BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	2,412,205	C→A	43.5%	D151Y (GAT→TAT)	yfcD ←	putative NUDIX hydrolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	2,412,205	0	C	A	43.5%	320.4 / inf	351	D151Y (GAT→TAT)	yfcD	putative NUDIX hydrolase
Reads supporting (aligned to +/- strand): ref base C (112/86); new base A (86/67); total (198/153)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 351 total aligned reads displayed.

GCTCAGTTGATGGGCTACCACTAAAAATGTTAAGAGCCGCAATTGCGGCTCTTTTTTCATTCTGCCGTTTCAGTCTCTACGGCTTCATTTTTGGCATTGCGCTTCATCCACAACGCTAGCGCTTTCAGCGAGTCTGGAGTGAACTCATCGCAGCGTGCGGTGATTTCTTCCGGCGTCAGCCAGCAAACTTCACTGACTTCATCTTCCTGTAGTGCGAAGGGACCGTGAGAGACGCAGCTGAACAATGCGCCCCAGACACGGCAATTTTTATCTTCGAAATAGAACTGCCCGTGCTCG  >  CP009273/2412057‑2412353

       tGATGGGCTCCCACTAAAAATGTTAAGAGCCGCAATTGCGGCTCTTTTTTCATTCTGCCGTTTCAGTCTCTACGGCTTCATTTTTGGCATTGCGCTTCATCCACAACGCTAGCGCTTTCAGCGAGTCTGGAGTGAACTCATCGCAGCGTg                                                                                                                                              <  1:3700348/150‑1 (MQ=255)

       tGATGGGCTACCACTAAAAATGTTAAGAGCCGCAATTGCGGCTCTTTTTTCATTCTGCCGTTTCAGTCTCTACGGCTTCATTTTTGGCATTGCGCTTCATCCACAACGCTAGCGCTTTCAGCGAGTCTGGAGTGAACTCATCGCAGCGTg                                                                                                                                              <  2:2403356/150‑1 (MQ=255)

       tGATGGGCTACCACTAAAAATGTTAAGAGCCGCAATTGCGGCTCTTTTTTCATTCTGCCGTTTCAGTCTCTACGGCTTCATTTTTGGCATTGCGCTTCATCCACAACGCTAGCGCTTTCAGCGAGTCTGGAGTGAACTCATCGCAGCGTg                                                                                                                                              >  1:3774225/1‑150 (MQ=255)

          tGGGCTACCACTAAAAATGTTAAGAGCCGCAATTGCGGCTCTTTTTTCATTCTGCCGTTTCAGTCTCTACGGCTTCATTTTTGGCATTGCGCTTCATCCACAACGCTAGCGCTTTCAGCGAGTCTGGAGTGAACTCATAGCAGCGTGCgg                                                                                                                                           >  1:4410007/1‑150 (MQ=255)

          tGGGCTACCACTAAAAATGTTAAGAGCCGCAATTGCGGCTCTTTTTTCATTCTGCCGTTTCAGTCTCTACGGCTTCATTTTTGGCATTGCGCTTCATCCACAACGCTAGCGCTTTCAGCGAGTCTGGAGTGAACTCATAGCAGCGTGCgg                                                                                                                                           <  2:2444468/150‑1 (MQ=255)

             gCTACCACTAAAAATGTTAAGAGCCGCAATTGCGGCTCTTTTTTCATTCTGCCGTTTCAGTCTCTACGGCTTCATTTTTGGCATTGCGCTTCATCCACAACGCTAGCGCTTTAAGCGAGTCTGGAGTGAACTCATCGCAGCGTGCGGTGa                                                                                                                                        >  1:1369017/1‑150 (MQ=255)

              cTACCACTAAAAATGTTAAGAGCCGCAATTGCGGCTCTTTTTTCATTCTGCCGTTTCAGTCTCTACGGCTTCATTTTTGGCATTGCGCTTCATCCACAACGCTAGCGCTTTCAGCGAGTCTGGAGTGAACTCATCGCAGCGTGCGGTGAt                                                                                                                                       >  2:573292/1‑150 (MQ=255)

              cTACCACTAAAAATGTTAAGAGCCGCAATTGCGGCTCTTTTTTCATTCTGCCGTTTCAGTCTCTACGGCTTCATTTTTGGCATTGCGCTTCATCCACAACGCTAGCGCTTTCAGCGAGTCTGGAGTGAACTCATCGCAGCGTGCGGTGAt                                                                                                                                       >  2:4366531/1‑150 (MQ=255)

              cTACCACTAAAAATGTTAAGAGCCGCAATTGCGGCTCTTTTTTCATTCTGCCGTTTCAGTCTCTACGGCTTCATTTTTGGCATTGCGCTTCATCCACAACGCTAGCGCTTTCAGCGAGTCTGGAGTGAACTCATAGCATCGTGCGGTGAt                                                                                                                                       >  2:1383245/1‑150 (MQ=255)

               tACCACTAAAAATGTTAAGAGCCGCAATTGCGGCTCTTTTTTCATTCTGCCGTTTCAGTCTCTACGGCTTCATTTTTGGCATTGCGCTTCATCCACAACGCTAGCGCTTTCAGCGAGTCTGGAGTGAACTCATCGCAGCGTGCGGTGAtt                                                                                                                                      >  2:3087369/1‑150 (MQ=255)

