Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,619,125 G→T 28.1% G218C (GGT→TGT)  ppx → exopolyphosphatase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,619,1250GT28.1% 694.7 / 243.9 325G218C (GGT→TGT) ppxexopolyphosphatase
Reads supporting (aligned to +/- strand):  ref base G (116/117);  new base T (54/38);  total (170/155)
Fisher's exact test for biased strand distribution p-value = 1.75e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 329 total aligned reads displayed.

TTGTGTCAGCTTTGCCCAGCTTTATTTTCCTGGCGGGGTCATCAATAAAGAGAATTTTCAGCGCGCTCGCATGGCGGCAGCACAAAAACTGGAAACTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATAATTACCCCGGAACGTCTGGAAAAACTGGTAAAAGAAGTTTTACGTCACCGTAATTTCGCATCGCTGAGTTTACCGGGTCTTTCCGAAGAGCGG  >  CP009273/2618977‑2619274
                                                                                                                                                    |                                                                                                                                                     
ttGTGTCAGCTTTGCCCAGCTTTATTTTCCTGGCGGGGTCATCAATAAAGAGAATTTTCAGCGCGCTCGCATGGCGGCAGCACAAAAACTGGAAACTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCgg                                                                                                                                                      <  1:2409856/150‑1 (MQ=255)
   tgtCAGCTTTGCCCAGCTTTATTTTCCTGGCGGGGTCATCAATAAAGAGAATTTTCAGCGCGCTCGCATGGCGGCAGCACAAAAACTGGAAACTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTAc                                                                                                                                                   <  1:1771957/150‑1 (MQ=255)
     tCAGCTTTGCCCAGCTTTATTTTCCTGGCGGGGTCATCAATAAAGAGAATTTTCAGCGCGCTCGCATGGCGGCAGCACAAAAACTGGAAACTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCTGTACCa                                                                                                                                                 >  1:1142928/1‑150 (MQ=255)
      cAGCTTTGCCCAGCTTTATTTTCCTGGCGGGGTCATCAATAAAGAGAATTTTCAGCGCGCTCGCATGGCGGCAGCACAAAAACTGGAAACTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCAt                                                                                                                                                >  1:1687424/1‑150 (MQ=255)
       aGCTTTGCCCAGCTTTATTTTCCTGGCGGGGTCATCAATAAAGAGAATTTTCAGCGCGCTCGCATGGCGGCAGCACAAAAACTGGAAACTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATa                                                                                                                                               >  1:1263963/1‑150 (MQ=255)
       aGCTTTGCCCAGCTTTATTTTCCTGGCGGGGTCATCAATAAAGAGAATTTTCAGCGCGCTCGCATGGCGGCAGCACAAAAACTGGAAACTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATa                                                                                                                                               >  2:3466928/1‑150 (MQ=255)
       aGCTTTGCCCAGCTTTATTTTCCTGGCGGGGTCATCAATAAAGAGAATTTTCAGCGCGCTCGCATGGCGGCAGCACAAAAACTGGAAACTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATa                                                                                                                                               >  1:1981731/1‑150 (MQ=255)
          tttGCCCAGCTTTATTTTCCTGGCGGGGTCATCAATAAAGAGAATTTTCAGCGCGCTCGCATGGCGGCAGCACAAAAACTGGAAACTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATaaaa                                                                                                                                            <  2:903952/150‑1 (MQ=255)
          tttGCCCAGCTTTATTTTCCTGGCGGGGTCATCAATAAAGAGAATTTTCAGCGCGCTCGCATGGCGGCAGCACAAAAACTGGAAACTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATaaaa                                                                                                                                            >  1:3421914/1‑150 (MQ=255)
            tGCCCAGCTTTATTTTCCTGGCGGGGTCATCAATAAAGAGAATTTTCAGCGCGCTCGCATGGCGGCAGCACAAAAACTGGAAACTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATAAAAgc                                                                                                                                          >  1:902677/1‑150 (MQ=255)
               ccAGCTTTATTTTCCTGGCGGGGTCATCAATAAAGAGAATTTTCAGCGCGCTCGCATGGCGGCAGCACAAAAACTGGAAACTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATAAAAgccgc                                                                                                                                       >  1:4126765/1‑150 (MQ=255)
                   cTTTATTTTCCTGGCGGGGTCATCAATAAAGAGAATTTTCAGCGCGCTCGCATGGCGGCAGCACAAAAACTGGAAACTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCAt                                                                                                                                   <  1:1339983/150‑1 (MQ=255)
                     ttATTTTCCTGGCGGGGTCATCAATAAAGAGAATTTTCAGCGCGCTCGCATGGCGGCAGCACAAAAACTGGAAACTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGa                                                                                                                                 <  2:636030/150‑1 (MQ=255)
