Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,619,563 C→A 10.8% R364R (CGA→AGA)  ppx → exopolyphosphatase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,619,5630CA10.8% 1350.9 / 91.6 380R364R (CGA→AGA) ppxexopolyphosphatase
Reads supporting (aligned to +/- strand):  ref base C (159/180);  new base A (18/23);  total (177/203)
Fisher's exact test for biased strand distribution p-value = 7.43e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.90e-01

Only 100 of 384 total aligned reads displayed.

TGCGTAGTCGCACCGCCAGCAGCCTCGCCAACCAGTATCACATCGACAGCGAACAGGCCCGACGGGTGCTGGATACCACTATGCAAATGTACGAACAGTGGCGGGAACAGCAACCGAAGCTGGCGCATCCGCAACTGGAGGCGCTACTGCGATGGGCCGCCATGCTGCATGAGGTCGGGTTGAATATCAACCACAGCGGTTTGCATCGCCACTCCGCTTATATTCTGCAAAACAGTGACTTGCCGGGTTTTAATCAGGAACAGCAGCTGATGATGGCGACACTGGTGCGCTATCACCGT  >  CP009273/2619414‑2619712
                                                                                                                                                     |                                                                                                                                                     
tgCGTAGTCGCACCGCCAGCAGCCTCGCCAACCAGTATCACATCGACAGCGAACAGGCCCGACGGGTGCTGGATACCACTATGCAAATGTACGAACAGTGGCGGGAACAGCAACCGAAGCTGGCGCATCCGCAACTGGAGGCGCTACTGc                                                                                                                                                       <  1:3448202/150‑1 (MQ=255)
 gCGTAGTCGCACCGCCAGCAGCCTCGCCAACCAGTATCACATCGACAGCGAACAGGCCCGACGGGTGCTGGATACCACTATGCAAATGTACGAACAGTGGCGGGAACAGCAACCGAAGCTGGCGCATCCGCAACTGGAGGCGCTACTGCg                                                                                                                                                      >  2:3123256/1‑150 (MQ=255)
   gTAGTCGCACCGCCAGCAGCCTCGCCAACCAGTATCACATCGACAGCGAACAGGCCCGACGGGTGCTGGATACCACTATGCAAATGTACGAACAGTGGCGGGAACAGCAACCGAAGCTGGCGCATCCGCAACTGGAGGCGCTACTGCGAt                                                                                                                                                    >  1:2005051/1‑150 (MQ=255)
     aGTCGCACCGCCAGCAGCCTCGCCAACCAGTATCACATCGACAGCGAACAGGCCCGACGGGTGCTGGATACCACTATGCAAATGTACGAACAGTGGCGGGAACAGCAACCGAAGCTGGCGCATCCGCAACTGGAGGCGCTACTGCGATgg                                                                                                                                                  >  2:1684497/1‑150 (MQ=255)
       tCGCACCGCCAGCAGCCTCGCCAACCAGTATCACATCGACAGCGAACAGGCCCGACGGGTGCTGGATACCACTATGCAAATGTACGAACAGTGGCGGGAACAGCAACCGAAGCTGGCGCATCCGCAACTGGAGGCGCTACTGAGATGGgc                                                                                                                                                <  1:1274128/150‑1 (MQ=255)
        cGCACCGCCAGCAGCCTCGCCAACCAGTATCACATCGACAGCGAACAGGCCCGACGGGTGCTGGATACCACTATGCAAATGTACGAACAGTGGCGGGAACAGCAACCGAAGCTGGCGCATCCGCAACTGGAGGCGCTACTGCGATGGgcc                                                                                                                                               >  1:3834380/1‑150 (MQ=255)
        cGCACCGCCAGCAGCCTCGCCAACCAGTATCACATCGACAGCGAACAGGCCCGACGGGTGCTGGATACCACTATGCAAATGTACGAACAGTGGCGGGAACAGCAACCGAAGCTGGCGCATCCGCAACTGGAGGCGCTACTGCGATGGgcc                                                                                                                                               <  1:2583759/150‑1 (MQ=255)
          cACCGCCAGCAGCCTCGCCAACCAGTATCACATCGACAGCGAACAGGCCCGACGGGTGCTGGATACCACTATGCAAATGTACGAACAGTGGCGGGAACAGCAACCGAAGCTGGCGCATCCGCAACTGGAGGCGCTACTGCGATGGgccgc                                                                                                                                             <  1:3468802/150‑1 (MQ=255)
           aCCGCCAGCAGCCTCGCCAACCAGTATCACATCGACAGCGAACAGGCCCGACGGGTGCTGGATACCACTATGCAAATGTACGAACAGTGGCGGGAACAGCAACCGAAGCTGGCGCATCCGCAACTGGAGGCGCTACTGCGATGGgccgcc                                                                                                                                            <  1:2106245/150‑1 (MQ=255)
           aCCGCCAGCAGCCTCGCCAACCAGGATCACATCGACAGCGACCAGGCCCGACGGGTGCTGGATACCACTATGCAACGGTACGAACAGTGGCGGGAACAGCAACCGAAGCTGGCGCATCCGCAACTGGAGGCGCTACTGCGATGGgccgcc                                                                                                                                            <  2:2470957/150‑1 (MQ=255)