                aCCACTAAAAATGTTAAGAGCCGCAAGTGCGGCTCTTTTTTCATTCTGCCGTTTCAGTCTCTACGGCTTCATTTTTGGCATTGCGCTTCATCCACAACGCTAGCGCTTTCAGCGAGTCTGGAGTGAACTCATCGCAGCGTGCGGTGAttt                                                                                                                                     <  2:2937949/150‑1 (MQ=255)

                   aCTAAAAATGTTAAGAGCCGCAATTGCGGCTCTTTTTTCATTCTGCCGTTTCAGTCTCTACGGCTTCATTTTTGGCATTGCGCTTCATCCACAACGCTAGCGCTTTCAGCGAGTCTGGAGTGAACTCATCGCAGCGTGCGGTGATttctt                                                                                                                                  >  2:1838610/1‑150 (MQ=255)

                     tAAAAATGTTAAGAGCCGCAATTGCGGCTCTTTTTTCATTCTGCCGTTTCAGTCTCTACGGCTTCATTTTTGGCATTGCGCTTCATCCACAACGCTAGCGCTTTCAGCGAGTCTGGAGTGAACTCATCGCAGCGTGCGGTGATTTCTTcc                                                                                                                                >  2:946510/1‑150 (MQ=255)

                      aaaaaTGTTAAGAGCCGCAATTGCGGCTCTTTTTTCATTCTGCCGTTTCAGTCTCTACGGCTTCATTTTTGGCATTGCGCTTCATCCACAACGCTAGCGCTTTCAGCGAGTCTGGAGTGAACTCATCGCAGCGTGCGGTGATTTCTTCCg                                                                                                                               <  2:1936440/150‑1 (MQ=255)

                      aaaaaTGTTAAGAGCCGCAATTGCGGCTCTTTTTTCATTCTGCCGTTTCAGTCTCTACGGCTTCATTTTTGGCATTGCGCTTCATCCACAACGCTAGCGCTTTCAGCGAGTCTGGAGTGAACTCATAGCAGCGTGCGGTGATTTCTTCCg                                                                                                                               >  2:2121733/1‑150 (MQ=255)

                       aaaaTGTTAAGAGCCGCAATTGCGGCTCTTTTTTCATTCTGCCGTTTCAGTCTCTACGGCTTCATTTTTGGCATTGCGCTTCATCCACAACGCTAGCGCTTTCAGCGAGTCTGGAGTGAACTCATCGCAGCGTGCGGTGATTTCTTCCgg                                                                                                                              >  1:727294/1‑150 (MQ=255)

                          aTGTTAAGAGCCGCAATTGCGGCTCTTTTTTCATTCTGCCGTTTCAGTCTCTACGGCTTCATTTTTGGCATTGCGCTTCATCCACAACGCTAGCGCTTTCAGCGAGTCTGGAGTGAACTCATCGCAGCGTGCGGTGATTTCTTCCGGCGt                                                                                                                           >  1:321562/1‑150 (MQ=255)

                           tGTTAAGAGCCGCAATTGCGGCTCTTTTTTCATTCTGCCGTTTCAGTCTCTACGGCTTCATTTTTGGCATTGCGCTTCATCCACAACGCTAGCGCTTTCAGCGAGTCTGGAGTGAACTCATAGCAGCGTGCGGTGATTTCTTCCGGCGTc                                                                                                                          <  1:4095935/150‑1 (MQ=255)

                            gTTAAGAGCCGCAATTGCGGCTCTTTTTTCATTCTGCCGTTTCAGTCTCTACGGCTTCATTTTTGGCATTGCGCTTCATCCACAACGCTAGCGCTTTCAGCGAGTCTGGAGTGAACTCATCGCAGCGTGCGGTGATTTCTTCCGGCGTca                                                                                                                         <  2:1246502/150‑1 (MQ=255)

                            gTTAAGAGCCGCAATTGCGGCTCTTTTTTCATTCTGCCGTTTCAGTCTCTACGGCTTCATTTTTGGCATTGCGCTTCATCCACAACGCTAGCGCTTTCAGCGAGTCTGGAGTGAACTCATCGCAGCGTGCGGTGATTTCTTCCGGCGTca                                                                                                                         >  1:1017940/1‑150 (MQ=255)

                            gTTAAGAGCCGCAATTGCGGCTCTTTTTTCATTCTGCCGTTTCAGTCTCTACGGCTTCATTTTTGGCATTGCGCTTCATCCACAACGCTAGCGCTTTCAGCGAGTCTGGAGTGAACTCATAGCAGCGTGCGGTGATTTCTTCCGGCGTca                                                                                                                         >  2:1669925/1‑150 (MQ=255)

                            gTTAAGAGCCGCAATTGCGGCTCTTTTTTCATTCTGCCGTTTCAGTCTCTACGGCTTCATTTTTGGCATTGCGCTTCATCCACAACGCTAGCGCTTTCAGCGAGTCTGGAGTGAACTCATAGCAGCGTGCGGTGATTTCTTCCGGCGTca                                                                                                                         <  2:1185847/150‑1 (MQ=255)

                              tAAGAGCCGCAATTGCGGCTCTTTTTTCATTCTGCCGTTTCAGTCTCTACGGCTTCATTTTTGGCATTGCGCTTCATCCACAACGCTAGCGCTTTCAGCGAGTCTGGAGTGAACTCATAGCAGCGTGCGGTGATTTCTTCCGGCGTcagc                                                                                                                       >  1:4711287/1‑150 (MQ=255)

