Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,901,394 C→A 6.8% A544A (GCG→GCT)  pyrG ← CTP synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,901,3940CA 6.8% 1016.3 / 33.5 265A544A (GCG→GCT) pyrGCTP synthetase
Reads supporting (aligned to +/- strand):  ref base C (134/113);  new base A (12/6);  total (146/119)
Fisher's exact test for biased strand distribution p-value = 3.38e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.91e-01

Only 100 of 273 total aligned reads displayed.

GGGTTACCACGGGAGTCGATGATTTCACGACCGATGATTTTTACGATTTTGGACATTAGGTTTTCCTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTGGAGTAGAAGTAAACTCCGGATGGAACTGGCAAGCCACGAACCACGGGTGATTCGGAACTTCGATGATCTCGACCAACTGATC  >  CP009273/2901245‑2901543
                                                                                                                                                     |                                                                                                                                                     
gggTTACCACGGGAGTCGATGATTTCACGACCGATGATTTTTACGATTTTGGACATTAGGTTTTCCTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTc                                                                                                                                                       <  1:1598452/150‑1 (MQ=255)
    tACCACGGGAGTCGATGATTTCACGACCGATGATTTTTACGATTTTGGACATTAGGTTTTCCTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTACGCGTTGCCGCTCTAACTGTTTTACTTACTTCGCCt                                                                                                                                                   >  2:2786804/1‑150 (MQ=255)
    tACCACGGGAGTCGATGATTTCACGACCGATGATTTTTACGATTTTGGACATTAGGTTTTCCTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTACGCGTTGCCGCGCTAACTTTTTTACTTACTTCGCCt                                                                                                                                                   >  2:3889165/1‑150 (MQ=255)
     aCCACGGGAGTCTATTATTTCACGACCGATTATTTTTACGATTTTGGACATTTGTTTTTCCTCAAGTCACTAGTTAAACTTAAACTCCAGACAAACAACGCGTACCCAGGGTACGCTTTGCCGCTCTAACTTTTTTACTTACTTCGCCTg                                                                                                                                                  <  2:171850/150‑1 (MQ=255)
      ccACGGGAGTCGATGATTTCACGACCGATGATTTTTACGATTTTGGACATTAGGTTTTCCTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGa                                                                                                                                                 <  1:1918581/150‑1 (MQ=255)
       cACGGGAGTCGATGATTTCACGACCGATGATTTTTACGATTTTGGACATTCGGTTTTCCTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTCCCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGAc                                                                                                                                                <  1:4746009/150‑1 (MQ=255)
       cACGGGAGTCGATGATTTCACGACCGATGATTTTTACGATTTTGGACATTAGGTTTTCCTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGAc                                                                                                                                                <  2:179287/150‑1 (MQ=255)
        aCGGGAGTCGATGATTTCACGACCGATGATTTTTACGATTTTGGACCTTAGGTTTTCCTCAAGTCCCTAGTTAAACTGAAACTCCCGACAAACAACGCGTACCCAGGGTACGCGTTGCCGCGCTAACTTTTTTACTTACGTCGCCTGACg                                                                                                                                               >  1:303193/1‑150 (MQ=255)
          gggAGTCGATGATTTCACGACCGATGATTTTTACGATTTTGGACATGAGGTTTTCCTCAAGTCACTAGTTACACTGAAACTCCAGACAAACAACGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGtt                                                                                                                                             <  2:1990480/150‑1 (MQ=255)
           ggAGTCGATGATTTCACGACCGATGATTTTTACGATTTTGGACATTAGGTTTTCCTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTACGCGTTGCCGCTCTAAATTTTTTACTTACTTCGCCGGACGttt                                                                                                                                            >  1:3787197/1‑150 (MQ=255)
              gTCGATGATTTCACGACCGATGATTTTTACGATTTTGGACATTAGGTTTTCCTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGTGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTg                                                                                                                                         >  2:2529374/1‑150 (MQ=255)
               tCGATGATTTCACGACCGATGATTTTTACGATTTTGGACATTAGGTTTTCCTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTgg                                                                                                                                        <  2:2422729/150‑1 (MQ=255)
                cGATGATTTCACGACCGATGATTTTTACGATTTTGGACATTAGGTTTTCCTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGa                                                                                                                                       >  2:4658090/1‑150 (MQ=255)
                cGATGATTTCACGACCGATGATTTTTACGATTTTGGACATTAGGTTTTCCTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGa                                                                                                                                       <  2:2579034/150‑1 (MQ=255)
                  atgatTTCACGACCGATGATTTTTACGATTTTGGACATTAGGTTTTCCTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAAc                                                                                                                                     >  1:1682175/1‑150 (MQ=255)
                   tgatTTCACGACCGATGATTTTTACGATTTTGGACATTAGGTTTTCCTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACt                                                                                                                                    <  1:3590070/150‑1 (MQ=255)