             cGCCAGCAGCCTCGCCAACCAGTATCACATCGACAGCGAACAGGCCCGACGGGTGCTGGATACCACTATGCAAATGTACGAACAGTGGCGGGAACAGCAACCGAAGCTGGCGCATCCGCAACTGGAGGCGCTACTGCGATGGGCCGCCAt                                                                                                                                          <  1:4582485/150‑1 (MQ=255)
             cGCCAGCAGCCTCGCCAACCAGTACCACATCGACAGCGAACAGGCCCGACGGGTGCTGGATACCACTATGCAAATGTACGAACAGTGGCGGGAACAGCAACCGAAGCTGGCGCATCCGCAACTGGAGGCGCTACTGCGATGGGCCGCCAt                                                                                                                                          >  2:2762558/1‑150 (MQ=255)
              gCCAGCAGCCTCGCCAACCAGTATCACATCGACAGCGAACAGGCCCGACGGGTGCTGGATACCACTATGCAAATGTACGAACAGTGGCGGGAACAGCAACCGAAGCTGGCGCATCCGCAACTGGAGGCGCTACTGCGATGGGCCGCCAtg                                                                                                                                         <  2:930699/150‑1 (MQ=255)
                cagcagCCTCGCCAACCAGTATCACATCGACAGCGAACAGGCCCGACGGGTGCTGGATACCACTATGCAAATGTACGAACAGTGGCGGGAACAGCAACCGAAGCTGGCGCATCCGCAACTGGAGGCGCTACTGCGATGGGCCGCCAtgct                                                                                                                                       <  1:247409/150‑1 (MQ=255)
                   cagcCTCGCCAACCAGTATCACATCGACAGCGAACAGGCCCGACGGGTGCTGGATACCACTATGCAAATGTACGAACAGTGGCGGGAACAGCAACCGAAGCTGGCGCATCCGCAACTGGAGGCGCTACTGCGATGGGCCGCCATGCTGCa                                                                                                                                    >  1:3648018/1‑150 (MQ=255)
                    agcCTCGCCAACCAGTATCACATCGACAGCGAACAGGCCCGACGGGTGCTGGATACCACTATGCAAATGTACGAACAGTGGCGGGAACAGCAACCGAAGCTGGCGCATCCGCAACTGGAGGCGCTACTGCGATGGGCCGCCATGCTGCAt                                                                                                                                   >  1:953026/1‑150 (MQ=255)
                    agcCTCGCCAACCAGTATCACATCGACAGCGAACAGGCCCGACGGGTGCTGGATACCACTATGCAAATGTACGAACAGTGGCGGGAACAGCAACCGAAGCTGGCGCATCCGCAACTGGAGGCGCTACTGCGATGGGCCGCCATGCTGCAt                                                                                                                                   >  1:3198178/1‑150 (MQ=255)
                       cTCGCCAACCAGTATCACATCGACAGCGAACAGGCCCGACGGGTGCTGGATACCACTATGCAAATGTACGAACAGTGGCGGGAACAGCAACCGAAGCTGGCGCATCCGCAACTGGAGGCGCTACTGCGATGGGCCGCCATGCTGCATGAg                                                                                                                                >  1:819366/1‑150 (MQ=255)
                        tCGCCAACCAGTATCACATCGACAGCGAACAGGCCCGACGGGTGCTGGATACCACTATGCAAATGTACGAACAGTGGCGGGAACAGCAACCGAAGCTGGCGCATCCGCAACTGGAGGCGCTACTGCGATGGGCCGCCATGCTGCATGAgg                                                                                                                               >  1:586784/1‑150 (MQ=255)
                         cGCCAACCAGTATCACATCGACAGCGAACAGGCCCGACGGGTGCTGGATACCACTATGCAAATGTACGAACAGTGGCGGGAACAGCAACCGAAGCTGGCGCATCCGCAACTGGAGGCGCTACTGCGATGGGCCGCCATGCTGCATGAGGt                                                                                                                              >  2:338604/1‑150 (MQ=255)
                         cGCCAACCAGTATCACATCGACAGCGAACAGGCCCGACGGGTGCTGGATACCACTATGCAAATGTACGAACAGTGGCGGGAACAGCAACCGAAGCTGGCGCATCCGCAACTGGAGGCGCTACTGCGATGGGCCGCCATGCTGCATGAGGt                                                                                                                              >  2:4381771/1‑150 (MQ=255)
                          gCCAACCAGTATCACATCGACAGCGAACAGGCCCGACGGGTGCTGGATACCACTATGCAAATGTACGAACAGTGGCGGGAACAGCAACCGAAGCTGGCGCATCCGCAACTGGAGGCGCTACTGCGATGGGCCGCCATGCTGCATGAGGTc                                                                                                                             >  1:290776/1‑150 (MQ=255)
                          gCCAACCAGTATCACATCGACAGCGAACAGGCCCGACGGGTGCTGGATACCACTATGCAAATGTACGAACAGTGGCGGGAACAGCAACCGAAGCTGGCGCATCCGCAACTGGAGGCGCTACTGCGATGGGCCGCCATGCTGCATGAGGTc                                                                                                                             >  1:4292880/1‑150 (MQ=255)
