Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,102,205 C→A 15.1% V287V (GTG→GTT)  wzxB ← putative polisoprenol‑linked O‑antigen transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,102,2050CA15.1% 721.1 / 78.9 233V287V (GTG→GTT) wzxBputative polisoprenol‑linked O‑antigen transporter
Reads supporting (aligned to +/- strand):  ref base C (120/77);  new base A (19/17);  total (139/94)
Fisher's exact test for biased strand distribution p-value = 3.63e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 242 total aligned reads displayed.

AGAGGCGATATAATCATTAGCACAATCACTGCATTATCATATCCCGGCCCTATACTTATTTTTACTAGTATAGATGCACCCAAGAGCAGAATTAATGAAAAAGCACCACCAATCAAACTCAAGCAGGTCAATGATTTTTTAATTAAAATCACACCCTTCACACGATTAAGAACAAGCGTACTTGATATTCTTGGGTATATTGCTTGGGTGATAGGATTTAATAGCCCTTGAAGCGCGTTTCTTATAGTATTGGCCGCATTAAAATTCCCTACGGACGTTGGTCCAGATATAAATCCCAG  >  CP009273/2102056‑2102354
                                                                                                                                                     |                                                                                                                                                     
agagGCGATATAATCATTAGCACAATCACTGCATTATCATATCCCGGCCCTATACTTATTTTTACTAGTATAGATGCACCCGAGAGCAGAATTAATGAAAAAGCACCACCAATCAAACTCAAGCAGGTCAATGATTTTTTAATTAAAATc                                                                                                                                                       >  1:4430362/1‑150 (MQ=255)
   ggCGATATAATCATTAGCACAATCACTGCATTATCATATCCCGGCCCTATACTTATTTTTACTAGTATAGATGCACCCAAGAGCAGAATTAATGAAAAAGCACCACCAATCAAACTCAAGCAGGTCAATGATTTTTTAATTAAAATcaca                                                                                                                                                    >  2:3205794/1‑150 (MQ=255)
     cGATATAATCATTAGCACAATCACTGCATTATCATATCCCGGCCCTATACTTATTTTTACTAGTATAGATGCACCCAAGAGCAGAATTAATGAAAAAGCACCACCAATCAAACTCAAGCAGGTCAATGATTTTTTAATTAAAATCACAcc                                                                                                                                                  >  2:2060823/1‑150 (MQ=255)
       atatAATCATTAGCACAATCACTGCATTATCATATCCCGGCCCTATACTTATTTTTACTAGTATAGATGCACCCAAGAGCAGAATTAATGAAAAAGCACCACCAATCAAACTCAAGCAGGTCAATGATTTTTTAATTAAAATCACACCCt                                                                                                                                                >  2:1081317/1‑150 (MQ=255)
       atatAATCATTAGCACAATCACTGCATTATCATATCCCGGCCCTATACTTATTTTTACTAGTATAGATGCACCCAAGAGCAGAATTAATGAAAAAGCACCACCAATCAAACTCAAGCAGGTCAATGATTTTTTAATTAAAATCACACCCt                                                                                                                                                <  1:194307/150‑1 (MQ=255)
         ataATCATTAGCACAATCACTGCATTATCATATCCCGGCCCTATACTTATTTTTACTAGTATAGATGCACCCAAGAGCAGAATTAATGAAAAAGCACCACCAATCAAACTCAAGCAGGTCAATGATTTTTTAATTAAAATCACACCCTTc                                                                                                                                              >  1:3049341/1‑150 (MQ=255)
         ataATCATTAGCACAATCACTGCATTATCATATCCCGGCCCTATACTTATTTTTACTAGTATAGATGCACCCAAGAGCAGAATTAATGAAAAAGCACCACCAATCAAACTCAAGCAGGTCAATGATTTTTTAATTAAAATAACACCCTTc                                                                                                                                              >  2:4184816/1‑150 (MQ=255)
          taATCATTAGCACAATCACTGCATTATCATATCCCGGCCCTATACTTATTTTTACTAGTATAGATGCACCCAAGAGCAGAATTAATGAAAAAGCACCACCAATCAAACTCAAGCAGGTCAATGATTTTTTAATTAAAATCACACCCTTca                                                                                                                                             <  2:654633/150‑1 (MQ=255)
           aaTCATTAGCACAATCACTGCATTATCATATCCCGGCCCTATACTTATTTTTACTAGTATAGATGCACCCAAGAGCAGAATTAATGAAAAAGCACCACCAATCAAACTCAAGCAGGTCAATGATTTTTTAATTAAAATAACACCCTTcac                                                                                                                                            <  2:3408533/150‑1 (MQ=255)
           aaTCATTAGCACAATCACTGCAGTATCATATCCCGGCCCTATACTTATTTTTACTAGTATAGATGCACCCAAGAGCAGAATTAATGAAAAAGCACCACCAATCAAACTCAAGCAGGTCAATGATTTTTTAATTAAAATAACACCCTTcac                                                                                                                                            <  2:3112659/150‑1 (MQ=255)