                               aaGAGCCGCAATTGCGGCTCTTTTTTCATTCTGCCGTTTCAGTCTCTACGGCTTCATTTTTGGCATTGCGCTTCATCCACAACGCTAGCGCTTTCAGCGAGTCTGGAGTGAACTCATAGCAGCGTGCGGTGATTTCTTCCGGCGTcagcc                                                                                                                      >  1:851530/1‑150 (MQ=255)

                                tgagCGGCAATTGCGGCTCTTTTTTCATTCTTCCGTTTCAGTCTCTACGGCTTCATTTTTGGCATTGCGCTTCATCCACAACGCTAGCGCTTTCAGCGAGTCTGGAGTGAACTCATCGCAGCGTGCGGTGATTTCTTCCGGCGTcagcca                                                                                                                     <  1:2193787/149‑1 (MQ=255)

                                   gCCGCAATTGCGGCTCTTTTTTCATTCTGCCGTTTCAGTCTCTACGGCTTCATTTTTGGCATTGCGCTTCATCCACAACGCTAGCGCTTTCAGCGAGTCTGGAGTGAACTCATAGCAGCGTGCGGTGATTTCTTCCGGCGTCAGCCAGCa                                                                                                                  <  1:1751028/150‑1 (MQ=255)

                                     cGCAATTGCGGCTCTTTTTTCATTCTGCCGTTTCAGTCTCTACGGCTTCATTTTTGGCATTGCGCTTCATCCACAACGCTAGCGCTTTCAGCGAGTCTGGAGTGAACTCATAGCAGCGTGCGGTGATTTCTTCCGGCGTCAGCCAGCaaa                                                                                                                >  1:753403/1‑150 (MQ=255)

                                      gCAATTGCGGCTCTTTTTTCATTCTGCCGTTTCAGTCTCTACGGCTTCATTTTTGGCATTGCGCTTCATCCACAACGCTAGCGCTTTCAGCGAGTCTGGAGTGAACTCATCGCAGCGTGCGGTGATTTCTTCCGGCGTCAGCCAGCAAAc                                                                                                               >  1:5076962/1‑150 (MQ=255)

                                       cAATTGCGGCTCTTTTTTCATTCTGCCGTTTCAGTCTCTACGGCTTCATTTTTGGCATTGCGCTTCATCCACAACGCTAGCGCTTTCAGCGAGTCTGGAGTGAACTCATCGCAGCGTGCGGTGATTTCTTCCGGCGTCAGCCAGCAAACt                                                                                                              >  1:4821843/1‑150 (MQ=255)

                                       cAATTGCGGCTCTTTTTTCATTCTGCCGTTTCAGTCTCTACGGCTTCATTTTTGGCATTGCGCTTCATCCACAACGCTAGCGCTTTCAGCGAGTCTGGAGTGAACTCATAGCAGCGTGCGGTGATTTCTTCCGGCGTCAGCCAGCAAACt                                                                                                              >  1:3431179/1‑150 (MQ=255)

                                        aaTTGCGGCTCTTTTTTCATTCTGCCGTTTCAGTCTCTACGGCTTCATTTTTGGCATTGCGCTTCATCCACAACGCTAGCGCTTTCAGCGAGTCTGGAGTGAACTCATCGCAGCGTGCGGTGATTTCTTCCGGCGTCAGCCAGCAAACtt                                                                                                             >  2:2880593/1‑150 (MQ=255)

                                        aaTTGCGGCTCTTTTTTCATTCTGCCGTTTCAGTCTCTACGGCTTCATTTTTGGCATTGCGCTTCATCCACAACGCTAGCGCTTTCAGCGAGTCTGGAGTGAACTCATCGCAGCGTGCGGTGATTTCTTCCGGCGTCAGCCAGCAAACtt                                                                                                             <  2:3455410/150‑1 (MQ=255)

                                        aaTTGCGGCTCTTTTTTCATTCTGCCGTTTCAGTCTCTACGGCTTCATTTTTGGCATTGCGCTTCATCCACAACGCTAGCGCTTTCAGCGAGTCTGGAGTGAACTCATAGCAGCGTGCGGTGATTTCTTCCGGCGTCAGCCAGCAAACtt                                                                                                             <  2:3666978/150‑1 (MQ=255)

                                              ggCTCTTTTTTCATTCTGCCGTTTCAGTCTCTACGGCTTCATTTTTGGCATTGCGCTTCATCCACAACGCTAGCGCTTTCAGCGAGTCTGGAGTGAACTCATCGCAGCGTGCGGTGATTTCTTCCGGCGTCAGCCAGCAAACTTCACTGa                                                                                                       >  2:4065167/1‑150 (MQ=255)

                                              ggCTCTTTTTTCATTCTGCCGTTTCAGTCTCTACGGCTTCATTTTTGGCATTGCGCTTCATCCACAACGCTAGCGCTTTCAGCGAGTCTGGAGTGAACTCATCGCAGCGTGCGGTGATTTCTTCCGGCGTCAGCCAGCAAACTTCACTGa                                                                                                       >  1:509696/1‑150 (MQ=255)

                                              ggCTCTTTTTTCATTCTGCCGTTTCAGTCTCTACGGCTTCATTTTTGGCATTGCGCTTCATCCACAACGCTAGCGCTTTCAGCGAGTCTGGAGTGAACTCATCGCAGCGTGCGGTGATTTCTTCCGGCGTCAGCCAGCAAACTTCACTGa                                                                                                       >  1:1724709/1‑150 (MQ=255)

