BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	2,811,275	T→G	6.9%	intergenic (‑56/+143)	argQ ← / ← argZ	tRNA‑Arg/tRNA‑Arg

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	2,811,275	0	T	G	6.9%	742.5 / 44.3	259	intergenic (‑56/+143)	argQ/argZ	tRNA‑Arg/tRNA‑Arg
Reads supporting (aligned to +/- strand): ref base T (96/144); new base G (11/7); total (107/152)
Fisher's exact test for biased strand distribution p-value = 8.81e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.17e-01

Only 100 of 264 total aligned reads displayed.

GAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCA  >  CP009273/2811126‑2811424

gAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCTGTTCGTAGCCGAGTACTCTTTCCAGCTGAGCTACGGATGCATCGTGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAAt                                                                                                                                                       <  1:5143855/150‑1 (MQ=255)

  aGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTa                                                                                                                                                     <  2:2029493/150‑1 (MQ=255)

   ggTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTaa                                                                                                                                                    <  2:641635/150‑1 (MQ=255)

      tACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAagag                                                                                                                                                 <  2:782956/150‑1 (MQ=255)

       aCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGA                                                                                                                                                >  2:4621959‑M1/1‑150 (MQ=255)

          gagaATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGG                                                                                                                                             <  2:2691543‑M1/150‑1 (MQ=255)

           agaATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGT                                                                                                                                            <  2:4369215‑M1/150‑1 (MQ=255)

             aaTATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGC                                                                                                                                          <  1:2398981‑M1/150‑1 (MQ=255)

             aaTATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGC                                                                                                                                          <  1:2138193‑M1/150‑1 (MQ=255)

                ctGGTGCACCCGGGAGGATTCGACCCTCCCACAGATCGTTTCCCATCAGTGCACTCTTCCGATCTTTGCAAGGTAGGC‑TAGGGTAAAATTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATcc                                                                                                                                      <  2:2485207/149‑1 (MQ=2)

                         ccGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGAT                                                                                                                              <  1:1524863‑M1/150‑1 (MQ=255)

                          cGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATT                                                                                                                             <  1:3096144‑M1/150‑1 (MQ=255)

                            ggaggaTTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTAc                                                                                                                           <  2:1570599/150‑1 (MQ=255)

                            ggaggaTTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTAc                                                                                                                           <  2:2602908/150‑1 (MQ=255)

                            ggaggaTTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTAc                                                                                                                           >  2:1689131/1‑150 (MQ=255)

                                    cgcaCCTCCGACCGCTCGGTTCGTAGCCGAGAACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTg                                                                                                                   <  2:3027828/147‑1 (MQ=255)

                                    cGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTg                                                                                                                   <  2:3027517/150‑1 (MQ=255)

                                      aaCCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCATCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGATATGGTGCATCCGGGAGGATTACTCGCCTTGCg                                                                                                                 <  2:4500889/150‑1 (MQ=255)

                                      aaCCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCg                                                                                                                 <  1:1357981/150‑1 (MQ=255)

                                       aCCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTCCTGAGGATATTGATACCGCTACGATAGCCATAGCAATGAAGAGTTGGTGCATCCGGGAGGATTACTCGCCTTGCgg                                                                                                                >  2:4593514/1‑150 (MQ=17)

                                           ccGACCGCTCGGTTCGTAGCCGAGTACTCTAGCCAGCTGAGCTACGGATGCATCGGGAAAACATATACTACTGCAGAGATTGATACCGCGACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCg                                                                                                            <  2:3806676/150‑1 (MQ=17)

                                               ccGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCt                                                                                                        <  1:665898/150‑1 (MQ=255)

                                                 gCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTc                                                                                                      <  1:3460456/150‑1 (MQ=255)

                                                  cTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGAATACTCGCCTTGAGGCTCGCCGTTCg                                                                                                     >  2:3304481/1‑150 (MQ=255)

                                                  cTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCAAATAAAGCCATATCAATTAAGAGATGGTGCAACCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCg                                                                                                     >  1:1522046/1‑150 (MQ=255)

                                                     ggTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGc                                                                                                  >  1:1781125/1‑150 (MQ=255)

                                                     ggTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGc                                                                                                  <  2:4035750/150‑1 (MQ=255)

                                                       ttCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCg                                                                                                <  2:3362203/150‑1 (MQ=255)

                                                         cGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGtt                                                                                              >  1:4415678/1‑150 (MQ=255)

                                                           tAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGc                                                                                            <  1:3501888/150‑1 (MQ=255)

                                                              ccGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTaa                                                                                         <  1:1017414/150‑1 (MQ=255)

                                                              ccGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTaa                                                                                         <  2:1501221/150‑1 (MQ=255)