            aTCATTAGCACAATCACTGCATTATCATATCCCGGCCCTATACTTATTTTTACTAGTATAGATGCACCCAAGAGCAGAATTAATGAAAAAGCACCACCAATCAAACTCAAGCAGGTCAATGATTTTTTAATTAAAATCACACCCTTcaca                                                                                                                                           >  2:2433543/1‑150 (MQ=255)
             tCATTAGCACAATCACTGCATTATCATATCCCGGCCCTATACTTATTTTTACTAGTATAGATGCACCCAAGAGCAGAATTAATGAAAAAGCACCACCAATCAAACTCAAGCAGGTCAATGATTTTTTAATTAAAATCACACCCTTCacac                                                                                                                                          >  2:4297330/1‑150 (MQ=255)
             tCATTAGCACAATCACTGCATTATCATATCCCGGCCCTATACTTATTTTTACTAGTATAGATGCACCCAAGAGCAGAATTAATGAAAAAGCACCACCAATCAAACTCAAGCAGGTCAATGATTTTTTAATTAAAATCACACCCTTCacac                                                                                                                                          <  1:2241044/150‑1 (MQ=255)
              cATTAGCACAATCACTGCATTATCATATCCCGGCCCTATACTTATTTTTACTAGTATAGATGCACCCAAGAGCAGAATTAATGAAAAAGCACCACCAATCAAACTCAAGCAGGTCAATGATTTTTTAATTAAAATCACACCCTTCACACg                                                                                                                                         >  1:2920270/1‑150 (MQ=255)
               aTTAGCACAATCACTGCATTATCATATCCCGGCCCTATACTTATTTTTACTAGTATAGATGCACCCAAGAGCAGAATTAATGAAAAAGCACCACCAATCAAACTCAAGCAGGTCAATGATTTTTTAATTAAAATCACACCCTTCACACGa                                                                                                                                        >  2:4155573/1‑150 (MQ=255)
               aTTAGCACAATCACTGCATTATCATATCCCGGCCCTATACTTATTTTTACTAGTATAGATGCACCCAAGAGCAGAATTAATGAAAAAGCACCACCAATCAAACTCAAGCAGGTCAATGATTTTTTAATTAAAATCACACCCTTCACACGa                                                                                                                                        >  1:508934/1‑150 (MQ=255)
                ttAGCACAATCACTGCATTATCATATCCCGGCCCTATACTTATTTTTACTAGTATAGATGCACCCAAGAGCAGAATTAATGAAAAAGCACCACCAATCAAACTCAAGCAGGTCAATGATTTTTTAATTAAAATCACACCCTTCACACGAt                                                                                                                                       <  2:834430/150‑1 (MQ=255)
                   gCACCATCACTGCATGATCAGATCCCGGCCCTATACTTATTTTTACTAGTATAGATGCACCCAAGAGCAGAATTAATGAAAAAGCACCACCAATCAAACTCAAGCAGGTCAATGATTTTTTAATTAAAATCACACCCTTCACACGATTaa                                                                                                                                    <  2:502508/150‑1 (MQ=255)
                      caATCACTGCATTATCATATCCCGGCCCTATACTTATTTTTACTAGTATAGATGCACCCAAGAGCAGAATTAATGAAAAAGCACCACCAATCAAACTCAAGCAGGTCAATGATTTTTTAATTAAAATCACACCCTTCACACGATTAAGaa                                                                                                                                 >  1:636118/1‑150 (MQ=255)
                          cACTGCATTATCATATCCCGGCCCTATACTTATTTTTACTAGTATAGATGCACCCAAGAGCAGAATTAATGAAAAAGCACCACCAATCAAACTCAAGCAGGTCAATGATTTTTTAATTAAAATCACACCCTTCACACGATTAAGAACAAg                                                                                                                             >  2:2642551/1‑150 (MQ=255)
                           aCTGCATTATCATATCCCGGCCCTATACTTATTTTTACTAGTATAGATGCACCCAAGAGCAGAATTAATGAAAAAGCACCACCAATCAAACTCAAGCAGGTCAATGATTTTTTAATTAAAATCACACCCTTCACACGATTAAGAACAAGc                                                                                                                            >  2:1623860/1‑150 (MQ=255)
                           aCTGCATTATCATATCCCGGCCCTATACTTATTTTTACTAGTATAGATGCACCCAAGAGCAGAATTAATGAAAAAGCACCACCAATCAAACTCAAGCAGGTCAATGATTTTTTAATTAAAATCACACCCTTCACACGATTAAGAACAAGc                                                                                                                            <  1:1174958/150‑1 (MQ=255)
                           aCTGCATTATCATATCCCGGCCCTATACTTATTTTTACTAGTATAGATGCACCCAAGAGCAGAATTAATGAAAAAGCACCACCAATCAAACTCAAGCAGGTCAATGATTTTTTAATTAAAATCACACCCTTCACACGATTAAGAACAAGc                                                                                                                            >  1:2031041/1‑150 (MQ=255)
