Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,959,004 G→A 100% I131I (ATC→ATT)  lgt ← phosphatidylglycerol‑prolipoprotein diacylglyceryl transferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,959,0040GA95.6% 894.9 / 22.0 252I131I (ATC→ATT) lgtphosphatidylglycerol‑prolipoprotein diacylglyceryl transferase
Reads supporting (aligned to +/- strand):  ref base G (4/7);  new base A (123/118);  total (127/125)
Fisher's exact test for biased strand distribution p-value = 3.74e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.11e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 252 total aligned reads displayed.

TTGGTTTGCAGCAGCAAAATATCTTCTGTACGGGAGCCAGGGAACAGCATGGCAAACGGGAAGTTCGGGTCAACGCGGCCCCACAATTCACCGTTAATAAAGTTGCCCAGACGCCCGGCACCAAGACCAAACGGAATGAGTGGTGCGATAAAATCAGAGACCTGGAAGAAGGAACGTTTAGTACGGCGGGCGAAGATAATCATCACCACGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGTCCCAGACACGGAACAGATACAGCGGATCGGCCATAAACTGCGG  >  CP009273/2958858‑2959150
                                                                                                                                                  |                                                                                                                                                  
 tggttTGCAGCAGCAAAATATCTTCTGTACGGGAGCCAGGGAACAGCATGGCAAACGGGAAGTTCGGGTCAACGCGGCCCCACAATTCACCGTTAATAAAGTTGCCCAGACGCCCGGCACCAAGACCAAACGGAATGAGTGGTGCAATaa                                                                                                                                                >  2:2687350/1‑150 (MQ=255)
  ggttTGCAGCAGCAAAATATCTTCTGTACGGGAGCCAGGGAACAGCATGGCAAACGGGAAGTTCGGGTCAACGCGGCCCCACAATTCACCGTTAATAAAGTTGCCCAGACGCCCGGCACCAAGACCAAACGGAATGAGTGGTGCAATaaa                                                                                                                                               >  1:2419498/1‑150 (MQ=255)
   gttTGCAGCAGCAAAATATCTTCTGTACGGGAGCCAGGGAACAGCATGGCAAACGGGAAGTTCGGGTCAACGCGGCCCCACAATTCACCGTTAATAAAGTTGCCCAGACGCCCGGCACCAAGACCAAACGGAATGAGTGGTGCAATaaaa                                                                                                                                              <  2:4189424/150‑1 (MQ=255)
   gttTGCAGCAGCAAAAGATCTTCTGTACGGGAGCCAGGGAACAGCATGGCAAACGGGAAGTTCGGGTCAACGCGGCCCCACAATTCACCGTTAATAAAGTTGCCCAGACGCCCGGCACCAAGACCAAACGGAATGAGTGGTGCAATaaaa                                                                                                                                              <  2:3410988/150‑1 (MQ=255)
    tttGCAGCAGCAAAATATCTTCTGTACGGGAGCCAGGGAACAGCATGGCAAACGGGAAGTTCGGGTCAACGCGGCCCCACAATTCACCGTTAATAAAGTTGCCCAGACGCCCGGCACCAAGACCAAACGGAATGAGTGGTGCAATAAAAt                                                                                                                                             <  2:1051704/150‑1 (MQ=255)
      tGCAGCAGCAAAATATCTTCTGTACGGGAGCCAGGGAACAGCATGGCAAACGGGAAGTTCGGGTCAACGCGGCCCCACAATTCACCGTTAATAAAGTTGCCCAGACGCCCGGCACCAAGACCAAACGGACTGAGTGGTGCAATAAAATCa                                                                                                                                           >  2:1248177/1‑150 (MQ=255)
         agcagcaAAATATCTTCTGTACGGGAGCCAGGGAACAGCATGGCAAACGGGAAGTTCGGGTCAACGCGGCCCCACAATTCACCGTTAATAAAGTTGCCCAGACGCCCGGCACCAAGACCAAACGGAATGAGTGGTGCAATAAAATCagag                                                                                                                                        <  1:1484539/150‑1 (MQ=255)
         agcagcaAAATATCTTCTGTACGGGAGCCAGGGAACAGCATGGCAAACGGGAAGTTCGGGTCAACGCGGCCCCACAATTCACCGTTAATAAAGTTGCCCAGACGCCCGGCACCAAGACCAAACGGAATGAGTGGTGCAATAAAATCagag                                                                                                                                        <  1:4122483/150‑1 (MQ=255)
           cagcaAAATATCTTCTGTACGGGAGCCAGGGAACAGCATGGCAAACGGGAAGTTCGGGTCAACGCGGCCCCACAATTCACCGTTAATAAAGTTGCCCAGACGCCCGGCACCAAGACCAAACGGAATGAGTGGTGCAATAAAATCAGAGAc                                                                                                                                      <  2:4111263/150‑1 (MQ=255)
              caAAATATCTTCTGTACGGGAGCCAGGGAACAGCATGGCAAACGGGAAGTTCGGGTCAACGCGGCCCCACAATTCACCGTTAATAAAGTTGCCCAGACGCCCGGCACCAAGACCAAACGGAATGAGTGGTGCAATAAAATCAGAGACCTg                                                                                                                                   >  2:3639259/1‑150 (MQ=255)
