Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,967,572 A→G 34.5% F601S (TTT→TCT)  aas ← fused 2‑acylglycerophospho‑ethanolamine acyl transferase/acyl‑acyl carrier protein synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,967,5720AG34.5% 362.4 / inf 290F601S (TTT→TCT) aasfused 2‑acylglycerophospho‑ethanolamine acyl transferase/acyl‑acyl carrier protein synthetase
Reads supporting (aligned to +/- strand):  ref base A (94/95);  new base G (53/46);  total (147/143)
Fisher's exact test for biased strand distribution p-value = 6.20e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.77e-01

Only 100 of 300 total aligned reads displayed.

TGGCATCGCTCTTAATCGCAGTGGCATGGACTTTATCTGGCGAAACACCAAGTGCCAGTTGTTCCACCATTTCCAGCGACACCATTTCGCCTGCAATTTTGGCAAAGCGTTTTGCGCGGCCCTGAATCTGCACAAAGCCCTGCTCGTCAAAACGCACAATATCGCCAGTGTCATACCAGCCGCGCTCCATTTCGCCGCGAACATTCTCGGCGGTGGGCACTTCCAGTACACCTGGCTTCTCCACCCGCAGATAGCCGTTCATTATGTTCGGCCCTTTCAGTTGCAGGCGTCCGCCCTCT  >  CP009273/2967423‑2967721
                                                                                                                                                     |                                                                                                                                                     
tGGCATCGCTCTTAATCGCAGTGGCATGGACTTTATCTGGCGAAACACCAAGTGCCAGTTGTTCCACCATTTCCAGCGACACCATTTCGCCTGCAATTTTGGCAAAGCGTTTTGCGCGGCCCTGAATCTGCACAAAGCCCTGCTCGTCaa                                                                                                                                                       <  1:2732588/150‑1 (MQ=255)
 ggCATCGCTCTTAATCGCAGTGGCATGGACTTTATCTGGCGAAACACCAAGTGCCAGTTGTTCCACCATTTCCAGCGACACCATTTCGCCTGCAATTTTGGCAAAGCGTTTTGCGCGGCCCTGAATCTGCACAAAGCCCTGCTCGTCaaa                                                                                                                                                      >  1:161589/1‑150 (MQ=255)
   cATCGCTCTTAATCGCAGTGGCATGGACTTTATCTGGCGAAACACCAAGTGCCAGTTGTTCCACCATTTCCAGCGACACCATTTCGCCTGCAATTTTGGCAAAGCGTTTTGCGCGGCCCTGAATCTGCACAAAGCCCTGCTCGTCAAAAc                                                                                                                                                    <  2:601420/150‑1 (MQ=255)
   cATCGCTCTTAATCGCAGTGGCATGGACTTTATCTGGCGAAACACCAAGTGCCAGTTGTTCCACCATTTCCAGCGACACCATTTCGCCTGCAATTTTGGCAAAGCGTTTTGCGCGGCCCTGAATCTGCACAAAGCCCTGCTCGTCAAAAc                                                                                                                                                    <  2:2617010/150‑1 (MQ=255)
   cATCGCTCTTAATCGCAGTGGCATGGACTTTATCTGGCGAAACACCAAGTGCCAGTTGTTCCACCATTTCCAGCGACACCATTTCGCCTGCAATTTTGGCAAAGCGTTTTGCGCGGCCCTGAATCTGCACAAAGCCCTGCTCGTCAAAAc                                                                                                                                                    <  2:4144546/150‑1 (MQ=255)
   cATCGCTCTTAATCGCAGTGGCATGGACTTTATCTGGCGAAACACCAAGTGCCAGTTGTTCCACCATTTCCAGCGACACCATTTCGCCTGAAATTTTGGCAAAGCGTTTTGCGCGGCCCTGAATCTGCACAAAGCCCTGCTCGTCAAAAc                                                                                                                                                    <  1:1379639/150‑1 (MQ=255)
    accGCTCTTAATCGCAGTGGCATGGACTTTATCTGGCGAAACACCAAGTGCCAGTTGTTCCACCATTTCCAGCGACACCATTTCGCCTGCAATTTTGGCAAAGCGTTTTGCGCGGCCCTGAATCTGCACAAAGCCCTGCTCGTCAAAACg                                                                                                                                                   >  1:3120681/3‑150 (MQ=255)
     tCGCTCTTAATCGCAGTGGCATGGACTTTATCTGGCGAAACACCAAGTGCCAGTTGTTCCACCATTTCCAGCGACACCATTTCGCCTGCAATTTTGGCAAAGCGTTTTGCGCGGCCCTGAATCTGCACAAAGCCCTGCTCGTCAAAACGc                                                                                                                                                  >  1:3379118/1‑150 (MQ=255)
      cGCTCTTAATCGCAGTGGCATGGACTTTATCTGGCGAAACACCAAGTGCCAGTTGTTCCACCATTTCCAGCGACACCATTTCGCCTGCAATTTTGGCAAAGCGTTTTGCGCGGCCCTGAATCTGCACAAAGCCCTGCTCGTCAGAACGca                                                                                                                                                 <  1:1235644/150‑1 (MQ=255)
