Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,132,601 T→G 25.7% I172M (ATT→ATG)  yghU → putative S‑transferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,132,6010TG25.7% 500.6 / 282.0 315I172M (ATT→ATG) yghUputative S‑transferase
Reads supporting (aligned to +/- strand):  ref base T (130/104);  new base G (41/40);  total (171/144)
Fisher's exact test for biased strand distribution p-value = 5.18e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.42e-01

Only 100 of 316 total aligned reads displayed.

TTATCTGGCGGAGAAATTTGGCTACTTCCTGCCGCAGGATTTGGCAAAGCGTACTGAAACGATGAACTGGCTGTTCTGGTTACAGGGCGCGGCACCGTTCCTCGGCGGTGGTTTTGGTCACTTTTACCATTACGCACCGGTAAAGATTGAGTACGCCATCAACCGCTTTACCATGGAAGCCAAACGTCTGCTCGACGTGCTGGATAAGCAACTGGCGCAGCATAAGTTTGTTGCGGGCGATGAGTACACCATTGCGGATATGGCGATTTGGCCGTGGTTTGGCAACGTGGTGTTAGG  >  CP009273/3132454‑3132750
                                                                                                                                                   |                                                                                                                                                     
 tATCTGGCGGAGAAATTTGGCTACTTCCTGCCGCAGGATTTGGCAAAGCGTACTGAAACGATGAACTGGCTGTTCTGGTTACAGGGCGCGGCACCGTTCCTCGGCGGTGGTTTTGGTCACTTTTACCATTACGCACCGGTAAAGATGGAg                                                                                                                                                    >  1:3387776/1‑150 (MQ=255)
  aTCTGGCGGAGAAATTTGGCTACTTCCTGCCGCAGGATTTGGCAAAGCGTACTGAAACGATGAACTGGCTGTTCTGGTTACAGGGCGCGGCACCGTTCCTCGGCGGTGGTTTTGGTCACTTTTACCATTACGCACCGGTAAAGATTGAGt                                                                                                                                                   >  1:1626795/1‑150 (MQ=255)
  aTCTGGCGGAGAAATTTGGCTACTTCCTGCCGCAGGATTTGGCAAAGCGTACTGAAACGATGAACTGGCTGTTCTGGTTACAGGGCGCGGCACCGTTCCTCGGCGGTGGTTTTGGTCACTTTTACCATTACGCACCGGTAAAGATGGAGt                                                                                                                                                   >  2:1205933/1‑150 (MQ=255)
    cTGGCGGAGAAATTTGGCTACTTCCTGCCGCAGGATTTGGCAAAGCGTACTGAAACGATGAACTGGCTGTTCTGGTTACAGGGCGCGGCACCGTTCCTCGGCGGTGGTTTTGGTCACTTTTACCATTACGCACCGGTAAAGATGGAGTAc                                                                                                                                                 <  1:97613/150‑1 (MQ=255)
     tGGCGGAGAAATTTGGCTACTTCCTGCCGCAGGATTTGGCAAAGCGTACTGAAACGATGAACTGGCTGTTCTGGTTACAGGGCGCGGCACCGTTCCTCGGCGGTGGTTTTGGTCACTTTTACCATTACGCACCGGTAAAGATTGAGTACg                                                                                                                                                <  2:3428005/150‑1 (MQ=255)
     tGGCGGAGAAATTTGGCTACTTCCTGCCGCAGGATTTGGCAAAGCGTACTGAAACGATGAACTGGCACTTCTGGTTACAGGGCGCGGCACCGTTCCTCGGCGGTGGTTTTGGTCACTTTTACCATTACGCACCGGTAAAGATTGAGTACg                                                                                                                                                <  1:4700239/150‑1 (MQ=255)
         ggAGAAATTTGGCTACTTCCTGCCGCAGGATTTGGCAAAGCGTACTGAAACGATGAACTGGCTGTTCTGGTTACAGGGCGCGGCACCGTTCCTCGGCGGTGGTTTTGGTCACTTTTACCATTACGCACCGGTAAAGATTGAGTACGCCAt                                                                                                                                            >  2:4149098/1‑150 (MQ=255)
         ggAGAAATTTGGCTACTTCCTGCCGCAGGATTTGGCAAAGCGTACTGAAACGATGAACTGGCTGTTCTGGTTACAGGGCGCGGCACCGTTCCTCGGCGGTGGTGTTGGTCACTTTTACCATTACGCACCGGTCAAGATTGAGTACGCCAt                                                                                                                                            >  2:3666793/1‑150 (MQ=255)
          gagaAATTTGGCTACTTCCTGCCGCAGGATTTGGCAAAGCGTACTGAAACGATGAACTGGCTGTTCTGGTTACAGGGCGCGGCACCGTTCCTCGGCGGTGGTTTTGGTCACTTTTACCATTACGCACCGGTAAAGATTGAGTACGCCATc                                                                                                                                           <  2:377852/150‑1 (MQ=255)
           agaAATTTGGCTACTTCCTGCCGCAGGATTTGGCAAAGCGTACTGAAACGATGAACTGGCTGTTCTGGTTACAGGGCGCGGCACCGTTCCTCGGCGGTGGTTTTGGTCACTTTTACCATTACGCACCGGTAAAGATGGAGTACGCCATCa                                                                                                                                          <  2:258608/150‑1 (MQ=255)
