Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,115,361 C→A 29.9% R367L (CGC→CTC)  cpsG ← phosphomannomutase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,115,3610CA29.9% 698.2 / 294.0 351R367L (CGC→CTC) cpsGphosphomannomutase
Reads supporting (aligned to +/- strand):  ref base C (105/141);  new base A (56/49);  total (161/190)
Fisher's exact test for biased strand distribution p-value = 7.93e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01

Only 100 of 355 total aligned reads displayed.

GTCGGCAAAGGTCATGCTGATGCCATCGGTGCGATCCACCGCCAGCGCCTCACGGCTAAAATGCTGTTCCACGCGGTTAATCGCCTCAACGGGTTGCGCCAGTTTGCTGTTGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGCGTACCAGTTCGCCCAGCGTTTTATCTTTCAGGCACACCAGTTCGGCGACCAGCAGCCACGGGATCATGCCGCTGTCGCAGTAAGCGAAATCACGGAAGTAATGGTGGGCGCTCATTTCGCCACCATAGATGGCGTCTTCCTTGCGCATA  >  CP009273/2115213‑2115510
                                                                                                                                                    |                                                                                                                                                     
gTCGGCAAAGGTCATGCTGATGCCATCGGTGCGATCCACCGCCAGCGCCTCACGGCTAAAATGCTGTTCCACGCGGTTAATCGCCTCAACGGGTTGCGCCAGTTTGCTGTTGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTcgcg                                                                                                                                                      >  1:5128275/1‑150 (MQ=255)
 tCGGCATAGGTCATGCTGATGCCATCGGTGCGATCCACCGCCAGCGCCTCACGGCTAAAATGCTGTTCCACGCGGTTAATCGCCTCAACGGGTTGCGCCAGTTTGCTGTTGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGCGt                                                                                                                                                     <  2:2326846/150‑1 (MQ=255)
  cGGCAAAGGTCTTGCTGATGCCATCGGTGCGATCCACCGCCAGCGCCTCACGGCTAAAATGCTGTTCCACGCGGTTAATCGCCTCAACGGGTTGCGCCAGTTTGCTGTTGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGCGTa                                                                                                                                                    <  1:1348744/150‑1 (MQ=255)
  cGGCAAAGGTCATGCTGATGCCATCGGTGCGATCCACCGCCAGCGCCTCACGGCTAAAATGCTGTTCCACGCGGTTAATCGCCTCAACGGGTTGCGCCAGTTTGCTGTTGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGAGTa                                                                                                                                                    >  1:4448906/1‑150 (MQ=255)
     cAAAGGTCATGCTGATGCCATCGGTGCGATCCACCGCCAGCGCCTCACGGCTAAAATGCTGTTCCACGCGGTTAATCGCCTCAACGGGTTGCGCCAGTTTGCTGTTGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGAGTACCa                                                                                                                                                 <  1:2157679/150‑1 (MQ=255)
      aaaGGTCATGCTGATGCCATCGGTGCGATCCACCGCCAGCGCCTCACGGCTAAAATGCTGTTCCACGCGGTTAATCGCCTCAACGGGTTGCGCCAGTTTGCTGTTGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGCGTACCAg                                                                                                                                                <  1:4702078/150‑1 (MQ=255)
      aaaGGTCATGCTGATGCCATCGGTGCGATCCACCGCCAGCGCCTCACGGCTAAAATGCTGTTCCACGCGGTTAATCGCCTCAACGGGTTGCGCCAGTTTGCTGTTGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGAGTACCAg                                                                                                                                                >  2:1394520/1‑150 (MQ=255)
        aGGTCATGCTGATGCCATCGGTGCGATCCACCGCCAGCGCCTCACGGCTAAAATGCTGTTCCACGCGGTTAATCGCCTCAACGGGTTGCGCCAGTTTGCTGTTGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGCGTACCAGtt                                                                                                                                              <  2:2819428/150‑1 (MQ=255)
            cATGCTGATGCCATCGGTGCGATCCACCGCCAGCGCCTCACGGCTAAAATGCTGTTCCACGCGGTTAATCGCCTCAACGGGTTGCGCCAGTTTGCTGTTGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGCGTACCAGTTCGcc                                                                                                                                          >  1:1465825/1‑150 (MQ=255)
             aTGCTGATGCCATCGGTGCGATCCACCGCCAGCGCCTCACGGCTAAAATGCTGTTCCACGCGGTTAATCGCCTCAACGGGTTGCGCCAGTTTGCTGTTGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGCGTACCAGTTCGccc                                                                                                                                         >  2:4336143/1‑150 (MQ=255)
