BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	2,379,450	C→T	9.6%	P38S (CCT→TCT)	yfbL →	putative peptidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	2,379,450	0	C	T	9.6%	984.4 / 72.4	291	P38S (CCT→TCT)	yfbL	putative peptidase
Reads supporting (aligned to +/- strand): ref base C (143/119); new base T (16/12); total (160/131)
Fisher's exact test for biased strand distribution p-value = 8.44e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.88e-01

Only 100 of 294 total aligned reads displayed.

ATAATGATTCAACAACGCAACCCATTAATAATTTGCATATGAAAAAAATAATCTTTGCTTTTATTATATTATTTGTGTTTTTACTCCCTATGATTATTTTTTACCAACCCTGGGTCAATGCATTACCGTCAACGCCCCGACACGCAAGTCCTGAGCAATTAGAAAAAACAGTTCGTTATCTTACACAAACTGTGCATCCACGTAGTGCCGACAATATTGATAATCTGAATAGGTCCGCAGAATATATAAAAGAGGTCTTTGTCAGTAGCGGTGCCAGAGTTACCTCGCAGGACGTCCCC  >  CP009273/2379301‑2379599

  aatGATTCAACAACGCAACCCATTAATAATTTGCATATGAAAAAAATAATCTTTGCTTTTATTATATTATTTGTGTTTTTACTCCCTATGATTATTTTTTACCAACCCTGGGTCAATGCATTACCGTCAACGCCCCGACACGCAAGTCCt                                                                                                                                                     >  1:4630246/1‑150 (MQ=255)

     gATTCAACAACGCAACCCATTAATAATTTGCATATGAAAAAAATAATCTTTGCTTTTATTATATTATTTGTGTTTTTACTCCCTATGATTATTTTTTACCAACCCTGGGTCAATGCATTACCGTCAACGCCCCGACACGCAAGTCCTGAg                                                                                                                                                  <  2:1037420/150‑1 (MQ=255)

       ttCAACAACGCAACCCATTAATAATTTGCATATGAAAAAAATAATCTTTGCTTTTATTATATTATTTGTGTTTTTACTCCCTATGATTATTTTTTACCAACCCTGGGTCAATGCATTACCGTCAACGCCCCGACACGCAAGTCCTGAGCa                                                                                                                                                <  2:4567441/150‑1 (MQ=255)

       ttCAACAACGCAACCCATTAATAATTTGCATATGAAAAAAATAATCTTTGCTTTTATTATATTATTTGTGTTTTTACTCCCTATGATTATTTTTTACCAACCCTGGGTCAATGCATTACCGTCAACGCCCCGACACGCAAGTCCTGAGCa                                                                                                                                                <  2:2840060/150‑1 (MQ=255)

       ttCAACAACGCAACCCATTAATAATTTGCATATGAAAAAAATAATCTTTGATTTTATTATATTATTTGTGTTTTTACTCCCTATGATTATTTTTTACCAACCCTGGGTCAATGCATTACCGTCAACGCCCCGACACGCAAGTCCTGAGCa                                                                                                                                                <  2:2012580/150‑1 (MQ=255)

        tCAACAACGCAACCCATTAATAATTTGCATATGAAAAAAATAATCTTTGCTTTTATTATATTATTTGTGTTTTTACTCCCTATGATTATTTTTTACCAACCCTGGGTCAATGCATTACCGTCAACGCCCCGACACGCAAGTCCTGAGCaa                                                                                                                                               <  2:5046544/150‑1 (MQ=255)

         caacaaCGCAACCCATTAATAATTTGCATATGAAAAAAATAATCTTTGCTTTTATTATATTATTTGTGTTTTTACTCCCTATGATTATTTTTTACCAACCCTGGGTCAATGCATTACCGTCAACGCCCCGACACGCAAGTCCTGAGCAAt                                                                                                                                              >  1:2655723/1‑150 (MQ=255)

           acaacGCAACCCATTAATAATTTGCATATGAAAAAAATAATCTTTGCTTTTATTATATTATTTGTGTTTTTACTCCCTATGATTATTTTTTACCAACCCTGGGTCAATGCATTACCGTCAACGCCCCGACACGCAAGTCCTGAGCAATTa                                                                                                                                            <  1:2520668/150‑1 (MQ=255)

            caacGCAACCAATTAATAATTTGCATATGAAAAAAATAATCTTTGCTTTTATTATATTATTTGTGTTTTTACTCCCTATGATTATTTTTTACCAACCCTGGGTCAATGCATTACCGTCAACGCCCCGACACGCAAGTCCTGAGCAATTAg                                                                                                                                           <  1:4104319/150‑1 (MQ=255)

             aacGCAACCCATTAATAATTTGCATATGAAAAAAATAATCTTTGCTTTTATTATATTATTTGTGTTTTTACTCCCTATGATTATTTTTTACCAACCCTGGGTCAATGCATTACCGTCAACGCCCCGACACGCAAGTTCTGAGCAATTAGa                                                                                                                                          <  2:1182788/150‑1 (MQ=255)

                  aaCCCATTAATAATTTGCATATGAAAAAAATAATCTTTGCTTTTATTATATTATTTGTGTTTTTACTCCCTATGATTATTTTTTACCAACCCTGGGTCAATGCATTACCGTCAACGCCCCGACACGCAAGTCCTGAGCAATTAGaaaaaa                                                                                                                                     >  1:2127819/1‑150 (MQ=255)

                      cATTAATAATTTGCATATGAAAAAAATAATCTTTGCTTTTATTATATTATTTGTGTTTTTACTCCCTATGATTATTTTTTACCAACCCTGGGTCAATGCATTACCGTCAACGCCCCGACACGCAAGTCCTGAGCAATTAGAAAAAACAGt                                                                                                                                 >  2:482423/1‑150 (MQ=255)