                            ccTGGCGGGGTCATCAATAAAGAGAATTTTCAGCGCGCTCGCATGGCGGCAGCACAAAAACTGGAAACTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCTGTACCATAAAAGCCGCCCATGAAGTGTTa                                                                                                                          >  2:3933587/1‑150 (MQ=255)
                            ccTGGCGGGGTCATCAATAAAGAGAATTTTCAGCGCGCTCGCATGGCGGCAGCACAAAAACTGGAAACTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTa                                                                                                                          <  1:4799878/150‑1 (MQ=255)
                            ccTGGCGGGGTCATCAATAAAGAGAATTTTCAGCGCGCTCGCATGGCGGCAGCACAAAAACTGGAAACTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTa                                                                                                                          >  1:2511869/1‑150 (MQ=255)
                               ggCGGGGTCATCAATAAAGAGAATTTTCAGCGCGCTCGCATGGCGGCAGCACAAAAACTGGAAACTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCTGTACCATAAAAGCCGCCCATGAAGTGTTAATg                                                                                                                       >  2:4680392/1‑150 (MQ=255)
                                gCGGGGTCATCAATAAAGAGAATTTTCAGCGCGCTCGCATGGCGGCAGCACAAAAACTGGAAACTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCTGTACCATAAAAGCCGCCCATGAAGTGTTAATgg                                                                                                                      <  1:372456/150‑1 (MQ=255)
                                 cGGGGTCATCAATAAAGAGAATTTTCAGCGCGCTCGCATGGCGGCAGCACAAAAACTGGAAACTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGa                                                                                                                     >  1:1804874/1‑150 (MQ=255)
                                 cGGGGTCATCAATAAAGAGAATTTTCAGCGCGCTCGCATGGCGGCAGCACAAAAACTGGAAACTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGa                                                                                                                     >  2:4520295/1‑150 (MQ=255)
                                  ggggTCATCAATAAAGAGAATTTTCAGCGCGCTCGCATGGCGGCAGCACAAAAACTGGAAACTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGaa                                                                                                                    <  2:4735388/150‑1 (MQ=255)
                                    ggTCATCAATAAAGAGAATTTTCAGCGCGCTCGCATGGCGGCAGCACAAAAACTGGAAACTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGaaan                                                                                                                  <  1:4594138/150‑2 (MQ=255)
                                     gTCATCAATAAAGAGAATTTTCAGCGCGCTCGCATGGCGGCAGCACAAAAACTGGAAACTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGCACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATg                                                                                                                 >  2:2079488/1‑150 (MQ=255)
                                      tcatcaATAAAGAGAATTTTCAGCGCGCTCGCATGGCGGCAGCACAAAAACTGGAAACTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCTGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATgg                                                                                                                <  2:231673/150‑1 (MQ=255)
                                        atcaATAAAGAGAATTTTCAGCGCGCTCGCATGGCGGCAGCACAAAAACTGGAAACTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCTGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGc                                                                                                              <  1:1513339/150‑1 (MQ=255)
                                         tcaATAAAGAGAATTTTCAGCGCGCTCGCATGGCGGCAGCACAAAAACTGGAAACTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCg                                                                                                             <  2:483162/150‑1 (MQ=255)
                                             tAAAGAGAATTTTCAGCGCGCTCGCATGGCGGCAGCACAAAAACTGGAAACTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGaa                                                                                                         >  1:2723620/1‑150 (MQ=255)
                                              aaaGAGAATTTTCAGCGCGCTCGCATGGCGGCAGCACAAAAACTGGAAACTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGaaa                                                                                                        >  1:436488/1‑150 (MQ=255)
                                                agagAATTTTCAGCGCGCTCGCATGGCGGCAGCACAAAAACTGGAAACTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCTGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGa                                                                                                      >  2:3053363/1‑150 (MQ=255)
                                                agagAATTTTCAGCGCGCTCGCATGGCGGCAGCACAAAAACTGGAAACTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCTGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGa                                                                                                      >  1:3290167/1‑150 (MQ=255)
