Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,888,584 G→T 94.1% I185I (ATC→ATA)  ygcQ ← putative flavoprotein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,888,5840GT94.1% 1030.2 / 50.5 320I185I (ATC→ATA) ygcQputative flavoprotein
Reads supporting (aligned to +/- strand):  ref base G (8/11);  new base T (149/152);  total (157/163)
Fisher's exact test for biased strand distribution p-value = 6.38e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.26e-01

Only 100 of 320 total aligned reads displayed.

TGCGCCAGAAGCGCCAACAACAATACACACCTCAGGTGCCAGCAGATGTCCGGAAATACCAATCACTTTTTCAGCATCAACGCCACCGTTCATCACTCGCGCCCGACTGTAGCCCACTTCGGCTCCCAGCTTTTCTGCCAGCATAGCGATTTCCTGGTTATCTGCCTCTCCGCCCTGCCCCACCACCAGCACCCGTCTGGCCTCAGCTAATGGGTCGCGGGTAACATTTTTCAGATCCTCTGTGCTAACCAGCCAGTCCGGAAGCGCACCCGGGACAATGTTCAGTTGTTGCAT  >  CP009273/2888437‑2888730
                                                                                                                                                   |                                                                                                                                                  
 gcgcCAGAAGCGCCAACAACAATACACACCTCAGGTGCCAGCAGATGTCCGGAAATACCAATCACTTTTTCAGCATCAACGCCACCGTTCATCACTCGCGCCCGACTGTAGCCCACTTCGGCTCCCAGCTTTTCTGCCAGCATAGCTAtt                                                                                                                                                 <  2:1727972/150‑1 (MQ=255)
 gcgcCAGAAGCGCCAACAACAATACACACCTCAGGTGCCAGCAGATGTCCGGAAATACCAATCACTTTTTCAGCATCAACGCCACCGTTCATCACTCGCGCCCGACTGTAGCCCACTTCGGCTCCCAGCTTTTCTGCCAGCATAGCTAtt                                                                                                                                                 <  1:2217757/150‑1 (MQ=255)
 gcgcCAGAAGCGCCAACAACAATACACACCTCAGGTGCCAGCAGATGTCCGGAAATACCAATCACTTTTTCAGCATCAACGCCACCGTTCATCACTCGCGCCCGACTGTAGCCCACTTCGGCTCCCAGCTTTTCTGCCAGCATAGCTAtt                                                                                                                                                 <  2:2520296/150‑1 (MQ=255)
  cgcCAGAAGCGCCAACAACAATACACACCTCAGGTGCCAGCAGATGTCCGGAAATACCAATCACTTTTTCAGCATCAACGCCACCGTTCATCACTCGCGCCCGACTGTAGCCCACTTCGGCTCCCAGCTTTTCTGCCAGCATAGCTAttt                                                                                                                                                >  2:4386677/1‑150 (MQ=255)
   gcCAGAAGCGCCAACAACAATACACACCTCAGGTGCCAGCAGATGTCCGGAAATACCAATCACTTTTTCAGCATCAACGCCACCGTTCATCACTCGCGCCCGACTGTAGCCCACTTCGGCTCCCAGCTTTTCTGCCAGCATAGCTATTTc                                                                                                                                               >  1:3960193/1‑150 (MQ=255)
    ccAGAAGCGCCAACAACAATACACACCTCAGGTGCCAGCAGATGTCCGGAAATACCAATCACTTTTTCAGCATCAACGCCACCGTTCATCACTCGCGCCCGACTGTAGCCCACTTCGGCTCCCAGCTTTTCTGCCAGCATAGCTATTTcc                                                                                                                                              <  2:4015952/150‑1 (MQ=255)
     cAGAAGCGCCAACAACAATACACACCTCAGGTGCCAGCAGATGTCCGGAAATACCAATCACTTTTTCAGCATCAACGCCACCGTTCATCACTCGCGCCCGACTGTAGCCCACTTCGGCTCCCAGCTTTTCTGCCAGCATAGCTATTTCCt                                                                                                                                             <  1:1635980/150‑1 (MQ=255)
         aGCGCCAACAACAATACACACCTCAGGTGCCAGCAGATGTCCGGAAATACCAATCACTTTTTCAGCATCAACGCCACCGTTCATCACTCGCGCCCGACTGTAGCCCACTTCGGCTCCCAGCTTTTCTGCCAGCATAGCTATTTCCTGGtt                                                                                                                                         >  1:274711/1‑150 (MQ=255)
         aGCGCCAACAACAATACACACCTCAGGTGCCAGCAGATGTCCGGAAATACCAATCACTTTTTCAGCATCAACGCCACCGTTCATCACTCGCGCCCGACTGTAGCCCACTTCGGCTCCCAGCTTTTCTGCCAGCATAGCTATTTCCTGGtt                                                                                                                                         >  1:4475983/1‑150 (MQ=255)
         aGCGCCAACAACAATACACACCTCAGGTGCCAGCAGATGTCCGGAAATACCAATCACTTTTTCAGCATCAACGCCACCGTTCATCACTCGCGCCCGACTGTAGCCCACTTCGGCTCCCAGCTTTTCTGCCAGCATAGCTATTTCCTGGtt                                                                                                                                         <  1:4160359/150‑1 (MQ=255)
