCGCCg                                                                            >  2:2408211/1‑150 (MQ=255)

                                                                            ggTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCCCGCCGCCGGGTAATAGCAGTGACAACCTCGAGCAACAGATAGCCAGTACTTCACAGCAAATCGGGTCTCTGCTCGCCGaa                                                                          >  1:5132552/1‑150 (MQ=255)

                                                                                 tgcgaaTGTCCGCCAGGGTGATGACCTGGAGGTCCCGGCACCCGTTAGTGAAAAAAAATTACCCCCGCCGCCGGGTAATAGCAGTGACAACCTCGAGCAACAGATAGCCAGTACTTCACAGCAAATCGGGTCTCTGCTCGCCGAAGatat                                                                     <  2:4740095/146‑1 (MQ=255)

                                                                                 tGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCCCGCAGCCGGGTAATAGCAGTGACAACCTCGAGCAACAGATAGCCAGTACTTCACAGCAAATCGGGTCTCTGCTCGCCGAAGatat                                                                     >  2:4150336/1‑150 (MQ=255)

                                                                                     aaTGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCCCGCCGCCGGGTAATAGCAGTGACAACCTCGAGCAACAGATAGCCAGTACTTCACAGCAAATCGGGTCTCTGCTCGCCGAAGATATGAAc                                                                 >  2:1781367/1‑150 (MQ=255)

                                                                                       tGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCCCGCCGCCGGGTAATAGCAGTGACAACCTCGAGCAACAGATAGCCAGTACTTCACAGCAAATCGGGTCTCTGCTCGCCGAAGATATGAACAg                                                               >  2:200116/1‑150 (MQ=255)

                                                                                        gTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCCCGCCGCCGGGTAATAGCAGTGACAACCTCGAGCAACAGATAGCCAGTACTTCACAGCAAATCGGGTCTCTGCTCGCCGAAGATATGAACAGc                                                              <  2:1677759/150‑1 (MQ=255)

                                                                                          ccGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCCCGCCGCCGGGTAATAGCAGTGACAACCTCGAGCAACAGATAGCCAGTACTTCACAGCAAATCGGGTCTCTGCTCGCCGAAGATATGAACAGCGa                                                            <  2:4012339/150‑1 (MQ=255)

                                                                                             ccAGTGTGATGAACTGGATGTACCGGCACAAGTTAGTGAAAAAAAATTAACCCCGCCGCCGGGTAATAGCAGTGACAACCTCGAGCAACAGATAGCCAGTACTTCACAGCAAATCGGGTCTCTGCTCGCCGAAGATATGAACAGCGAGCa                                                         <  2:747942/150‑1 (MQ=255)

                                                                                              cAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCCCGCCGCCGGGTAATAGCAGTGACAACCTCGAGCAACAGATAGCCAGTACTTCACAGCAAATCGGGTCTCTGCTCGCCGAAGATATGAACAGCGAGCac                                                        >  2:893586/1‑149 (MQ=255)

                                                                                              cAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCCCGCCGCCGGGTAATAGCAGTGACAACCTCGAGCAACAGATAGCCAGTACTTCACAGCAAATCGGGTCTCTGCTCGCCGAAGATATGAACAGCGAGCaa                                                        <  1:3292368/150‑1 (MQ=255)

                                                                                              cAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCCCGCCGCCGGGTAATAGCAGTGACAACCTCGAGCAACAGATAGCCAGTACTTCACAGCAAATCGGGTCTCTGCTCGCCGAAGATATGAACAGCGAGCaa                                                        <  1:3037111/150‑1 (MQ=255)

                                                                                               aGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCCCGCCGCCGGGTAATAGCAGTGACAACCTCGAGCAACAGATAGCCAGTACTTCACAGCAAATCGGGTCTCTGCTCGCCGAAGATATGAACAGCGAGCAAg                                                       <  2:509056/150‑1 (MQ=255)

                                                                                               aGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCCCGCCGCCGGGTAATAGCAGTGACAACCTCGAGCAACAGATAGCCAGTACTTCACAGCAAATCGGGTCTCTGCTCGCCGAAGATATGAACAGCGAGCAAg                                                       >  2:984041/1‑150 (MQ=255)

                                                                                               aGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCCCGCCGCCGGGTAATAGCAGTGACAACCTCGAGCAACAGATAGCCAGTACTTCACAGCAAATCGGGTCTCTGCTCGCCGAAGATATGAACAGCGAGCAAg                                                       >  2:2445772/1‑150 (MQ=255)