                         taataaTTTGCATATGAAAAAAATAATCTTTGCTTTTATTATATTATTTGTGTTTTTACTCCCTATGATTATTTTTTACCAACCCTGGGTCAATGCATTACCGTCAACGCCCCGACACGCAAGTCCTGAGCAATTAGAAAAAACAGTTCg                                                                                                                              >  1:13994/1‑150 (MQ=255)

                          aataatTTGCATATGAAAAAAATAATCTTTGCTTTTATTATATTATTTGTGTTTTTACTCCCTATGATTATTTTTTACCAACCCTGGGTCAATGCATTACCGTCAACGCCCCGACACGCAAGTCCTGAGCAATTAGAAAAAACAGTTCGt                                                                                                                             >  2:2751503/1‑150 (MQ=255)

                             aatTTGCATATGAAAAAAATAATCTTTGCTTTTATTATATTATTTGTGTTTTTACTCCCTATGATTATTTTTTACCAACCCTGGGTCAATGCATTACCGTCAACGCCCCGACACGCAAGTTCTGAGCAATTAGAAAAAACAGTTCGTTAt                                                                                                                          >  1:4423695/1‑150 (MQ=255)

                             aatTTGCATATGAAAAAAATAATCTTTGCTTTTATTATATTATTTGTGTTTTTACTCCCTATGATTATTTTTTACCAACCCTGGGTCAATGCATTACCGTCAACGCCCCGACACGCAAGTCCTGAGCAATTAGAAAAAACAGTTCGTTAt                                                                                                                          <  1:3236468/150‑1 (MQ=255)

                                ttGCATATGAAAAAAATAATCTTTGCTTTTATTATATTATTTGTGTTTTTACTCCCTATGATTATTTTTTACCAACCCTGGGTCAATGCATTACCGTCAACGCCCCGACACGCAAGTCCTGAGCAATTAGAAAAAACAGTTCGTTATCtt                                                                                                                       >  1:1426832/1‑150 (MQ=255)

                                  gCATATGAAAAAAATAATCTTTGCTTTTATTATATTATTTGTGTTTTTACTCCCTATGATTATTTTTTACCAACCCTGGGTCAATGCATTACCGTCAACGCCCCGACACGCAAGTCCTGAGCAATTAGAAAAAACAGTTCGTTATCTTac                                                                                                                     <  2:2289615/150‑1 (MQ=255)

                                  gCATATGAAAAAAATAATCTTTGCTTTTATTATATTATTTGTGTTTTTACTCCCTATGATTATTTTTTACCAACCCTGGGTCAATGCATTACCGTCAACGCCCCGACACGCAAGTCCTGAGCAATTAGAAAAAACAGTTCGTTATCTTac                                                                                                                     <  2:2355940/150‑1 (MQ=255)

                                   cATATGAAAAAAATAATCTTTGCTTTTATTATATTATTTGTGTTTTTACTCCCTATGATTATTTTTTACCAACCCTGGGTCAATGCATTACCGTCAACGCCCCGACACGCAAGTCCTGAGCAATTAGAAAAAACAGTTCGTTATCTTaca                                                                                                                    >  1:4270231/1‑150 (MQ=255)

                                     tatGAAAAAAATAATCTTTGCTTTTATTATATTATTTGTGTTTTTACTCCCTATGATTATTTTTTACCAACCCTGGGTCAATGCATTACCGTCAACGCCCCGACACGCAAGTCCTGAGCAATTAGAAAAAACAGTTCGTTATCTTAcaca                                                                                                                  <  1:1871899/150‑1 (MQ=255)

                                        gAAAAAAATAATCTTTGCTTTTATTATATTATTTGTGTTTTTACTCCCTATGATTATTTTTTACCAACCCTGGGTCAATGCATTACCGTCAACGCCCCGACACGCAAGTCCTGAGCAATTAGAAAAAACAGTTCGTTATCTTACACAAAc                                                                                                               >  1:1336006/1‑150 (MQ=255)

                                         aaaaaaaTAATCTTTGCTTTTATTATATTATTTGTGTTTTTACTCCCTATGATTATTTTTTACCAACCCTGGGTCAATGCATTACCGTCAACGCCCCGACACGCAAGTCCTGAGCAATTAGAAAAAACAGTTCGTTATCTTACACAAACt                                                                                                              >  1:4569321/1‑150 (MQ=255)