           cgcCAACAACAATACACACCTCAGGTGCCAGCAGATGTCCGGAAATACCAATCACTTTTTCAGCATCAACGCCACCGTTCATCACTCGCGCCCGACTGTAGCCCACTTCGGCTCCCAGCTTTTCTGCCAGCATAGCTATTTCCTGGTTAt                                                                                                                                       <  1:2775853/150‑1 (MQ=255)
           cgcCAACAACAATACACACCTCAGGTGCCAGCAGATGTCCGGAAATACCAATCACTTTTTCAGCATCAACGCCACCGTTCATCACTCGCGCCCGACTGTAGCCCACTTCGGCTCCCAGCTTTTCTGCCAGCATAGCTATTTCCTGGTTAt                                                                                                                                       <  2:1195464/150‑1 (MQ=255)
             ccAACAACAATACACACCTCAGGTGCCAGCAGATGTCCGGAAATACCAATCACTTTTTCAGCATCAACGCCACCGTTCATCACTCGCGCCCGACTGTAGCCCACTTCGGCTCCCAGCTTTTCTGCCAGCATAGCTATTTCCTGGTTATCt                                                                                                                                     <  1:666227/150‑1 (MQ=255)
              caacaacaaTACACACCTCAGGTGCCAGCAGATGTCCGGAAATACCAATCACTTTTTCAGCATCAACGCCACCGTTCATCACTCGCGCCCGACTGTAGCCCACTTCGGCTCCCAGCTTTTCTGCCAGCATAGCTATTTCCTGGTTATCTg                                                                                                                                    <  2:2655117/150‑1 (MQ=255)
                acaacaaTACACACCTCAGGTGCCAGCAGATGTCCGGAAATACCAATCACTTTTTCAGCATCAACGCCACCGTTCATCACTCGCGCCCGACTGTAGCCCACTTCGGCTCCCAGCTTTTCTGCCAGCATAGCTATTTCCTGGTTATCTGcc                                                                                                                                  >  2:1046142/1‑150 (MQ=255)
                acaacaaTACACACCTCAGGTGCCAGCAGATGTCCGGAAATACCAATCACTTTTTCAGCATCAACGCCACCGTTCATCACTCGCGCCCGACTGTAGCCCACTTCGGCTCCCAGCTTTTCTGCCAGCATAGCTATTTCCTGGTTATCTGcc                                                                                                                                  >  1:4761305/1‑150 (MQ=255)
                  aacaaTACACACCTCAGGTGCCAGCAGATGTCCGGAAATACCAATCACTTTTTCAGCATCAACGCCACCGTTCATCACTCGCGCCCGACTGTAGCCCACTTCGGCTCCCAGCTTTTCTGCCAGCATAGCTATTTCCTGGTTATCTGCctc                                                                                                                                >  1:1485554/1‑150 (MQ=255)
                    caaTACACACCTCAGGTGCCAGCAGATGTCCGGAAATACCAATCACTTTTTCAGCATCAACGCCACCGTTCATCACTCGCGCCCGACTGTAGCCCACTTCGGCTCCCAGCTTTTCTGCCAGCATAGCTATTTCCTGGTTATCTGCCtctc                                                                                                                              >  2:4617184/1‑150 (MQ=255)
                        acacacCTCAGGTGCCAGCAGATGTCCGGAAATACCAATCACTTTTTCAGCATCAACGCCACCGTTCATCACTCGCGCCCGACTGTAGCCCACTTCGGCTCCCAGCTTTTCTGCCAGCATAGCTATTTCCTGGTTATCTGCCTCTCCGcc                                                                                                                          >  1:1149626/1‑150 (MQ=255)
                           cacCTCAGGTGCCAGCAGATGTCCGGAAATACCAATCACTTTTTCAGCATCAACGCCACCGTTCATCACTCGCGCCCGACTGTAGCCCACTTCGGCTCCCAGCTTTTCTGCCAGCATAGCTATTTCCTGGTTATCTGCCTCTCCGCCCTg                                                                                                                       <  1:4002536/150‑1 (MQ=255)
                           cacCTCAGGTGCCAGCAGATGTCCGGAAATACCAATCACTTTTTCAGCATCAACGCCACCGTTCATCACTCGCGCCCGACTGTAGCCCACTTCGGCTCCCAGCTTTTCTGCCAGCATAGCTATTTCCTGGTTATCTGCCTCTCCGCCCTg                                                                                                                       >  1:2697837/1‑150 (MQ=255)
                           cacCTCAGGTGCCAGCAGATGTCCGGAAATACCAATCACTTTTTCAGCATCAACGCCACCGTTCATCACTCGCGCCCGACTGTAGCCCACTTCGGCTCCCAGCTTTTCTGCCAGCATAGCTATTTCCTGGTTATCTGCCTCTCCGCCCTg                                                                                                                       <  2:3777440/150‑1 (MQ=255)
                                     gCCAGCAGATGTCCGGAAATACCAATCACTTTTTCAGCATCAACGCCACCGTTCATCACTCGCGCCCGACTGTAGCCCACTTCGGCTCCCAGCTTTTCTGCCAGCATAGCTATTTCCTGGTTATCTGCCTCTCCGCCCTGCCccaccacc                                                                                                             >  1:138078/1‑150 (MQ=255)
