Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,353,889 C→A 26.5% V335V (GTG→GTT)  rhmD ← L‑rhamnonate dehydratase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,353,8890CA26.5% 602.8 / 193.1 270V335V (GTG→GTT) rhmDL‑rhamnonate dehydratase
Reads supporting (aligned to +/- strand):  ref base C (104/94);  new base A (40/32);  total (144/126)
Fisher's exact test for biased strand distribution p-value = 6.81e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.85e-01

Only 100 of 271 total aligned reads displayed.

AACCGGGTTTATCAAGCACTGATTTATGAATACGACCATTCACCGGAACCGGCTCATTGAGCAGAATCGGGTCAAACTGCGGACGCATCGTTGAACAATCCGGGCTGGTCATCAGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATCACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCGGCAATTTCCACCAGCGTGGTTAAACCACCGCACCAGCCAACATCCGGCTGCATAATGTCGATACCGGTTTCTGAAAGCGT  >  CP009273/2353741‑2354038
                                                                                                                                                    |                                                                                                                                                     
  ccGGGTTTATCAAGCACTGATTTATGAATACGACCATTCACCGGAACCGGCTCATTGAGCAGAATCGGGTCAAACTGCGGACGCATCGTTGAACAATCCGGGCTGGTCATCAGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATCAcc                                                                                                                                                    >  1:199719/1‑150 (MQ=255)
  ccGGGTTTATCAAGCACTGATTTATGAATACGACCATTCACCGGAACCGGCTCATTGAGCAGAATCGGGTCAAACTGCGGACGCATCGTTGAACAATCCGGGCTGGTCATCAGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATAAcc                                                                                                                                                    <  2:628105/150‑1 (MQ=255)
  ccGGGTTTATCAAGCACTGATTTATGAATACGACCATTCACCGGAACCGGCTCATTGAGCAGAATCGGGTCAAACTGCGGACGCATCGTTGAACAATCCGGGCTGGTCATCAGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATAAcc                                                                                                                                                    >  1:201802/1‑150 (MQ=255)
    gggTTTATCAAGCACTGATTTATGAATACGACCATTCACCGGAACCGGCTCATTGAGCAGAATCGGGTCAAACTGCGGACGCATCGTTGAACAATCCGGGCTGGTCATCAGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATCACCGc                                                                                                                                                  >  1:4453786/1‑150 (MQ=255)
    gggTTTATCAAGCACTGATTTATGAATACGACCATTCACCGGAACCGGCTCATTGAGCAGAATCGGGTCAAACTGCGGACGCATCGTTGAACAATCCGGGCTGGTCATCAGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATAACCGc                                                                                                                                                  >  1:2916187/1‑150 (MQ=255)
     ggTTTATCAAGCACTGATTTATGAATACGACCATTCACCGGAACCGGCTCATTGAGCAGAATCGGGTCAAACTGCGGACGCATCGTTGAACAATCCGGGCTGGTCATCAGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATCACCGCa                                                                                                                                                 >  2:1122701/1‑150 (MQ=255)
      gTTTATCAAGCACTGATTTATGAATACGACCATTCACCGGAACCGGCTCATTGAGCAGAATCGGGTCAAACTGCGGACGCATCGTTGAACAATCCGGGCTGGTCATCAGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATCACCGCAt                                                                                                                                                <  1:2675496/150‑1 (MQ=255)
        ttATCAAGCACTGATTTATGAATACGACCATTCACCGGAACCGGCTCATTGAGCAGAATCGGGTCAAACTGCGGACGCATCGTTGAACAATCCGGGCTGGTCATCAGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATAACCGCATgg                                                                                                                                              >  2:4726234/1‑150 (MQ=255)
         tATCAAGCACTGATTTATGAATACGACCATTCACCGGAACCGGCTCATTGAGCAGAATCGGGTCAAACTGCGGACGCATCGTTGAACAATCCGGGCTGGTCATCAGGAATTCGCTGAATGGCGTATTGGTGACGGTGATCACCGCATGGt                                                                                                                                             >  1:4851841/1‑150 (MQ=255)
            cAAGCACTGATTTATGAATACGACCATTCACCGGAACCGGCTCATTGAGCAGAATCGGGTCAAACTGCGGACGCATCGTTGAACAATCCGGGCTGGTCATCAGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATCACCGCATGGTgag                                                                                                                                          <  1:4361123/150‑1 (MQ=255)
               gCACTGATTTATGAATACGACCATTCACCGGAACCGGCTCATTGAGCAGAATCGGGTCAAACTGCGGACGCATCGTTGAACAATCCGGGCTGGTCATCAGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATCACCGCATGGTGAGAGt                                                                                                                                       >  2:2312710/1‑150 (MQ=255)