       gCTCTTAATCGCAGTGGCATGGACTTTATCTGGCGAAACACCAAGTGCCAGTTGTTCCACCATTTCCAGCGACACCATTTCGCCTGCAATTTTGGCAAAGCGTTTTGCGCGGCCCTGAATCTGCACAAAGCCCTGCTCGTCAAAACGcac                                                                                                                                                >  1:2313154/1‑150 (MQ=255)
        ctctTAATCGCAGTGGCATGGACTTTATCTGGCGAAACACCAAGTGCCAGTTGTTCCACCATTTCCAGCGACACCATTTCGCCTGCAATTTTGGCAAAGCGTTTTGCGCGGCCCTGAATCTGCACAAAGCCCTGCTCGTCAGAACGcaca                                                                                                                                               >  2:920583/1‑150 (MQ=255)
             aaTCGCAGTGGCATGGACTTTATCTGGCGAAACACCAAGTGCCAGTTGTTCCACCATTTCCAGCGACACCATTTCGCCTGCAATTTTGGCAAAGCGTTTTGCGCGGCCCTGAATCTGCACAAAGCCCTGCTCGTCAAAACGCACAATATc                                                                                                                                          >  2:5124525/1‑150 (MQ=255)
              aTCGCAGTGGCATGGACTTTATCTGGCGAAACACCAAGTGCCAGTTGTTCCACCATTTCCAGCGACACCATTTCGCCTGCAATTTTGGCAAAGCGTTTTGCGCGGCCCTGAATCTGCACAAAGCCCTGCTCGTCAAAACGCACAATATCg                                                                                                                                         <  2:4189827/150‑1 (MQ=255)
                 gCAGTGGCATGGACTTTATCTGGCGAAACACCAAGTGCCAGTTGTTCCACCATTTCCAGCGACACCATTTCGCCTGCAATTTTGGCAAAGCGTTTTGCGCGGCCCTGAATCTGCACAAAGCCCTGCTCGTCAAAACGCACAATATCGCCa                                                                                                                                      >  1:65003/1‑150 (MQ=255)
                  cAGTGGCATGGACTTTATCTGGCGAAACACCAAGTGCCAGTTGTTCCACCATTTCCAGCGACACCATTTCGCCTGCAATTTTGGCAAAGCGTTTTGCGCGGCCCTGAATCTGCACAAAGCCCTGCTCGTCAAAACGCACAATATCGCCAg                                                                                                                                     >  1:2093691/1‑150 (MQ=255)
                   aGTGGCATGGACTTTATCTGGCGAAACACCAAGTGCCAGTTGTTCCACCATTTCCAGCGACACCATTTCGCCTGCAATTTTGGCAAAGCGTTTTGCGCGGCCCTGAATCTGCACAAAGCCCTGCTCGTCAGAACGCACAATATCGCCAgt                                                                                                                                    <  2:2293427/150‑1 (MQ=255)
                    gTGGCATGGACTTTATCTGGCGAAACACCAAGTGCCAGTTGTTCCACCATTTCCAGCGACACCATTTCGCCTGCAATTTTGGCAAAGCGTTTTGCGCGGCCCTGAATCTGCACAAAGCCCTGCTCGTCAGAACGCACAATATCGCCAgtg                                                                                                                                   <  2:3418670/150‑1 (MQ=255)
                      ggCATGGACTTTATCTGGCGAAACACCAAGTGCCAGTTGTTCCACCATTTCCAGCGACACCATTTCGCCTGCAATTTTGGCAAAGCGTTTTGCGCGGCCCTGAATCTGCACAAAGCCCTGCTCGTCAAAACGCACAATATCGCCAGTGTc                                                                                                                                 <  1:1184226/150‑1 (MQ=255)
                       gCATGGACTTTATCTGGCGAAACACCAAGTGCCAGTTGTTCCACCATTTCCAGCGACACCATTTCGCCTGCAATTTTGGCAAAGCGTTTTGCGCGGCCCTGAATCTGCACAAAGCCCTGCTCGTCAAAACGCACAATATCGCCAGTGTCa                                                                                                                                >  1:3851299/1‑150 (MQ=255)
                       gCATGGACTTTATCTGGCGAAACACCAAGTGCCAGTTGTTCCACCATTTCCAGCGACACCATTTCGCCTGCAATTTTGGCAAAGCGTTTTGCGCGGCCCTGAATCTGCACAAAGCCCTGCTCGTCAAAACGCACAATATCGCCAGTGTCa                                                                                                                                <  1:787733/150‑1 (MQ=255)
                        cATGGACTTTATCTGGCGAAACACCAAGTGCCAGTTGTTCCACCATTTCCAGCGACACCATTTCGCCTGCAATTTTGGCAAAGCGTTTTGCGCGGCCCTGAATCTGCACAAAGCCCTGCTCGTCAAAACGCACAATATCGCCAGTGTCAt                                                                                                                               <  1:1038223/150‑1 (MQ=255)
                        cATGGACTTTATCTGGCGAAACACCAAGTGCCAGTTGTTCCACCATTTCCAGCGACACCATTTCGCCTGCAATTTTGGCAAAGCGTTTTGCGCGGCCCTGAATCTGCACAAAGCCCTGCTCGTCAAAACGCACAATATCGCCAGTGTCAt                                                                                                                               >  2:4813199/1‑150 (MQ=255)
