BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	2,648,521	C→A	5.0%	A399A (GCG→GCT)	pepB ←	aminopeptidase B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	2,648,521	0	C	A	5.0%	1522.0 / 31.5	378	A399A (GCG→GCT)	pepB	aminopeptidase B
Reads supporting (aligned to +/- strand): ref base C (184/174); new base A (6/14); total (190/188)
Fisher's exact test for biased strand distribution p-value = 6.95e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 381 total aligned reads displayed.

TTGAATTTGCAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATATTAGTTTACGCCGTTAACAGATTAGCTATCGTGCGCACACCAAGTCCCGTAGCGCCCGCAGACCACTGTTCAACCGGCGCTTTACGGTAAGTCGCCGAGCAGTCGATATGCAGCCAGCCTTGCTGATAGTTCTCAACAAAGTGCGACAGGAAGCCCGCCGCCGTGCTCGCGCCTGCCGGATACGCCGCGCTTCCGGTATTGTTCAGTTCGGCAAAGTTAGACGGCAGCTGGCTGCGGTGGAA  >  CP009273/2648375‑2648670

ttgAATTTGCAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATATTAGTTTACGCCGTTAACAGATTAGCTATCGTGCGCACACCAAGTCCCGTAGCGCCCGCAGACCACTGTTCAACCGGCGCTTTACGGTAAGTCGcc                                                                                                                                                    <  1:4202427/150‑1 (MQ=255)

ttgAATTTGCAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATATTAGTTTACGCCGTTAACAGATTAGCTATCGTGCGCACACCAAGTCCCGTAGCGCCCGCAGACCACTGTTCAACCGGCGCTTTACGGTAAGTCGcc                                                                                                                                                    >  1:3938040/1‑150 (MQ=255)

 tgAATTTGCAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATATTAGTTTACGCCGTTAACAGATTAGCTATCGTGCGCACACCAAGTCCCGTAGCGCCCGCAGACCACTGTTCAACCGGCGCTTTACGGTAAGTCGCCg                                                                                                                                                   <  1:3089181/150‑1 (MQ=255)

 tgAATTTGCAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATATTAGTTTACGCCGTTAACAGATTAGCTATCGTGCGCACACCAAGTCCCGTAGCGCCCGCAGACCACTGTTCAACCGGCGCTTTACGGTAAGTCGCCg                                                                                                                                                   <  2:2103672/150‑1 (MQ=255)

   aaTTTGCAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATATTAGTTTACGCCGTTAACAGATTAGCTATCGTGCGCACACCAAGTCCCGTAGCGCCCGCAGACCACTGTTCAACCGGCGCTTTACGGTAAGTCGCCGAg                                                                                                                                                 >  1:1717718/1‑150 (MQ=255)

        gCAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATATTAGTTTACGCCGTTAACAGATTAGCTATCGTGCGCACACCAAGTCCCGTAGCGCCCGCAGACCACTGTTCAACCGGCGCTTTACGGTAAGTCGCCGAGCAGTc                                                                                                                                            <  1:4679108/150‑1 (MQ=255)

        gCAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATATTAGTTTACGCCGTTAACAGATTAGCTATCGTGCGCACACCAAGTCCCGTAGCGCCCGCAGACCACTGTTCAACCGGCGCTTTACGGTAAGTCGCCGAGCAGTc                                                                                                                                            <  2:2917785/150‑1 (MQ=255)

        gCAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATATTAGTTTACGCCGTTAACAGATTAGCTATCGTGCGCACACCAAGTCCCGTAGCGCCCGCAGACCACTGTTCAACCGGCGCTTTACGGTAAGTCGCCGAGCAGTc                                                                                                                                            >  1:2314203/1‑150 (MQ=255)

           gAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATATTAGTTTACGCCGTTAACAGATTAGCTATCGTGCGCACACCAAGTCCCGTAGCGCCCGCAGACCACTGTTCAACCGGCGCTTTACGGTAAGTCGCCGAGCAGTCGat                                                                                                                                         >  2:1185904/1‑150 (MQ=255)

           gAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATATTAGTTTACGCCGTTAACAGATTAGCTATCGTGCGCACACCAAGTCCCGTAGCGCCCGCAGACCACTGTTCAACCGGCGCTTTACGGTAAGTCGCCGAGCAGTCGat                                                                                                                                         >  1:992819/1‑150 (MQ=255)

           gAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATATTAGTTTACGCCGTTAACAGATTAGCTATCGTGCGCACACCAAGTCCCGTAGCGCCCGCAGACCACTGTTCAACCGGCGCTTTACGGTAAGTCGCCGAGCAGTCGat                                                                                                                                         <  2:282017/150‑1 (MQ=255)

           gAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATATTAGTTTACGCCGTTAACAGATTAGCTATCGTGCGCACACCAAGTCCCGTAGCGCCCGCAGACCACTGTTCAACCGGCGCTTTACGGTAAGTCGCCGAGCAGTCGat                                                                                                                                         <  2:729378/150‑1 (MQ=255)

              tttGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATATTAGTTTACGCCGTTAACAGATTAGCTATCGTGCGCACACCAAGTCCCGTAGCGCCCGCAGACCACTGTTCAACCGGCGCTTTACGGTAAGTCGCCGAGCAGTCGATATg                                                                                                                                      <  1:1244524/150‑1 (MQ=255)

               ttGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATATTAGTTTACGCCGTTAACAGATTAGCTATCGTGCGCACACCAAGTCCCGTAGCGCCCGCAGACCACTGTTCAACCGGCGCTTTACGGTAAGTCGCCGAGCAGTCGATATGc                                                                                                                                     <  1:3571363/150‑1 (MQ=255)

                tGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATATTAGTTTACGCCGTTAACAGATTAGCTATCGTGCGCACACCAAGTCCCGTAGCGCCCGCAGACCACTGTTCAACCGGCGCTTTACGGTAAGTCGCCGAGCAGTCGATCTGca                                                                                                                                    >  2:2268773/1‑150 (MQ=255)

                tGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATATTAGTTTACGCCGTTAACAGATTAGCTATCGTGCGCACACCAAGTCCCGTAGCGCCCGCAGACCACTGTTCAACCGGCGCTTTACGGTAAGTCGCCGAGCAGTCGATATGca                                                                                                                                    >  1:3317036/1‑150 (MQ=255)

                tGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATATTAGTTTACGCCGTTAACAGATTAGCTATCGTGCGCACACCAAGTCCCGTAGCGCCCGCAGACCACTGTTCAACCGGCGCTTTACGGTAAGTCGCCGAGCAGTCGATATGca                                                                                                                                    >  2:3324613/1‑150 (MQ=255)

                  tAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATATTAGTTTACGCCGTTAACAGATTAGCTATCGTGCGCACACCAAGTCCCGTAGCGCCCGCAGACCACTGTTCAACCGGCGCTTTACGGTAAGTCGCCGAGCAGTCGATATGcagc                                                                                                                                  >  2:2645398/1‑150 (MQ=255)

                   aGGCCGGATAAGGCGTTTACGCCGCATCCGGCATATTAGTTTACGCCGTTAACAGATTAGCTATCGTGCGCACACCAAGTCCCGTAGCGCCCGCAGACCACTGTTCAACCGGCGCTTTACGGTAAGTAGCCGAGCAGTCGATATGcagcc                                                                                                                                 <  1:1613796/150‑1 (MQ=255)

                     gCCGGATAAGGCGTTTACGCCGCATCCGGCATATTAGTTTACGCCGTTAACAGATTAGCTATCGTGCGCACACCAAGTCCCGTAGCGCCCGCAGACCACTGTTCAACCGGCGCTTTACGGTAAGTAGCCGAGCAGTCGATATGcagccag                                                                                                                               >  1:1761895/1‑150 (MQ=255)

                        ggATAAGGCGTTTACGCCGCATCCGGCATATTAGTTTACGCCGTTAACAGATTAGCTATCGTGCGCACACCAAGTCCCGTAGCGCCCGCAGACCACTGTTCAACCGGCGCTTTACGGTAAGTAGCCGAGCAGTCGATATGCAGCCAGCCt                                                                                                                            >  1:893603/1‑150 (MQ=255)

                         gATAAGGCGTTTACGCCGCATCCGGCATATTAGTTTACGCCGTTAACAGATTAGCTATCGTGCGCACACCAAGTCCCGTAGCGCCCGCAGACCACTGTTCAACCGGCGCTTTACGGTAAGTCGCCGAGCAGTCGATATGCAGCCAGCCtt                                                                                                                           >  2:1492237/1‑150 (MQ=255)

                         gATAAGGCGTTTACGCCGCATCCGGCATATTAGTTTACGCCGTTAACAGATTAGCTATCGTGCGCACACCAAGTCCCGTAGCGCCCGCAGACCACTGTTCAACCGGCGCTTTACGGTAAGTAGCCGAGCAGTCGATATGCAGCCAGCCtt                                                                                                                           <  1:1477613/150‑1 (MQ=255)

                           tAAGGCGTTTACGCCGCATCCGGCATATTAGTTTACGCCGTTAACAGATTAGCTATCGTGCGCACACCAAGTCCCGTAGCGCCCGCAGACCACTGTTCAACCGGCGCTTTACGGTAAGTCGCCGAGCAGTCGATATGCAGCCAGCCTTGc                                                                                                                         >  1:1138732/1‑150 (MQ=255)

                            aaGGCGTTTACGCCGCATCCGGCATATTAGTTTACGCCGTTAACAGATTAGCTATCGTGCGCACACCAAGTCCCGTAGCGCCCGCAGACCACTGTTCAACCGGCGCTTTACGGTAAGTCGCCGAGCAGTCGATATGCAGCCAGCCTTGCt                                                                                                                        <  1:1055905/150‑1 (MQ=255)

                             aGGCGTTTACGCCGCATCCGGCATATTAGTTTACGCCGTTAACAGATTAGCTATCGTGCGCACACCAAGTCCCGTAGCGCCCGCAGACCACTGTTCAACCGGCGCTTTACGGTAAGTCGCCGAGCAGTCGATATGCAGCCAGCCTTGCTg                                                                                                                       <  1:3104897/150‑1 (MQ=255)

