BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	158,287	C→A	94.9%	D78Y (GAC→TAC)	ligT ←	2'‑5' RNA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	158,287	0	C	A	94.9%	1088.5 / 43.6	332	D78Y (GAC→TAC)	ligT	2'‑5' RNA ligase
Reads supporting (aligned to +/- strand): ref base C (8/9); new base A (168/147); total (176/156)
Fisher's exact test for biased strand distribution p-value = 6.28e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.38e-01

Only 100 of 334 total aligned reads displayed.

TAATATGTGGATGAAACGGACGATTGCTTTGAAAACAACCGCTGCGGGCAGCCTGTGAACGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTCATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTCTCGGCGCTCACTTCGCCTAAAAATGCCAGAGTCAGATGCAAATTATCGGCGGCGACCGGACGTCCCGCCTCAGGTGGGA  >  CP009273/158140‑158433

tAATATGTGGATGAAACGGACGATTGCTTTGAAAACAACCGCTGCGGGCAGCCTGTGAACGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGtaat                                                                                                                                                  >  2:4714261/1‑150 (MQ=255)

  atatGTGGATGAAACGGACGATTGCTTTGAAAACAACCGCTGCGGGCAGCCTGTGAACGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGtaatca                                                                                                                                                >  2:4923778/1‑150 (MQ=255)

    atGTGGATGAAACGGACGATTGCTTTGAAAACAACCGCTGCGGGCAGCCTGTGAACGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAg                                                                                                                                              <  2:4835666/150‑1 (MQ=255)

      gtgGATGAAACGGACGATTGCTTTGAAAACAACCGCTGCGGGCAGCCTGTGAACGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCg                                                                                                                                            <  1:3175855/150‑1 (MQ=255)

        ggATGAAACGGACGATTGCTTTGAAAACAACCGCTGCGGGCAGCCTGTGAACGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTCATCAAGCGTg                                                                                                                                          >  2:4368371/1‑150 (MQ=255)

         gATGAAACGGACGATTGCTTTGAAAACAACCGCTGCGGGCAGCCTGTGAACGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGa                                                                                                                                         >  1:382521/1‑150 (MQ=255)

            gAAACGGACGATTGCTTTGAAAACAACCGCTGCGGGCAGCCTGTGAACGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAgtg                                                                                                                                      >  2:1744623/1‑150 (MQ=255)

             aaaCGGACGATTGCTTTGAAAACAACCGCTGCGGGCAGCCTGTGAACGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAgtgt                                                                                                                                     <  2:1924365/150‑1 (MQ=255)

                 ggACGATTGCTTTGAAAACAACCGCTGCGGGCAGCCTGTGAACGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGaaa                                                                                                                                 <  1:5104876/150‑1 (MQ=255)

                 ggACGATTGCTTTGAAAACAACCGCTGCGGGCAGCCTGTGAACGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGaaa                                                                                                                                 >  1:4898889/1‑150 (MQ=255)

                   aCGATTGCTTTGAAAACAACCGCTGCGGGCAGCCTGTGAACGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAAcc                                                                                                                               <  1:1381567/150‑1 (MQ=255)

                     gATTGCTTTGAAAACAACCGCTGCGGGCAGCCTGTGAACGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAg                                                                                                                             <  1:4121290/150‑1 (MQ=255)

                     gATTGCTTTGAAAACAACCGCTGCGGGCAGCCTGTGAACGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAg                                                                                                                             <  2:2667420/150‑1 (MQ=255)

                     gATTGCTTTGAAAACAACCGCTGCGGGCAGCCTGTGAACGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAg                                                                                                                             >  1:1119698/1‑150 (MQ=255)

                      aTTGCTTTGAAAACAACCGCTGCGGGCAGCCTGTGAACGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAgg                                                                                                                            >  1:168668/1‑150 (MQ=255)

                      aTTGCTTTGAAAACAACCGCTGCGGGCAGCCTGTGAACGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAgg                                                                                                                            >  2:3796141/1‑150 (MQ=255)

                        tGCTTTGAAAACAACCGCTGCGGGCAGCCTGTGAACGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAACCCAGGtt                                                                                                                          >  1:3223313/1‑150 (MQ=255)

                         gCTTTGAAAACAACCGCTGCGGGCAGCCTGTGAACGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTg                                                                                                                         >  2:2301321/1‑150 (MQ=255)

                           tttGAAAACAACCGCTGCGGGCAGCCTGTGAACGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGAc                                                                                                                       >  2:3948053/1‑150 (MQ=255)

                            ttGAAAACAACCGCTGCGGGCAGCCTGTGAACGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACg                                                                                                                      >  2:2796367/1‑150 (MQ=255)