                           ggACTTTATCTGGCGAAACACCAAGTGCCAGTTGTTCCACCATTTCCAGCGACACCATTTCGCCTGCAATTTTGGCAAAGCGTTTTGCGCGGCCCTGAATCTGCACAAAGCCCTGCTCGTCAGAACGCACAATATCGCCAGTGTCATAcc                                                                                                                            <  2:2748450/150‑1 (MQ=255)
                             aCTTTATCTGGCGAAACACCAAGTGCCAGTTGTTCCACCATTTCCAGCGACACCATTTCGCCTGCAATTTTGGCAAAGCGTTTTGCGCGGCCCTGAATCTGCACAACGCCCTGCTCGTCAGAACGCACAATATCGCCAGTGTCATACCAg                                                                                                                          <  2:1614445/150‑1 (MQ=255)
                              cTTTATCTGGCGAAACACCAAGTGCCAGTTGTTCCACCATTTCCAGCGACACCATTTCGCCTGCAATTTTGGCAAAGCGTTTTGCGCGGCCCTGAATCTGCACAAAGCCCTGCTCGTCAAAACGCACAATATCGCCAGTGTCATACCAGc                                                                                                                         >  2:5096659/1‑150 (MQ=255)
                                  aTCTGGCGAAACACCAAGTGCCAGTTGTTCCACCATTTCCAGCGACACCATTTCGCCTGCAATTTTGGCAAAGCGTTTTGCGCGGCCCTGAATCTGCACAAAGCCCTGCTCGTCAAAACGCACAATATCGCCAGTGTCATACCAGCcgcg                                                                                                                     <  1:1361216/150‑1 (MQ=255)
                                     tGGCGAAACACCAAGTGCCAGTTGTTCCACCATTTCCAGCGACACCATTTCGCCTGCAATTTTGGCAAAGCGTTTTGCGCGGCCCTGAATCTGCACAAAGCCCTGCTCGTCAGAACGCACAATATCGCCAGTGTCATACCAGCCGCGCTc                                                                                                                  <  2:4658383/150‑1 (MQ=255)
                                     tGGCGAAACACCAAGTGCCAGTTGTTCCACCATTTCCAGCGACACCATTTCGCCTGCAATTTTGGCAAAGCGTTTTGCGCGGCCCTGAATCTGCACAAAGCCCTGCTCGTCAAAACGCACAATATCGCCAGTGTCATACCAGCCGCGCTc                                                                                                                  >  1:1935012/1‑150 (MQ=255)
                                      ggCGAAACACCAAGTGCCAGTTGTTCCACCATTTCCAGCGACACCATTTCGCCTGCAATTTTGGCAAAGCGTTTTGCGCGGCCCTGAATCTGCACAAAGCCCTGCTCGTCAAAACGCACAATATCGCCAGTGTCATACCAGCCGCGCTcc                                                                                                                 <  2:4390361/150‑1 (MQ=255)
                                      ggCGAAACACCAAGTGCCAGTTGTTCCACCATTTCCAGCGACACCATTTCGCCTGCAATTTTGGCAAAGCGTTTTGCGCGGCCCTGAATCTGCACAAAGCCCTGCTCGTCAAAACGCACAATATCGCCAGTGTCATACCAGCCGCGCTcc                                                                                                                 <  1:712738/150‑1 (MQ=255)
                                        cGAAACACCAAGTGCCAGTTGTTCCACCATTTCCAGCGACACCATTTCGCCTGCAATTTTGGCAAAGCGTTTTGCGCGGCCCTGAATCTGCACAAAGCCCTGCTCGTCAAAACGCACAATATCGCCAGTGTCATACCAGCCGCGCTCCAt                                                                                                               <  2:4016699/150‑1 (MQ=255)
                                         gAAACACCAAGTGCCAGTTGTTCCACCATTTCCAGCGACACCATTTCGCCTGCAATTTTGGCAAAGCGTTTTGCGCGGCCCTGAATCTGCACAAAGCCCTGCTCGTCAAAACGCACAATATCGCCAGTGTCATACCAGCCGCGCTCCAtt                                                                                                              <  2:2100015/150‑1 (MQ=255)
                                           aaCACCAAGTGCCAGTTGTTCCACCATTTCCAGCGACACCATTTCGCCTGCAATTTTGGCAAAGCGTTTTGCGCGGCCCTGAATCTGCACAAAGCCCTGCTCGTCAAAACGCACAATATCGCCAGTGTCATACCAGCCGCGCTCCATTTc                                                                                                            <  2:1734950/150‑1 (MQ=255)
                                           aaCACCAAGTGCCAGTTGTTCCACCATTTCCAGCGACACCATTTCGCCTGCAATTTTGGCAAAGCGTTTTGCGCGGCCCTGAATCTGCACAAAGCCCTGCTCGTCAAAACGCACAATATCGCCAGTGTCATACCAGCCGCGCTCCATTTc                                                                                                            <  2:1284880/150‑1 (MQ=255)
                                            acacCAAGTGCCAGTTGTTCCACCATTTCCAGCGACACCATTTCGCCTGCAATTTTGGCAAAGCGTTTTGCGCGGCCCTGAATCTGCACAAAGCCCTGCTCGTCAAAACGCACAATATCGCCAGTGTCATACCAGCCGCGCTCCATTTcg                                                                                                           <  2:3102734/150‑1 (MQ=255)
                                             cacCAAGTGCCAGTTGTTCCACCATTTCCAGCGACACCATTTCGCCTGCAATTTTGGCAAAGCGTTTTGCGCGGCCCTGAATCTGCACAAAGCCCTGCTCGTCAGAACGCACAATATCGCCAGTGTCATACCAGCCGCGCTCCATTTcgc                                                                                                          >  1:2000400/1‑150 (MQ=255)