                 aaTATCTTCTGTACGGGAGCCAGGGAACAGCATGGCAAACGGGAAGTTCGGGTCAACGCGGCCCCACAATTCACCGTTAATAAAGTTGCCCAGACGCCCGGCACCAAGACCAAACGGAATGAGTGGTGCAATAAAATCAGAGACCTGgaa                                                                                                                                <  1:1047751/150‑1 (MQ=255)
                 aaTATCTTCTGTACGGGAGCCAGGGAACAGCATGGCAAACGGGAAGTTCGGGTCAACGCGGCCCCACAATTCACCGTTAATAAAGTTGCCCAGACGCCCGGCACCAAGACCAAACGGAATGAGTGGTGCAATAAAATCAGAGACCTGgaa                                                                                                                                >  2:3080952/1‑150 (MQ=255)
                   tatCTTCTGTACGGGAGCCAGGGAACAGCATGGCAAACGGGAAGTTCGGGTCAACGCGGCCCCACAATTCACCGTTAATAAAGTTGCCCAGACGCCCGGCACCAAGACCAAACGGAATGAGTGGTGCAATAAAATCAGAGACCTGgaaga                                                                                                                              <  1:1214773/150‑1 (MQ=255)
                   tatCTTCTGTACGGGAGCCAGGGAACAGCATGGCAAACGGGAAGTTCGGGTCAACGCGGCCCCACAATTCACCGTTAATAAAGTTGCCCAGACGCCCGGCACCAAGACCAAACGGAATGAGTGGTGCAATAAAATCAGAGACCTGgaaga                                                                                                                              <  1:4176298/150‑1 (MQ=255)
                    atCTTCTGTACGGGAGCCAGGGAACAGCATGGCAAACGGGAAGTTCGGGTCAACGCGGCCCCACAATTCACCGTTAATAAAGTTGCCCAGACGCCCGGCACCAAGACCAAACGGAATGAGTGGTGCAATAAAATCAGAGACCTGgaagaa                                                                                                                             >  1:4460457/1‑150 (MQ=255)
                        tctGTACGGGAGCCAGGGAACAGCATGGCAAACGGGAAGTTCGGGTCAACGCGGCCCCACAATTCACCGTTAATAAAGTTGCCCAGACGCCCGGCACCAAGACCAAACGGAATGAGTGGTGCAATAAAATCAGAGACCTGGAAGAAGGaa                                                                                                                         <  1:4203341/150‑1 (MQ=255)
                        tctGTACGGGAGCCAGGGAACAGCATGGCAAACGGGAAGTTCGGGTCAACGCGGCCCCACAATTCACCGTTAATAAAGTTGCCCAGACGCCCGGCACCAAGACCAAACGGAATGAGTGGTGCAATAAAATCAGAGACCTGGAAGAAGGaa                                                                                                                         <  1:3035220/150‑1 (MQ=255)
                         ctGTACGGGAGCCAGGGAACAGCATGGCAAACGGGAAGTTCGGGTCAACGCGGCCCCACAATTCACCGTTAATAAAGTTGCCCAGACGCCCGGCACCAAGACCAAACGGAATGAGTGGTGCAATAAAATCAGAGACCTGGAAGAAGGacc                                                                                                                        >  2:1726798/1‑148 (MQ=255)
                         ctGTACGGGAGCCAGGGAACAGCATGGCAAACGGGAAGTTCGGGTCAACGCGGCCCCACAATTCACCGTTAATAAAGTTGCCCAGACGCCCGGCACCAAGACCAAACGGAATGAGTGGTGCAATAAAATCAGAGACCTGGAAGAAGGAAc                                                                                                                        <  1:366552/150‑1 (MQ=255)
                         ctGTACGGGAGCCAGGGAACAGCATGGCAAACGGGAAGTTCGGGTCAACGCGGCCCCACAATTCACCGTTAATAAAGTTGCCCAGACGCCCGGCACCAAGACCAAACGGAATGAGTGGTGCAATAAAATCAGAGACCTGGAAGAAGGAAc                                                                                                                        <  2:1043132/150‑1 (MQ=255)
                           gTACGGGAGCCAGGGAACAGCATGGCAAACGGGAAGTTCGGGTCAACGCGGCCCCACAATTCACCGTTAATAAAGTTGCCCAGACGCCCGGCACCAAGACCAAACGGAATGAGTGGTGCAATAAAATCAGAGACCTGGAAGAAGGAACGt                                                                                                                      >  2:1940341/1‑150 (MQ=255)
                            tACGGGAGCCAGGGAACAGCATGGCAAACGGGAAGTTCGGGTCAACGCGGCCCCACAATTCACCGTTAATAAAGTTGCCCAGACGCCCGGCACCAAGACCAAACGGAATGAGTGGTGCAATAAAATCAGAGACCTGGAAGAAGGAACGtt                                                                                                                     >  2:1407480/1‑150 (MQ=255)
                             aCGGGAGCCAGGGAACAGCATGGCAAACGGGAAGTTCGGGTCAACGCGGCCCCACAATTCACCGTTAATAAAGTTGCCCAGACGCCCGGCACCAAGACCAAACGGAATGAGTGGTGCAATAAAATCAGAGACCTGGAAGAAGGAACGttt                                                                                                                    <  2:636885/150‑1 (MQ=255)