                cACTGATTTATGAATACGACCATTCACCGGAACCGGCTCATTGAGCAGAAACGGGTCAAACTGCGGACGCATCGTTGAACAATCCGTGCTGGTCATCATTAATTCGCTGAATGGCGTATTGGTTAAGGTGATAACCGCATGGTGAGAGTa                                                                                                                                      >  1:2224821/1‑150 (MQ=255)
                 aCTGATTTATGAATACGACCATTCACCGGAACCGGCTCATTGAGCAGAATCGGGTCAAACTGCGGACGCATCGTTGAACAATCCGGGCTGGTCATCAGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATAACCGCATGGTGAGAGTaa                                                                                                                                     >  2:890160/1‑150 (MQ=255)
                    gATTTATGAATACGACCATTCACCGGAACCGGCTCATTGAGCAGAATCGGGTCAAACTGCGGACGCATCGTTGAACAATCCGGGCTGGTCATCAGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATAACCGCATGGTGAGAGTAAACa                                                                                                                                  <  2:1776129/150‑1 (MQ=255)
                    gATTTATGAATACGACCATTCACCGGAACCGGCTCATTGAGCAGAATCGGGTCAAACTGCGGACGCATCGTTGAACAATCCGGGCTGGTCATCAGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATAACCGCATGGTGAGAGTAAACa                                                                                                                                  <  2:4927912/150‑1 (MQ=255)
                     aTTTATGAATACGACCATTCACCGGAACCGGCTCATTGAGCAGAATCGGGTCAAACTGCGGACGCATCGTTGAACAATCCGGGCTGGTCATCAGGAATTCGCTGAATGGCGTATTGGTGACGGTGATCACCGCATGGTGAGAGTAAACAg                                                                                                                                 >  1:5136182/1‑150 (MQ=255)
                      tttATGAATACGACCATTCACCGGAACCGGCTCATTGAGCAGAATCGGGTCAAACTGCGGACGCATCGTTGAACAATCCGGGCTGGTCATCAGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATCACCGCATGGTGAGAGTAAACAGa                                                                                                                                <  2:2880489/150‑1 (MQ=255)
                        tATGAATACGACCATTCACCGGAACCGGCTCATTGAGCAGAATCGGGTCAAACTGCGGACGCATCGTTGAACAATCCGGGCTGGTCATCAGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATAACCGCATGGTGAGAGTAAACAGACg                                                                                                                              <  1:3149040/150‑1 (MQ=255)
                           gAATACGACCATTCACCGGAACCGGCTCATTGAGCAGAATCGGGTCAAACTGCGGACGCATCGTTGAACAATCCGGGCTGGTCATCAGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATCACCGCATGGTGAGAGTAAACAGACGAAc                                                                                                                           >  1:1703875/1‑150 (MQ=255)
                            aaTACGACCAGTCACCGGAACCGGCTCATTGAGCAGAATCGGGTCAAACTGCGGACGCATCGTTGAACAATCCGGGCTGGTCATCAGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATCACCGCATGGTGAGAGTAAACAGACGAAcc                                                                                                                          <  1:4023787/150‑1 (MQ=255)
                             aTACGACCATTCACCGGAACCGGCTCATTGAGCAGAATCGGGTCAAACTGCGGACGCATCGTTGAACAATCCGGGCTGGTCATCAGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATCACCGCATGGTGAGAGTAAACAGACGAACCg                                                                                                                         <  2:3366398/150‑1 (MQ=255)
                                 gACCATTCACCGGAACCGGCTCATTGAGCAGAATCGGGTCAAACTGCGGACGCATCGTTGAACAATCCGGGCTGGTCATCAGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATCACCGCATGGTGAGAGTAAACAGACGAACCGTGCg                                                                                                                     <  2:2123685/150‑1 (MQ=255)
                                 gACCATTCACCGGAACCGGCTCATTGAGCAGAATCGGGTCAAACTGCGGACGCATCGTTGAACAATCCGGGCTGGTCATCAGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATCACCGCATGGGGAGAGTAAACAGACGAACCGTGCg                                                                                                                     >  1:1393687/1‑150 (MQ=255)
                                  aCCATTCACCGGAACCGGCTCATTGAGCAGAATCGGGTCAAACTGCGGACGCATCGTTGAACAATCCGGGCTGGTCATCAGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATCACCGCATGGTGAGAGTAAACAGACGAACCGTGCgg                                                                                                                    >  2:971418/1‑150 (MQ=255)
                                  aCCATTCACCGGAACCGGCTCATTGAGCAGAATCGGGTCAAACTGCGGACGCATCGTTGAACAATCCGGGCTGGTCATCAGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATAACCGCATGGTGAGAGTAAACAGACGAACCGTGCgg                                                                                                                    <  1:2414580/150‑1 (MQ=255)
                                         aCCGGAACCGGCTCATTGAGCAGAATCGGGTCAAACTGCGGACGCATCGTTGAACAATCCGGGCTGGTCATCAGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATCACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCaccacc                                                                                                             >  1:2127189/1‑150 (MQ=255)