                                                 gagaATTTTCAGCGCGCTCGCATGGCTGCAGCACAAAAACTGGAAACTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGAc                                                                                                     <  1:4097822/150‑1 (MQ=255)
                                                 gagaATTTTCAGCGCGCTCGCATGGCGGCAGCACAAAAACTGGAAACTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGAc                                                                                                     >  2:1875709/1‑150 (MQ=255)
                                                      ttttCAGCGCGCTCGCAGGGCGGCAGCACAAAAACTGGAAACTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCTGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGAt                                                                                                <  2:23754/150‑1 (MQ=255)
                                                          cAGCGCGCTCGCATGGCGGCAGCACAAAAACTGGAAACTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATAAtt                                                                                            <  2:1970532/150‑1 (MQ=255)
                                                          cAGCGCGCTCGCATGGCGGCAGCACAAAAACTGGAAACTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATAAtt                                                                                            >  2:1601890/1‑150 (MQ=255)
                                                           aGCGCGCTCGCATGGCGGCAGCACAAAAACTGGAAACTTTAACCTGGCAAGTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATAATTa                                                                                           <  2:5099314/150‑1 (MQ=255)
                                                                gcTCGCATGGCGGCAGCACAAAAACTGGAAACTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATAATTACCCCg                                                                                      <  2:2640222/150‑1 (MQ=255)
                                                                gcTCGCATGGCGGCAGCACAAAAACTGGAAACTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATAATTACCCCg                                                                                      <  1:4041434/150‑1 (MQ=255)
                                                                 cTCGCATGGCGGCAGCACAAAAACTGGAAACTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATAATTACCCCgg                                                                                     <  1:2615934/150‑1 (MQ=255)
                                                                     cATGGCGGCAGCACAAAAACTGGAAACTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATAATTACCCCGGAACg                                                                                 <  1:1022460/150‑1 (MQ=255)
                                                                       tGGCGGCAGCACAAAAACTGGAAACTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATAATTACCCCGGAACGTc                                                                               >  2:878223/1‑150 (MQ=255)
                                                                       tGGCGGCAGCACAAAAACTGGAAACTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATAATTACCCCGGAACGTc                                                                               >  2:4468788/1‑150 (MQ=255)
                                                                        ggcggcAGCACAAAAACTGGAAACTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCTGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATAATTACCCCGGAACGTCt                                                                              >  1:2701620/1‑150 (MQ=255)
                                                                          cggcAGCACAAAAACTGGAAACTTTAACCTGGCAATTCCGTATTCTGGGCTGGAACGTTGCAATGGGCGCTTCCTGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATAATTACCCCGGAACGTCTgg                                                                            >  2:1268508/1‑150 (MQ=255)
                                                                          cggcAGCACAAAAACTGGAAACTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCTGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATAATTACCCCGGAACGTCTgg                                                                            >  2:3281077/1‑150 (MQ=255)
                                                                          cggcAGCACAAAAACTGGAAACTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCTGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATAATTACCCCGGAACGTCTgg                                                                            >  2:113080/1‑150 (MQ=255)
                                                                             cagcaCAAAAACTGGAAACTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAACTGGGCGAGAAAGACGGGATAATTACCCCGGAACGTCTGGaaa                                                                         >  2:2827063/1‑150 (MQ=255)
                                                                             cagcaCAAAAACTGGAAACTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATAATTACCCCGGAACGTCTGGaaa                                                                         <  1:680321/150‑1 (MQ=255)
                                                                              agcaCAAAAACTGGAAACTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCTGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATAATTACCCCGGAACGTCTGGaaaa                                                                        >  2:1713969/1‑150 (MQ=255)
                                                                                cacaAAAACTTGAAACTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATTGGCGCTTCCGGTAACATAAAAGCCGCCCATGAAGTGTTAATGGAAATTGGCGAGAAAGACGGGATAATTACCCCGGAACGTCTGGAAAAAc                                                                      <  2:452263/150‑1 (MQ=255)
                                                                                cacaAAAACTGGAAACTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATAATTACCCCGGAACGTCTGGAAAAAc                                                                      >  2:1479976/1‑150 (MQ=255)
                                                                                 acaAAAACTGGAAACTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATAATTACCCCGGAACGTCTGGAAAAACt                                                                     <  2:4313396/150‑1 (MQ=255)
                                                                                 acaAAAACTGGAAACTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATAATTACCCCGGAACGTCTGGAAAAACt                                                                     >  2:4281143/1‑150 (MQ=255)
                                                                                  caAAAACTGGAAACTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCTGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATAATTACCCCGGAACGTCTGGAAAAACTg                                                                    >  2:29890/1‑150 (MQ=255)
                                                                                      aaCTGGAAACTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATAATTACCCCGGAACGTCTGGAAAAACTGGTaa                                                                >  2:2895411/1‑150 (MQ=255)
                                                                                      aaCTGGAAACTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATAATTACCCCGGAACGTCTGGAAAAACTGGTaa                                                                <  2:416960/150‑1 (MQ=255)
                                                                                        cTGGAAACTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATAATTACCCCGGAACGTCTGGAAAAACTGGTaaaa                                                              <  1:4281143/150‑1 (MQ=255)
                                                                                         tGGAAACTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATAATTACCCCGGAACGTCTGGAAAAACTGGTAAaag                                                             <  1:3112003/150‑1 (MQ=255)
                                                                                          ggAAACTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCTGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATAATTACCCCGGAACGTCTGGAAAAACTGGTAAaaga                                                            >  2:3469024/1‑150 (MQ=255)
                                                                                           gAAACTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCTGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATAATTACCCCGGAACGTCTGGAAAAACTGGTAAaagaa                                                           >  2:2742972/1‑150 (MQ=255)
                                                                                             aaCTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCTGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATAATTACCCCGGAACGTCTGGAAAAACTGGTAAAAGAAGt                                                         >  1:3550902/1‑150 (MQ=255)
                                                                                             aaCTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATAATTACCCCGGAACGTCTGGAAAAACTGGTAAAAGAAGt                                                         <  2:3595013/150‑1 (MQ=255)
                                                                                             aaCTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATAATTACCCCGGAACGTCTGGAAAAACTGGTAAAAGAAGt                                                         >  1:3671266/1‑150 (MQ=255)
                                                                                             aaCTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATAATTACCCCGGAACGTCTGGAAAAACTGGTAAAAGAAGt                                                         >  1:2336869/1‑150 (MQ=255)
                                                                                               cTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCTGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATAATTACCCCGGAACGTCTGGAAAAACTGGTAAAAGAAGttt                                                       >  1:532536/1‑150 (MQ=255)
                                                                                               cTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCTGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATAATTACCCCGGAACGTCTGGAAAAACTGGTAAAAGAAGttt                                                       <  1:740624/150‑1 (MQ=255)
                                                                                                tttAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATAATTACCCCGGAACGTCTGGAAAAACTGGTAAAAGAAGtttt                                                      <  2:151451/150‑1 (MQ=255)
