Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,076,526 C→A 100% D211Y (GAC→TAC)  speB ← agmatinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,076,5260CA95.6% 1012.8 / 30.9 304D211Y (GAC→TAC) speBagmatinase
Reads supporting (aligned to +/- strand):  ref base C (5/9);  new base A (146/144);  total (151/153)
Fisher's exact test for biased strand distribution p-value = 4.13e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 304 total aligned reads displayed.

GTACCAGTTTAATAGCGCGATCGGAGGTCAGGCCGCCAATCACTGGCGTACCGGTGCCTGGTGCAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTCATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGAATGATTCGGGTCGATCAGACCTTCTTTCGGCGCGGTATAGAACATAGTGCCGTGG  >  CP009273/3076379‑3076671
                                                                                                                                                   |                                                                                                                                                 
gTACCAGTTTAATAGCGCGATCGGAGGTCAGGCCGCCAATCACTGGCGTACCGGTGCCTGGTGCAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAAt                                                                                                                                                 <  1:3187291/150‑1 (MQ=255)
    cAGTTTAATAGCGCGATCGGAGGTCAGGCCGCCAATCACTGGCGTACCGGTGCCTGGTGCAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCAc                                                                                                                                             >  1:4554506/1‑150 (MQ=255)
     aGTTTAATAGCGCGATCGGAGGTCAGGCCGCCAATCACTGGCGTACCGGTGCCTGGTGCAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTCATCCACg                                                                                                                                            <  2:2633501/150‑1 (MQ=255)
        ttAATAGCGCGATCGGAGGTCAGGCCGCCAATCACTGGCGTACCGGTGCCTGGTGCAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTg                                                                                                                                         >  2:4807426/1‑150 (MQ=255)
         tAATAGCGCGATCGGAGGTCAGGCCGCCAATCACTGGCGTACCGGTGCCTGGTGCAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGc                                                                                                                                        <  2:2860153/150‑1 (MQ=255)
          aaTAGCGCGATCGGAGGTCAGGCCGCCAATCACTGGCGTACCGGTGCCTGGTGCAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCg                                                                                                                                       <  2:4041335/150‑1 (MQ=255)
           aTAGCGCGATCGGAGGTCAGGCCGCCAATCACTGGCGTACCGGTGCCTGGTGCAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGa                                                                                                                                      >  2:3830242/1‑150 (MQ=255)
            tAGCGCGATCGGAGGTCAGGCCGCCAATCACTGGCGTACCGGTGCCTGGTGCAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGAt                                                                                                                                     <  2:314601/150‑1 (MQ=255)
                  gATCGGAGGTCAGGCCGCCAATCACTGGCGTACCGGTGCCTGGTGCAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCa                                                                                                                               >  2:1187506/1‑150 (MQ=255)
                  gATCGGAGGTCAGGCCGCCAATCACTGGCGTACCGGTGCCTGGTGCAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCa                                                                                                                               >  1:1850333/1‑150 (MQ=255)
                     cGGAGGTCAGGCCGCCAATCACTGGCGTACCGGTGCCTGGTGCAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCt                                                                                                                            <  1:2975692/150‑1 (MQ=255)
                     cGGAGGTCAGGCCGCCAATCACTGGCGTACCGGTGCCTGGTGCAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCt                                                                                                                            <  1:2911356/150‑1 (MQ=255)
                      ggAGGTCAGGCCGCCAATCACTGGCGTACCGGTGCCTGGTGCAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCta                                                                                                                           <  2:3334812/150‑2 (MQ=255)
                      ggAGGTCAGGCCGCCAATCACTGGCGTACCGGTGCCTGGTGCAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTg                                                                                                                           >  2:4548458/1‑150 (MQ=255)
                      ggAGGTCAGGCCGCCAATCACTGGCGTACCGGTGCCTGGTGCAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTg                                                                                                                           >  1:3759664/1‑150 (MQ=255)
                      ggAGGTCAGGCCGCCAATCACTGGCGTACCGGTGCCTGGTGCAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTg                                                                                                                           >  2:4017342/1‑150 (MQ=255)
                      ggAGGTCAGGCCGCCAATCACTGGCGTACCGGTGCCTGGTGCAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTg                                                                                                                           >  1:5066529/1‑150 (MQ=255)
                       gAGGTCAGGCCGCCAATCACTGGCGTACCGGTGCCTGGTGCAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTCATCCACGCTGCGATCGTTCACCTgg                                                                                                                          >  2:435398/1‑150 (MQ=255)
                         ggTCAGGCCGCCAATCACTGGCGTACCGGTGCCTGGTGCAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCa                                                                                                                        <  1:1342565/150‑1 (MQ=255)
                           tCAGGCCGCCAATCACTGGCGTACCGGTGCCTGGTGCAAAAGCAGGATCCAGGCAGTCGATATCACAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAgg                                                                                                                      <  2:3516628/150‑1 (MQ=255)