                                             cacCAAGTGCCAGTTGTTCCACCATTTCCAGCGACACCATTTCGCCTGCAATTTTGGCAAAGCGTTTTGCGCGGCCCTGAATCTGCACAAAGCCCTGCTCGTCAAAACGCACAATATCGCCAGTGTCATACCAGCCGCGCTCCATTTcgc                                                                                                          >  2:1139382/1‑150 (MQ=255)
                                              acCAAGTGCCAGTTGTTCCACCATTTCCAGCGACACCATTTCGCCTGCAATTTTGGCAAAGCGTTTTGCGCGGCCCTGAATCTGCACAAAGCCCTGCTCGTCAGAACGCACAATATCGCCAGTGTCATACCAGCCGCGCTCCATTTcgcc                                                                                                         >  1:4656944/1‑150 (MQ=255)
                                               ccAAGTGCCAGTTGTTCCACCATTTCCAGCGACACCATTTCGCCTGCAATTTTGGCAAAGCGTTTTGCGCGGCCCTGAATCTGCACAAAGCCCTGCTCGTCAAAACGCACAATATCGCCAGTGTCATACCAGCCGCGCTCCATTTcgccg                                                                                                        >  1:1515465/1‑150 (MQ=255)
                                               ccAAGTGCCAGTTGTTCCACCATTTCCAGCGACACCATTTCGCCTGCAATTTTGGCAAAGCGTTTTGCGCGGCCCTGAATCTGCACAAAGCCCTGCTCGTCAAAACGCACAATATCGCCAGTGTCATACCAGCCGCGCTCCATTTcgccg                                                                                                        <  2:3330671/150‑1 (MQ=255)
                                                cAAGTGCCAGTTGTTCCACCATTTCCAGCGACACCATTTCGCCTGCAATTTTGGCAAAGCGTTTTGCGCGGCCCTGAATCTGCACAAAGCCCTGCTCGTCAGAACGCACAATATCGCCAGTGTCATACCAGCCGCGCTCCATTTcgccgc                                                                                                       >  2:4265495/1‑150 (MQ=255)
                                                 aaGTGCCAGTTGTTCCACCATTTCCAGCGACACCATTTCGCCTGCAATTTTGGCAAAGCGTTTTGCGCGGCCCTGAATCTGCACAAAGCCCTGCTCGTCAGAACGCACAATATCGCCAGTGTCATACCAGCCGCGCTCCATTTCGCcgcg                                                                                                      <  2:2861776/150‑1 (MQ=255)
                                                 aaGTGCCAGTTGTTCCACCATTTCCAGCGACACCATTTCGCCTGCAATTTTGGCAAAGCGTTTTGCGCGGCCCTGAATCTGCACAAAGCCCTGCTCGTCAAAACGCACAATATCGCCAGTGTCATACCAGCCGCGCTCCATTTCGCcgcg                                                                                                      >  1:1851225/1‑150 (MQ=255)
                                                  aGTGCCAGTTGTTCCACCATTTCCAGCGACACCATTTCGCCTGCAATTTTGGCAAAGCGTTTTGCGCGGCCCTGAATCTGCACAAAGCCCTGCTCGTCAAAACGCACAATATCGCCAGTGTCATACCAGCCGCGCTCCATTTCGCCGCGa                                                                                                     >  1:112027/1‑150 (MQ=255)
                                                     gCCAGTTGTTCCACCATTTCCAGCGACACCATTTCGCCTGCAATTTTGGCAAAGCGTTTTGCGCGGCCCTGAATCTGCACAAAGCCCTGCTCGTCAGAACGCACAATATCGCCAGTGTCATACCAGCCGCGCTCCATTTCGCCGCGAACa                                                                                                  >  2:179853/1‑150 (MQ=255)
                                                     gCCAGTTGTTCCACCATTTCCAGCGACACCATTTCGCCTGCAATTTTGGCAAAGCGTTTTGCGCGGCCCTGAATCTGCACAAAGCCCTGCTCGTCAAAACGCACAATATCGCCAGTGTCATACCAGCCGCGCTCCATTTCGCCGCGAACa                                                                                                  >  1:2236768/1‑150 (MQ=255)
                                                      ccAGTTGTTCCACCATTTCCAGCGACACCATTTCGCCTGCAATTTTGGCAAAGCGTTTTGCGCGGCCCTGAATCTGCACAAAGCCCTGCTCGTCAGAACGCACAATATCGCCAGTGTCATACCAGCCGCGCTCCATTTCGCCGCGAACAt                                                                                                 <  2:4064774/150‑1 (MQ=255)
                                                      ccAGTTGTTCCACCATTTCCAGCGACACCATTTCGCCTGCAATTTTGGCAAAGCGTTTTGCGCGGCCCTGAATCTGCACAAAGCCCTGCTCGTCAAAACGCACAATATCGCCAGTGTCATACCAGCCGCGCTCCATTTCGCCGCGAACAt                                                                                                 <  2:3286104/150‑1 (MQ=255)
                                                         gttgttCCACCATTTCCAGCGACACCATTTCGCCTGCAATTTTGGCAAAGCGTTTTGCGCGGCCCTGAATCTGCACAAAGCCCTGCTCGTCAAAACGCACAATATCGCCAGTGTCATACCAGCCGCGCTCCATTTCGCCGCGAACATtct                                                                                              <  1:5108746/150‑1 (MQ=255)