            gaAATTTGGCTACTTCCTGCCGCAGGATTTGGCAAAGCGTACTGAAACGATGAACTGGCTGTTCTGGTTACAGGGCGCGGCACCGTTCCTCGGCGGTGGTGTTGGTCACTTTTACCATTACGCACCGGTAAAGATTGAGTACGCCATCaa                                                                                                                                         >  2:531764/1‑150 (MQ=255)
               aTTTGGCTACTTCCTGCCGCAGGATTTGGCAAAGCGTACTGAAACGATGAACTGGCTGTTCTGGTTACAGGGCGCGGCACCGTTCCTCGGCGGTGGTTTTGGTCACTTTTACCATTACGCACCGGTAAAGATTGAGTACGCCATCAACCg                                                                                                                                      <  1:65012/150‑1 (MQ=255)
                 ttGGCTACTTCCTGCCGCAGGATTTGGCAAAGCGTACTGAAACGATGAACTGGCTGTTCTGGTTACAGGGCGCGGCACCGTTCCTCGGCGGTGGTTTTGGTCACTTTTACCATTACGCACCGGTAAAGATTGAGTACGCCATCAACCGCt                                                                                                                                    <  2:2392400/150‑1 (MQ=255)
                  tGGCTACTTCCTGCCGCAGGATTTGGCAAAGCGTACTGAAACGATGAACTGGCTGTTCTGGTTACAGGGCGCGGCACCGTTCCTCGGCGGTGGTTTTGGTCACTTTTACCATTACGCACCGGTAAAGATTGAGTACGCCATCAACCGCtt                                                                                                                                   <  1:2182586/150‑1 (MQ=255)
                  tGGCTACTTCCTGCCGCAGGATTTGGCAAAGCGTACTGAAACGATGAACTGGCTGTTCTGGTTACAGGGCGCGGCACCGTTCCTCGGCGGTGGTTTTGGTCACTTTTACCATTACGCACCGGTAAAGATGGAGTACGCCATCAACCGCtt                                                                                                                                   >  1:2511758/1‑150 (MQ=255)
                    gCTACTTCCTGCCGCAGGATTTGGCAAAGCGTACTGAAACGATGAACTGGCTGTTCTGGTTACAGGGCGCGGCACCGTTCCTCGGCGGTGGTTTTGGTCACTTTTACCATTACGCACCGGTAAAGATTGAGTACGCCATCAACCGCTTTa                                                                                                                                 <  1:2919152/150‑1 (MQ=255)
                     cTACTTCCTGCCGCAGGATTTGGCAAAGCGTACTGAAACGATGAACTGGCTGTTCTGGTTACAGGGCGCGGCACCGTTCCTCGGCGGTGGTTTTGGTCACTTTTACCATTACGCACCGGTAAAGATTGAGTACGCCATCAACCGCTTTAc                                                                                                                                <  2:1702296/150‑1 (MQ=255)
                       aCTTCCTGCCGCAGGATTTGGCAAAGCGTACTGAAACGATGAACTGGCTGTTCTGGTTACAGGGCGCGGCACCGTTCCTCGGCGGTGGTTTTGGTCACTTTTACCATTACGCACCGGTAAAGATTGAGTACGCCATCAACCGCTTTACCa                                                                                                                              >  2:5122353/1‑150 (MQ=255)
                         ttCCTGCCGCAGGATTTGGCAAAGCGTACTGAAACGATGAACTGGCTGTTCTGGTTACAGGGCGCGGCACCGTTCCTCGGCGGTGGTTTTGGTCACTTTTACCATTACGCACCGGTAAAGATTGAGTACGCCATCAACCGCTTTACCATg                                                                                                                            >  2:2338235/1‑150 (MQ=255)
                          tCCTGCCGCAGGATTTGGCAAAGCGTACTGAAACGATGAACTGGCTGTTCTGGTTACAGGGCGCGGCACCGTTCCTCGGCGGTGGTTTTGGTCACTTTTACCATTACGCACCGGTAAAGATTGAGTACGCCATCAACCGCTTTACCATgg                                                                                                                           >  2:2914116/1‑150 (MQ=255)
                            cTGCCGCAGGATTTGGCAAAGCGTACTGAAACGATGAACTGGCTGTTCTGGTTACCGGGCGCGGCACCGTTCCTCGGCGGTGGTTTTGGTCACTTTTACCATTACGCACCGGTAAAGATTGAGTACGCCATCAACCGCTGTACCATGGaa                                                                                                                         >  1:3103618/1‑150 (MQ=255)
                               ccGCAGGATTTGGCAAAGCGTACTGAAACGATGAACTGGCTGTTCTGGTTACAGGGCGCGGCACCGTTCCTCGGCGGTGGTTTTGGTCACTTTTACCATTACGCACCGGTAAAGATTGAGTACGCCATCAACCGCTTTACCATGGAAGcc                                                                                                                      >  2:2930255/1‑150 (MQ=255)
                               ccGCAGGATTTGGCAAAGCGTACTGAAACGATGAACTGGCTGTTCTGGTTACAGGGCGCGGCACCGTTCCTCGGCGGTGGTTTTGGTCACTTTTACCATTACGCACCGGTAAAGATTGAGTACGCCATCAACCGCTTTACCATGGAAGcc                                                                                                                      <  1:853485/150‑1 (MQ=255)