                            ttGAAAACAACCGCTGCGGGCAGCCTGTGAACGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACg                                                                                                                      <  2:1891787/150‑1 (MQ=255)

                             tGAAAACAACCGCTGCGGGCAGCCTGTGAACGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGa                                                                                                                     >  2:3737005/1‑150 (MQ=255)

                                   caacCGCTGCGGGCAGCCTGTGAACGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAAtccgt                                                                                                               >  2:2187532/1‑150 (MQ=255)

                                   caacCGCTGCGGGCAGCCTGTGAACGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAAtccgt                                                                                                               <  1:3107288/150‑1 (MQ=255)

                                   caacCGCTGCGGGCAGCCTGTGAACGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAAtccgt                                                                                                               >  2:3606907/1‑150 (MQ=255)

                                   caacCGCTGCGGGCAGCCTGTGAACGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAAtccgt                                                                                                               >  1:2624903/1‑150 (MQ=255)

                                    aacCGCTGCGGGCAGCCTGTGAACGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAAtccgtc                                                                                                              >  1:5007900/1‑150 (MQ=255)

                                    aacCGCTGCGGGCAGACTGAGAACGGAGCATATTCGCCAGCTGGATAAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAATCGTGAGTGTGAAACCAGGATGACGAAtccgtc                                                                                                              >  2:823499/1‑150 (MQ=255)

                                         cTGCGGGCAGCCTGTGAACGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCt                                                                                                         <  2:1564653/150‑1 (MQ=255)

                                         cTGCGGGCAGCCTGTGAACGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCt                                                                                                         >  2:1534995/1‑150 (MQ=255)

                                            cGGGCAGCCTGTGAACGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTaaa                                                                                                      <  1:4233027/150‑1 (MQ=255)

                                             gggCAGCCTGTGAACGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTaaaa                                                                                                     <  1:2722489/150‑1 (MQ=255)

                                             gggCAGCCTGTGAACGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTaaaa                                                                                                     >  1:4819312/1‑150 (MQ=255)

                                             gggCAGCCTGTGAACGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTaaaa                                                                                                     <  2:4054649/150‑1 (MQ=255)

                                              ggCAGCCTGTGAACGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAag                                                                                                    >  2:323724/1‑150 (MQ=255)

                                                 aGCCTGTGAACGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAgaga                                                                                                 >  1:4539641/1‑150 (MQ=255)

                                                 aGCCTGTGAACGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAgaga                                                                                                 <  2:3497998/150‑1 (MQ=255)

                                                   ccTGTGAACGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTCATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGaaa                                                                                               <  1:3771693/150‑1 (MQ=255)

                                                    cTGTGAACGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAg                                                                                              >  2:1963769/1‑150 (MQ=255)

                                                      gtgAACGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAgcg                                                                                            >  1:4021824/1‑150 (MQ=255)

                                                      gtgAACGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAgcg                                                                                            <  1:2296962/150‑1 (MQ=255)

                                                      gtgAACGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAgcg                                                                                            <  1:2011568/150‑1 (MQ=255)

                                                      gtgAACGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAgcg                                                                                            >  2:91992/1‑150 (MQ=255)

                                                        gAACGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGcc                                                                                          <  2:1280998/150‑1 (MQ=255)

                                                          aCGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTCATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCtt                                                                                        <  1:2243319/150‑1 (MQ=255)

                                                           cGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTtc                                                                                       >  1:472331/1‑150 (MQ=255)

                                                              aGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCATCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGATAAAGCGCCTTctct                                                                                    <  2:3403109/150‑1 (MQ=255)

                                                                 atatTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTtc                                                                                 >  2:1641542/1‑150 (MQ=255)

                                                                 atatTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTtc                                                                                 <  2:2918152/150‑1 (MQ=255)

                                                                 atatTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTtc                                                                                 >  1:2103875/1‑150 (MQ=255)

                                                                  tatTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTtct                                                                                >  2:4436723/1‑150 (MQ=255)

                                                                  tatTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTtct                                                                                >  1:4212796/1‑150 (MQ=255)

                                                                    ttCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTCTCg                                                                              <  2:1809615/150‑1 (MQ=255)

                                                                    ttCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTCTCg                                                                              <  2:298925/150‑1 (MQ=255)

                                                                         cAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTCTCGGCGCt                                                                         >  2:354255/1‑150 (MQ=255)

                                                                             tGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTCTCGGCGCTCACt                                                                     >  1:959430/1‑150 (MQ=255)

                                                                             tGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTCTCGGCGCTCACt                                                                     <  2:1382081/150‑1 (MQ=255)