                                          aaaaaaTAATCTTTGCTTTTATTATATTATTTGTGTTTTTACTCCCTATGATTATTTTTTACCAACCCTGGGTCAATGCATTACCGTCAACGCCCCGACACGCAAGTCCTGAGCAATTAGAAAAAACAGTTCGTTATCTTACACAAACtg                                                                                                             >  1:2828446/1‑150 (MQ=255)

                                            aaaaTAATCTTTGCTTTTATTATATTATTTGTGTTTTTACTCCCTATGATTATTTTTTACCAACCCTGGGTCAATGCATTACCGTCAACGCCCCGACACGCAAGTCCTGAGCAATTAGAAAAAACAGTTCGTTATCTTACACAAACtgtg                                                                                                           <  1:2208719/150‑1 (MQ=255)

                                            aaaaTAATCTTTGCTTTTATTATATTATTTGTGTTTTTACTCCCTATGATTATTTTTTACCAACCCTGGGTCAATGCATTACCGTCAACGCCCCGACACGCAAGTCCTGAGCAATTAGAAAAAACAGTTCGTTATCTTACACAAACtgtg                                                                                                           >  2:1631047/1‑150 (MQ=255)

                                            aaaaTAATCTTTGCTTTTATTATATTATTTGTGTTTTTACTCCCTATGATTATTTTTTACCAACCCTGGGTCAATGCATTACCGTCAACGCCCCGACACGCAAGTCCTGAGCAATTAGAAAAAACAGTTCGTTATCTTACACAAACtgtg                                                                                                           >  2:1003847/1‑150 (MQ=255)

                                             aaaTAATCTTTGCTTTTATTATATTATTTGTGTTTTTACTCCCTATGATTATTTTTTACCAACCCTGGGTCAATGCATTACCGTCAACGCCCCGACACGCAAGTCCTGAGCAATTAGAAAAAACAGTTCGTTATCTTACACAAACTGTGc                                                                                                          <  1:4650996/150‑1 (MQ=255)

                                              aataatCTTTGCTTTTATTATATTATTTGTGTTTTTACTCCCTATGATTATTTTTTACCAACCCTGGGTCAATGCATTACCGTCAACGCCCCGACACGCAAGTCCTGAGCAATTAGAAAAAACAGTTCGTTATCTTACACAAACTGTGCa                                                                                                         >  2:3761021/1‑150 (MQ=255)

                                               ataatCTTTGCTTTTATTATATTATTTGTTTTTTTACTACCTATGATTATTTTTTACCAACCCTGGGTCAATGCATTACCGTCAACGCCCCGACACGCAAGTCCTGAGCAATTAGAAAAAACAGTTCGTTATCTTACACAAACTGTGCAt                                                                                                        <  2:49794/150‑1 (MQ=255)

                                                 aatCTTTGCTTTTATTATATTATTTGTGTTTTTACTCCCTATGATTATTTTTTACCAACCCTGGGTCAATGCATTACCGTCAACGCCCCGACACGCAAGTCCTGAGCAATTAGAAAAAACAGTTCGTTATCTTACACAAACTGTGCATcc                                                                                                      <  1:1271908/150‑1 (MQ=255)

                                                  atCTTTGCTTTTATTATATTATTTGTGTTTTTACTCCCTATGATTATTTTTTACCAACCCTGGGTCAATGCATTACCGTCAACGCCCCGACACGCAAGTCCTGAGCAATTAGAAAAAACAGTTCGTTATCTTACACAAACTGTGCATCCa                                                                                                     >  2:1156369/1‑150 (MQ=255)

                                                   tCTTTGCTTTTATTATATTATTTGTGTTTTTACTCCCTATGATTATATTTTACCAACCCTGGGTCAATGCATTACCGTCAACGCCCCGACACGCAAGTCCTGAGCAATTAGAAAAAACAGTTCGTTATCTTACACAAACTGTGCATCCAc                                                                                                    <  2:5147899/150‑1 (MQ=255)

                                                     tttGCTTTTATTATATTATTTGTGTTTTTACTCCCTATGATTATTTTTTACCAACCCTGGGTAAATGCATTACCGTAAACTCCACGACACGCAAGTACTGAGCAAATAGAAAAAACAGTTCTTTATATTACAAAAACTGTGCATCCACGt                                                                                                  >  2:4641912/1‑150 (MQ=255)

                                                      ttGCTTTTATTATATTATTTGTGTTTTTACTCCCTATGATTATTTTTTACCAACCCTGGGTCAATGCATTACCGTCAACGCCCCGACACGCAAGTCCTGAGCAATTAGAAAAAACAGTTCGTTATCTTACACAAACTGTGCATCCACGTa                                                                                                 >  1:4897541/1‑150 (MQ=255)

                                                         cTTTTATTATATTATTTGTGTTTTTACTCCCTATGATTATTTTTTACCAACCCTGGGTCAATGCATTACCGTCAACGCCCCGACACGCAAGTCCTGAGCAATTAGAAAAAACAGTTCGTTATCTTACACAAACTGTGCATCCACGTAGTg                                                                                              >  1:5036103/1‑150 (MQ=255)