                       ttCACGACCGATGATTTTTACGATTTTGGACATTAGGTTTTCCTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCt                                                                                                                                >  2:797338/1‑150 (MQ=255)
                          aCGACCGATGATTTTTACGATTTTGGACATTAGGTTTTCCTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTggc                                                                                                                             <  1:3671583/150‑1 (MQ=255)
                            gACCGATGATTTTTACGATTTTGGACATTAGGTTTTCCTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTggcgg                                                                                                                           >  1:473921/1‑150 (MQ=255)
                             aCCGATGATTTTTACGATTTTGGACATTAGGTTTTCCTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACCACGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTggcggc                                                                                                                          <  1:588464/150‑1 (MQ=255)
                                gatgatTTTTACGATTTTGGACATTAGGTTTTCCTCAAGTCACTAGTTAAACTGAAACTCCAGACAATCAACGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCttt                                                                                                                       >  1:4433649/1‑150 (MQ=255)
                                    atTTTTACTATTTTGGACATTAGGTTTTCCTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTcaca                                                                                                                   <  2:27421/150‑1 (MQ=255)
                                    atTTTTACGATTTTGGACATTAGGTTTTCCTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTcaca                                                                                                                   >  2:1127869/1‑150 (MQ=255)
                                      ttttACGATTTTGGACATTAGGTTTTCCTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACaaa                                                                                                                 >  2:1593350/1‑150 (MQ=255)
                                      ttttACGATTTTGGACATTAGGTTTTCCTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACaaa                                                                                                                 <  2:3270234/150‑1 (MQ=255)
                                          aCGATTTTGGACATTAGGTTTTCCTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCt                                                                                                             >  1:2363937/1‑150 (MQ=255)
                                          aCGATTTTGGACAGGAGGTTTTCCTCAAGTCACTAGTTAAACTGACACTCCAGACAAACAACGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCt                                                                                                             <  2:3112720/150‑1 (MQ=255)
                                           cGATTTTGGACATTAGGTTTTCCTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTg                                                                                                            <  2:317087/150‑1 (MQ=255)
                                             aTTTTGGACATTAGGTTTTCCTCAAGTCACTAGTTAAACTGAAACTCAAGACAAACAAAGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCa                                                                                                          >  1:1549832/1‑150 (MQ=255)
                                                 tGGACATTAGGTTTTCCTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACa                                                                                                      >  2:5029872/1‑150 (MQ=255)
                                                   gACATTAGGTTTGCCTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGc                                                                                                    <  1:3868905/150‑1 (MQ=255)
                                                    aCATTAGGTTTTCCTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCg                                                                                                   <  2:3046580/150‑1 (MQ=255)
                                                    aCATTAGGTTTTCCTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTAGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCg                                                                                                   >  1:3580746/1‑150 (MQ=255)
                                                        tAGGTTTTCCTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTAGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACCAAGCCTGCAAACAGCGGGTg                                                                                               >  2:4700086/1‑150 (MQ=255)
                                                          ggTTTTCCTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTACGCGTTGCCGCTCTACCTTTTTTACTTACTTCGCCTGACGTTTCTGGCACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGAc                                                                                             >  1:2924012/1‑150 (MQ=255)
                                                           gTTTTCCTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACACCGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGAcc                                                                                            <  1:185837/150‑1 (MQ=255)
                                                               tCCTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCa                                                                                        >  2:2703637/1‑150 (MQ=255)
                                                               tCCTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTACGCGTTGCCGCTCTAACATTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCa                                                                                        <  2:3964317/150‑1 (MQ=255)