                                     gCCAGCAGATGTCCGGAAATACCAATCACTTTTTCAGCATCAACGCCACCGTTCATCACTCGCGCCCGACTGTAGCCCACTTCGGCTCCCAGCTTTTCTGCCAGCATAGCTATTTCCTGGTTATCTGCCTCTCCGCCCTGCCccaccacc                                                                                                             <  1:3776049/150‑1 (MQ=255)
                                           agATGTCCGGAAATACCAATCACTTTTTCAGCATCAACGCCACCGTTCATCACTCGCGCCCGACTGTAGCCCACTTCGGCTCCCAGCTTTTCTGCCAGCATAGCTATTTCCTGGTTATCTGCCTCTCCGCCCTGCCCCACCACCAGCAcc                                                                                                       <  1:4341044/150‑1 (MQ=255)
                                            gATGTCCGGAAATACCAATCACTTTTTCAGCATCAACGCCACCGTTCATCACTCGCGCCCGACTGTAGCCCACTTCGGCTCCCAGCTTTTCTGCCAGCATAGCTATTTCCTGGTTATCTGCCTCTCCGCCCTGCCCCACCACCAGCAccc                                                                                                      <  2:2451516/150‑1 (MQ=255)
                                            gATGTCCGGAAATACCAATCACTTTTTCAGCATCAACGCCACCGTTCATCACTCGCGCCCGACTGTAGCCCACTTCGGCTCCCAGCTTTTCTGCCAGCATAGCTATTTCCTGGTTATCTGCCTCTCCGCCCTGCCCCACCACCAGCAccc                                                                                                      >  2:519366/1‑150 (MQ=255)
                                             aTGTCCGGAAATACCAATCACTTTTTCAGCATCAACGCCACCGTTCATCACTCGCGCCCGACTGTAGCCCACTTCGGCTCCCAGCTTTTCTGCCAGCATAGCTATTTCCTGGTTATCTGCCTCTCCGCCCTGCCCCACCACCAGCACCCg                                                                                                     <  1:2894847/150‑1 (MQ=255)
                                             aTGTCCGGAAATACCAATCACTTTTTCAGCATCAACGCCACCGTTCATCACTCGCGCCCGACTGTAGCCCACTTCGGCTCCCAGATTTTCTGCCAGCATAGCTATTTCCTGGTTATCTGCCTCTCCGCCCTGCCCCACCACCAGCACCCg                                                                                                     >  2:3437495/1‑150 (MQ=255)
                                                tCCGGAAATACCAATCACTTTTTCAGCATCAACGCCACCGTTCATCACTCGCGCCCGACTGTAGCCCACTTCGGCTCCCAGCTTTTCTGCCAGCATAGCTATTTCCTGGTTATCTGCCTCTCCGCCCTGCCCCACCACCAGCACCCGTCt                                                                                                  >  2:4197902/1‑150 (MQ=255)
                                                tCCGGAAATACCAATCACTTTTTCAGCATCAACGCCACCGTTCATCACTCGCGCCCGACTGTAGCCCACTTCGGCTCCCAGCTTTTCTGCCAGCATAGCTATTTCCTGGTTATCTGCCTCTCCGCCCTGCCCCACCACCAGCACCCGTCt                                                                                                  <  1:825205/150‑1 (MQ=255)
                                                   ggAAATACCAATCACTTTTTCAGCATCAACGCCACCGTTCATCACTCGCGCCCGACTGTAGCCCACTTCGGCTCCCAGCTTTTCTGCCAGCATAGCTATTTCCTGGTTATCTGCCTCTCCGCCCTGCCCCACCACCAGCACCCGTCTGGc                                                                                               <  1:2169373/150‑1 (MQ=255)
                                                   ggAAATACCAATCACTTTTTCAGCATCAACGCCACCGTTCATCACTCGCGCCCGACTGTAGCCCACTTCGGCTCCCAGCTTTTCTGCCAGCATAGCTATTTCCTGGTTATCTGCCTCTCCGCCCTGCCCCACCACCAGCACCCGTCTGGc                                                                                               <  2:1560363/150‑1 (MQ=255)
                                                   ggAAATACCAATCACTTTTTCAGCATCAACGCCACCGTTCATCACTCGCGCCCGACTGTAGCCCACTTCGGCTCCCAGCTTTTCTGCCAGCATAGCTATTTCCTGGTTATCTGCCTCTCCGCCCTGCCCCACCACCAGCACCCGTCTGGc                                                                                               >  1:2031408/1‑150 (MQ=255)
                                                   ggAAATACCAATCACTTTTTCAGCATCAACGCCACCGTTCATCACTCGCGCCCGACTGTAGCCCACTTCGGCTCCCAGCTTTTCTGCCAGCATAGCTATTTCCTGGTTATCTGCCTCTCCGCCCTGCCCCACCACCAGCACCCGTCTGGc                                                                                               >  2:2964478/1‑150 (MQ=255)
                                                   ggAAATACCAATCACTTTTTCAGCATCAACGCCACCGTTCATCACTCGCGCCCGACTGTAGCCCACTTCGGCTCCCAGCTTTTCTGCCAGCATAGCTATTTCCTGGTTATCTGCCTCTCCGCCCTGCCCCACCACCAGCACCCGTCTGGc                                                                                               >  2:368490/1‑150 (MQ=255)