                                cGCAGGATTTGGCAAAGCGTACTGAAACGATGAACTGGCTGTTCTGGTTACAGGGCGCGGCACCGTTCCTCGGCGGTGGTTTTGGTCACTTTTACCATTACGCACCGGTAAAGATGGAGTACGCCATCAACCGCTTTACCATGGAAGCCa                                                                                                                     >  2:4220603/1‑150 (MQ=255)
                                 gCAGGATTTGGCAAAGCGTACTGAAACGATGAACTGGCTGTTCTGGTTACAGGGCGCGGCACCGTTCCTCGGCGGTGGTTTTGGTCACTTTTACCATTACGCACCGGTAAAGATTGAGTACGCCATCAACCGCTTTACCATGGAAGCCaa                                                                                                                    >  2:3074339/1‑150 (MQ=255)
                                   aGGATTTGGCAAAGCGTACTGAAACGATGAACTGGCTGTTCTGGTTACAGGGCGCGGCACCGTTCCTCGGCGGTGGTTTTGGTCACTTTTACCATTACGCACCGGTAAAGATTGAGTACGCCATCAACCGCTTTACCATGGAAGCCAAAc                                                                                                                  >  1:805580/1‑150 (MQ=255)
                                    ggATTTGGCAAAGCGTACTGAACCGATGAACTGGCTGTTCTGGTTACAGGGCGCGGCACCGTTCCTCGGCGGTGGTTTTGGTCACTTTTACCATTACGCACCGGTAAAGATTGAGTACGCCATCAACCGCTTTACCATGGAAGCCAAACg                                                                                                                 <  1:2541763/150‑1 (MQ=255)
                                    ggATTTGGCAAAGCGTACTGAAACGATGAACTGGCTGTTCTGGTTACAGGGCGCGGCACCGTTCCTCGGCGGTGGTTTTGGTCACTTTTACCATTACGCACCGGTAAAGATTGAGTACGCCATCAACCGCTTTACCATGGAAGCCAAACg                                                                                                                 >  1:1600157/1‑150 (MQ=255)
                                     gATTTGGCAAAGCGTACTGAAACGATGAACGGGCTGTTCTGGTTACAGGGCGCGGCACCGTTCCTCGGCGGTGGTTTTGGTCACTTTTACCATTACGCACCGGTAAAGATGGAGTACGCCATCAACCGCTTTACCATGGAAGCCAAACGt                                                                                                                <  2:4697239/150‑1 (MQ=255)
                                           gCAAAGCGTACTGAAACGATGAACTGGCTGTTCTGGTTACAGGGCGCGGCACCGTTCCTCGGCGGTGGTTTTGGTCACTTTTACCATTACGCACCGGTAAAGATTGAGTACGCCATCAACCGCTTTACCATGGAAGCCAAACGTCTGCTc                                                                                                          <  2:2012922/150‑1 (MQ=255)
                                            cAAAGCGTACTGAAACGATGAACTGGCTGTTCTGGTTACAGGGCGCGGCACCGTTCCTCGGCGGTGGTTTTGGTCACTTTTACCATTACGCACCGGTAAAGATTGAGTACGCCATCAACCGCTTTACCATGGAAGCCAAACGTCTGCTCg                                                                                                         <  2:3368932/150‑1 (MQ=255)
                                                gCGTACTGAAACGATGAACTGGCTGTTCTGGTTACAGGGCGCGGCACCGTTCCTCGGCGGTGGTTTTGGTCACTTTTACCATTACGCACCGGTAAAGATTGAGTACGCCATCAACCGCTTTACCATGGAAGCCAAACGTCTGCTCGACGt                                                                                                     <  1:2814851/150‑1 (MQ=255)
                                                 cGTACTGAAACGATGAACTGGCTGTTCTGGTTACAGGGCGCGGCACCGTTCCTCGGCGGTGGTTTTGGTCACTTTTACCATTACGCACCGGTATAGAGTGAGTACGCCATCTACCGCTGTCCCATGGAAGCCAGACGGCTGCTCGTCGTg                                                                                                    >  2:2678701/1‑150 (MQ=255)
                                                 cGTACTGAAACGATGAACTGGCTGTTCTGGTTACAGGGCGCGGCACCGTTCCTCGGCGGTGGTTTTGGTCACTTTTACCATTACGCACCGGTAAAGATGGAGTACGCCATCAACCGCTTTACCATGGAAGCCAAACGTCTGCTCGACGTg                                                                                                    <  2:282700/150‑1 (MQ=255)
                                                   tACTGAAACGATGAACTGGCTGTTCTGGTTACAGGGCGCGGCACCGTTCCTCGGCGGTGGTTTTGGTCACTTTTACCATTACGCACCGGTAAAGATTGAGTACGCCATCAACCGCTTTACCATGGAAGCCAAACGTCTGCTCGACGTGCt                                                                                                  <  2:3989683/150‑1 (MQ=255)
                                                    aCTGAAACGATGAACTGGCTGTTCTGGTTACAGGGCGCGGCACCGTTCCTCGGCGGTGGTTTTGGTCACTTTTACCATTACGCACCGGTAAAGATTGAGTACGCCATCAACCGCTTTACCATGGAAGCCAAACGTCTGCTCGACGTGCTg                                                                                                 >  2:643449/1‑150 (MQ=255)