             aTGCTGATGCCATCGGTGCGATCCACCGCCAGCGCCTCACGGCTAAAATGCTGTTCCACGCGGTTAATCGCCTCAACGGGTTGCGCCAGTTTGCTGTTGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGCGTACCAGTTCGccc                                                                                                                                         <  1:3624613/150‑1 (MQ=255)
                 tGATGCCATCGGTGCGATCCACCGCCAGCGCCTCACGGCTAAAATGCTGTTCCACGCGGTTAATCGCCTCAACGGGTTGCGCCAGTTTGCTGTTGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGCGTACCAGTTCGCCCAGCg                                                                                                                                     <  2:1484107/150‑1 (MQ=255)
                   aTGCCATCGGTGCGATCCACCGCCAGCGCCTCACGGCTAAAATGCTGTTCCACGCGGTTAATCGCCTCAACGGGTTGCGCCAGTTTGCTGTTGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGAGTACCAGTTCGCCCAGCGtt                                                                                                                                   >  1:4676485/1‑150 (MQ=255)
                      ccATCGGTGCGATCCACCGCCAGCGCCTCACGGCTAAAATGCTGTTCCACGCGGTTAATCGCCTCAACGGGTTGCGCCAGTTTGCTGTTGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGAGTACCAGGTCGCCCAGCGTTTTa                                                                                                                                >  1:1809893/1‑150 (MQ=255)
                       cagcGGTGCGATCCACCGCCAGCGCCTCACGGCTAAAATGCTGTTCCACGCGGTTAATCGCCTCAACGGGTTGCGCCAGTTTGCTGTTGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGCGTACCAGTTCGCCCAGCGTTTTAt                                                                                                                               <  1:4548365/147‑1 (MQ=255)
                       cATCGGTGCGATCCACCGCCAGCGCCTCACGGCTAAAATGCTGTTCCACGCGGTTAATCGCCTCAACGGGTTGCGCCAGTTTGCTGTTGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGCGTACCAGTTCGCCCAGCGTTTTAt                                                                                                                               <  1:107776/150‑1 (MQ=255)
                       cATCGGTGCGATCCACCGCCAGCGCCTCACGGCTAAAATGCTGTTCCACGCGGTTAATCGCCTCAACGGGTTGCGCCAGTTTGCTGTTGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGCGTACCAGTTCGCCCAGCGTTTTAt                                                                                                                               <  1:4899230/150‑1 (MQ=255)
                         tCGGTGCGATCCACCGCCAGCGCCTCACGGCTAAAATGCTGTTCCACGCGGTTAATCGCCTCAACGGGTTGCGCCAGTTTGCTGTTGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGCGTACCAGTTCGCCCAGCGTGTTATCt                                                                                                                             >  2:1007159/1‑150 (MQ=255)
                             tGCGATCCACCGCCAGCGCCTCACGGCTAAAATGCTGTTCCACGCGGTTAATCGCCTCAACGGGTTGCGCCAGTTTGCTGTTGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGCGTACCAGTTCGCCCAGCGTTTTATCTTTCa                                                                                                                         <  1:3864528/150‑1 (MQ=255)
                             tGCGATCCACCGCCAGCGCCTCACGGCTAAAATGCTGTTCCACGCGGTTAATCGCCTCAACGGGTTGCGCCAGTTTGCTGTTGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGCGTACCAGTTCGCCCAGCGTTTTATCTTTCa                                                                                                                         >  2:4650462/1‑150 (MQ=255)
                               cGATCCACCGCCAGCGCCTCACGGCTAAAATGCTGTTCCACGCGGTTAATCGCCTCAACGGGTTGCGCCAGTTTGCTGTTGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGAGTACCAGTTCGCCCAGCGGTTTATCTTTCAgg                                                                                                                       >  2:4489166/1‑150 (MQ=255)
                                gATCCACCGCCAGCGCCTCACGGCTAAAATGCTGTTCCACGCGGTTAATCGCCTCAACGGGTTGCGCCAGTTTGCTGTTGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGCGTACCAGTTCGCCCAGCGTTTTATCTTTCAGGc                                                                                                                      >  2:2412703/1‑150 (MQ=255)
                                 aTCCACCGCCAGCGCCTCACGGCTAAAATGCTGTTCCACGCGGTTAATCGCCTCAACGGGTTGCGCCAGTTTGCTGTTGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGCGTACCAGTTCGCCCAGCGTTTTATCTTTCAGGca                                                                                                                     >  1:1642365/1‑150 (MQ=255)