                                                             tattatattaTTTGTGTTTTTACTCCCTATGATTATTTTTTACCAACCCTGGGTCAATGCATTACCGTCAACGCCCCGACACGCAAGTCCTGAGCAATTAGAAAAAACAGTTCGTTATCTTACACAAACTGTGCATCCACGTAGTGCCGa                                                                                          <  1:843657/150‑1 (MQ=255)

                                                             tattatattaTTTGTGTTTTTACTCCCTATGATTATTTTTTACCAACCCTGGGTCAATGCATTACCGTCAACGCCCCGACACGCAAGTCCTGAGCAATTAGAAAAAACAGTTCGTTATCTTACACAAACTGTGCATCCACGTAGTGCCGa                                                                                          >  1:1596694/1‑150 (MQ=255)

                                                               ttatattatTTGTGTTTTTACTCCCTATGATTATTTTTTACCAACCCTGGGTCAATGCATTACCGTCAACGCCCCGACACGCAAGTCCTGAGCAATTAGAAAAAACAGTTCGTTATCTTACACAAACTGTGCATCCACGTAGTGCCGACa                                                                                        >  1:3518605/1‑150 (MQ=255)

                                                                 atattatTTGTGTTTTTACTCCCTATGATTATTTTTTACCAACCCTGGGTCAATGCATTACCGTCAACGCCCCGACACGCAAGTCCTGAGCAATTAGAAAAAACAGTTCGTTATCTTACACAAACTGTGCATCCACGTAGTGCCGACAat                                                                                      >  1:621536/1‑150 (MQ=255)

                                                                  tattatTTGTGTTTTTACTCCCTATGATTATTTTTTACCAACCCTGGGTCAATGCATTACCGTCAACGCCCCGACACGCAAGTCCTGAGCAATTAGAAAAAACAGTTCGTTATCTTACACAAACTGTGCATCCACGTAGTGCCGACAata                                                                                     <  1:4683336/150‑1 (MQ=255)

                                                                       tttGTGTTTTTACTCCCTATGATTATTTTTTACCAACCCTGGGTCAATGCATTACCGTCAACGCCCCGACACGCAAGTCCTGAGCAATTAGAAAAAACAGTTCGTTATCTTACACAAACTGTGCATCCACGTAGTGCCGACAATATTGAt                                                                                <  1:4336220/150‑1 (MQ=255)

                                                                        ttGTGTTTTTACTCCCTATGATTATTTTTTACCAACCCTGGGTCAATGCATTACCGTCAACGCCCCGACACGCAAGTCCTGAGCAATTAGAAAAAACAGTTCGTTATCTTACACAAACTGTGCATCCACGTAGTGCCGACAATATTGATa                                                                               >  2:436258/1‑150 (MQ=255)

                                                                         tgtgTTTTTACTCCCTATGATTATTTTTTACCAACCCTGGGTCAATGCATTACCGTCAACGCCCCGACACGCAAGTCCTGAGCAATTAGAAAAAACAGTTCGTTATCTTACACAAACTGTGCATCCACGTAGTGCCGACAATATTGATaa                                                                              <  2:4531086/150‑1 (MQ=255)

                                                                         tgtgTTTTTACTCCCTATGATTATTTTTTACCAACCCTGGGTCAATGCATTACCGTCAACGCCCCGACACGCAAGTCCTGAGCAATTAGAAAAAACAGTTCGTTATCTTACACAAACTGTGCATCCACGTAGTGCCGACAATATTGATaa                                                                              >  2:3949199/1‑150 (MQ=255)

                                                                         tgtgTTTTTACTCCCTATGATTATTTTTTACCAACCCTGGGTCAATGCATTACCGTCAACGCCCCGACACGCAAGTCCTGAGCAATTAGAAAAAACAGTTCGTTATCTTACACAAACTGTGCATCCACGTAGTGCCGACAATATTGATaa                                                                              >  1:2578147/1‑150 (MQ=255)

                                                                         tgtgTTTTTACTCCCTATGATTATTTTTTACCAACCCTGGGTCAATGCATTACCGTCAACGCCCCGACACGCAAGTCCTGAGCAATTAGAAAAAACAGTTCGTTATCTTACACAAACTGTGCATCCACGTAGTGCCGACAATATTGATaa                                                                              >  2:1522352/1‑150 (MQ=255)

                                                                         tgtgTTTTAACTCCCTATGAATATTTATTACCAACCCTGGGTCAATGCATTACCGTCAACGCCCCGACACGCAAGTCCTGAGCAATTAGAAAAAACAGTTCGTTATCTTACACAAACAGTGCATCCACGTAGTGCCGACAATATTGATac                                                                              >  2:2621455/1‑149 (MQ=255)

                                                                           tgtTTTTACTCCCTATGATTATTTTTTACCAACCCTGGGTCAATGCATTACCGTCAACGCCCCGACACGCAAGTCCTGAGCAATTAGAAAAAACAGTTCGTTATCTTACACAAACTGTGCATCCACGTAGTGCCGACAATATTGATAATc                                                                            <  2:2449122/150‑1 (MQ=255)

                                                                           tgtTTTTACTCCCTATGATTATTTTTTACCAACCCTGGGTCAATGCATTACCGTCAACGCCCCGACACGCAAGTCCTGAGCAATTAGAAAAAACAGTTCGTTATCTTACACAAACTGTGCATCCACGTAGTGCCGACAATATTGATAATc                                                                            >  2:1120910/1‑150 (MQ=255)