                                                   ggAAATACCAATCACTTTTTCAGCATCAACGCCACCGTTCATCACTCGCGCCCGACTGTAGCCCACTTCGGCTCCCAGCTTTTCTGCCAGCATAGCTATTTCCTGGTTATCTGCCTCTCCGCCCTGCCCCACCACCAGCACCCGTCTGGc                                                                                               <  2:553806/150‑1 (MQ=255)
                                                     aaaTACCAATCACTTTTTCAGCATCAACGCCACCGTTCATCACTCGCGCCCGACTGTAGCCCACTTCGGCTCCCAGCTTTTCTGCCAGCATAGCGATTTCCTGGTTATCTGCCTCTCCGCCCTGCCCCACCACCAGCACCCGTCTGGCCt                                                                                             <  1:608527/150‑1 (MQ=255)
                                                       aTACCAATCACTTTTTCAGCATCAACGCCACCGTTCATCACTCGCGCCCGACTGTAGCCCACTTCGGCTCCCAGCTTTTCTGCCAGCATAGCTATTTCCTGGTTATCTGCCTCTCCGCCCTGCCCCACCACCAGCACCCGTCTGGCCTCa                                                                                           <  1:1444021/150‑1 (MQ=255)
                                                       aTACCAATCACTTGTTCAGCATCAACGCCACCGTTCATCACTCGCGCCCGACTGTAGCCCACTTCGGCTCCCAGCTTTTCTGCCAGCATAGCTATTTCCTGGTTATCTGCCTCTCCGCCCTGCCCCACCACCAGCACCCGTCTGGCCTCa                                                                                           <  2:815835/150‑1 (MQ=255)
                                                        tACCAATCACTTTTTCAGCATCAACGCCACCGTTCATCACTCGCGCCCGACTGTAGCCCACTTCGGCTCCCAGCTTTTCTGCCAGCATAGCTATTTCCTGGTTATCTGCCTCTCCGCCCTGCCCCACCACCAGCACCCGTCTGGCCTCAg                                                                                          <  1:2355180/150‑1 (MQ=255)
                                                        tACCAATCACTTTTTCAGCATCAACGCCACCGTTCATCACTCGCGCCCGACTGTAGCCCACTTCGGCTCCCAGCTTTTCTGCCAGCATAGCTATTTCCTGGTTATCTGCCTCTCCGCCCTGCCCCACCACCAGCACCCGTCTGGCCTCAg                                                                                          <  1:535540/150‑1 (MQ=255)
                                                          ccAATCACTTTTTCAGCATCAACGCCACCGTTCATCACTCGCGCCCGACTGTAGCCCACTTCGGCTCCCAGCTTTTCTGCCAGCATAGCTATTTCCTGGTTATCTGCCTCTCCGCCCTGCCCCACCACCAGCACCCGTCTGGCCTCAGCt                                                                                        >  2:1502242/1‑150 (MQ=255)
                                                            aaTCACTTTTTCAGCATCAACGCCACCGTTCATCACTCGCGCCCGACTGTAGCCCACTTCGGCTCCCAGCTTTTCTGCCAGCATAGCTATTTCCTGGTTATCTGCCTCTCCGCCCTGCCCCACCACCAGCACCCGTCTGGCCTCAGCTaa                                                                                      >  1:3097643/1‑150 (MQ=255)
                                                            aaTCACTTTTTCAGCATCAACGCCACCGTTCATCACTCGCGCCCGACTGTAGCCCACTTCGGCTCCCAGCTTTTCTGCCAGCATAGCTATTTCCTGGTTATCTGCCTCTCCGCCCTGCCCCACCACCAGCACCCGTCTGGCCTCAGCTaa                                                                                      <  1:4254590/150‑1 (MQ=255)
                                                              tCACTTTTTCAGCATCAACGCCACCGTTCATCACTCGCGCCCGACTGTAGCCCACTTCGGCTCCCAGCTTTTCTGCCAGCATAGCTATTTCCTGGTTATCTGCCTCTCCGCCCTGCCCCACCACCAGCACCCGTCTGGCCTCAGCTAATg                                                                                    <  1:1373153/150‑1 (MQ=255)
                                                               cACTTTTTCAGCATCAACGCCACCGTTCATCACTCGCGCCCGACTGTAGCCCACTTCGGCTCCCAGCTTTTCTGCCAGCATAGCTATTTCCTGGTTATCTGCCTCTCCGCCCTGCCCCACCACCAGCACCCGTCTGGCCTCAGCTAATgg                                                                                   >  1:2860603/1‑150 (MQ=255)
                                                                  tttttCAGCATCAACGCCACCGTTCATCACTCGCGCCCGACTGTAGCCCACTTCGGCTCCCAGCTTTTCTGCCAGCATAGCTATTTCCTGGTTATCTGCCTCTCCGCCCTGCCCCACCACCAGCACCCGTCTGGCCTCAGCTAATGGGTc                                                                                <  1:4992448/150‑1 (MQ=255)
                                                                       cAGCATCAACGCCACCGTTCATCACTCGCGCCCGACTGTAGCCCACTTCGGCTCCCAGCTTTTCTGCCAGCATAGCTATTTCCTGGTTATCTGCCTCTCCGCCCTGCCCCACCACCAGCACCCGTCTGGCCTCAGCTAATGGGTCGCggg                                                                           >  1:1086029/1‑150 (MQ=255)
