Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,540,445 G→T 5.5% A138A (GCG→GCT)  murP → N‑acetylmuramic acid permease, EIIBC component, PTS system

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,540,4450GT 5.5% 1302.1 / 32.8 324A138A (GCG→GCT) murPN‑acetylmuramic acid permease, EIIBC component, PTS system
Reads supporting (aligned to +/- strand):  ref base G (156/150);  new base T (8/10);  total (164/160)
Fisher's exact test for biased strand distribution p-value = 6.35e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01

Only 100 of 327 total aligned reads displayed.

CAGGATGCCGCAGAAATCGCCGCCCAGAACAAACGTCAGTTAAAAGCCAAACAAACCTCCGGCGTGCAACAATTTCTCGCCAAATTCGCCACCATCTTCACGCCGCTGATCCCCGGTTTTATTGCCGCCGGTCTGCTGCTGGGGATAGCGACGTTAATTGCCACGGTGATGCACGTTCCGGCAGATGCTCAGGGAACACTACCCGATGCGCTGAATTTTATGAAGGTGTTCAGCAAAGGTTTGTTCACTTTCCTGGTGATTCTGGTGGGCTATAACGCCGCCCAGGCATTTGGCGG  >  CP009273/2540296‑2540591
                                                                                                                                                     |                                                                                                                                                  
 aGGATGCCGCAGAAATCGCCGCCCAGAACAAACGTCAGTTAAAAGCCAAACAAACCTCCGGCGTGCAACAATTTCTCGCCAAATTCGCCACCATCTTCACGCCGCTGATCCCCGGTTTTATTGCCGCCGGTCTGCTGCTGGGGATAGCGa                                                                                                                                                   >  1:714748/1‑150 (MQ=255)
  ggATGCCGCAGAAATCGCCGCCCAGAACAAACGTCAGTTAAAAGCCAAACAAACCTCCGGCGTGCAACAATTTCTCGCCAAATTCGCCACCATCTTCACGCCGCTGATCCCCGGTTTTATTGCCGCCGGTCTGCTGCTGGGGATAGCGAc                                                                                                                                                  <  1:4458774/150‑1 (MQ=255)
   gATGCCGCAGAAATCGCCGCCCAGAACAAACGTCAGTTAAAAGCCAAACAAACCTCCGGCGTGCAACAATTTCTCGCCAAATTCGCCACCATCTTCACGCCGCTGATCCCCGGTTTTATTGCCGCCGGTCTGCTGCTGGGGATAGCGACg                                                                                                                                                 <  2:569930/150‑1 (MQ=255)
   gATGCCGCAGAAATCGCCGCCCAGAACAAACGTCAGTTAAAAGCCAAACAAACCTCCGGCGTGCAACAATTTCTCGCCAAATTCGCCACCATCTTCACGCCGCTGATCCCCGGTTTTATTGCCGCCGGTCTGCTGCTGGGGATAGCGACg                                                                                                                                                 <  1:1244539/150‑1 (MQ=255)
   gATGCCGCAGAAATCGCCGCCCAGAACAAACGTCAGTTAAAAGCCAAACAAACCTCCGGCGTGCAACAATTTCTCGCCAAATTCGCCACCATCTTCACGCCGCTGATCCCCGGTTTTATTGCCGCCGGTCTGCTGCTGGGGATAGCGACg                                                                                                                                                 <  2:2565073/150‑1 (MQ=255)
    aTGCCGCAGAAATCGCCGCCCAGAACAAACGTCAGTTAAAAGCCAAACAAACCTCCGGCGTGCAACAATTTCTCGCCAAATTCGCCACCATCTTCACGCCGCTGATCCCCGGTTTTATTGCCGCCGGTCTGCTGCTGGGGATAGCGACGt                                                                                                                                                >  2:2206484/1‑150 (MQ=255)
      gCCGCAGAAATCGCCGCCCAGAACAAACGTCAGTTAAAAGCCAAACAAACCTCCGGCGTGCAACAATTTCTCGCCAAATTCGCCACCATCTTCACGCCGCTGATCCCCGGTTTTATTGCCGCCGGTCTGCTGCTGGGGATAGCGACGTTa                                                                                                                                              >  1:2485103/1‑150 (MQ=255)
       ccGCAGAAATCGCCGCCCAGAACAAACGTCAGTTAAAAGCCAAACAAACCTCCGGCGTGCAACAATTTCTCGCCAAATTCGCCACCATCTTCACGCCGCTGATCCCCGGTTTTATTGCCGCCGGTCTGCTGCTGGGGATAGCGACGTTaa                                                                                                                                             >  1:951876/1‑150 (MQ=255)
       ccGCAGAAATCGCCGCCCAGAACAAACGTCAGTTAAAAGCCAAACAAACCTCCGGCGTGCAACAATTTCTCGCCAAATTCGCCACCATCTTCACGCCGCTGATCCCCGGTTTTATTGCCGCCGGTCTGCTGCTGGGGATAGCGACGTTaa                                                                                                                                             <  1:4581340/150‑1 (MQ=255)
