BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	2,146,030	T→A	10.1%	S194C (AGT→TGT)	yegL ←	VMA domain protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	2,146,030	0	T	A	10.1%	1135.9 / 89.9	344	S194C (AGT→TGT)	yegL	VMA domain protein
Reads supporting (aligned to +/- strand): ref base T (119/190); new base A (18/17); total (137/207)
Fisher's exact test for biased strand distribution p-value = 1.49e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 349 total aligned reads displayed.

CATCCTGACAAGGCAAGTCCGCGCTGATGTGCGACGTTCCAACGGTTGATGCATAGACCAGACGCCAGCTCACACTGACGTCCAGCCTTTTGGCGCTTCCAGCACAACTTCCGTTCCCGGCGTGGAGCGGGAAACCGAACGCAGCGAACTGGATAACCAGCTAAACAGTTCACGGAATTGTAATCCTTGCAGAGGCAAAGGCTGACGAACGCTAATTTGTGCCAGCGTCTTCATATCCGCCCCCTGAACACCGATGGAAAAGAAAGCAAACCTTTTATCTTCTTCCCCCCGAAACACTT  >  CP009273/2145881‑2146179

caTCCTGACAAGGCAAGTCCGCGCTGATGTGCGACGTTCCAACGGTTGATGCATAGACCAGACGCCAGCTCACACTGACGTCCAGCCTTTTGGCGCTTCCAGCACAACTTCCGTTCCCGGCGTGGAGCGGGAAACCGAACGCAGCGAACt                                                                                                                                                       <  1:4256869/150‑1 (MQ=255)

    cTGACAAGGCAAGTCCGCGCTGATGTGCGACGTTCCAACGGTTGATGCATAGACCAGACGCCAGCTCACACTGACGTCCAGCCTTTTGGCGCTTCCAGCACAACTTCCGTTCCCGGCGTGGAGCGGGAAACCGAACGCAGCGAACTGGAt                                                                                                                                                   >  2:1617156/1‑150 (MQ=255)

      gACAAGGCAAGTCCGCGCTGATGTGCGACGTTCCAACGGTTGATGCATAGACCAGACGCCAGCTCACACTGACGTCCAGCCTTTTGGCGCTTCCAGCACAACTTCCGTTCCCGGCGTGGAGCGGGACACCGAACGCAGCGAACTGGATaa                                                                                                                                                 >  2:1813589/1‑150 (MQ=255)

      gACAAGGCAAGTCCGCGCTGATGTGCGACGTTCCAACGGTTGATGCATAGACCAGACGCCAGCTCACACTGACGTCCAGCCTTTTGGCGCTTCCAGCACAACTTCCGTTCCCGGCGTGGAGCGGGAAACCGAACGCAGCGAACTGGATaa                                                                                                                                                 <  1:4750411/150‑1 (MQ=255)

      gACAAGGCAAGTCCGCGCTGATGTGCGACGTTCCAACGGTTGATGCATAGACCAGACGCCAGCTCACACTGACGTCCAGCCTTTTGGCGCTTCCAGCACAACTTCCGTTCCCGGCGTGGAGCGGGAAACCGAACGCAGCGAACTGGATaa                                                                                                                                                 <  2:2627507/150‑1 (MQ=255)

      gACAAGGCAAGTCCGCGCTGATGTGCGACGTTCCAACGGTTGATGCATAGACCAGACGCCAGCTCACACTGACGTCCAGCCTTTTGGCGCTTCCAGCACAACTTCCGTTCCCGGCGTGGAGCGGGAAACCGAACGCAGCGAACTGGATaa                                                                                                                                                 >  2:3332109/1‑150 (MQ=255)

       aCAAGGCAAGTCCGCGCTGATGTGCGACGTTCCAACGGTTGATGCATAGACCAGACGCCAGCTCACACTGACGTCCAGCCTTTTGGCGCTTCCAGCACAACTTCCGTTCCCGGCGTGGAGCGGGAAACCGAACGCAGCGAACTGGATAAc                                                                                                                                                <  2:2274450/150‑1 (MQ=255)

       aCAAGGCAAGTCCGCGCTGATGTGCGACGTTCCAACGGTTGATGCATAGACCAGACGCCAGCTCACACTGACGTCCAGCCTTTTGGCGCTTCCAGCACAACTTCCGTTCCCGGCGTGGAGCGGGAAACCGAACGCAGCGAACTGGATAAc                                                                                                                                                <  2:3271701/150‑1 (MQ=255)

        cAAGGCTAGTCCGCGCTGATGTGCGACGTTCCAACGGTTGATGCATAGACCAGACGCCAGCTCACACTGACGTCCAGCCTTTTGGCGCTTCCAGCACAACTTCCGTTCCCGGCGTGGAGCGGGAAACCGAACGCAGCGAACTGGATAAcc                                                                                                                                               <  2:1432240/150‑1 (MQ=255)

        cAAGGCAAGTCCGCGCTGATGTGCGACGTTCCAACGGTTGATGCATAGACCAGACGCCAGCTCACACTGACGTCCAGCCTTTTGGCGCTTCCAGCACAACTTCCGTTCCCGGCGTGGAGCGGGAAACCGAACGCAGCGAACTGGATAAcc                                                                                                                                               <  1:2742128/150‑1 (MQ=255)