                              cGGGAGCCAGGGAACAGCATGGCAAACGGGAAGTTCGGGTCAACGCGGCCCCACAATTCACCGTTAATAAAGTTGCCCAGACGCCCGGCACCAAGACCAAACGGAATGAGTGGTGCAATAAAATCAGAGACCTGGAAGAAGGAACGTTTa                                                                                                                   >  2:4931502/1‑150 (MQ=255)
                              cGGGAGCCAGGGAACAGCATGGCAAACGGGAAGTTCGGGTCAACGCGGCCCCACAATTCACCGTTAATAAAGTTGCCCAGACGCCCGGCACCAAGACCAAACGGAATGAGTGGTGCAATAAAATCAGAGACCTGGAAGAAGGAACGTTTa                                                                                                                   <  1:830106/150‑1 (MQ=255)
                              cGGGAGCCAGGGAACAGCATGGCAAACGGGAAGTTCGGGTCAACGCGGCCCCACAATTCACCGTTAATAAAGTTGCCCAGACGCCCGGCACCAAGACCAAACGGAATGAGTGGTGCAATAAAATCAGAGACCTGGAAGAAGGAACGTTTa                                                                                                                   <  2:2965166/150‑1 (MQ=255)
                               gggAGCCAGGGAACAGCATGGCAAACGGGAAGTTCGGGTCAACGCGGCCCCACAATTCACCGTTAATAAAGTTGCCCAGACGCCCGGCACCAAGACCAAACGGAATGAGTGGTGCGATAAAATCAGAGACCTGGAAGAAGGAACGTTTAg                                                                                                                  >  2:761671/1‑150 (MQ=255)
                                ggAGCCAGGGAACAGCATGGCAAACGGGAAGTTCGGGTCAACGCGGCCCCACAATTCACCGTTAATAAAGTTGCCCAGACGCCCGGCACCAAGACCAAACGGAATGAGTGGTGCAATAAAATCAGAGACCTGGAAGAAGGAACGTTTAGt                                                                                                                 <  1:1711576/150‑1 (MQ=255)
                                 gAGCCAGGGAACAGCATGGCAAACGGGAAGTTCGGGTCAACGCGGCCCCACAATTCACCGTTAATAAAGTTGCCCAGACGCCCGGCACCAAGACCAAACGGAATGAGTGGTGCAATAAAATCAGAGACCTGGAAGAAGGAACGTTTAGTa                                                                                                                >  1:856101/1‑150 (MQ=255)
                                  aGCCAGGGAACAGCATGGCAAACGGGAAGTTCGGGTCAACGCGGCCCCACAATTCACCGTTAATAAAGTTGCCCAGACGCCCGGCACCAAGACCAAACGGAATGAGTGGTGCAATAAAATCAGAGACCTGGAAGAAGGAACGTTTAGTAc                                                                                                               <  2:4862986/150‑1 (MQ=255)
                                  aGCCAGGGAACAGCATGGCAAACGGGAAGTTCGGGTCAACGCGGCCCCACAATTCACCGTTAATAAAGTTGCCCAGACGCCCGGCACCAAGACCAAACGGAATGAGTGGTGCAATAAAATCAGAGACCTGGAAGAAGGAACGTTTAGTAc                                                                                                               <  1:1352028/150‑1 (MQ=255)
                                     cAGGGAACAGCATGGCAAACGGGAAGTTCGGGTCAACGCGGCCCCACAATTCACCGTTAATAAAGTTGCCCAGACGCCCGGCACCAAGACCAAACGGAATGAGTGGTGCAATAAAATCAGAGACCTGGAAGAAGGAACGTTTAGTAcggc                                                                                                            >  2:4448769/1‑150 (MQ=255)
                                     cAGGGAACAGCATGGCAAACGGGAAGTTCGGGTCAACGCGGCCCCACAATTCACCGTTAATAAAGTTGCCCAGACGCCCGGCACCAAGACCAAACGGAATGAGTGGTGCAATAAAATCAGAGACCTGGAAGAAGGAACGTTTAGTAcggc                                                                                                            <  2:3443951/150‑1 (MQ=255)
                                     cAGGGAACAGCATGGCAAACGGGAAGTTCGGGTCAACGCGGCCCCACAATTCACCGTTAATAAAGTTGCCCAGACGCCCGGCACCAAGACCAAACGGAATGAGTGGTGCAATAAAATCAGAGACCTGGAAGAAGGAACGTTTAGTAcggc                                                                                                            >  1:2229282/1‑150 (MQ=255)
                                       gggAACAGCATGGCAAACGGGAAGTTCGGGTCAACGCGGCCCCACAATTCACCGTTAATAAAGTTGCCCAGACGCCCGGCACCAAGACCAAACGGAATGAGTGGTGCAATAAAATCAGAGACCTGGAAGAAGGAACGTTTAGTAcggcgg                                                                                                          <  1:2596608/150‑1 (MQ=255)
                                                  ggCAAACGGGAAGTTCGGGTCAACGCGGCCCCACAATTCACCGTTAATAAAGTTGCCCAGACGCCCGGCACCAAGACCAAACGGAATGAGTGGTGCAATAAAATCAGAGACCTGGAAGAAGGAACGTTTAGTACGGCGGGCGAAGATAat                                                                                               <  2:2810952/150‑1 (MQ=255)