                                                               cGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATCTTATTCTACTGCTGAAATTGAAACCGCTACTAAAGACATAACAATTAAGACAATGTGCATCCGGGAGGATTACTCGCCTGGCGGCTCGCCCGTCGGGCCGTGGCTaaa                                                                                        >  1:5099880/1‑150 (MQ=17)

                                                               cGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTaaa                                                                                        >  1:4775194/1‑150 (MQ=255)

                                                                gAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAg                                                                                       <  2:4222093/150‑1 (MQ=255)

                                                                  gTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCa                                                                                     <  2:2182051/150‑1 (MQ=255)

                                                                    aCTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAAc                                                                                   <  2:4954377/150‑1 (MQ=255)

                                                                     ctctATCCAGCTGAGCTACGGATGCATCGGGAAAAGTAATTATAATGATGATATTTATACCGCAACTAAAGCCATTTCAATTAAGATATGGTGCATCCGGGAGGATTACTAGCAATGAGGCTCGCCGTACTGGCCGTTGCGAAAGCaagg                                                                                  >  2:2172472/1‑148 (MQ=17)

                                                                     ctctATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACg                                                                                  >  1:4390873/1‑150 (MQ=255)

                                                                      tctATCCAGCTGAGCTACGGATGCATCGGGAAAACTAATACTACTGCTGATATTGAAACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGGTGCTACAGCAACGt                                                                                 >  1:3037633/1‑150 (MQ=17)

                                                                       ctATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGtt                                                                                >  2:3816988/1‑150 (MQ=255)

                                                                       ctATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGtt                                                                                <  1:2050579/150‑1 (MQ=255)

                                                                        tATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTa                                                                               <  1:2860499/150‑1 (MQ=255)

                                                                        tATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCGTGCGGCTCGCCATTCGGGCCGTTGCTAAAGCAACGtaa                                                                               >  1:261932/1‑148 (MQ=255)

                                                                        gaTCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTTTGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTa                                                                               <  1:4127336/149‑1 (MQ=255)

                                                                         aTCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGc                                                                                                  <  2:1853013/130‑1 (MQ=255)

                                                                         aTCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGc                                                                                                  >  1:1853013/1‑130 (MQ=255)

                                                                         aTCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTat                                                                              <  2:4333379/150‑1 (MQ=255)

                                                                            ccgCTGCGCTACGGTTGCATCGGGCATATTTATTCTACTGCTGTTATAGATAGCGCTTCTATAGCCATATCAATTACTAGATTGTGAATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTatcat                                                                           <  1:2764728/148‑1 (MQ=17)

                                                                                tGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCt                                                                       >  2:2714930/1‑150 (MQ=255)

                                                                                tGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCt                                                                       <  1:3017284/150‑1 (MQ=255)

                                                                                 gAGCTACGGATGCATCGGGAAATCTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCTCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTa                                                                      <  1:3300371/150‑1 (MQ=255)

                                                                                   gcgaCGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTTAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTTCTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGt                                                                    <  2:4091190/147‑1 (MQ=255)

                                                                                       cGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCGTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCt                                                                >  1:2499852/1‑150 (MQ=255)

                                                                                         gATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGc                                                              >  1:1068446/1‑150 (MQ=255)

                                                                                              aTCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTa                                                         <  2:1653488/150‑1 (MQ=255)

                                                                                                 gggAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTcgaacctccgaccgctcggttcgtagccgagtactctatccagctgagctacggatgcatcgggaa                                                           <  2:1019285‑M1/145‑67 (MQ=255)

                                                                                                  ggAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTc                                                     >  1:2596764/1‑150 (MQ=255)

                                                                                                   gAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTcc                                                    >  1:812221/1‑150 (MQ=255)

                                                                                                    aaaaCTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCg                                                   >  2:2620626/1‑150 (MQ=255)

                                                                                                    aaaaCTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTAGCGGCTCGCCCTACGGGCCGTTGCTAAAGCCACGTTATCATCCCTAGTGCCTGCGGTTAATTCCg                                                   >  2:698831/1‑150 (MQ=255)

                                                                                                      aaCTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCt                                                 >  2:3508738/1‑150 (MQ=255)

                                                                                                      aaCTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCt                                                 <  2:903921/150‑1 (MQ=255)

                                                                                                      aaCTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACACGCAATGCGGCTCGCCCAGCGGGCCGTTGCTATAGCGACGTTATCATCCCTAGTGCCTGCGGTGAATTCCGCt                                                 >  2:1281084/1‑150 (MQ=18)

                                                                                                       aCTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTa                                                >  1:3661368/1‑150 (MQ=255)

                                                                                                          tcttCTACTGCTGATATTGATACCTCTAATACAGCCATATCAATTATGATATGGTGCTTCCGGGTGGATTTCTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACct                                             <  2:2639643/148‑1 (MQ=18)