           ggCAAGTCCGCGCTGATGTGCGACGTTCCAACGGTTGATGCATAGACCAGACGCCAGCTCACACTGACGTCCAGCCTTTTGGCGCTTCCAGCACAACTTCCGTTCCCGGCGTGGAGCGGGAAACCGAACGCAGCGAACTGTATAACCAGc                                                                                                                                            <  2:4910646/150‑1 (MQ=255)

            gCAAGTCCGCGCTGATGTGCGACGTTCCAACGGTTGATGCATAGACCAGACGCCAGCTCACACTGACGTCCAGCCTTTTGGCGCTTCCAGCACAACTTCCGTTCCCGGCGTGGAGCGGGAAACCGAACGCAGCGAACTGGATAACCAGCt                                                                                                                                           <  1:3510003/150‑1 (MQ=255)

             cAAGTCCGCGCTGATGTGCGACGTTCCAACGGTTGATGCATAGACCAGACGCCATCTCACACTGACGTCCAGCCTTTTGGCGCTTACAGCACAACTTCCGTTCCCGGCGTGGAGCGGGAAACCGAACGCAGCGAACTGGATAACCAGCTa                                                                                                                                          <  1:653481/150‑1 (MQ=255)

              aaGTCCGCGCTGATGTGCGACGTTCCAACGGTTGATGCATAGACCAGACGCCAGCTCACACTGACGTCCAGCCTTTTGGCGCTTCCAGCACAACTTCCGTTCCCGGCGTGGAGCGGGAAACCGAACGCAGCGAACTGGATAACCAGCTaa                                                                                                                                         >  2:3526829/1‑150 (MQ=255)

                gTCCGCGCTGATGTGCGACGTTCCAACGGTTGATGCATAGACCAGACGCCAGCTCACACTGACGTCCAGCCTTTTGGCGCTTCCAGCACAACTTCCGTTCCCGGCGTGGAGCGGGAAACCGAACGCAGCGAACTGGATAACCAGCTAAAc                                                                                                                                       <  1:1133973/150‑1 (MQ=255)

                    gcgcTGATGTGCGACGTTCCAACGGTTGATGCATAGACCAGACGCCAGCTCACACTGACGTCCAGCCTTTTGGCGCTTCCAGCACAACTTCCGTTCCCGGCGTGGAGCGGGAAACCGAACGCAGCGAACTGGATAACCAGCTAAACAGtt                                                                                                                                   >  2:1772398/1‑150 (MQ=255)

                       cTGATGTGCGACGTTCCAACGGTTGATGCATAGACCAGACGCCAGCTCACACTGACGTCCAGCCTTTTGGCGCTTCCAGCACAACTTCCGTTCCCGGCGTGGAGCGGGAAACCGAACGCAGCGAACAGGATAACCAGCTAAACAGTTCAc                                                                                                                                >  2:4830664/1‑150 (MQ=255)

                            gtgCGACGTTCCAACGGTTGATGCATAGACCAGACGCCAGCTCACACTGACGTCCAGCCTTTTGGCGCTTCCAGCACAACTTCCGTTCCCGGCGTGGAGCGGGAAACCGAACGCAGCGAACTGGATAACCAGCTAAACAGTTCACGGAAt                                                                                                                           >  2:676259/1‑150 (MQ=255)

                                gACGTTCCAACGGTTGATGCATAGACCAGACGCCAGCTCACACTGACGTCCAGCCTTTTGGCGCTTCCAGCACAACTTCCGTTCCCGGCGTGGAGCGGGAAACCGAACGCAGCGAACTGGATAACCAGCTAAACAGTTCACGGAATTGTa                                                                                                                       <  2:4993564/150‑1 (MQ=255)

                                gACGTTCCAACGGTTGATGCATAGACCAGACGCCAGCTCACACTGACGTCCAGCCTTTTGGCGCTTCCAGCACAACTTCCGTTCCCGGCGTGGAGCGGGAAACCGAACGCAGCGAACTGGATAACCAGCTAAACAGTTCACGGAATTGTa                                                                                                                       <  1:3933555/150‑1 (MQ=255)

                                     tCCAACGGTTGATGCATAGACCAGACGCCAGCTCACACTGACGTCCAGCCTTTTGGCGCTTCCAGCACAACTTCCGTTCCCGGCGTGGAGCGGGAAACCGAACGCAGCGAACTGGATAACCAGCTAAACAGTTCACGGAATTGTAATCCt                                                                                                                  <  2:272899/150‑1 (MQ=255)

                                     tCCAACGGTTGATGCATAGACCAGACGCCAGCTCACACTGACGTCCAGCCTTTTGGCGCTTCCAGCACAACTTCCGTTCCCGGCGTGGAGCGGGAAACCGAACGCAGCGAACTGGATAACCAGCTAAACAGTTCACGGAATTGTAATCCt                                                                                                                  <  2:387340/150‑1 (MQ=255)

                                      ccAACGGTTGATGCATAGACCAGACGCCAGCTCACACTGACGTCCAGCCTTTTGGCGCTTCCAGCACAACTTCCGTTCCCGGCGTGGAGCGGGAAACCGAACGCAGCGAACTGGATAACCAGCTAAACAGTTCACGGAATTGTAATCCtt                                                                                                                 <  1:909969/150‑1 (MQ=255)

