Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,064,914 G→T 82.0% I356I (ATC→ATA)  pgk ← phosphoglycerate kinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,064,9140GT82.0% 719.8 / 203.1 349I356I (ATC→ATA) pgkphosphoglycerate kinase
Reads supporting (aligned to +/- strand):  ref base G (28/35);  new base T (133/153);  total (161/188)
Fisher's exact test for biased strand distribution p-value = 7.82e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.71e-01

Only 100 of 355 total aligned reads displayed.

GTGCTCTAAAAGCGCGCTGAAACAAGGGCAGGTTTCCCTGCCCTGTGATTTTTTACTTCTTAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGAGATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAGCCACGATTTCAGTACCTTTGCGGAAGTTCGGGAATTCGAACACGCCAACCGGACC  >  CP009273/3064766‑3065060
                                                                                                                                                    |                                                                                                                                                  
    tctAAAAGCGCGCTGAAACAAGGGCAGGTTTCCCTGCCCTGTGATTTTTTACTTCTTAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATAtttt                                                                                                                                               >  1:1668750/1‑150 (MQ=255)
    tctAAAAGCGCGCTGAAACAAGGGCAGGTTTCCCTGCCCTGTGATTTTTTACTTCTTAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGAGAtttt                                                                                                                                               <  1:310722/150‑1 (MQ=255)
           gcgcgcTGAAACAAGGGCAGGTTTCCCTGCCCTGTGATTTTTTACTTCTTAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCa                                                                                                                                        <  1:4599921/150‑1 (MQ=255)
            cgcgcTGAAACAAGGGCAGGTTTCCCTGCCCTGTGATTTTTTACTTCTTGGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGAGATTTTGTCAGCaa                                                                                                                                       <  2:3482897/150‑1 (MQ=255)
               gcTGAAACAAGGGCAGGTTTCCCTGCCCTGTGATTTTTTACTTCTTAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGc                                                                                                                                    <  2:883998/150‑1 (MQ=255)
               gcTGAAACAAGGGCAGGTTTCCCTGCCCTGTGATTTTTTACTTCTTAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGc                                                                                                                                    <  1:4487820/150‑1 (MQ=255)
                cTGAAACAAGGGCAGGTTTCCCTGCCCTGTGATTTTTTACTTCTTAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGcc                                                                                                                                   <  1:1575792/150‑1 (MQ=255)
                cTGAAACAAGGGCAGGTTTCCCTGCCCTGTGATTTTTTACTTCTTAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGAGATTTTGTCAGCAATGcc                                                                                                                                   <  2:220388/150‑1 (MQ=255)
                 tGAAACAAGGGCAGGTTTCCCTGCCCTGTGATTTTTTACTTCTTAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCg                                                                                                                                  >  1:2861330/1‑150 (MQ=255)
                  gAAACAAGGGCAGGTTTCCCTGCCCTGTGATTTTTTACTTCTTAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGa                                                                                                                                 <  1:2929457/150‑1 (MQ=255)
                   aaaCAAGGGCAGGTTTCCCTGCCCTGTGATTTTTTACTTCTTAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGaa                                                                                                                                <  1:2798202/150‑1 (MQ=255)
                       aaGGGCAGGTTTCCCTGCCCTGTGATTTTTTACTTCTTAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAgg                                                                                                                            >  2:2572889/1‑150 (MQ=255)
                       aaGGGCAGGTTTCCCTGCCCTGTGATTTTTTACTTCTTAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGAGATTTTTTCAGCAATGCCGAACAgg                                                                                                                            >  1:2738502/1‑150 (MQ=255)
                       aaGGGCAGGTTTCCCTGCCCTGTGATTTTTTACTTCTTAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGAGATTTTGTCAGCAATGCCGAACAgg                                                                                                                            >  1:861052/1‑150 (MQ=255)
                         gggCAGGTTTCCCTGCCCTGTGATTTTTTACTTCTTAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCACTGCCGAACAGGTc                                                                                                                          >  1:4948976/1‑150 (MQ=255)
                               gTTTCCCTGCCCTGTGATTTTTTACTTCTTAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATtgc                                                                                                                    >  2:2394707/1‑150 (MQ=255)
                               gTTTCCCTGCCCTGTGATTTTTTACTTCTTAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATtgc                                                                                                                    <  2:2363794/150‑1 (MQ=255)
                                  tCCCTGCCCTGTGATTTTTTACTTCTTAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATtgctgc                                                                                                                 <  2:3720464/150‑1 (MQ=255)
                                   ccctgccctgTGATTTTTTACTTCTTAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGcc                                                                                                                <  1:1001081/150‑1 (MQ=255)
                                     ctgccctgTGATTTTTTACTTCTTAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCag                                                                                                              <  1:230804/150‑1 (MQ=255)
                                      tgccctgTGATTTTTTACTTCTTAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCaga                                                                                                             >  2:4710840/1‑150 (MQ=255)