                             aGGCGTTTACGCCGCATCCGGCATATTAGTTTACGCCGTTAACAGATTAGCTATCGTGCGCACACCAAGTCCCGTAGCGCCCGCAGACCACTGTTCAACCGGCGCTTTACGGTAAGTCGCCGAGCAGTCGATATGCAGCCAGCCTTGCTg                                                                                                                       <  1:4024943/150‑1 (MQ=255)

                                cGTTTACGCCGCATCCGGCATATTAGTTTACGCCGTTAACAGATTAGCTATCGTGCGCACACCAAGTCCCGTAGCGCCCGCAGACCACTGTTCAACCGGCGCTTTACGGTAAGTCGCCGAGCAGTCGATATGCAGCCAGCCTTGCTGATa                                                                                                                    >  1:4395926/1‑150 (MQ=255)

                                 gTTTACGCCGCATCCGGCATATTAGTTTACGCCGTTAACAGATTAGCTATCGTGCGCACACCAAGTCCCGTAGCGCCCGCAGACCACTGTTCAACCGGCGCTTTACGGTAAGTCGCCGAGCAGTCGATATGCAGCCAGCCTTGCTGATAg                                                                                                                   >  1:2864938/1‑150 (MQ=255)

                                    tACGCCGCATCCGGCATATTAGTTTACGCCGTTAACAGATTAGCTATCGTGCGCACACCAAGGCCCGTAGCGCCCGCAGACCACTGTTCAACCGGCGCTTTACGGTAAGTCGCCGAGCAGTCGATATGCAGCCAGCCTTGCTGATAGTtc                                                                                                                <  1:1941106/150‑1 (MQ=255)

                                      cgccgcATCCGGCATATTAGTTTACGCCGTTAACAGATTAGCTATCGTGCGCACACCAAGTCCCGTAGCGCCCGCAGACCACTGTTCAACCGGCGCTTTACGGTAAGTCGCCGAGCAGTCGATATGCAGCCAGCCTTGCTGATAGTtctc                                                                                                              >  1:3963375/1‑150 (MQ=255)

                                       gccgcATCCGGCATATTAGTTTACGCCGTTAACAGATTAGCTATCGTGCGCACACCAAGTCCCGTAGCGCCCGCAGACCACTGTTCAACCGGCGCTTTACGGTAAGTCGCCGAGCAGTCGATATGCAGCCAGCCTTGCTGATAGTTCTca                                                                                                             >  1:4297168/1‑150 (MQ=255)

                                         cgcATCCGGCATATTAGTTTACGCCGTTAACAGATTAGCTATCGTGCGCACACCAAGTCCCGTAGCGCCCGCAGACCACTGTTCAACCGGCGCTTTACGGTAAGTCGCCGAGCAGTCGATATGCAGCCAGCCTTGCTGATAGTTCTcaac                                                                                                           <  1:4747431/150‑1 (MQ=255)

                                          gcATCCGGCATATTAGTTTACGCCGTTAACAGATTAGCTATCGTGCGCACACCAAGTCCCGTAGCGCCCGCAGACCACTGTTCAACCGGCGCTTTACGGTAAGTCGCCGAGCAGTCGATATGCAGCCAGCCTTGCTGATAGTTCTcaaca                                                                                                          <  1:953539/150‑1 (MQ=255)

                                            aTCCGGCATATTAGTTTACGCCGTTAACAGATTAGCTATCGTGCGCACACCAAGTCCCGTAGCGCCCGCAGACCACTGTTCAACCGGCGCTTTACGGTAAGTCGCCGAGCAGTCGATATGCAGCCAGCCTTGCTGATAGTTCTCAACaaa                                                                                                        >  1:750683/1‑150 (MQ=255)

                                            aTCCGGCATATTAGTTTACGCCGTTAACAGATTAGCTATCGTGCGCACACCAAGTCCCGTAGCGCCCGCAGACCACTGTTCAACCGGCGCTTTACGGTAAGTCGCCGAGCAGTCGATATGCAGCCAGCCTTGCTGATAGTTCTCAACaaa                                                                                                        >  1:867978/1‑150 (MQ=255)

                                               cGGCATATTAGTTTACGCCGTTAACAGATTAGCTATCGTGCGCACACCAAGTCCCGTAGCGCCCGCAGACCACTGTTCAACCGGCGCTTTACGGTAAGTCGCCGAGCAGTCGATATGCAGCCAGCCTTGCTGATAGTTCTCAACAAAGTg                                                                                                     <  1:3375272/150‑1 (MQ=255)

                                                 gCATATTAGTTTACGCCGTTAACAGATTAGCTATCGTGCGCACACCAAGTCCCGTAGCGCCCGCAGACCACTGTTCAACCGGCGCTTTACGGTAAGTCGCCGAGCAGTCGATATGCAGCCAGCCTTGCTGATAGTTCTCAACAAAGTGCg                                                                                                   <  1:4791176/150‑1 (MQ=255)

                                                  cATATTAGTTTACGCCGTTAACAGATTAGCTATCGTGCGCACACCAAGTCCCGTAGCGCCCGCAGACCACTGTTCAACCGGCGCTTTACGGTAAGTCGCCGAGCAGTCGATATGCAGCCAGCCTTGCTGATAGTTCTCAACCAAGTGCGa                                                                                                  >  2:4921349/1‑150 (MQ=255)