                            cAACCAGTATCACATCGACAGCGAACAGGCCCGACGGGTGCTGGATACCACTATGCAAATGTACGAACAGTGGCGGGAACAGCAACCGAAGCTGGCGCATCCGCAACTGGAGGCGCTACTGAGATGGGCCGCCATGCTGCATGAGGTCgg                                                                                                                           >  1:3229988/1‑150 (MQ=255)
                             aaCCAGTATCACATCGACAGCGAACAGGCCCGACGGGTGCTGGATACCACTATGCAAATGTACGAACAGTGGCGGGAACAGCAACCGAAGCTGGCGCATCCGCAACTGGAGGCGCTACTGCGATGGGCCGCCATGCTGCATGAGGTCggg                                                                                                                          <  2:4048568/150‑1 (MQ=255)
                               ccAGTATCACATCGACAGCGAACAGGCCCGACGGGTGCTGGATACCACTATGCAAATGTACGAACAGTGGCGGGAACAGCAACCGAAGCTGGCGCATCCGCAACTGGAGGCGCTACTGCGATGGGCCGCCATGCTGCATGAGGTCGGGtt                                                                                                                        >  1:4979112/1‑150 (MQ=255)
                               ccAGTATCACATCGACAGCGAACAGGCCCGACGGGTGCTGGATACCACTATGCAAATGTACGAACAGTGGCGGGAACAGCAACCGAAGCTGGCGCATCCGCAACTGGAGGCGCTACTGCGATGGGCCGCCATGCTGCATGAGGTCGGGtt                                                                                                                        <  2:1823295/150‑1 (MQ=255)
                                 aGTATCACATCGACAGCGAACAGGCCCGACGGGTGCTGGATACCACTATGCAAATGTACGAACAGTGGCGGGAACAGCAACCGAAGCTGGCGCATCCGCAACTGGAGGCGCTACTGCGATGGGCCGCCATGCTGCATGAGGTCGGGTTGa                                                                                                                      <  2:4540326/150‑1 (MQ=255)
                                    aTCACATCGACAGCGAACAGGCCCGACGGGTGCTGGATACCACTAGGCAAATGTACGAACAGTGGCGGGAACAGCAACCGAAGCTGGCGCATCCGCAACTGGAGGCGCTACTGAGATGGGCCGCCATGCTGCATGAGGTCGGGTTGAata                                                                                                                   <  2:4329620/150‑1 (MQ=255)
                                        caTCGACAGCGAACAGGCCCGACGGGTGCTGGATACCACTATGCAAATGTACGAACAGTGGCGGGAACAGCAACCGAAGCTGGCGCATCCGCAACTGGAGGCGCTACTGAGATGGGCCGCCATGCTGCATGAGGTCGGGTTGAATATCaa                                                                                                               <  2:693305/150‑1 (MQ=255)
                                         aTCGACAGCGAACAGGCCCGACGGGTGCTGGATACCACTATGCAAATGTACGAACAGTGGCGGGAACAGCAACCGAAGCTGGCGCATCCGCAACTGGAGGCGCTACTGCGATGGGCCGCCATGCTGCATGAGGTCGGGTTGAATATCAAc                                                                                                              <  1:5082431/150‑1 (MQ=255)
                                          tCGACAGCGAACAGGCCCGACGGGTGCTGGATACCACTATGCAAATGTACGAACAGTGGCGGGAACAGCAACCGAAGCTGGCGCATCCGCAACTGGAGGCGCTACTGCGATGGGCCGCCATGCTGCATGAGGTCGGGTTGAATATCAAcc                                                                                                             >  1:114483/1‑150 (MQ=255)
                                            gACAGCGAACAGGCCCGACGGGTGCTGGATACCACTATGCAAATGTACGAACAGTGGCGGGAACAGCAACCGAAGCTGGCGCATCCGCAACTGGAGGCGCTACTGCGATGGGCCGCCATGCTGCATGAGGTCGGGTTGAATATCAACcac                                                                                                           <  1:3700619/150‑1 (MQ=255)
                                            gACAGCGAACAGGCCCGACGGGTGCTGGATACCACTATGCAAATGTACGAACAGGGGCGGGAACAGCAACCGAAGCTGGCGCATCCGCAACTGGAGGCGCTACTGCGATGGGCCGCCATGCTGCATGAGGTCGGGTTGAATATCAACcac                                                                                                           <  1:1046245/150‑1 (MQ=255)
                                             aCAGCGAACAGGCCCGACGGGTGCTGGATACCACTATGCAAATGTACGAACAGTGGCGGGAACAGCAACCGAAGCTGGCGCATCCGCAACTGGAGGCGCTACTGCGATGGGCCGCCATGCTGCATGAGGTCGGGTTGAATATCAACcaca                                                                                                          <  1:1170879/150‑1 (MQ=255)
                                                 cGAACAGGCCCGACGGGTGCTGGATACCACTCTGCAAAGGTACGAACAGTGGCGGGAACAGCAACCGAAGCTGGCGCATCCGCAACTGGAGGCGCTACTGCGATGGGCCGCCATGCTGCATGAGGTCGGGTTGAATATCAACCACAGCgg                                                                                                      <  2:4652681/150‑1 (MQ=255)
