Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 280,268 G→T 9.4% A453S (GCC→TCC)  yagF → CP4‑6 prophage; dehydratase family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273280,2680GT 9.4% 1335.3 / 73.1 363A453S (GCC→TCC) yagFCP4‑6 prophage; dehydratase family protein
Reads supporting (aligned to +/- strand):  ref base G (156/173);  new base T (13/21);  total (169/194)
Fisher's exact test for biased strand distribution p-value = 3.68e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.91e-01

Only 100 of 374 total aligned reads displayed.

GGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCTCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCACACCGGCCGGGTGCGGGTGTTTGTCTCGGAAGCGCAGGCGATCAAGGCGATCAAGCGGGAAGAGATTGTGCAGGGCGATATCATGGTGGTGATCGGCGGC  >  CP009273/280119‑280417
                                                                                                                                                     |                                                                                                                                                     
gggCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCTCCGGAAGGTTCGGTGATCAAgg                                                                                                                                                       >  1:356132/1‑150 (MQ=255)
gggCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCTCCGGAAGGTTCGGTGATCAAgg                                                                                                                                                       >  1:4816242/1‑150 (MQ=255)
   cgcgcTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCTCCGGAAGGTTCGGTGATCAAGGCCa                                                                                                                                                    >  1:4233970/1‑150 (MQ=255)
   cgcgcTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCTCCGGAAGGTTCGGTGATCAAGGCCa                                                                                                                                                    >  1:4677396/1‑150 (MQ=255)
   cgcgcTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCTCCGGAAGGTTCGGAGATCAAGGCCa                                                                                                                                                    >  1:490853/1‑150 (MQ=255)
           cGCCAGTGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCTCCGGAAGGTTCGGTGATCAAGGCCACGGCGATc                                                                                                                                            <  1:76911/150‑1 (MQ=255)
            gCCAGTGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCTCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCg                                                                                                                                           >  1:659820/1‑150 (MQ=255)
              cAGTGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCTCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGAc                                                                                                                                         <  1:1274122/150‑1 (MQ=255)
               aGTGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCTCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGAcc                                                                                                                                        <  1:2509014/150‑1 (MQ=255)
                 tGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCTCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCg                                                                                                                                      >  2:2988131/1‑150 (MQ=255)
                    cTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCTCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCg                                                                                                                                   <  2:4492411/150‑1 (MQ=255)
                    cTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCTCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCg                                                                                                                                   >  2:4681976/1‑150 (MQ=255)
                    cTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCTCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCg                                                                                                                                   <  2:3906337/150‑1 (MQ=255)
                    cTGCGCGAGCAGGACGGCGGAGAGCCGGATGACGTGCTCCTGCCGCCGGAGAAGGCAAACGCGACCGGGCTGACCTCGCCGGTCTGCTTCCCGACGGGCAACCTCGCTCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCg                                                                                                                                   <  2:1324080/150‑1 (MQ=255)
                      gcgcGAGCAGGACGGCGTAGAGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCTCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCggt                                                                                                                                 <  2:1297826/150‑1 (MQ=255)
                      gcgcGAGCAGGACGGCGTAGAGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCTCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCggt                                                                                                                                 <  2:2022780/150‑1 (MQ=255)
                       cgcgAGCAGGACGGCGTAGAGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCTCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCggtg                                                                                                                                <  2:4802344/150‑1 (MQ=255)
                       cgcgAGCAGGACGGCGTAGAGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCTCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCggtg                                                                                                                                <  2:4928533/150‑1 (MQ=255)
                         cgAGCAGGACGGCGTAGAGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCTCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCggtggt                                                                                                                              <  1:2068996/150‑1 (MQ=255)
                          gAGCAGGACGGCGTAGAGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCTCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGgtggtg                                                                                                                             >  2:2556259/1‑150 (MQ=255)
                          gAGCAGGACGGCGTAGAGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCTCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGgtggtg                                                                                                                             >  1:86899/1‑150 (MQ=255)
                          gAGCAGGACGGCGTAGAGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCTCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGgtggtg                                                                                                                             >  2:5099423/1‑150 (MQ=255)
                           aGCAGGACGGCGTAGAGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCTCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGtggtgg                                                                                                                            <  1:5104735/150‑1 (MQ=255)
                           aGCAGGACGGCGTAGAGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCTCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGtggtgg                                                                                                                            <  2:2698386/150‑1 (MQ=255)
                           aGCAGGACGGCGTAGAGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCTCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGtggtgg                                                                                                                            <  1:2579677/150‑1 (MQ=255)
                            gCAGGACGGCGTAGAGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCTCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTggg                                                                                                                           <  1:4606069/150‑1 (MQ=255)
                              aGGACGGCGTAGAGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCTCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCg                                                                                                                         >  1:2350792/1‑150 (MQ=255)
                               ggACGGCGTAGAGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCTCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGa                                                                                                                        >  1:3835428/1‑150 (MQ=255)
                                   ggCGTAGAGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCTCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGAt                                                                                                                    >  1:813826/1‑150 (MQ=255)
                                   ggCGTAGAGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCTCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGAt                                                                                                                    >  1:1372971/1‑150 (MQ=255)
                                   ggCGTAGAGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCTCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGAt                                                                                                                    >  1:4125151/1‑150 (MQ=255)
                                      gTAGAGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCTCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGc                                                                                                                 >  2:1647638/1‑150 (MQ=255)
                                        agagCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCTCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGt                                                                                                               >  2:2834416/1‑150 (MQ=255)
                                          agCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCTCCGGAAGGTTCGGTGATCAAGTCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGtat                                                                                                             >  1:3993500/1‑150 (MQ=255)
                                          agCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCTCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGtat                                                                                                             <  1:3105242/150‑1 (MQ=255)
                                             cGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCTCCGGAAGGTTCGGTGATCAAGTCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATacc                                                                                                          <  1:2711177/150‑1 (MQ=255)
                                               gATGACGTGATCCTGCCGCCGGAGTCGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCTCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATaccac                                                                                                        >  2:2788363/1‑150 (MQ=255)
                                                 tGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCTCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATAccacca                                                                                                      <  1:5038493/150‑1 (MQ=255)
                                                  gACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCTCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGAGCGAAGATGGCGTATACcaccac                                                                                                     >  2:4011585/1‑150 (MQ=255)
                                                     gTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCTCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCACAcc                                                                                                  <  2:3722603/150‑1 (MQ=255)