                                                     atTAGTTTACGCCGTTAACAGATTAGCTATCGTGCGCACACCAAGTCCCGTAGCGCCCGCAGACCACTGTTCAACCGGCGCTTTACGGTAAGTCGCCGAGCAGTCGATATGCAGCCAGCCTTGCTGATAGTTCTCAACAAAGTGCGACAg                                                                                               <  2:4449432/150‑1 (MQ=255)

                                                      ttAGTTTACGCCTTTAACAGATTAGCTATCGTGCGCACACCAAGTCCCGTAGCGCCCGCAGACCACTGTTCAACCGGCGCTTTACGGTAAGTCGCCGAGCAGTCGATATGCAGCCAGCCTTGCTGATAGTTCTCAACAAAGTGCGACAgg                                                                                              <  1:3194763/150‑1 (MQ=255)

                                                      ttAGTTTACGCCGTTAACAGATTAGCTATCGTGCGCACACCAAGTCCCGTAGCGCCCGCAGACCACTGTTCAACCGGCGCTTTACGGTAAGTCGCCGAGCAGTCGATATGCAGCCAGCCTTGCTGATAGTTCTCAACAAAGTGCGACAgg                                                                                              <  2:741828/150‑1 (MQ=255)

                                                        aGTTTACGCCGTTAACAGATTAGCTATCGTGCGCACACCAAGTCCCGTAGCGCCCGCAGACCACTGTTCAACCGGCGCTTTACGGTAAGTCGCCGAGCAGTCGATATGCAGCCAGCCTTGCTGATAGTTCTCAACAAAGTGCGACAGGaa                                                                                            <  2:2199266/150‑1 (MQ=255)

                                                           ttACGCCGTTAACAGATTAGCTATCGTGCGCACACCAAGTCCCGTAGCGCCCGCAGACCACTGTTCAACCGGCGCTTTACGGTAAGTCGCCGAGCAGTCGATATGCAGCCAGCCTTGCTGATAGTTCTCAACAAAGTGCGACAGGAAGcc                                                                                         <  1:500613/150‑1 (MQ=255)

                                                             aCGCCGTTAACAGATTAGCTATCGTGCGCACACCAAGTCCCGTAGCGCCCGCAGACCACTGTTCAACCGGCGCTTTACGGTAAGTCGCCGAGCAGTCGATATGCAGCCAGCCTTGCTGATAGTTCTCAACAAAGTGCGACAGGAAGCccg                                                                                       >  2:2504090/1‑150 (MQ=255)

                                                              cGCCGTTAACAGATTAGCTATCGTGCGCACACCAAGTCCCGTAGCGCCCGCAGACCACTGTTCAACCGGCGCTTTACGGTAAGTCGCCGAGCAGTCGATATGCAGCCAGCCTTGCTGATAGTTCTCAACAAAGTGCGACAGGAAGCccgc                                                                                      >  2:1363150/1‑150 (MQ=255)

                                                              cGCCGTTAACAGATTAGCTATCGTGCGCACACCAAGTCCCGTAGCGCCCGCAGACCACTGTTCAACCGGCGCTTTACGGTAAGTCGCCGAGCAGTCGATATGCAGCCAGCCTTGCTGATAGTTCTCAACAAAGTGCGACAGGAAGCccgc                                                                                      >  2:3663110/1‑150 (MQ=255)

                                                              cGCCGTTAACAGATTAGCTATCGTGCGCACACCAAGTCCCGTAGCGCCCGCAGACCACTGTTCAACCGGCGCTTTACGGTAAGTCGCCGAGCAGTCGATATGCAGCCAGCCTTGCTGATAGTTCTCAACAAAGTGCGACAGGAAGCccgc                                                                                      <  1:1010489/150‑1 (MQ=255)

                                                                 cGTTAACAGATTAGCTATCGTGCGCACACCAAGTCCCGTAGCGCCCGCAGACCACTGTTCAACCGGCGCTTTACGGTAAGTCGCCGAGCAGTCGATATGCAGCCAGCCTTGCTGATAGTTCTCAACAAAGTGCGACAGGAAGCccgccgc                                                                                   >  2:1935575/1‑150 (MQ=255)

                                                                 cGTTAACAGATTAGCTATCGTGCGCACACCAAGTCCCGTAGCGCCCGCAGACCACTGTTCAACCGGCGCTTTACGGTAAGTCGCCGAGCAGTCGATATGCAGCCAGCCTTGCTGATAGTTCTCAACAAAGTGCGACAGGAAGCccgccgc                                                                                   >  1:2487601/1‑150 (MQ=255)

                                                                  gTTAACAGATTAGCTATCGTGCGCACACCAAGTCCCGTAGCGCCCGCAGACCACTGTTCAACCGGCGCTTTACGGTAAGTCGCCGAGCAGTCGATATGCAGCCAGCCTTGCTGATAGTTCTCAACAAAGTGCGACAGGAAGCccgccgcc                                                                                  >  1:5041871/1‑150 (MQ=255)

                                                                        aGATTAGCTATCGTGCGCACACCAAGTCCCGTAGCGCCCGCAGACCACTGTTCAACCGGCGCTTTACGGTAAGTCGCCGAGCAGTCGATATGCAGCCAGCCTTGCTGATAGTTCTCAACAAAGTGCGACAGGAAGCCCGCCGCCGTGCTc                                                                            >  2:982947/1‑150 (MQ=255)