                                                                                                           aTTTTACTGCTGATATTGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTcgaacctccgaccgctcggttcgtagccgagtactctatccagctgagctacggatgcaaatggaggagaggcggggagtc                                            >  2:4674989‑M1/1‑69 (MQ=255)

                                                                                                            ttcttcTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACctct                                           <  1:3207009/150‑1 (MQ=255)

                                                                                                             tcttctGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCtt                                          <  1:3378760/150‑1 (MQ=255)

                                                                                                              ctcctgctgATATTGATACCGCTACTAACGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTc                                         <  2:1119639/147‑1 (MQ=255)

                                                                                                              cgaCTGCTGATATTGATACCGCTACTAAAGCCATATCAATTATGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTc                                         <  2:4725290/148‑1 (MQ=255)

                                                                                                              cTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTTAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTc                                         <  1:3583387/150‑1 (MQ=255)

                                                                                                                  tgctgATATTGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTcgaacctccgaccgctcggttcgtagccgagtactctatccagctgagctacggatgcaaatggcggtgaggcggggattcgaacccc                                     >  2:1894394‑M1/1‑62 (MQ=255)

                                                                                                                     tgATATTGATACCGCTACTACAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTCAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCt                                  <  2:4960377/150‑1 (MQ=255)

                                                                                                                        tatTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTAGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCa                               >  1:2737463/1‑150 (MQ=255)

                                                                                                                         atTGATACCGCTACTAAAGCCTTATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTc                                                                                                             <  1:1244042/71‑1 (MQ=25)

                                                                                                                         atTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTc                                                                                                             >  2:1244042/1‑71 (MQ=40)

                                                                                                                         atTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCAt                              <  1:3709217/150‑1 (MQ=255)

                                                                                                                         atTGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTcgaacctccgaccgctcggttcgtagccgagtactctatccagctgagctacggatgcaaatggcggtgaggcggggattcgaaccccggatgca                              >  2:4830888‑M1/1‑55 (MQ=255)

                                                                                                                            gATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCAAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCCTTGc                           >  1:1636984/1‑150 (MQ=255)

                                                                                                                            gATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAG                                                                                                                                 >  1:4562897‑M1/1‑48 (MQ=255)

                                                                                                                               agatcttctaACGCCATATTTTTTCCGAGATGGTGCCTCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGa                        <  1:4142360/143‑1 (MQ=14)

                                                                                                                                ccGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGaa                       >  1:4027273/1‑150 (MQ=255)

                                                                                                                                ccGCTACTAAAGCCATATCAATTAAGAGATGGGGCCTCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGaa                       <  1:3938943/150‑1 (MQ=255)

                                                                                                                                 cGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAg                      >  1:1291947/1‑150 (MQ=255)

                                                                                                                                   ctactaATCCGATATCAAGTATGAGATGGTGCATCCGGGAGGATTcgaacctccgaccgctcggttcgtagccgagtactctatccagctgagctacggatgcaaatggcggtgaggcggggattcgaaccccggatgcagcttttgacc                    <  1:4674989‑M1/150‑106 (MQ=255)

                                                                                                                                     actaAAGCCATATCACTTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGAttt                  <  2:3108461/150‑1 (MQ=255)

                                                                                                                                     actaAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGAttt                  <  2:3420869/150‑1 (MQ=255)

                                                                                                                                      ctaAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTc                 <  1:2312097/150‑1 (MQ=255)

                                                                                                                                      ctaAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTcgaacctccgaccgctcggttcgtagccgagtactctatccagctgagctacggatgcaaatggcggtgaggcggggat                                              >  1:4835378‑M1/1‑42 (MQ=255)

                                                                                                                                        aaaGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTcgaacctccgaccgctcggttcgtagccgagtactctatccagctgagctacggatgcaaatggcggt                                                         >  2:3704357‑M1/1‑40 (MQ=255)

                                                                                                                                         aaGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTatcctc                                                                          >  1:2364277/1‑90 (MQ=18)

                                                                                                                                              atatCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAata         <  1:100478/150‑1 (MQ=255)

                                                                                                                                               tatCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAatat        <  1:1689131/150‑1 (MQ=255)

                                                                                                                                                 tCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATgg      >  1:2648636/1‑150 (MQ=255)

                                                                                                                                                  cAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGt     <  1:4621959/150‑1 (MQ=255)

                                                                                                                                                  cAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGt     >  1:1082880/1‑150 (MQ=255)

                                                                                                                                                  cAATTAAGAGATGGTGAATCAGGTATGATTACTCGCCTTGCTGCTAGCCCTTATGTCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGt     <  2:3912939/150‑1 (MQ=18)

                                                                                                                                                    aTTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGc   >  2:3570293/1‑150 (MQ=255)

                                                                                                                                                    aTTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTAGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGAGCCTGAGGTTAATTCAGCTACCACTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGc   >  1:842096/1‑150 (MQ=18)

GAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCA  >  CP009273/2811126‑2811424

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`