                                                    aCTGAAACGATGAACTGGCTGTTCTGGTTACAGGGCGCGGCACCGTTCCTCGGCGGTGGTTTTGGTCACTTTTACCATTACGCACCGGTAAAGATGGAGTACGCCATCAACCGCTTTACCATGGAAGCCAAACGTCTGCTCGACGTGCTg                                                                                                 <  2:3797645/150‑1 (MQ=255)
                                                      tGAAACGATGAACTGGCTGTTCTGGTTACAGGGCGCGGCACCGTTCCTCGGCGGTGGTTTTGGTCACTTTTACCATTACGCACCGGTAAAGATTGAGTACGCCATCAACCGCTTTACCATGGAAGCCAAACGTCTGCTCGACGTGCTGGa                                                                                               >  2:4073464/1‑150 (MQ=255)
                                                       gAAACGATGAACTGGCTGTTCTGGTTACAGGGCGCGGCACCGTTCCTCGGCGGTGGTTTTGGTCACTTTTACCATTACGCACCGGTAAAGATTGAGTACGCCATCAACCGCTTTACCATGGAAGCCAAACGTCTGCTCGACGTGCTGGAt                                                                                              <  1:37129/150‑1 (MQ=255)
                                                         aaCGATGACCTGGCTGTTCTGGTTACAGGGCGCGGCACCGTTCCTCGGCGGTGGTTTTGGTCACTTTTACCATTACGCACCGGTAAAGATTGAGTACGCCATCAACCGCTTTACCATGGACGCCAAACGTCTGCTCGACGTGCTGGATaa                                                                                            >  2:3856725/1‑150 (MQ=255)
                                                         aaCGATGAACTGGCTGTTCTGGTTACAGGGCGCGGCACCGTTCCTCGGCGGTGGTTTTGGTCACTTTTACCATTACGCACCGGTAAAGATTGAGTACGCCATCAACCGCTTTACCATGGAAGCCAAACGTCTGCTCGACGTGCTGGATaa                                                                                            >  2:1395952/1‑150 (MQ=255)
                                                           cGATGAACTGGCTGTTCTGGTTACAGGGCGCGGCACCGTTCCTCGGCGGTGGTTTTGGTCACTTTTACCATTACGCACCGGTAAAGATTGAGTACGCCATCAACCGCTTTACCATGGAAGCCAAACGTCTGCTCGACGTGCTGGATAAGc                                                                                          >  2:1756204/1‑150 (MQ=255)
                                                           cGATGAACTGGCTGTTCTGGTTACAGGGCGCGGCACCGTTCCTCGGCGGTGGTTTTGGTCACTTTTACCATTACGCACCGGTAAAGATTGAGTACGCCATCAACCGCTTTACCATGGAAGCCAAACGTCTGCTCGACGTGCTGGATAAGc                                                                                          <  1:712603/150‑1 (MQ=255)
                                                             aTGAACTGGCTGTTCTGGTTACAGGGCGCGGCACCGTTCCTCGGCGGTGGTTTTGGTCACTTTTACCATTACGCACCGGTAAAGATTGAGTACGCCATCAACCGCTTTACCATGGAAGCCAAACGTCTGCTCGACGTGCTGGATAAGCaa                                                                                        >  2:3899822/1‑150 (MQ=255)
                                                               gAACTGGCTGTTCTGGTTACAGGGCGCGGCACCGTTCCTCGGCGGTGGTTTTGGTCACTTTTACCATTACGCACCGGTAAAGATTGAGTACGCCATCAACCGCTTTACCATGGAAGCCAAACGTCTGCTCGACGTGCTGGATAAGCAACt                                                                                      >  1:903942/1‑150 (MQ=255)
                                                                aaCTGGCTGTTCTGGTTACAGGGCGCGGCACCGTTCCTCGGCGGTGGTTTTGGTCACTTTTACCATTACGCACCGGTAAAGATTGAGTACGCCATCAACCGCTTTACCATGGAAGCCAAACGTCTGCTCGACGTGCTGGATAAGCAACTg                                                                                     <  1:2487166/150‑1 (MQ=255)
                                                                aaCTGGCTGTTCTGGTTACAGGGCGCGGCACCGTTCCTCGGCGGTGGTTTTGGTCACTTTTACCATTACGCACCGGTAAAGATTGAGTACGCCATCAACCGCTTTACCATGGAAGCCAAACGTCTGCTCGACGTGCTGGATAAGCAACTg                                                                                     <  1:3931416/150‑1 (MQ=255)
                                                                  cTGGCTGTTCTGGTTACAGGGCGCGGCACCGTTCCTCGGCGGTGGTTTTGGTCACTTTTACCATTACGCACCGGTAAAGATTGAGTACGCCATCAACCGCTTTACCATGGAAGCCAAACGTCTGCTCGACGTGCTGGATAAGCAACTGgc                                                                                   <  1:1001889/150‑1 (MQ=255)
                                                                   tGGCTGTTCTGGTTACAGGGCGCGGCACCGTTCCTCGGCGGTGGTTTTGGTCACTTTTACCATTACGCACCGGTAAAGATTGAGTACGCCATCAACCGCTTTACCATGGAAGCCAAACGTCTGCTCGACGTGCTGGATAAGCAACTGgcg                                                                                  <  1:2626060/150‑1 (MQ=255)