                                                ctctTTTTTCATTCTGCCGTTTCAGTCTCTACGGCTTCATTTTTGGCATTGCGCTTCATCCACAACGCTAGCGCTTTCAGCGAGTCTGGAGTGAACTCATAGCAGCGTGCGGTGATTTCTTCCGGCGTCAGCCAGCAAACTTCACTGACt                                                                                                     <  2:2189384/150‑1 (MQ=255)

                                                 tctTTTTTCATTCTGCCGTTTCAGTCTCTACGGCTTCATTTTTGGCATTGCGCTTCATCCACAACGCTAGCGCTTTCAGCGAGTCTGGAGTGAACTCATCGCAGCGTGCGGTGATTTCTTCCGGCGTCAGCCAGCAAACTTCACTGACtt                                                                                                    >  2:1107651/1‑150 (MQ=255)

                                                     ttttCATTCTGCCGTTTCAGTCTCTACGGCTTCATTTTTGGCATTGCGCTTCATCCACAACGCTAGCGCTTTCAGCGAGTCTGGAGTGAACTCATCGCAGCGTGCGGTGATTTCTTCCGGCGTCAGCCAGCAAACTTCACTGACTTCATc                                                                                                >  1:891263/1‑150 (MQ=255)

                                                      tttCATTCTGCCGTTTCAGTCTCTACGGCTTCATTTTTGGCATTGCGCTTCATCCACAACGCTAGCGCTTTCAGCGAGTCTGGAGTGAACTCATCGCAGCGTGCGGTGATTTCTTCCGGCGTCAGCCAGCAAACTTCACTGACTTCATCt                                                                                               >  1:3336179/1‑150 (MQ=255)

                                                       ttCATTCTGCCGTTTCAGTCTCTACGGCTTCATTTTTGGCATTGCGCTTCATCCACAACGCTAGCGCTTTCAGCGAGTCTGGAGTGAACTCATAGCAGCGTGCGGTGATTTCTTCCGGCGTCAGCCAGCAAACTTCACTGACTTCATCtt                                                                                              >  1:946566/1‑150 (MQ=255)

                                                        tCATTCTGCCGTTTCAGTCTCTACGGCTTCATTTTTGGCATTGCGCTTCATCCACAACGCTAGCGCTTTCAGCGAGTCTGGAGTGAACTCATCGCAGCGTGCGGTGATTTCTTCCGGCGTCAGCCAGCAAACTTCACTGACTTCATCTTc                                                                                             <  2:1469038/150‑1 (MQ=255)

                                                         cATTCTGCCGTTTCAGTCTCTACGGCTTCATTTTTGGCATTGCGCTTCATCCACAACGCTAGCGCTTTCAGCGAGTCTGGAGTGAACTCATCGCAGCGTGCGGTGATTTCTTCCGGCGTCAGCCAGCAAACTTCACTGACTTCATCTTcc                                                                                            <  1:378877/150‑1 (MQ=255)

                                                          aTTCTGCCGTTTCAGTCTCTACGGCTTCATTTTTGGCATTGCGCTTCATCCACAACGCTAGCGCTTTCAGCGAGTCTGGAGTGAACTCATCGCAGCGTGCGGTGATTTCTTCCGGCGTCAGCCAGCAAACTTCACTGACTTCATCTTCCt                                                                                           >  1:4651075/1‑150 (MQ=255)

                                                             cTGCCGTTTCAGTCTCTCCGGCTTCATTTTTGGCATTGCGCTTCATCCACAACGCTAGCGCTTTCAGCGAGTCTGGAGTGAACTCATCGCAGCGTGCGGTGATTTCTTCCGGCGTCAGCCAGCAAACTTCACTGACTTCATCTTCCTGTa                                                                                        <  2:700911/150‑1 (MQ=255)

                                                              tGCCGTTTCAGTCTCTACGGCTTCATTTTTGGCATTGCGCTTCATCCACAACGCTAGCGCTTTCAGCGAGTCTGGAGTGAACTCATCGCAGCGTGCGGTGATTTCTTCCGGCGTCAGCCAGCAAACTTCACTGACTTCATCTTCCTGTAg                                                                                       <  2:968588/150‑1 (MQ=255)

                                                              tGCCGTTTCAGTCTCTACGGCTTCATTTTTGGCATTGCGCTTCATCCACAACGCTAGCGCTTTCAGCGAGTCTGGAGTGAACTCATCGCAGCGTGCGGTGATTTCTTCCGGCGTCAGCCAGCAAACTTCACTGACTTCATCTTCCTGTAg                                                                                       >  1:3017269/1‑150 (MQ=255)

                                                              tGCCGTTTCAGTCTCTACGGCTTCATTTTTGGCATTGCGCTTCATCCACAACGCTAGCGCTTTCAGCGAGTCTGGAGTGAACTCATCGCAGCGTGCGGTGATTTCTTCCGGCGTCAGCCAGCAAACTTCACTGACTTCATCTTCCTGTAg                                                                                       >  1:1226691/1‑150 (MQ=255)

                                                                  gTTTCAGTCTCTACGGCTTCATTTTTGGCATTGCGCTTCATCCACAACGCTAGCGCTTTCAGCGAGTCTGGAGTGAACTCATCGCAGCGTGCGGTGATTTCTTCCGGCGTCAGCCAGCAAACTTCACTGACTTCATCTTCCTGTAGTGCg                                                                                   >  1:2471580/1‑150 (MQ=255)