        cGCAGAAATCGCCGCCCAGAACAAACGTCAGTTAAAAGCCAAACAAACCTCCGGCGTGCAACAATTTCTCGCCAAATTCGCCACCATCTTCACGCCGCTGATCCCCGGTTTTATTGCCGCCGGTCTGCTGCTGGGGATAGCGACGATAAt                                                                                                                                            >  1:2704214/1‑150 (MQ=255)
           aGAAATCGCCGCCCAGAACAAACGTCAGTTAAAAGCCAAACAAACCTCCGGCGTGCAACAATTTCTCGCCAAATTCGCCACCATCTTCACGCCGCTGATCCCCGGTTTTATTGCCGCCGGTCTGCTGCTGGGGATAGCGACGTTAATTGc                                                                                                                                         <  1:4981470/150‑1 (MQ=255)
            gAAATCGCCGCCCAGAACAAACGTCAGTTAAAAGCCAAACAAACCTCCGGCGTGCAACAATTTCTCGCCAAATTCGCCACCATCTTCACGCCGCTGATCCCCGGTTTTATTGCCGCCGGTCTGCTGCTGGGGATAGCGACGTTAATTGcc                                                                                                                                        <  1:2415267/150‑1 (MQ=255)
             aaaTCGCCGCCCAGAACAAACGTCAGTTAAAAGCCAAACAAACCTCCGGCGTGCAACAATTTCTCGCCAAATTCGCCACCATCTTCACGCCGCTGATCCCCGGTTTTATTGCCGCCGGTCTGCTGCTGGGGATAGCGACGTTAATTGCCa                                                                                                                                       <  1:4711634/150‑1 (MQ=255)
              aaTCGCCGCCCAGAACAAACGTCAGTTAAAAGCCAAACAAACCTCCGGCGTGCAACAATTTCTCGCCAAATTCGCCACCATCTTCACGCCGCTGATCCCCGGTTTTATTGCCGCCGGTCTGCTGCTGGGGATAGCGACGTTAATTGCCAc                                                                                                                                      <  1:4236444/150‑1 (MQ=255)
              aaTCGCCGCCCAGAACAAACGTCAGTTAAAAGCCAAACAAACCTCCGGCGTGCAACAATTTCTCGCCAAATTCGCCACCATCTTCACGCCGCTGATCCCCGGTTTTATTGCCGCCGGTCTGCTGCTGGGGATAGCGACGTTAATTGCCAc                                                                                                                                      <  1:915796/150‑1 (MQ=255)
               aTCGCCGCCCAGAACAACCGTCAGTTAAAAGCCAAACAAACCTCCGGCGTGCAACAATTTCTCGCCAAATTCGCCACCATCTTCACGCCGCTGATCCCCGGTTTTATTGCCGCCGGTCTGCTGCTGGGGATAGCGACGTTAATTGCCACg                                                                                                                                     <  2:3655198/150‑1 (MQ=255)
               aTCGCCGCCCAGAACAAACGTCAGTTAAAAGCCAAACAAACCTCCGGCGTGCAACAATTTCTCGCCAAATTCGCCACCATCTTCACGCCGCTGATCCCCGGTTTTATTGCCGCCGGTCTGCTGCTGGGGATAGCGACGTTAATTGCCACg                                                                                                                                     <  1:1448806/150‑1 (MQ=255)
                  gccgccCAGAACAAACGTCAGTTAAAAGCCAAACAAACCTCCGGCGTGCAACAATTTCTCGCCAAATTCGCCACCATCTTCACGCCGCTCATCCCCGGTTTTATTGCCGCCGGTCTGCTGCTGGGGATAGCGACGTTAATTGCCACGGTg                                                                                                                                  >  2:1840317/1‑150 (MQ=255)
                    cgccCAGAACAAACGTCAGTTAAAAGCCAAACAAACCTCCGGCGTGCAACAATTTCTCGCCAAATTCGCCACCATCTTCACGCCGCTGATCCCCGGTTTTATTGCCGCCGGTCTGCTGCTGGGGATAGCGACGTTAATTGCCACGGTGAt                                                                                                                                <  1:1886352/150‑1 (MQ=255)
                     gccCAGAACAAACGTCAGTTAAAAGCCAAACAAACCTCCGGCGTGCAACAATTTCTCGCCAAATTCGCCACCATCTTCACGCCGCTGATCCCCGGTTTTATTGCCGCCGGTCTGCTGCTGGGGATAGCGACGTTAATTGCCACGGTGATg                                                                                                                               <  2:4537537/150‑1 (MQ=255)
                      cccAGAACAAACGTCAGTTAAAAGCCAAACAAACCTCCGGCGTGCAACAATTTCTCGCCAAATTCGCCACCATCTTCACGCCGCTGATCCCCGGTTTTATTGCCGCCGGTCTGCTGCTGGGGATAGCTACGTTAATTGCCACGGTGATGc                                                                                                                              <  2:2329433/150‑1 (MQ=255)
                         aGAACAAACGTCAGTTAAAAGCCAAACAAACCTCCGGCGTGCAACAATTTCTCGCCAAATTCGCCACCATCTTCACGCCGCTGATCCCCGGTTTTATTGCCGCCGGTCTGCTGCTGGGGATAGCGACGTTAATTGCCACGGTGATGCACg                                                                                                                           >  2:1533202/1‑150 (MQ=255)
                         aGAACAAACGTCAGTTAAAAGCCAAACAAACCTCCGGCGTGCAACAATTTCTCGCCAAATTCGCCACCATCTTCACGCCGCTGATCCCCGGGTTTATTGCCGCCGGTCTGCTGCTGGGGATAGCGACGTTAATTGCCACGGTGATGCACg                                                                                                                           >  2:4949725/1‑150 (MQ=255)