                                                                        aGATTAGCTATCGTGCGCACACCAAGTCCCGTAGCGCCCGCAGACCACTGTTCAACCGGCGCTTTACGGTAAGTCGCCGAGCAGTCGATATGCAGCCAGCCTTGCTGATAGTTCTCAACAAAGTGCGACAGGAAGCCCGCCGCCGTGCTc                                                                            <  1:843132/150‑1 (MQ=255)

                                                                        aGATTAGCTATCGTGCGCACACCAAGTCCCGTAGCGCCCGCAGACCACTGTTCAACCGGCGCTTTACGGTAAGTCGCCGAGCAGTCGATATGCAGCCAGCCTTGCTGATAGTTCTCAACAAAGTGCGACAGGAAGCCCGCCGCCGTGCTc                                                                            >  2:4359185/1‑150 (MQ=255)

                                                                        aGATTAGCTATCGTGCGCACACCAAGTCCCGTAGCGCCCGCAGACCACTGTTCAACCGGCGCTTTACGGTAAGTCGCCGAGCAGTCGATATGCAGCCAGCCTTGCTGATAGTTCTCAACAAAGTGCGACAGGAAGCCCGCCGCCGTGCTc                                                                            <  2:4622607/150‑1 (MQ=255)

                                                                         gATTAGCTATCGTGCGCACACCAAGTCCCGTAGCGCCCGCAGACCACTGTTCAACCGGCGCTTTACGGTAAGTCGCCGAGCAGTCGATATGCAGCCAGCCTTGCTGATAGTTCTCAACAAAGTGCGACAGGAAGCCCGCCGCCGTGCTcg                                                                           >  1:4846501/1‑150 (MQ=255)

                                                                                tATCGTGCGCACACCAAGTCCCGTAGCGCCCGCAGACCACTGTTCAACCGGCGCTTTACGGTAAGTCGCCGAGCAGTCGATATGCAGCCAGCCTTGCTGATAGTTCTCAACAAAGTGCGACAGGAAGCCCGCCGCCGTGCTCGCGCCTGc                                                                    >  1:618526/1‑150 (MQ=255)

                                                                                 aTCGTGCGCACACCAAGTCCCGTAGCGCCCGCAGACCACTGTTCAACCGGCGCTTTACGGTAAGTCGCCGAGCAGTCGATATGCAGCCAGCCTTGCTGATAGTTCTCAACAAAGTGCGACAGGAAGCCCGCCGCCGTGCTCGCGCCTGcc                                                                   <  2:417247/150‑1 (MQ=255)

                                                                                  tCGTGCGCACACCAAGTCCCGTAGCGCCCGCAGACCACTGTTCAACCGGCGCTTTACGGTAAGTCGCCGAGCAGTCGATATGCAGCCAGCCTTGCTGATAGTTCTCAACAAAGTGCGACAGGAAGCCCGCCGCCGTGCTCGCGCCTGCCg                                                                  <  2:3034313/150‑1 (MQ=255)

                                                                                  tCGTGCGCACACCAAGTCCCGTAGCGCCCGCAGACCACTGTTCAACCGGCGCTTTACGGTAAGTCGCCGAGCAGTCGATATGCAGCCAGCCTTGCTGATAGTTCTCAACAAAGTGCGACAGGAAGCCCGCCGCCGTGCTCGCGCCTGCCg                                                                  <  1:2431032/150‑1 (MQ=255)

                                                                                    gTGCGCACACCCAGTCCCGTAGCGCCCGCAGACCACTGTTCAACCGGCGCTTTACGGTAAGTCGCCGAGCAGTCGATATGCAGCCAGCCTTGCTGATAGTTCTCAACAAAGTGCGACAGGAAGCCCGCCGCCGTGCTCGCGCCTGCAGGa                                                                <  2:4120187/150‑1 (MQ=255)

                                                                                    gTGCGCACACCAAGTCCCGTAGCGCCCGCAGACCACTGTTCAACCGGCGCTTTACGGTAAGTCTCAGAGCAGTCGATATGCAGCCAGCCTTGCTGATAGTTCTCAACAAAGTGCGACAGGAAGCCCGCCGCCGTGCTCGCGCCTGCCGGa                                                                >  2:4691683/1‑150 (MQ=255)

                                                                                    gTGCGCACACCAAGTCCCGTAGCGCCCGCAGACCACTGTTCAACCGGCGCTTTACGGTAAGTCGCCGAGCAGTCGATATGCAGCCAGCCTTGCTGATAGTTCTCAACAAAGTGCGACAGGAAGCCCGCCGCCGTGCTCGCGCCTGCCGGa                                                                <  1:2757424/150‑1 (MQ=255)

                                                                                           cacCAAGTCCCGTAGCGCCCGCAGACCACTGTTCAACCGGCGCTTTACGGTAAGTCGCCGAGCAGTCGATATGCAGCCAGCCTTGCTGATAGTTCTCAACAAAGTGCGACAGGAAGCCCGCCGCCGTGCTCGCGCCTGCCGGATAc                                                             >  2:397902/1‑146 (MQ=255)