                                                                   tGGCTGTTCTGGTTACAGGGCGCGGCACCGTTCCTCGGCGGTGGTTTTGGTCACTTTTACCATTACGCACCGGTAAAGATGGAGTACGCCATCAACCGCTTTACCATGGAAGCCAAACGTCTGCTCGACGTGCTGGATAAGCAACTGgcg                                                                                  <  1:507976/150‑1 (MQ=255)
                                                                     gCTGTTCTGGTTACAGGGCGCGGCACCGTTCCTCGGCGGTGGTTTTGGTCACTTTTACCATTACGCACCGGTAAAGATTGAGTACGCCATCAACCGCTTTACCATGGAAGCCAAACGTCTGCTCGACGTGCTGGATAAGCAACTGGCgca                                                                                >  2:2637246/1‑150 (MQ=255)
                                                                        gTTCTGGTTACAGGGCGCGGCACCGTTCCTCGGCGGTGGTTTTGGTCACTTTTACCATTACGCACCGGTAAAGATGGAGTACGCCATCAACCGCTTTACCATGGAAGCCAAACGTCTGCTCGACGTGCTGGATAAGCAACTGGCgcagca                                                                             >  1:5094197/1‑150 (MQ=255)
                                                                         ttCTGGTTACAGGGCGCGGCACCGTTCCTCGGCGGTGGTTTTGGTCACTTTTACCATTACGCACCGGTAAAGATGGAGTACGCCATCAACCGCTTTACCATGGAAGCCAAACGTCTGCTCGACGTGCTGGATAAGCAACTGGCGCAGCAt                                                                            <  2:3540808/150‑1 (MQ=255)
                                                                          tCTGGTTACAGGGCGCGGCACCGTTCCTCGGCGGTGGTTTTGGTCACTTTTACCATTACGCACCGGTAAAGATTGAGTACGCCATCAACCGCTTTACCATGGAAGCCAAACGTCTGCTCGACGTGCTGGATAAGCAACTGGCGCAGCATa                                                                           <  2:1517672/150‑1 (MQ=255)
                                                                           cTGGTTACAGGGCGCGGCACCGTTCCTCGGCGGTGGTTTTGGTCACTTTTACCATTACGCACCGGTAAAGATTGAGTACGCCATCAACCGCTTTACCATGGAAGCCAAACGTCTGCTCGACGTGCTGGATAAGCAACTGGCGCAGCATaa                                                                          >  2:2733471/1‑150 (MQ=255)
                                                                             ggTTACAGGGCGCGGCACCGTTCCTCGGCGGTGGTTTTGGTCACTTTTACCATTACGCACCGGTAAAGATGGAGTACGCCATCAACCGCTTTACCATGGAAGCCAAACGTCTGCTCGACGTGCTGGATAAGCAACTGGCGCAGCATAAGt                                                                        >  1:2058091/1‑150 (MQ=255)
                                                                              gTTACAGGGCGCGGCACCGTTCCTCGGCGGTGGTTTTGGTCACTTTTACCATTACGCACCGGTAAAGATTGAGTACGCCATCAACCGCTTTACCATGGAAGCCAAACGTCTGCTCGACGTGCTGGATAAGCAACTGGCGCAGCATAAGtt                                                                       <  2:575552/150‑1 (MQ=255)
                                                                              gTTACAGGGCGCGGCACCGTTCCTCGGCGGTGGTTTTGGTCACTTTTACCATTACGCACCGGTAAAGATTGAGTACGCCATCAACCGCTTTACCATGGAAGCCAAACGTCTGCTCGACGTGCTGGATAAGCAACTGGCGCAGCATAAGtt                                                                       <  2:4992025/150‑1 (MQ=255)
                                                                               ttACAGGGCGCGGCACCGTTCCTCGGCGGTGGTTTTGGTCACTTTTACCATTACGCACCGGTAAAGATTGAGTACGCCATCAACCGCTTTACCATGGAAGCCAAACGTCTGCTCGACGTGCTGGATAAGCAACTGGCGCAGCATAAGttt                                                                      <  2:2150069/150‑1 (MQ=255)
                                                                               ttACAGGGCGCGGCACCGTTCCTCGGCGGTGGTTTTGGTCACTTTTACCATTACGCACCGGTAAAGATTGAGTACGCCATCAACCGCTTTACCATGGAAGCCAAACGTCTGCTCGACGTGCTGGATAAGCAACTGGCGCAGCATAAGttt                                                                      <  2:4526149/150‑1 (MQ=255)
                                                                                   aGGGCGCGGCACCGTTCCTCGGCGGTGGTTTTGGTCACTTTTACCATTACGCACCGGTAAAGATTGAGTACGCCATCAACCGCTTTACCATGGAAGCCAAACGTCTGCTCGACGTGCTGGATAAGCAACTGGCGCAGCATAAGTttgttg                                                                  >  2:4628679/1‑150 (MQ=255)
                                                                                    gggCGCGGCACCGTTCCTCGGCGGTGGTTTTGGTCACTTTTACCATTACGCACCGGTAAAGATTGAGTACGCCATCAACCGCTTTACCATGGAAGCCAAACGTCTGCTCGACGTGCTGGATAAGCAACTGGCGCAGCATAAGTTTGTTGc                                                                 >  2:794795/1‑150 (MQ=255)