                             tGCATTATCATATCCCGGCCCTATACTTATTTTTACTAGTATAGATGCACCCAAGAGCAGAATTAATGAAAAAGCACCACCAATCAAACTCAAGCAGGTCAATGATTTTTTAATTAAAATCACACCCTTCACACGATTAAGAACAAGCGt                                                                                                                          >  1:1845179/1‑150 (MQ=255)
                                 ttATCATATCCCGGCCCTATACTTATTTTTACTAGTATAGATGCACCCAAGAGCAGAATTAATGAAAAAGCACCACCAATCAAACTCAAGCAGGTCAATGATTTTTTAATTAAAATCACACCCTTCACACGATTAAGAACAAGCGTACtt                                                                                                                      >  1:4647283/1‑150 (MQ=255)
                                 ttATCATATCCCGGCCCTATACTTATTTTTACTAGTATAGATGCACCCAAGAGCAGAATTAATGAAAAAGCACCACCAATCAAACTCAAGCAGGTCAATGATTTTTTAATTAAAATCACACCCTTCACACGATTAAGAACAAGCGTACtt                                                                                                                      <  2:2322402/150‑1 (MQ=255)
                                  tATCATATCCCGGCCCTATACTTATTTTTACTAGTATAGATGCACCCAAGAGCAGAATTAATGAAAAAGCACCACCAATCAAACTCAAGCAGGTCAATGATTTTTTAATTAAAATAACACCCTTCACACGATTAAGAACAAGCGTACTTg                                                                                                                     <  2:2398957/150‑1 (MQ=255)
                                    tCATATCCCGGCCCTATACTTATTTTTACTAGTATAGATGCACCCAAGAGCAGAATTAATGAAAAAGCACCACCAATCAAACTCAAGCAGGTCAATGATTTTTTAATTAAAATCACACCCTTCACACGATTAAGAACAAGCGTACTTGat                                                                                                                   >  1:4518167/1‑150 (MQ=255)
                                       tatCCCGGCCCTATACTTATTTTTACTAGTATAGATGCACCCAAGAGCAGAATTAATGAAAAAGCACCACCAATCAAACTCAAGCAGGTCAATGATTTTTTAATTAAAATCACACCCTTCACACGATTAAGAACAAGCGTACTTGATAtt                                                                                                                >  2:2594061/1‑150 (MQ=255)
                                         tCCCGGCCCTATACTTATTTTTACTAGTATAGATGCACCCAAGAGCAGAATTAATGAAAAAGCACCACCAATCAAACTCAAGCAGGTCAATGATTTTTTAATTAAAATCACACCCTTCACACGATTAAGAACAAGCGTACTTGATATTCt                                                                                                              >  1:3954936/1‑150 (MQ=255)
                                          cccGGCCCTATACTTATTTTTACTAGTATAGATGCACCCAAGAGCAGAATTAATGAAAAAGCACCACCAATCAAACTCAAGCAGGTCAATGATTTTTTAATTAAAATCACACCCTTCACACGATTAAGAACAAGCGTACTTGATATTCtt                                                                                                             >  1:3904413/1‑150 (MQ=255)
                                            cGGCCCTATACTTATTTTTACTAGTATAGATGCACCCAAGAGCAGAATTAATGAAAAAGCACCACCAATCAAACTCAAGCAGGTCAATGATTTTTTAATTAAAATCACACCCTTCACACGATTAAGAACAAGCGTACTTGATATTCTTgg                                                                                                           >  2:4638965/1‑150 (MQ=255)
                                               cccTATACTTATTTTTACTAGTATAGATGCACCCAAGAGCAGAATTAATGAAAAAGCACCACCAATCAAACTCAAGCAGGTCAATGATTTTTTAATTAAAATCACACCCTTCACACGATTAAGAACAAGCGTACTTGATATTCTTGGGta                                                                                                        <  2:3888754/150‑1 (MQ=255)
                                               cccTATACTTATTTTTACTAGTATAGATGCACCCAAGAGCAGAATTAATGAAAAAGCACCACCAATCAAACTCAAGCAGGTCAATGATTTTTTAATTAAAATCACACCCTTCACACGATTAAGAACAAGCGTACTTGATATTCTTGGGta                                                                                                        >  2:2759296/1‑150 (MQ=255)
                                                ccTATACTTATTTTTACTAGTATAGATGCACCCAAGAGCAGAATTAATGAAAAAGCACCACCAATCAAACTCAAGCAGGTCAATGATTTTTTAATTAAAATCACACCCTTCACACGATTAAGAACAAGCGTACTTGATATTCTTGGGtat                                                                                                       >  1:4371502/1‑150 (MQ=255)
                                                  tataCTTATTTTTACTAGTATAGATGCACCCAAGAGCAGAATTAATGAAAAAGCACCACCAATCAAACTCAAGCAGGTCAATGATTTTTTAATTAAAATCACACCCTTCACACGATTAAGAACAAGCGTACTTGATATTCTTGGGTatat                                                                                                     >  1:1213124/1‑150 (MQ=255)