                                 aTCCACCGCCAGCGCCTCACGGCTAAAATGCTGTTCCACGCGGTTAATCGCCTCAACGGGTTGCGCCAGTTTGCTGTTGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGAGTACCAGTTCGCCCAGCGTTTTATCTTTCAGGca                                                                                                                     <  2:4928331/150‑1 (MQ=255)
                                  tCCACCGCCAGCGCCTCACGGCTAAATTGCTGTTCCACGCGGTTATTCGCCTAAACGTGTTGCGCCAGTTTGCTGTTGATCCCACCGCTTGCCGGAAACGACTACATCCGGTCGAGTACCAGTTCGCCCAGCGTTTTATCTTTGAGGcac                                                                                                                    <  1:1182706/150‑1 (MQ=255)
                                  tCCACCGCCAGCGCCTCACGGCTAAAATGCTGTTCCACGCGGTTAATCGCCTCAACGGGTTGCGCCAGTTTGCTGTTGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGAGTACCAGTTCGCCCAGCGTTTTATCTTTCAGGcac                                                                                                                    <  1:2758644/150‑1 (MQ=255)
                                  tCCACCGCCAGCGCCTCACGGCTAAAATGCTGTTCCACGCGGTTAATCGCCTCAACGGGTTGCGCCAGTTTGCTGTTGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGGCGCGTACCAGTGCGCCCAGCGTTGTATCGTTCAGGcac                                                                                                                    >  2:3542976/1‑150 (MQ=255)
                                    cACCGCCAGCGCCTCACGGCTAAAATGCTGTTCCACGCGGTTAATCGCCTCAACGGGTTGCGCCAGTTTGCTGTTGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGCGTACCAGTTCGCCCAGCGTTTTATCTTTCAGGCacac                                                                                                                  >  1:1780391/1‑150 (MQ=255)
                                      ccGCCAGCGCCTCACGGCTAAAATGCTGTTCCACGCGGTTAATCGCCTCAACGGGTTGCGCCAGTTTGCTGTTGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGCGTACCAGTTCGCCCAGCGTTTTATCTTTCAGGCACACCa                                                                                                                <  1:1531226/150‑1 (MQ=255)
                                         ccAGCGCCTCACGGCTAAAATGCTGTTCCACGCGGTTAATCGCCTCAACGGGTTGCGCCAGTTTGCTGTTGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGCGTACCAGTTCGCCCAGCGTTTTATCTTTCAGGCACACCAGtt                                                                                                             <  1:3517544/150‑1 (MQ=255)
                                          cAGCGCCTCACGGCTAAAATGCTGTTCCACGCGGTTAATCGCCTCAACGGGTTGCGCCAGTTTGCTGTTGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGAGTACCAGTTCGCCCAGCGTTTTATCTTTCAGGCACACCAGTTc                                                                                                            >  1:3338570/1‑150 (MQ=255)
                                           aGCGCCTCACGGCTAAAATGCTGTTCCACGCGGTTAATCGCCTCAACGGGTTGCGCCAGTTTGCTGTTGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGCGTACCAGTTCGCCCAGCGTTTTATCTTTCAGGCACACCAGTTCg                                                                                                           <  1:2439150/150‑1 (MQ=255)
                                             cgcCTCACGGCTAAAATGCTGTTCCACGCGGTTAATCGCCTCAACGGGTTGCGCCAGTTTGCTGTTGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGCGTACCAGTTCGCCCAGCGTTTTATCTTTCAGGCACACCAGTTCGGc                                                                                                         <  1:1006585/150‑1 (MQ=255)
                                             cgcCTCACGGCTAAAATGCTGTTCCACGCGGTTAATCGCCTCAACGGGTTGCGCCAGTTTGCTGTTGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGCGTACCAGTTCGCCCAGCGTTTTATCTTTCAGGCACACCAGTTCGGc                                                                                                         >  1:4398926/1‑150 (MQ=255)
                                              gcCTCACGGCTAAAATGCTGTTCCACGCGGTTAATCGCCTCAACGGGTTGCGCCAGTTTGCTGTTGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGCGTACCAGTTCGCCCAGCGTTTTATCTTTCAGGCACACCAGTTCGGCg                                                                                                        <  2:1516658/150‑1 (MQ=255)