                          gAACAAACGTCAGTTAAAAGCCAAACAAACCTCCGGCGTGCAACAATTTCTCGCCAAATTCGCCACCATCTTCACGCCGCTGATCCCCGGTTTTATTGCCGCCGGTCTGCTGCTGGGGATAGCGACGTTAATTGCCACGGTGATGCACGt                                                                                                                          >  2:1066557/1‑150 (MQ=255)
                          gAACAAACGTCAGTTAAAAGCCAAACAAACCTCCGGCGTGCAACAATTTCTCGCCAAATTCGCCACCATCTTCACGCCGCTGATCCCCGGTTTTATTGCCGCCGGTCTGCTGCTGGGGATAGCGACGTTAATTGCCACGGTGATGCACGt                                                                                                                          >  1:5123227/1‑150 (MQ=255)
                               aaCGTCAGTTAAAAGCCAAACAAACCTCCGGCGTGCAACAATTTCTCGCCAAATTCGCCACCATCTTCACGCCGCTGATCCCCGGTTTTATTGCCGCCGGTCTGCTGCTGGGGATAGCGACGTTAATTGCCACGGTGATGCACGTTCCgg                                                                                                                     >  2:4277745/1‑150 (MQ=255)
                                 cGTCAGTTAAAAGCCAAACAAACCTCCGGCGTGCAACAATTTCTCGCCAAATTCGCCACCATCTTCACGCCGCTGATCCCCGGTTTTATTGCCGCCGGTCTGCTGCTGGGGATAGCGACGTTAATTGCCACGGTGATGCACGTTCCGGCa                                                                                                                   <  1:4140401/150‑1 (MQ=255)
                                 cGTCAGTTAAAAGCCAAACAAACCTCCGGCGTGCAACAATTTCTCGCCAAATTCGCCACCATCTTCACGCCGCTGATCCCCGGTTTTATTGCCGCCGGTCTGCTGCTGGGGATAGCGACGTTAATTGCCACGGTGATGCACGTTCCGGCa                                                                                                                   >  1:3100562/1‑150 (MQ=255)
                                 cGTCAGTTAAAAGCCAAACAAACCTCCGGCGTGCAACAATTTCTCGCCAAATTCGCCACCATCTTCACGCCGCTGATCCCCGGTTTTATTGCCGCCGGTCTGCTGCTGGGGATAGCGACGTTAATTGCCACGGTGATGCACGTTCCGGCa                                                                                                                   <  1:1377377/150‑1 (MQ=255)
                                    cAGTTAAAAGCCAAACAAACCTCCGGCGTGCAACAATTTCTCGCCAAATTCGCCACCATCTTCACGCCGCTGATCCCCGGTTTTATTGCCGCCGGTCTGCTGCTGGGGATAGCGACGTTAATTGCCACGGTGATGCACGTTCCGGCAGAt                                                                                                                >  2:2077816/1‑150 (MQ=255)
                                    cAGTTAAAAGCCAAACAAACCTCCGGCGTGCAACAATTTCTCGCCAAATTCGCCACCATCTTCACGCCGCTGATCCCCGGTTTTATTGCCGCCGGTCTGCTGCTGGGGATAGCGACGTTAATTGCCACGGTGATGCACGTTCCGGCAGAt                                                                                                                >  2:4227967/1‑150 (MQ=255)
                                    cAGTTAAAAGCCAAACAAACCTCCGGCGTGCAACAATTTCTCGCCAAATTCGCCACCATCTTCACGCCGCTGATCCCCGGTTTTATTGCCGCCGGTCTGCTGCTGGGGATAGCGACGTTAATTGCCACGGTGATGCACGTTCCGGCAGAt                                                                                                                >  1:1121489/1‑150 (MQ=255)
                                     aGTTAAAAGCCAAACAAACCTCCGGCGTGCAACAATTTCTCGCCAAATTCGCCACCATCTTCACGCCGCTGATCCCCGGTTTTATTGCCGCCGGTCTGCTGCTGGGGATAGCGACGTTAATTGCCACGGTGATGCACGTTCCGGCAGATg                                                                                                               >  1:604632/1‑150 (MQ=255)
                                     aGTTAAAAGCCAAACAAACCTCCGGCGTGCAACAATTTCTCGCCAAATTCGCCACCATCTTCACGCCGCTGATCCCCGGTTTTATTGCCGCCGGTCTGCTGCTGGGGATAGCGACGTTAATTGCCACGGTGATGCACGTTCCGGCAGATg                                                                                                               <  2:4098409/150‑1 (MQ=255)
                                        tAAAAGCCAAACAAACCTCCGGCGTGCAACAATTTCTCGCCAAATTCGCCACCATCTTCACGCCGCTGATCCCCGGTTTTATTGCCGCCGGTCTGCTGCTGGGGATAGCGACGTTAATTGCCACGGTGATGCACGTTCCGGCAGATGCTc                                                                                                            <  1:2608377/150‑1 (MQ=255)
                                         aaaaGCCAAACAAACCTCCGGCGTGCAACAATTTCTCGCCAAATTCGCCACCATCTTCACGCCGCTGATCCCCGGTTTTATTGCCGCCGGTCTGCTGCTGGGGATAGCGACGTTAATTGCCACGGTGATGCACGTTCCGGCAGATGCTCa                                                                                                           >  2:3863899/1‑150 (MQ=255)