                                                                               gATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTCTCGGCGCTCACTTc                                                                   >  2:1131311/1‑150 (MQ=255)

                                                                                aTTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTCTCGGCGCTCACTTCg                                                                  <  2:2613264/150‑1 (MQ=255)

                                                                                   aaGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTCTCGGCGCTCACTTCGCCt                                                               <  1:4429122/150‑1 (MQ=255)

                                                                                    aGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTtc                                                                                 <  1:851568/131‑1 (MQ=255)

                                                                                     gCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTCTCGGCGCTCACTTCGCCTaa                                                             >  1:1997198/1‑150 (MQ=255)

                                                                                            ggcggcTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTCTCGGCGCTCACTTCGCCTAAAAATGcc                                                      >  1:4486179/1‑150 (MQ=255)

                                                                                             gcggcTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTCTCGGCGCTCACTTCGCCTAAAAATGCCa                                                     >  2:1841556/1‑150 (MQ=255)

                                                                                              cggcTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTCTCGGCGCTCACTTCGCCTAAAAATGCCag                                                    >  1:1023184/1‑150 (MQ=255)

                                                                                              cggcTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTCTCGGCGCTCACTTCGCCTAAAAATGCCag                                                    <  2:2126095/150‑1 (MQ=255)

                                                                                              cggcTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTCTCGGCGCTCACTTCGCCTAAAAATGCCag                                                    >  1:1544959/1‑150 (MQ=255)

                                                                                                 cTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTCTCGGCGCTCACTTCGCCTAAAAATGCCAGAGt                                                 <  1:3469186/150‑1 (MQ=255)

                                                                                                  tGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTCTCGGCGCTCACTTCGCCTAAAAATGCCAGAGTc                                                >  1:1624037/1‑150 (MQ=255)

                                                                                                  tGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTCTCGGCGCTCACTTCGCCTAAAAATGCCAGAGTc                                                >  1:4033329/1‑150 (MQ=255)

                                                                                                      gCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTCTCGGCGCTCACTTCGCCTAAAAATGCCAGAGTCAGAt                                            >  2:4118856/1‑150 (MQ=255)

                                                                                                      gCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTCTCGGCGCTCACTTCGCCTAAAAATGCCAGAGTCAGAt                                            <  1:4576309/150‑1 (MQ=255)

                                                                                                         tCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTCTCGGCGCTCACTTCGCCTAAAAATGCCAGAGTCAGATGCa                                         >  2:1742274/1‑150 (MQ=255)

                                                                                                            cTAACCACACCACACGCGAACGCAGCCTTTGTCGGGCGTAATCAAGCGTGTGTGTTAAACCTGGTTGACGAATCCGTCGGGATAAAAGAGAACGCGCCTTCTCTTTCTCGGCGCTCACTTCGCCTAAAAATGCCAGTGTCTGATGCAAAt                                      <  2:4329073/150‑1 (MQ=255)

                                                                                                               accacaccacACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTCTCGGCGCTCACTTCGCCTAAAAATGCCAGAGTCAGATGCAAATTAt                                   <  1:3903022/150‑1 (MQ=255)

                                                                                                                ccacaccacaCGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTCTCGGCGCTCACTTCGCCTAAAAATGCCAGAGTCAGATGCAAATTATc                                  >  1:1249571/1‑150 (MQ=255)

                                                                                                                ccacaccacaCGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTCTCGGCGCTCACTTCGCCTAAAAATGCCAGAGTCAGATGCAAATTATc                                  <  1:2810601/150‑1 (MQ=255)

                                                                                                                 cacaccacacGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTCTCGGCGCTCACTTCGCCTAAAAATGCCAGAGTCAGATGCAAATTATcg                                 >  1:911985/1‑150 (MQ=255)

                                                                                                                   caccacacGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTCTCGGCGCTCACTTCGCCTAAAAATGCCAGAGTCAGATGCAAATTATcggc                               <  1:601252/150‑1 (MQ=255)

                                                                                                                   caccacacGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTCTCGGCGCTCACTGCGCCTAAAAATGCCAGAGGCAGATGCACAGTATcggc                               >  2:3921867/1‑150 (MQ=255)

                                                                                                                    accacacGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTCTCGGCGCTCACTTCGCCTAAAAATGCCAGAGTCAGATGCAAATTATcggcg                              >  2:4247874/1‑150 (MQ=255)

                                                                                                                      cacacGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTCTCGGCGCTCACTTCGCCTAAAAATGCCAGAGTCAGATGCAAATTATCggcggc                            >  1:88853/1‑150 (MQ=255)

                                                                                                                       acacGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTCTCGGCGCTCACTTCGCCTAAAAATGCCAGAGTCAGATGCAAAGTATCGgcggcg                           >  2:3328421/1‑150 (MQ=255)