                                              gcCTCACGGCTAAAATGCTGTTCCACGCGGTTAATCGCCTCAACGGGTTGCGCCAGTTTGCTGTTGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGCGTACCAGTTCGCCCAGCGTTTTATCTTTCAGGCACACCAGTTCGGCg                                                                                                        <  1:3205942/150‑1 (MQ=255)
                                               ccTCACGGCTAAAATGCTGTTCCACGCGGTTAATCGCCTCAACGGGTTGCGCCAGTTTGCTGTTGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGCGTACCAGTTCGCCCAGCGTTTTATCTTTCAGGCACACCAGTTCGGCGa                                                                                                       >  2:1978136/1‑150 (MQ=255)
                                               ccTCACGGCTAAAATGCTGTTCCACGCGGTTAATCGCCTCAACGGGTTGCGCCAGTTTGCTGTTGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGCGTACCAGTTCGCCCAGCGTTTTATCTTTCAGGCACACCAGTTCGGCGa                                                                                                       >  2:4701254/1‑150 (MQ=255)
                                                cTCACGGCTAAAATGCTGTTCCACGCGGTTAATCGCCTCAACGGGTTGCGCCAGTTTGCTGTTGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGCGTACCAGTTCGCCCAGCGTTTTATCTTTCAGGCACACCAGTTCGGCGAc                                                                                                      >  1:290382/1‑150 (MQ=255)
                                                cTCACGGCTAAAATGCTGTTCCACGCGGTTAATCGCCTCAACGGGTTGCGCCAGTTTGCTGTTGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGCGTACCAGTTCGCCCAGCGTTTTATCTTTCAGGCACACCAGTTCGGCGAc                                                                                                      >  1:4617392/1‑150 (MQ=255)
                                                cTCACGGCTAAAATGCTGTTCCACGCGGTTAATCGCCTCAACGGGTTGCGCCAGTTTGCTGTTGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGCGTACCAGTTCGCCCAGCGTTTTATCTTTCAGGCACACCAGTTCGGCGAc                                                                                                      <  2:3735722/150‑1 (MQ=255)
                                                cTCACGGCTAAAATGCTGTTCCACGCGGTTAATCGCCTCAACGGGTTGCGCCAGTTTGCTGTTGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGCGTACCAGTTCGCCCAGCGTTTTATCTTTCAGGCACACCAGTTCGGCGAc                                                                                                      >  1:900043/1‑150 (MQ=255)
                                                cTCACGGCTAAAATGCTGTTCCACGCGGTTAATCGCCTCAACGGGTTGCGCCAGTTTGCTGTTGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGAGTACCAGTTCGCCCAGCGTTTTATCTTTCAGGCACACCAGTTCGGCGAc                                                                                                      >  1:331167/1‑150 (MQ=255)
                                                 tCACGGCTAAAATGCTGTTCCACGCGGTTAATCGCCTCAACGGGTTGCGCCAGTTTGCTGTTGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGAGTACCAGTTCGCCCAGCGTTTTATCTTTCAGGCACACCAGTTCGGCGAcc                                                                                                     >  2:678340/1‑150 (MQ=255)
                                                   aCGGCTAAAATGCTGTTCCACGCGGTTAATCGCCTCAACGGGTTGCGCCAGTTTGCTGTTGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGAGTACCAGTTCGCCCAGCGTTTTATCTTTCAGGCACACCAGTTCGGCGACcag                                                                                                   <  2:3695595/150‑1 (MQ=255)
                                                   aCGGCTAAAATGCTGTTCCACGCGGTTAATCGCCTCAACGGGTTGCGCCAGTTTGCTGTTGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGAGTACCAGTTCGCCCAGCGTTTTATCTTTCAGGCACACCAGTTCGGCGACcag                                                                                                   <  2:5122649/150‑1 (MQ=255)
                                                     ggCTAAAATGCTGTTCCACGCGGTTAATCGCCTCAACGGGTTGCGCCAGTTTGCTGTTGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGCGTACCAGTTCGCCCAGCGTTTTATCTTTCAGGCACACCAGTTCGGCGACcagca                                                                                                 <  2:2751520/150‑1 (MQ=255)
                                                     ggCTAAAATGCTGTTCCACGCGGTTAATCGCCTCAACGGGTTGCGCCAGTTTGCTGTTGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGAGTACCAGTTCGCCCAGCGTTTTATCTTTCAGGCACACCAGTTCGGCGACcagca                                                                                                 >  1:1483874/1‑150 (MQ=255)