                                                             gccgccGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCTCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCACACCGGCcgggt                                                                                          <  1:982592/150‑1 (MQ=255)
                                                                 ccgGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCTCCGGAAGGTTCGGTGATCAAGTCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCACACCGGCcgggtgcgg                                                                                      <  1:1429837/150‑1 (MQ=255)
                                                                    gagaAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCTCCGGAAGGTTCGGTGATCAAGTCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCACACCGGCcgggtgcgggtg                                                                                   >  1:1353023/1‑150 (MQ=255)
                                                                    gagaAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCTCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCACACCGGCcgggtgcgggtg                                                                                   <  1:4011064/150‑1 (MQ=255)
                                                                      gaAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCTCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCACACCGGCCGGGTGCGGGTGtt                                                                                 <  1:4776459/150‑1 (MQ=255)
                                                                       aaGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCTCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCACACCGGCCGGGTGCGGGTGttt                                                                                <  2:4815542/150‑1 (MQ=255)
                                                                         ggCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCTCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCACACCGGCCGGGTGCGGGTGTTTGt                                                                              <  2:3342748/150‑1 (MQ=255)
                                                                         ggCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCTCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCACACCGGCCGGGTGCGGGTGTTTGt                                                                              <  2:3264127/150‑1 (MQ=255)
                                                                          gCAAAAGCTAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCTCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCACCCCGGCCGGGTGCGGGTGTTTGtc                                                                             >  1:2926562/1‑150 (MQ=255)
                                                                          gCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCTCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCACACCGGCCGGGTGCGGGTGTTTGtc                                                                             <  2:3601964/150‑1 (MQ=255)
                                                                           cAACAGCGACAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACAGCGCTCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTAGACCACCACACCGGCCGGGTGCGGGTGTTTGtct                                                                            <  2:1922627/150‑1 (MQ=255)
                                                                              aaGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCTCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCACACCGGCCGGGTGCGGGTGTTTGTCTCgg                                                                         >  2:822768/1‑150 (MQ=255)
                                                                                 cGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCTCCGGAAGGTTCGGTGATCAAGTCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCACACCGGCCGGGTGCGGGTGTTTGTCTCGGAAg                                                                      <  2:1862400/150‑1 (MQ=255)
                                                                                  gAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCTCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCACACCGGCCGGGTGCGGGTGTTTGTCTCGGAAgc                                                                     <  2:780414/150‑1 (MQ=255)
                                                                                  gAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCTCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCACACCGGCCGGGTGCGGGTGTTTGTCTCGGAAgc                                                                     <  2:3585406/150‑1 (MQ=255)
                                                                                   aaaGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCTCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCACACCGGCCGGGTGCGGGTGTTTGTCTCGGAAgcg                                                                    >  2:4258900/1‑150 (MQ=255)
                                                                                    aaGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCTCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCACACCGGCCGGGTGCGGGTGTTTGTCTCGGAAgcgc                                                                   <  2:3479926/150‑1 (MQ=255)
                                                                                    aaGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCTCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCACACCGGCCGGGTGCGGGTGTTTGTCTCGGAAgcgc                                                                   >  1:150395/1‑150 (MQ=255)
                                                                                        gCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCTCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCACACCGGCCGGGTGCGGGTGTTTGTCTCGGAAGCGCaggc                                                               <  2:1005396/150‑1 (MQ=255)
                                                                                         cTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCTCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCACACCGGCCGGGTGCGGGTGTTTGTCTCGGAAGCGCaggcg                                                              <  2:153146/150‑1 (MQ=255)