                                                                            gtTTTTACTCCCTATGATTATTTTTTACCAACCCTGGGTCAATGCATTACCGTCAACGCCCCGACACGCAAGTCCTGAGCAATTAGAAAAAACAGTTCGTTATCTTACACAAACTGTGCATCCACGTAGTGCCGACAATATTGATAATCt                                                                           <  2:1729897/150‑1 (MQ=255)

                                                                             tttttACTCCCTATGATTATTTTTTACCAACCCTGGGTCAATGCATTACCGTCAACGCCCCGACACGCAAGTCCTGAGCAATTAGAAAAAACAGTTCGTTATCTTACACAAACTGTGCATCCACGTAGTGCCGACAATATTGATAATCTg                                                                          >  1:4839435/1‑150 (MQ=255)

                                                                              ttttACTCCCTATGATTATTTTTTACCAACCCTGGGTCAATGCATTACCGTCAACGCCCCGACACGCAAGTCCTGAGCAATTAGAAAAAACAGTTCGTTATCTTACACAAACTGTGCATCCACGTAGTGCCGACAATATTGATAATCTGa                                                                         <  1:3438341/150‑1 (MQ=255)

                                                                               tttACTCCCTATGATTATTTTTTACCAACCCTGGGTCAATGCATTACCGTCAACGCCCCGACACGCAAGTTCTGAGCAATTAGAAAAAACAGTTCGTTATCTTACACAAACTGTGCATCCACGTAGTGCCGACAATATTGATAATCTGaa                                                                        <  2:3135387/150‑1 (MQ=255)

                                                                                  aCTCCCTATGATTATTTTTTACCAACCCTGGGTCAATGCATTACCGTCAACGCCCCGACACGCAAGTCCTGAGCAATTAGAAAAAACAGTTCGTTATCTTACACAAACTGTGCATCCACGTAGTGCCGACAATATTGATAATCTGAATAg                                                                     <  2:4154704/150‑1 (MQ=255)

                                                                                  aCTCCCTATGATTATTTTTTACCAACCCTGGGTCAATGCATTACCGTCAACGCCCCGACACGCAAGTCCTGAGCAATTAGAAAAAACAGTTCGTTATCTTACACAAACTGTGCATCCACGTAGTGCCGACAATATTGATAATCTGAATAg                                                                     >  1:1978642/1‑150 (MQ=255)

                                                                                    tCCCTATGATTATTTTTTACCAACCCTGGGTCAATGCATTACCGTCAACGCCCCGACACGCAAGTCCTGAGCAATTAGAAAAAACAGTTCGTTATCTTACACAAACTGTGCATCCACGTAGTGCCGACAATATTGATAATCTGAATAGGt                                                                   >  2:1957251/1‑150 (MQ=255)

                                                                                    tCCCTATGATTATTTTTTACCAACCCTGGGTCAATGCATTACCGTCAACGCCCCGACACGCAAGTCCTGAGCAATTAGAAAAAACAGTTCGTTATCTTACACAAACTGTGCATCCACGTAGTGCCGACAATATTGATAATCTGAATAGGt                                                                   >  1:23765/1‑150 (MQ=255)

                                                                                     cccTATGATTATTTTTTACCAACCCTGGGTCAATGCATTACCGTCAACGCCCCGACACGCAAGTCCTGAGCAATTAGAAAAAACAGTTCGTTATCTTACACAAACTGTGCATCCACGTAGTGCCGACAATATTGATAATCTGAATAGGTc                                                                  >  1:938582/1‑150 (MQ=255)

                                                                                      ccTATGATTATTTTTTACCAACCCTGGGTCAATGCATTACCGTCAACGCCCCGACACGCAAGTTCTGAGCAATTAGAAAAAACAGTTCGTTATCTTACACAAACTGTGCATCCACGTAGTGCCGACAATATTGATAATCTGAATAGGTcc                                                                 >  1:3648596/1‑150 (MQ=255)

                                                                                      ccTATGATTATTTTTTACCAACCCTGGGTCAATGCATTACCGTCAACGCCCCGACACGCAAGTCCTGAGCAATTAGAAAAAACAGTTCGTTATCTTACACAAACTGTGCATCCACGTAGTGCCGACAATATTGATAATCTGAATAGGTcc                                                                 >  1:2011598/1‑150 (MQ=255)

                                                                                      ccTATGATTATTTTTTACCAACCCTGGGTCAATGCATTACCGTCAACGCCCCGACACGCAAGTCCTGAGCAATTAGAAAAAACAGTTCGTTATCTTACACAAACTGTGCATCCACGTAGTGCCGACAATATTGATAATCTGAATAGGTcc                                                                 <  2:3819171/150‑1 (MQ=255)

                                                                                      ccTATGATTATTTTTTACCAACCCTGGGTCAATGCATTACCGTCAACGCCCCGACACGCAAGTCCTGAGCAATTAGAAAAAACAGTTCGTTATCTTACACAAACTGTGCATCCACGTAGTGCCGACAATATTGATAATCTGAATAGGTcc                                                                 >  1:2300324/1‑150 (MQ=255)