                                                  gAACAGGCCCGACGGGTGCTGGATACCACTATGCAAATGTACGAACAGTGGCGGGAACAGCAACCGAAGCTGGCGCATCCGCAACTGGAGGCGCTACTGCGATGGGCCGCCATGCTGCATGAGGTCGGGTTGAATATCAACCACAGCGGt                                                                                                     >  2:233819/1‑150 (MQ=255)
                                                  gAACAGGCCCGACGGGTGCTGGATACCACTATGCAAATGTACGAACAGTGGCGGGAACAGCAACCGAAGCTGGCGCATCCGCAACTGGAGGCGCTACTGCGATGGGCCGCCATGCTGCATGAGGTCGGGTTGAATATCAACCACAGCGGt                                                                                                     >  1:2274815/1‑150 (MQ=255)
                                                   aaCAGGCCCGACGGGTGCTGGATACCACTATGCAAATGTACGAACAGTGGCGGGAACAGCAACCGAAGCTGGCGCATCCGCAACTGGAGGCGCTACTGAGATGGGCCGCCATGCTGCATGAGGTCGGGTTGAATATCAACCACAGCGGtt                                                                                                    <  2:1428583/150‑1 (MQ=255)
                                                   aaCAGGCCCGACGGGTGCTGGATACCACTATGCAAATGTACGAACAGTGGCGGGAACAGCAACCGAAGCTGGCGCATCCGCAACTGGAGGCGCTACTGAGATGGGCCGCCATGCTGCATGAGGTCGGGTTGAATATCAACCACAGCGGtt                                                                                                    >  1:1665022/1‑150 (MQ=255)
                                                     cAGGCCCGACGGGTGCTGGATACCACTATGCAAATGTACGAACAGTGGCGGGAACAGCAACCGAAGCTGGCGCATCCGCAACTGGAGGCGCTACTGCGATGGGCCGCCATGCTGCATGAGGTCGGGTTGAATATCAACCACAGCGGTTTg                                                                                                  <  1:1477956/150‑1 (MQ=255)
                                                       ggCCCGACGGGTGCTGGATACCACTATGCAAATGTACGAACAGTGGCGGGAACAGCAACCGAAGCTGGCGCATCCGCAACTGGAGGCGCTACTGCGATGGGCCGCCATGCTGCATGAGGTCGGGTTGAATATCAACCACAGCGGTTTGCa                                                                                                >  2:3890798/1‑150 (MQ=255)
                                                         cccGACGGGTGCTGGATACCACTATGCAAATGTACGAACAGTGGCGGGAACAGCAACCGAAGCTGGCGCATCCGCAACTGGAGGCGCTACTGCGATGGGCCGCCATGCTGCATGAGGTCGGGTTGAATATCAACCACAGCGGTTTGCATc                                                                                              <  2:2133197/150‑1 (MQ=255)
                                                              cGGGTGCTGGATACCACTATGCAAATGTACGAACAGTGGCGGGAACAGCAACCGAAGCTGGCGCATCCGCAACTGGAGGCGCTACTGCGATGGGCCGCCATGCTGCATGAGGTCGGGTTGAATATCAACCACAGCGGTTTGCATCGCCAc                                                                                         >  2:1299262/1‑150 (MQ=255)
                                                              cGGGTGCTGGATACCACTATGCAAATGTACGAACAGTGGCGGGAACAGCAACCGAAGCTGGCGCATCCGCAACTGGAGGCGCTACTGCGATGGGCCGCCATGCTGCATGAGGTCGGGTTGAATATCAACCACAGCGGTTTGCATCGCCAc                                                                                         >  1:4028785/1‑150 (MQ=255)
                                                               gggTGCTGGATACCACTATGCAAATGTACGAACAGTGGCGGGAACAGCAACCGAAGCTGGCGCATCCGCAACTGGAGGCGCTACTGCGATGGGCCGCCATGCTGCATGAGGTCGGGTTGAATATCAACCACAGCGGTTTGCATCGCCACt                                                                                        >  1:91789/1‑150 (MQ=255)
                                                                 gTGCTGGATACCACTATGCAAATGTACGAACAGTGGCGGGAACAGCAACCGAAGCTGGCGCATCCGCAACTGGAGGCGCTACTGCGATGGGCCGCCATGCTGCATGAGGTCGGGTTGAATATCAACCACAGCGGTTTGCATCGCCACTcc                                                                                      >  1:683402/1‑150 (MQ=255)
                                                                  tGCTGGATACCACTATGCAAATGTACGAACAGTGGCGGGAACAGCAACCGAAGCTGGCGCATCCGCAACTGGAGGCGCTACTGCGATGGGCCGCCATGCTGCATGAGGTCGGGTTGAATATCAACCACAGCGGTTTGCATCGCCACTCCg                                                                                     >  2:787807/1‑150 (MQ=255)
                                                                  tGCTGGATACCACTATGCAAATGTACGAACAGTGGCGGGAACAGCAACCGAAGCTGGCGCATCCGCAACTGGAGGCGCTACTGCGATGGGCCGCCATGCTGCATGAGGTCGGGTTGAATATCAACCACAGCGGTTTGCATCGCCACTCCg                                                                                     <  2:3080527/150‑1 (MQ=255)