                                                                       cAGCATCAACGCCACCGTTCATCACTCGCGCCCGACTGTAGCCCACTTCGGCTCCCAGCTTTTCTGCCAGCATAGCTATTTCCTGGTTATCTGCCTCTCCGCCCTGCCCCACCACCAGCACCCGTCTGGCCTCAGCTAATGGGTCGCggg                                                                           <  2:2113835/150‑1 (MQ=255)
                                                                           aTCAACGCCACCGTTCATCACTCGCGCCCGACTGTAGCCCACTTCGGCTCCCAGCTTTTCTGCCAGCATAGCTATTTCCTGGTTATCTGCCTCTCCGCCCTGCCCCACCACCAGCACCCGTCTGGCCTCAGCTAATGGGTCGCGGGTAAc                                                                       <  1:1044922/150‑1 (MQ=255)
                                                                             cAACGCCACCGTTCATCACTCGCGCCCGACTGTAGCCCACTTCGGCTCCCAGCTTTTCTGCCAGCATAGCTATTTCCTGGTTATCTGCCTCTCCGCCCTGCCCCACCACCAGCACCCGTCTGGCCTCAGCTAATGGGTCGCGGGTAACAt                                                                     >  1:1219024/1‑150 (MQ=255)
                                                                              aaCGCCACCGTTCATCACTCGCGCCCGACTGTAGCCCACTTCGGCTCCCAGCTTTTCTGCCAGCATAGCTATTTCCTGGTTATCTGCCTCTCCGCCCTGCCCCACCACCAGCACCCGTCTGGCCTCAGCTAATGGGTCGCGGGTAACAtt                                                                    >  1:4101746/1‑150 (MQ=255)
                                                                                  ccACCGTTCATCACTCGCGCCCGACTGTAGCCCACTTCGGCTCCCAGCTTTTCTGCCAGCATAGCTATTTCCTGGTTATCTGCCTCTCCGCCCTGCCCCACCACCAGCACCCGTCTGGCCTCAGCTAATGGGTCGCGGGTAACATTTTTc                                                                >  2:1802678/1‑150 (MQ=255)
                                                                                   cACCGTTCATCACTCGCGCCCGACTGTAGCCCACTTCGGCTCCCAGCTTTTCTGCCAGCATAGCTATTTCCTGGTTATCTGCCTCTCCGCCCTGCCCCACCACCAGCACCCGTCTGGCCTCAGCTAATGGGTCGCGGGTAACATTTTTCa                                                               <  1:1964985/150‑1 (MQ=255)
                                                                                    aCCGTTCATCACTCGCGCCCGACTGTAGCCCACTTCGGCTCCCAGCTTTTCTGCCAGCATAGCGATTTCCTGGTTATCTGCCTCTCCGCCCTGCCCCACCACCAGCACCCGTCTGGCCTCAGCTAATGGGTCGCGGGTAACATTTTTCAg                                                              <  2:4805408/150‑1 (MQ=255)
                                                                                       gTTCATCACTCGCGCCCGACTGTAGCCCACTTCGGCTCCCAGCTTTTCTGCCAGCATAGCTATTTCCTGGTTATCTGCCTCTCCGCCCTGCCCCACCACCAGCACCCGTCTGGCCTCAGCTAATGGGTCGCGGGTAACATTTTTCAGATc                                                           >  1:3993185/1‑150 (MQ=255)
                                                                                          catcaCTCGCGCCCGACTGTAGCCCACTTCGGCTCCCAGCTTTTCTGCCAGCATAGCGATTTCCTGGTTATCTGCCTCTCCGCCCTGCCCCACCACCAGCACCCGTCTGGCCTCAGCTAATGGGTCGCGGGTAACATTTTTCAGATCctc                                                        >  1:3650365/1‑150 (MQ=255)
                                                                                           atcaCTCGCGCCCGACTGTAGCCCACTTCGGCTCCCAGCTTTTCTGCCAGCATAGCTATTTCCTGGTTATCTGCCTCTCCGCCCTGCCCCACCACCAGCACCCGTCTGGCCTCAGCTAATGGGTCGCGGGTAACATTTTTCAGATCctct                                                       >  2:19513/1‑150 (MQ=255)
                                                                                           atcaCTCGCGCCCGACTGTAGCCCACTTCGGCTCCCAGCTTTTCTGCCAGCATAGCTATTTCCTGGTTATCTGCCTCTCCGCCCTGCCCCACCACCAGCACCCGTCTGGCCTCAGCTAATGGGTCGCGGGTAACATTTTTCAGATCctct                                                       <  1:1602139/150‑1 (MQ=255)
                                                                                           atcaCTCGCGCCCGACTGTAGCCCACTTCGGCTCCCAGCTTTTCTGCCAGCATAGCTATTTCCTGGTTATCTGCCTCTCCGCCCTGCCCCACCACCAGCACCCGTCTGGCCTCAGCTAATGGGTCGCGGGTAACATTTTTCAGATCctct                                                       <  1:1009160/150‑1 (MQ=255)
                                                                                           atcaCTCGCGCCCGACTGTAGCCCACTTCGGCTCCCAGCTTTTCTGCCAGCATAGATATTTCCTGGTTATCTGCCTCTCCGCCCTGCCCCACCACCAGCACCCGTCTGGCCTCAGCTAATGGGTCGCGGGTAACATTTTTCAGATCctct                                                       <  2:3051634/150‑1 (MQ=255)