                                                                                       cTATGATTATTTTTTACCAACCCTGGGTCAATGCATTACCGTCAACGCCCCGACACGCAAGTCCTGAGCAATTAGAAAAAACAGTTCGTTATCTTACACAAACTGTGCATCCACGTAGTGCCGACAATATTGATAATCTGAATAGGTCCg                                                                <  1:3046046/150‑1 (MQ=255)

                                                                                       cTATGATTATTTTTTACCAACCCTGGGTCAATGCATTACCGTCAACGCCCCGACACGCAAGTCCTGAGCAATTAGAAAAAACAGTTCGTTATCTTACACAAACTGTGCATCCACGTAGTGCCGACAATATTGATAATCTGAATAGGTCCg                                                                <  1:421326/150‑1 (MQ=255)

                                                                                         aTGATTATTTTTTACCAACCCTGGGTCAATGCATTACCGTCAACGCCCCGACACGCAAGTCCTGAGCAATTAGAAAAAACAGTTCGTTATCTTACACAAACTGTGCATCCACGTAGTGCCGACAATATTGATAATCTGAATAGGTCCGCa                                                              >  1:293840/1‑150 (MQ=255)

                                                                                          tGATTATTTTTTACCAACCCTGGGTCAATGCATTACCGTCAACGCCCCGACACGCAAGTCCTGAGCAATTAGAAAAAACAGTTCGTTATCTTACACAAACTGTGCATCCACGTAGTGCCGACAATATTGATAATCTGAATAGGTCCGCAg                                                             >  2:3085369/1‑150 (MQ=255)

                                                                                                ttttttACCAACCCTGGGTCAATGCATTACCGTCAACGCCCCGACACGCAAGTCCTGAGCAATTAGAAAAAACAGTTCGTTATCTTACACAAACTGTGCATCCACGTAGTGCCGACAATATTGATAATCTGAATAGGTCCGCAGAatata                                                       <  2:798530/150‑1 (MQ=255)

                                                                                                ttttttACCAACCCTGGGTCAATGCATTACCGTCAACGCCCCGACACGCAAGTCCTGAGCAATTAGAAAAAACAGTTCGTTATCTTACACAAACTGTGCATCCACGTAGTGCCGACAATATTGATAATCTGAATAGGTCCGCAGAatata                                                       <  2:2792340/150‑1 (MQ=255)

                                                                                                ttttttACCAACCCTGGGTCAATGCATTACCGTCAACGCCCCGACACGCAAGTCCTGAGCAATTAGAAAAAACAGTTCGTTATCTTACACAAACTGTGCATCCACGTAGTGCCGACAATATTGATAATCTGAATAGGTCCGCAGAatata                                                       <  2:2162653/150‑1 (MQ=255)

                                                                                                ttttttACCAACCCTGGGTCAATGCATTACCGTCAACGCCCCGACACGCAAGTCCTGAGCAATTAGAAAAAACAGTTCGTTATCTTACACAAACTGTGCATCCACGTAGTGCCGACAATATTGATAATCTGAATAGGTCCGCAGAatata                                                       <  2:3796298/150‑1 (MQ=255)

                                                                                                   tttACCAACCCTGGGTCAATGCATTACCGTCAACGCCCCGACACGCAAGTCCTGAGCAATTAGAAAAAACAGTTCGTTATCTTACACAAACTGTGCATCCACGTAGTGCCGACAATATTGATAATCTGAATAGGTCCGCAGAATATATaa                                                    <  1:3944325/150‑1 (MQ=255)

                                                                                                     tACCAACCCTGGGTCAATGCATTACCGTCAACGCCCCGACACGCAAGTCCTGAGCAATTAGAAAAAACAGTTCGTTATCTTACACAAACTGTGCATCCACGTAGTGCCGACAATATTGATAATCTGAATAGGTCCGCAGAATATATaaaa                                                  >  1:2294313/1‑150 (MQ=255)

                                                                                                      aCCAACCCTGGGTCAATGCATTACCGTCAACGCCCCGACACGCAAGTCCTGAGCAATTAGAAAAAACAGTTCGTTATCTTACACAAACTGTGCATCCACGTAGTGCCGACAATATTGATAATCTGAATAGGTCCGCAGAATATATAAAag                                                 >  1:3457775/1‑150 (MQ=255)

                                                                                                         aaCCCTGGGTCAATGCATTACCGTCAACGCCCCGACACGCAAGTCCTGAGCAATTAGAAAAAACAGTTCGTTATCTTACACAAACTGTGCATCCACGTAGTGCCGACAATATTGATAATCTGAATAGGTCCGCAGAATATATAAAAGAgg                                              <  1:1720554/150‑1 (MQ=255)

                                                                                                           cccTGGGTCAATGCATTACCGTCAACGCCCCGACACGCAAGTCCTGAGCAATTAGAAAAAACAGTTCGTTATCTTACACAAACTGTGCATCCACGTAGTGCCGACAATATTGATAATCTGAATAGGTCCGCAGAATATATAAAAGAGGTc                                            >  2:4850805/1‑150 (MQ=255)