                                                    taCTTATTTTTACTAGTATAGATGCACCCAAGAGCAGAATTAATGAAAAAGCACCACCAATCAAACTCAAGCAGGTCAATGATTTTTTAATTAAAATCACACCCTTCACACGATTAAGAACAAGCGTACTTGATATTCTTGGGTATATTg                                                                                                   <  1:4714629/150‑1 (MQ=255)
                                                      cTTATTTTTACTAGTATAGATGCACCCAAGAGCAGAATTAATGAAAAAGCACCACCAATCAAACTCAAGCAGGTCAATGATTTTTTAATTAAAATCACACCCTTCACACGATTAAGAACAAGCGTACTTGATATTCTTGGGTATATTGCt                                                                                                 <  2:3509508/150‑1 (MQ=255)
                                                       ttATTTTTACTAGTATAGATGCACCCAAGAGCAGAATTAATGAAAAAGCACCACCAATCAAACTCAAGCAGGTCAATGATTTTTTAATTAAAATCACACCCTTCACACGATTAAGAACAAGCGTACTTGATATTCTTGGGTATATTGCtt                                                                                                <  1:3439038/150‑1 (MQ=255)
                                                       ttATTTTTACTAGTATAGATGCACCCAAGAGCAGAATTAATGAAAAAGCACCACCAATCAAACTCAAGCAGGTCAATGATTTTTTAATTAAAATCACACCCTTCACACGATTAAGAACAAGCGTACTTGATATTCTTGGGTATATTGCtt                                                                                                <  2:3593391/150‑1 (MQ=255)
                                                            tttACTAGTATAGATGCACCCAAGAGCAGAATTAATGAAAAAGCACCACCAATCAAACTCAAGCAGGTCAATGATTTTTTAATTAAAATCACACCCTTCAGACGATTAAGAACAAGCGTACTTGATATTCTTGGGTATATTGCTTGGGTg                                                                                           >  2:2927397/1‑150 (MQ=255)
                                                              tACTAGTATAGATGCACCCAAGAGCAGAATTAATGAAAAAGCACCACCAATCAAACTCAAGCAGGTCAATGATTTTTTAATTAAAATCACACCCTTCACACGATTAAGAACAAGCGTACTTGATATTCTTGGGTATATTGCTTGGGTGAt                                                                                         >  2:4965579/1‑150 (MQ=255)
                                                              tACTAGTATAGATGCACCCAAGAGCAGAATTAATGAAAAAGCACCACCAATCAAACTCAAGCAGGTCAATGATTTTTTAATTAAAATCACACCCTTCACACGATTAAGAACAAGCGTACTTGATATTCTTGGGTATATTGCTTGGGTGAt                                                                                         <  2:1393917/150‑1 (MQ=255)
                                                                    tataGATGCACCCAAGAGCAGAATTAATGAAAAAGCACCACCAATCAAACTCAAGCAGGTCAATGATTTTTTAATTAAAATCACACCCTTCACACGATTAAGAACAAGCGTACTTGATATTCTTGGGTATATTGCTTGGGTGATAGGAtt                                                                                   >  1:4279934/1‑150 (MQ=255)
                                                                     ataGATGCACCCAAGAGCAGAATTAATGAAAAAGCACCACCAATCAAACTCAAGCAGGTCAATGATTTTTTAATTAAAATCACACCCTTCACACGATTAAGAACAAGCGTACTTGATATTCTTGGGTATATTGCTTGGGTGATAGGAttt                                                                                  <  1:2758065/150‑1 (MQ=255)
                                                                        gATGCACCCAAGAGCAGAATTAATGAAAAAGCACCACCAATCAAACTCAAGCAGGTCAATGATTTTTTAATTAAAATCACACCCTTCACACGATTAAGAACAAGCGTACTTGATATTCTTGGGTATATTGCTTGGGTGATAGGATTTAAt                                                                               >  2:355750/1‑150 (MQ=255)
                                                                        gATGCACCCAAGAGCAGAATTAATGAAAAAGCACCACCAATCAAACTCAAGCAGGTCAATGATTTATTAATTAAAATCACACCCTTCACACGATTAAGAACAAGCGTACTTGATATTCTTGTGTATATTGCTTGGGTGATAGGATTTAAt                                                                               >  2:355495/1‑150 (MQ=255)
                                                                            cACCCAAGAGCAGAATTAATGAAAAAGCACCACCAATCAAACTCAAGCAGGTCAATGATTTTTTAATTAAAATCACACCCTTCACACGATTAAGAACAAGCGTACTTGATATTCTTGGGTATATTGCTTGGGTGATAGGATTTAATAGcc                                                                           <  2:1960472/150‑1 (MQ=255)
                                                                             aCCCAAGAGCAGAATTAATGAAAAAGCACCACCAATCAAACTCAAGCAGGTCAATGATTTTTTAATTAAAATCACACCCTTCACACGATTAAGAACAAGCGTACTTGATATTCTTGGGTATATTGCTTGGGTGATAGGATTTAATAGccc                                                                          >  1:4972130/1‑150 (MQ=255)