                                                                                                 ggTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCCCGCCGCCGGGTAATAGCAGTGACAACCTCGAGCAACAGATAGCCAGTACTTCACAGCAAATCGGGTCTCTGCTCGCCGAAGATATGAACAGCGAGCAAGCg                                                     >  1:1463794/1‑150 (MQ=255)

                                                                                                  gTGATGAACTGGATGTACCGGCACAAGTTAGTGAAAAAAAATTAACCCCGCCGCCGGGTAATAGCAGTGACAACCTCGAGCAACAGATAGCCAGTACTTCACAGCAAATCGGGTCTCTGCTCGCCGAAGATATGAACAGCGAGCAAGCgg                                                    <  1:1947207/150‑1 (MQ=255)

                                                                                                     atgaACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCCCGCCGCCGGGTAATAGCAGTGACAACCTCGAGCAACAGATAGCCAGTACTTCACAGCAAATCGGGTCTCTGCTCGCCGAAGATATGAACAGCGAGCAAGCGGCaa                                                 >  1:2655646/1‑150 (MQ=255)

                                                                                                           tGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCCCGCCGCCGGGTAATAGCAGTGACAACCTCGAGCAACAGATAGCCAGTACTTCACAGCAAATCGGGTCTCTGCTCGCCGAAGATATGAACAGCGAGCAAGCGGCAAATATgg                                           >  1:2623157/1‑150 (MQ=255)

                                                                                                           tGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCCCGCCGCCGGGTAATAGCAGTGACAACCTCGAGCAACAGATAGCCAGTACTTCACAGCAAATCGGGTCTCTGCTCGCCGAAGATATGAACAGCGAGCAAGCGGCAAATATgg                                           >  1:983851/1‑150 (MQ=255)

                                                                                                                 tCCCGGCACAAGTTAGTGAAAAAAAATTAACCCCGCCGCCGGGTAATAGCAGTGACAACCTCGAGCAACAGATAGCCAGTACTTCACAGCAAATCGGGTCTCTGCTCGCCGAAGATATGAACAGCGAGCAAGCGGCAAATATGGCGCGTg                                     >  1:2814483/1‑150 (MQ=255)

                                                                                                                  cccGGCACAAGTTAGTGAAAAAAAATTAACCCCGCCGCCGGGTAATAGCAGTGACAACCTCGAGCAACAGATAGCCAGTACTTCACAGCAAATCGGGTCTCTGCTCGCCGAAGATATGAACAGCGAGCAAGCGGCAAATATGGCGCGTgg                                    <  1:1455735/150‑1 (MQ=255)

                                                                                                                  cccGGCACAAGTTAGTGAAAAAAAATTAACCCCGCCGCCGGGTAATAGCAGTGACAACCTCGAGCAACAGATAGCCAGTACTTCACAGCAAATCGGGTCTCTGCTCGCCGAAGATATGAACAGCGAGCAAGCGGCAAATATGGCGCGTgg                                    >  1:2394993/1‑150 (MQ=255)

                                                                                                                  cccGGCACAAGTTAGTGAAAAAAAATTAACCCCGCCGCCGGGTAATAGCAGTGACAACCTCGAGCAACAGATAGCCAGTACTTCACAGCAAATCGGGTCTCTGCTCGCCGAAGATATGAACAGCGAGCAAGCGGCAAATATGGCGCGTgg                                    <  1:3908706/150‑1 (MQ=255)

                                                                                                                    cGGCACAAGTTAGTGAAAAAAAATTAACCCCGCAGCCGGGTAATAGCAGTGACAACCTCGAGCAACAGATAGCCAGTACTTCACAGCAAATCGGGTCTCTGCTCGCCGAAGATATGAACAGCGAGCAAGCGGCAAATATGGCGCGTGGAt                                  <  2:3276621/150‑1 (MQ=255)

                                                                                                                      gCACAAGTTAGTGAAAAAAAATTAACCCCGCCGCCGGGTAATAGCAGTGACAACCTCGAGCAACAGATAGCCAGTACTTCACAGCAAATCGGGTCTCTGCTCGCCGAAGATATGAACAGCGAGCAAGCGGCAAATATGGCGCGTGGATgg                                >  1:1032118/1‑150 (MQ=255)

                                                                                                                        acaAGTTAGTGAAAAAAAATTAACCCCGCCGCCGGGTAATAGCAGTGACAACCTCGAGCAACAGATAGCCAGTACTTCACAGCAAATCGGGTCTCTGCTCGCCGAAGATATGAACAGCGAGCAAGCGGCAAATATGGCGCGTGGATGGGc                              >  1:3987347/1‑150 (MQ=255)