                                                        tAAAATGCTGTTCCACGCGGTTAATCGCCTCAACGGGTTGCGCCAGTTTGCTGTTGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGCGTACCAGTTCGCCCAGCGTTTTATCTTTCAGGCACACCAGTTCGGCGACCAGCAGcc                                                                                              <  1:996885/150‑1 (MQ=255)
                                                         aaaaTGCTGTTCCACGCGGTTAATCGCCTCAACGGGTTGCGCCAGTTTGCTGTTGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGCGTACCAGTTCGCCCAGCGTTTTATCTTTCAGGCACACCAGTTCGGCGACCAGCAGCCa                                                                                             <  1:4939778/150‑1 (MQ=255)
                                                         aaaaTGCTGTTCCACGCGGTTAATCGCCTCAACGGGTTGCGCCAGTTTGCTGTTGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGAGTACCAGTTCGCCCAGCGTTTTATCTTTCAGGCACACCAGTTCGGCGACCAGCAGCCa                                                                                             >  1:2575836/1‑150 (MQ=255)
                                                           aaTGCTGTTCCACGCGGTTAATCGCCTCAACGGGTTGCGCCAGTTTGCTGTTGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGCGTACCAGTTCGCCCAGCGTTTTATCTTTCAGGCACACCAGTTCGGCGACCAGCAGCCACg                                                                                           >  1:3765839/1‑150 (MQ=255)
                                                           aaTGCTGTTCCACGCGGTTAATCGCCTCAACGGGTTGCGCCAGTTTGCTGTTGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGCGTACCAGTTCGCCCAGCGTTTTATCTTTCAGGCACACCAGTTCGGCGACCAGCAGCCACg                                                                                           >  2:1341924/1‑150 (MQ=255)
                                                              gCTGTTCCACGCGGTTAATCGCCTCAACGGGTTGCGCCAGTTTGCTGTTGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGAGTACCAGTTCGCCCAGCGTTTTATCTTTCAGGCACACCAGTTCGGCGACCAGCAGCCACGGGa                                                                                        >  1:1600970/1‑150 (MQ=255)
                                                                tGTTCCACGCGGTTAATCGCCTCAACGGGTTGCGCCAGTTTGCTGTTGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGCGTACCAGTTCGCCCAGCGTTTTATCTTTCAGGCACACCAGTTCGGCGACCAGCAGCCACGGGATc                                                                                      >  2:2515742/1‑150 (MQ=255)
                                                                tGTTCCACGCGGTTAATCGCCTCAACGGGTTGCGCCAGTTTGCTGTTGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGAGTACCAGTTCGCCCAGCGTTTTATCTTTCAGGCACACCAGTTCGGCGACCAGCAGCCACGGGATc                                                                                      >  1:835857/1‑150 (MQ=255)
                                                                  ttCCTCGCGGTTAATCGCCTCAACGGGTTGCGCCAGTTTGCTGTTGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGAGTACCAGTTCGCCCAGCGTTTTATCTTTCAGGCACACCAGTTCGGCGACCAGCAGCCACGGGATCAt                                                                                    <  2:1215418/150‑1 (MQ=255)
                                                                   tCCACGCGGTTAATCGCCTCAACGGGTTGCGCCAGTTTGCTGTTGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGAGTACCAGTTCGCCCAGCGTTTTATCTTTCAGGCACACCAGTTCGGCGACCAGCAGCCACGGGATCATg                                                                                   >  2:3412506/1‑150 (MQ=255)
                                                                     taCGCGGTTAATCGCCTCAACGGGTTGCGCCAGTTTGCTGTTGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGCGTACCAGTTCGCCCAGCGTTTTATCTTTCAGGCACACCAGTTCGGCGACCAGCAGCCACGGGATCATGcc                                                                                 >  2:419317/2‑150 (MQ=255)
                                                                         cgGTTAATCGCCTCAACGGGTTGCGCCAGTTTGCTGTTGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGCGTACCAGTTCGCCCAGCGTTTTATCTTTCAGGCACACCAGTTCGGCGACCAGCAGCCACGGGATCATGCCGCTg                                                                             >  2:1808512/1‑150 (MQ=255)
                                                                          ggTTAATCGCCTCAACGGGTTGCGCCAGTTTGCTGTTGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGAGTACCAGTTCGCCCAGCGTTTTATCTTTCAGGCACACCAGTTCGGCGACCAGCAGCCACGGGATCATGCCGCTGt                                                                            >  2:4435059/1‑150 (MQ=255)