                                                                                             caCTCGCGCCCGACTGTAGCCCACTTCGGCTCCCAGCTTTTCTGCCAGCATAGCTATTTCCTGGTTATCTGCCTCTCCGCCCTGCCCCACCACCAGCACCCGTCTGGCCTCAGCTAATGGGTCGCGGGTAACATTTTTCAGATCCTCtgt                                                     >  2:4755757/1‑150 (MQ=255)
                                                                                              aCTCGCGCCCGACTGTAGCCCACTTCGGCTCCCAGCTTTTCTGCCAGCATAGCTATTTCCTGGTTATCTGCCTCTCCGCCCTGCCCCACCACCAGCACCCGTCTGGCCTCAGCTAATGGGTCGCGGGTAACATTTTTCAGATCCTCtgtg                                                    >  1:1696663/1‑150 (MQ=255)
                                                                                              aCTCGCGCCCGACTGTAGCCCACTTCGGCTCCCAGCTTTTCTGCCAGCATAGCGATTTCCTGGTTATCTGCCTCTCCGCCCTGCCCCACCACCAGCACCCGTCTGGCCTCAGCTAATGGGTCGCGGGTAACATTTTTCAGATCCTCtgtg                                                    <  1:4019159/150‑1 (MQ=255)
                                                                                                tCGCGCCCGACTGTAGCCCACTTCGGCTCCCAGCTTTTCTGCCAGCATAGCTATTTCCTGGTTATCTGCCTCTCCGCCCTGCCCCACCACCAGCACCCGTCTGGCCTCAGCTAATGGGTCGCGGGTAACATTTTTCAGATCCTCTGTGCt                                                  >  1:4716295/1‑150 (MQ=255)
                                                                                                tCGCGCCCGACTGTAGCCCACTTCGGCTCCCAGCTTTTCTGCCAGCATAGCTATTTCCTGGTTATCTGCCTCTCCGCCCTGCCCCACCACCAGCACCCGTCTGGCCTCAGCTAATGGGTCGCGGGTAACATTTTTCAGATCCTCTGTGCt                                                  <  2:4524970/150‑1 (MQ=255)
                                                                                                  gcgcCCGACTGTAGCCCACTTCGGCTCCCAGCTTTTCTGCCAGCATAGCTATTTCCTGGTTATCTGCCTCTCCGCCCTGCCCCACCACCAGCACCCGTCTGGCCTCAGCTAATGGGTCGCGGGTAACATTTTTCAGATCCTCTGTGCTaa                                                >  1:2086031/1‑150 (MQ=255)
                                                                                                   cgcCCGACTGTAGCCCACTTCGGCTCCCAGCTTTTCTGCCAGCATAGCTATTTCCTGGTTATCTGCCTCTCCGCCCTGCCCCACCACCAGCACCCGTCTGGCCTCAGCTAATGGGTCGCGGGTAACATTTTTCAGATCCTCTGTGCTAAc                                               <  1:3024456/150‑1 (MQ=255)
                                                                                                    gcCCGACTGTAGCCCACTTCGGCTCCCAGCTTTTCTGCCAGCATAGCTATTTCCTGGTTATCTGCCTCTCCGCCCTGCCCCACCACCAGCACCCGTCTGGCCTCAGCTAATGGGTCGCGGGTAACATTTTTCAGATCCTCTGTGCTAAcc                                              >  2:2658607/1‑150 (MQ=255)
                                                                                                      ccGACTGTAGCCCACTTCGGCTCCCAGCTTTTCTGCCAGCATAGCTATTTCCTGGTTATCTGCCTCTCCGCCCTGCCCCACCACCAGCACCCGTCTGGCCTCAGCTAATGGGTCGCGGGTAACATTTTTCAGATCCTCTGTGCTAAccag                                            >  2:633068/1‑150 (MQ=255)
                                                                                                        gACTGTAGCCCACTTCGGCTCCCAGCTTTTCTGCCAGCATAGCTATTTCCTGGTTATCTGCCTCTCCGCCCTGCCCCACCACCAGCACCCGTCTGGCCTCAGCTAATGGGTCGCGGGTAACATTTTTCAGATCCTCTGTGCTAAccagcc                                          >  2:3239250/1‑150 (MQ=255)
                                                                                                         aCTGTAGCCCACTTCGGCTCCCAGCTTTTCTGCCAGCATAGCTATTTCCTGGTTATCTGCCTCTCCGCCCTGCCCCACCACCAGCACCCGTCTGGCCTCAGCTAATGGGTCGCGGGTAACATTTTTCAGATCCTCTGTGCTAAccagcca                                         <  1:3206379/150‑1 (MQ=255)
                                                                                                          cTGTAGCCCACTTCGGCTCCCAGCTTTTCTGCCAGCATAGCTATTTCCTGGTTATCTGCCTCTCCGCCCTGCCCCACCACCAGCACCCGTCTGGCCTCAGCTAATGGGTCGCGGGTAACATTTTTCAGATCCTCTGTGCTAAccagccag                                        >  2:4441846/1‑150 (MQ=255)
                                                                                                          cTGTAGCCCACTTCGGCTCCCAGCTTTTCTGCCAGCATAGCTATTTCCTGGTTATCTGCCTCTCCGCCCTGCCCCACCACCAGCACCCGTCTGGCCTCAGCTAATGGGTCGCGGGTAACATTTTTCAGATCCTCTGTGCTAAccagccag                                        >  2:3865537/1‑150 (MQ=255)