                                                                    cTGGATACCACTATGCAAATGTACGAACAGTGGCGGGAACAGCAACCGAAGCTGGCGCATCCGCAACTGGAGGCGCTACTGCGATGGGCCGCCATGCTGCATGAGGTCGGGTTGAATATCAACCACAGCGGTTTGCATCGCCACTCCGCt                                                                                   <  2:725040/150‑1 (MQ=255)
                                                                    cTGGATACCACTATGCAAATGTACGAACAGTGGCGGGAACAGCAACCGAAGCTGGCGCATCCGCAACTGGAGGCGCTACTGCGATGGGCCGCCATGCTGCATGAGGTCGGGTTGAATATCAACCACAGCGGTTTGCATCGCCACTCCGCt                                                                                   >  2:2044343/1‑150 (MQ=255)
                                                                     tGGATACCACTATGCAAATGTACGAACAGTGGCGGGAACAGCAACCGAAGCTGGCGCATCCGCAACTGGAGGCGCTACTGCGATGGGCCGCCATGCTGCATGAGGTCGGGTTGAATATCAACCACAGCGGTTTGCATCGCCACTCCGCtt                                                                                  >  1:4733966/1‑150 (MQ=255)
                                                                      ggATACCACTATGCAAATGTACGAACAGTGGCGGGAACAGCAACCGAAGCTGGCGCATCCGCAACTGGAGGCGCTACTGCGATGGGCCGCCATGCTGCATGAGGTCGGGTTGAATATCAACCACAGCGGTTTGCATCGCCACTCCGCTta                                                                                 >  2:1157499/1‑150 (MQ=255)
                                                                      ggATACCACTATGCAAATGTACGAACAGTGGCGGGAACAGCAACCGAAGCTGGCGCATCCGCAACTGGAGGCGCTACTGCGATGGGCCGCCATGCTGCATGAGGTCGGGTTGAATATCAACCACAGCGGTTTGCATCGCCACTCCGCTta                                                                                 <  1:4340127/150‑1 (MQ=255)
                                                                       gATACCACTATGCAAATGTACGAACAGTGGCGGGAACAGCAACCGAAGCTGGCGCATCCGCAACTGGAGGCGCTACTGCGATGGGCCGCCATGCTGCATGAGGTCGGGTTGAATATCAACCACAGCGGTTTGCATCGCCACTCCGCTtat                                                                                >  1:4861109/1‑150 (MQ=255)
                                                                        aTACCACTATGCAAATGTACGAACAGTGGCGGGAACAGCAACCGAAGCTGGCGCATCCGCAACTGGAGGCGCTACTGCGATGGGCCGCCATGCTGCATGAGGTCGGGTTGAATATCAACCACAGCGGTTTGCATCGCCACTCCGCTtata                                                                               >  1:3927321/1‑150 (MQ=255)
                                                                         tACCACTATGCAAATGTACGAACAGTGGCGGGAACAGCAACCGAAGCTGGCGCATCCGCAACTGGAGGCGCTACTGCGATGGGCCGCCATGCTGCATGAGGTCGGGTTGAATATCAACCACAGCGGTTTGCATCGCCACTCCGCTTatat                                                                              <  1:5130284/150‑1 (MQ=255)
                                                                           ccACTATGCAAATGTACGAACAGTGGCGGGAACAGCAACCGAAGCTGGCGCATCCGCAACTGGAGGCGCTACTGAGATGGGCCGCCATGCTGCATGAGGTCGGGTTGAATATCAACCACAGCGGTTTGCATCGCCACTCCGCTTATATTc                                                                            <  1:3284618/150‑1 (MQ=255)
                                                                            cACTATGCAAATGTACGAACAGTGGCGGGAACAGCAACCGAAGCTGGCGCATCCGCAACTGGAGGCGCTACTGCGATGGGCCGCCATGCTGCATGAGGTCGGGTTGAATATCAACCACAGCGGTTTGCATCGCCACTCCGCTTATATTCt                                                                           >  2:3939797/1‑150 (MQ=255)
                                                                             aCTATGCAAATGTACGAACAGTGGCGGGAACAGCAACCGAAGCTGGCGCATCCGCAACTGGAGGCGCTACTGCGATGGGCCGCCATGCTGCATGAGGTCGGGTTGAATATCAACCACAGCGGTTTGCATCGCCACTCCGCTTATATTCTg                                                                          >  2:1390259/1‑150 (MQ=255)
                                                                              cTATGCAAATGTACGAACAGTGGCGGGAACAGCAACCGAAGCTGGCGCATCCGCAACTGGAGGCGCTACTGCGATGGGCCGCCATGCTGCATGAGGTCGGGTTGAATATCAACCACAGCGGTTTGCATCGCCACTCCGCTTATATTCTGc                                                                         <  1:2772532/150‑1 (MQ=255)
                                                                                aTGCAAATGTACGAACAGTGGCGGGAACAGCAACCGAAGCTGGCGCATCCGCAACTGGAGGCGCTACTGCGATGGGCCGCCATGCTGCATGAGGTCGGGTTGAATATCAACCACAGCGGTTTGCATCGCCACTCCGCTTATATTCTGCaa                                                                       >  2:1030273/1‑150 (MQ=255)