                                       cAACGGTTGATGCATAGACCAGACGCCAGCTCACACTGACGTCCAGCCTTTTGGCGCTTCCAGCACAACTTCCGTTCCCGGCGTGGAGCGGGAAACCGAACGCAGCGAACTGGATAACCAGCTAAACAGTTCACGGAATTGTAATCCTTg                                                                                                                <  2:3389785/150‑1 (MQ=255)

                                        aaCGGTTGATGCATAGACCAGACGCCAGCTCACACTGACGTCCAGCCTTTTGGCGCTTCCAGCACAACTTCCGTTCCCGGCGTGGAGCGGGAAACCGAACGCAGCGAACTGGATAACCAGCTAAACAGTTCACGGAATTGTAATCCTTGc                                                                                                               >  2:4522079/1‑150 (MQ=255)

                                         aCGGTTGATGCATAGACCAGACGCCAGCTCACACTGACGTCCAGCCTTTTGGCGCTTCCAGCACAACTTCCGTTCCCGGCGTGGAGCGGGAAACCGAACGCAGCGAACTGGATAACCAGCTAAACAGTTCACGGAATTGTAATCCTTGCa                                                                                                              >  2:5095001/1‑150 (MQ=255)

                                             ttGATGCATAGACCAGACGCCAGCTCACACTGACGTCCAGCCTTTTGGCGCTTCCAGCACAACTTCCGTTCCCGGCGTGGAGCGGGAAACCGAACGCAGCGAACTGGATAACCAGCTAAACAGTTCACGGAATTGTAATCCTTGCAGAgg                                                                                                          >  2:830423/1‑150 (MQ=255)

                                             ttGATGCATAGACCAGACGCCAGCTCACACTGACGTCCAGCCTTTTGGCGCTTCCAGCACAACTTCCGTTCCCGGCGTGGAGCGGGAAACCGAACGCAGCGAACTGGATAACCAGCTAAACAGTTCACGGAATTGTAATCCTTGCAGAgg                                                                                                          <  2:1208109/150‑1 (MQ=255)

                                             ttGATGCATAGACCAGACGCCAGCTCACACTGACGTCCAGCCTTTTGGCGCTTCCAGCACAACTTCCGTTCCCGGCGTGGAGCGGGAAACCGAACGCAGCGAACTGGATAACCAGCTAAACAGTTCACGGAATTGTAATCCTTGCAGAgg                                                                                                          >  2:3407659/1‑150 (MQ=255)

                                              tGATGCATAGACCAGACGCCAGCTCACACTGACGTCCAGCCTTTTGGCGCTTCCAGCACAACTTCCGTTCCCGGCGTGGAGCGGGAAACCGAACGCAGCGAACTGGATAACCAGCTAAACAGTTCACGGACTTGTAATCCTTGCAGAGGc                                                                                                         >  2:4780304/1‑150 (MQ=255)

                                              tGATGCATAGACCAGACGCCAGCTCACACTGACGTCCAGCCTTTTGGCGCTTCCAGCACAACTTCCGTTCCCGGCGTGGAGCGGGAAACCGAACGCAGCGAACTGGATAACCAGCTAAACAGTTCACGGAATTGTAATCCTTGCAGAGGc                                                                                                         >  2:3666810/1‑150 (MQ=255)

                                              tGATGCATAGACCAGACGCCAGCTCACACTGACGTCCAGCCTTTTGGCGCTTCCAGCACAACTTCCGTTCCCGACGTGGAGCGGGAAACCGAACGCAGCGAACTGGATAACCAGCTAAACAGTTCACGGAATTGTAATCCTTGCAGAGGc                                                                                                         <  2:791457/150‑1 (MQ=255)

                                               gATGCATAGACCAGACGCCAGCTCACACTGACGTCCAGCCTTTTGGCGCTTCCAGCACAACTTCCGTTCCCGGCGTGGAGCGGGAAACCGAACGCAGCGAACTGGATAACCAGCTAAACAGTTCACGGAATTGTAATCCTTGCAGAGGCa                                                                                                        <  1:3115732/150‑1 (MQ=255)

                                                  gCATAGACCAGACGCCAGCTCACACTGACGTCCAGCCTTTTGGCGCTTCCAGCACAACTTCCGTTCCCGGCGTGGAGCGGGAAACCGAACGCAGCGAACTGGATAACCAGCTAAACAGTTCACGGAATTGTAATCCTTGCAGAGGCAAAg                                                                                                     <  2:4850237/150‑1 (MQ=255)

                                                     tAGACCAGACGCCAGCTCACACTGACGTCCAGCCTTTTGGCGCTTCCAGCACAACTTCCGTTCCCGGCGTGGAGCGGGAAACCGAACGCAGCGAACTGGATAACCAGCTAAACAGTTCACGGAATTGTAATCCTTGCAGAGGCAAAGGCt                                                                                                  <  1:996228/150‑1 (MQ=255)

                                                     tAGACCAGACGCCAGCTCACACTGACGTCCAGCCTTTTGGCGCTTCCAGCACAACTTCCGTTCCCGGCGTGGAGCGGGAAACCGAACGCAGCGAACTGGATAACCAGCTAAACAGTTCACGGAATTGTAATCCTTGCAGAGGCAAAGGCt                                                                                                  <  1:2268430/150‑1 (MQ=255)