                                                     aaaCGGGAAGTTCGGGTCAACGCGGCCCCACAATTCACCGTTAATAAAGTTGCCCAGACGCCCGGCACCAAGACCAAACGGAATGAGTGGTGCAATAAAATCAGAGACCTGGAAGAAGGAACGTTTAGTACGGCGGGCGAAGATAatcat                                                                                            <  2:2869837/150‑1 (MQ=255)
                                                      aaCGGGAAGTTCGGGTCAACGCGGCCCCACAATTCACCGTTAATAAAGTTGCCCAGACGCCCGGCACCAAGACCAAACGGAATGAGTGGTGCAATAAAATCAGAGACCTGGAAGAAGGAACGTTTAGTACGGCGGGCGAAGATAatcatc                                                                                           >  2:4557139/1‑150 (MQ=255)
                                                      aaCGGGAAGTTCGGGTCAACGCGGCCCCACAATTCACCGTTAATAAAGTTGCCCAGACGCCCGGCACCAAGACCAAACGGAATGAGTGGTGCAATAAAATCAGAGACCTGGAAGAAGGAACGTTTAGTACGGCGGGCGAAGATAatcatc                                                                                           >  2:4557126/1‑150 (MQ=255)
                                                       ccGGGAAGGTCGGGTCAACGCGGCCCCACAATTCACCGTTAATAAAGGTGCCCAGACGCCCGGCACCAAGACCAAACGGAATGAGTGGTGCAATAAAATCAGAGACCTGGAAGAAGGAACGTTTAGTACGGCGGGCGAAGATAAtcatca                                                                                          <  2:4899032/149‑1 (MQ=255)
                                                       aCGGGAAGTTCGGGTCAACGCGGCCCCACAATTCACCGTTAATAAAGTTGCCCAAACGCCCGGCACCAAGACCAAACGGAATGAGTGGTGCAATAAAATCAGAGACCTGGAAGAAGGAACGTTTAGTACGGCGGGCGAAGATAAtcatca                                                                                          >  1:4805432/1‑150 (MQ=255)
                                                            aaGTTCGGGTCAACGCGGCCCCACAATTCACCGTTAATAAAGTTGCCCAGACGCCCGGCACCAAGACCAAACGGAATGAGTGGTGCAATAAAATCAGAGACCTGGAAGAAGGAACGTTTAGTACGGCGGGCGAAGATAATCATCACCACg                                                                                     >  2:3015492/1‑150 (MQ=255)
                                                             aGTTCGGGTCAACGCGGCCCCACAATTCACCGTTAATAAAGTTGCCCAGACGCCCGGCACCAAGACCAAACGGAATGAGTGGTGCAATAAAATCAGAGACCTGGAAGAAGGAACGTTTAGTACGGCGGGCGAAGATAATCATCACCACGa                                                                                    >  2:3855134/1‑150 (MQ=255)
                                                                tCGGGTCAACGCGGCCCCACAATTCACCGTTAATAAAGTTGCCCAGACGCCCGGCACCAAGACCAAACGGAATGAGTGGTGCAATAAAATCAGAGACCTGGAAGAAGGAACGTTTAGTACGGCGGGCGAAGATAATCATCACCACGATaa                                                                                 <  2:1350088/150‑1 (MQ=255)
                                                                 cGGGTCAACGCGGCCCCACAATTCACCGTTAATAAAGTTGCCCAGACGCCCGGCACCAAGACCAAACGGAATGAGTGGTGCAATAAAATCAGAGACCTGGAAGAAGGAACGTTTAGTACGGCGGGCGAAGATAATCATCACCACGATAAc                                                                                <  2:4919757/150‑1 (MQ=255)
                                                                  gggTCAACGCGGCCCCACAATTCACCGTTAATAAAGTTGCCCAGACGCCCGGCACCAAGACCAAACGGAATGAGTGGTGCAATAAAATCAGAGACCTGGAAGAAGGAACGTTTAGTACGGCGGGCGAAGATAATCATCACCACGATAACg                                                                               <  1:3015492/150‑1 (MQ=255)
                                                                  gggTCAACGCGGCCCCACAATTCACCGTTAATAAAGTTGCCCAGACGCCCGGCACCAAGACCAAACGGAATGAGTGGTGCAATAAAATCAGAGACCTGGAAGAAGGAACGTTTAGTACGGCGGGCGAAGATAATCATCACCACGATAACg                                                                               >  1:2044367/1‑150 (MQ=255)
                                                                  gggTCAACGCGGCCCCACAATTCACCGTTAATAAAGTTGCCCAGACGCCCGGCACCAAGACCAAACGGAATGAGTGGTGCAATAAAATCAGAGACCTGGAAGAAGGAACGTTTAGTACGGCGGGCGAAGATAATCATCACCACGATAACg                                                                               <  1:3864031/150‑1 (MQ=255)
                                                                        aCGCGGCCCCACAATTCACCGTTAATAAAGTTGCCCAGACGCCCGGCACCAAGACCAAACGGAATGAGTGGTGCGATAAAATCAGAGACCTGGAAGAAGGAACGTTTAGTACGGCGGGCGAAGATAATCATCACCACGATAACGCCAATc                                                                         <  1:4677927/150‑1 (MQ=255)
                                                                         cgcgGCCCCACAATTCACCGTTAATAAAGTTGCCCAGACGCCCGGCACCAAGACCAAACGGAATGAGTGGTGCAATAAAATCAGAGACCTGGAAGAAGGAACGTTTAGTACGGCGGGCGAAGATAATCATCACCACGATAACGCCAATCa                                                                        >  1:332256/1‑150 (MQ=255)