                                                                                                                        cacGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTCTCGGCGCTCACTTCGCCTAAAAATGCCAGAGTCAGATGCAAATTATCGGCGGCGa                          <  1:2116300/150‑1 (MQ=255)

                                                                                                                         acGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTCTCGGCGCTCACTTCGCCTAAAAATGCCAGAGTCAGATGCAAATTATCGGCGGCGAc                         <  2:453651/150‑1 (MQ=255)

                                                                                                                          cgcgAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTCTCGGCGCTCACTTCGCCTAAAAATGCCAGAGTCAGATGCAAATTATCGGCGGCGAcc                        <  1:65861/150‑1 (MQ=255)

                                                                                                                            cgAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTCTCGGCGCTCACTTCGCCTAAAAATGCCAGAGTCAGATGCAAATTATCGGCGGCGACCgg                      <  2:611464/150‑1 (MQ=255)

                                                                                                                            cgAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTCTCGGCGCTCACTTCGCCTAAAAATGCCAGAGTCAGATGCAAATTATCGGCGGCGACCgg                      <  2:5010166/150‑1 (MQ=255)

                                                                                                                            cgAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTCTCGGCGCTCACTTCGCCTAAAAATGCCAGAGTCAGATGCAAATTATCGGCGGCGACCgg                      <  1:3742221/150‑1 (MQ=255)

                                                                                                                                cGCAGCCATTGTCCGGCGTCATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTCTCGGCGCTCACTTCGCCTAAAAATGCCAGAGTCAGATGCAAATTATCGGCGGCGACCGGACGt                  <  1:1060684/150‑1 (MQ=255)

                                                                                                                                      cATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTCTCGGCGCTCACTTCGCCTAAAAATGCCAGAGTCAGATGCAAATTATCGGCGGCGACCGGACGTCCCGcc            >  2:673373/1‑150 (MQ=255)

                                                                                                                                       aTTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTCTCGGCGCTCACTTCGCCTAAAAATGCCAGAGTCAGATGCAAATTATCGGCGGCGACCGGACGTCCCGCCt           >  1:1753798/1‑150 (MQ=255)

                                                                                                                                        ttGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTCTCGGCGCTCACTTCGCCTAAAAATGCCAGAGTCAGATGCAAATTATCGGCGGCGACCGGACGTCCCGCCTc          <  2:2399272/150‑1 (MQ=255)

                                                                                                                                        ttGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTCTCGGCGCTCACTTCGCCTAAAAATGCCAGAGTCAGATGCAAATTATCGGCGGCGACCGGACGTCCCGCCTc          >  2:974686/1‑150 (MQ=255)

                                                                                                                                         tGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTCTCGGCGCTCACTTCGCCTAAAAATGCCAGAGTCAGATGCAAATTATCGGCGGCGACCGGACGTCCCGCCTCa         <  1:1945411/150‑1 (MQ=255)

                                                                                                                                          gTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTCTCGGCGCTCACTTCGCCTAAAAATGCCAGAGTCAGATGCAAATTATCGGCGGCGACCGGACGTCCCGCCTCAg        >  1:337029/1‑150 (MQ=255)

                                                                                                                                          gTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTCTCGGCGCTCACTTCGCCTAAAAATGCCAGAGTCAGATGCAAATTATCGGCGGCGACCGGACGTCCCGCCTCAg        >  1:2932416/1‑150 (MQ=255)

                                                                                                                                               gCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTCTCGGCGCTCACTTCGCCTAAAAATGCCAGAGTCAGATGCAAATTATCGGCGGCGACCGGACGTCCCGCCTCAGGTggg   <  2:2697245/150‑1 (MQ=255)

                                                                                                                                                cGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTCTCGGCGCTCACTTCGCCTAAAAATGCCAGAGTCAGATGCAAATTATCGGCGGCGACCGGACGTCCCGCCTCAGGTGGGa  <  1:3649484/150‑1 (MQ=255)

                                                                                                                                                cGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTCTCGGCGCTCACTTCGCCTAAAAATGCCAGAGTCAGATGCAAATTATCGGCGGCGACCGGACGTCCCGCCTCAGGTGGGa  <  1:2429749/150‑1 (MQ=255)

TAATATGTGGATGAAACGGACGATTGCTTTGAAAACAACCGCTGCGGGCAGCCTGTGAACGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTCATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTCTCGGCGCTCACTTCGCCTAAAAATGCCAGAGTCAGATGCAAATTATCGGCGGCGACCGGACGTCCCGCCTCAGGTGGGA  >  CP009273/158140‑158433

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`