                                        ccctgTGATTTTTTACTTCTTAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAgt                                                                                                           <  1:317323/150‑1 (MQ=255)
                                           tgtgATTTTTTACTTCTTAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTc                                                                                                        <  1:4174993/150‑1 (MQ=255)
                                            gtgATTTTTTACTTCTTAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTcg                                                                                                       <  1:192527/150‑1 (MQ=255)
                                            gtgATTTTTTACTTCTTAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGAGATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTcg                                                                                                       >  1:4027600/1‑150 (MQ=255)
                                             tgATTTTTTACTTCTTAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTcgc                                                                                                      <  2:2384600/150‑1 (MQ=255)
                                               aTTTTTTACTTCTTAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTcgccg                                                                                                    <  2:5122146/150‑1 (MQ=255)
                                                 tttttACTTCTTAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTcgccgcc                                                                                                  <  1:3636765/150‑1 (MQ=255)
                                                 tttttACTTCTTAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTcgccgcc                                                                                                  >  2:5071668/1‑150 (MQ=255)
                                                 tttttACTTCTTAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTcgccgcc                                                                                                  >  1:4760644/1‑150 (MQ=255)
                                                 tttttACTTCTTAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATACGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTcgccgcc                                                                                                  <  2:1058043/150‑1 (MQ=255)
                                                 tttttACTTCTTAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTGACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGAGATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTcgccgcc                                                                                                  <  1:4895179/150‑1 (MQ=255)
                                                  ttttACTTCTTAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTcgccgccg                                                                                                 >  2:1484898/1‑150 (MQ=255)
                                                  ttttACTTCTTAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTcgccgccg                                                                                                 >  2:1928313/1‑150 (MQ=255)
                                                        ttcttAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCg                                                                                           <  2:4432776/150‑1 (MQ=255)
                                                        ttcttAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCg                                                                                           >  2:3965550/1‑150 (MQ=255)
                                                          cttAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGAt                                                                                         <  1:1463757/150‑1 (MQ=255)
                                                          cttAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGAt                                                                                         >  2:2610951/1‑150 (MQ=255)
                                                            tAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATgg                                                                                       >  2:1987603/1‑150 (MQ=255)
                                                            tAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATgg                                                                                       >  1:4247520/1‑150 (MQ=255)
                                                               cgcgcTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGgaga                                                                                    <  1:985945/150‑1 (MQ=255)
                                                                 cgcTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGAGATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAAc                                                                                  >  2:2094017/1‑150 (MQ=255)
                                                                   ctctTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGAGATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGc                                                                                >  2:691040/1‑150 (MQ=255)
                                                                    tctTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCt                                                                               >  2:4461676/1‑150 (MQ=255)
                                                                      ttCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTc                                                                             >  1:5129933/1‑150 (MQ=255)
                                                                      ttCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGAGATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTc                                                                             <  2:2039120/150‑1 (MQ=255)
                                                                        cGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCTCCGCCGCCAGCGATGGAGAACGCTTCGc                                                                           >  1:1097200/1‑150 (MQ=255)
                                                                              tCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTg                                                                     <  1:3562475/150‑1 (MQ=255)
                                                                                gCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCg                                                                   <  1:3837105/150‑1 (MQ=255)