                                                                          gcgGCCCCACAATTCACCGTTAATAAAGTTGCCCAGACGCCCGGCACCAAGACCAAACGGAATGAGTGGTGCGATAAAATCAGAGACCTGGAAGAAGGAACGTTTAGTACGGCGGGCGAAGATAATCATCACCACGCTAACGCCAATCAg                                                                       <  2:5092042/150‑1 (MQ=255)
                                                                          gcgGCCCCACAATTCACCGTTAATAAAGTTGCCCAGACGCCCGGCACCAAGACCAAACGGAATGAGTGGTGCGATAAAATCAGAGACCTGGAAGAAGGAACGTTTAGTACGGCGGGCGAAGATAATCATCACCACGATAACGCCAATCAg                                                                       <  2:150108/150‑1 (MQ=255)
                                                                            ggCCCCACAATTCACCGTTAATAAAGTTGCCCAGACGCCCGGCACCAAGACCAAACGGAATGAGTGGTGCAATAAAATCAGAGACCTGGAAGAAGGAACGTTTAGTACGGCGGGCGAAGATAATCATCACCACGATAACGCCAATCAGgc                                                                     <  1:1904591/150‑1 (MQ=255)
                                                                            ggCCCCACAATTCACCGTTAATAAAGTTGCCCAGACGCCCGGCACCAAGACCAAACGGAATGAGTGGTGCAATAAAATCAGAGACCTGGAAGAAGGAACGTTTAGTACGGCGGGCGAAGATAATCATCACCACGATAACGCCAATCAGgc                                                                     >  1:4085203/1‑150 (MQ=255)
                                                                               cccACAATTCACCGTTAATAAAGTTGCCCAGACGCCCGGCACCAAGACCAAACGGAATGAGTGGTGCAATAAAATCAGAGACCTGGAAGAAGGAACGTTTAGTACGGCGGGCGAAGATAATCATCACCACGATAACGCCAATCAGgccgc                                                                  >  2:1396646/1‑150 (MQ=255)
                                                                                  acaATTCACCGTTAATAAAGTTGCCCAGACGCCCGGCACCAAGACCAAACGGAATGAGTGGTGCAATAAAATCAGAGACCTGGAAGAAGGAACGTTTAGTACGGCGGGCGAAGATAATCATCACCACGATAACGCCAATCAGGCCGCCGt                                                               >  2:387731/1‑150 (MQ=255)
                                                                                   caATTCACCGTTAATAAAGTTGCCCAGACGCCCGGCACCAAGACCAAACGGAATGAGTGGTGCAATAAAATCAGAGACCTGGAAGAAGGAACGTTTAGTACGGCGGGCGAAGATAATCATCACCACGATAACGCCAATCAGGCCGCCGTg                                                              >  1:4605081/1‑150 (MQ=255)
                                                                                   caATCCACCGTTAATAAAGTTGCCCAGACGCCCGGCACCAAGACCAAACGGAATGAGTGGTGCAATAAAATCAGAGACCTGGAAGAAGGAACGTTTAGTACGGCGGGCGAAGATAATCATCACCACGATAACGCCAATCAGGCCGCCGTg                                                              <  1:3964648/150‑1 (MQ=255)
                                                                                           cGTTAATAAAGTTGCCCAGACGCCCGGCACCAAGACCAAACGGAATGAGTGGTGCAATAAAATCAGAGACCTGGAAGAAGGAACGTTTAGTACGGCGGGCGAAGATAATCATCACCACGATAACGCCAATCAGGCCGCCGTGGAAAGACa                                                      >  2:3298826/1‑150 (MQ=255)
                                                                                            gTTAATAAAGTTGCCCAGACGCCCGGCACCAAGACCAAACGGAATGAGTGGTGCAATAAAATCAGAGACCTGGAAGAAGGAACGTTTAGTACGGCGGGCGAAGATAATCATCACCACGATAACGCCAATCAGGCCGCCGTGGAAAGACAt                                                     >  2:3217678/1‑150 (MQ=255)
                                                                                               aataaAGTTGCCCAGACGCCCGGCACCAAGACCAAACGGAATGAGTGGTGCAATAAAATCAGAGACCTGGAAGAAGGAACGTTTAGTACGGCGGGCGAAGATAATCATCACCACGATAACGCCAATCAGGCCGCCGTGGAAAGACATgcc                                                  >  2:4720327/1‑150 (MQ=255)
                                                                                               aataaAGTTGCCCAGACGCCCGGCACCAAGACCAAACGGAATGAGTGGTGCAATAAAATCAGAGACCTGGAAGAAGGAACGTTTAGTACGGCGGGCGAAGATAATCATCACCACGATAACGCCAATCAGGCCGCCGTGGAAAGACATgcc                                                  >  2:5058472/1‑150 (MQ=255)
                                                                                                  aaaGTTGCCCAGACGCCCGGCACCAAGACCAAACGGAATGAGTGGTGCAATAAAATCAGAGACCTGGAAGAAGGAACGTTTAGTACGGCGGGCGAAGATAATCATCACCACGATAACGCCAATCAGGCCGCCGTGGAAAGACATgccgcc                                               <  1:3453765/150‑1 (MQ=255)