                           tCAGGCCGCCAATCACTGGCGTACCGGTGCCTGGTGCAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAgg                                                                                                                      >  1:2586574/1‑150 (MQ=255)
                           tCAGGCCGCCAATCACTGGCGTACCGGTGCCTGGTGCAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAgg                                                                                                                      <  1:969075/150‑1 (MQ=255)
                             aGGCCGCCAATCACTGGCGTACCGGTGCCTGGTGCAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCg                                                                                                                    >  1:2455347/1‑150 (MQ=255)
                             aGGCCGCCAATCACTGGCGTACCGGTGCCTGGTGCAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCg                                                                                                                    >  1:639031/1‑150 (MQ=255)
                              ggCCGCCAATCACTGGCGTACCGGTGCCTGGTGCAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGt                                                                                                                   <  1:3189869/150‑1 (MQ=255)
                                  gccAATCACTGGCGTACCGGTGCCTGGTGCAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAg                                                                                                               <  1:2161674/150‑1 (MQ=255)
                                    cAATCACTGGCGTACCGGTGCCTGGTGCAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCa                                                                                                             >  2:2755034/1‑150 (MQ=255)
                                     aaTCACTGGCGTACCGGTGCCTGGTGCAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTcc                                                                                                                 <  1:2845789/145‑1 (MQ=255)
                                     aaTCACTGGCGTACCGGTGCCTGGTGCAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCAc                                                                                                            <  2:3180214/150‑1 (MQ=255)
                                     aaTCACTGGCGTACCGGTGCCTGGTGCAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCAc                                                                                                            >  1:1020242/1‑150 (MQ=255)
                                         aCTGGCGTACCGGTGCCTGGTGCAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTa                                                                                                        <  1:4928167/150‑1 (MQ=255)
                                         aCTGGCGTACCGGTGCCTGGTGCAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTa                                                                                                        <  1:485772/150‑1 (MQ=255)
                                           tGGCGTACCGGTGCCTGGTGCAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTTGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTaaa                                                                                                      <  1:3061604/150‑1 (MQ=255)
                                           tGGCGTACCGGTGCCTGGTGCAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTaaa                                                                                                      >  2:2565978/1‑150 (MQ=255)
                                            ggCGTACCGGTGCCTGGTGCAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAg                                                                                                     >  2:611282/1‑150 (MQ=255)
                                              cGTACCGGTGCCTGGTGCAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGcc                                                                                                   <  2:5130839/150‑1 (MQ=255)
                                               gTACCGGTGCCTGGTGCAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCg                                                                                                  >  1:4915374/1‑150 (MQ=255)
                                               gTACCGGTGCCTGGTGCAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCg                                                                                                  >  2:1542932/1‑150 (MQ=255)
                                                 aCCGGTGCCTGGTGCAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGtt                                                                                                >  1:5036569/1‑150 (MQ=255)
                                                   cGGTGCCTGGTGCAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGt                                                                                              <  2:1739433/150‑1 (MQ=255)
                                                     gTGCCTGGTGCAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCt                                                                                            <  2:737065/150‑1 (MQ=255)
                                                       gCCTGGTGCAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCttt                                                                                          <  1:1613341/150‑1 (MQ=255)
                                                         cTGGTGCAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTAt                                                                                        >  2:238172/1‑150 (MQ=255)
                                                         cTGGTGCAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTAt                                                                                        <  1:107698/150‑1 (MQ=255)
                                                           ggTGCAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCa                                                                                      >  2:2882131/1‑150 (MQ=255)
                                                            gTGCAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCaa                                                                                     >  1:4811170/1‑150 (MQ=255)
                                                             tGCAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCaaa                                                                                    <  1:1168567/150‑1 (MQ=255)