                                                                                                                         caAGTTAGTGAAAAAAAATTAACCCCGCCGCCGGGTAATAGCAGTGACAACCTCGAGCAACAGATAGCCAGTACTTCACAGCAAATCGGGTCTCTGCTCGCCGAAGATATGAACAGCGAGCAAGCGGCAAATATGGCGCGTGGATGGGcc                             <  2:4257342/150‑1 (MQ=255)

                                                                                                                         caAGTTAGTGAAAAAAAATTAACCCCGCCGCCGGGTAATAGCAGTGACAACCTCGAGCAACAGATAGCCAGTACTCCACAGCAAATCGGGTCTCTGCTCGCCGAAGATATGAACAGCGAGCAAGCGGCAAATATGGCGCGTGGATGGGcc                             >  1:3935861/1‑150 (MQ=255)

                                                                                                                          aaGTTAGTGAAAAAAAATTAACCCCGCCGCCGGGTAATAGCAGTGACAACCTCGAGCAACAGATAGCCAGTACTTCACAGCAAATCGGGTCTCTGCTCGCCGAAGATATGAACAGCGAGCAAGCGGCAAATATGGCGCGTGGATGGGCct                            <  1:4224782/150‑1 (MQ=255)

                                                                                                                          aaGTTAGTGAAAAAAAATTAACCCCGCCGCCGGGTAATAGCAGTGACAACCTCGAGCAACAGATAGCCAGTACTTCACAGCAAATCGGGTCTCTGCTCGCCGAAGATATGAACAGCGAGCAAGCGGCAAATATGGCGCGTGGATGGGCct                            >  2:2106545/1‑150 (MQ=255)

                                                                                                                          aaGTTAGTGAAAAAAAATTAACCCCGCCGCCGGGTAATAGCAGTGACAACCTCGAGCAACAGATAGCCAGTACTTCACAGCAAATCGGGTCTCTGCTCGCCGAAGATATGAACAGCGAGCAAGCGGCAAATATGGCGCGTGGATGGGCct                            >  2:4466569/1‑150 (MQ=255)

                                                                                                                            gTTAGTGAAAAAAAATTAACCCCGCCGCCGGGTAATAGCAGTGACAACCTCGAGCAACAGATAGCCAGTACTTCACAGCAAATCGGGTCTCTGCTCGCCGAAGATATGAACAGCGAGCAAGCGGCAAATATGGCGCGTGGATGGGCctct                          <  2:1499613/150‑1 (MQ=255)

                                                                                                                             ttAGTGAAAAAAAATTAACCCCGCCGCCGGGTAATAGCAGTGACAACCTCGAGCAACAGATAGCCAGTACTTCACAGCAAATCGGGTCTCTGCTCGCCGAAGATATGAACAGCGAGCAAGCGGCAAATATGGCGCGTGGATGGGCCtctt                         <  2:4417467/150‑1 (MQ=255)

                                                                                                                              tAGTGAAAAAAAATTAACCCCGCCGCCGGGTAATAGCAGTGACAACCTCGAGCAACAGATAGCCAGTACTTCACAGCAAATCGGGTCTCTGCTCGCCGAAGATATGAACAGCGAGCAAGCGGCAAATATGGCGCGTGGATGGGCCtcttc                        >  1:4369111/1‑150 (MQ=255)

                                                                                                                              tAGTGAAAAAAAATTAACCCCGCCGCCGGGTAATAGCAGTGACAACCTCGAGCAACAGATAGCCAGTACTTCACAGCAAATCGGGTCTCTGCTCGCCGAAGATATGAACAGCGAGCAAGCGGCAAATATGGCGCGTGGATGGGCCtcttc                        <  2:2199867/150‑1 (MQ=255)

                                                                                                                              tAGTGAAAAAAAATTAACCCCGCCGCCGGGTAATAGCAGTGACAACCTCGAGCAACAGATAGCCAGTACTTCACAGCAAATCGGGTCTCTGCTCGCCGAAGATATGAACAGCGAGCAAGCGGCAAATATGGCGCGTGGATGGGCCtcttc                        <  1:112868/150‑1 (MQ=255)

                                                                                                                               aGTGAAAAAAAATTAACCCCGCCGCCGGGTAATAGCAGTGACAACCTCGAGCAACAGATAGCCAGTACTTCACAGCAAATCGGGTCTCTGCTCGCCGAAGATATGAACAGCGAGCAAGCGGCAAATATGGCGCGTGGATGGGCCtcttct                       <  2:3487874/150‑1 (MQ=255)