                                                                                                  aaaGTTGCCCAGACGCCCGGCACCAAGACCAAACGGAATGAGTGGTGCAATAAAATCAGAGACCTGGAAGAAGGAACGTTTAGTACGGCGGGCGAAGATAATCATCACCACGATAACGCCAATCAGGCCGCCGTGGAAAGACATgccgcc                                               >  2:2305174/1‑150 (MQ=255)
                                                                                                   aaGTTGCCCAGACGCCCGGCACCAAGACCAAACGGAATGAGTGGTGCAATAAAATCAGAGACCTGGAAGAAGGAACGTTTAGTACGGCGGGCGAAGATAATCATCACCACGATAACGCCAATCAGGCCGCCGTGGAAAGACATGccgccg                                              >  2:2483594/1‑150 (MQ=255)
                                                                                                    aGTTGCCCAGACGCCCGGCACCAAGACCAAACGGAATGAGTGGTGCGATAAAATCAGAGACCTGGAAGAAGGAACGTTTAGTACGGCGGGCGAAGATAATCATCACCACGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGt                                             >  1:2879406/1‑150 (MQ=255)
                                                                                                     gTTGCCCAGACGCCCGGCACCAAGACCAAACGGAATGAGTGGTGCAATAAAATCAGAGACCTGGAAGAAGGAACGTTTAGTACGGCGGGCGAAGATAATCATCACCACGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGTc                                            >  2:3917781/1‑150 (MQ=255)
                                                                                                     gTTGCCCAGACGCCCGGCACCAAGACCAAACGGAATGAGTGGTGCAATAAAATCAGAGACCTGGAAGAAGGAACGTTTAGTACGGCGGGCGAAGATAATAATCACCACGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGTc                                            >  1:2166838/1‑150 (MQ=255)
                                                                                                       tGCCCAGACGCCCGGCACCAAGACCAAACGGAATGAGTGGTGCAATATAATCAGAGACCTGGAAGAAGGAACGTTTAGTACGGCGGGCGAAGATAATCATCACCACGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGTccc                                          <  1:2737975/150‑1 (MQ=255)
                                                                                                          ccAGACGCCCGGCACCAAGACCAAACGGAATGAGTGGTGCAATAAAATCAGAGACCTGGAAGAAGGAACGTTTAGTACGGCGGGCGAAGATAATCATCACCACGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGTCCCAGa                                       >  1:2944848/1‑150 (MQ=255)
                                                                                                               cGCCCGGCACCAAGACCAAACGGAATGAGTGGTGCAATAAAATCAGAGACCTGGAAGAAGGAACGTTTAGTACGGCGGGCGAAGATAATCATCACCACGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGTCCCAGACACgg                                  >  2:930745/1‑150 (MQ=255)
                                                                                                               cGCCCGGCACCAAGACCAAACGGAATGAGTGGTGCAATAAAATCAGAGACCTGGAAGAAGGAACGTTTAGTACGGCGGGCGAAGATAATCATCACCACGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGTCCCAGACACgg                                  >  2:1744452/1‑150 (MQ=255)
                                                                                                               cGCCCGGCACCAAGACCAAACGGAATGAGTGGTGCAATAAAATCAGAGACCTGGAAGAAGGAACGTTTAGTACGGCGGGCGAAGATAATCATCACCACGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGTCCCAGACACgg                                  >  1:2701892/1‑150 (MQ=255)
                                                                                                                gCCCGGCACCAAGACCAAACGGAATGAGTGGTGCAATAAAATCAGAGACCTGGAAGAAGGAACGTTTAGTACGGCGGGCGAAGATAATCATCACCACGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGTCCCAGACACGGa                                 <  1:1049786/150‑1 (MQ=255)
                                                                                                                gCCCGGCACCAAGACCAAACGGAATGAGTGGTGCAATAAAATCAGAGACCTGGAAGAAGGAACGTTTAGTACGGCGGGCGAAGATAATCATCACCACGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGTCCCAGACACGGa                                 <  2:4470882/150‑1 (MQ=255)
                                                                                                                gCCCGGCACCAAGACCAAACGGAATGAGTGGTGCAATAAAATCAGAGACCTGGAAGAAGGAACGTTTAGTACGGCGGGCGAAGATAATCATCACCACGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGTCCCAGACACGGa                                 >  2:165600/1‑150 (MQ=255)