                                                                                        gTACGAACAGTGGCGGGAACAGCAACCGAAGCTGGCGCATCCGCAACTGGAGGCGCTACTGCGATGGGCCGCCATGCTGCATGAGGTCGGGTTGAATATCAACCACAGCGGTTTGCATCGCCACTCCGCTTATATTCTGCAAAACAGTGa                                                               >  1:1933677/1‑150 (MQ=255)
                                                                                        gTACGAACAGTGGCGGGAACAGCAACCGAAGCTGGCGCATCCGCAACTGGAGGCGCTACTGCGATGGGCCGCCATGCTGCATGAGGTCGGGTTGAATATCAACCACAGCGGTTTGCATCGCCACTCCGCTTATATTCTGCAAAACAGTGa                                                               >  1:1399294/1‑150 (MQ=255)
                                                                                         tACGAACAGTGGCGGGAACAGCAACCGAAGCTGGCGCATCCGCAACTGGAGGCGCTACTGCGATGGGCCGCCATGCTGCATGAGGTCGGGTTGAATATCAACCACAGCGGTTTGCATCGCCACTCCGCTTATATTCTGCAAAACAGTGAc                                                              <  2:2597422/150‑1 (MQ=255)
                                                                                           cGAACAGTGGCGGGAACAGCAACCGAAGCTGGCGCATCCGCAACTGGAGGCGCTACTGCGATGGGCCGCCATGCTGCATGAGGTCGGGTTGAATATCAACCACAGCGGTTTGCATCGCCACTCCGCTTATATTCTGCAAAACAGTGACtt                                                            >  2:3903263/1‑150 (MQ=255)
                                                                                            gAACAGTGGCGGGAACAGCAACCGAAGCTGGCGCATCCGCAACTGGAGGCGCTACTGCGATGGGCCGCCATGCTGCATGAGGTCGGGTTGAATATCAACCACAGCGGTTTGCATCGCCACTCCGCTTATATTCTGCAAAACAGTGACTTg                                                           <  2:53115/150‑1 (MQ=255)
                                                                                              aCAGTGGCGGGAACAGCAACCGAAGCTGGCGCATCCGCAACTGGAGGCGCTACTGCGATGGGCCGCCATGCTGCATGAGGTCGGGTTGAATATCAACCACAGCGGTTTGCATCGCCACTCCGCTTATATTCTGCAAAACAGTGACTTGcc                                                         >  2:1770627/1‑150 (MQ=255)
                                                                                              aCAGTGGCGGGAACAGCAACCGAAGCTGGCGCATCCGCAACTGGAGGCGCTACTGCGATGGGCCGCCATGCTGCATGAGGTCGGGTTGAATATCAACCACAGCGGTTTGCATCGCCACTCCGCTTATATTCTGCAAAACAGTGACTTGcc                                                         <  1:3628771/150‑1 (MQ=255)
                                                                                               cAGTGGCGGGAACAGCAACCGAAGCTGGCGCATCCGCAACTGGAGGCGCTACTGCGATGGGCCGCCATGCTGCATGAGGTCGGGTTGAATATCAACCACAGCGGTTTGCATCGCCACTCCGCTTATATTCTGCAAAACAGTGACTTGCCg                                                        <  2:4685386/150‑1 (MQ=255)
                                                                                                   ggCGGGAACAGCAACCGAAGCTGGCGCATCCGCAACTGGAGGCGCTACTGCGATGGGCCGCCATGCTGCATGAGGTCGGGTTGAATATCAACCACAGCGGTTTGCATCGCCACTCCGCTTATATTCTGCAAAACAGTGACTTGCCGGGtt                                                    <  2:1997846/150‑1 (MQ=255)
                                                                                                    gCGGGAACAGCAACCGAAGCTGGCGCATCCGCAACTGGAGGCGCTACTGCGATGGGCCGCCATGCTGCATGAGGTCGGGTTGAATATCAACCACAGCGGTTTGCATCGCCACTCCGCTTATATTCTGCAAAACAGTGACTTGCCGGGttt                                                   <  1:2493162/150‑1 (MQ=255)
                                                                                                       ggAACAGCAACCGAAGCTGGCGCATCCGCAACTGGAGGCGCTACTGCGATGGGCCGCCATGCTGCATGAGGTCGGGTTGAATATCAACCACAGCGGTTTGCATCGCCACTCCGCTTATATTCTGCAAAACAGTGACTTGCCGGGTTTTaa                                                <  1:2970244/150‑1 (MQ=255)
                                                                                                         aaCAGCAACCGAAGCTGGCGCATCCGCAACTGGAGGCGCTACTGCGATGGGCCGCCATGCTGCATGAGGTCGGGTTGAATATCAACCACAGCGGTTTGCATCGCCACTCCGCTTATATTCTGCAAAACAGTGACTTGCCGGGTTTTAATc                                              <  1:726602/150‑1 (MQ=255)
                                                                                                         aaCAGCAACCGAAGCTGGCGCATCCGCAACTGGAGGCGCTACTGCGATGGGCCGCCATGCTGCATGAGGTCGGGTTGAATATCAACCACAGCGGTTTGCATCGCCACTCCGCTTATATTCTGCAAAACAGTGACTTGCCGGGTTTTAATc                                              <  2:3696366/150‑1 (MQ=255)