                                                          ttgttCCACCATTTCCAGCGACACCATTTCGCCTGCAATTTTGGCAAAGCGTTTTGCGCGGCCCTGAATCTGCACAAAGCCCTGCTCGTCAGAACGCACAATATCGCCAGTGTCATACCAGCCGCGCTCCATTTCGCCGCGAACATtctc                                                                                             >  1:4841588/1‑150 (MQ=255)
                                                           tgttCCACCATTTCCAGCGACACCATTTCGCCTGCAATTTTGGCAAAGCGTTTTGCGCGGCCCTGAATCTGCACAAAGCCCTGCTCGTCAAAACGCACAATATCGCCAGTGTCATACCAGCCGCGCTCCATTTCGCCGCGAACATTCTcg                                                                                            >  1:4721426/1‑150 (MQ=255)
                                                             ttCCACCATTTCCAGCGACACCATTTCGCCTGCAATTTTGGCAAAGCGTTTTGCGCGGCCCTGAATCTGCACAAAGCCCTGCTCGTCAGAACGCACAATATCGCCAGTGTCATACCAGCCGCGCTCCATTTCGCCGCGAACATTCTcggc                                                                                          <  2:3753499/150‑1 (MQ=255)
                                                             ttCCACCATTTCCAGCGACACCATTTCGCCTGCAATTTTGGCAAAGCGTTTTGCGCGGCCCTGAATCTGCACAAAGCCCTGCTCGTCAAAACGCACAATATCGCCAGTGTCATACCAGCCGCGCTCCATTTCGCCGCGAACATTCTcggc                                                                                          >  2:4628291/1‑150 (MQ=255)
                                                              tCCACCATTTCCAGCGACACCATTTCGCCTGCAATTTTGGCAAAGCGTTTTGCGCGGCCCTGAATCTGCACAAAGCCCTGCTCGTCAGAACGCACAATATCGCCAGTGTCATACCAGCCGCGCTCCATTTCGCCGCGAACATTCTcggcg                                                                                         >  1:216482/1‑150 (MQ=255)
                                                              tCCACCATTTCCAGCGACACCATTTCGCCTGCAATTTTGGCAAAGCGTTTTGCGCGGCCCTGAATCTGCACAAAGCCCTGCTCGTCAGAACGCACAATATCGCCAGTGTCATACCAGCCGCGCTCCATTTCGCCGCGAACATTCTcggcg                                                                                         <  2:2739856/150‑1 (MQ=255)
                                                              tCCACCATTTCCAGCGACACCATTTCGCCTGCAATTTTGGCAAAGCGTTTTGCGCGGCCCTGAATCTGCACAAAGCCCTGCTCGTCAGAACGCACAATATCGCCAGTGTCATACCAGCCGCGCTCCATTTCGCCGCGAACATTCTcggcg                                                                                         >  1:216542/1‑150 (MQ=255)
                                                               ccaccaTATCCAGCGACACCATTTCGCCTGCAATTTTGGCAAAGCGTTTTGCGCGGCCCTGAATCTGCACAAAGCCCTGCTCGTCAAAACGCACAATATCGCCAGTGTCATACCAGCCGCGCTCCATTTCGCCGCGAACATTCTcggcgg                                                                                        >  2:3220654/1‑150 (MQ=255)
                                                                  ccaTTTCCAGCGACACCATTTCGCCTGCAATTTTGGCAAAGCGTTTTGCGCGGCCCTGAATCTGCACAAAGCCCTGCTCGTCAAAACGCACAATATCGCCAGTGTCATACCAGCCGCGCTCCATTTCGCCGCGAACATTCTCGGCGGTgg                                                                                     <  2:3615867/150‑1 (MQ=255)
                                                                   caTTTCCAGCGACACCATTTCGCCTGCAATTTTGGCAAAGCGTTTTGCGCGGCCCTGAATCTGCACAAAGCCCTGCTCGTCAAAACGCACAATATCGCCAGTGTCATACCAGCCGCGCTCCATTTCGCCGCGAACATTCTCGGCGGTggg                                                                                    >  1:113200/1‑150 (MQ=255)
                                                                         cAGCGACACCATTTCGCCTGCAATTTTGGCAAAGCGTTTTGCGCGGCCCTGAATCTGCACAAAGCCCTGCTCGTCAGAACGCACAATATCGCCAGTGTCATACCAGCCGCGCTCCATTTCGCCGCGAACAATCTCGGCGGTGGGCACTTc                                                                              >  1:2823304/1‑150 (MQ=255)
                                                                           gCGACACCATTTCGCCTGCAATTTTGGCAAAGCGTTTTGCGCGGCCCTGAATCTGCACAAAGCCCTGCTCGTCAAAACGCACAATATCGCCAGTGTCATACCAGCCGCGCTCCATTTCGCCGCGAACATTCTCGGCGGTGGGCACTTCCa                                                                            <  2:2033937/150‑1 (MQ=255)
                                                                            cGACACCATTTCGCCTGCAATTTTGGCAAAGCGTTTTGCGCGGCCCTGAATCTGCACAAAGCCCTGCTCGTCAAAACGCACAATATCGCCAGTGTCATACCAGCCGCGCTCCATTTCGCCGCGAACATTCTCGGCGGTGGGCACTTCCAg                                                                           >  1:3796321/1‑150 (MQ=255)