                                                                           gTTAATCGCCTCAACGGGTTGCGCCAGTTTGCTGTTGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGCGTACCAGTTCGCCCAGCGTTTTATCTTTCAGGCACACCAGTTCGGCGACCAGCAGCCACGGGATCATGCCGCTGTc                                                                           <  1:4588950/150‑1 (MQ=255)
                                                                             tAATCGCCTCAACGGGTTGCGCCAGTTTGCTGTTGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGAGTACCAGTTCGCCCAGCGTTTTATCTTTCAGGCACACCAGTTCGGCGACCAGCAGCCACGGGATCATGCCGCTGTCGc                                                                         <  2:3287208/150‑1 (MQ=255)
                                                                               aTCGCCTCAACGGGTTGCGCCAGTTTGCTGTTGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGCGTACCAGTTCGCCCAGCGTTTTATCTTTCAGGCACACCAGTTCGGCGACCAGCAGCCACGGGATCATGCCGCTGTCGCAg                                                                       >  1:2685170/1‑150 (MQ=255)
                                                                                   ccTCAACGGGTTGCGCCAGTTTGCTGTTGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGCGTACCAGTTCGCCCAGCGTTTTATCTTTCAGGCACACCAGTTCGGCGACCAGCAGCCACGGGATCATGCCGCTGTCGCAGTAAg                                                                   <  1:725591/150‑1 (MQ=255)
                                                                                      cAACGGGTTGCGCCAGTTTGCTGTTGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGCGTACCAGTTCGCCCAGCGTTTTATCTTTCAGGCACACCAGTTCGGCGACCAGCAGCCACGGGATCATGCCGCTGTCGCAGTAAGCGa                                                                >  2:3118638/1‑150 (MQ=255)
                                                                                        aCGGGTTGCGCCAGTTTGCTGTTGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGCGTACCAGTTCGCCCAGCGTTTTATCTTTCAGGCACACCAGTTCGGCGACCAGCAGCCACGGGATCATGCCGCTGTCGCAGTAAGCGaaa                                                              >  2:2644676/1‑150 (MQ=255)
                                                                                         cGGGTTGCGCCAGTTTGCTGTTGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGCGTACCAGTTCGCCCAGCGTTTTATCTTTCAGGCACACCAGTTCGGCGACCAGCAGCCACGGGATCATGCCGCTGTCGCAGTAAGCGAAAt                                                             <  2:663720/150‑1 (MQ=255)
                                                                                           ggTTGCGCCAGTTTGCTGTTGATCTCACCGATTGCCGGAAACGCCGCCATCCGGTCGAGTACCAGTTCGCCCAGCGTTTTATCTTTCAGTCACACCAGTTCGGCGACCAGCAGCCACGGGATCATGCCGCTGTCGCAGTAATCGAAATCa                                                           <  1:2427413/150‑1 (MQ=255)
                                                                                             ttGCGCCAGTTTGCTGTTGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGCGTACCAGTTCGCCCAGCGTTTTATCTTTCAGGCACACCAGTTCGGCGACCAGCAGCCACGGGATCATGCCGCTGTCGCAGTAAGCGAAATCACg                                                         <  1:3922562/150‑1 (MQ=255)
                                                                                              tGCGCCAGTTTGCTGTTGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGAGTACCAGTTCGCCCAGCGTTTTATCTTTCAGGCACACCAGTTCGGCGACCAGCAGCCACGGGATCATGCCGCTGTCGCAGTAAGCGAAATCACgg                                                        >  1:1611324/1‑150 (MQ=255)
                                                                                                    aGTTTGCTGTTGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGCGTACCAGTTCGCCCAGCGTTTTATCTTTCAGGCACACCAGTTCGGCGACCAGCAGCCACGGGATCATGCCGCTGTCGCAGTAAGCGAAATCACGGAAGTaa                                                  <  2:1895128/150‑1 (MQ=255)
                                                                                                        tGCTGTTGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGAGTACCAGTTCGCCCAGCGTTTTATCTTTCAGGCACACCAGTTCGGCGACCAGCAGCCACGGGATCATGCCGCTGTCGCAGTAAGCGAAATCACGGAAGTAAtggt                                              <  2:5143120/150‑1 (MQ=255)
                                                                                                         gCTGTTGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGAGTACCAGTTCGCCCAGCGTTTTATCTTTCAGGCACACCAGTTCGGCGACCAGCAGCCACGGGATCATGCCGCTGTCGCAGTAAGCGAAATCACGGAAGTAAtggtg                                             <  2:228484/150‑1 (MQ=255)