                                         aCCGGAACCGGCTCATTGAGCAGAATCGGGTCAAACTGCGGACGCATCGTTGAACAATCCGGGCTGGTCATCAGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATCACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCaccacc                                                                                                             <  1:378949/150‑1 (MQ=255)
                                           cGGAACCGGCTCATTGAGCAGAATCGGGTCAAACTGCGGACGCATCGTTGAACAATCCGGGCTGGTCATCAGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATCACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAg                                                                                                           >  1:1741375/1‑150 (MQ=255)
                                             gAACCGGCTCATTGAGCAGAATCGGGTCAAACTGCGGACGCATCGTTGAACAATCCGGGCTGGTCATCAGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATCACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGtt                                                                                                         >  1:4654434/1‑150 (MQ=255)
                                                 cGGCTCATTGAGCAGAATCGGGTCAAACTGCGGACGCATCGTTGAACAATCCGGGCTGGTCATCAGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATCACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGccc                                                                                                     >  2:5109758/1‑150 (MQ=255)
                                                 cGGCTCATTGAGCAGAATCGGGTCAAACTGCGGACGCATCGTTGAACAATCCGGGCTGGTCATCAGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATAACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGccc                                                                                                     >  1:3307973/1‑150 (MQ=255)
                                                 cGGCTCATTGAGCAGAATCGGGTCAAACTGCGGACGCATCGTTGAACAATCCGGGCTGGTCATCAGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATAACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGccc                                                                                                     >  1:4371476/1‑150 (MQ=255)
                                                   gCTCATTGAGCAGAATCGGGTCAAACTGCGGACGCATCGTTGAACAATCCGGGCTGGTCATCAGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATAACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGccccc                                                                                                   <  1:4285298/150‑1 (MQ=255)
                                                    cTCATTGAGCAGAATCGGGTCAAACTGCGGACGCATCGTTGAACAATCCGGGCTGGTCATCAGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATCACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCt                                                                                                  >  2:4528325/1‑150 (MQ=255)
                                                         tGAGCAGAATCGGGTCAAACTGCGGACGCATCGTTGAACAATCCGGGCTGGTCATCAGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATAACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGAtt                                                                                             <  1:4726234/150‑1 (MQ=255)
                                                           aGCAGAATCGGGTCAAACTGCGGACGCATCGTTGAACAATCCGGGCTGGTCATCAGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATCACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTg                                                                                           >  1:4245101/1‑150 (MQ=255)
                                                               gAATCGGGTCAAACTGCGGACGCATCGTTGAACAATCCGGGCTGGTCATCAGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATCACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGa                                                                                       <  1:123175/150‑1 (MQ=255)
                                                               gAATCGGGTCAAACTGCGGACGCATCGTTGAACAATCCGGGCTGGTCATCAGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATAACCGCAAGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGa                                                                                       >  1:3379529/1‑150 (MQ=255)
                                                                    gggTCAAACTGCGGACGCATCGTTGAACAATCCGGGCTGGTCATCAGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATCACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCg                                                                                  >  1:692229/1‑150 (MQ=255)
                                                                      gTCAAACTGCGGACGCATCGTTGAACAATCCGGGCTGGTCATCAGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATCACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCGGc                                                                                <  1:1378806/150‑1 (MQ=255)