                                                               cAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACt                                                                                  >  1:3862740/1‑150 (MQ=255)
                                                               cAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACt                                                                                  <  1:2530759/150‑1 (MQ=255)
                                                                  aaGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCgg                                                                               >  1:4761299/1‑150 (MQ=255)
                                                                     cAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTAc                                                                            <  1:395608/150‑1 (MQ=255)
                                                                      aGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACg                                                                           >  1:199063/1‑150 (MQ=255)
                                                                         aTCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAAt                                                                        <  1:176608/150‑1 (MQ=255)
                                                                           ccAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATAc                                                                      <  1:406020/150‑1 (MQ=255)
                                                                            cAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATAcc                                                                     >  1:4617440/1‑150 (MQ=255)
                                                                            cAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATAcc                                                                     >  2:2609003/1‑150 (MQ=255)
                                                                             aGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCa                                                                    >  2:3732585/1‑150 (MQ=255)
                                                                                 aGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCt                                                                >  2:1556427/1‑150 (MQ=255)
                                                                                  gTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTg                                                               >  1:3329256/1‑150 (MQ=255)
                                                                                    cGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCa                                                             <  2:4173103/150‑1 (MQ=255)
                                                                                     gATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCAc                                                            <  2:837341/150‑1 (MQ=255)
                                                                                      atatCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACg                                                           >  2:1156625/1‑150 (MQ=255)
                                                                                      atatCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACg                                                           >  2:1074091/1‑150 (MQ=255)
                                                                                       tatCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACgg                                                          >  2:2678503/1‑150 (MQ=255)
                                                                                        atCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGa                                                         <  2:1966818/150‑1 (MQ=255)
                                                                                         tCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGaa                                                        >  1:4203596/1‑150 (MQ=255)
                                                                                          cAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGAAt                                                       <  2:3666554/150‑1 (MQ=255)
                                                                                           aaaaGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGAATg                                                      >  1:3432693/1‑150 (MQ=255)
                                                                                              aGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGAATGAtt                                                   >  2:569421/1‑150 (MQ=255)
                                                                                               gTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGAATGATTc                                                  >  2:2918975/1‑150 (MQ=255)
                                                                                                  aGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGAATGATTCggg                                               >  2:3565382/1‑150 (MQ=255)
                                                                                                  aGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGAATGATTCggg                                               >  1:621236/1‑150 (MQ=255)
                                                                                                    gTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGAATGATTCGGGTc                                             >  1:1370876/1‑150 (MQ=255)
                                                                                                     tAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGAATGATTCGGGTCg                                            <  2:4696316/150‑1 (MQ=255)
                                                                                                      aaaCCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGAATGATTCGGGTCGa                                           <  1:1731660/150‑1 (MQ=255)
                                                                                                        aCCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGAATGATTCGGGTCGATc                                         <  2:2038859/150‑1 (MQ=255)