                                                                   tttCAGTCTCTACGGCTTCATTTTTGGCATTGCGCTTCATCCACAACGCTAGCGCTTTCAGCGAGTCTGGAGTGAACTCATCGCAGCGTGCGGTGATTTCTTCCGGCGTCAGCCAGCAAACTTCACTGACTTCATCTTCCTGTAGTGCGa                                                                                  <  1:1516018/150‑1 (MQ=255)

                                                                     tCAGTCTCTACGGCTTCATTTTTGGCATTGCGCTTCATCCACAACGCTAGCGCTTTCAGCGAGTCTGGAGTGAACTCATCGCAGCGTGCGGTGATTTCTTCCGGCGTCAGCCAGCAAACTTCACTGACTTCATCTTCCTGTAGTGCGAAg                                                                                >  2:928334/1‑150 (MQ=255)

                                                                     tCAGTCTCTACGGCTTCATTTTTGGCATTGCGCTTCATCCACAACGCTAGCGCTTTCAGCGAGTCTGGAGTGAACTCATAGCAGCGTGCGGTGATTTCTTCCGGCGTCAGCCAGCAAACTTCACTGACTTCATCTTCCTGTAGTGCGAAg                                                                                >  1:1989643/1‑150 (MQ=255)

                                                                        gtatctACGGCTTCATTTTTGGCATTGCGCTTCATCCACAACGCTAGCGCTTTCAGCGAGTCTGGAGTGAACTCATATCAGCGTGCGGTGATTTCTTCCGGCGTCAGCCAGCAAACTTCACTGACTTCATCTTCCTGTAGTGCGAAGGGa                                                                             >  2:4761290/4‑150 (MQ=255)

                                                                          ctctACGGCTTCATTTTTGGCATTGCGCTTCATCCACAACGCTAGCGCTTTCAGCGAGTCTGGAGTGAACTCATCGCAGCGTGCGGTGATTTCTTCCGGCGTCAGCCAGCAAACTTCACTGACTTCATCTTCCTGTAGTGCGAAGGGAcc                                                                           >  1:906836/1‑150 (MQ=255)

                                                                          ctctACGGCTTCATTTTTGGCATTGCGCTTCATCCACAACGCTAGCGCTTTCAGCGAGTCTGGAGTGAACTCATCGCAGCGTGCGGTGATTTCTTCCGGCGTCAGCCAGCAAACTTCACTGACTTCATCTTCCTGTAGTGCGAAGGGAcc                                                                           >  2:763395/1‑150 (MQ=255)

                                                                           tctACGGCTTCATTTTTGGCATTGCGCTTCATCCACAACGCTAGCGCTTTCAGCGAGTCTGGAGTGAACTCATAGCAGCGTGCGGTGATTTCTTCCGGCGTCAGCCAGCAAACTTCACTGACTTCATCTTCCTGTAGTGCGAAGGGACCg                                                                          <  2:3528910/150‑1 (MQ=255)

                                                                            ctACGGCTTCATTTTTGGCATTGCGCTTCATCCACAACGCTAGCGCTTTCAGCGAGTCTGGAGTGAACTCATCGCAGCGTGCGGTGATTTCTTCCGGCGTCAGCCAGCAAACTTCACTGACTTCATCTTCCTGTAGTGCGAAGGGACCGt                                                                         >  2:82926/1‑150 (MQ=255)

                                                                            ctACGGCTTCATTTTTGGCATTGCGCTTCATCCACAACGCTAGCGCTTTCAGCGAGTCTGGAGTGAACTCATAGCAGCGTGCGGTGATTTCTTCCGGCGTCAGCCAGCAAACTTCACTGACTTCATCTTCCTGTAGTGCGAAGGGACCGt                                                                         >  1:2820703/1‑150 (MQ=255)

                                                                             tACGGCTTCATTTTTGGCATTGCGCTTCATCCACAACGCTAGCGCTTTCAGCGAGTCTGGAGTGAACTCATAGCAGCGTGCGGTGATTTCTTCCGGCGTCAGCCAGCAAACTTCACTGACTTCATCTTCCTGTAGTGCGAAGGGACCGTg                                                                        >  1:4363525/1‑150 (MQ=255)

                                                                               cGGCTTCATTTTTGGCATTGCGCTTCATCCACAACGCTAGCGCTTTCCGCGAGTCTGGAGTGAACTCATCGCAGCGTGCGGTGATTTCTTCCGGCGTCAGCCAGCAAACTTCACTGACTTCATCTTCCTGTAGTGCGAAGGGACCGTgag                                                                      <  1:3420457/150‑1 (MQ=255)

                                                                               cGGCTTCATTTTTGGCATTGCGCTTCATCCACAACGCTAGCGCTTTCAGCGAGTCTGGAGTGAACTCATAGCAGCGTGCGGTGATTTCTTCCGGCGTCAGCCAGCAAACTTCACTGACTTCATCTTCCTGTAGTGCGAAGGGACCGTgag                                                                      <  1:597981/150‑1 (MQ=255)

                                                                                 gCTTCATTTTTGGCATTGCGCTTCATCCACAACGCTAGCGCTTTCAGCGAGTCTGGAGTGAACTCATCGCAGCGTGCGGTGATTTCTTCCGGCGTCAGCCAGCAAACTTCACTGACTTCATCTTCCTGTAGTGCGAAGGGACCGTgagag                                                                    >  2:5014054/1‑150 (MQ=255)