                                                      aGACCAGACGCCAGCTCACACTGACGTCCAGCCTTTTGGCGCTTCCAGCACAACTTCCGTTCCCGGCGTGGAGCGGGAAACCGAACGCAGCGAACTGGATAACCAGCTAAACAGTTCACGGAATTGTAATCCTTGCAGAGGCAAAGGCTg                                                                                                 <  1:133163/150‑1 (MQ=255)

                                                      aGACCAGACGCCAGCTCACACTGACGTCCAGCCTTTTGGCGCTTCCAGCACAACTTCCGTTCCCGGCGTGGAGCGGGAAACCGAACGCAGCGAACTGGATAACCAGCTAAACAGTTCACGGAATTGTAATCCTTGCAGAGGCAAAGGCTg                                                                                                 >  1:1842806/1‑150 (MQ=255)

                                                           aGACGCCAGCTCACACTGACGTCCAGCCTTTTGGCGCTTCCAGCACAACTTCCGTTCCCGGCGTGGAGCGGGAAACCGAACGCAGCGAACAGGATAACCAGCTAAACAGTTCACGGAATTGTAATCCTTGCAGAGGCAAAGGCTGACGaa                                                                                            >  2:1727744/1‑150 (MQ=255)

                                                             aCGCCAGCTCACACTGACGTCCAGCCTTTTGGCGCTTCCAGCACAACTTCCGTTCCCGGCGTGGAGCGGGAAACCGAACGCAGCGAACTGGATAACCAGCTAAACAGTTCACGGAATTGTAATCCTTGCAGAGGCAAAGGCTGACGAACg                                                                                          <  2:4097427/150‑1 (MQ=255)

                                                               gCCAGCTCACACTGACGTCCAGCCTTTTGGCGCTTCCAGCACAACTTCCGTTCCCGGCGTGGAGCGGGAAACCGAACGCAGCGAACTGGATAACCAGCTAAACAGTTCACGGAATTGTAATCCTTGCAGAGGCAAAGGCTGACGAACGCt                                                                                        >  1:3900750/1‑150 (MQ=255)

                                                                  aGCTCACACTGACGTCCAGCCTTTTGGCGCTTCCAGCACAACTTCCGTTCCCGGCGTGGAGCGGGAAACCGAACGCAGCGAACTGGATAACCAGCTAAACAGTTCACGGAATTGTAATCCTTGCAGAGGCAAAGGCTGACGAACGCTAAt                                                                                     <  1:1595816/150‑1 (MQ=255)

                                                                   gCTCACACTGACGTCCAGCCTTTTGGCGCTTCCAGCACAACTTCCGTTCCCGGCGTGGAGCGGGAAACCGAACGCAGCGAACTGGATAACCAGCTAAACAGTTCACGGAATTGTAATCCTTGCAGAGGCAAAGGCTGACGAACGCTAAtt                                                                                    <  2:4556669/150‑1 (MQ=255)

                                                                     tCACACTGACGTCCAGCCTTTTGGCGCTTCCAGCACAACTTCCGTTCCCGGCGTGGAGCGGGAAACCGAACGCAGCGAACTGGATAACCAGCTAAACAGTTCACGGAATTGTAATCCTTGCAGAGGCAAAGGCTGACGAACGCTAATTtg                                                                                  >  2:1684236/1‑150 (MQ=255)

                                                                     tCACACTGACGTCCAGCCTTTTGGCGCTTCCAGCACAACTTCCGTTCCCGGCGTGGAGCGGGAAACCGAACGCAGCGAACTGGATAACCAGCTAAACAGTTCACGGAATTGTAATCCTTGCAGAGGCAAAGGCTGACGAACGCTAATTtg                                                                                  <  1:3671786/150‑1 (MQ=255)

                                                                      cacaCTGACGTCCAGCCTTTTGGCGCTTCCAGCACAACTTCCGTTCCCGGCGTGGAGCGGGAAACCGAACGCAGCGAACTGGATAACCAGCTAAACAGTTCACGGAATTGTAATCCTTGCAGAGGCAAAGGCTGACGAACGCTAATTtgt                                                                                 <  2:1171524/150‑1 (MQ=255)

                                                                       acacTGACGTCCAGCCTTTTGGCGCTTCCAGCACAACTTCCGTTCCCGGCGTGGAGCGGGAAACCGAACGCAGCGAACTGGATAACCAGCTAAACAGTTCACGGAATTGTAATCCTTGCAGAGGCAAAGGCTGACGAACGCTAATTtgtg                                                                                <  1:3616176/150‑1 (MQ=255)

                                                                        cacTGACGTCCAGCCTTTTGGCGCTTCCAGCACAACTTCCGTTCCCGGCGTGGAGCGGGAAACCGAACGCAGCGAACTGGATAACCAGCTAAACAGTTCACGGAATTGTAATCCTTGCAGAGGCAAAGGCTGACGAACGCTAATTTGTGc                                                                               >  1:536230/1‑150 (MQ=255)

                                                                           tGACGTCCAGCCTTTTGGCGCTTCCAGCACAACTTCCGTTCCCGGCGTGGAGCGGGAAACCGAACGCAGCGAACTGGATAACCAGCTAAACAGTTCACGGAATTGTAATCCTTGCAGAGGCAAAGGCTGACGAACGCTCATTTGTGCCAg                                                                            >  2:1337192/1‑150 (MQ=255)