                                                                                                           tGTAGCCCACTTCGGCTCCCAGCTTTTCTGCCAGCATAGCTATTTCCTGGTTATCTGCCTCTCCGCCCTGCCCCACCACCAGCACCCGTCTGGCCTCAGCTAATGGGTCGCGGGTAACATTTTTCAGATCCTCTGTGCTAACCAGCCAGt                                       >  2:1827300/1‑150 (MQ=255)
                                                                                                           tGTAGCCCACTTCGGCTCCCAGCTTTTCTGCCAGCATAGCTATTTCCTGGTTATCTGCCTCTCCGCCCTGCCCCACCACCAGCACCCGTCTGGCCTCAGCTAATGGGTCGCGGGTAACATTTTTCAGATCCTCTGTGCTAACCAGCCAGt                                       <  1:1451631/150‑1 (MQ=255)
                                                                                                           tGTAGCCCACTTCGGCTCCCAGCTTTTCTGCCAGCATAGCTATTTCCTGGTTATCTGCCTCTCCGCCCTGCCCCACCACCAGCACCCGTCTGGCCTCAGCTAATGGGTCGCGGGTAACATTTTTCAGATCCTCTGTGCTAACCAGCCAGt                                       >  1:2341463/1‑150 (MQ=255)
                                                                                                           tGTAGCCCACTTCGGCTCCCAGCTTTTCTGCCAGCATAGCTATTTCCTGGTTATCTGCCTCTCCGCCCTGCCCCACCACCAGCACCCGTCTGGCCTCAGCTAATGGGTCGCGGGTAACATTTTTCAGATCCTCTGTGCTAACCAGCCAGt                                       <  1:1658089/150‑1 (MQ=255)
                                                                                                            gTAGCCCACTTCGGCTCCCAGCTTTTCTGCCAGCATAGCTATTTCCTGGTTATCTGCCTCTCCGCCCTGCCCCACCACCAGCACCCGTCTGGCCTCAGCTAATGGGTCGCGGGTAACATTTTTCAGATCCTCTGTGCTAACCAGCCAGTc                                      <  2:2005098/150‑1 (MQ=255)
                                                                                                            gTAGCCCACTTCGGCTCCCAGCTTTTCTGCCAGCATAGCTATTTCCTGGTTATCTGCCTCTCCGCCCTGCCCCACCACCAGCACCCGTCTGGCCTCAGCTAATGGGTCGCGGGTAACATTTTTCAGATCCTCTGTGCTAACCAGCCAGTc                                      <  2:4865530/150‑1 (MQ=255)
                                                                                                                 ccACTTCGGCTCCCAGCTTTTCTGCCAGCATAGCTATTTCCTGGTTATCTGCCTCTCCGCCCTGCCCCACCACCAGCACCCGTCTGGCCTCAGCTAATGGGTCGCGGGTAACATTTTTCAGATCCTCTGTGCTAACCAGCCAGTCCGGaa                                 <  1:2459151/150‑1 (MQ=255)
                                                                                                                  cACTTCGGCTCCCAGCTTTTCTGCCAGCATAGCTATTTCCTGGTTATCTGCCTCTCCGCCCTGCCCCACCACCAGCACCCGTCTGGCCTCAGCTAATGGGTCGCGGGTAACATTTTTCAGATCCTCTGTGCTAACCAGCCAGTCCGGAAg                                <  2:4825448/150‑1 (MQ=255)
                                                                                                                        ggCTCCCAGCTTTTCTGCCAGCATAGCTATTTCCTGGTTATCTGCCTCTCCGCCCTGCCCCACCACCAGCACCCGTCTGGCCTCAGCTAATGGGTCGCGGGTAACATTTTTCAGATCCTCTGTGCTAACCAGCCAGTCCGGAAGCGCAcc                          >  1:1855611/1‑150 (MQ=255)
                                                                                                                        ggCTCCCAGCTTTTCTGCCAGCATAGCTATTTCCTGGTTATCTGCCTCTCCGCCCTGCCCCACCACCAGCACCCGTCTGGCCACAGCAAATGGGTCGCGGGTAACATTTTTCATATCCTCTGTGCTAACCAGCCAGTCCGGAAGCGCAcc                          >  1:3524325/1‑150 (MQ=255)
                                                                                                                         gCTCCCAGCTTTTCTGCCAGCATAGCTATTTCCTGGTTATCTGCCTCTCCGCCCTGCCCCACCACCAGCACCCGTCTGGCCTCAGCTAATGGGTCGCGGGTAACATTTTTCAGATCCTCTGTGCTAACCAGCCAGTCCGGAAGCGCAccc                         <  1:3599039/150‑1 (MQ=255)
                                                                                                                         gCTCCCAGCTTTTCTGCCAGCATAGCTATTTCCTGGTTATCTGCCTCTCCGCCCTGCCCCACCACCAGCACCCGTCTGGCCTCAGCTAATGGGTCGCGGGTAACATTTTTCAGATCCTCTGTGCTAACCAGCCAGTCCGGAAGCGCAccc                         <  1:899167/150‑1 (MQ=255)
                                                                                                                          cTCCCAGCTTTTCTGCCAGCATAGCTATTTCCTGGTTATCTGCCTCTCCGCCCTGCCCCACCACCAGCACCCGTCTGGCCTCAGCTAATGGGTCGCGGGTAACATTTTTCAGATCCTCTGTGCTAACCAGCCAGTCCGGAAGCGCACCCg                        >  2:3357099/1‑150 (MQ=255)