                                                                                   ttCATTTTTGGCATTGCGCTTCATCCACAACGCTAGCGCTTTCAGCGAGTCTGGAGTGAACTCATAGCAGCGTGCGGTGATTTCTTCCGGCGTCAGCCAGCAAACTTCACTGACTTCATCTTCCTGTAGTGCGAAGGGACCGTGAGAGAc                                                                  <  1:142716/150‑1 (MQ=255)

                                                                                       tttttGGCATTGCGCTTCATCCACAACGCTAGCGCTTTCAGCGAGTCTGGAGTGAACTCATCGCAGCGTGCGGTGATTTCTTCCGGCGTCAGCCAGCAAACTTCACTGACTTCATCTTCCTGTAGTGCGAAGGGACCGTGAGAGACGCAg                                                              <  2:2352194/150‑1 (MQ=255)

                                                                                           tGGCATTGCGCTTCATCCACAACGCTAGCGCTTTCAGCGAGTCTGGAGTGAACTCATAGCAGCGTGCGGTGATTTCTTCCGGCGTCAGCCAGCAAACTTCACTGACTTCATCTTCCTGTAGTGCGAAGGGACCGTGAGAGACGCAGCTGa                                                          >  2:1695636/1‑150 (MQ=255)

                                                                                            ggCATTGCGCTTCATCCACAACGCTAGCGCTTTCAGCGAGTCTGGAGTGAACTCATCGCAGCGTGCGGTGATTTCTTCCGGCGTCAGCCAGCAAACTTCACTGACTTCATCTTCCTGTAGTGCGAAGGGACCGTGAGAGACGCAGCTGaa                                                         >  1:3165203/1‑150 (MQ=255)

                                                                                                ttGCGCTTCATCCACAACGCTAGCGCTTTCAGCGAGTCTGGAGTGAACTCATCGCAGCGTGCGGTGATTTCTTCCGGCGTCAGCCAGCAAACTTCACTGACTTCATCTTCCTGTAGTGCGAAGGGACCGTGAGAGACGCAGCTGAACAAt                                                     >  1:451621/1‑150 (MQ=255)

                                                                                                 tGCGCTTCATCCACAACGCTAGCGCTTTCAGCGAGTCTGGAGTGAACTCATCGCAGCGTGCGGTGATTTCTTCCGGCGTCAGCCAGCAAACTTCACTGACTTCATCTTCCTGTAGTGCGAAGGGACCGTGAGAGACGCAGCTGAACAATg                                                    >  1:3726913/1‑150 (MQ=255)

                                                                                                    gcTTCATCCACAACGCTAGCGCTTTCAGCGAGTCTGGAGTGAACTCATAGCAGCGTGCGGTGATTTCTTCCGGCGTCAGCCAGCAAACTTCACTGACTTCATCTTCCTGTAGTGCGAAGGGACCGTGAGAGACGCAGCTGAACAATgcgc                                                 <  2:297254/150‑1 (MQ=255)

                                                                                                      ttCATCCACAACGCTAGCGCTTTCAGCGAGTCTGGAGTGAACTCATAGCAGCGTGCGGTGATTTCTTCCGGCGTCAGCCAGCAAACTTCACTGACTTCATCTTCCTGTAGTGCGAAGGGACCGTGAGAGACGCAGCTGAACAATGCGccc                                               <  2:3966303/150‑1 (MQ=255)

                                                                                                        cATCCACAACGCTAGCGCTTTCAGCGAGTCTGGAGTGAACTCATAGCAGCGTGCGGTGATTTCTTCCGGCGTCAGCCAGCAAACTTCACTGACTTCATCTTCCTGTAGTGCGAAGGGACCGTGAGAGACGCAGCTGAACAATGCGCCCCa                                             <  1:1193180/150‑1 (MQ=255)

                                                                                                        cATCCACAACGCTAGCGCTTTCAGCGAGTCTGGAGTGAACTCATAGCAGCGTGCGGTGATTTCTTCCGGCGTCAGCCAGCAAACTTCACTGACTTCATCTTCCTGTAGTGCGAAGGGACCGTGAGAGACGCAGCTGAACAATGCGCCCCa                                             >  2:1012377/1‑150 (MQ=255)

                                                                                                          tCCACAACGCTAGCGCTTTCAGCGAGTCTGGAGTGAACTCATAGCAGCGTGCGGTGATTTCTTCCGGCGTCAGCCAGCAAACTTCACTGACTTCATCTTCCTGTAGTGCGAAGGGACTGTGAGAGACGCAGCTGAACAATGCGCCCCAGa                                           <  2:1654938/150‑1 (MQ=255)

                                                                                                          tCCACAACGCTAGCGCTTTCAGCGAGTCTGGAGTGAACTCATAGCAGCGTGCGGTGATTTCTTCCGGCGTCAGCCAGCAAACTTCACTGACTTCATCTTCCTGTAGTGCGAAGGGACTGTGAGAGACGCAGCTGAACAATGCGCCCCAGa                                           <  2:4216771/150‑1 (MQ=255)

                                                                                                             acaACGCTAGCGCTTTCAGCGAGTCTGGAGTGAACTCATAGCAGCGTGCGGTGATTTCTTCCGGCGTCAGCCAGCAAACTTCACTGACTTCATCTTCCTGTAGTGCGAAGGGACCGTGAGAGACGCAGCTGAACAATGCGCCCCAGacac                                        >  2:3093320/1‑150 (MQ=255)