                                                                             aCCCAAGAGCAGAATTAATGAAAAAGCACCACCAATCAAACTCAAGCAGGTCAATGATTTTTTAATTAAAATCACACCCTTCACACGATTAAGAACAAGCGTACTTGATATTATTGGGTATATTGCTTGGGTGATAGGATTTAATAcccc                                                                          <  1:3056346/150‑1 (MQ=255)
                                                                              cccAAGAGCAGAATTAATGAAAAAGCACCACCAATCAAACTCAAGCAGGTCAATGATTTTTTAATTAAAATCACACCCTTCACACGATTAAGAACAAGCGTACTTGATATTCTTGGGTATATTGCTTGGGTGATAGGATTTAATAGCCCt                                                                         <  2:3794120/150‑1 (MQ=255)
                                                                                 aaGAGCAGAATTAATGAAAAAGCACCACCAATCAAACTCAAGCAGGTCAATGATTTTTTAATTAAAATAACACCCTTCACACGATTAAGAACAAGCGTACTTGATATTCTTGGGTATATTGCTTGGGTGATAGGATTTAATAGCCCTTGa                                                                      >  1:967123/1‑150 (MQ=255)
                                                                                    agCAGAATTAATGAAAAAGCACCACCAATCAAACTCAAGCAGGTCAATGATTTTTTAATTAAAATCACACCCTTCACACGATTAAGAACAAGCGTACTTGATATTCTTGGGTATATTGCTTGGGTGATAGGATTTAATAGCCCTTGAAgc                                                                   >  2:3476364/1‑150 (MQ=255)
                                                                                     gCAGAATTAATGAAAAAGCACCACCAATCAAACTCAAGCAGGTCAATGATTTTTTAATTAAAATCACACCCTTCACACGATTAAGAACAAGCGTACTTGATATTCTTGGGTATATTGCTTGGGTGATAGGATTTAATAGCCCTTGAAgcg                                                                  <  1:1882122/150‑1 (MQ=255)
                                                                                     gCAGAATTAATGAAAAAGCACCACCAATCAAACTCAAGCAGGTCAATGATTTTTTAATTAAAATCACACCCTTCACACGATTAAGAACAAGCGTACTTGATATTCTTGGGTATATTGCTTGGGTGATAGGATTTAATAGCCCTTGAAgcg                                                                  <  2:1959384/150‑1 (MQ=255)
                                                                                        gAATTAATGAAAAAGCACCACCAATCAAACTCAAGCAGGTCAATGATTTTTTAATTAAAATAACACCCTTCACACGATTAAGAACAAGCGTACTTGATATTCTTGGGTATATTGCTTGGGTGATAGGATTTAATAGCCCTTGAAGCGCGt                                                               >  1:4781958/1‑150 (MQ=255)
                                                                                        gAATTAATGAAAAAGCACCACCAATCAAACTCAAGCAGGTCAATGATTTTTTAATTAAAATAACACCCTTCACACGATTAAGAACAAGCGTACTTGATATTCTTGGGTATATTGCTTGGGTGATAGGATTTAATAGCCCTTGAAGCGCGt                                                               >  1:3352636/1‑150 (MQ=255)
                                                                                         aaTTAATGAAAAAGCACCACCAATCAAACTCAAGCAGGTCAATGATTTTTTAATTAAAATCACACCCTTCACACGATTAAGAACAAGCGTACTTGATATTCTTGGGTATATTGCTTGGGTGATAGGATTTAATAGCCCTTGAAGCGCGtt                                                              <  1:3265524/150‑1 (MQ=255)
                                                                                          aTTAATGAAAAAGCACCACCAATCAAACTCAAGCAGGTCAATGATTTATTAATTAAAATCACACCCTTCACACGATTAAGAACAAGCGTACTTGATATTCTTGGGTATATTGCTTGGGTGATAGGATTTAATAGCCCTTGAAGCGCGttt                                                             >  2:4680467/1‑150 (MQ=255)
                                                                                           ttAATGAAAAAGCACCACCAATCAAACTCAAGCAGGTCAATGATTTTTTAATTAAAATCACACCCTTCACACGATTAAGAACAAGCGTACTTGATATTCTTGGGTTTATTGCTTGGGTGATAGGATTTAATAGCCCTTGAAGCGCGTTTc                                                            <  2:5060234/150‑1 (MQ=255)
                                                                                           ttAATGAAAAAGCACCACCAATCAAACTCAAGCAGGTCAATGATTTTTTAATTAAAATCACACCCTTCACACGATTAAGAACAAGCGTACTTGATATTCTTGGGTATATTGCTTGGGTGATAGGATTTAATAGCCCTTGAAGCGCGTTTc                                                            <  2:3669321/150‑1 (MQ=255)
                                                                                           ttAATGAAAAAGCACCACCAATCAAACTCAAGCAGGTCAATGATTTTTTAATTAAAATCACACCCTTCACACGATTAAGAACAAGCGTACTTGATATTCTTGGGTATATTGCTTGGGTGATAGGATTTAATAGCCCTTGAAGCGCGTTTc                                                            >  1:951070/1‑150 (MQ=255)