                                             gCCAAACAAACCTCCGGCGTGCAACAATTTCTCGCCAAATTCGCCACCATCTTCACGCCGCTGATCCCCGGTTTTATTGCCGCCGGTCTGCTGCTGGGGATAGCGACGTTAATTGCCACGGTGATGCACGTTCCGGCAGATGCTCAGGGa                                                                                                       <  2:5063103/150‑1 (MQ=255)
                                               caaacaaaCCTCCGGCGTGCAACAATTTCTCGCCAAATTCGCCACCATCTTCACGCCGCTGATCCCCGGTTTTATTGCCGCCGGTCTGCTGCTGGGGATAGCGACGTTAATTGCCACGGTGATGCACGTTCCGGCAGATGCTCAGGGAac                                                                                                     >  2:248014/1‑150 (MQ=255)
                                               caaacaaaCCTCCGGCGTGCAACAATTTCTCGCCAAATTCGCCACCATCTTCACGCCGCTGATCCCCGGTTTTATTGCCGCCGGTCTGCTGCTGGGGATAGCGACGTTAATTGCCACGGTGATGCACGTTCCGGCAGATGCTCAGGGAac                                                                                                     >  2:3211035/1‑150 (MQ=255)
                                               caaacaaaCCTCCGGCGTGCAACAATTTCTCGCCAAATTCGCCACCATCTTCACGCCGCTGATCCCCGGTTTTATTGCCGCCGGTCTGCTGCTGGGGATAGCGACGTTAATTGCCACGGTGATGCACGTTCCGGCAGATGCTCAGGGAac                                                                                                     >  1:4929813/1‑150 (MQ=255)
                                                  acaaacCTCCGGCGTGCAACAATTTCTCGCCAAATTCGCCACCATCTTCACGCCGCTGATCCCCGGTTTTATTGCCGCCGGTCTGCTGCTGGGGATAGCGACGTTAATTGCCACGGTGATGCACGTTCCGGCAGATGCTCAGGGAACACt                                                                                                  >  2:1943094/1‑150 (MQ=255)
                                                  acaaacCTCCGGCGTGCAACAATTTCTCGCCAAATTCGCCACCATCTACACGCCGCTGATCCCCGGTTTTATTGCCGCCGGTCTGCTGCTGGGGATAGCGACGTTAATTGCCACGGTGATGCACGTTCCGGCAGATGCTCAGGGAACACt                                                                                                  <  2:1827168/150‑1 (MQ=255)
                                                     aacCTCCGGCGTGCAACAATTTCTCGCCAAATTCGCCACCATCTTCACGCCGCTGATCCCCGGTTTTATTGCCGCCGGTCTGCTGCTGGGGATAGCGACGTTAATTGCCACGGTGATGCACGTTCCGGCAGATGCTCAGGGAACACTAcc                                                                                               >  1:2043455/1‑150 (MQ=255)
                                                       ccTCCGGCGTGCAACAATTTCTCGCCAAATTCGCCACCATCTTCACGCCGCTGATCCCCGGTTTTATTGCCGCCGGTCTGCTGCTGGGGATAGCGACGTTAATTGCCACGGTGATGCACGTTCCGGCAGATGCTCAGGGAACACTACCCg                                                                                             >  2:2963722/1‑150 (MQ=255)
                                                          ccGGCGTGCAACAATTTCTCGCCAAATTCGCCACCATCTTCACGCCGCTGATCCCCGGTTTTATTGCCGCCGGTCTGCTGCTGGGGATAGCGACGTTAATTGCCACGGTGATGCACGTTCCGGCAGATGCTCAGGGAACACTACCCGATg                                                                                          >  2:884683/1‑150 (MQ=255)
                                                          ccGGCGTGCAACAATTTCTCGCCAAATTCGCCACCATCTTCACGCCGCTGATCCCCGGTTTTATTGCCGCCGGTCTGCTGCTGGGGATAGCGACGTTAATTGCCACGGTGATGCACGTTCCGGCAGATGCTCAGGGAACACTACCCGATg                                                                                          >  1:3051672/1‑150 (MQ=255)
                                                           cGGCGTGCAACAATTTCTCGCCAAATTCGCCACCATCTTCACGCCGCTGATCCCCGGTTTTATTGCCGCCGGTCTGCTGCTGGGGATAGCGACGTTAATTGCCACGGTGATGCACGTTCCGGCAGATGCTCAGGGAACACTACCCGATgc                                                                                         <  2:1427725/150‑1 (MQ=255)
                                                           cGGCGTGCAACAATTTCTCGCCAAATTCGCCACCATCTTCACGCCGCTGATCCCCGGTTTTATTGCCGCCGGTCTGCTGCTGGGGATAGCGACGTTAATTGCCACGGTGATGCACGTTCCGGCAGATGCTCAGGGAACACTACCCGATgc                                                                                         >  1:2742492/1‑150 (MQ=255)
                                                            ggCGTGCAACAATTTCTCGCCAAATTCGCCACCATCTTCACGCCGCTGATCCCCGGTTTTATTGCCGCCGGTCTGCTGCTGGGGATAGCGACGTTAATTGCCACGGTGATGCACGTTCCGGCAGATGCTCAGGGAACACTACCCGATgcg                                                                                        >  2:1250563/1‑150 (MQ=255)