                                                                                            acCAAGTCCCGTAGCGCCCGCAGACCACTGTTCAACCGGCGCTTTACGGTAAGTCGCCGAGCAGTCGATATGCAGCCAGCCTTGCTGATAGTTCTCAACAAAGTGCGACAGGAAGCCCGCCGCCGTGCTCGCGCCTGCCGGATAcgccgc                                                        <  1:4065545/150‑1 (MQ=255)

                                                                                            acCAAGTCCCGTAGCGCCCGCAGACCACTGTTCAACCGGCGCTTTACGGTAAGTCGCCGAGCAGTCGATATGCAGCCAGCCTTGCTGATAGTTCTCAACAAAGTGCGACAGGAAGCCCGCCGCCGTGCTCGCGCCTGCCGGATAcgccgc                                                        <  1:1201153/150‑1 (MQ=255)

                                                                                             ccAAGTCCCGTAGCGCCCGCAGACCACTGTTCAACCGGCGCTTTACGGTAAGTCGCCGAGCAGTCGATATGCAGCCAGCCTTGCTGATAGTTCTCAACAAAGTGCGACAGGAAGCCCGCCGCCGTGCTCGCGCCTGCCGGATACGCcgcg                                                       <  1:1993323/150‑1 (MQ=255)

                                                                                              cAAGTCCCGTAGCGCCCGCAGACCACTGTTCAACCGGCGCTTTACGGTAAGTCGCCGAGCAGTCGATATGCAGCCAGCCTTGCTGATAGTTCTCAACAAAGTGCGACAGGAAGCCCGCCGCCGTGCTCGCGCCTGCCGGATACGCCgcgc                                                      >  2:2880286/1‑150 (MQ=255)

                                                                                               aaGTCCCGTAGCGCCCGCAGACCACTGTTCAACCGGCGCTTTACGGTAAGTCGCCGAGCAGTCGATATGCAGCCAGCCTTGCTGATAGTTCTCAACAAAGTGCGACAGGAAGCCCGCCGCCGTGCTCGCGCCTGCCGGATACGCCGCGCt                                                     <  1:2592503/150‑1 (MQ=255)

                                                                                                aGTCCCGTAGCGCCCGCAGACCACTGTTCAACCGGCGCTTTACGGTAAGTCGCCGAGCAGTCGATATGCAGCCAGCCTTGCTGATAGTTCTCAACAAAGTGCGACAGGAAGCCCGCCGCCGTGCTCGCGCCTGCCGGATACGCCGCGCtt                                                    <  2:66528/150‑1 (MQ=255)

                                                                                                  tCCCGTAGCGCCCGCAGACCACTGTTCAACCGGCGCTTTACGGTAAGTAGCCGAGCAGTCGATATGCAGCCAGCCTTGCTGATAGTTCTCAACAAAGTGCGACAGGAAGCCCGCCGCCGTGCTCGCGCCTGCCGGATACGCCGCGCTTcc                                                  <  2:377312/150‑1 (MQ=255)

                                                                                                   cccGTAGCGCCCGCAGACCACTGTTCAACCGGCGCTTTACGGTAAGTCGCCGAGCAGTCGATATGCAGCCAGCCTTGCTGATAGTTCTCAACAAAGTGCGACAGGAAGCCCGCCGCCGTGCTCGCGCCTGCCGGATACGCCGCGCTTCCg                                                 <  1:4597677/150‑1 (MQ=255)

                                                                                                   cccGTAGCGCCCGCAGACCACTGTTCAACCGGCGCTTTACGGTAAGTCGCCGAGCAGTCGATATGCAGCCAGCCTTGCTGATAGTTCTCAACAAAGTGCGACAGGAAGCCCGCCGCCGTGCTCGCGCCTGCCGGATACGCCGCGCTTCCg                                                 >  2:5256/1‑150 (MQ=255)

                                                                                                   cccGTAGCGCCCGCAGACCACTGTTCAACCGGCGCTTTACGGTAAGTCGCCGAGCAGTCGATATGCAGCCAGCCTTGCTGATAGTTCTCAACAAAGTGCGACAGGAAGCCCGCCGCCGTGCTCGCGCCTGCCGGATACGCCGCGCTTCCg                                                 <  2:843450/150‑1 (MQ=255)

                                                                                                      gTAGCGCCCGCAGACCACTGTTCAACCGGCGCTTTACGGTAAGTCGCCGAGCAGTCGATATGCAGCCAGCCTTGCTGATAGTTCTCAACAAAGTGCGACAGGAAGCCCGCCGCCGTGCTCGCGCCTGCCGGATACGCCGCGCTTCCGGTa                                              >  2:4052015/1‑150 (MQ=255)

                                                                                                      gTAGCGCCCGCAGACCACTGTTCAACCGGCGCTTTACGGTAAGTCGCCGAGCAGTCGATATGCAGCCAGCCTTGCTGATAGTTCTCAACAAAGTGCGACAGGAAGCCCGCCGCCGTGCTCGCGCCTGCCGGATACGCCGCGCTTCCGGTa                                              <  2:3900561/150‑1 (MQ=255)

                                                                                                          cgcCCGCAGACCACTGTTCAACCGGCGCTTTACGGTAAGTCGCCGAGCAGTCGATATGCAGCCAGCCTTGCTGATAGTTCTCAACAAAGTGCGACAGGAAGCCCGCCGCCGTGCTCGCGCCTGCCGGATACGCCGCGCTTCCGGTATTGt                                          >  2:2397962/1‑150 (MQ=255)