                                                                                               tGAAAAAGCACCACCAATCAAACTCAAGCAGGTCAATGATTTTTTAATTAAAATCACACCCTTCACACGATTAAGAACAAGCGTACTTGATATTCTTGGGTATATTGCTTGGGTGATAGGATTTAATAGCCCTTGAAGCGCGTTTCTtat                                                        <  1:2883702/150‑1 (MQ=255)
                                                                                               tGAAAAAGCACCACCAATCAAACTCAAGCAGGTCAATGATTTTTTAATTAAAATCACACCCTTCACACGATTAAGAACAAGCGTACTTGATATTCTTGGGTATATTGCTTGGGTGATAGGATTTAATAGCCCTTGAAGCGCGTTTCTtat                                                        <  1:3852191/150‑1 (MQ=255)
                                                                                               tGAAAAAGCACCACCAATCAAACTCAAGCAGGTCAATGATTTTTTAATTAAAATAACACCCTTCACACGATTAAGAACAAGCGTACTTGATATTCTTGGGTATATTGCTTGGGTGATAGGATTTAATAGCCCTTGAAGCGCGTTTCTtat                                                        <  2:4603449/150‑1 (MQ=255)
                                                                                                  aaaaGCACCACCAATCAAACTCAAGCAGGTCAATGATTTTTTAATTAAAATCACACCCTTCACACGATTAAGAACAAGCGTACTTGATATTCTTGGGTATATTGCTTGGGTGATAGGATTTAATAGCCCTTGAAGCGCGTTTCTTATAGt                                                     >  1:374775/1‑150 (MQ=255)
                                                                                                     aGCACCACCAATCAAACTCAAGCAGGTCAATGATTTTTTAATTAAAATCACACCCTTCACACGATTAAGAACAAGCGTACTTGATATTCTTGGGTATATTGCTTGGGTGATAGGATTTAATAGCCCTTGAAGCGCGTTTCTTATAGTAtt                                                  <  1:2232881/150‑1 (MQ=255)
                                                                                                      gCACCACCAATCAAACTCAAGCAGGTCAATGATTTTTTAATTAAAATCACACCCTTCACACGATTAAGAACAAGCGTACTTGATATTCTTGGGTATATTGCTTGGGTGATAGGATTTAATAGCCCTTGAAGCGCGTTTCTTATAGTATTg                                                 <  1:4010638/150‑1 (MQ=255)
                                                                                                         ccaccaATCAAACTCAAGCAGGTCAATGATTTTTTAATTAAAATCACACCCTTCACACGATTAAGAACAAGCGTACTTGATATTCTTGGGTATATTGCTTGGGTGATAGGATTTAATAGCCCTTGAAGCGCGTTTCTTATAGTATTGGcc                                              >  1:1383540/1‑150 (MQ=255)
                                                                                                            ccaATCAAACTCAAGCAGGTCAATGATTTTTTAATTAAAATCACACCCTTCACACGATTAAGAACAAGCGTACTTGATATTCTTGGGTATATTGCTTGGGTGATAGGATTTAATATCCCTTGAAGCTCGTTTCTTATAGTATTGGCCGCa                                           >  1:2676487/1‑150 (MQ=255)
                                                                                                              aaTCAAACTCAAGCAGGTCAATGATTTTTTAATTAAAATCACACCCTTCACACGATTAAGAACAAGCGTACTTGATATTCTTGGGTATATTGCTTGGGTGATAGGATTTAATAGCCCTTGAAGCGCGTTTCTTATAGTATTGGCCGCAtt                                         <  2:1312034/150‑1 (MQ=255)
                                                                                                              aaTCAAACTCAAGCAGGTCAATGATTTTTTAATTAAAATCACACCCTTCACACGATTAAGAACAAGCGTACTTGATATTCTTGGGTATATTGCTTGGGTGATAGGATTTAATAGCCCTTGAAGCGCGTTTCTTATAGTATTGGCCGCAtt                                         >  1:1835086/1‑150 (MQ=255)
                                                                                                              aaTCAAACTCAAGCAGGTCAATGATTTTTTAATTAAAATAACACCCTTCACACGATTAAGAACAAGCGTACTTGATATTCTTGGGTATATTGCTTGGGTGATAGGATTTAATAGCCCTTGAAGCGCGTTTCTTATAGTATTGGCCGCAtt                                         >  1:2251647/1‑150 (MQ=255)
                                                                                                                  aaaCTCAAGCAGGTCAATGATTTTTTAATTAAAATCACACCCTTCACACGATTAAGAACAAGCGTACTTGATATTCTTGGGTATATTGCTTGGGTGATAGGATTTAATAGCCCTTGAAGCGCGTTTCTTATAGTATTGGCCGCATTaaaa                                     >  1:1122900/1‑150 (MQ=255)
                                                                                                                  aaaCTCAAGCAGGTCAATGATTTTTTAATTAAAATAACACCCTTCACACGATTAAGAACAAGCGTACTTGATATTCTTGGGTATATTGCTTGGGTGATAGGATTTAATAGCCCTTGAAGCGCGTTTCTTATAGTATTGGCCGCATTaaaa                                     >  1:2178928/1‑150 (MQ=255)