                                                                                                                  ccGGCACCAAGACCAAACGGAATGAGTGGTGCAATAAAATCAGAGACCTGGAAGAAGGAACGTTTAGTACGGCGGGCGAAGATAATCATCACCACGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGTCCCAGACACGGAAc                               >  1:4247142/1‑150 (MQ=255)
                                                                                                                   cGGCACCAAGACCAAACGGAATGAGTGGTGCAATAAAATCAGAGACCTGGAAGAAGGAACGTTTAGTACGGCGGGCGAAGATAATCATCACCACGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGTCCCAGACACGGAACa                              >  2:834571/1‑150 (MQ=255)
                                                                                                                   cGGCACCAAGACCAAACGGAATGAGTGGTGCAATAAAATCAGAGACCTGGAAGAAGGAACGTTTAGTACGGCGGGCGAAGATAATCATCACCACGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGTCCCAGACACGGAACa                              >  1:3159010/1‑150 (MQ=255)
                                                                                                                        ccAAGACCAAACGGAATGAGTGGTGCAATAAAATCAGAGACCTGGAAGAAGGAACGTTTAGTACGGCGGGCGAAGATAATCATCACCACGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGTCCCAGACACGGAACAGATAc                         >  1:692684/1‑150 (MQ=255)
                                                                                                                        ccAAGACCAAACGGAATGAGTGGTGCAATAAAATCAGAGACCTGGAAGAAGGAACGTTTAGTACGGCGGGCGAAGATAATCATCACCACGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGTCCCAGACACGGAACAGATAc                         >  1:4955288/1‑150 (MQ=255)
                                                                                                                         cAAGACCAAACGGAATGAGTGGTGCAATAAAATCAGAGACCTGGAAGAAGGAACGTTTAGTACGGCGGGCGAAGATAATCATCACCACGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGTCCCAGACACGGAACAGATACa                        <  1:3634887/150‑1 (MQ=255)
                                                                                                                              ccAAACGGAATGAGTGGTGCAATAAAATCAGAGACCTGGAAGAAGGAACGTTTAGTACGGCGGGCGAAGATAATCATCACCACGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGTCCCAGACACGGAACAGATACAGCGGa                   <  1:650294/150‑1 (MQ=255)
                                                                                                                               cAAACGGAATGAGTGGTGCAATAAAATCAGAGACCTGGAAGAAGGAACGTTTAGTACGGCGGGCGAAGATAATCATCACCACGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGTCCCAGACACGGAACAGATACAGCGGAt                  >  2:3614753/1‑150 (MQ=255)
                                                                                                                               cAAACGGAATGAGTGGTGCAATAAAATCAGAGACCTGGAAGAAGGAACGTTTAGTACGGCGGGCGAAGATAATCATCACCACGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGTCCCAGACACGGAACAGATACAGCGGAt                  >  1:5085346/1‑150 (MQ=255)
                                                                                                                                 aaCGGAATGAGTGGTGCAATAAAATCAGAGACCTGGAAGAAGGAACGTTTAGTACGGCGGGCGAAGATAATCATCACCACGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGTCCCAGACACGGAACAGATACAGCGGATCg                >  1:1585161/1‑150 (MQ=255)
                                                                                                                                    ggAATGAGTGGTGCAATAAAATCAGAGACCTGGAAGAAGGAACGTTTAGTACGGCGGGCGAAGATAATCATCACCACGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGTCCCAGACACGGAACAGATACAGCGGATCGGcc             >  2:4247704/1‑150 (MQ=255)
                                                                                                                                    ggAATGAGTGGTGCAATAAAATCAGAGACCTGGAAGAAGGAACGTTTAGTACGGCGGGCGAAGATAATCATCACCACGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGTCCCAGACACGGAACAGATACAGCGGATCGGcc             >  2:4247684/1‑150 (MQ=255)
                                                                                                                                    ggAATGAGTGGTGCAATAAAATCAGAGACCTGGAAGAAGGAACGTTTAGTACGGCGGGCGAAGATAATCATCACCACGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGTCCCAGACACGGAACAGATACAGCGGATCGGcc             >  2:5130328/1‑150 (MQ=255)