                                                                                                            gTTGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGCGTACCAGTTCGCCCAGCGTTTTATCTTTCAGGCACACCAGTTCGGCGACCAGCAGCCACGGGATCATGCCGCTGTCGCAGTAAGCGAAATCACGGAAGTAATGGTGGgc                                          <  1:3082039/150‑1 (MQ=255)
                                                                                                             ttGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGCGTACCAGTTCGCCCAGCGTTTTATCTTTCAGGCACACCAGTTCGGCGACCAGCAGCCACGGGATCATGCCGCTGTCGCAGTAAGCGAAATCACGGAAGTAATGGTGGgcg                                         <  2:88459/150‑1 (MQ=255)
                                                                                                             ttGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGCGTACCAGTTCGCCCAGCGTTTTATCTTTCAGGCACACCAGTTCGGCGACCAGCAGCCACGGGATCATGCCGCTGTCGCAGTAAGCGAAATCACGGAAGTAATGGTGGgcg                                         <  2:3672895/150‑1 (MQ=255)
                                                                                                               gATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGAGTACCAGTTCGCCCAGCGTTTTATCTTTCAGGCACACCAGTTCGGCGACCAGCAGCCACGGGATCATGCCGCTGTCGCAGTAAGCGAAATCACGGAAGTAATGGTGGGCGCt                                       >  1:2543403/1‑150 (MQ=255)
                                                                                                                aTCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGAGTACCAGTTCGCCCAGCGTTTTATCTTTCAGGCACACCAGTTCGGCGACCAGCAGCCACGGGATCATGCCGCTGTCGCAGTAAGCGAAATCACGGAAGTAATGGTGGGCGCTc                                      <  1:1340158/150‑1 (MQ=255)
                                                                                                                 tctcACCGCTTGCCGGAAACGCCGCCATCCGGTCGCGTACCAGTTCGCCCAGCGTTTTATCTTTCAGGCACACCAGTTCGGCGACCAGCAGCCACGGGATCATGCCGCTGTCGCAGTAAGCGAAATCACGGAAGTAATGGTGGGCGCTCa                                     >  2:1122621/1‑150 (MQ=255)
                                                                                                                  ctcACCGCTTGCCGGAAACGCCGCCATCCGGTCGCGTACCAGTTCGCCCAGCGTTTTATCTTTCAGGCACACCAGTTCGGCGACCAGCAGCCACGGGATCATGCCGCTGTCGCAGTAAGCGAAATCACGGAAGTAATGGTGGGCGCTCAt                                    <  1:1259170/150‑1 (MQ=255)
                                                                                                                   tcACCGCTTGCCGGAAACGCCGCCATCCGGTCGCGTACCAGTTCGCCCAGCGTTTTATCTTTCAGGCACACCAGTTCGGCGACCAGCAGCCACGGGATCATGCCGCTGTCGCAGTAAGCGAAATCACGGAAGTAATGGTGGGCGCTCAtt                                   >  1:1928045/1‑150 (MQ=255)
                                                                                                                      ccGCTTGCCGGAAACGCCGCCATCCGGTCGAGTACCAGTTCGCCCAGCGTTTTATCTTTCAGGCACACCAGTTCGGCGACCAGCAGCCACGGGATCATGCCGCTGTCGCAGTAAGCGAAATCACGGAAGTAATGGTGGGCGCTCATTTCg                                >  1:1935736/1‑150 (MQ=255)
                                                                                                                        gCTTGCCGGAAACGCCGCCATCCGGTCGAGTACCAGTTCGCCCAGCGTTTTATCTTTCAGGCACACCAGTTCGGCGACCAGCAGCCACGGGATCATGCCGCTGTCGCAGTAAGCGAAATCACGGAAGTAATGGTGGGCGCTCATTTCGcc                              <  2:1483832/150‑1 (MQ=255)
                                                                                                                               ggAAACGCCGCCATCCGGTCGCGTACCAGTTCGCCCAGCGTTTTATCTTTCAGGCACACCAGTTCGGCGACCAGCAGCCACGGGATCATGCCGCTGTCGCAGTAAGCGAAATCACGGAAGTAATGGTGGGCGCTCATTTCGCCACCATAg                       >  1:2231699/1‑150 (MQ=255)
                                                                                                                               ggAAACGCCGCCATCCGGTCGCGTACCAGTTCGCCCAGCGTTTTATCTTTCAGGCACACCAGTTCGGCGACCAGCAGCCACGGGATCATGCCGCTGTCGCAGTAAGCGAAATCACGGAAGTAATGGTGGGCGCTCATTTCGCCACCATAg                       <  2:4009067/150‑1 (MQ=255)
                                                                                                                                gAAACGCCGCCATCCGGTCGCGTACCAGTTCGCCCAGCGTTTTATCTTTCAGGCACACCAGTTCGGCGACCAGCAGCCACGGGATCATGCCGCTGTCGCAGTAAGCGAAATCACGGAAGTAATGGTGGGCGCTCATTTCGCCACCATAGa                      >  1:2339297/1‑150 (MQ=255)
                                                                                                                                 aaaCGCCGCCATCCGGTCGCGTACCAGTTCGCCCAGCGTTTTATCTTTCAGGCACACCAGTTCGGCGACCAGCAGCCACGGGATCATGCCGCTGTCGCAGTAAGCGAAATCACGGAAGTAATGGTGGGCGCTCATTTCGCCACCATAGAt                     >  2:1032356/1‑150 (MQ=255)