                                                                                                           cAGCAACCGAAGCTGGCGCATCCGCAACTGGAGGCGCTACTGCGATGGGCCGCCATGCTGCATGAGGTCGGGTTGAATATCAACCACAGCGGTTTGCATCGCCACTCCGCTTATATTCTGCAAAACAGTGACTTGCCGGGTTTTAATCAg                                            <  1:2246029/150‑1 (MQ=255)
                                                                                                                  cGAAGCTGGCGCATCCGCAACTGGAGGCGCTACTGCGATGGGCCGCCATGCTGCATGAGGTCGGGTTGAATATCAACCACAGCGGTTTGCATCGCCACTCCGCTTATATTCTGCAAAACAGTGACTTGCCGGGTTTTAATCAGGAAcagc                                     <  2:1605549/150‑1 (MQ=255)
                                                                                                                   gAAGCTGGCGCATCCGCAACTGGAGGCGCTACTGCGATGGGCCGCCATGCTGCATGAGGTCGGGTTGAATATCAACCACAGCGGTTTGCATCGCCACTCCGCTTATATTCTGCAAAACAGTGACTTGCCGGGTTTTAATCAGGAAcagca                                    <  1:1325977/150‑1 (MQ=255)
                                                                                                                   gAAGCTGGCGCATCCGCAACTGGAGGCGCTACTGAGATGGGCCGCCATGCTGCATGAGGTCGGGTTGAATATCAACCACAGCGGTGTGCATCGCCACTCCGCTTATATTCTGCAAAACAGTGACTTGCCGGGTTTTAATCAGGAAcagca                                    <  1:1860208/150‑1 (MQ=255)
                                                                                                                       cTGGCGCATCCGCAACTGGAGGCGCTACTGCGATGGGCCGCCATGCTGCATGAGGTCGGGTTGAATATCAACCACAGCGGTTTGCATCGCCACTCCGCTTATATTCTGCAAAACAGTGACTTGCCGGGTTTTAATCAGGAACAGCAGCtg                                <  1:3085350/150‑1 (MQ=255)
                                                                                                                       cTGGCGCATCCGCAACTGGAGGCGCTACTGCGATGGGCCGCCATGCTGCATGAGGTCGGGTTGAATATCAACCACAGCGGTTTGCATCGCCACTCCGCTTATATTCTGCAAAACAGTGACTTGCCGGGTTTTAATCAGGAACAGCAGCtg                                <  1:4981156/150‑1 (MQ=255)
                                                                                                                         ggCGCATCCGCAACTGGAGGCGCTACTGCGATGGGCCGCCATGCTGCATGAGGTCGGGTTGAATATCAACCACAGCGGTTTGCATCGCCACTCCGCTTATATTCTGCAAAACAGTGACTTGCCGGGTTTTAATCAGGAACAGCAGCtgat                              <  1:2600785/150‑1 (MQ=255)
                                                                                                                         ggCGCATCCGCAACTGGAGGCGCTACTGCGATGGGCCGCCATGCTGCATGAGGTCGGGTTGAATATCAACCACAGCGGTTTGCATCGCCACTCCGCTTATATTCTGCAAAACAGTGACTTGCCGGGTTTTAATCAGGAACAGCAGCtgat                              <  2:290776/150‑1 (MQ=255)
                                                                                                                         ggCGCATCCGCAACTGGAGGCGCTACTGCGATGGGCCGCCATGCTGCATGAGGTCGGGTTGAATATCAACCACAGCGGTTTGCATCGCCACTCCGCTTATATTCTGCAAAACAGTGACTTGCCGGGTTTTAATCAGGAACAGCAGCtgat                              <  1:4528256/150‑1 (MQ=255)
                                                                                                                            gcATCCGCAACTGGAGGCGCTACTGCGATGGGCCGCCATGCTGCATGAGGTCGGGTTGAATATCAACCACAGCGGTTTGCATCGCCACTCCGCTTATATTCTGCAAAACAGTGACTTGCCGGGTTTTAATCAGGAACAGCAGCTgatgat                           >  2:3500906/1‑150 (MQ=255)
                                                                                                                            gcATCCGCAACTGGAGGCGCTACTGCGATGGGCCGCCATGCTGCATGAGGTCGGGTTGAATATCAACCACAGCGGTTTGCATCGCCACTCCGCTTATATTCTGCAAAACAGTGACTTGCCGGGTTTTAATCAGGAACAGCAGCTgatgat                           >  2:3192762/1‑150 (MQ=255)
                                                                                                                            gcATCCGCAACTGGAGGCGCTACTGCGATGGGCCGCCATGCTGCATGAGGTCGGGTTGAATATCAACCACAGCGGTTTGCATCGCCACTCCGCTTATATTCTGCAAAACAGTGACTTGCCGGGTTTTAATCAGGAACAGCAGCTgatgat                           >  2:5075288/1‑150 (MQ=255)
                                                                                                                                ccGCAACTGGAGGCGCTACTGCGATGGGCCGCCATGCTGCATGAGGTCGGGTTGAATATCAACCACAGCGGTTTGCATCGCCACTCCGCTTATATTCTGCAAAACAGTGACTTGCCGGGTTTTAATCAGGAACAGCAGCTGATGATGGCg                       <  1:3316179/150‑1 (MQ=255)
