Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,370,987 C→A 100% E29* (GAA→TAA)  menH ← 2‑succinyl‑6‑hydroxy‑2, 4‑cyclohexadiene‑1‑carboxylate synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,370,9870CA92.8% 874.4 / 57.6 291E29* (GAA→TAA) menH2‑succinyl‑6‑hydroxy‑2, 4‑cyclohexadiene‑1‑carboxylate synthase
Reads supporting (aligned to +/- strand):  ref base C (21/0);  new base A (142/128);  total (163/128)
Fisher's exact test for biased strand distribution p-value = 3.21e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.59e-01
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 291 total aligned reads displayed.

GGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTCGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGCTTACCTGCGCCAGAAGTTGCTGGAGCGTTTGCGCACCATCGGTGTCGTTAACCACC  >  CP009273/2370840‑2371134
                                                                                                                                                   |                                                                                                                                                   
ggATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGt                                                                                                                                                   <  2:3828884/150‑1 (MQ=255)
  aTGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTgg                                                                                                                                                 <  1:1417624/150‑1 (MQ=255)
    gttgtAACTAACCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCa                                                                                                                                               >  1:5027679/1‑150 (MQ=255)
       gtAACTAACCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTCGTGGCAATc                                                                                                                                            >  1:629242/1‑150 (MQ=255)
          actaacCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTCGTGGCAATCGcc                                                                                                                                         >  1:995250/1‑150 (MQ=255)
              ccccaGGTTTTACGCAGTAAGTCGGTGACATCATCACATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGaa                                                                                                                                     <  2:4927042/146‑1 (MQ=255)
                cAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGaaaa                                                                                                                                   <  1:912795/150‑1 (MQ=255)
                  aGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAAc                                                                                                                                 <  2:2200757/150‑1 (MQ=255)
                   ggTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAAcc                                                                                                                                >  1:3123784/1‑150 (MQ=255)
                   ggTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAAcc                                                                                                                                <  1:1958912/150‑1 (MQ=255)
                    gTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCg                                                                                                                               >  2:1609014/1‑150 (MQ=255)
                      tttACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTg                                                                                                                             <  1:316696/150‑1 (MQ=255)
                      tttACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTg                                                                                                                             <  2:2590915/150‑1 (MQ=255)
                      tttACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTg                                                                                                                             >  2:2014319/1‑150 (MQ=255)
                         aCGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCaa                                                                                                                          >  1:4655377/1‑150 (MQ=255)
                            cAGTAAGTTGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCaaaaa                                                                                                                       >  2:2345847/1‑150 (MQ=255)
                             aGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAac                                                                                                                      >  1:2172704/1‑150 (MQ=255)
                              gTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAaca                                                                                                                     <  2:350970/150‑1 (MQ=255)
                               tAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAacac                                                                                                                    >  2:4378677/1‑150 (MQ=255)
                                aaGTCGGTGACATCCTCAAATCCCTCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACAcc                                                                                                                   <  2:4777595/150‑1 (MQ=255)
                                aaGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACAcc                                                                                                                   <  1:2726402/150‑1 (MQ=255)
                                aaGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACAcc                                                                                                                   >  1:1114929/1‑150 (MQ=255)
                                aaGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACAcc                                                                                                                   >  2:2369305/1‑150 (MQ=255)
                                 aGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACAcca                                                                                                                  >  1:2521038/1‑150 (MQ=255)
                                 aGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACAcca                                                                                                                  >  2:3437346/1‑150 (MQ=255)
                                  gTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACAccag                                                                                                                 <  2:2888450/150‑1 (MQ=255)
                                   tCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACAccagc                                                                                                                <  1:3144157/150‑1 (MQ=255)
                                     ggTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACAccagcca                                                                                                              <  2:2408911/150‑1 (MQ=255)
                                     ggTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACAccagcca                                                                                                              <  2:3038388/150‑1 (MQ=255)
                                         aCATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGt                                                                                                          <  2:4472158/150‑1 (MQ=255)
                                          catcatCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTa                                                                                                         >  1:753615/1‑150 (MQ=255)
                                          catcatCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTa                                                                                                         <  2:4757452/150‑1 (MQ=255)