                                                                                                          cGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGAATGATTCGGGTCGATCAg                                       >  1:2377039/1‑150 (MQ=255)
                                                                                                          cGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGAATGATTCGGGTCGATCAg                                       <  1:3817399/150‑1 (MQ=255)
                                                                                                          cGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGAATGATTCGGGTCGATCAg                                       <  1:477201/150‑1 (MQ=255)
                                                                                                           ggCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGAATGATTCGGGTCGATCAGa                                      >  1:4129046/1‑150 (MQ=255)
                                                                                                              atatCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGAATGATTCGGGTCGATCAGACct                                   <  1:4746848/150‑1 (MQ=255)
                                                                                                               tatCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGACTGATTCGGGTCGATCAGACctt                                  >  2:1789033/1‑150 (MQ=255)
                                                                                                               tatCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGAATGATTCGGGTCGATCAGACctt                                  >  2:1268895/1‑150 (MQ=255)
                                                                                                               tatCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGAATGATTCGGGTCGATCAGACctt                                  <  1:1233762/150‑1 (MQ=255)
                                                                                                                atCACCCACAATCTGTTTCACTTGGGCGATAACGTCATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGAATGATTCGGGTCGATCAGACcttc                                 >  1:91797/1‑150 (MQ=255)
                                                                                                                   aCCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGAATGATTCGGGTCGATCAGACCTTCttt                              <  2:1874024/150‑1 (MQ=255)
                                                                                                                   aCCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGAATGATTCGGGTCGATCAGACCTTCttt                              <  2:219714/150‑1 (MQ=255)
                                                                                                                     ccACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGAATGATTCGGGTCGATCAGACCTTCTTTCg                            <  2:650318/150‑1 (MQ=255)
                                                                                                                      cacaATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGAATGATTCGGGTCGATCAGACCTTCTTTCgg                           <  2:2979268/150‑1 (MQ=255)
                                                                                                                      cacaATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGAATGATTCGGGTCGATCAGACCTTCTTTCgg                           <  1:2068386/150‑1 (MQ=255)
                                                                                                                       acaATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGAATGATTCGGGTCGATCAGACCTTCTTTCGgc                          <  2:5049425/150‑1 (MQ=255)
                                                                                                                          aTCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGAATGATTCGGGTCGATCAGACCTTCTTTCGGcgcg                       <  2:2803980/150‑1 (MQ=255)
                                                                                                                             tGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGAATGATTCGGGTCGATCAGACCTTCTTTCGGCGCGGta                    >  2:4767322/1‑150 (MQ=255)
                                                                                                                              gTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGAATGATTCGGGTCGATCAGACCTTCTTTCGGCGCGGtat                   <  2:2506185/150‑1 (MQ=255)
                                                                                                                              gTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGAATGATTCGGGTCGATCAGACCTTCTTTCGGCGCGGtat                   <  2:1020242/150‑1 (MQ=255)
                                                                                                                                    cTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGAATGATTCGGGTCGATCAGACCTTCTTTCGGCGCGGTATAGAACa             >  2:1145930/1‑150 (MQ=255)
                                                                                                                                      tGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGAATGATTCGGGTCGATCAGACCTTCTTTCGGCGCGGTATAGAACATa           <  2:3593334/150‑1 (MQ=255)
                                                                                                                                      tGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGAATGATTCGGGTCGATCAGACCTTCTTTCGGCGCGGTATAGAACATa           >  2:662937/1‑150 (MQ=255)
                                                                                                                                         gCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGACTGATTCGGGTCGATCAGACCTTCTTTCGGCGCGGTATAGAACATAGTg        >  1:610528/1‑150 (MQ=255)
                                                                                                                                           gATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGAATGATTCGGGTCGATCAGACCTTCTTTCGGCGCGGTATAGAACATAGTGcc      >  2:3868494/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                 
GTACCAGTTTAATAGCGCGATCGGAGGTCAGGCCGCCAATCACTGGCGTACCGGTGCCTGGTGCAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTCATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGAATGATTCGGGTCGATCAGACCTTCTTTCGGCGCGGTATAGAACATAGTGCCGTGG  >  CP009273/3076379‑3076671

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