                                                                           tGACGTCCAGCCTTTTGGCGCTTCCAGCACAACTTCCGTTCCCGGCGTGGAGCGGGAAACCGAACGCAGCGAACTGGATAACCAGCTAAACAGTTCACGGAATTGTAATCCTTGCAGAGGCAAAGGCTGACGAACGCTAATTTGTGCCAg                                                                            >  1:4542386/1‑150 (MQ=255)

                                                                               gTCCAGCCTTTTGGCGCTTCCAGCACAACTTCCGTTCCCGGCGTGGAGCGGGAAACCGAACGCAGCGAACTGGATAACCAGCTAAACAGTTCACGGAATTGTAATCCTTGCAGAGGCAAAGGCTGACGAACGCTAATTTGTGCCAGCGTc                                                                        >  2:380618/1‑150 (MQ=255)

                                                                                tCCAGCCTTTTGGCGCTTCCAGCACAACTTCCGGTCCCGGCGTGGAGCGGGAAACCGAACGCAGCGAACTGGATAACCAGCTAAACAGTTCACGGAATTGTAACCCTTGCAGAGGCAAAGGCTGACGAACGCTAATTTGTGCCAGCGTCt                                                                       <  2:4468295/150‑1 (MQ=255)

                                                                                 ccAGCCTTTTGGCGCTTCCAGCACAACTTCCGTTCCCGGCGTGGAGCGGGAAACCGAACGCAGCGAACTGGATAACCAGCTAAACAGTTCACGGAATTGTAATCCTTGCAGAGGCAAAGGCTGACGAACGCTAATTTGTGCCAGCGTCtt                                                                      >  1:2860088/1‑150 (MQ=255)

                                                                                   aGCCTTTTGGCGCTTCCAGCACAACTTCCGTTCCCGGCGTGGAGCGGGAAACCGAACGCAGCGAACAGGATAACCAGCTAAACAGTTCACGGAATTGTAATCCTTGCAGAGGCAAAGGCTGACGAACGCTAATTTGTGCCAGCGTCTTCa                                                                    >  1:1358959/1‑150 (MQ=255)

                                                                                      cTTTTGGCGCTTCCAGCACAACTTCCGTTCCCGGCGTGGAGCGGGAAACCGAACGCAGCGAACTGGATAACCAGCTAAACAGTTCACGGAATTGTAATCCTTGCAGAGGCAAAGGCTGACGAACGCTAATTTGTGCCAGCGTCTTCatat                                                                 <  2:1403972/150‑1 (MQ=255)

                                                                                         ttGGCGCTTCCAGCACAACTTCCGTTCCCGGCGTGGAGCGGGAAACCGAACGCAGCGAACTGGATAACCAGCTAAACAGTTCACGGAATTGTAATCCTTGCAGAGGCAAAGGCTGACGAACGCTAATTTGTGCCAGCGTCTTCATATCCg                                                              <  2:2834991/150‑1 (MQ=255)

                                                                                               cTTCCAGCACAACTTCCGTTCCCGGCGTGGAGCGGGAAACCGAACGCAGCGAACTGGATAACCAGCTAAACAGTTCACGGAATTGTAATCCTTGCAGAGGCAAAGGCTGACGAACGCTAATTTGTGCCAGCGTCTTCATATCCGCCCCCt                                                        <  1:3149381/150‑1 (MQ=255)

                                                                                               cTTCCAGCACAACTTCCGTTCCCGGCGTGGAGCGGGAAACCGAACGCAGCGAACAGGATAACCAGCTAAACAGTTCACGGAATTGTAATCCTTGCAGAGGCAAAGGCTGACGAACGCTAATTTGTGCCAGCGTCTTCATATCCGCCCCCt                                                        <  1:268205/150‑1 (MQ=255)

                                                                                                ttCCAGCACAACTTCCGTTCCCGGCGTGGAGCGGGAAACCGAACGCAGCGAACTGGATAACCAGCTAAACAGTTCACGGAATTGTAATCCTTGCAGAGGCAAAGGCTGACGAACGCTAATTTGTGCCAGCGTCTTCATATCCGCCCCCTg                                                       <  2:276734/150‑1 (MQ=255)

                                                                                                      cacaACTTCCGTTCCCGGCGTGGAGCGGGAAACCGAACGCAGCGAACAGGATAACCAGCTAAACAGTTCACGGAATTGTAATCCTTGCAGAGGCAAAGGCTGACGAACGCTAATTTGTGCCAGCGTCTTCATATCCGCCCCCTGAACAcc                                                 <  1:2540181/150‑1 (MQ=255)

                                                                                                      cacaACTTCCGTTCCCGGCGTGGAGCGGGAAACCGAACGCAGCGAACAGGATAACCAGCTAAACAGTTCACGGAATTGTAATCCTTGCAGAGGCAAAGGCTGACGAACGCTAATTTGTGCCAGCGTCTTCATATCCGCCCCCTGAACAcc                                                 <  1:2370169/150‑1 (MQ=255)

                                                                                                      cacaACTTCCGTTCCCGGCGTGGAGCGGGAAACCGAACGCAGCGAACAGGATAACCAGCTAAACAGTTCACGGAATTGTAATCCTTGCAGAGGCAAAGGCTGACGAACGCTAATTTGTGCCAGCGTCTTCATATCCGCCCCCTGAACAcc                                                 <  1:4913214/150‑1 (MQ=255)