                                                                                         cgGCACCGTTCCTCGGCGGTGGTTTTGGTCACTTTTACCATTACGCACCGGTAAAGATGGAGTACGCCATCAACCGCTTTACCATGGAAGCCAAACGTCTGCTCGACGTGCTGGATAAGCAACTGGCGCAGCATAAGTTTGTTGCGGGCg                                                            >  1:4485835/1‑150 (MQ=255)
                                                                                          ggCACCGTTCCTCGGCGGTGGTTTTGGTCACTTTTACCATTACGCACCGGTAAAGATGGAGTACGCCATCAACCGCTTTACCATGGAAGCCAAACGTCTGCTCGACGTGCTGGATAAGCAACTGGCGCAGCATAAGTTTGTTGCGGGCGa                                                           >  2:1079232/1‑150 (MQ=255)
                                                                                             aCCGTTCCTCGGCGGTGGTTTTGGTCACTTTTACCATTACGCACCGGTAAAGATTGAGTACGCCATCAACCGCTTTACCATGGAAGCCAAACGTCTGCTCGACGTGCTGGATAAGCAACTGGCGCAGCATAAGTTTGTTGCGGGCGATGa                                                        >  2:2259212/1‑150 (MQ=255)
                                                                                              ccGTTCCTCGGCGGTGGTTTTGGTCACTTTTACCATTACGCACCGGTAAAGATTGAGTACGCCATCAACCGCTTTACCATGGAAGCCAAACGTCTGCTCGACGTGCTGGATAAGCAACTGGCGCAGCATAAGTTTGTTGCGGGCGATGAg                                                       >  1:4763407/1‑150 (MQ=255)
                                                                                              ccGGTCCTCGGCGGTGGTTTTGGTCACTTTTACCATTACGCACCGGTAAAGATTGAGTACGCCATCAACCGCTTTACCATGGAAGCCAAACGTCTGCTCGACGTGCTGGATAAGCAACTGGCGCAGCATAAGTTTGTTGCGGGCGATGAg                                                       <  1:3999544/150‑1 (MQ=255)
                                                                                                 gtCCTCGGCGGTGGTTTTGGTCACTTTGACCAGTACGCACCGGTAAAGATGGAGTCCGCCATCAACCGCTTTACCATGGCAGCCACACGTCTGCTCGACGTGCTGGAGAAGCAACTGGCGCAGCATAAGTTTGTTGCGGGCGATGAGTac                                                    <  2:1227608/149‑1 (MQ=255)
                                                                                                  tCCTCGGCGGTGGTTTTGGTCACTTTTACCATTACGCACCGGTAAAGATTGAGTACGCCATCAACCGCTTTACCATGGAAGCCAAACGTCTGCTCGACGTGCTGGATAAGCAACTGGCGCAGCATAAGTTTGTTGCGGGCGATGAGTaca                                                   <  1:1376657/150‑1 (MQ=255)
                                                                                                   ccTCGGCGGTGGTTTTGGTCACTTTTACCATTACGCACCGGTAAAGATTGAGTACGCCATCAACCGCTTTACCATGGAAGCCAAACGTCTGCTCGACGTGCTGGATAAGCAACTGGCGCAGCATAAGTTTGTTGCGGGCGATGAGTacac                                                  <  1:3180820/150‑1 (MQ=255)
                                                                                                   ccTCGGCGGTGGTTTTGGTCACTTTTACCATTACGCACCGGTAAAGATGGAGTACGCCATCAACCGCTTTACCATGGAAGCCAAACGTCTGCTCGACGTGCTGGATAAGCAACTGGCGCAGCATAAGTTTGTTGCGGGCGATGAGTacac                                                  <  1:2748604/150‑1 (MQ=255)
                                                                                                    cTCGGCGGTGGTTTTGGTCACTTTTACCATTACGCACCGGTAAAGATTGAGTACGCCATCAACCGCTTTACCATGGAAGCCAAACGTCTGCTCGACGTGCTGGATAAGCAACTGGCGCAGCATAAGTTTGTTGCGGGCGATGAGTACAcc                                                 >  2:4508531/1‑150 (MQ=255)
                                                                                                     tCGGCGGGGGTTTTGGTCACTTTTACCATTACGCACCGGTAAAGATGGAGTACGCCATCAACCGCTTTACCATGGAAGCCAAACGTCTGCTCGACGTGCTGGATAAGCAACTGGCGCAGCATAAGTTTGTTGCGGGCGATGAGTACAccc                                                >  2:3964402/1‑149 (MQ=255)
                                                                                                      cggcggTGGTTTTGGTCACTTTTACCATTACGCACCGGTAAAGATGGAGTACGCCATCAACCGCTTTACCATGGAAGCCAAACGTCTGCTCGACGTGCTGGATAAGCAACTGGCGCAGCATAAGTTTGTTGCGGGCGATGAGTACACCAt                                               >  2:4366927/1‑150 (MQ=255)
                                                                                                         cggTGGTTTTGGTCACTTTTACCATTACGCACCGGTAAAGATGGAGTACGCCATCAACCGCTTTACCATGGAAGCCAAACGTCTGCTCGACGTGCTGGATAAGCAACTGGCGCAGCATAAGTTTGTTGCGGGCGATGAGTACACCATTGc                                            <  1:155818/150‑1 (MQ=255)