                                                                        cAAACTGCGGACGCATCGTTGAACAATCCGGGCTGGTCATCAGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATCACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCGGCaa                                                                              >  1:3512947/1‑150 (MQ=255)
                                                                         aaaCTGCGGACGCATCGTTGAACAATCCGGGCTGGTCATCAGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATCACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCGGCAAt                                                                             >  2:4243672/1‑150 (MQ=255)
                                                                         aaaCTGCGGACGCATCGTTGAACAATCCGGGCTGGTCATCAGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATCACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCGGCAAt                                                                             >  1:1974426/1‑150 (MQ=255)
                                                                         aaaCTGCGGACGCATCGTTGAACAATCCGGGCTGGTCATCAGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATCACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCGGCAAt                                                                             >  2:124686/1‑150 (MQ=255)
                                                                         aaaCTGCGGACGCATCGTTGAACAATCCGGGCTGGTCATCAGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATCACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCGGCAAt                                                                             >  1:300394/1‑150 (MQ=255)
                                                                           aCTGCGGACGCATCGTTGAACAATCCGGGCTGGTCATCAGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATCACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCGGCAAttt                                                                           <  1:4998451/150‑1 (MQ=255)
                                                                               cGGACGCATCGTTGAACAATCCGGGCTGGTCATCAGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATCACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCGGCAATTTccac                                                                       >  1:392564/1‑150 (MQ=255)
                                                                               cGGACGCATCGTTGAACAATCCGGGCTGGTCATCAGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATCACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCGGCAATTTccac                                                                       >  1:2893092/1‑150 (MQ=255)
                                                                                 gACGCATCGTTGAACAATCCGGGCTGGTCATCAGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATCACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCGGCAATTTccacca                                                                     >  1:4108474/1‑150 (MQ=255)
                                                                                 gACGCATCGTTGAACAATCCGGGCTGGTCATCAGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATAACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCGGCAATTTccacca                                                                     >  2:839955/1‑150 (MQ=255)
                                                                                   cGCATCGTTGAACAATCCGGGCTGGTCATCAGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATCACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCGGCAATTTCCACCAGc                                                                   >  1:851181/1‑150 (MQ=255)
                                                                                    gCATCGTTGAACAATCCGGGCTGGTCATCAGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATCACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCGGCAATTTCCACCAGCg                                                                  <  1:1053487/150‑1 (MQ=255)
                                                                                    gCATCGTTGAACAATCCGGGCTGGTCATCAGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATCACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCGGCAATTTCCACCAGCg                                                                  >  2:1629418/1‑150 (MQ=255)
                                                                                      aTCGTTGAACAATCCGGGCTGGTCATCAGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATCACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCGGCAATTTCCACCAGCGTg                                                                >  2:1730695/1‑150 (MQ=255)
                                                                                         gTTGAACAATCCGGGCTGGTCATCAGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATCACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCGGCAATTTCCACCAGCGTGGtt                                                             <  2:3888625/150‑1 (MQ=255)