                                                                                                           gcCCGCAGACCACTGTTCAACCGGCGCTTTACGGTAAGTCGCCGAGCAGTCGATATGCAGCCAGCCTTGCTGATAGTTCTCAACAAAGTGCGACAGGAAGCCCGCCGCCGTGCTCGCGCCTGCCGGATACGCCGCGCTTCCGGTATTGtt                                         >  2:3295998/1‑150 (MQ=255)

                                                                                                           gcCCGCAGACCACTGTTCAACCGGCGCTTTACGGTAAGTCGCCGAGCAGTCGATATGCAGCCAGCCTTGCTGATAGTTCTCAACAAAGTGCGACAGGAAGCCCGCCGCCGTGCTCGCGCCTGCCGGATACGCCGCGCTTCCGGTATTGtt                                         >  1:2953180/1‑150 (MQ=255)

                                                                                                           gcCCGCAGACCACTGTTCAACCGGCGCTTTACGGTAAGTCGCCGAGCAGTCGATATGCAGCCAGCCTTGCTGATAGTTCTCAACAAAGTGCGACAGGAAGCCCGCCGCCGTGCTCGCGCCTGCCGGATACGCCGCGCTTCCGGTATTGtt                                         <  1:2670622/150‑1 (MQ=255)

                                                                                                             ccGCAGACCACTGTTCAACCGGCGCTTTACGGTAAGTCGCCGAGCAGTCGATATGCAGCCAGCCTTGCTGATAGTTCTCAACAAAGTGCGACAGGAAGCCCGCCGCCGTGCTCGCGCCTGCCGGATACGCCGCGCTTCCGGTATTGTTCa                                       >  2:3819761/1‑150 (MQ=255)

                                                                                                             ccGCAGACCACTGTTCAACCGGCGCTTTACGGTAAGTCGCCGAGCAGTCGATATGCAGCCAGCCTTGCTGATAGTTCTCAACAAAGTGCGACAGGAAGCCCGCCGCCGTGCTCGCGCCTGCCGGATACGCCGCGCTTCCGGTATTGTTCa                                       <  2:1257238/150‑1 (MQ=255)

                                                                                                               gCAGACCACTGTTCAACCGGCGCTTTACGGTAAGTCGCCGAGCAGTCGATATGCAGCCAGCCTTGCTGATAGTTCTCAACAAAGTGCGACAGGAAGCCCGCCGCCGTGCTCGCGCCTGCCGGATACGCCGCGCTTCCGGTATTGTTCAGt                                     <  1:455617/150‑1 (MQ=255)

                                                                                                                cAGACCACTGTTCAACCGGCGCTTTACGGTAAGTCGCCGAGCAGTCGATATGCAGCCAGCCTTGCTGATAGTTCTCAACAAAGTGCGACAGGAAGCCCGCCGCCGTGCTCGCGCCTGCCGGATACGCCGCGCTTCCGGTATTGTTCAGtt                                    <  2:4830674/150‑1 (MQ=255)

                                                                                                                 aGACCACTGTTCAACCGGCGCTTTACGGTAAGTCGCCGAGCAGTCGATATGCAGCCAGCCTTGCTGATAGTTCTCAACAAAGTGCGACAGGAAGCCCGCCGCCGTGCTCGCGCCTGCCGGATACGCCGCGCTTCCGGTATTGTTCAGTTc                                   >  1:4889113/1‑150 (MQ=255)

                                                                                                                   aCCACTGTTCAACCGGCGCTTTACGGTAAGTCGCCGAGCAGTCGATATGCAGCCAGCCTTGCTGATAGTTCTCAACAAAGTGCGACAGGAAGCCCGCCGCCGTGCTCGCGCCTGCCGGATACGCCGCGCTTCCGGTATTGTTCAGTTCgg                                 <  1:2976089/150‑1 (MQ=255)

                                                                                                                     cACTGTTCAACCGGCGCTTTACGGTAAGTAGCCGAGCAGTCGATATGCAGCCAGCCTTGCTGATAGTTCTCAACAAAGTGCGACAGGAAGCCCGCCGCCGTGCTCGCGCCTGCCGGATACGCCGCGCTTCCGGTATTGTTCAGTTCGGCa                               <  2:1202070/150‑1 (MQ=255)

                                                                                                                      aCTGTTCAACCGGCGCTTTACGGTAAGTCGCCGAGCAGTCGATATGCAGCCAGCCTTGCTGATAGTTCTCAACAAAGTGCGACAGGAAGCCCGCCGCCGTGCTCGCGCCTGCCGGATACGCCGCGCTTCCGGTATTGTTCAGTTCGGCaa                              <  2:3461811/150‑1 (MQ=255)

                                                                                                                       cTGTTCAACCGGCGCTTTACGGTAAGTCGCCGAGCAGTCGATATGCAGCCAGCCTTGCTGATAGTTCTCAACAAAGTGCGACAGGAAGCCCGCCGCCGTGCTCGCGCCTGCCGGATACGCCGCGCTTCCGGTATTGTTCAGTTCGGCaaa                             <  1:5077703/150‑1 (MQ=255)