                                                                                          tGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCTCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCACACCGGCCGGGTGCGGGTGTTTGTCTCGGAAGCGCaggcga                                                             <  1:1524322/150‑1 (MQ=255)
                                                                                            aCCTCGACGGTCTGCTTCCCGACGGGCAACATCGCTCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCACACCGGCCGGGTGCGGGTGTTTGTCTCGGAAGCGCaggcgatc                                                           >  1:299215/1‑150 (MQ=255)
                                                                                            aCCTCGACGGTCTGCTTCCCGACGGGCAACATCGCTCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCACACCGGCCGGGTGCGGGTGTTTGTCTCGGAAGCGCaggcgatc                                                           <  2:1382196/150‑1 (MQ=255)
                                                                                            aCCTCGACGGTCTGCTTCCCGACGGGCAACATCGCTCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCACACCGGCCGGGTGCGGGTGTTTGTCTCGGAAGCGCaggcgatc                                                           <  1:2483556/150‑1 (MQ=255)
                                                                                             ccTCGACGGTCTGCTTCCCGACGGGCAACATCGCTCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCACACCGGCCGGGTGCGGGTGTTTGTCTCGGAAGCGCaggcgatca                                                          <  1:4589087/150‑1 (MQ=255)
                                                                                             ccTCGACGGTCTGCTTCCCGACGGGCAACATCGCTCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCACACCGGCCGGGTGCGGGTGTTTGTCTCGGAAGCGCaggcgatca                                                          >  2:2919807/1‑150 (MQ=255)
                                                                                             ccTCGACGGTCTGCTTCCCGACGGGCAACATCGCTCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCACACCGGCCGGGTGCGGGTGTTTGTCTCGGAAGCGCaggcgatca                                                          >  2:180708/1‑150 (MQ=255)
                                                                                                   cGGTCTGCTTCCCGACGGGCAACATCGCTCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCACACCGGCCGGGTGCGGGTGTTTGTCTCGGAAGCGCaggcgatcaaggcga                                                    <  1:1720106/150‑1 (MQ=255)
                                                                                                    ggTCTGCTTCCCGACGGGCAACATCGCTCCGGAAGGTTCGGTGATCAAGTCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCACACCGGCCGGGTGCGGGTGTTTGTCTCGGAAGCGCaggcgatcaaggcgat                                                   >  2:464112/1‑150 (MQ=255)
                                                                                                    ggTCTGCTTCCCGACGGGCAACATCGCTCCGGAAGGTTCGGTGATCAAGTCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCACACCGGCCGGGTGCGGGTGTTTGTCTCGGAAGCGCaggcgatcaaggcgat                                                   >  2:5035279/1‑150 (MQ=255)
                                                                                                    ggTCTGCTTCCCGACGGGCAACATCGCTCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCACACCGGCCGGGTGCGGGTGTTTGTCTCGGAAGCGCaggcgatcaaggcgat                                                   >  1:4709397/1‑150 (MQ=255)
                                                                                                     gTCTGCTTCCCGACGGGCAACATCGCTCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCACACCGGCCGGGTGCGGGTGTTTGTCTCGGAAGCGCaggcgatcaaggcgatc                                                  <  1:3261539/150‑1 (MQ=255)
                                                                                                     gTCTGCTTCCCGACGGGCAACATCGCTCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCACACCGGCCGGGTGCGGGTGTTTGTCTCGGAAGCGCaggcgatcaaggcgatc                                                  <  1:26978/150‑1 (MQ=255)
                                                                                                       cTGCTTCCCGACGGGCAACATCGCTCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCACACCGGCCGGGTGCGGGTGTTTGTCTCGGAAGCGCAggcgatcaaggcgatcaa                                                <  1:2235716/150‑1 (MQ=255)
                                                                                                         gCTTCCCGACGGGCAACATCGCTCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCACACCGGCCGGGTGCGGGTGTTTGTCTCGGAAGCGCAGGCGATCAAGGCGATCAAGc                                              <  1:1095657/150‑1 (MQ=255)
                                                                                                         gCTTCCCGACGGGCAACATCGCTCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCACACCGGCCGGGTGCGGGTGTTTGTCTCGGAAGCGCAGGCGATCAAGGCGATCAAGc                                              >  1:2891057/1‑150 (MQ=255)
                                                                                                            tCCCGACGGGCAACATCGCTCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCACACCGGCCGGGTGCGGGTGTTTGTCTCGGAAGCGCAGGCGATCAAGGCGATCAAGCggg                                           >  2:5142493/1‑150 (MQ=255)
                                                                                                                  cGGGCAACATCGCTCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCACACCGGCCGGGTGCGGGTGTTTGTCTCGGAAGCGCAGGCGATCAAGGCGATCAAGCGGGAAgaga                                     >  2:4996716/1‑150 (MQ=255)
                                                                                                                      cAACATCGCTCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCACACCGGCCGGGTGCGGGTGTTTGTCTCGGAAGCGCAGGCGATCAAGGCGATCAAGCGGGAAGAGATtgt                                 <  1:2163374/150‑1 (MQ=255)
                                                                                                                      cAACATCGCTCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCACACCGGCCGGGTGCGGGTGTTTGTCTCGGAAGCGCAGGCGATCAAGGCGATCAAGCGGGAAGAGATtgt                                 <  1:3513737/150‑1 (MQ=255)
                                                                                                                       aaCATCGCTCCGGAAGGTTCGGTGATCAAGTCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCACACCGGCCGGGTGCGGGTGTTTGTCTCGGAAGCGCAGGCGATCAAGGCGATCAAGCGGGAAGAGATtgtg                                <  1:3161187/150‑1 (MQ=255)