                                          catcatCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTGCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCTAGGGTa                                                                                                         >  1:4233489/1‑150 (MQ=255)
                                           atcatcAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTCGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTaa                                                                                                        >  1:287386/1‑150 (MQ=255)
                                           atcatcAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTaa                                                                                                        <  2:123185/150‑1 (MQ=255)
                                            tcatcaAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTaaa                                                                                                       >  1:1014263/1‑150 (MQ=255)
                                              atcaAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAAcc                                                                                                     >  1:2421461/1‑150 (MQ=255)
                                                caAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTg                                                                                                   <  2:148383/150‑1 (MQ=255)
                                                   aTCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGtt                                                                                                >  1:4933975/1‑150 (MQ=255)
                                                      cATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTc                                                                                             >  2:4331127/1‑150 (MQ=255)
                                                      cATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTc                                                                                             <  2:4819022/150‑1 (MQ=255)
                                                       aTCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTcc                                                                                            <  2:896909/150‑1 (MQ=255)
                                                       aTCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTcc                                                                                            <  1:2042414/150‑1 (MQ=255)
                                                       aTCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTcc                                                                                            >  1:428620/1‑150 (MQ=255)
                                                        tCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCTATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCg                                                                                           >  2:130189/1‑150 (MQ=255)
                                                         cGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCgt                                                                                          <  1:380741/150‑1 (MQ=255)
                                                            cGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGtt                                                                                       >  1:534868/1‑150 (MQ=255)
                                                               tAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTg                                                                                    <  2:182167/150‑1 (MQ=255)
                                                                   cgccgcCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTg                                                                                >  1:2443476/1‑150 (MQ=255)
                                                                      cgccgAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTgcgc                                                                             >  1:3395075/1‑150 (MQ=255)
                                                                       gccgAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGcgcg                                                                            <  2:711092/150‑1 (MQ=255)
                                                                        ccgAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGt                                                                           <  1:1011318/150‑1 (MQ=255)
                                                                         cgcaccaccGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTg                                                                          <  2:830825/147‑1 (MQ=255)
                                                                         cgAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTg                                                                          >  1:625115/1‑150 (MQ=255)
                                                                         cgAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTg                                                                          >  1:1854366/1‑150 (MQ=255)
                                                                          gAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGc                                                                         >  2:1567603/1‑150 (MQ=255)
                                                                          gAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGc                                                                         <  2:4181394/150‑1 (MQ=255)
                                                                           aaCCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCa                                                                        >  1:2563754/1‑150 (MQ=255)
                                                                            ccccccGGGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCGGGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAACCACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAg                                                                       <  2:2672153/146‑1 (MQ=255)
                                                                              caccGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGa                                                                     >  2:1062827/1‑150 (MQ=255)
                                                                                ccGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATc                                                                   <  1:1496841/150‑1 (MQ=255)
                                                                                 cGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCa                                                                  <  1:400566/150‑1 (MQ=255)
                                                                                   tGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATa                                                                >  2:4594031/1‑150 (MQ=255)
                                                                                     aCCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATaaa                                                              >  1:4564935/1‑150 (MQ=255)
                                                                                       cTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTCGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATg                                                            >  2:2808976/1‑150 (MQ=255)
                                                                                        tGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATgg                                                           >  1:4164461/1‑150 (MQ=255)
                                                                                         gggAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAGCCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGc                                                          <  2:2862550/150‑1 (MQ=255)
                                                                                          ggAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGCt                                                         >  2:1414819/1‑150 (MQ=255)