                                                                 cTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACg                                                                                      >  2:1437987/1‑150 (MQ=255)
                                                                 cTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACg                                                                                      >  2:2173947/1‑150 (MQ=255)
                                                                   cAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTg                                                                                    >  1:2216623/1‑150 (MQ=255)
                                                                   cAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTg                                                                                    <  1:4197112/150‑1 (MQ=255)
                                                                          cTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTGGAGTAGa                                                                             >  2:62869/1‑150 (MQ=255)
                                                                            aGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTGGAGTAGAAg                                                                           >  2:3785269/1‑150 (MQ=255)
                                                                               tAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTGGAGTAGAAGTaa                                                                        >  1:2656741/1‑150 (MQ=255)
                                                                               tAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACGTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTGGAGTAGAAGTaa                                                                        <  2:406682/150‑1 (MQ=255)
                                                                                aaaCTGAAACTCCAGACAAACAACGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTGGAGTAGAAGTaaa                                                                       <  2:2480827/150‑1 (MQ=255)
                                                                                aaaCTGAAACTCCAGACAAACAACGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTGGAGTAGAAGTaaa                                                                       >  1:1404918/1‑150 (MQ=255)
                                                                                 aaCTGAAACTCCAGACAAACAACGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTGGAGTAGAAGTAAAc                                                                      <  2:4501054/150‑1 (MQ=255)
                                                                                  aCTGAAACTCCAGACAAACAACGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTGGAGTAGAAGTAAACt                                                                     >  1:4016878/1‑150 (MQ=255)
                                                                                    ttaaaCTCCAGACAAACAACGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCTCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTGGAGTAGAAGTAAACTcc                                                                   <  1:583508/148‑1 (MQ=255)
                                                                                     gAAACTCCAGACAAACAACGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTGGAGTAGAAGTAAACTCCg                                                                  <  1:3358864/150‑1 (MQ=255)
                                                                                      aaaCTCCAGACAAACAACGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTGGAGTAGAAGTAAACTCCgg                                                                 >  2:5097253/1‑150 (MQ=255)
                                                                                       aaCTCCAGACAAACAACGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTGGAGTAGAAGTAAACTCCGGa                                                                <  1:2036710/150‑1 (MQ=255)
                                                                                        aCTCCAGACAAACCCCGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTGGAGTAGAAGTAAACTCCGGAt                                                               <  1:5020056/150‑1 (MQ=255)
                                                                                             aGACAAACAACGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTGGAGTAGAAGTCAACTCCGGATGGAAc                                                          >  2:4505407/1‑150 (MQ=255)
                                                                                              gACAAACAACGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTGGAGTAGAAGTAAACTCCGGATGGAACt                                                         <  1:1989792/150‑1 (MQ=255)
                                                                                               acaaacaaCGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTGGAGTAGAAGTAAACTCCGGATGGAACTg                                                        >  2:4451708/1‑150 (MQ=255)
                                                                                                 aaacaaCGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTGGAGTAGAAGTAAACTCCGGATGGAACTGGc                                                      >  2:715047/1‑150 (MQ=255)
                                                                                                 aaacaaCGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTGGAGTAGAAGTAAACTCCGGATGGAACTGGc                                                      >  1:2159867/1‑150 (MQ=255)