                                                              cGTGCAACAATTTCTCGCCAAATTCGCCACCATCTTCACGCCGCTGATCCCCGGTTTTATTGCCGCCGGTCTGCTGCTGGGGATAGCGACGTTAATTGCCACGGTGATGCACGTTCCGGCAGATGCTCAGGGAACACTACCCGATGCGCt                                                                                      >  1:2735097/1‑150 (MQ=255)
                                                                tGCAACAATTTCTCGCCAAATTCGCCACCATCTTCACGCCGCTGATCCCCGGTTTTATTGCCGCCGGTCTGCTGCTGGGGATAGCGACGTTAATTGCCACGGTGATGCACGTTCCGGCAGATGCTCAGGGAACACTACCCGATGCGCTGa                                                                                    <  1:581361/150‑1 (MQ=255)
                                                                    acaaTTTCTCGCCAAATTCGCCACCATCTTCACGCCGCTGATCCCCGGTTTTATTGCCGCCGGTCTGCTGCTGGGGATAGCGACGTTAATTGCCACGGTGATGCACGTTCCGGCAGATGCTCAGGGAACACTACCCGATGCGCTGAAttt                                                                                <  1:3661586/150‑1 (MQ=255)
                                                                      aaTTTCTCGCCAAATTCGCCACCATCTTCACGCCGCTGATCCCCGGTTTTATTGCCGCCGGTCTGCTGCTGGGGATAGCGACGTTAATTGCCACGGTGATGCACGTTCCGGCAGATGCTCAGGGAACACTACCCGATGCGCTGAATTTTa                                                                              <  1:97163/150‑1 (MQ=255)
                                                                      aaTTTCTCGCCAAATTCGCCACCATCTTCACGCCGCTGATCCCCGGTTTTATTGCCGCCGGTCTGCTGCTGGGGATAGCGACGTTAATTGCCACGGTGATGCACGTTCCGGCAGATGCTCAGGGAACACTACCCGATGCGCTGAATTTTa                                                                              >  1:284571/1‑150 (MQ=255)
                                                                      aaTTTCTCGCCAAATTCGCCACCATCTTCACGCCGCTGATCCCCGGTTTTATTGCCGCCGGTCTGCTGCTGGGGATAGCGACGTTAATTGCCACGGTGATGCACGTTCCGGCAGATGCTCAGGGAACACTACCCGATGCGCTGAATTTTa                                                                              >  1:500779/1‑150 (MQ=255)
                                                                               ccAAATTCGCCACCATCTTCACGCCGCTGATCCCCGGTTTTATTGCCGCCGGTCTGCTGCTGGGGATAGCGACGTTAATTGCCACGGTGATGCACGTTCCGGCAGATGCTCAGGGAACACTACCCGATGCGCTGAATTTTATGAAGgtgt                                                                     >  2:439315/1‑150 (MQ=255)
                                                                               ccAAATTCGCCACCATCTTCACGCCGCTGATCCCCGGTTTTATTGCCGCCGGTCTGCTGCTGGGGATAGCGACGTTAATTGCCACGGTGATGCACGTTCCGGCAGATGCTCAGGGAACACTACCCGATGCGCTGAATTTTATGAAGgtgt                                                                     <  1:1840202/150‑1 (MQ=255)
                                                                                cAAATTCGCCACCATCTTCACGCCGCTGATCCCCGGTTTTATTGCCGCCGGTCTGCTGCTGGGGATAGCGACGTTAATTGCCACGGTGATGCACGTTCCGGCAGATGCTCAGGGAACACTACCCGATGCGCTGAATTTTATGAAGGTGtt                                                                    <  2:3445308/150‑1 (MQ=255)
                                                                                cAAATTCGCCACCATCTTCACGCCGCTGATCCCCGGTTTTATTGCCGCCGGTCTGCTGCTGGGGATAGCGACGTTAATTGCCACGGTGATGCACGTTCCGGCAGATGCTCAGGGAACACTACCCGATGCGCTGAATTTTATGAAGGTGtt                                                                    <  2:940787/150‑1 (MQ=255)
                                                                                  aagtCGCCACCATCTTCACGCCGCTGATCCCCGGTTTTATTGCCGCCGGTCTGCTGCTGGGGATAGCGACGTTAATTGCCACGGTGATGCACGTTCCGGCAGATGCTCAGGGAACACTACCCGATGCGCTGAATTTTATGAAGGTGTTCa                                                                  <  2:2904394/147‑1 (MQ=255)
                                                                                  aaTTCGCCACCATCTTCACGCCGCTGATCCCCGGTTTTATTGCCGCCGGTCTGCTGCTGGGGATAGCGACGTTAATTGCCACGGTGATGCACGTTCCGGCAGATGCTCAGGGAACACTACCCGATGCGCTGAATTTTATGAAGGTGTTCa                                                                  >  2:1402372/1‑150 (MQ=255)
                                                                                   aTTCGCCACCATCTTCACGCCGCTGATCCCCGGTTTTATTGCCGCCGGTCTGCTGCTGGGGATAGCGACGTTAATTGCCACGGTGATGCACGTTCCGGCAGATGCTCAGGGAACACTACCCGATGCGCTGAATTTTATGAAGGTGTTCAg                                                                 <  1:4601313/150‑1 (MQ=255)