                                                                             gACACCATTTCGCCTGCAATTTTGGCAAAGCGTTTTGCGCGGCCCTGAATCTGCACAAAGCCCTGCTCGTCAGAACGCACAATATCGCCAGTGTCATACCAGCCGCGCTCCATTTCGCCGCGAACATTCTCGGCGGTGGGCACTTCCAGt                                                                          <  1:1146623/150‑1 (MQ=255)
                                                                              acacCATTTCGCCTGCAATTTTGGCAAAGCGTTTTGCGCGGCCCTGAATCTGCACAAAGCCCTGCTCGTCAGAACGCACAATATCGCCAGTGTCATACCAGCCGCGCTCCATTTCGCCGCGAACATTCTCGGCGGTGGGCACTTCCAGTa                                                                         <  2:3591856/150‑1 (MQ=255)
                                                                               cacCATTTCGCCTGCAATTTTGGCAAAGCGTTTTGCGCGGCCCTGAATCTGCACAAAGCCCTGCTCGTCAAAACGCACAATAACGCCAGTGTCATACCAGCCGCGCTCCATTTCGCCGCGAACATTCTCGGCGGTGGGCACTTCCAGTac                                                                        >  1:574499/1‑150 (MQ=255)
                                                                                acCATTTCGCCTGCAATTTTGGCAAAGCGTTTTGCGCGGCCCTGAATCTGCACAAAGCCCTGCTCGTCAAAACGCACAATATCGCCAGTGTCATACCAGCCGCGCTCCATTTCGCCGCGAACATTCTCGGCGGTGGGCACTTCCAGTaca                                                                       >  1:3247505/1‑150 (MQ=255)
                                                                                acCATTTCGCCTGCAATTTTGGCAAAGCGTTTTGCGCGGCCCTGAATCTGCACAAAGCCCTGCTCGTCAAAACGCACAATATCGCCAGTGTCATACCAGCCGCGCTCCATTTCGCCGCGAACATTCTCGGCGGTGGGCACTTCCAGTaca                                                                       >  1:3491975/1‑150 (MQ=255)
                                                                                         ccTGCAATTTTGGCAAAGCGTTTTGCGCGGCCCTGAATCTGCACAAAGCCCTGCTCGTCAGAACGCACAATATCGCCAGTGTCATACCAGCCGCGCTCCATTTCGCCGCGAACATTCTCGGCGGTGGGCACTTCCAGTACACCTGGCTtc                                                              >  1:4308713/1‑150 (MQ=255)
                                                                                          cTGCAATTTTGGCAAAGCGTTTTGCGCGGCCCTGAATCTGCACAAAGCCCTGCTCGTCAGAACGCACAATATCGCCAGTGTCATACCAGCCGCGCTCCATTTCGCCGCGAACATTCTCGGCGGTGGGCACTTCCAGTACACCTGGCTtct                                                             >  2:3969097/1‑150 (MQ=255)
                                                                                             cAATTTTGGCAAAGCGTTTTGCGCGGCCCTGAATCTGCACAAAGCCCTGCTCGTCAAAACGCACAATATCGCCAGTGTCATACCAGCCGCGCTCCATTTCGCCGCGAACATTCTCGGCGGTGGGCACTTCCAGTACACCTGGCTTCTCCa                                                          >  1:534654/1‑150 (MQ=255)
                                                                                             cAATTTTGGCAAAGCGTTTTGCGCGGCCCTGAATCTGCACAAAGCCCTGCTCGTCAAAACGCACAATATCGCCAGTGTCATACCAGCCGCGCTCCATTTCGCCGCGAACATTCTCGGCGGTGGGCACTTCCAGTACACCTGGCTTCTCCa                                                          >  1:1765837/1‑150 (MQ=255)
                                                                                              aaTTTTGGCAAAGCGTTTTGCGCGGCCCTGAATCTGCACAAAGCCCTGCTCGTCAAAACGCACAATATCGCCAGTGTCATACCAGCCGCGCTCCATTTCGCCGCGAACATTCTCGGCGGTGGGCACTTCCAGTACACCTGGCTTCTCCAc                                                         >  2:693907/1‑150 (MQ=255)
                                                                                               aTTTTGGCAAAGCGTTTTGCGCGGCCCTGAATCTGCACAAAGCCCTGCTCGTCAGAACGCACAATATCGCCAGTGTCATACCAGCCGCGCTCCATTTCGCCGCGAACATTCTCGGCGGTGGGCACTTCCAGTACACCTGGCTTCTCCAcc                                                        >  1:791643/1‑150 (MQ=255)
                                                                                                ttttGGCAAAGCGTTTTGCGCGGCCCTGAATCTGCACAAAGCCCTGCTCGTCAAAACGCACAATATCGCCAGTGTCATACCAGCCGCGCTCCATTTCGCCGCGAACATTCTCGGCGGTGGGCACTTCCAGTACACCTGGCTTCTCCAccc                                                       <  1:4159734/150‑1 (MQ=255)
                                                                                                       aaaGCGTTTTGCGCGGCCCTGAATCTGCACAAAGCCCTGCTCGTCAGAACGCACAATATCGCCAGTGTCATACCAGCCGCGCTCCATTTCGCCGCGAACATTCTCGGCGGTGGGCACTTCCAGTACACCTGGCTTCTCCACCCGCAGATa                                                >  1:3885228/1‑150 (MQ=255)
