BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	3,224,028	C→A	7.3%	L594L (CTC→CTA)	ygjK →	alpha‑glucosidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	3,224,028	0	C	A	7.3%	1307.8 / 47.6	343	L594L (CTC→CTA)	ygjK	alpha‑glucosidase
Reads supporting (aligned to +/- strand): ref base C (172/145); new base A (13/13); total (185/158)
Fisher's exact test for biased strand distribution p-value = 6.88e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 349 total aligned reads displayed.

GGCAAACGTAGCGACTGGACGGTGAAATTCGCCGAAAACCGCAGTCAGGACGGAACGTTGCTGGGCTACTCGCTATTGCAGGAGTCGGTGGATCAGGCCAGCTATATGTACAGCGATAACCATTATCTGGCGGAGATGGCAACCATCCTCGGTAAGCCAGAAGAGGCCAAACGCTATCGCCAGTTGGCACAGCAGCTCGCGGACTACATCAACACCTGTATGTTCGACCCGACTACCCAGTTCTACTATGACGTACGTATTGAAGATAAACCACTGGCGAACGGCTGCGCGGGCAAACC  >  CP009273/3223879‑3224177

ggcAAACGTAGCGACTGGACGGTGAAATTCGCCGAAAACCGCAGTCAGGACGGAACGTTGCTGGGCTACTCGCTATTGCAGGAGTCGGTGGATCAGGCCAGCTATATGTACAGCGATAACCATTATCTGGCGGAGATGGCAACCATCCTc                                                                                                                                                       <  1:1723823/150‑1 (MQ=255)

ggcAAACGTAGCGACTGGACGGTGAAATTCGCCGAAAACCGCAGTCAGGACGGAACGTTGCTGGGCTACTCGCTATTGCAGGAGTCGGTGGATCAGGCCAGCTATATGTACAGCGATAACCATTATCTGGCGGAGATGGCAACCATCCTc                                                                                                                                                       <  1:1998427/150‑1 (MQ=255)

ggcAAACGTAGCGACTGGACGGTGAAATTCGCCGAAAACCGCAGTCAGGACGGAACGTTGCTGGGCTACTCGCTATTGCAGGAGTCGGTGGATCAGGCCAGCTATATGTACAGCGATAACCATTATCTGGCGGAGATGGCAACCATCCTc                                                                                                                                                       >  1:1921504/1‑150 (MQ=255)

   aaaCGTAGCGACTGGACGGTGAAATTCGCCGAAAACCGCAGTCAGGACGGAACGTTGCTGGGCTACTCGCTATTGCAGGAGTCGGTGGATCAGGCCAGCTATATGTACAGCGATAACCATTATCTGGCGGAGATGGCAACCATCCTCGGt                                                                                                                                                    <  2:1865029/150‑1 (MQ=255)

   aaaCGTAGCGACTGGACGGTGAAATTCGCCGAAAACCGCAGTCAGGACGGAACGTTGCTGGGCTACTCGCTATTGCAGGAGTCGGTGGATCAGGCCAGCTATATGTACAGCGATAACCATTATCTGGCGGAGATGGCAACCATCCTCGGt                                                                                                                                                    >  1:3910951/1‑150 (MQ=255)

     aCGTAGCGACTGGACGGTGAAATTCGCCGAAAACCGCAGTCAGGACGGAACGTTGCTGGGCTACTCGCTATTGCAGGAGTCGGTGGATCAGGCCAGCTATATGTACAGCGATAACCATTATCTGGCGGAGATGGCAACCATCCTCGGTaa                                                                                                                                                  >  2:923256/1‑150 (MQ=255)

     aCGTAGCGACTGGACGGTGAAATTCGCCGAAAACCGCAGTCAGGACGGAACGTTGCTGGGCTACTCGCTATTGCAGGAGTCGGTGGATCAGGCCAGCTATATGTACAGCGATAACCATTATCTGGCGGAGATGGCAACCATCCTCGGTaa                                                                                                                                                  <  2:2015853/150‑1 (MQ=255)

     aCGTAGCGACTGGACGGTGAAATTCGCCGAAAACCGCAGTCAGGACGGAACGTTGCTGGGCTACTCGCTATTGCAGGAGTCGGTGGATCAGGCCAGCTATATGTACAGCGATAACCATTATCTGGCGGAGATGGCAACCATCCTCGGTaa                                                                                                                                                  <  1:3244854/150‑1 (MQ=255)