                                                                                                                                    ggAATGAGTGGTGCAATAAAATCAGAGACCTGGAAGAAGGAACGTTTAGTACGGCGGGCGAAGATAATCATCACCACGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGTCCCAGACACGGAACAGATACAGCGGATCGGcc             >  1:2897604/1‑150 (MQ=255)
                                                                                                                                     gAATGAGTGGTGCAATAAAATCAGAGACCTGGAAGAAGGAACGTTTAGTACGGCGGGCGAAGATAATCATCACCACGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGTCCCAGACACGGAACAGATACAGCGGATCGGCCa            >  1:1846785/1‑150 (MQ=255)
                                                                                                                                     gAATGAGTGGTGCAATAAAATCAGAGACCTGGAAGAAGGAACGTTTAGTACGGCGGGCGAAGATAATCATCACCACGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGTCCCAGACACGGAACAGATACAGCGGATCGGCCa            >  1:2329542/1‑150 (MQ=255)
                                                                                                                                        tGAGTGGTGCAATAAAATCAGAGACCTGGAAGAAGGAACGTTTAGTACGGCGGGCGAAGATAATCATCACCACGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGTCCCAGACACGGAACAGATACAGCGGATCGGCCATaa         <  1:4773535/150‑1 (MQ=255)
                                                                                                                                           gtggtgCAATAAAATCAGAGACCTGGAAGAAGGAACGTTTAGTACGGCGGGCGAAGATAATCATCACCACGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGTCCCAGACACGGAACAGATACAGCGGATCGGCCATAAACt      <  1:1623717/150‑1 (MQ=255)
                                                                                                                                            tggtgCAATAAAATCAGAGACCTGGAAGAAGGAACGTTTAGTACGGCGGGCGAAGATAATCATCACCACGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGTCCCAGACACGGAACAGATACAGCGGATCGGCCATAAACTg     <  1:1277591/150‑1 (MQ=255)
                                                                                                                                             ggtgCAATAAAATCAGAGACCTGGAAGAAGGAACGTTTAGTACGGCGGGCGAAGATAATCATCACCACGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGTCCCAGACACGGAACAGATACAGCGGATCGGCCATAAACTGc    <  1:4927674/150‑1 (MQ=255)
                                                                                                                                             ggtgCAATAAAATCAGAGACCTGGAAGAAGGAACGTTTAGTACGGCGGGCGAAGATAATCATCACCACGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGTCCCAGACACGGAACAGATACAGCGGATCGGCCATAAACTGc    >  2:4753517/1‑150 (MQ=255)
                                                                                                                                             ggtgCAATAAAATCAGAGACCTGGAAGAAGGAACGTTTAGTACGGCGGGCGAAGATAATCATCACCACGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGTCCCAGACACGGAACAGATACAGCGGATCGGCCATAAACTGc    >  1:4220418/1‑150 (MQ=255)
                                                                                                                                             ggtgCAATAAAATCAGAGACCTGGAAGAAGGAACGTTTAGTACGGCGGGCGAAGATAATCATCACCACGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGTCCCAGACACGGAACAGATACAGCGGATCGGCCATAAACTGc    <  2:2419498/150‑1 (MQ=255)
                                                                                                                                               tgCAATAAAATCAGAGACCTGGAAGAAGGAACGTTTAGTACGGCGGGCGAAGATAATCATCACCACGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGTCCCAGACACGGAACAGATACAGCGGATCGGCCATAAACTGCgg  >  1:957412/1‑150 (MQ=255)
                                                                                                                                                  |                                                                                                                                                  
TTGGTTTGCAGCAGCAAAATATCTTCTGTACGGGAGCCAGGGAACAGCATGGCAAACGGGAAGTTCGGGTCAACGCGGCCCCACAATTCACCGTTAATAAAGTTGCCCAGACGCCCGGCACCAAGACCAAACGGAATGAGTGGTGCGATAAAATCAGAGACCTGGAAGAAGGAACGTTTAGTACGGCGGGCGAAGATAATCATCACCACGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGTCCCAGACACGGAACAGATACAGCGGATCGGCCATAAACTGCGG  >  CP009273/2958858‑2959150

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