                                                                                         gTTGAACAATCCGGGCTGGTCATCAGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATAACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCGGCAATTTCCACCAGCGTGGtt                                                             <  1:2602473/150‑1 (MQ=255)
                                                                                           tGAACAATCCGGGCTGGTCATCAGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATCACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCGGCAATTTCCACCAGCGTGGTTaa                                                           <  1:2284294/150‑1 (MQ=255)
                                                                                             aaCAATCCGGGCTGGTCATCAGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATCACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCGGCAATTTCCACCAGCGTGGTTAAac                                                         <  2:1035860/150‑1 (MQ=255)
                                                                                              aCAATCCGGGCTGGTCATCAGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATAACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCGGCAATTTCCACCAGCGTGGTTAAacc                                                        >  1:967009/1‑150 (MQ=255)
                                                                                              aCAATCCGGGCTGGTCATCAGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATAACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCGGCAATTTCCACCAGCGTGGTTAAacc                                                        <  1:1460645/150‑1 (MQ=255)
                                                                                                aaTCAGGGCTGGTCATCAGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATAACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCGGCAATTTCCACCAGCGTGGTTAAaccac                                                      >  1:3612739/1‑150 (MQ=255)
                                                                                                 aTCCGGGCTGGTCATCAGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATCACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTTGATTTGGCGATTGCGGCAATTTCCACCAGCGTGGTTAAaccacc                                                     <  2:879607/150‑1 (MQ=255)
                                                                                                   ccGGGCTGGTCATCAGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATCACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCGGCAATTTCCACCAGCGTGGTTAAACCACCGc                                                   <  1:2741206/150‑1 (MQ=255)
                                                                                                    cGGGCTGGTCATCAGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATCACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCGGCAATTTCCACCAGCGTGGTTAAACCACCGCa                                                  >  2:729334/1‑150 (MQ=255)
                                                                                                    cGGGCTGGTCATCAGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATCACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCGGCAATTTCCACCAGCGTGGTTAAACCACCGCa                                                  <  2:523248/150‑1 (MQ=255)
                                                                                                     gggCTGGTCATCAGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATCACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCGGCAATTTCCACCAGCGTGGTTAAACCACCGCAc                                                 <  2:1516270/150‑1 (MQ=255)
                                                                                                     gggCTGGTCATCAGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATCACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCGGCAATTTCCACCAGCGTGGTTAAACCACCGCAc                                                 >  2:2836500/1‑150 (MQ=255)
                                                                                                     gggCTGGTCATCAGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATAACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCGGCAATTTCCACCAGCGTGGTTAAACCACCGCAc                                                 >  1:2847014/1‑150 (MQ=255)
                                                                                                     gggCTGGTCATCAGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATAACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCGGCAATTTCCACCAGCGTGGTTAAACCACCGCAc                                                 <  1:2329389/150‑1 (MQ=255)
                                                                                                       gCTGGTCATCAGGACTTCGCTGAAGGGCGTATTGGTGAAGGTGATCCCCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCGGCAATTTCCACCAGCGTGGTTAAACCACCGCACCa                                               <  2:3610176/150‑1 (MQ=255)