                                                                                      cGCCACCATCTTCACGCCGCTGATCCCCGGTTTTATTGCCGCCGGTCTGCTGCTGGGGATAGCGACGTTAATTGCCACGGTGATGCACGTTCCGGCAGATGCTCAGGGAACACTACCCGATGCGCTGAATTTTATGAAGGTGTTCAGCaa                                                              <  1:391640/150‑1 (MQ=255)
                                                                                       gCCACCATCTTCACGCCGCTGATCCCCGGTTTTATTGCCGCCGGTCTGCTGCTGGGGATAGCGACGTTAATTGCCACGGTGATGCACGTTCCGGCAGATGCTCAGGGAACACTACCCGATGCGCTGAATTTTATGAAGGTGTTCAGCaaa                                                             >  2:3587423/1‑150 (MQ=255)
                                                                                       gCCACCATCTTCACGCCGCTGATCCCCGGTTTTATTGCCGCCGGTCTGCTGCTGGGGATAGCGACGTTAATTGCCACGGTGATGCACGTTCCGGCAGATGCTCAGGGAACACTACCCGATGCGCTGAATTTTATGAAGGTGTTCAGCaaa                                                             >  1:3147790/1‑150 (MQ=255)
                                                                                       gCCACCATCTTCACGCCGCTGATCACCGGTTTTATTGCCGCCGGTCTGCTGCTGGGGATAGCGACGTTAATTGCCACGGTGATGCACGTTCCGGCAGATGCTCAGGGAACACTACCCGATGCGCTGAATTTTATGAAGGTGTTCAGCaaa                                                             >  2:2685364/1‑150 (MQ=255)
                                                                                        ccaccaTCTTCACGCCGCTGATCCCCGGTTTTATTGCCGCCGGTCTGCTGCTGGGGATAGCTACGTTAATTGCCACGGTGATGCACGTTCCGGCAGATGCTCAGGGAACACTACCCGATGCGCTGAATTTTATGAAGGTGTTCAGCAAAg                                                            <  1:3273781/150‑1 (MQ=255)
                                                                                        ccaccaTCTTCACGCCGCTGATCCCCGGTTTTATTGCCGCCGGTCTGCTGCTGGGGATAGCGACGTTAATTGCCACGGTGATGCACGTTCCGGCAGATGCTCAGGGAACACTACCCGATGCGCTGAATTTTATGAAGGTGTTCAGCAAAg                                                            <  2:4269673/150‑1 (MQ=255)
                                                                                        ccaccaTCTTCACGCCGCTGATCCCCGGTTTTATTGCCGCCGGTCTGCTGCTGGGGATAGCGACGTTAATTGCCACGGTGATGCACGTTCCGGCAGATGCTCAGGGAACACTACCCGATGCGCTGAATTTTATGAAGGTGTTCAGCAAAg                                                            <  2:721326/150‑1 (MQ=255)
                                                                                         caccaTCTTCACGCCGCTGATCCCCGGTTTTATTGCCGCCGGTCTGCTGCTGGGGATAGCGACGTTAATTGCCACGGTGATGCACGTTCCGGCAGATGCTCAGGGAACACTACCCGATGCGCTGAATTTTATGAAGGTGTTCAGCAAAgg                                                           >  1:2662459/1‑150 (MQ=255)
                                                                                         caccaTCTTCACGCCGCTGATCCCCGGTTTTATTGCCGCCGGTCTGCTGCTGGGGATAGCGACGTTAATTGCCACGGTGATGCACGTTCCGGCAGATGCTCAGGGAACACTACCCGATGCGCTGAATTTTATGAAGGTGTTCAGCAAAgg                                                           <  1:1194976/150‑1 (MQ=255)
                                                                                         caccaTCGTCACGCCGCTGATCCCCGGTTTTATTGCCGCCGGTCTGCTGCTGGGGATAGCTACGTTAATTGCCACGGTGATGCACGTTCCGGCAGATGCTCAGGGAACACTACCCGATGCGCTGAATTTTATGAAGGTGTTCAGCAAAgg                                                           <  1:4051500/150‑1 (MQ=255)
                                                                                          accaTCTTCACGCCGCTGATCCCCGGTTTTATTGCCGCCGGTCTGCTGCTGGGGATAGCGACGTTAATTGCCACGGTGATGCACGTTCCGGCAGATGCTCAGGGAACACTACCCGATGCGCTGAATTTTATGAAGGTGTTCAGCAAAGGt                                                          <  1:1589381/150‑1 (MQ=255)
                                                                                             aTCTTCACGCCGCTGATCCCCGGTTTTATTGCCGCCGGTCTGCTGCTGGGGATAGCTACGTTAATTGCCACGGTGATGCACGTTCCGGCAGATGCTCAGGGAACACTACCCGATGCGCTGAATTTTATGAAGGTGTTCAGCAAAGGTTTg                                                       >  2:461180/1‑150 (MQ=255)
                                                                                              tCTTCACGCCGCTGATCCCCGGTTTTATTGCCGCCGGTCTGCTGCTGGGGATAGCGACGTTAATTGCCACGGTGATGCACGTTCCGGCAGATGCTCAGGGAACACTACCCGATGCGCTGAATTTTATGAAGGTGTTCAGCAAAGGTTTGt                                                      <  1:2572268/150‑1 (MQ=255)