                                                                                                  tAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATAATTACCCCGGAACGTCTGGAAAAACTGGTAAAAGAAGTTTTAc                                                    <  1:1422018/150‑1 (MQ=255)
                                                                                                     ccTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATAATTACCCCGGAACGTCTGGAAAAACTGGTAAAAGAAGTTTTACGTc                                                 <  1:4858847/150‑1 (MQ=255)
                                                                                                     ccTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATAATTACCCCGGAACGTCTGGAAAAACTGGTAAAAGAAGTTTTACGTc                                                 <  2:703752/150‑1 (MQ=255)
                                                                                                      cTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATAATTACCCCGGAACGTCTGGAAAAACTGGTAAAAGAAGTTTTACGTCa                                                <  2:2117431/150‑1 (MQ=255)
                                                                                                       tGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCTGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATAATTACCCCGGAACGTCTGGAAAAACTGGTAAAAGAAGTTTTACGTCAc                                               >  1:3536178/1‑150 (MQ=255)
                                                                                                           aaTTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCTGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATAATTACCCCGGAACGTCTGGAAAAACTGGTAAAAGAAGTTTTACGTCACCGTa                                           <  2:176264/150‑1 (MQ=255)
                                                                                                            aTTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATAATTACCCCGGAACGTCTGGAAAAACTGGTAAAAGAAGTTTTACGTCACCGTaa                                          <  1:2900048/150‑1 (MQ=255)
                                                                                                             ttCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATAATTACCCCGGAACGTCTGGAAAAACTGGTAAAAGAAGTTTTACGTCACCGTAAt                                         <  2:4025159/150‑1 (MQ=255)
                                                                                                              tCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATAATTACCCCGGAACGTCTGGAAAAACTGGTAAAAGAAGTTTTACGTCACCGTAAtt                                        >  1:5134529/1‑150 (MQ=255)
                                                                                                                cGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATAATTACCCCGGAACGTCTGGAAAAACTGGTAAAAGAAGTTTTACGTCACCGTAATTTc                                      <  1:4494362/150‑1 (MQ=255)
                                                                                                                cGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATAATTACCCCGGAACGTCTGGAAAAACTGGTAAAAGAAGTTTTACGTCACCGTAATTTc                                      <  2:1864692/150‑1 (MQ=255)
                                                                                                                  tATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCTGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATAATTACCCCGGAACGTCTGGAAAAACTGGTAAAAGAAGTTTTACGTCACCGTAATTTCGc                                    >  2:3240036/1‑150 (MQ=255)
                                                                                                                   aTTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATAATTACCCCGGAACGTCTGGAAAAACTGGTAAAAGAAGTTTTACGTCACCGTAATTTCGCa                                   <  2:3465070/150‑1 (MQ=255)
                                                                                                                     tCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATAATTACCCCGGAACGTCTGGAAAAACTGGTAAAAGAAGTTTTACGTCACCGTAATTTCGCATc                                 <  2:2397267/150‑1 (MQ=255)
                                                                                                                     tCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATAATTACCCCGGAACGTCTGGAAAAACTGGTAAAAGAAGTTTTACGTCACCGTAATTTCGCATc                                 >  1:2060661/1‑150 (MQ=255)
                                                                                                                     tCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATAATTACCCCGGAACGTCTGGAAAAACTGGTAAAAGAAGTTTTACGTCACCGTAATTTCGCATc                                 <  1:929203/150‑1 (MQ=255)
                                                                                                                       aGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATAATTACCCCGGAACGTCTGGAAAAACTGGTAAAAGAAGTTTTACGTCACCGTAATTTCGCATCGc                               >  2:800792/1‑150 (MQ=255)
                                                                                                                        gggCTGGAACGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATAATTACCCCGGAACGTCTGGAAAAACTGGTAAAAGAAGTTTTACGTCACCGTAATTTCGCATCGCt                              >  1:1603737/1‑150 (MQ=255)