                                                                                                        cTGGTCATCAGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATCACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCGGCAATTTCCACCAGCGTGGTTAAACCACCGCACCAg                                              <  1:1829756/150‑1 (MQ=255)
                                                                                                         tGGTCATCAGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATCACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCGGCAATTTCCACCAGCGTGGTTAAACCACCGCACCAGc                                             >  2:236035/1‑150 (MQ=255)
                                                                                                         tGGTCATCAGGAAATCGCTGAATGGCGAAATGGTGAAGGTGATCACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATATGGCGATAGCGGCAATTTCCACCAGCGTGGTTAAACAACCTCACCAGc                                             >  2:1476165/1‑150 (MQ=255)
                                                                                                             catcaGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATAACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCGGCAATTTCCACCAGCGTGGTTAAACCACCGCACCAGCCAAc                                         >  2:960929/1‑150 (MQ=255)
                                                                                                               tcaGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATCACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCGGCAATTTCCACCAGCGTGGTTAAACCACCGCACCAGCCAACAt                                       <  2:107659/150‑1 (MQ=255)
                                                                                                                caGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATCACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCGGCAATTTCCACCAGCGTGGTTAAACCACCGCACCAGCCAACATc                                      >  1:1027692/1‑150 (MQ=255)
                                                                                                                caGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATAACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCGGCAATTTCCACCAGCGTGGTTAAACCACCGCACCAGCCAACATc                                      >  1:448828/1‑150 (MQ=255)
                                                                                                                  ggAATTCGCTGAATGGCGTATTGGTGAAGGTGATAACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCGGCAATTTCCACCAGCGTGGTTAAACCACCGCACCAGCCAACATCCg                                    >  2:3422308/1‑150 (MQ=255)
                                                                                                                  ggAATTCGCTGAATGGCGTATTGGTGAAGGTGATAACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCGGCAATTTCCACCAGCGTGGTTAAACCACCGCACCAGCCAACATCCg                                    <  2:2298115/150‑1 (MQ=255)
                                                                                                                   gAATTCGCTGAATGGCGTATTGGTGAAGGTGATCACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCGGCAATTTCCACCAGCGTGGTTAAACCACCGCACCAGCCAACATCCgg                                   >  2:5131863/1‑150 (MQ=255)
                                                                                                                     aTTCGCTGAATGGCGTATTGGTGAAGGTGATCACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCGGCAATTTCCACCAGCGTGGTTAAACCACCGCACCAGCCAACATCCGGCt                                 >  1:575219/1‑150 (MQ=255)
                                                                                                                     aTTCGCTGAATGGCGTATTGGTGAAGGTGATCACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCGGCAATTTCCACCAGCGTGGTTAAACCACCGCACCAGCCAACATCCGGCt                                 >  1:2438260/1‑150 (MQ=255)
                                                                                                                     aTTCGCTGAATGGCGTATTGGTGAAGGTGATCACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCGGCAATTTCCACCAGCGTGGTTAAACCACCGCACCAGCCAACATCCGGCt                                 <  2:3134828/150‑1 (MQ=255)
                                                                                                                        cGCTGAATGGCGTATTGGTGAAGGTGATCACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCGGCAATTTCCACCAGCGTGGTTAAACCACCGCACCAGCCAACATCCGGCTGCa                              <  2:1414701/150‑1 (MQ=255)
                                                                                                                         gCTGAATGGCGTATTGGTGAAGGTGATCACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCGGCAATTTCCACCAGCGTGGTTAAACCACCGCACCAGCCAACATCCGGCTGCAt                             >  2:4134391/1‑150 (MQ=255)