                                                                                                                           tCCCAGCTTTTCTGCCAGCATAGCTATTTCCTGGTTATCTGCCTCTCCGCCCTGCCCCACCACCAGCACCCGTCTGGCCTCAGCTAATGGGTCGCGGGTAACATTTTTCAGATCCTCTGTGCTAACCAGCCAGTCCGGAAGCGCACCCgg                       <  1:3299527/150‑1 (MQ=255)
                                                                                                                           tCCCAGCTTTTCTGCCAGCATAGCTATTTCCTGGTTATCTGCCTCTCCGCCCTGCCCCACCACCAGCACCCGTCTGGCCTCAGCTAATGGGTCGCGGGTAACATTTTTCAGATCCTCTGTGCTAACCAGCCAGTCCGGAAGCGCACCCgg                       <  1:177196/150‑1 (MQ=255)
                                                                                                                            cccAGCTTTTCTGCCAGCATAGCTATTTCCTGGTTATCTGCCTCTCCGCCCTGCCCCACCACCAGCACCCGTCTGGCCTCAGCTAATGGGTCGCGGGTAACATTTTTCAGATCCTCTGTGCTAACCAGCCAGTCCGGAAGCGCACCCggg                      >  1:3324793/1‑150 (MQ=255)
                                                                                                                            cccAGCTTTTCTGCCAGCATAGCTATTTCCTGGTTATCTGCCTCTCCGCCCTGCCCCACCACCAGCACCCGTCTGGCCTCAGCTAATGGGTCGCGGGTAACATTTTTCAGATCCTCTGTGCTAACCAGCCAGTCCGGAAGCGCACCCggg                      <  2:274737/150‑1 (MQ=255)
                                                                                                                              cAGCTGTTCTGCCAGCATAGCTATTTCCTGGTTATCTGCCTCTCCGCCCTGCCCCACCACCAGCACCCGTCTGGCCTCAGCTAATGGGTCGCGGGTAACATTTTTCAGATCCTCTGTGCTAACCAGCCAGTCCGGAAGCGCACCCGGGAc                    <  2:2245875/150‑1 (MQ=255)
                                                                                                                                   tttCTGCCAGCATAGCTATTTCCTGGTTATCTGCCTCTCCGCCCTGCCCCACCACCAGCACCCGTCTGGCCTCAGCTAATGGGTCGCGGGTAACATTTTTCAGATCCTCTGTGCTAACCAGCCAGTCCGGAAGCGCACCCGGGACAATGt               >  2:732476/1‑150 (MQ=255)
                                                                                                                                      cTGCCAGCATAGCTATTTCCTGGTTATCTGCCTCTCCGCCCTGCCCCACCACCAGCACCCGTCTGGCCTCAGCTAATGGGTCGCGGGTAACATTTTTCAGATCCTCTGTGCTAACCAGCCAGTCCGGAAGCGCACCCGGGACAATGTTCa            <  1:48158/150‑1 (MQ=255)
                                                                                                                                      cTGCCAGCATAGCTATTTCCTGGTTATCTGCCTCTCCGCCCTGCCCCACCACCAGCACCCGTCTGGCCTCAGCTAATGGGTCGCGGGTAACATTTTTCAGATCCTCTGTGCTAACCAGCCAGTCCGGAAGCGCACCCGGGACAATGTTCa            >  1:486109/1‑150 (MQ=255)
                                                                                                                                       tGCCAGCATAGCTATTTCCTGGTTATCTGCCTCTCCGCCCTGCCCCACCACCAGCACCCGTCTGGCCTCAGCTAATGGGTCGCGGGTAACATTTTTCAGATCCTCTGTGCTAACCAGCCAGTCCGGAAGCGCACCCGGGACACTGTTCAg           >  2:3604690/1‑150 (MQ=255)
                                                                                                                                        gCCAGCATAGCTATTTCCTGGTTATCTGCCTCTCCGCCCTGCCCCACCACCAGCACCCGTCTGGCCTCAGCTAATGGGTCGCGGGTAACATTTTTCAGATCCTCTGTGCTAACCAGCCAGTCCGGAAGCGCACCCGGGACAATGTTCAgt          >  2:1298239/1‑150 (MQ=255)
                                                                                                                                         ccAGCATAGCTATTTCCTGGTTATCTGCCTCTCCGCCCTGCCCCACCACCAGCACCCGTCTGGCCTCAGCTAATGGGTCGCGGGTAACATTTTTCAGATCCTCTGTGCTAACCAGCCAGTCCGGAAGCGCACCCGGGACAATGTTCAgtt         <  1:2748597/150‑1 (MQ=255)
                                                                                                                                          cAGCATAGCTATTTACTGGTTATCTGCCTCTCCGCCCTGCCCCACAACCAGCACCCGTCTGGCCTCAGCTAATGGGTCGCGGGTAACATTTTTCAGATCCTCTGTGCTAACCAGCCAGTCCGGAAGCTCACCCGGGACAATGATCAgttg        >  1:1592991/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
TGCGCCAGAAGCGCCAACAACAATACACACCTCAGGTGCCAGCAGATGTCCGGAAATACCAATCACTTTTTCAGCATCAACGCCACCGTTCATCACTCGCGCCCGACTGTAGCCCACTTCGGCTCCCAGCTTTTCTGCCAGCATAGCGATTTCCTGGTTATCTGCCTCTCCGCCCTGCCCCACCACCAGCACCCGTCTGGCCTCAGCTAATGGGTCGCGGGTAACATTTTTCAGATCCTCTGTGCTAACCAGCCAGTCCGGAAGCGCACCCGGGACAATGTTCAGTTGTTGCAT  >  CP009273/2888437‑2888730

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