                                                                                 cTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGa                                                                  >  1:1415280/1‑150 (MQ=255)
                                                                                  tACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGAt                                                                 >  2:305500/1‑150 (MQ=255)
                                                                                  tACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGAt                                                                 >  2:2900989/1‑150 (MQ=255)
                                                                                   aCTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATa                                                                <  2:4644385/150‑1 (MQ=255)
                                                                                   aCTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATa                                                                <  1:3442170/150‑1 (MQ=255)
                                                                                   aCTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGAGATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATa                                                                >  1:692730/1‑150 (MQ=255)
                                                                                    cTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAg                                                               >  2:4372657/1‑150 (MQ=255)
                                                                                         ggcagTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGAGATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTa                                                          <  2:2531423/150‑1 (MQ=255)
                                                                                          gcagTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGAGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAg                                                         >  2:941470/1‑150 (MQ=255)
                                                                                                cTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAGCCACGa                                                   <  1:4710840/150‑1 (MQ=255)
                                                                                                cTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGAGATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAGCCACGa                                                   >  1:3070522/1‑150 (MQ=255)
                                                                                                 tttACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAGCCACGAt                                                  <  2:1638618/150‑1 (MQ=255)
                                                                                                  ttACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAGCCACGAtt                                                 >  2:4925073/1‑150 (MQ=255)
                                                                                                    aCCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAGCCACGATTTc                                               >  1:4810783/1‑150 (MQ=255)
                                                                                                      cTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAGCCACGATTTCAg                                             >  2:3122808/1‑150 (MQ=255)
                                                                                                       ttCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAGCCACGATTTCAGt                                            <  1:197251/150‑1 (MQ=255)
                                                                                                       ttCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAGCCACGATTTCAGt                                            >  1:798625/1‑150 (MQ=255)
                                                                                                         ccACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATATTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAGCCACGATTTCAGTAc                                          <  2:338847/150‑1 (MQ=255)
                                                                                                          cACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAGCCACGATTTCAGTAcc                                         >  1:140783/1‑150 (MQ=255)
                                                                                                           aCGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAGCCACGATTTCAGTACCt                                        <  1:598853/150‑1 (MQ=255)
                                                                                                            cGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAGCCACGATTTCAGTACCtt                                       <  2:4618917/150‑1 (MQ=255)
                                                                                                             gAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAGCCACGATTTCAGTACCttt                                      <  2:1702840/150‑1 (MQ=255)
                                                                                                              aaTTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAGCCACGATTTCAGTACCTTTg                                     <  1:3965550/150‑1 (MQ=255)
                                                                                                              aaTTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAGCCACGATTTCAGTACCTTTg                                     <  2:4213235/150‑1 (MQ=255)
                                                                                                                   gAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAGCCACGATTTCAGTACCTTTGCGGaa                                >  2:4522922/1‑150 (MQ=255)
                                                                                                                   gAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAGCCACGATTTCAGTACCTTTGCGGaa                                >  2:3379535/1‑150 (MQ=255)
                                                                                                                   gAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAGCCACGATTTCAGTACCTTTGCGGaa                                >  2:4737273/1‑150 (MQ=255)