                                                                                                          ggtggtTTTGGTCACTTTTACCATTACGCACCGGTAAAGATTGAGTACGCCATCAACCGCTTTACCATGGAAGCCAAACGTCTGCTCGACGTGCTGGATAAGCAACTGGCGCAGCATAAGTTTGTTGCGGGCGATGAGTACACCATTGCg                                           >  1:3641068/1‑150 (MQ=255)
                                                                                                          ggtggtTTTGGTCACTTTTACCATTACGCACCGGTAAAGATGGAGTACGCCATCAACCGCTTTACCATGGAAGCCAAACGTCTGCTCGACGTGCTGGATAAGCAACTGGCGCAGCATAAGTTTGTTGCGGGCGATGAGTACACCATTGCg                                           <  1:4961388/150‑1 (MQ=255)
                                                                                                           gtggtTTTGGTCACTTTTACCATTACGCACCGGTAAAGATTGAGTACGCCATCAACCGCTTTACCATGGAAGCCAAACGTCTGCTCGACGTGCTGGATAAGCAACTGGCGCAGCATAAGTTTGTTGCGGGCGATGAGTACACCATTGCgg                                          >  2:1157014/1‑150 (MQ=255)
                                                                                                            tggtTTTGGTCACTTTTACCATTACGCACCGGTAAAGATTGAGTACGCCATCAACCGCTTTACCATGGAAGCCAAACGTCTGCTCGACGTGCTGGATAAGCAACTGGCGCAGCATAAGTTTGTTGCGGGCGATGAGTACACCATTGCGGa                                         >  1:3616889/1‑150 (MQ=255)
                                                                                                             ggtTTTGGTCACTTTTACCATTACGCACCGGTAAAGATGGAGTACGCCATCAACCGCTTTACCATGGAAGCCAAACGTCTGCTCGACGTGCTGGATAAGCAACTGGCGCAGCATAAGTTTGTTGCGGGCGATGAGTACACCATTGCGGat                                        <  1:1936430/150‑1 (MQ=255)
                                                                                                              gtTTTGGTCACTTTTACCATTACGCACCGGTAAAGATGGAGTACGCCATCAACCGCTTTACCATGGAAGCCAAACGTCTGCTCGACGTGCTGGATAAGCAACTGGCGCAGCATAAGTTTGTTGCGGGCGATGAGTACACCATTGCGGata                                       >  2:2721747/1‑150 (MQ=255)
                                                                                                                tttGGTCACTTTTACCATTACGCACCGGTAAAGATTGAGTACGCCATCAACCGCTTTACCATGGAAGCCAAACGTCTGCTCGACGTGCTGGATAAGCAACTGGCGCAGCATAAGTTTGTTGCGGGCGATGAGTACACCATTGCGGATATg                                     <  1:787602/150‑1 (MQ=255)
                                                                                                                     tCACTTTTACCATTACGCACCGGTAAAGATGGAGTACGCCATCAACCGCTTTACCATGGAAGCCAAACGTCTGCTCGACGTGCTGGATAAGCAACTGGCGCAGCATAAGTTTGTTGCGGGCGATGAGTACACCATTGCGGATATGGCGAt                                >  2:1901021/1‑150 (MQ=255)
                                                                                                                           ttACCATTACGCACCGGTAAAGATTGAGTACGCCATCAACCGCTTTACCATGGAAGCCAAACGTCTGCTCGACGTGCTGGATAAGCAACTGGCGCAGCATAAGTTTGTTGCGGGCGATGAGTACACCATTGCGGATATGGCGATTTGGcc                          >  2:1037568/1‑150 (MQ=255)
                                                                                                                             aCCATTACGCACCGGTAAAGATTGAGTACGCCATCAACCGCTTTACCATGGAAGCCAAACGTCTGCTCGACGTGCTGGATAAGCAACTGGCGCAGCATAAGTTTGTTGCGGGCGATGAGTACACCATTGCGGATATGGCGATTTGGCCGt                        >  1:2303899/1‑150 (MQ=255)
                                                                                                                             aCCATTACGCACCGGTAAAGATGGAGTACGCCATCAACCGCTTTACCATGGAAGCCAAACGTCTGCTCGACGTGCTGGATAAGCAACTGGCGCAGCATAAGTTTGTTGCGGGCGATGAGTACACCATTGCGGATATGGCGATTTGGCCGt                        >  1:2710981/1‑150 (MQ=255)
                                                                                                                              ccATTACGCACCGGTAAAGATTGAGTACGCCATCAACCGCTTTACCATGGAAGCCAAACGTCTGCTCGACGTGCTGGATAAGCAACTGGCGCAGCATAAGTTTGTTGCGGGCGATGAGTACACCATTGCGGATATGGCGATTTGGCCGTg                       <  2:3207146/150‑1 (MQ=255)
                                                                                                                              ccATTACGCACCGGTAAAGATGGAGTACGCCATCAACCGCTTTACCATGGAAGCCAAACGTCTGCTCGACGTGCTGGATAAGCAACTGGCGCAGCATAAGTTTGTTGCGGGCGATGAGTACACCATTGCGGATATGGCGATTTGGCCGTg                       >  1:2387597/1‑150 (MQ=255)