                                                                                                                             aaTGGCGTATTGGTGAAGGTGATAACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCGGCAATTTCCACCAGCGTGGTTAAACCACCGCACCAGCCAACATCCGGCTGCATAATg                         <  1:2965466/150‑1 (MQ=255)
                                                                                                                              aTGGCGTATTGGTGAAGGTGATCACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCGGCAATTTCCACCAGCGTGGTTAAACCACCGCACCAGCCAACATCCGGCTGCATAATGt                        <  2:2653646/150‑1 (MQ=255)
                                                                                                                               tGGCGTATTGGTGAAGGTGATAACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCGGCAATTTCCACCAGCGTGGTTAAACCACCGCACCAGCCAACATCCGGCTGCATAATGTc                       >  2:3675940/1‑150 (MQ=255)
                                                                                                                                ggCGTATTGGTGAAGGTGATCACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCGGCAATTTCCACCAGCGTGGTTAAACCACCGCACCAGCCAACATCCGGCTGCATAATGTCg                      <  2:591208/150‑1 (MQ=255)
                                                                                                                                   gTATTGGTGAAGGTGATCACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCGGCAATTTCCACCAGCGTGGTTAAACCACCGCACCAGCCAACATCCGGCTGCATAATGTCGATa                   <  2:3059183/150‑1 (MQ=255)
                                                                                                                                   gTATTGGTGAAGGTGATCACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCGGCAATTTCCACCAGCGTGGTTAAACCACCGCACCAGCCAACATCCGGCTGCATAATGTCGATa                   >  1:4696374/1‑150 (MQ=255)
                                                                                                                                    tATTGGTGAAGGTGATCACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCGGCAATTTCCACCAGCGTGGTTAAACCACCGCACCAGCCAACATCCGGCTGCATAATGTCGATAc                  <  1:4068977/150‑1 (MQ=255)
                                                                                                                                       tGGTGAAGGTGATCACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCGGCAATTTCCACCAGCGTGGTTAAACCACCGCACCAGCCAACATCCGGCTGCATAATGTCGATACCgg               >  2:1921050/1‑150 (MQ=255)
                                                                                                                                       tGGTGAAGGTGATCACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCGGCAATTTCCACCAGCGTGGTTAAACCACCGCACCAGCCAACATCCGGCTGCATAATGTCGATACCgg               <  1:3983801/150‑1 (MQ=255)
                                                                                                                                        ggTGAAGGTGATCACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCGGCAATTTCCACCAGCGTGGTTAAACCACCGCACCAGCCAACATCCGGCTGCATAATGTCGATACCGGt              <  1:4973444/150‑1 (MQ=255)
                                                                                                                                         gTGAAGGTGATCACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCGGCAATTTCCACCAGCGTGGTTAAACCACCGCACCAGCCAACATCCGGCTGCATAATGTCGATACCGGtt             >  2:1980578/1‑150 (MQ=255)
                                                                                                                                          tGAAGGTGATCACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCGGCAATTTCCACCAGCGTGGTTAAACCACCGCACCAGCCAACATCCGGCTGCATAATGTCGATACCGGttt            <  1:4784631/150‑1 (MQ=255)
                                                                                                                                                 gATCACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCGGCAATTTCCACCAGCGTGGTTAAACCACCGCACCAGCCAACATCCGGCTGCATAATGTCGATACCGGTTTCTGAAAg     <  2:4188569/150‑1 (MQ=255)
                                                                                                                                                 gATAACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCGGCAATTTCCACCAGCGTGGTTAAACCACCGCACCAGCCAACATCCGGCTGCATAATGTCGATACCGGTTTCTGAAAg     <  2:3167447/150‑1 (MQ=255)
                                                                                                                                                  aTCACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCGGCAATTTCCACCAGCGTGGTTAAACCACCGCACCAGCCAACATCCGGCTGCATAATGTCGATACCGGTTTCTGAAAGc    >  2:4359445/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
AACCGGGTTTATCAAGCACTGATTTATGAATACGACCATTCACCGGAACCGGCTCATTGAGCAGAATCGGGTCAAACTGCGGACGCATCGTTGAACAATCCGGGCTGGTCATCAGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATCACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCGGCAATTTCCACCAGCGTGGTTAAACCACCGCACCAGCCAACATCCGGCTGCATAATGTCGATACCGGTTTCTGAAAGCGT  >  CP009273/2353741‑2354038

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