                                                                                                                    aGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAGCCACGATTTCAGTACCTTTGCGGAAg                               >  1:732629/1‑150 (MQ=255)
                                                                                                                    aGGAATGCGCCGCCGCCAGTGGAGATGTAGGAGATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAGCCACGATTTCAGTACCTTTGCGGAAg                               >  2:4837172/1‑150 (MQ=255)
                                                                                                                     ggAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAGCCACGATTTCAGTACCTTTGCGGAAGt                              <  1:2075761/150‑1 (MQ=255)
                                                                                                                     ggAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAGCCACGATTTCAGTACCTTTGCGGAAGt                              <  1:3790931/150‑1 (MQ=255)
                                                                                                                     ggAATGCGCCGCCGCCAGTGGAGATGTAGGAGATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAGCCACGATTTCAGTACCTTTGCGGAAGt                              <  1:359619/150‑1 (MQ=255)
                                                                                                                      gAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAGCCACGATTTCAGTACCTTTGCGGAAGtt                             <  2:4149304/150‑1 (MQ=255)
                                                                                                                       aaTGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAGCCACGATTTCAGTACCTTTGCGGAAGTTc                            <  2:1273723/150‑1 (MQ=255)
                                                                                                                       aaTGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAGCCACGATTTCAGTACCTTTGCGGAAGTTc                            <  2:2241567/150‑1 (MQ=255)
                                                                                                                        aTGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAGCCACGATTTCAGTACCTTTGCGGAAGTTCg                           <  1:2684363/150‑1 (MQ=255)
                                                                                                                            gccgccgccAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAGCCACGATTTCAGTACCTTTGCGGAAGTTCGGGaa                       >  1:440888/1‑150 (MQ=255)
                                                                                                                              cgccgccAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAGCCACGATTTCAGTACCTTTGCGGAAGTTCGGGAAtt                     <  1:925585/150‑1 (MQ=255)
                                                                                                                              cgccgccAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAGCCACGATTTCAGTACCTTTGCGGAAGTTCGGGAAtt                     >  1:1477546/1‑150 (MQ=255)
                                                                                                                               gccgccAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAGCCACGATTTCAGTACCTTTGCGGAAGTTCGGGAATTc                    >  2:3016236/1‑150 (MQ=255)
                                                                                                                                  gccAGTGGAGATGTAGGAGATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAGCCACGATTTCAGTACCTTTGCGGAAGTTCGGGAATTCGaa                 >  1:4220615/1‑150 (MQ=255)
                                                                                                                                  gccAGGGGAGATGGAGGATATTTTGTCAGCAATGCCGACCAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAGCCACGAGTTCAGTACCATTGCGGAAGTTCGGGAAGTCGaa                 >  2:4566610/1‑150 (MQ=255)
                                                                                                                                     aGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAGCCACGATTTCAGTACCTTTGCGGAAGTTCGGGAATTCGAacac              >  1:5121481/1‑150 (MQ=255)
                                                                                                                                      gTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAGCCACGATTTCAGTACCTTTGCGGAAGTTCGGGAATTCGAACACg             <  1:2355900/150‑1 (MQ=255)
                                                                                                                                         gagaTGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAGCCACGATTTCAGTACCTTTGCGGAAGTTCGGGAATTCGAACACGCCa          <  1:2900989/150‑1 (MQ=255)
                                                                                                                                         gagaTGTAGGAGATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAGCCACGATTTCAGTACCTTTGCGGAAGTTCGGGAATTCGAACACGCCa          <  1:1779436/150‑1 (MQ=255)
                                                                                                                                         gagaTGTAGGAGATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAGCCACGATTTCAGTACCTTTGCGGAAGTTCGGGAATTCGAACACGCCa          >  2:1104145/1‑150 (MQ=255)
                                                                                                                                         gagaTGTAGGAGATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAGCCACGATTTCAGTACCTTTGCGGAAGTTCGGGAATTCGAACACGCCa          >  2:1782984/1‑150 (MQ=255)
                                                                                                                                          agaTGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAGCCACGATTTCAGTACCTTTGCGGAAGTTCGGGAATTCGAACACGCCaa         >  2:2480024/1‑150 (MQ=255)
                                                                                                                                           gaTGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAGCCACGATTTCAGTACCTTTGCGGAAGTTCGGGAATTCGAACACGCCAAc        >  1:185686/1‑150 (MQ=255)
                                                                                                                                               tAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAGCCACGATTTCAGTACCTTTGCGGAAGTTCGGGAATTCGAACACGCCAACCGGa    >  1:4396775/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
GTGCTCTAAAAGCGCGCTGAAACAAGGGCAGGTTTCCCTGCCCTGTGATTTTTTACTTCTTAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGAGATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAGCCACGATTTCAGTACCTTTGCGGAAGTTCGGGAATTCGAACACGCCAACCGGACC  >  CP009273/3064766‑3065060

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