                                                                                               tCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTCGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGCTTACCt                                                    >  2:1327489/1‑150 (MQ=255)
                                                                                               tCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGCTTACCt                                                    >  2:532622/1‑150 (MQ=255)
                                                                                               tCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGCTTACCt                                                    >  2:4983589/1‑150 (MQ=255)
                                                                                                cAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGCTTACCTg                                                   <  1:367032/150‑1 (MQ=255)
                                                                                                cAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGCTTACCTg                                                   <  1:1903015/150‑1 (MQ=255)
                                                                                                 aaCATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGCTTACCTgc                                                  >  2:3795807/1‑150 (MQ=255)
                                                                                                  aCATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGCTTACCTgcg                                                 <  1:2296620/150‑1 (MQ=255)
                                                                                                  aCATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGCTTACCTgcg                                                 <  1:3461062/150‑1 (MQ=255)
                                                                                                    aTACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGCTTACCTGCGcc                                               >  1:295060/1‑150 (MQ=255)
                                                                                                     tACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTCGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGCTTACCTGCGCCa                                              >  2:782688/1‑150 (MQ=255)
                                                                                                      aCAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGCTTACCTGCGCCAg                                             >  2:122275/1‑150 (MQ=255)
                                                                                                       cAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGCTTACCTGCGCCAGa                                            <  1:769506/150‑1 (MQ=255)
                                                                                                         aCCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGCTTACCTGCGCCCGAAg                                          >  2:4052031/1‑150 (MQ=255)
                                                                                                                gtagtCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGCTTACCTGCGCCAGAAGTTGCTgg                                   <  2:1646332/150‑1 (MQ=255)
                                                                                                                     cGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGCTTACCTGCGCCAGAAGTTGCTGGAGCGt                              <  1:3918214/150‑1 (MQ=255)
                                                                                                                      ggCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGCTTACCTGCGCCAGAAGTTGCTGGAGCGtt                             <  2:4604598/150‑1 (MQ=255)
                                                                                                                       gCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGCTTACCTGCGCCAGAAGTTGCTGGAGCGttt                            <  2:3658088/150‑1 (MQ=255)
                                                                                                                         aaaCGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGCTTACCTGCGCCAGAAGTTGCTGGAGCGTTTgc                          >  2:2066394/1‑150 (MQ=255)
                                                                                                                           aCGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGCTTACCTGCGCCAGAAGTTGCTGGAGCGTTTgcgc                        >  1:1230454/1‑150 (MQ=255)
                                                                                                                              ccTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGCTTACCTGCGCCAGAAGTTGCTGGAGCGTTTGCGCAcc                     >  1:3208663/1‑150 (MQ=255)
                                                                                                                               cTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGCTTACCTGCGCCAGAAGTTGCTGGAGCGTTTGCGCACCa                    >  1:632093/1‑150 (MQ=255)
                                                                                                                                tCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGCTTACCTGCGCCAGAAGTTGCTGGAGCGTTTGCGCACCAt                   <  1:4382495/150‑1 (MQ=255)
                                                                                                                                tCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGCTTACCTGCGCCAGAAGTTGCTGGAGCGTTTGCGCACCAt                   >  2:3340476/1‑150 (MQ=255)
                                                                                                                                    ccACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGCTTACCTGCGCCAGAAGTTGCTGGAGCGTTTGCGCACCATCGgt               <  1:4414503/150‑1 (MQ=255)
                                                                                                                                     cACTTCTTGCCATTCGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGCTTACCTGCGCCAGAAGTTGCTGGAGCGTTTGCGCACCATCGgtg              >  2:2392509/1‑150 (MQ=255)
                                                                                                                                      aCTTCTTGCCATTCGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGCTTACCTGCGCCAGAAGTTGCTGGAGCGTTTGCGCACCATCGgtgt             >  1:1642601/1‑150 (MQ=255)
                                                                                                                                        ttcttGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGCTTACCTGCGCCAGAAGTTGCTGGAGCGTTTGCGCACCATCGGTGTCg           >  1:2774668/1‑150 (MQ=255)
                                                                                                                                          cttGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGCTTACCTGCGCCAGAAGTTGCTGGAGCGTTTGCGCACCATCGGTGTCGtt         >  2:3935020/1‑150 (MQ=255)
                                                                                                                                           ttGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGAGTTTTGCCTGCGCGTGCAGGATCATAAATGGCTTACCTGCGCCAGAAGTTGCTGGAGCGTTTGCGCACCATCGGTGTCGTTa        <  1:1129521/150‑1 (MQ=255)
                                                                                                                                            tGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGCTTACCTGCGCCAGAAGTTGCTGGAGCGTTTGCGCACCATCGGTGTCGTTaa       >  2:2299404/1‑150 (MQ=255)
                                                                                                                                                aTTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGCTTACCTGCGCCAGAAGTTGCTGGAGCGTTTGCGCACCATCGGTGTCGTTAaccac   >  1:3084668/1‑150 (MQ=255)
                                                                                                                                                 ttCGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGCTTACCTGCGCCAGAAGTTGCTGGAGCGTTTGCGCACCATCGGTGTCGTTAaccacc  >  2:1362672/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
GGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTCGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGCTTACCTGCGCCAGAAGTTGCTGGAGCGTTTGCGCACCATCGGTGTCGTTAACCACC  >  CP009273/2370840‑2371134

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