                                                                                                    caacGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCCTCACGTGGAGTAGCAGTAAACTCCGGATGGAACTGGCAAg                                                   >  1:3943330/1‑150 (MQ=255)
                                                                                                     aacGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTGGAGTAGAAGTAAACTCCGGATGGAACTGGCAAGc                                                  >  1:3201550/1‑150 (MQ=255)
                                                                                                     aacGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTGGAGTAGAAGTAAACTCCGGATGGAACTGGCAAGc                                                  >  1:3370320/1‑150 (MQ=255)
                                                                                                     aacGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTGGAGTAGAAGTAAACTCCGGATGGAACTGGCAAGc                                                  >  2:744553/1‑150 (MQ=255)
                                                                                                     aacGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTAGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTGGAGTAGAAGTAAACTCCGGATGGAACTGGCAAGc                                                  >  1:3291967/1‑150 (MQ=255)
                                                                                                        gcgTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTAGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTGGAGTAGAAGTAAACTCCGGATGGAACTGGCAAGCCAc                                               <  2:4175472/150‑1 (MQ=255)
                                                                                                         cgTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTGGAGTAGAAGTAAACTCCGGATGGAACTGGCAAGCCACg                                              >  2:4127511/1‑150 (MQ=255)
                                                                                                          gTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTGGAGTAGAAGTAAACTCCGGATGGAACTGGCAAGCCACGa                                             >  2:1322698/1‑150 (MQ=255)
                                                                                                          gTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTGGAGTAGAAGTAAACTCCGGATGGAACTGGCAAGCCACGa                                             <  2:4379350/150‑1 (MQ=255)
                                                                                                          gTACCCAGGGTACGCGTTGCCGCTCTAACTTTTGTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGGGACCATCACGGGGAGAAGAAGTAAACTCCGGATGGACCTGGCACGCCCCGa                                             >  2:2042638/1‑150 (MQ=255)
                                                                                                          gTACCCAGGGGACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTGGAGTAGAAGTAAACTCCGGATGGAACTGGCAAGCCACGa                                             <  2:2932632/150‑1 (MQ=255)
                                                                                                           tACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTGGAGTAGAAGTAAACTCCGGATGGAACTGGCAAGCCACGaa                                            >  2:4705334/1‑150 (MQ=255)
                                                                                                           tACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTGGAGTAGAAGTAAACTCCGGATGGAACTGGCAAGCCACGaa                                            <  1:4126223/150‑1 (MQ=255)
                                                                                                            aCCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTGGAGTAGAAGTAAACTCCGGATGGAACTGGCAAGCCACGAAc                                           <  2:1945900/150‑1 (MQ=255)
                                                                                                             cccAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTGGAGTAGAAGTAAACTCCGGATGGAACTGGCAAGCCACGAAcc                                          >  2:2635919/1‑150 (MQ=255)
                                                                                                              ccAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTGGAGTAGAAGTAAACTCCGGATGGAACTGGCAAGCCACGAACCa                                         <  1:125686/150‑1 (MQ=255)
                                                                                                               cAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTGGAGTAGAAGTAAACTCCGGATGGAACTGGCAAGCCACGAACCAc                                        >  2:3461990/1‑150 (MQ=255)
                                                                                                                 gggTACGCGGTGCCGCTCTAACTTTTTGACGTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTGGAGTAGAAGTAAACTCCGGATGGAACTGGCAAGCCACGAACCACgg                                      <  2:1086794/150‑1 (MQ=255)
                                                                                                                  ggTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTGGAGTAGAAGTAAACTCCGGATGGAACTGGCAAGCCACGAACCACggg                                     >  1:2429360/1‑150 (MQ=255)
                                                                                                                      cgcgTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTGGAGTAGAAGTAAACTCCGGATGGAACTGGCAAGCCACGAACCACGGGTGag                                 >  2:4608450/1‑149 (MQ=255)
                                                                                                                      cgcgTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTGGAGTAGAAGTAAACTCCGGATGGAACTGGCAAGCCACGAACCACGGGTGAt                                 >  2:2940429/1‑150 (MQ=255)