                                                                                                                                 aaaCGCCGCCATCCGGTCGAGTACCAGTTCGCCCAGCGTTTTATCTTTCAGGCACACCAGTTCGGCGACCAGCAGCCACGGGATCATGCCGCTGTCGCAGTAAGCGAAATCACGGAAGTAATGGTGGGCGCTCATTTCGCCACCATAGAt                     >  1:2303540/1‑150 (MQ=255)
                                                                                                                                  aaCGCCGCCATCCGGTCGCGTACCAGTTCGCCCAGCGTTTTATCTTTCAGGCACACCAGTTCGGCGACCAGCAGCCACGGGATCATGCCGCTGTCGCAGTAAGCGAAATCACGGAAGTAATGGTGGGCGCTCATTTCGCCACCATAGATg                    <  2:4889179/150‑1 (MQ=255)
                                                                                                                                        gccATCCGGTCGCGTACCAGTTCGCCCAGCGTTTTATCTTTCAGGCACACCAGTTCGGCGACCAGCAGCCACGGGATCATGCCGCTGTCGCAGTAAGCGAAATCACGGAAGTAATGGTGGGCGCTCATTTCGCCACCATAGATGGCGTCt              <  1:4255797/150‑1 (MQ=255)
                                                                                                                                        gccATCCGGTCGCGTACCAGTTCGCCCAGCGTTTTATCTTTCAGGCACACCAGTTCGGCGACCAGCAGCCACGGGATCATGCCGCTGTCGCAGTAAGCGAAATCACGGAAGTAATGGTGGGCGCTCATTTCGCCACCATAGATGGCGTCt              <  1:5100243/150‑1 (MQ=255)
                                                                                                                                         ccATCCGGTCGCGTACCAGTTCGCCCAGCGTTTTATCTTTCAGGCACACCAGTTCGGCGACCAGCAGCCACGGGATCATGCCGCTGTCGCAGTAAGCGAAATCACGGAAGTAATGGTGGGCGCTCATTTCGCCACCATAGATGGCGTCtt             <  1:2644676/150‑1 (MQ=255)
                                                                                                                                            tCCGGTCGCGTACCAGTTCGCCCAGCGTTTTATCTTTCAGGCACACCAGTTCGGCGACCAGCAGCCACGGGATCATGCCGCTGTCGCAGTAAGCGAAATCACGGAAGTAATGGTGGGCGCTCATTTCGCCACCATAGATGGCGTCTTCCt          >  1:2068225/1‑150 (MQ=255)
                                                                                                                                            tCCGGTCGCGTACCAGTTCGCCCAGCGTTTTATCTTTCAGGCACACCAGTTCGGCGACCAGCAGCCACGGGATCATGCCGCTGTCGCAGTAAGCGAAATCACGGAAGTAATGGTGGGCGCTCATTTCGCCACCATAGATGGCGTCTTCCt          <  1:1877564/150‑1 (MQ=255)
                                                                                                                                            tCCGGTCGAGTACCAGTTCGCCCAGCGTTTTATCTTTCAGGCACACCAGTTCGGCGACCAGCAGCCACGGGATCATGCCGCTGTCGCAGTAAGCGAAATCACGGAAGTAATGGTGGGCGCTCATTTCGCCACCATAGATGGCGTCTTCCt          <  2:1670836/150‑1 (MQ=255)
                                                                                                                                              cGGTCGCGTACCAGTTCGCCCAGCGTTTTATCTTTCAGGCACACCAGTTCGGCGACCAGCAGCCACGGGATCATGCCGCTGTCGCAGTAAGCGAAATCACGGAAGTAATGGTGGGCGCTCATTTCGCCACCATAGATGGCGTCTTCCTTg        <  2:3830007/150‑1 (MQ=255)
                                                                                                                                               ggTCGCGTACCAGTTCGCCCAGCGTTTTATCTTTCAGGCACACCAGTTCGGCGACCAGCAGCCACGGGATCATGCCGCTGTCGCAGTAAGCGAAATCACGGAAGTAATGGTGGGCGCTCATTTCGCCACCATAGATGGCGTCTTCCTTgc       <  2:2599578/150‑1 (MQ=255)
                                                                                                                                               ggTCGAGTACCAGTTCGCCCAGCGTTTTATCTTTCAGGCACACCAGTTCGGCGACCAGCAGCCACGGGATCATGCCGCTGTCGCAGTAAGCGAAATCACGGAAGTAATGGTGGGCGCTCATTTCGCCACCATAGATGGCGTCTTCCTTgc       >  1:2487331/1‑150 (MQ=255)
                                                                                                                                                    cgTACCAGTTCGCCCAGCGTTTTATCTTTCAGGCACACCAGTTCGGCGACCAGCAGCCACGGGATCATGCCGCTGTCGCAGTAAGCGAAATCACGGAAGTAATGGTGGGCGCTCATTTCGCCACCATAGATGGCGTCTTCCTTGCGCATa  <  2:1465825/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
GTCGGCAAAGGTCATGCTGATGCCATCGGTGCGATCCACCGCCAGCGCCTCACGGCTAAAATGCTGTTCCACGCGGTTAATCGCCTCAACGGGTTGCGCCAGTTTGCTGTTGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGCGTACCAGTTCGCCCAGCGTTTTATCTTTCAGGCACACCAGTTCGGCGACCAGCAGCCACGGGATCATGCCGCTGTCGCAGTAAGCGAAATCACGGAAGTAATGGTGGGCGCTCATTTCGCCACCATAGATGGCGTCTTCCTTGCGCATA  >  CP009273/2115213‑2115510

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