                                                                                                                               aGTGAAAAAAAATTAACCCCGCCGCCGGGTAATAGCAGTGACAACCTCGAGCAACAGATAGCCAGTACTTCACAGCAAATCGGGTCTCTGCTCGCCGAAGATATGAACAGCGAGCAAGCGGCAAATATGGCGCGTGGATGGGCCtcttct                       >  2:4450715/1‑150 (MQ=255)

                                                                                                                                   aaaaaaaaTTAACCCCGCCGCCGGGTAATAGCAGTGACAACCTCGAGCAACAGATAGCCAGTACTTCACAGCAAATCGGGTCTCTGCTCGCCGAAGATATGAACAGCGAGCAAGCGGCAAATATGGCGCGTGGATGGGCCTCTTCTCAgg                   >  2:3814783/1‑150 (MQ=255)

                                                                                                                                    aaaaaaaTTAACCCCGCCGCCGGGTAATAGCAGTGACAACCTCGAGCAACAGATAGCCAGTACTTCACAGCAAATCGGGTCTCTGCTCGCCGAAGATATGAACAGCGAGCAAGCGGCAAATATGGCGCGTGGATGGGCCTCTTCTCAGGc                  <  2:3151001/150‑1 (MQ=255)

                                                                                                                                    aaaaaaaTTAACCCCGCCGCCGGGTAATAGCAGTGACAACCTCGAGCAACAGATAGCCAGTACTTCACAGCAAATCGGGTCTCTGCTCGCCGAAGATATGAACAGCGAGCAAGCGGCAAATATGGCGCGTGGATGGGCCTCTTCTCAGGc                  >  2:1306578/1‑150 (MQ=255)

                                                                                                                                    aaaaaaaTTAACCCCGCCGCCGGGTAATAGCAGTGACAACCTCGAGCAACAGATAGCCAGTACTTCACAGCAAATCGGGTCTCTGCTCGCCGAAGATATGAACAGCGAGCAAGCGGCAAATATGGCGCGTGGATGGGCCTCTTCTCAGGc                  >  2:2234782/1‑150 (MQ=255)

                                                                                                                                       aaaaTTAACCCCGCCGCCGGGTAATAGCAGTGACAACCTCGAGCAACAGATAGCCAGTACTTCACAGCAAATCGGGTCTCTGCTCGCCGAAGATATGAACAGCGAGCAAGCGGCAAATATGGCGCGTGGATGGGCCTCTTCTCAGGCTTc               <  2:1941620/150‑1 (MQ=255)

                                                                                                                                           ttAACCCCGCCGCCGGGTAATAGCAGTGACAACCTCGAGCAACAGATAGCCAGTACTTCACAGCAAATCGGGTCTCTGCTCGCCGAAGATATGAACAGCGAGCAAGCGGCAAATATGGCGCGTGGATGGGCCTCTTCTCAGGCTTCAGgc           >  1:2326783/1‑150 (MQ=255)

                                                                                                                                                cccGCCGCCGGGTAATAGCAGTGACAACCTCGAGCAACAGATAGCCAGTACTTCACAGCAAATCGGGTCTCTGCTCGCCGAAGATATGAACAGCGAGCAAGCGGCAAATATGGCGCGTGGATGGGCCTCTTCTCAGGCTTCAGGCGCAAt      <  2:2381785/150‑1 (MQ=255)

                                                                                                                                                 ccgcagccgGGTAATAGCAGTGACAACCTCGAGCAACAGATAGCCAGTACTTCACAGCAAATCGGGTCTCTGCTCGCCGAAGATATGAACAGCGAGCAAGCGGCAAATATGGCGCGTGGATGGGCCTCTTCTCAGGCTTCAGGCGCAATg     <  1:802370/150‑1 (MQ=255)

                                                                                                                                                  cgccgccgGGTAATAGCAGTGACAACCTCGAGCAACAGATAGCCAGTACTTCACAGCAAATCGGGTCTCTGCTCGCCGAAGATATGAACAGCGAGCAAGCGGCAAATATGGCGCGTGGATGGGCCTCTTCTCAGGCTTCAGGCGCAATGa    >  1:2687773/1‑150 (MQ=255)

CCAAAGCGTTGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCCCGCCGCCGGGTAATAGCAGTGACAACCTCGAGCAACAGATAGCCAGTACTTCACAGCAAATCGGGTCTCTGCTCGCCGAAGATATGAACAGCGAGCAAGCGGCAAATATGGCGCGTGGATGGGCCTCTTCTCAGGCTTCAGGCGCAATGACA  >  CP009273/2038655‑2038952

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`