                                                                                                                          gCTGGAACGTTGCAATGGGCGCTTCCTGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATAATTACCCCGGAACGTCTGGAAAAACTGGTAAAAGAAGTTTTACGTCACCGTAATTTCGCATCGCTGa                            >  2:441944/1‑150 (MQ=255)
                                                                                                                             ggAACGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATAATTACCCCGGAACGTCTGGAAAAACTGGTAAAAGAAGTTTTACGTCACCGTAATTTCGCATCGCTGAGtt                         <  1:4707956/150‑1 (MQ=255)
                                                                                                                             ggAACGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATAATTACCCCGGAACGTCTGGAAAAACTGGTAAAAGAAGTTTTACGTCACCGTAATTTCGCATCGCTGAGtt                         >  1:2199781/1‑150 (MQ=255)
                                                                                                                                       aaTGGGCGCTTCCTGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATAATTACCCCGGAACGTCTGGAAAAACTGGTAAAAGAAGTTTTACGTCACCGTAATTTCGCATCGCTGAGTTTACCGGGTCt               >  2:3233549/1‑150 (MQ=255)
                                                                                                                                        aTGGGCGCTTCCTGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATAATTACCCCGGAACGTCTGGAAAAACTGGTAAAAGAAGTTTTACGTCACCGTAATTTCGCATCGCTGAGTTTACCGGGTCtt              >  2:4582402/1‑150 (MQ=255)
                                                                                                                                         tGGGCGCTTCCTGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATAATTACCCCGGAACGTCTGGAAAAACTGGTAAAAGAAGTTTTACGTCACCGTAATTTCGCATCGCTGAGTTTACCGGGTCttt             <  1:396433/150‑1 (MQ=255)
                                                                                                                                         tGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATAATTACCCCGGAACGTCTGGAAAAACTGGTAAAAGAAGTTTTACGTCACCGTAATTTCGCATCGCTGAGTTTACCGGGTCttt             >  1:402439/1‑150 (MQ=255)
                                                                                                                                         tGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATAATTACCCCGGAACGTCTGGAAAAACTGGTAAAAGAAGTTTTACGTCACCGTAATTTCGCATCGCTGAGTTTACCGGGTCttt             <  1:1875673/150‑1 (MQ=255)
                                                                                                                                           ggCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATAATTACCCCGGAACGTCTGGAAAAACTGGTAAAAGAAGTTTTACGTCACCGTAATTTCGCATCGCTGAGTTTACCGGGTCTTTcc           >  1:4169259/1‑150 (MQ=255)
                                                                                                                                             cgcTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATAATTACCCCGGAACGTCTGGAAAAACTGGTAAAAGAAGTTTTACGTCACCGTAATTTCGCATCGCTGAGTTTACCGGGTCTTTCCGa         <  2:106590/150‑1 (MQ=255)
                                                                                                                                              gcTTCCGGTACCATAAAAGCCGCCCCTGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATAATTACCCCGGAACGTCTGGAAAAACTGGTAAAAGAAGTTTTACGTCACCGTAATTTCGCATCGCTGAGTTTACCGGGTCTTTCCGaa        <  2:3719111/150‑1 (MQ=255)
                                                                                                                                                ttCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATAATTACCCCGGAACGTCTGGAAAAACTGGTAAAAGAAGTTTTACGTCACCGTAATTTCGCATCGCTGAGTTTACCGGGTCTTTCCGAaga      >  1:2503077/1‑150 (MQ=255)
                                                                                                                                                 tCCTGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATAATTACCCCGGAACGTCTGGAAAAACTGGTAAAAGAAGTTTTACGTCACCGTAATTTCGCATCGCTGAGTTTACCGGGTCTTTCCGAagag     <  2:3194883/150‑1 (MQ=255)
                                                                                                                                                 tCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATAATTACCCCGGAACGTCTGGAAAAACTGGTAAAAGAAGTTTTACGTCACCGTAATTTCGCATCGCTGAGTTTACCGGGTCTTTCCGAagag     <  1:4968928/150‑1 (MQ=255)
                                                                                                                                                  ccGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATAATTACCCCGGAACGTCTGGAAAAACTGGTAAAAGAAGTTTTACGTCACCGTAATTTCGCATCGCTGAGTTTACCGGGTCTTTCCGAAGAGc    >  2:2218865/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
TTGTGTCAGCTTTGCCCAGCTTTATTTTCCTGGCGGGGTCATCAATAAAGAGAATTTTCAGCGCGCTCGCATGGCGGCAGCACAAAAACTGGAAACTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATAATTACCCCGGAACGTCTGGAAAAACTGGTAAAAGAAGTTTTACGTCACCGTAATTTCGCATCGCTGAGTTTACCGGGTCTTTCCGAAGAGCGG  >  CP009273/2618977‑2619274

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