                                                                                                      cacaACTTCCGGTCCCGGCGTGGAGCGGGAAACCGAACGCAGCGAACTGGATAACCAGCTAAACAGTTCACGGAATTGTAATCCTTGCAGAGGCAAAGGCTGACGAACGCTAATTTGTGCCAGCGTCTTCATATCCGCCCCCTGAACAcc                                                 <  1:2898573/150‑1 (MQ=255)

                                                                                                        cttcTTCCTTTCCCGGCGTGGAGCGGGAAACCGAACGCAGCGAACTGTATAACCAGCTAAACAGTTCACGGAATTGTTATCCTTGAAGAGGCAAAGTCTGACGAACTCTTATTTGTGCCAGAGTCTTCATATCCGCCCCCTGAACACCGa                                               <  1:732997/147‑1 (MQ=255)

                                                                                                         aaCTTCCGTTCCCGGCGTGGAGCGGGAAACCGAACGCAGCGAACTGGATAACCAGCTAAACAGTTCACGGAATTGTAATCCTTGCAGAGGCAAAGGCTGACGAACGCTAATTTGTGCCAGCGTCTTCATATCCGCCCCCTGAACACCGAt                                              <  1:1451927/150‑1 (MQ=255)

                                                                                                         aaCTTCCGTTCCCGGCGTGGAGCGGGAAACCGAACGCAGCGAACTGGATAACCAGCTAAACAGTTCACGGAATTGTAATCCTTGCAGAGGCAAAGGCTGACGAACGCTAATTTGTGCCAGCGTCTTCATATCCGCCCCCTGAACACCGAt                                              <  1:4698990/150‑1 (MQ=255)

                                                                                                         aaCTTCCGTTCCCGGCGTGGAGCGGGAAACCGAACGCAGCGAACTGGATAACCAGCTAAACAGTTCACGGAATTGTAATCCTTGCAGAGGCAAAGGCTGACGAACGCTAATGTGTGCCAGCGTCTTCATATCCGCCCCCTGAACACCGAt                                              >  2:2141707/1‑150 (MQ=255)

                                                                                                          acgtCCGTTCCCGGCGTGGAGCGGGAAACCGAACGCAGCGAACTGGATAACCAGCTAAACAGTTCACGGAATTGTAATCCTTGCAGAGGCAAAGGCTGACGAACGCTAATTTGTGCCAGCGTCTTCATATCCGCCCCCTGAACACCGATg                                             <  1:4837864/147‑1 (MQ=255)

                                                                                                           cTTCCGTTCCCGGCGTGGAGCGGGAAACCGAACGCAGCGAACTGGATAACCAGCTAAACAGTTCACGGAATTGTAATCCTTGCAGAGGCAAAGGCTGACGAACGCTAATTTGTGCCAGCGTCTTCATATCCGCCCCCTGAACACCGATgg                                            <  1:1000204/150‑1 (MQ=255)

                                                                                                           cTTCCGTTCCCGGCGTGGAGCGGGAAACCGAACGCAGCGAACTGGATAACCAGCTAAACAGTTCACGGAATTGTAATCCTTGCAGAGGCAAAGGCTGACGAACGCTAATTTGTGCCAGCGTCTTCATATCCGCCCCCTGAACACCGATgg                                            <  2:303815/150‑1 (MQ=255)

                                                                                                            ttCCGTTCCCGGCGTGGAGCGGGAAACCGAACGCAGCGAACTGGATAACCAGCTAAACAGTTCACGGAATTGTAATCCTTGCAGAGGCAAAGGCTGACGAACGCTAATTTGTGCCAGCGTCTTCATATCCGCCCCCTGAACACCGATGGa                                           <  1:3428338/150‑1 (MQ=255)

                                                                                                             tCCGTTCCCGGCGTGGAGCGGGAAACCGAACGCAGCGAACTGGATAACCAGCTAAACAGTTCACGGAATTGTAATCCTTGCAGAGGCAAAGGCTGACGAACGCTAATTTGTGCCAGCGTCTTCATATCCGCCCCCTGAACACCGATGGaa                                          <  2:4033513/150‑1 (MQ=255)

                                                                                                             tCCGTTCCCGGCGTGGAGCGGGAAACCGAACGCAGCGAACAGGATAACCAGCTAAACAGTTCACGGAATTGTAATCCTTGCAGAGGCAAAGGCTGACGAACGCTAATTTGTGCCAGCGTCTTCATATCCGCCCCCTGAACACCGATGGaa                                          <  2:3688344/150‑1 (MQ=255)

                                                                                                               cGTTCCCGGCGTGGAGCGGGAAACCGAACGCAGCGAACAGGATAACCAGCTAAACAGTTCCCGGACTTGTAATCCTTGCAGAGGCAAAGGCTGACGAACGCTAAGTTGTGCCAGCGTCTTCATATCCGCCCCCTGCACCCCGAGGGcaaa                                        >  2:3853124/1‑150 (MQ=255)

                                                                                                                  tCCCGGCGTGGAGCGGGAAACCGAACGCAGCGAACTGGATAACCAGCTAAACAGTTCACGGAATTGTAATCCTTGCAGAGGCAAAGGCTGACGAACGCTAATTTGTGCCAGCGTCTTCATATCCGCCCCCTGAACACCGATGGAaaagaa                                     <  1:4208258/150‑1 (MQ=255)