                                                                                                                      cgcgTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTGGAGTAGAAGTAAACTCCGGATGGAACTGGCAAGCCACGAACCACGGGTGAt                                 >  2:1316512/1‑150 (MQ=255)
                                                                                                                       gcgTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTGGAGTAGAAGTAAACTCCGGATGGAACTGGCAAGCCACGAACCACGGGTGAtt                                <  1:410194/150‑1 (MQ=255)
                                                                                                                        cgTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTGGAGTAGAAGTAAACTCCGGATGGAACTGGCAAGCCACGAACCACGGGTGATTc                               <  2:473921/150‑1 (MQ=255)
                                                                                                                            gCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTGGAGTAGAAGTAAACTCCGGATGGAACTGGCAAGCCACGAACCACGGGTGATTCGGaa                           >  1:1296567/1‑150 (MQ=255)
                                                                                                                             ccGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTGGAGTAGAAGTAAACTCCGGATGGAACTGGCAAGCCACGAACCACGGGTGATTCGGAAc                          <  2:4999322/150‑1 (MQ=255)
                                                                                                                                ctctAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTGGAGTAGAAGTAAACTACGGATGGAACTGGCAAGCCACGAACCACGGGTGATTCGGAACTTc                       >  2:3630103/1‑150 (MQ=255)
                                                                                                                                    aaCTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTGGAGTAGAAGTAAACTCCGGATGGAACTGGCAAGCCACGAACCACGGGTGATTCGGAACTTCgatg                   >  1:3714118/1‑150 (MQ=255)
                                                                                                                                    aaCTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTGGAGTAGAAGTAAACTCCGGATGGAACTGGCAAGCCACGAACCACGGGTGATTCGGAACTTCgatg                   >  1:1545006/1‑150 (MQ=255)
                                                                                                                                      cTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTGGAGTAGAAGTAAACTCCGGATGGAACTGGCAAGCCACGAACCACGGGTGATTCGGAACTTCgatgat                 <  1:431029/150‑1 (MQ=255)
                                                                                                                                      cTTTTTTACTTACTTAGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTGGAGTAGAAGTAAACTCCGGATGGAACTGGCAAGCCACGAACCACGGGTGATTCGGAACTTCgatgat                 >  1:2114471/1‑150 (MQ=255)
                                                                                                                                       ttttttACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTGGAGTAGAAGTAAACTCCGGATGGAACTGGCAAGCCACGAACCACGGGTGATTCGGAACTTCGATGAtc                >  1:4489425/1‑150 (MQ=255)
                                                                                                                                             acttacttCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTGGAGTAGAAGTAAACTCCGGATGGAACTGGCAAGCCACGAACCACGGGTGATTCGGAACTTCGATGATCTCGAcc          <  1:440378/150‑1 (MQ=255)
                                                                                                                                               ttacttCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTGGAGTAGAAGTAAACTCCGGATGGAACTGGCAAGCCACGAACCACGGGTGATTCGGAACTTCGATGATCTCGACCaa        >  2:4283516/1‑150 (MQ=255)
                                                                                                                                                  cttCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTGGAGTAGAAGTAAACTCCGGATGGAACTGGCAAGCCACGAACCACGGGTGATTCGGAACTTCGATGATCTCGACCAACTg     >  1:5113395/1‑150 (MQ=255)
                                                                                                                                                   ttCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTGGAGTAGAAGTAAACTCCGGATGGAACTGGCAAGCCACGAACCACGGGTGATTCGGAACTTCGATGATCTCGACCAACTGa    >  1:857364/1‑150 (MQ=255)
                                                                                                                                                   ttCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTGGAGTAGAAGTAAACTCCGGATGGAACTGGCAAGCCACGAACCACGGGTGATTCGGAACTTCGATGATCTCGACCAACTGa    >  2:484130/1‑150 (MQ=255)
                                                                                                                                                   ttCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTGGAGTAGAAGTAAACTCCGGATGGAACTGGCAAGCCACGAACCACGGGTGATTCGGAACTTCGATGATCTCGACCAACTGa    >  2:484216/1‑150 (MQ=255)
                                                                                                                                                   ttCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTGGAGTAGAAGTAAACTCCGGATGGAACTGGCAAGCCACGAACCACGGGTGATTCGGAACTTCGATGATCTCGACCAACTGa    >  2:934365/1‑150 (MQ=255)
                                                                                                                                                     cGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTGGAGTAGAAGTAAACTCCGGATGGAACTGGCAAGCCACGAACCACGGGTGATTCGGAACTTCGATGATCTCGACCAACTGatc  <  1:300831/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
GGGTTACCACGGGAGTCGATGATTTCACGACCGATGATTTTTACGATTTTGGACATTAGGTTTTCCTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTGGAGTAGAAGTAAACTCCGGATGGAACTGGCAAGCCACGAACCACGGGTGATTCGGAACTTCGATGATCTCGACCAACTGATC  >  CP009273/2901245‑2901543

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