                                                                                                            ccTGGGTCAATGCATTACCGTCAACGCCCCGACACGCAAGTTCTGAGCAATTAGAAAAAACAGTTCGTTATCTTACACAAACTGTGCATCCACGTAGTGCCGACAATATTGATAATCTGAATAGGTCCGCAGAATATATAAAAGAGGTCt                                           <  2:4949170/150‑1 (MQ=255)

                                                                                                            ccTGGGTCAATGCATTACCGTCAACGCCCCGACACGCAAGTCCTGAGCAATTAGAAAAAACAGTTCGTTATCTTACACAAACTGTGCATCCACGTAGTGCCGACAATATTGATAATCTGAATAGGTCCGCAGAATATATAAAAGAGGTCt                                           <  1:4159685/150‑1 (MQ=255)

                                                                                                            ccTGGGTCAATGCATTACCGTCAACGCCCCGACACGCAAGTCCTGAGCAATTAGAAAAAACAGTTCGTTATCTTACACAAACTGTGCATCCACGTAGTGCCGACAATATTGATAATCTGAATAGGTCCGCAGAATATATAAAAGAGGTCt                                           <  1:2966727/150‑1 (MQ=255)

                                                                                                              tGGGTCAATGCATTACCGTCAACGCCCCGACACTCAAGTCCTGAGCAATTAGAAAAAACAGTTCGTTATCTTAAACAAACTGTGCATCCACGTAGTGCCGAAAATATTTATAATCTGAATAGGTCCGCAGAATATATAAAAGAGGTCttt                                         <  1:2207741/150‑1 (MQ=255)

                                                                                                               gggTCAATGCATTACCGTCACCGCCCCGACACGCAAGTCCTGAGCAATTAGAAAAAACAGTTCGTTATCTTACACAAACTGTGCATCCACGTAGTGCCGACAATATTGATAATCTGAATAGGTCCGCAGAATATATAAAAGAGGTCTTTg                                        <  2:894325/150‑1 (MQ=255)

                                                                                                                  tCAATGCATTACCGTCAACGCCCCGACACGCAAGTCCTGAGCAATTAGAAAAAACAGTTCGTTATCTTACACAAACTGTGCATCCACGTAGTGCCGACAATATTGATAATCTGAATAGGTCCGCAGAATATATAAAAGAGGTCTTTGTCa                                     <  2:4487587/150‑1 (MQ=255)

                                                                                                                    aaTGCATTACCGTCAACGCCCCGACACGCAAGTCCTGAGCAATTAGAAAAAACAGTTCGTTATCTTACACAAACTGTGCATCCACGTAGTGCCGACAATATTGATAATCTGAATAGGTCCGCAGAATATATAAAAGAGGTCTTTGTCAGt                                   >  2:752642/1‑150 (MQ=255)

                                                                                                                    aaTGCATTACCGTCAACGCCCCGACACGCAAGTCCTGAGCAATTAGAAAAAACAGTTCGTTATCTTACACAAACTGTGCATCCACGTAGTGCCGACAATATTGATAATCTGAAGAGGTCCGCAGAATATATAAAAGAGGTCTTTGTCAGt                                   >  2:3256748/1‑150 (MQ=255)

                                                                                                                      tGCATTACCGTCAACGCCCCGACACGCAAGTCCTGAGCAATTAGAAAAAACAGTTCGTTATCTTACACAAACTGTGCATCCACGTAGTGCCGACAATATTGATAATCTGAATAGGTCCGCAGAATATATAAAAGAGGTCTTTGTCAGTAg                                 >  1:3257670/1‑150 (MQ=255)

                                                                                                                      tGCATTACCGTCAACGCCCCGACACGCAAGTCCTGAGCAATTAGAAAAAACAGTTCGTTATCTTACACAAACTGTGCATCCACGTAGTGCCGACAATATTGATAATCTGAATAGGTCCGCAGAATATATAAAAGAGGTCTTTGTCAGTAg                                 <  2:4732682/150‑1 (MQ=255)

                                                                                                                          ttACCGTCAACGCCCCGACACGCAAGTCCTGAGCAATTAGAAAAAACAGTTCGTTATCTTACACAAACTGTGCATCCACGTAGTGCCGACAATATTGATAATCTGAATAGGTCCGCAGAATATATAAAAGAGGTCTTTGTCAGTAGCGGt                             <  1:3343652/150‑1 (MQ=255)

                                                                                                                          ttACCGTCAACGCCCCGACACGCAAGTCCTGAGCAATTAGAAAAAACAGTTCGTTATCTTACACAAACTGTGCATCCACGTAGTGCCGACAATATTGATAATCTGAATAGGTCCGCAGAATATATAAAAGAGGTCTTTGTCAGTAGCGGt                             <  1:4035836/150‑1 (MQ=255)

                                                                                                                            aCCGTCAACGCCCCGACACGCAAGTCCTGAGCAATTAGAAAAAACAGTTCGTTATCTTACACAAACTGTGCATCCACGTAGTGCCGACAATATTGATAATCTGAATAGGTCCGCAGAATATATAAAAGAGGTCTTTGTCAGTAGCGGTGc                           >  1:5067673/1‑150 (MQ=255)