                                                                                                              caACGCTAGCGCTTTCAGCGAGTCTGGAGTGAACTCATAGCAGCGTGCGGTGATTTCTTCCGGCGTCAGCCAGCAAACTTCACTGACTTCATCTTCCTGTAGTGCGAAGGGACCGTGAGAGACGCAGCTGAACAATGCGCCCCAGACACg                                       <  1:3637221/150‑1 (MQ=255)

                                                                                                                   cTAGCGCTTTCAGCGAGTCTGGAGTGAACTCATCGCAGCGTGCGGTGATTTCTTCCGGCGTCAGCCAGCAAACTTCACTGACTTCATCTTCCTGTAGTGCGAAGGGACCGTGAGAGACGCAGCTGAACAATGCGCCCCAGACACGGCAAt                                  <  1:4936382/150‑1 (MQ=255)

                                                                                                                   cTAGCGCTTTCAGCGAGTCTGGAGTGAACTCATAGCAGCGTGCGGTGATTTCTTCCGGCGTCAGCCAGCAAACTTCACTGACTTCATCTTCCTGTAGTGCGAAGGGACCGTGAGAGACGCAGCTGAACAATGCGCCCCAGACACGGCAAt                                  >  1:2897113/1‑150 (MQ=255)

                                                                                                                    tAGCGCTTTCAGCGAGTCTGGAGTGAACTCATAGCAGCGTGCGGTGATTTCTTCCGGCGTCAGCCAGCAAACTTCACTGACTTCATCTTCCTGTAGTGCGAAGGGACCGTGAGAGACGCAGCTGAACAATGCGCCCCAGACACGGCAAtt                                 >  2:4463689/1‑150 (MQ=255)

                                                                                                                     aGCGCTTTCAGCGAGTCTGGAGTGAACTCATCGCAGCGTGCGGTGATTTCTTCCGGCGTCAGCCAGCAAACTTCACTGACTTCATCTTCCTGTAGTGCGAAGGGACCGTGAGAGACGCAGCTGAACAATGCGCCCCAGACACGGCAAttt                                <  2:2545605/150‑1 (MQ=255)

                                                                                                                      gcgcTTTCAGCGAGTCTGGAGTGAACTCATAGCAGCGTGCGGTGATTTCTTCCGGCGTCAGCCAGCAAACTTCACTGACTTCATCTTCCTGTAGTGCGAAGGGACCGTGAGAGACGCAGCTGAACAATGCGCCCCAGACACGGCAAtttt                               >  1:1112760/1‑150 (MQ=255)

                                                                                                                         cTTTCAGCGAGTCTGGAGTGAACTCATCGCAGCGTGCGGTGATTTCTTCCGGCGTCAGCCAGCAAACTTCACTGACTTCATCTTCCTGTAGTGCGAAGGGACCGTGAGAGACGCAGCTGAACAATGCGCCCCAGACACGGCAATTTTTAt                            >  2:4537172/1‑150 (MQ=255)

                                                                                                                          tttCAGCGAGTCTGGAGTGAACTCATCGCAGCGTGCGGTGATTTCTTCCGGCGTCAGCCAGCAAACTTCACTGACTTCATCTTCCTGTAGTGCGAAGGGACCGTGAGAGACGCAGCTGAACAATGCGCCCCAGACACGGCAATTTTTATc                           >  2:3709658/1‑150 (MQ=255)

                                                                                                                           ttCAGCGAGTCTGGAGTGAACTCATCGCAGCGTGCGGTGATTTCTTCCGGCGTCAGCCAGCAAACTTCACTGACTTCATCTTCCTGTAGTGCGAAGGGACCGTGAGAGACGCAGCTGAACAATGCGCCCCAGACACGGCAATTTTTATCt                          <  2:4913309/150‑1 (MQ=255)

                                                                                                                             cAGCGAGTCTGGAGTGAACTCATCGCAGCGTGCGGTGATTTCTTCCGGCGTCAGCCAGCAAACTTCACTGACTTCATCTTCCTGTAGTGCGAAGGGACCGTGAGAGACGCAGCTGAACAATGCGCCCCAGACACGGCAATTTTTATCTTc                        <  1:3446871/150‑1 (MQ=255)

                                                                                                                              aGCGAGTCAGGAGTGAACTCATCGCAGCGTGCGGTGATTTCTTCCGGCGTCAGCCAGCAAACTTCACTGACTTCATCTTCCTGTAGTGCGAAGGGACCGTGAGAGACGCAGCTGAACAATGCGCCCCAGACACGGCAATTTTTATCTTCg                       >  1:167086/1‑150 (MQ=255)

                                                                                                                                 gAGTCTGGAGTGAACTCATCGCAGCGTGCGGTGATTTCTTCCGGCGTCAGCCAGCAAACTTCACTGACTTCATCTTCCTGTAGTGCGAAGGGACCGTGAGAGACGCAGCTGAACAATGCGCCCCAGACACGGCAATTTTTATCTTCGaaa                    <  1:2153424/150‑1 (MQ=255)

                                                                                                                                 gAGTCTGGAGTGAACTCATCGCAGCGTGCGGTGATTTCTTCCGGCGTCAGCCAGCAAACTTCACTGACTTCATCTTCCTGTAGTGCGAAGGGACCGTGAGAGACGCAGCTGAACAATGCGCCCCAGACACGGCAATTTTTATCTTCGaaa                    <  1:2758528/150‑1 (MQ=255)