                                                                                                                                ccGCAACTGGAGGCGCTACTGCGATGGGCCGCCATGCTGCATGAGGTCGGGTTGAATATCAACCACAGCGGTTTGCATCGCCACTCCGCTTATATTCTGCAAAACAGTGACTTGCCGGGTTTTAATCAGGAACAGCAGCTGATGATGGCg                       <  2:4993938/150‑1 (MQ=255)
                                                                                                                                 cGCAACTGGAGGCGCTACTGCGATGGGCCGCCATGCTGCATGAGGTCGGGTTGAATATCAACCACAGCGGTTTGCATCGCCACTCCGCTTATATTCTGCAAAACAGTGACTTGCCGGGTTTTAATCAGGAACAGCAGCTGATGATGGCGa                      >  1:2320706/1‑150 (MQ=255)
                                                                                                                                      cTGGAGGCGCTACTGAGATGGGCCGCCATGCTGCATGAGGTCGGGTTGAATATCAACCACAGCGGTTTGCATCGCCACTCCGCTTATATTCTGCAAAACAGTGACTTGCCGGGTTTTAATCAGGAACAGCAGCTGATGATGGCGACACTg                 <  1:2721276/150‑1 (MQ=255)
                                                                                                                                       tGGAGGCGCTACTGCGATGGGCCGCCATGCTGCATGAGGTCGGGTTGAATATCAACCACAGCGGTTTGCATCGCCACTCCGCTTATATTCTGCAAAACAGTGACTTGCCGGGTTTTAATCAGGAACAGCAGCTGATGATGGCGACACTgg                <  2:2874247/150‑1 (MQ=255)
                                                                                                                                       tGGAGGCGCTACTGCGATGGGCCGCCATGCTGCATGAGGTCGGGTTGAATATCAACCACAGCGGTTTGCATCGCCACTCCGCTTATATTCTGCAAAACAGTGACTTGCCGGGTTTTAATCAGGAACAGCAGCTGATGATGGCGACACTgg                >  2:422327/1‑150 (MQ=255)
                                                                                                                                        ggAGGCGCTACTGCGATGGGCCGCCATGCTGCATGAGGTCGGGTTGAATATCAACCACAGCGGTTTGCATCGCCACTCCGCTTATATTCTGCAAAACAGTGACTTGCCGGGTTTTAATCAGGAACAGCAGCTGATGATGGCGACACTGGt               <  2:1536304/150‑1 (MQ=255)
                                                                                                                                           ggCGCTACTGAGATGGGCCGCCATGCTGCATGAGGTCGGGTTGAATATCAACCACAGCGGTTTGCATCGCCACTCCGCTTATATTCTGCAAAACAGTGACTTGCCGGGTTTTAATCAGGAACAGCAGCTGATGATGGCGACACTGGTgcg            <  2:3249874/150‑1 (MQ=255)
                                                                                                                                               cTACTGCGATGGGCCGCCATGCTGCATGAGGTCGGGTTGAATATCAACCACAGCGGTTTGCATCGCCACTCCGCTTATATTCTGCAAAACAGTGACTTGCCGGGTTTTAATCAGGAACAGCAGCTGATGATGGCGACACTGGTGCGCTAt        >  2:4835914/1‑150 (MQ=255)
                                                                                                                                                   tGCGATGGGCCGCCATGCTGCATGAGGTCGGGTTGAATATCAACCACAGCGGTTTGCATCGCCACTCCGCTTATATTCTGCAAAACAGTGACTTGCCGGGTTTTAATCAGGAACAGCAGCTGATGATGGCGACACTGGTGCGCTATCAcc    >  1:1755553/1‑150 (MQ=255)
                                                                                                                                                    gCGATGGGCCGCCATGCTGCATGAGGTCGGGTTGAATCTCAACCACAGCGGTTTGCATCGCCACTCCGCTTATATTCTGCAAAACAGTGACTTGCCGGGTTTTAATCAGGAACAGCAGCTGATGATGGCGACACTGGTGCGCTATCACCg   <  2:4486966/150‑1 (MQ=255)
                                                                                                                                                    gCGATGGGCCGCCACGCTGCATGAGGTCGGGTTGAATATCAACCACAGCGGTTTGCATCGCCACTCCGCTTATATTCTGCAAAACAGTGACTTGCCGGGTTTTAATCAGGAACAGCAGCTGATGATGGCGACACTGGTGCGCTATCACCg   <  2:3847407/150‑1 (MQ=255)
                                                                                                                                                     cGATGGGCCGCCATGCTGCATGAGGTCGGGTTGAATATCAACCACAGCGGTTTGCATCGCCACTCCGCTTATATTCTGCAAAACAGTGACTTGCCGGGTTTTAATCAGGAACAGCAGCTGATGATGGCGACACTGGTGCGCTATCACCGt  >  2:1508859/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
TGCGTAGTCGCACCGCCAGCAGCCTCGCCAACCAGTATCACATCGACAGCGAACAGGCCCGACGGGTGCTGGATACCACTATGCAAATGTACGAACAGTGGCGGGAACAGCAACCGAAGCTGGCGCATCCGCAACTGGAGGCGCTACTGCGATGGGCCGCCATGCTGCATGAGGTCGGGTTGAATATCAACCACAGCGGTTTGCATCGCCACTCCGCTTATATTCTGCAAAACAGTGACTTGCCGGGTTTTAATCAGGAACAGCAGCTGATGATGGCGACACTGGTGCGCTATCACCGT  >  CP009273/2619414‑2619712

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