                                                                                                                               cATTACGCACCGGTAAAGATTGAGTACGCCATCAACCGCTTTACCATGGAAGCCAAACGTCTGCTCGACGTGCTGGATAAGCAACTGGCGCAGCATAAGTTTGTTGCGGGCGATGAGTACACCATTGCGGATATGGCGATTTGGCCGTgg                      >  1:4211842/1‑150 (MQ=255)
                                                                                                                                aTTACGCACCGGTAAAGATTGAGTACGCCATCAACCGCTTTACCATGGAAGCCAAACGTCTGCTCGACGTGCTGGATAAGCAACTGGCGCAGCATAAGTTTGTTGCGGGCGATGAGTACACCATTGCGGATATGGCGATTTGGCCGTGGt                     <  1:3962627/150‑1 (MQ=255)
                                                                                                                                     gCACCGGTAAAGATTGAGTACGCCATCAACCGCTTTACCATGGAAGCCAAACGTCTGCTCGACGTGCTGGATAAGCAACTGGCGCAGCATAAGTTTGTTGCGGGCGATGAGTACACCATTGCGGATATGGCGATTTGGCCGTGGTTTGGc                >  2:1871185/1‑150 (MQ=255)
                                                                                                                                      cACCGGTAAAGATTGAGTACGCCATCAACCGCTTTACCATGGAAGCCAAACGTCTGCTCGACGTGCTGGATAAGCAACTGGCGCAGCATAAGTTTGTTGCGGGCGATGAGTACACCATTGCGGATATGGCGATTTGGCCGTGGTTTGGCa               <  1:4081373/150‑1 (MQ=255)
                                                                                                                                      cACCGGTAAAGATTGAGTACGCCATCAACCGCTTTACCATGGAAGCCAAACGTCTGCTCGACGTGCTGGATAAGCAACTGGCGCAGCATAAGTTTGTTGCGGGCGATGAGTACACCATTGCGGATATGGCGATTTGGCCGTGGTTTGGCa               >  1:3205128/1‑150 (MQ=255)
                                                                                                                                          ggTAAAGATTGAGTACGCCATCAACCGCTTTACCATGGAAGCCAAACGTCTGCTCGACGTGCTGGATAAGCAACTGGCGCAGCATAAGTTTGTTGCGGGCGATGAGTACACCATTGCGGATATGGCGATTTGGCCGTGGTTTGGCAACgt           <  1:3596653/150‑1 (MQ=255)
                                                                                                                                          ggTAAAGATTGAGTACGCCATCAACCGCTTTACCATGGAAGCCAAACGTCTGCTCGACGTGCTGGATAAGCAACTGGCGCAGCATAAGTTTGTTGCGGGCGATGAGTACACCATTGCGGATATGGCGATTTGGCCGTGGTTTGGCAACgt           >  1:1848083/1‑150 (MQ=255)
                                                                                                                                           gTAAAGATTGAGTACGCCATCAACCGCTTTACCATGGAAGCCAAACGTCTGCTCGACGTGCTGGATAAGCAACTGGCGCAGCATAAGTTTGTTGCGGGCGATGAGTACACCATTGCGGATATGGCGATTTGGCCGTGGTTTGGCAACgtg          >  1:4299045/1‑150 (MQ=255)
                                                                                                                                           gTAAAGATGGAGTACGCCATCAACCGCTTTACCATGGAAGCCAAACGTCTGCTCGACGTGCTGGATAAGCAACTGGCGCAGCATAAGTTTGTTGCGGGCGATGAGTACACCATTGCGGATATGGCGATTTGGCCGTGGTTTGGCAACgtg          <  1:4512389/150‑1 (MQ=255)
                                                                                                                                               aGATTGAGTACGCCATCAACCGCTTTACCATGGAAGCCAAACGTCTGCTCGACGTGCTGGATAAGCAACTGGCGCAGCATAAGTTTGTTGCGGGCGATGAGTACACCATTGCGGATATGGCGATTTGGCCGTGGTTTGGCAACGTGgtgt      >  1:3865754/1‑150 (MQ=255)
                                                                                                                                               aGATTGAGTACGCCATCAACCGCTTTACCATGGAAGCCAAACGTCTGCTCGACGTGCTGGATAAGCAACTGGCGCAGCATAAGTTTGTTGCGGGCGATGAGTACACCATTGCGGATATGGCGATTTGGCCGTGGTTTGGCAACGTGgtgt      >  1:1293754/1‑150 (MQ=255)
                                                                                                                                                 aTTGAGTACGCCATCAACCGCTTTACCATGGAAGCCAAACGTCTGCTCGACGTGCTGGATAAGCAACTGGCGCAGCATAAGTTTGTTGCGGGCGATGAGTACACCATTGCGGATATGGCGATTTGGCCGTGGTTTGGCAACGTGGTGTTa    >  1:174927/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                     
TTATCTGGCGGAGAAATTTGGCTACTTCCTGCCGCAGGATTTGGCAAAGCGTACTGAAACGATGAACTGGCTGTTCTGGTTACAGGGCGCGGCACCGTTCCTCGGCGGTGGTTTTGGTCACTTTTACCATTACGCACCGGTAAAGATTGAGTACGCCATCAACCGCTTTACCATGGAAGCCAAACGTCTGCTCGACGTGCTGGATAAGCAACTGGCGCAGCATAAGTTTGTTGCGGGCGATGAGTACACCATTGCGGATATGGCGATTTGGCCGTGGTTTGGCAACGTGGTGTTAGG  >  CP009273/3132454‑3132750

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