                                                                                                    cgccgcTGATCCCCGGTTTTATTGCCGCCGGTCTGCTGCTGGGGATAGCGACGTTAATTGCCACGGTGATGCACGTTCCGGCAGATGCTCAGGGAACACTACCCGATGCGCTGAATTTTATGAAGGTGTTCAGCAAAGGTTTGTTCACtt                                                >  2:109377/1‑150 (MQ=255)
                                                                                                    cgccgcTGATCCCCGGTTTTATTGCCGCCGGTCTGCTGCTGGGGATAGCGACGTTAATTGCCACGGTGATGCACGTTCCGGCAGATGCTCAGGGAACACTACCCGATGCGCTGAATTTTATGAAGGTGTTCAGCAAAGGTTTGTTCACtt                                                <  2:4788191/150‑1 (MQ=255)
                                                                                                    cgccgcTGAGCCCCGGTTTTAGTGCCGCCGGTCTGCTGCTGGGGATAGCGACGTTAATTGCCACGGTGATGCACGTTCCGGCAGATGCTCAGGGAACACTACCCGATGCGCTGAATTTTATGAAGGTGTTCAGCAAAGGTTTGTTCACtt                                                <  1:3694977/150‑1 (MQ=255)
                                                                                                     gccgcTGATCCCCGGTTTTATTGCCGCCGGTCTGCTGCTGGGGATAGCGACGTTAATTGCCACGGTGATGCACGTTCCGGCAGATGCTCAGGGAACACTACCCGATGCGCTGAATTTTATGAAGGTGTTCAGCAAAGGTTTGTTCACttt                                               >  1:2116164/1‑150 (MQ=255)
                                                                                                       cgcTGATCCCCGGTTTTATTGCCGCCGGTCTGCTGCTGGGGATAGCGACGTTAATTGCCACGGTGATGCACGTTCCGGCAGATGCTCAGGGAACACTACCCGATGCGCTGAATTTTATGAAGGTGTTCAGCAAAGGTTTGTTCACTTTcc                                             <  2:1353992/150‑1 (MQ=255)
                                                                                                          tGATCCCCGGTTTTATTGCCGCCGGTCTGCTGCTGGGGATAGCGACGTTAATTGCCACGGTGATGCACGTTCCGGCAGATGCTCAGGGAACACTACCCGATGCGCTGAATTTTATGAAGGTGTTCAGCAAAGGTTTGTTCACTTTCCTgg                                          <  2:4854009/150‑1 (MQ=255)
                                                                                                                ccGGTTTTATTGCCGCCGGTCTGCTGCTGGGGATAGCGACGTTAATTGCCACGGTGATGCACGTTCCGGCAGATGCTCAGGGAACACTACCCGATGCGCTGAATTTTATGAAGGTGTTCAGCAAAGGTTTGTTCACTTTCCTGGTGATTc                                    >  2:4188009/1‑150 (MQ=255)
                                                                                                                   gTTTTATTGCCGCCGGTCTGCTGCTGGGGATAGCGACGTTAATTGCCACGGTGATGCACGTTCCGGCAGATGCTCAGGGAACACTACCCGATGCGCTGAATTTTATGAAGGTGTTCAGCAAAGGTTTGTTCACTTTCCTGGTGATTCtgg                                 >  2:5021295/1‑150 (MQ=255)
                                                                                                                   gTTTTATTGCCGCCGGTCTGCTGCTGGGGATAGCGACGTTAATTGCCACGGTGATGCACGTTCCGGCAGATGCTCAGGGAACACTACCCGATGCGCTGAATTTTATGAAGGTGTTCAGCAAAGGTTTGTTCACTTTCCTGGTGATTCtgg                                 <  1:1642309/150‑1 (MQ=255)
                                                                                                                        aTTGCCGCCGGTCTGCTGCTGGGGATAGCGACGTTAATTGCCACGGTGATGCACGTTCCGGCAGATGCTCAGGGAACACTACCCGATGCGCTGAATTTTATGAAGGTGTTCAGCAAAGGTTTGTTCACTTTCCTGGTGATTCTGGTGGGc                            >  2:2058436/1‑150 (MQ=255)
                                                                                                                         ttGCCGCCGGTCTGCTGCTGGGGATAGCGACGTTAATTGCCACGGTGATGCACGTTCCGGCAGATGCTCAGGGAACACTACCCGATGCGCTGAATTTTATGAAGGTGTTCAGCAAAGGTTTGTTCACTTTCCTGGTGATTCTGGTGGGCt                           >  2:1765108/1‑150 (MQ=255)
                                                                                                                          tGCCGCCGGTCTGCTGCTGGGGATAGCTACGTTAATTGCCACGGTGATGCACGTTCCGGCAGATGCTCAGGGAACACTACCCGATGCGCTGAATTTTATGAAGGTGTTCAGCAAAGGTTTGTTCACTTTCCTGGTGATTCTGGTGGGCta                          >  1:2854941/1‑150 (MQ=255)
                                                                                                                          tGCCGCCGGTCTGCTGCTGGGGATAGCGACGTTAATTGCCACGGTGATGCACGTTCCGGCAGATGCTCAGGGAACACTACCCGATGCGCTGAATTTTATGAAGGTGTTCAGCAAAGGTTTGTTCACTTTCCTGGTGATTCTGGTGGGCta                          >  2:4498983/1‑150 (MQ=255)