                                                                                                                   aaCTCAAGCAGGTCAATGATTTTTTAATTAAAATCACACCCTTCACACGATTAAGAACAAGCGTACTTGATATTCTTGGGTATATTGCTTGGGTGATAGGATTTAATAGCCCTTGAAGCGCGTTTCTTATAGTATTGGCCGCATTAAAAt                                    >  1:2382349/1‑150 (MQ=255)
                                                                                                                     cTCAAGCAGGTCAATGATTTTTTAATTAAAATCACACCCTTCACACGATTAAGAACAAGCGTACTTGATATTCTTGGGTATATTGCTTGGGTGATAGGATTTAATAGCCCTTGAAGCGCGTTTCTTATAGTATTGGCCGCATTAAAATTc                                  >  1:4619202/1‑150 (MQ=255)
                                                                                                                       cAAGCAGGTCAATGATTTTTTAATTAAAATCACACCCTTCACACGATTAAGAACAAGCGTACTTGATATTCTTGGGTATATTGCTTGGGTGATAGGATTTAATAGCCCTTGAAGCGCGTTTCTTATAGTATTGGCCGCATTAAAATTccc                                <  2:2132645/150‑1 (MQ=255)
                                                                                                                        aaGCAGGTCAATGATTTTTTAATTAAAATCACACCCTTCACACGATTAAGAACAAGCGTACTTGATATTCTTGGGTATATTGCTTGGGTGATAGGATTTAATAGCCCTTGAAGCGCGTTTCTTATAGTATTGGCCGCATTAAAATTCCCt                               >  2:2420835/1‑150 (MQ=255)
                                                                                                                          gCAGGTCAATGATTTTTTAATTAAAATCACACCCTTCACACGATTAAGAACAAGCGTACTTGATATTCTTGGGTATATTGCTTGGGTGATAGGATTTAATAGCCCTTGAAGCGCGTTTCTTATAGTATTGGCCGCATTAAAATTCCCTAc                             >  2:4429082/1‑150 (MQ=255)
                                                                                                                           cAGGTCAATGATTTTTTAATTAAAATCACACCCTTCACACGATTAAGAACAAGCGTACTTGATATTCTTGGGTATATTGCTTGGGTGATAGGATTTAATAGCCCTTGAAGCGCGTTTCTTATAGTATTGGCCGCATTAAAATTCCCTACg                            <  2:2053789/150‑1 (MQ=255)
                                                                                                                              gTCAATGATTTTTTAATTAAAATAACACCCTTCACACGATTAAGAACAAGCGTACTTGATATTCTTGGGTATATTGCTTGGGTGATAGGATTTAATAGCCCTTGAAGCGCGTTTCTTATAGTATTGGCCGCATTAAAATTCCCTACGGAc                         <  2:2706901/150‑1 (MQ=255)
                                                                                                                               tCAATGATTTTTTAATTAAAATCACACCCTTCACACGATTAAGAACAAGCGTACTTGATATTCTTGGGTATATTGCTTGGGTGATAGGATTTAATAGCCCTTGAAGCGCGTTTCTTATAGTATTGTCCGCATTAAAATTCCCTACGGACg                        >  2:1267951/1‑150 (MQ=255)
                                                                                                                                 aaTGATTTTTTAATTAAAATCACACCCTTCACACGATTAAGAACAAGCGTACTTGATATTCTTGGGTATATTGCTTGGGTGATAGGATTTAATAGCCCTTGAAGCGCGTTTCTTATAGTATTGGCCGCATTAAAATTCCCTACGGACGtt                      <  2:3049341/150‑1 (MQ=255)
                                                                                                                                    gATTTTTTAATTAAAATCACACCCTTCACACGATTAAGAACAAGCGTACTTGATATTCTTGGGTATATTGCTTGGGTGATAGGATTTAATAGCCCTTGAAGCGCGTTTCTTATAGTATTGGCCGCATTAAAATTCCCTACGGACGTTGGt                   <  2:262398/150‑1 (MQ=255)
                                                                                                                                    gATTTTTTAATTAAAATCACACCCTTCACACGATTAAGAACAAGCGTACTTGATATTCTTGGGTATATTGCTTGGGTGATAGGATTTAATAGCCCTTGAAGCGCGTTTCTTATAGTATTGGCCGCATTAAAATTCCCTACGGACGTTGGt                   <  2:2858076/150‑1 (MQ=255)
                                                                                                                                      ttttttAATTAAATTCACACCCTTCACACGATTAAGAACAAGCGTACTTGATATTCTTGGGTATATTGCTTGGGTGATAGGATTTAATAGCCCTTGAAGCGCGTTTCTTATAGTATTGGCCGCATTAAAATTCCCTACGGACGTTGGTcc                 >  1:244061/1‑150 (MQ=255)
                                                                                                                                      ttttttAATTAAAATCACACCCTTCACACGATTAAGAACAAGCGTACTTGATATTCTTGGGTATATTGCTTGGGTGATAGGATTTAATAGCCCTTGAAGCGCGTTTCTTATAGTATTGGCCGCATTAAAATTCCCTACGGACGTTGGTcc                 <  1:435610/150‑1 (MQ=255)