                                                                                                       aaaGCGTTTTGCGCGGCCCTGAATCTGCACAAAGCCCTGCTCGTCAGAACGCACAATATCGCCAGTGTCATACCAGCCGCGCTCCATTTCGCCGCGAACATTCTCGGCGGTGGGCACTTCCAGTACACCTGGCTTCTCCACCCGCAGATa                                                >  2:4120953/1‑150 (MQ=255)
                                                                                                          gCGTTTTGCGCGGCCCTGAATCTGCACAAAGCCCTGCTCGTCAAAACGCACAATATCGCCAGTGTCATACCAGCCGCGCTCCATTTCGCCGCGAACATTCTCGGCGGTGGGCACTTCCAGTACACCTGGCTTCTCCACCCGCAGATAGcc                                             <  1:3917459/150‑1 (MQ=255)
                                                                                                          gCGTTTTGCGCGGCCCTGAATCTGCACAAAGCCCTGCTCGTCAAAACGCACAATATCGCCAGTGTCATACCAGCCGCGCTCCATTTCGCCGCGAACATTCTCGGCGGTGGGCACTTCCAGTACACCTGGCTTCTCCACCCGCAGATAGcc                                             <  2:4925781/150‑1 (MQ=255)
                                                                                                           agTTTTGCGCGGCCCTGTATCTGCACAATGCCCTGCTCGTCATTACGCACTATCTCGCGTGTGTCATACCAGCCGCGCTCCTTTTCGCCGCGAACATTCTCGGCGGTGGGCACTTCCAGTTCACCTGGCTTCTCCACCCGGTTATAGCCg                                            <  2:1082516/149‑1 (MQ=255)
                                                                                                                 gcgcGGCCCTGAATCTGCACAAAGCCCTGCTCGTCAAAACGCACAATATCGCCAGTGTCATACCAGCCGCGCTCCATTTCGCCGCGAACATTCTCGGCGGTGGGCACTTCCAGTACACCTGGCTTCTCCACCCGCAGATAGCCGTTCAtt                                      <  2:2148394/150‑1 (MQ=255)
                                                                                                                  cgcgGCCCTGAATCTGCACAAAGCCCTGCTCGTCAAAACGCACAATATCGCCAGTGTCATACCAGCCGCGCTCCATTTCGCCGCGAACATTCTCGGCGGTGGGCACTTCCAGTACACCTGGCTTCTCCACCCGCAGATAGCCGTTCATTa                                     >  1:4569147/1‑150 (MQ=255)
                                                                                                                   gcgGCCCTGAATCTGCACAAAGCCCTGCTCGTCAAAACGCACAATATCGCCAGTGTCATACCAGCCGCGCTCCATTTCGCCGCGAACATTCTCGGCGGTGGGCACTTCCAGTACACCTGGCTTCTCCACCCGCAGATAGCCGTTCATTAt                                    >  1:3057539/1‑150 (MQ=255)
                                                                                                                      gCCCTGAATCTGCACAAAGCCCTGCTCGTCAGAACGCACAATATCGCCAGTGTCATACCAGCCGCGCTCCATTTCGCCGCGAACATTCTCGGCGGTGGGCACTTCCAGTACACCTGGCTTCTCCACCCGCAGATAGCCGTTCATTATGtt                                 >  1:3194819/1‑150 (MQ=255)
                                                                                                                      gCCCTGAATCTGCACAAAGCCCTGCTCGTCAAAACGCACAATATCGCCAGTGTCATACCAGCCGCGCTCCATTTCGCCGCGAACATTCTCGGCGGTGGGCACTTCCAGTACACCTGGCTTCTCCACCCGCAGATAGCCGTTCATTATGtt                                 >  1:3439598/1‑150 (MQ=255)
                                                                                                                        ccTGAATCTGCACAAAGCCCTGCTCGTCAAAACGCACAATATCGCCAGTGTCATACCAGCCGCGCTCCATTTCGCCGCGAACATTCTCGGCGGTGGGCACTTCCAGTACACCTGGCTTCTCCACCCGCAGATAGCCGTTCATTATGTTCg                               <  2:4493685/150‑1 (MQ=255)
                                                                                                                         cTGAATCTGCACAAAGCCCTGCTCGTCAAAACGCACAATATCGCCAGTGTCATACCAGCCGCGCTCCATTTCGCCGCGAACATTCTCGGCGGTGGGCACTTCCAGTACACCTGGCTTCTCCACCCGCAGATAGCCGTTCATTATGTTCgg                              <  1:4999663/150‑1 (MQ=255)
                                                                                                                           gAATCTGCACAAAGCCCTGCTCGTCAAAACGCACAATATCGCCAGTGTCATACCAGCCGCGCTCCATTTCGCCGCGAACATTCTCGGCGGTGGGCACTTCCAGTACACCTGGCTTCTCCACCCGCAGATAGCCGTTCATTATGTTCGGcc                            <  1:2525462/150‑1 (MQ=255)
                                                                                                                            aaTCTGCACAAAGCCCTGCTCGTCAGAACGCACAATATCGCCAGTGTCATACCAGCCGCGCTCCATTTCGCCGCGAACATTCTCGGCGGTGGGCACTTCCAGTACACCTGGCTTCTCCACCCGCAGATAGCCGTTCATTATGTTCGGccc                           <  2:1194981/150‑1 (MQ=255)