                                                                                                                       aaCATCGCTCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCACACCGGCCGGGTGCGGGTGTTTGTCTCGGAAGCGCAGGCGATCAAGGCGATCAAGCGGGAAGAGATtgtg                                <  2:1054713/150‑1 (MQ=255)
                                                                                                                         cATCGCTCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCACACCGGCCGGGTGCGGGTGTTTGTCTCGGAAGCGCAGGCGATCAAGGCGATCAAGCGGGAAGAGATTGTGCa                              <  1:3045799/150‑1 (MQ=255)
                                                                                                                            cGCTCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCACACCGGCCGGGTGCGGGTGTTTGTCTCGGAAGCGCAGGCGATCAAGGCGATCAAGCGGGAAGAGATTGTGCAggg                           >  1:4335650/1‑150 (MQ=255)
                                                                                                                             gCTCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCACACCGGCCGGGTGCGGGTGTTTGTCTCGGAAGCGCAGGCGATCAAGGCGATCAAGCGGGAAGAGATTGTGCAGGGc                          <  2:2439612/150‑1 (MQ=255)
                                                                                                                                 cGGAAGGTTCGGTGATCAAGTCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCACACCGGCCGGGTGCGGGTGTTTGTCTCGGAAGCGCAGGCGATCAAGGCGATCAAGCGGGAAGAGATTGTGCAGGGCGata                      >  1:3409235/1‑150 (MQ=255)
                                                                                                                                  ggAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCACACCGGCCGGGTGCGGGTGTTTGTCTCGGAAGCGCAGGCGATCAAGGCGATCAAGCGGGAAGAGATTGTGCAGGGCGatat                     >  1:141283/1‑150 (MQ=255)
                                                                                                                                       gTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCACACCGGCCGGGTGCGGGTGTTTGTCTCGGAAGCGCAGGCGATCAAGGCGATCAAGCGGGAAGAGATTGTGCAGGGCGATATCAtgg                <  1:3092788/150‑1 (MQ=255)
                                                                                                                                        ttCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCACACCGGCCGGGTGCGGGTGTTTGTCTCGGAAGCGCAGGCGATCAAGGCGATCAAGCGGGAAGAGATTGTGCAGGGCGATATCAtggt               <  1:2836837/150‑1 (MQ=255)
                                                                                                                                         tCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCACACCGGCCGGGTGCGGGTGTTTGTCTCGGAAGCGCAGGCGATCAAGGCGATCAAGCGGGAAGAGATTGTGCAGGGCGATATCAtggtg              >  2:2612895/1‑150 (MQ=255)
                                                                                                                                           ggTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCACACCGGCCGGGTGCGGGTGTTTGTCTCGGAAGCGCAGGCGATCAAGGCGATCAAGCGGGAAGAGATTGTGCAGGGCGATATCATggtggt            >  2:570783/1‑150 (MQ=255)
                                                                                                                                             tGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCACACCGGCCGGGTGCGGGTGTTTGTCTCGGAAGCGCAGGCGATCAAGGCGATCAAGCGGGAAGAGATTGTGCAGGGCGATATCATGGTGGTGa          >  2:1958914/1‑150 (MQ=255)
                                                                                                                                               aTCTAGGCCACGGCGATCGTCCCGTGGGTGGGGGGCGAAGATGGCGTTTTGCTCCACTCCGGGCGGGTGCGGGTGTTTGTCTCGGACGCGCAGGCGATCACGGCGATCAAGCGGGAAGAGATTGTGCAGGGAGATATCTTGGTGGTGATc        <  2:2135297/150‑1 (MQ=255)
                                                                                                                                               aTCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCACACCGGCCGGGTGCGGGTGTTTGTCTCGGAAGCGCAGGCGATCAAGGCGATCAAGCGGGAAGAGATTGTGCAGGGCGATATCATGGTGGTGATc        <  1:3193704/150‑1 (MQ=255)
                                                                                                                                               aTCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCACACCGGCCGGGTGCGGGTGTTTGTCTCGGAAGCGCAGGCGATCAAGGCGATCAAGCGGGAAGAGATTGTGCAGGGCGATATCATGGTGGTGATc        <  1:180708/150‑1 (MQ=255)
                                                                                                                                                tCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCACACCGGCCGGGTGCGGGTGTTTGTCTCGGAAGCGCAGGCGATCAAGGCGATCAAGCGGGAAGAGATTGTGCAGGGCGATATCATGGTGGTGATcg       <  2:1400482/150‑1 (MQ=255)
                                                                                                                                                 cAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCACACCGGCCGGGTGCGGGTGTTTGTCTCGGAAGCGCAGGCGATCAAGGCGATCAAGCGGGAAGAGATTGTGCAGGGCGATATCATGGTGGTGATcgg      <  1:2024119/150‑1 (MQ=255)
                                                                                                                                                  aaGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCACACCGGCCGGGTGCGGGTGTTTGTCTCGGAAGCGCAGGCGATCAAGGCGATCAAGCGGGAAGAGATTGTGCAGGGCGATATCATGGTGGTGATcggc     >  2:219128/1‑150 (MQ=255)
                                                                                                                                                   aGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCACACCGGCCGGGTGCGGGTGTTTGTCTCGGAAGCGCAGGCGATCAAGGCGATCAAGCGGGAAGAGATTGTGCAGGGCGATATCATGGTGGTGATcggcg    >  1:1020995/1‑150 (MQ=255)
                                                                                                                                                    ggCCTCGGCGATCGACCCGTCGGTGGTGGGCGTAGATGGCGTATACCACCCCACCGGCCGGGTGCGGGTGTTTGTCTCGGAAGCGCAGGCGATCATGGCGATCAAGCGGGAAGATATTGTGCAGGGCGATATCATGGTTGTGATcggcgt   <  2:630254/150‑2 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
GGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCTCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCACACCGGCCGGGTGCGGGTGTTTGTCTCGGAAGCGCAGGCGATCAAGGCGATCAAGCGGGAAGAGATTGTGCAGGGCGATATCATGGTGGTGATCGGCGGC  >  CP009273/280119‑280417

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