                                                                                                                         gTTCAACCGGCGCTTTACGGTAAGTCGCCGAGCAGTCGATATGCAGCCAGCCTTGCTGATAGTTCTCAACAAAGTGCGACAGGAAGCCCGCCGCCGTGCTCGCGCCTGCCGGATACGCCGCGCTTCCGGTATTGTTCAGTTCGGCAAAGt                           <  1:4618960/150‑1 (MQ=255)

                                                                                                                                  gcgcTTTACGGTAAGTCGCCGAGCAGTCGATATGCAGCCAGCCTTGCTGATAGTTCTCAACAAAGTGCGACAGGAAGCCCGCCGCCGTGCTCGCGCCTGCCGGATACGCCGCGCTTCCGGTATTGTTCAGTTCGGCAAAGTTAGACGGCa                  <  2:2010628/150‑1 (MQ=255)

                                                                                                                                   cgcTTTACGGTAAGTCGCCGAGCAGTCGATATGCAGCCAGCCTTGCTGATAGTTCTCAACAAAGTGCGACAGGAAGCCCGCCGCCGTGCTCGCGCCTGCCGGATACGCCGCGCTTCCGGTATTGTTCAGTTCGGCAAAGTTAGACGGCAg                 <  1:1414098/150‑1 (MQ=255)

                                                                                                                                    gcTTTACGGTAAGTCGCCGAGCAGTCGATATGCAGCCAGCCTTGCTGATAGTTCTCAACAAAGTGCGACAGGAAGCCCGCCGCCGTGCTCGCGCCTGCCGGATACGCCGCGCTTCCGGTATTGTTCAGTTCGGCAAAGTTAGACGGCAgc                <  2:2151046/150‑1 (MQ=255)

                                                                                                                                     cTTTACGGTAAGTCGCCGAGCAGTCGATATGCAGCCAGCCTTGCTGATAGTTCTCAACAAAGTGCGACAGGAAGCCCGCCGCCGTGCTCGCGCCTGCCGGATACGCCGCGCTTCCGGTATTGTTCAGTTCGGCAAAGTTAGACGGCAgct               <  1:3890136/150‑1 (MQ=255)

                                                                                                                                       ttACGGTAAGTCGCCGAGCAGTCGATATGCAGCCAGCCTTGCTGATAGTTCTCAACAAAGTGCGACAGGAAGCCCGCCGCCGTGCTCGCGCCTGCCGGATACGCCGCGCTTCCGGTATTGTTCAGTTCGGCAAAGTTAGACGGCAgctgg             >  2:1823115/1‑150 (MQ=255)

                                                                                                                                           ggTAAGTCGCCGAGCAGTCGATATGCAGCCAGCCTTGCTGATAGTTCTCAACAAAGTGCGACAGGAAGCCCGCCGCCGTGCTCGCGCCTGCCGGATACGCCGCGCTTCCGGTATTGTTCAGTTCGGCAAAGTTAGACGGCAGCTGGCTGc         >  2:2563449/1‑150 (MQ=255)

                                                                                                                                            gTAAGTCGCCGAGCAGTCGATATGCAGCCAGCCTTGCTGATAGTTCTCAACAAAGTGCGACAGGAAGCCCGCCGCCGTGCTCGCGCCTGCCGGATACGCCGCGCTTCCGGTATTGTTCAGTTCGGCAAAGTTAGACGGCAGCTGGCTGCg        >  1:3727951/1‑150 (MQ=255)

                                                                                                                                            gTAAGTCGCCGAGCAGTCGATATGCAGCCAGCCTTGCTGATAGTTCTCAACAAAGTGCGACAGGAAGCCCGCCGCCGTGCTCGCGCCTGCCGGATACGACTCGCTTCAGGTATTGTTCAGTTCGGCAAAGTTAGACGGCAGCTGGCTGCg        >  1:1570178/1‑150 (MQ=255)

                                                                                                                                               aGTCGCCGAGCAGTCGATATGCAGCCAGCCTTGCTGATAGTTCTCAACAAAGTGCGACAGGAAGCCCGCCGCCGTGCTCGCGCCTGCCGGATACGCCGCGCTTCCGGTATTGTTCAGTTCGGCAAAGTTAGACGGCAGCTGGCTGCGGTg     >  2:16520/1‑150 (MQ=255)

                                                                                                                                                  cGCCGAGCAGTCGATATGCAGCCAGCCTTGCTGATAGTTCTCAACAAAGTGCGACAGGAAGCCCGCCGCCGTGCTCGCGCCTGCCGGATACGCCGCGCTTCCGGTATTGTTCAGTTCGGCAAAGTTAGACGGCAGCTGGCTGCGGTGGaa  <  1:2178738/150‑1 (MQ=255)

TTGAATTTGCAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATATTAGTTTACGCCGTTAACAGATTAGCTATCGTGCGCACACCAAGTCCCGTAGCGCCCGCAGACCACTGTTCAACCGGCGCTTTACGGTAAGTCGCCGAGCAGTCGATATGCAGCCAGCCTTGCTGATAGTTCTCAACAAAGTGCGACAGGAAGCCCGCCGCCGTGCTCGCGCCTGCCGGATACGCCGCGCTTCCGGTATTGTTCAGTTCGGCAAAGTTAGACGGCAGCTGGCTGCGGTGGAA  >  CP009273/2648375‑2648670

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`