                                                                                                                               ccgGTCTGCTGCTGGGGATAGCGACGTTAATTGCCACGGTGATGCACGTTCCGGCAGATGCTCAGGGAACACTACCCGATGCGCTGAATTTTATGAAGGTGTTCAGCAAAGGTTTGTTCACTTTCCTGGTGATTCTGGTGGGCTATAAcg                     <  1:1009309/150‑1 (MQ=255)
                                                                                                                                     tgctgctgGGGATAGCGACGTTAATTGCCACGGTGATGCACGTTCCGGCAGATGCTCAGGGAACACTACCCGATGCGCTGAATTTTATGAAGGTGTTCAGCAAAGGTTTGTTCACTTTCCTGGTGATTCTGGTGGGCTATAACGCCGccc               <  1:4380832/150‑1 (MQ=255)
                                                                                                                                      gctgctgGGGATAGCGACGTTAATTGCCACGGTGATGCACGTTCCGGCAGATGCTCAGGGAACACTACCCGATGCGCTGAATTTTATGAAGGTGTTCAGCAAAGGTTTGTTCACTTTCCTGGTGATTCTGGTGGGCTATAACGCCGCCCa              <  2:3051851/150‑1 (MQ=255)
                                                                                                                                         gctgGGGATAGCGACGTTAATTGCCACGGTGATGCACGTTCCGGCAGATGCTCAGGGAACACTACCCGATGCGCTGAATTTTATGAAGGTGTTCAGCAAAGGTTTGTTCACTTTCCTGGTGATTCTGGTGGGCTATAACGCCGCCCAGGc           <  2:3976488/150‑1 (MQ=255)
                                                                                                                                         gctgGGGATAGCGACGTTAATTGCCACGGTGATGCACGTTCCGGCAGATGCTCAGGGAACACTACCCGATGCGCTGAATTTTATGAAGGTGTTCAGCAAAGGTGTGTTCACTTTCCTGGTGATTCTGGTGGGCTATAACGCCGCCCAGGc           >  2:464582/1‑150 (MQ=255)
                                                                                                                                           tgGGGATAGCGACGTTAATTGCCACGGTGATGCACGTTCCGGCAGATGCTCAGGGAACACTACCCGATGCGCTGAATTTTATGAAGGTGTTCAGCAAAGGTTTGTTCACTTTCCTGGTGATTCTGGTGGGCTATAACGCCGCCCAGGcct         >  2:1600595/1‑148 (MQ=255)
                                                                                                                                             gggATAGCGACGTTAATTGCCACGGTGATGCACGTTCCGGCAGATGCTCAGGGAACACTACCCGATGCGCTGAATTTTATGAAGGTGTTCAGCAAAGGTTTGTTCACTTTCCTGGTGATTCTGGTGGGCTAGAACGCCGCCCAGGCAttg       >  2:2759733/1‑149 (MQ=255)
                                                                                                                                              ggATAGCGACGTTAATTGCCACGGTGATGCACGTTCCGGCAGATGCTCAGGGAACACTACCCGATGCGCTGAATTTTATGAAGGTGTTCAGCAAAGGTTTGTTCACTTTCCTGGTGATTCTGGTGGGCTATAACGCCGCCCAGGCATTTg      <  1:4278113/150‑1 (MQ=255)
                                                                                                                                              ggATAGCGACGTTAATTGCCACGGTGATGCACGTTCCGGCAGATGCTCAGGGAACACTACCCGATGCGCTGAATTTTATGAAGGTGTTCAGCAAAGGTTTGTTCACTTTCCTGGTGATTCTGGTGGGCTATAACGCCGCCCAGGCATTTg      >  1:38245/1‑150 (MQ=255)
                                                                                                                                                 tAGCGACGTTAATTGCCACGGTGATGCACGTTCCGGCAGATGCTCAGGGAACACTACCCGATGCGCTGAATTTTATGAAGGTGTTCAGCAAAGGTTTGTTCACTTTCCTGGTGATTCTGGTGGGCTATAACGCCGCCCAGGCATTTggcg   >  1:3462219/1‑150 (MQ=255)
                                                                                                                                                  aGCGACGTTAATTGCCACGGTGATGCACGTTCCGGCAGATGCTCAGGGAACACTACCCGATGCGCTGAATTTTATGAAGGTGTTCAGCAAAGGTTTGTTCACTTTCCTGGTGATTCTGGTGGGCTATAACGCCGCCCAGGCATTTggcgg  <  1:4865018/150‑1 (MQ=255)
                                                                                                                                                  aGCGACGTTAATTGCCACGGTGATGCACGTTCCGGCAGATGCTCAGGGAACACTACCCGATGCGCTGAATTTTATGAAGGTGTTCAGCAAAGGTTTGTTCACTTTCCTGGTGATTCTGGTGGGCTATAACGCCGCCCAGGCATTTggcgg  <  1:2248600/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                  
CAGGATGCCGCAGAAATCGCCGCCCAGAACAAACGTCAGTTAAAAGCCAAACAAACCTCCGGCGTGCAACAATTTCTCGCCAAATTCGCCACCATCTTCACGCCGCTGATCCCCGGTTTTATTGCCGCCGGTCTGCTGCTGGGGATAGCGACGTTAATTGCCACGGTGATGCACGTTCCGGCAGATGCTCAGGGAACACTACCCGATGCGCTGAATTTTATGAAGGTGTTCAGCAAAGGTTTGTTCACTTTCCTGGTGATTCTGGTGGGCTATAACGCCGCCCAGGCATTTGGCGG  >  CP009273/2540296‑2540591

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