                                                                                                                                  aGTCTGGAGTGAACTCATAGCAGCGTGCGGTGATTTCTTCCGGCGTCAGCCAGCAAACTTCACTGACTTCATCTTCCTGTAGTGCGAAGGGACCGTGAGAGACGCAGCTGAACAATGCGCCCCAGACACGGCAATTTTTATCTTCGAAAt                   >  1:4197119/1‑150 (MQ=255)

                                                                                                                                  aGTCTGGAGTGAACTCATAGCAGCGTGCGGTGATTTCTTCCGGCGTCAGCCAGCAAACTTCACTGACTTCATCTTCCTGTAGTGCGAAGGGACCGTGAGAGACGCAGCTGAACAATGCGCCCCAGACACGGCAATTTTTATCTTCGAAAt                   >  1:3118615/1‑150 (MQ=255)

                                                                                                                                   gTCTGGAGTGAACTCATCGCAGCGTGCGGTGATTTCTTCCGGCGTCAGCCAGCAAACTTCACTGACTTCATCTTCCTGTAGTGCGAAGGGACCGTGAGAGACGCAGCTGAACAATGCGCCCCAGACACGGCAATTTTTATCTACGAAATa                  >  2:4563340/1‑150 (MQ=255)

                                                                                                                                   gTCTGGAGTGAACTCATAGCAGCGTGCGGTGATTTCTTCCGGCGTCAGCCAGCAAACTTCACTGACTTCATCTTCCTGTAGTGCGAAGGGACCGTGAGAGACGCAGCTGAACAATGCGCCCCAGACACGGCAATTTTTATCTTCGAAATa                  >  1:2590362/1‑150 (MQ=255)

                                                                                                                                     cTGGAGTGAACTCATAGCAGCGTGCGGTGATTTCTTCCGGCGTCAGCCAGCAAACTTCACTGACTTCATCTTCCTGTAGTGCGAAGGGACCGTGAGAGACGCAGCTGAACAATGCGCCCCAGACACGGCAATTTTTATCTTCGAAATAGa                <  1:2374856/150‑1 (MQ=255)

                                                                                                                                      tGGAGTGAACTCATAGCAGCGTGCGGTGATTTCTTCCGGCGTCAGCCAGCAAACTTCACTGACTTCATCTTCCTGTAGTGCGAAGGGACCGTGAGAGACGCAGCTGAACAATGCGCCCCAGACACGGCAATTTTTATCTTCGAAATAGaa               >  2:3846676/1‑150 (MQ=255)

                                                                                                                                        gAGTGAACTCATCGCAGCGTGCGGTGATTTCTTCCGGCGTCAGCCAGCAAACTTCACTGACTTCATCTTCCTGTAGTGCGAAGGGACCGTGAGAGACGCAGCTGAACAATGCGCCCCAGACACGGCAATTTTTATCTTCGAAATAGAACt             <  2:3180809/150‑1 (MQ=255)

                                                                                                                                        gAGTGAACTCATAGCAGCGTGCGGTGATTTCTTCCGGCGTCAGCCAGCAAACTTCACTGACTTCATCTTCCTGTAGTGCGAAGGGACCGTGAGAGACGCAGCTGAACAATGCGCCCCAGACACGGCAATTTTTATCTTCGAAATAGAACt             <  1:3978165/150‑1 (MQ=255)

                                                                                                                                            gAACTCATCGCAGCGTGCGGTGATTTCTTCCGGCGTCAGCCAGCAAACTTCACTGACTTCATCTTCCTGTAGTGCGAAGGGACCGTGAGAGACGCAGCTGAACAATGCGCCCCAGACACGGCAATTTTTATCTTCGAAATAGAACTGccc         >  2:27557/1‑150 (MQ=255)

                                                                                                                                            gAACTCATCGCAGCGTGCGGTGATTTCTTCCGGCGTCAGCCAGCAAACTTCACTGACTTCATCTTCCTGTAGTGCGAAGGGACCGTGAGAGACGCAGCTGAACAATGCGCCCCAGACACGGCAATTTTTATCTTCGAAATAGAACTGccc         <  2:1795756/150‑1 (MQ=255)

                                                                                                                                                 cATCGCAGCGTGCGGTGATTTCTTCCGGCGTCAGCCAGCAAACTTCACTGACTTCATCTTCCTGTAGTGCGAAGGGACCGTGAGAGACGCAGCTGAACAATGCGCCCCAGACACGGCAATTTTTATCTTCGAAATAGAACTGCCCGTGCt    <  2:4662754/150‑1 (MQ=255)

                                                                                                                                                 cATAGCAGCGTGCGGTGATTTCTTCCGGCGTCAGCCAGCAAACTTCACTGACTTCATCTTCCTGTAGTGCGAAGGGACCGTGAGAGACGCAGCTGAACAATGCGCCCCAGACACGGCAATTTTTATCTTCGAAATAGAACTGCCCGTGCt    <  2:2053536/150‑1 (MQ=255)

GCTCAGTTGATGGGCTACCACTAAAAATGTTAAGAGCCGCAATTGCGGCTCTTTTTTCATTCTGCCGTTTCAGTCTCTACGGCTTCATTTTTGGCATTGCGCTTCATCCACAACGCTAGCGCTTTCAGCGAGTCTGGAGTGAACTCATCGCAGCGTGCGGTGATTTCTTCCGGCGTCAGCCAGCAAACTTCACTGACTTCATCTTCCTGTAGTGCGAAGGGACCGTGAGAGACGCAGCTGAACAATGCGCCCCAGACACGGCAATTTTTATCTTCGAAATAGAACTGCCCGTGCTCG  >  CP009273/2412057‑2412353

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`