                                                                                                                                        ttttAATTAAAATCACACCCTTCACACGATTAAGAACAAGCGTACTTGATATTCTTGGGTATATTGCTTGGGTGATAGGATTTAATAGCCCTTGAAGCGCGTTTCTTATAGTATTGGCCGCATTAAAATTCCCTACGGACGTTGGTCCAg               >  1:1255191/1‑150 (MQ=255)
                                                                                                                                        ttttAATTAAAATAACACCCTACACACGATTAAGAACAAGCGTACTTGATATTCTTGGGTATATTGCTTGGGTGATAGGATTTAATAGCCCTTGAAGCGCGTTTCTTATAGTATTGGCCGCATTAAAATTCCCTACGGACGTTGGTCCAg               <  1:4252891/150‑1 (MQ=255)
                                                                                                                                           taattaaAATCACACCCTTCACACGATTAAGAACAAGCGTACTTGATATTCTTGGGTATATTGCTTGGGTGATAGGATTTAATAGCCCTTGAAGCGCGTTTCTTATAGTATTGGCCGCATTAAAATTCCCTACGGACGTTGGTCCAGata            >  1:3751934/1‑150 (MQ=255)
                                                                                                                                             attaaAATCACACCCTTCACACGATTAAGAACAAGCGTACTTGATATTCTTGGGTATATTGCTTGGGTGATAGGATTTAATAGCCCTTGAAGCGCGTTTCTTATAGTATTGGCCGCATTAAAATTCCCTACGGACGTTGGTCCAGAtata          <  1:3186759/150‑1 (MQ=255)
                                                                                                                                             attaaAATAACACCCTTCACACGATTAAGAACAAGCGTACTTGATATTCTTGGGTATATTGCTTGGGTGATAGGATTTAATAGCCCTTGAAGCGCGTTTCTTATAGTATTGGCCGCATTAAAATTCCCTACGGACGTTGGTCCAGAtata          <  2:4016936/150‑1 (MQ=255)
                                                                                                                                               taaAATCACACCCTTCACACGATTAAGAACAAGCGTACTTGATATTCTTGGGTATATTGCTTGGGTGATAGGATTTAATAGCCCTTGAAGCGCGTTTCTTATAGTATTGGCCGCATTAAAATTCCCTACGGACGTTGGTCCAGATATaaa        >  1:4167825/1‑150 (MQ=255)
                                                                                                                                                aaaaTCACACCCTTCACACGATTAAGAACAAGCGTACTTGATATTCTTGGGTATATTGCTTGGGTGATAGGATTTAATAGCCCTTGAAGCGCGTTTCTTATAGTATTGGCCGCATTAAAATTCCCTACGGACGTTGGTCCAGATATAAAt       <  1:3437942/150‑1 (MQ=255)
                                                                                                                                                 aaaTCACACCCTTCACACGATTAAGAACAAGCGTACTTGATATTCTTGGGTATATTGCTTGGGTGATAGGATTTAATAGCCCTTGAAGCGCGTTTCTTATAGTATTGGCCGCATTAAAATTCCCTACGGACGTTGGTCCAGATATAAATc      <  1:4886079/150‑1 (MQ=255)
                                                                                                                                                 aaaTCACACCCTTCACACGATTAAGAACAAGCGTACTTGATATTCTTGGGTATATTGCTTGGGTGATAGGATTTAATAGCCCTTGAAGCGCGTTTCTTATAGTATTGGCCGCATTAAAATTCCCTACGGACGTTGGTCCAGATATAAATc      >  1:3592678/1‑150 (MQ=255)
                                                                                                                                                 aaaTCACACCCTTCACACGATTAAGAACAAGCGTACTTGATATTCTTGGGTATATTGCTTGGGTGATAGGATTTAATAGCCCTTGAAGCGCGTTTCTTATAGTATTGGCCGCATTAAAATTCCCTACGGACGTTGGTCCAGATATAAATc      >  1:3592431/1‑150 (MQ=255)
                                                                                                                                                   aTCACACCCTTCACACGATTAAGAACAAGCGTACTTGATATTCTTGGGTATATTGCTTGGGTGATAGGATTTAATAGCCCTTGAAGCGCGTTTCTTATAGTATTGGCCGCATTAAAATTCCCTACGGACGTTGGTCCAGATATAAATcac    >  2:1778713/1‑148 (MQ=255)
                                                                                                                                                     cacaCCCTTCACACGATTAAGAACAAGCGTACTTGATATTCTTGGGTATATTGCTTGGGTGATAGGATTTAATAGCCCTTGAAGCGCGTTTCTTATAGTATTGGCCGCATTAAAATTCCCTACGGACGTTGGTCCAGATATAAATCCCAg  >  2:57926/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
AGAGGCGATATAATCATTAGCACAATCACTGCATTATCATATCCCGGCCCTATACTTATTTTTACTAGTATAGATGCACCCAAGAGCAGAATTAATGAAAAAGCACCACCAATCAAACTCAAGCAGGTCAATGATTTTTTAATTAAAATCACACCCTTCACACGATTAAGAACAAGCGTACTTGATATTCTTGGGTATATTGCTTGGGTGATAGGATTTAATAGCCCTTGAAGCGCGTTTCTTATAGTATTGGCCGCATTAAAATTCCCTACGGACGTTGGTCCAGATATAAATCCCAG  >  CP009273/2102056‑2102354

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