                                                                                                                   cccGGCGTGGAGCGGGAAACCGAACGCAGCGAACTGGATAACCAGCTAAACAGTTCACGGAATTGTAATCCTTGCAGAGGCAAAGGCTGACGAACGCTAATTTGTGCCAGCGTCTTCATATCCGCCCCCTGAACACCGATGGAaaagaaa                                    <  1:1683190/150‑1 (MQ=255)

                                                                                                                    ccGGCGTGGAGCGGGAAACCGAACGCAGCGAACTGGATAACCAGCTAAACAGTTCACGGAATTGTAATCCTTGCAGAGGCAAAGGCTGACGAACGCTAATTTGTGCCAGCGTCTTCATATCCGCCCCCTGAACACCGATGGAaaagaaag                                   >  1:442951/1‑150 (MQ=255)

                                                                                                                      ggCGTGGAGCGGGAAACCGAACGCAGCGAACTGGATAACCAGCTAAACAGTTCACGGAATTGTAATCCTTGCAGAGGCAAAGGCTGACGAACGCTAATTTGTGCCAGCGTCTTCATATCCGCCCCCTGAACACCGATGGAAAAGAAAGCa                                 >  2:253601/1‑150 (MQ=255)

                                                                                                                        cGTGGAGCGGGAAACCGAACGCAGCGAACTGGATAACCAGCTAAACAGTTCACGGAATTGTAATCCTTGCAGAGGCAAAGGCTGACGAACGCTAATTTGTGCCAGCGTCTTCATATCCGCCCCCTGAACACCGATGGAAAAGAAAGCaaa                               <  2:3945557/150‑1 (MQ=255)

                                                                                                                              gCGGGAAACCGAACGCAGCGAACAGGATAACCAGCTAAACAGTTCACGGAATTGTAATCCTTGCAGAGGCAAAGGCTGACGAACGCTAATTTGTGCCAGCGTCTTCATATCCGCCCCCTGAACACCGATGGAAAAGAAAGCAAACCtttt                         <  1:4622319/150‑1 (MQ=255)

                                                                                                                               cGGGAAACCGAACGCAGCGAACTGGATAACCAGCTAAACAGTTCACGGAATTGTAATCCTTGCAGAGGCAAAGGCTGACGAACGCTAATTTGTGCCAGCGTCTTCATATCCGCCCCCTGAACACCGATGGAAAAGAAAGCAAACCTTTTa                        >  2:154656/1‑150 (MQ=255)

                                                                                                                               cGGGAAACCGAACGCAGCGAACTGGATAACCAGCTAAACAGTTCACGGAATTGTAATCCTTGCAGAGGCAAAGGCTGACGAACGCTAATTTGTGCCAGCGTCTTCATATCCGCCCCCTGAACACCGATGGAAAAGAAAGCAAACCTTTTa                        <  1:2132716/150‑1 (MQ=255)

                                                                                                                                gggAAACCGAACGCAGCGAACTGGATAACCAGCTAAACAGTTCACGGAATTGTAATCCTTGCAGAGGCAAAGGCTGACGAACGCTAATTTGTGCCAGCGTCTTCATATCCGCCCCCTGAACACCGATGGAAAAGAAAGCAAACCTTTTAt                       <  2:743221/150‑1 (MQ=255)

                                                                                                                                gggAAACCGAACGCAGCGAACTGGATAACCAGCTAAACAGTTCACGGAATTGTAATCCTTGCAGAGGCAAAGGCTGACGAACGCTAATTTGTGCCAGCGTCTTCATATCCGCCCCCTGAACACCGATGGAAAAGAAAGCAAACCTTTTAt                       >  1:2048868/1‑150 (MQ=255)

                                                                                                                                gggAAACCGAACGCAGCGAACTGGATAACCAGCTAAACAGTTCACGGAATTGTAATCCTTGCAGAGGCAAAGGCTGACGAACGCTAATTTGTGCCAGCGTCTTCATATCCGCCCCCTGAACACCGATGGAAAAGAAAGCAAACCTTTTAt                       <  2:61070/150‑1 (MQ=255)

                                                                                                                                gggAAACCGAACGCAGCGAACTGGATAACCAGCTAAACAGTTCACGGAATTGTAATCCTTGCAGAGGCAAAGGCTGACGAACGCTAATTTGTGCCAGCGTCTTCATATCCGCCCCCTGAACACCGATGGAAAAGAAAGCAAACCTTTTAt                       >  1:5013647/1‑150 (MQ=255)

                                                                                                                                  gAAACCGAACGCAGCGAACTGGATAACCAGCTAAACAGTTCACGGAATTGTAATCCTTGCAGAGGCAAAGGCTGACGAACGCCAATTTGTGCCAGCGTCTTCATATCCGCCCCCTGAACACCGATGGAAAAGAAAGCAAACCTTTTAtct                     <  2:4606749/150‑1 (MQ=255)

                                                                                                                                   aaaCCGAACGCAGCGAACTGGATAACCAGCTAAACAGTTCACGGAATTGTAATCCTTGCAGAGGCAAAGGCTGACGAACGCTAATTTGTGCCAGCGTCTTCATATCCGCCCCCTGAACACCGATGGAAAAGAAAGCAAACCTTTTAtctt                    <  1:1925354/150‑1 (MQ=255)

                                                                                                                                   aaaCCGAACGCAGCGAACTGGATAACCAGCTAAACAGTTCACGGAATTGTAATCCTTGCAGAGGCAAAGGCTGACGAACGCTAATTTGTGCCAGCGTCTTCATATCCGCCCCCTGAACACCGATGGAAAAGAAAGCAAACCTTTTAtctt                    <  1:5119457/150‑1 (MQ=255)