                                                                                                                             aTCTGCACAAAGCCCTGCTCGTCAGAACGCACAATATCGCCAGTGTCATACCAGCCGCGCTCCATTTCGCCGCGAACATTCTCGGCGGTGGGCACTTCCAGTACACCTGGCTTCTCCACCCGCAGATAGCCGTTCATTATGTTCGGCCCt                          <  2:3474719/150‑1 (MQ=255)
                                                                                                                                     aaaGCCCTGCTCGTCAGAACGCACAATATCGCCAGTGTCATACCAGCCGCGCTCCATTTCGCCGCGAACATTCTCGGCGGTGGGCACTTCCAGTACACCTGGCTTCTCCACCCGCAGATAGCCGTTCATTATGTTCGGCCCTTTCAGTTg                  >  1:788094/1‑150 (MQ=255)
                                                                                                                                     aaaGCCCTGCTCGTCAGAACGCACAATATCGCCAGTGTCATACCAGCCGCGCTCCATTTCGCCGCGAACATTCTCGGCGGTGGGCACTTCCAGTACACCTGGCTTCTCCACCCGCAGATAGCCGTTCATTATGTTCGGCCCTTTCAGTTg                  <  1:3592428/150‑1 (MQ=255)
                                                                                                                                       aGCCCTGCTCGTCAAAACGCACAATATCGCCAGTGTCATACCAGCCGCGCTCCATTTCGCCGCGAACATTCTCGGCGGTGGGCACTTCCAGTACACCTGGCTTCTCCACCCGCAGATAGCCGTTCATTATGTTCGGCCCTTTCAGTTGCa                <  2:2424781/150‑1 (MQ=255)
                                                                                                                                        gCCCTGCTCGTCAAAACGCACAATATCGCCAGTGTCATACCAGCCGCGCTCCATTTCGCCGCGAACATTCTCGGCGGTGGGCACTTCCAGTACACCTGGCTTCTCCACCCGCAGATAGCCGTTCATTATGTTCGGCCCTTTCAGTTGCAg               <  1:1003588/150‑1 (MQ=255)
                                                                                                                                         cccTGCTCGTCAGAACGCACAATATCGCCAGTGTCATACCAGCCGCGCTCCATTTCGCCGCGAACATTCTCGGCGGTGGGCACTTCCAGTACACCTGGCTTCTCCACCCGCAGATAGCCGTTCATTATGTTCGGCCCTTTCAGTTGCAgg              <  1:920070/150‑1 (MQ=255)
                                                                                                                                          ccTGCTCGTCAAAACGCACAATATCGCCAGTGTCATACCAGCCGCGCTCCATTTCGCCGCGAACATTCTCGGCGGTGGGCACTTCCAGTACACCTGGCTTCTCCACCCGCAGATAGCCGTTCATTATGTTCGGCCCTTTCAGTTGCAGGc             >  1:3543627/1‑150 (MQ=255)
                                                                                                                                            tGCTCGTCAAAACGCACAATATCGCCAGTGTCATACCAGCCGCGCTCCATTTCGCCGCGAACATTCTCGGCGGTGGGCACTTCCAGTACACCTGGCTTCTCCACCCGCAGATAGCCGTTCATTATGTTCGGCCCTTTCAGTTGCAGGCGt           <  2:889429/150‑1 (MQ=255)
                                                                                                                                                cGTCAGAACGCACAATATCGCCAGTGTCATACCAGCCGCGCTCCATTTCGCCGCGAACATTCTCGGCGGTGGGCACTTCCAGTACACCTGGCTTCTCCACCCGCAGATAGCCGTTCATTATGTTCGGCCCTTTCAGTTGCAGGCGTCCGc       >  2:525880/1‑150 (MQ=255)
                                                                                                                                                   cAAAACGCACAATATCGCCAGTGTCATACCAGCCGCGCTCCATTTCGCCGCGAACATTCTCGGCGGTGGGCACTTCCAGTACACCTGGCTTCTCCACCCGCAGATAGCCGTTCATTATGTTCGGCCCTTTCAGTTGCAGGCGTCCGCCct    >  1:4479758/1‑150 (MQ=255)
                                                                                                                                                   cAAAACGCACAATATCGCCAGTGTCATACCAGCCGCGCTCCATTTCGCCGCGAACATTCTCGGCGGTGGGCACTTCCAGTACACCTGGCTTCTCCACCCGCAGATAGCCGTTCATTATGTTCGGCCCTTTCAGTTGCAGGCGTCCGCCct    >  1:2613855/1‑150 (MQ=255)
                                                                                                                                                     aaaCGCACAATATCGCCAGTGTCATACCAGCCGCGCTCCATTTCGCCGCGAACATTCTCGGCGGTGGGCACTTCCAGTACACCTGGCTTCTCCACCCGCAGATAGCCGTTCATTATGTTCGGCCCTTTCAGTTGCAGGCGTCCGCCctct  >  2:2607668/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
TGGCATCGCTCTTAATCGCAGTGGCATGGACTTTATCTGGCGAAACACCAAGTGCCAGTTGTTCCACCATTTCCAGCGACACCATTTCGCCTGCAATTTTGGCAAAGCGTTTTGCGCGGCCCTGAATCTGCACAAAGCCCTGCTCGTCAAAACGCACAATATCGCCAGTGTCATACCAGCCGCGCTCCATTTCGCCGCGAACATTCTCGGCGGTGGGCACTTCCAGTACACCTGGCTTCTCCACCCGCAGATAGCCGTTCATTATGTTCGGCCCTTTCAGTTGCAGGCGTCCGCCCTCT  >  CP009273/2967423‑2967721

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