                                                                                                                              cgacAACGCCCCGACACGCAAGTCCTGAGCAATTAGAAAAAACAGTTCGTTATCTTACACAAACTGTGCATCCACGTAGTGCCGACAATATTGATAATCTGAATAGGTCCGCAGAATATATAAAAGAGGTCTTTGTCAGTAGCGGTGCCa                         <  2:2551889/147‑1 (MQ=255)

                                                                                                                                 cAACGCCCCGACACGCAAGTCCTGAGCAATTAGAAAAAACAGTTCGTTATCTTACACAAACTGTGCATCCACGTAGTGCCGACAATATTGATAATCTGAATAGGTCCGCAGAATATATAAAAGAGGTCTTTGTCAGTAGCGGTGCCagag                      <  2:1885016/150‑1 (MQ=255)

                                                                                                                                      ccccGACACGCAAGTCCTGAGCAATTAGAAAAAACAGTTCGTTATCTTACACAAACTGTGCATCCACGTAGTGCCGACAATATTGATAATCTGAATAGGTCCGCAGAATATATAAAAGAGGTCTTTGTCAGTAGCGGTGCCAGAGTTAcc                 <  1:3770641/150‑1 (MQ=255)

                                                                                                                                       cccGACACGCAAGTTCTGAGCAATTAGAAAAAACAGTTCGTTATCTTACACAAACTGTGCATCCACGTAGTGCCGACAATATTGATAATCTGAATAGGTCCGCAGAATATATAAAAGAGGTCTTTGTCAGTAGCGGTGCCAGAGTTACCt                >  1:553266/1‑150 (MQ=255)

                                                                                                                                         cGACACGCAAGTCCTGAGCAATTAGAAAAAACAGTTCGTTATCTTACACAAACTGTGCATCCACGTAGTGCCGACAATATTGATAATCTGAATAGGTCCGCAGAATATATAAAAGAGGTCTTTGTCAGTAGCGGTGCCAGAGTTACCTCg              >  1:1537528/1‑150 (MQ=255)

                                                                                                                                         cGACACGCAAGTCCTGAGCAATTAGAAAAAACAGTTCGTTATCTTACACAAACTGTGCATCCACGTAGTGCCGACAATATTGATAATCTGAATAGGTCCGCAGAATATATAAAAGAGGTCTTTGTCAGTAGCGGTGCCAGAGTTACCTCg              >  2:939429/1‑150 (MQ=255)

                                                                                                                                         cGACACGCAAGTCCTGAGCAATTAGAAAAAACAGTTCGTTATCTTACACAAACTGTGCATCCACGTAGTGCCGACAATATTGATAATCTGAATAGGTCCGCAGAATATATAAAAGAGGTCTTTGTCAGTAGCGGTGCCAGAGTTACCTCg              >  2:1233258/1‑150 (MQ=255)

                                                                                                                                          gACACGCAAGTCCTGAGCAATTAGAAAAAACAGTTCGTTATCTTACACAAACTGTGCATCCACGTAGTGCCGACAATATTGATAATCTGAATAGGTCCGCAGAATATATAAAAGAGGTCTTTGTCAGTAGCGGTGCCAGAGTTACCTCGc             >  1:925190/1‑150 (MQ=255)

                                                                                                                                               gCAAGTTCTGAGCAATTAGAAAAAACAGTTCGTTATCTTACACAAACTGTGCATCCACGTAGTGCCGACAATATTGATAATCTGAATAGGTCCGCAGAATATATAAAAGAGGTCTTTGTCAGTAGCGGTGCCAGAGTTACCTCGCAGGAc        <  2:4039206/150‑1 (MQ=255)

                                                                                                                                               gCAAGTCCTGAGCAATTAGAAAAAACAGTTCGTTATCTTACACAAACTGTGCATCCACGTAGTGCCGACAATATTGATAATCTGAATAGGTCCGCAGAATATATAAAAGAGGTCTTTGTCAGTAGCGGTGCCAGAGTTACCTCGCAGGAc        >  1:1085436/1‑150 (MQ=255)

                                                                                                                                                  aGTCCTGAGCAATTAGAAAAAACAGTTCGTTATCTTACACAAACTGTGCATCCACGTAGTGCCGACAATATTGATAATCTGAATAGGTCCGCAGAATATATAAAAGAGGTCTTTGTCAGTAGCGGTGCCAGAGTTACCTCGCAGGACGTc     >  2:2466967/1‑150 (MQ=255)

ATAATGATTCAACAACGCAACCCATTAATAATTTGCATATGAAAAAAATAATCTTTGCTTTTATTATATTATTTGTGTTTTTACTCCCTATGATTATTTTTTACCAACCCTGGGTCAATGCATTACCGTCAACGCCCCGACACGCAAGTCCTGAGCAATTAGAAAAAACAGTTCGTTATCTTACACAAACTGTGCATCCACGTAGTGCCGACAATATTGATAATCTGAATAGGTCCGCAGAATATATAAAAGAGGTCTTTGTCAGTAGCGGTGCCAGAGTTACCTCGCAGGACGTCCCC  >  CP009273/2379301‑2379599

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`