                                                                                                                                     aCCGAACGCAGCGAACTGGATAACCAGCTAAACAGTTCACGGAATTGTAATCCTTGCAGAGGCAAAGGCTGACGAACGCTAATTTGTGCCAGCGTCTTCATATCCGCCCCCTGAACACCGATGGAAAAGAAAGCAAACCTTTTAtcttct                  <  1:4522096/150‑1 (MQ=255)

                                                                                                                                       cGAACGCAGCGAACTGGATAACCAGCTAAACAGTTCACGGAATTGTAATCCTTGCAGAGGCAAAGGCTGACGAACGCTAATTTGTGCCAGCGTCTTCATATCCGCCCCCTGAACACCGATGGAAAAGAAAGCAAACCTTTTATCttcttc                <  1:2431261/150‑1 (MQ=255)

                                                                                                                                         aaCGCAGCGAACTGGATAACCAGCTAAACAGTTCACGGAATTGTAATCCTTGCAGAGGCAAAGGCTGACGAACGCTAATTTGTGCCAGCGTCTTCATATCCGCCCCCTGAACACCGATGGAAAAGAAAGCAAACCTTTTATCTTCTTccc              >  1:1027931/1‑150 (MQ=255)

                                                                                                                                         aaCGCAGCGAACTGGATAACCAGCTAAACAGTTCACGGAATTGTAATCCTTGCAGAGGCAAAGGCTGACGAACGCTAATTTGTGCCAGCGTCTTCATATCCGCCCCCTGAACACCGATGGAAAAGAAAGCAAACCTTTTATCTTCTTccc              <  1:3024462/150‑1 (MQ=255)

                                                                                                                                           cGCAGCGAACTGGATAACCAGCTAAACAGTTCACGGAATTGTAATCCTTGCAGAGGCAAAGGCTGACGAACGCTAATTTGTGCCAGCGTCTTCATATCCGCCCCCTGAACACCGATGGAAAAGAAAGCAAACCTTTTATCTTCTTccccc            <  2:925255/150‑1 (MQ=255)

                                                                                                                                            gCAGCGAACTGGATAACCAGCTAAACAGTTCACGGAATTGTAATCCTTGCAGAGGCAAAGGCTGACGAACGCTAATTTGTGCCAGCGTCTTCATATCCGCCCCCTGAACACCGATGGAAAAGAAAGCAAACCTTTTATCTTCTTcccccc           >  2:2459971/1‑150 (MQ=255)

                                                                                                                                                cGAACTGGATAACCAGCTAAACAGTTCACGGAATTGTAATCCTTGCAGAGGCAAAGGCTGACGAACGCTAATTTGTGCCAGCGTCTTCATATCCGCCCCCTGAACACCGATGGAAAAGAAAGCAAACCTTTTATCTTCTTCCCCCCGaaa       >  2:1889368/1‑150 (MQ=255)

                                                                                                                                                 gAACTGGATAACCAGCTAAACAGTTCACGGAATTGTAATCCTTGCAGAGGCAAAGGCTGACGAACGCTAATTTGTGCCAGCGTCTTCATATCCGCCCCCTGAACACCGATGGAAAAGAAAGCAAACCTTTTATCTTCTTCCCCCCGAAac      >  2:2021645/1‑150 (MQ=255)

                                                                                                                                                  aaCTGGATAACCAGCTAAACAGTTCACGGAATTGTAATCCTTGCAGAGGCAAAGGCTGACGAACGCTAATTTGTGCCAGCGTCTTCATATCCGCCCCCTGAACACCGATGGAAAAGAAAGCAAACCTTTTATCTTCTTCCCCCCGAAaca     <  1:4650278/150‑1 (MQ=255)

                                                                                                                                                   aCTGGATAACCAGCTAAACAGTTCACGGAATTGTAATCCTTGCAGAGGCAAAGGCTGACGAACGCTAATTTGTGCCAGCGTCTTCATATCCGCCCCCTGAACACCGATGGAAAAGAAAGCAAACCTTTTATCTTCTTCCCCCCGAAacac    <  2:3257459/150‑1 (MQ=255)

                                                                                                                                                   aCTGGATAACCAGCTAAACAGTTCACGGAATTGTAATCCTTGCAGAGGCAAAGGCTGACGAACGCTAATTTGTGCCAGCGTCTTCATATCCGCCCCCTGAACACCGATGGAAAAGAAAGCAAACCTTTTATCTTCTTCCCCCCGAAacac    >  2:3236149/1‑150 (MQ=255)

CATCCTGACAAGGCAAGTCCGCGCTGATGTGCGACGTTCCAACGGTTGATGCATAGACCAGACGCCAGCTCACACTGACGTCCAGCCTTTTGGCGCTTCCAGCACAACTTCCGTTCCCGGCGTGGAGCGGGAAACCGAACGCAGCGAACTGGATAACCAGCTAAACAGTTCACGGAATTGTAATCCTTGCAGAGGCAAAGGCTGACGAACGCTAATTTGTGCCAGCGTCTTCATATCCGCCCCCTGAACACCGATGGAAAAGAAAGCAAACCTTTTATCTTCTTCCCCCCGAAACACTT  >  CP009273/2145881‑2146179

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`