        tAGCGACTGGACGGTGAAATTCGCCGAAAACCGCAGTCAGGACGGAACGTTGCTGGGCTACTCGCTATTGCAGGAGTCGGTGGATCAGGCCAGCTATATGTACAGCGATAACCATTATCTGGCGGAGATGGCAACCATCCTCGGTAAGcc                                                                                                                                               <  2:2909501/150‑1 (MQ=255)

            gACTGGACGGTGAAATTCGCCGAAAACCGCAGTCAGGACGGAACGTTGCTGGGCTACTCGCTATTGCAGGAGTCGGTGGATCAGGCCAGCTATATGTACAGCGATAACCATTATCTGGCGGAGATGGCAACCATCCTCGGTAAGCCagaa                                                                                                                                           >  1:3427627/1‑150 (MQ=255)

            gACTGGACGGTGAAATTCGCCGAAAACCGCAGTCAGGACGGAACGTTGCTGGGCTACTCGCTATTGCAGGAGTCGGTGGATCAGGCCAGCTATATGTACAGCGATAACCATTATCTGGCGGAGATGGCAACCATCCTCGGTAAGCCagaa                                                                                                                                           >  2:2080688/1‑150 (MQ=255)

             aCTGGACGGTGAAATTCGCCGAAAACCGCAGTCAGGACGGAACGTTGCTGGGCTACTCGCTATTGCAGGAGTCGGTGGATCAGGCCAGCTATATGTACAGCGATAACCATTATCTGGCGGAGATGGCAACCATCCTCGGTAAGCCagaag                                                                                                                                          <  2:4601004/150‑1 (MQ=255)

              cTGGACGGTGAAATTCGCCGAAAACCGCAGTCAGGACGGAACGTTGCTGGGCTACTCGCTATTGCAGGAGTCGGTGGATCAGGCCAGCTATATGTACAGCGATAACCATTATCTGGCGGAGATGGCAACCATCCTCGGTAAGCCagaaga                                                                                                                                         >  2:1053133/1‑150 (MQ=255)

              cTGGACGGTGAAATTCGCCGAAAACCGCAGTCAGGACGGAACGTTGCTGGGCTACTCGCTATTGCAGGAGTCGGTGGATCAGGCCAGCTATATGTACAGCGATAACCATTATCTGGCGGAGATGGCAACCATCCTCGGTAAGCCagaaga                                                                                                                                         >  2:4105943/1‑150 (MQ=255)

               tGGACGGTGAAATTCGCCGAAAACCGCAGTCAGGACGGAACGTTGCTGGGCTACTCGCTATTGCAGGAGTCGGTGGATCAGGCCAGCTATATGTACAGCGATAACCATTATCTGGCGGAGATGGCAACCATCCTCGGTAAGCCAGAagag                                                                                                                                        >  1:1566515/1‑150 (MQ=255)

                  aCGGTGAAATTCGCCGAAAACCGCAGTCAGGACGGAACGTTGCTGGGCTACTCGCTATTGCAGGAGTCGGTGGATCAGGCCAGCTATATGTACAGCGATAACCATTATCTGGCGGAGATGGCAACCATCCTCGGTAAGCCAGAAGAGGcc                                                                                                                                     >  2:3202858/1‑150 (MQ=255)

                  aCGGTGAAATTCGCCGAAAACCGCAGTCAGGACGGAACGTTGCTGGGCTACTCGCTATTGCAGGAGTCGGTGGATCAGGCCAGCTATATGTACAGCGATAACCATTATCTGGCGGAGATGGCAACCATCCTAGGTAAGCCAGAAGAGGcc                                                                                                                                     <  2:3678472/150‑1 (MQ=255)

                     gTGAAATTCGCCGAAAACCGCAGTCAGGACGGAACGTTGCTGGGCTACTCGCTATTGCAGGAGTCGGTGGATCAGGCCAGCTATATGTACAGCGATAACCATTATCTGGCGGAGATGGCAACCATCCTCGGTAAGCCAGAAGAGGCCaaa                                                                                                                                  >  2:4326587/1‑150 (MQ=255)

                       gAAATTCGCCGAAAACCGCAGTCAGGACGGAACGTTGCTGGGCTACTCGCTATTGCAGGAGTCGGTGGATCAGGCCAGCTATATGTACAGCGATAACCATTATCTGGCGGAGATGGCAACCATCCTCGGTAAGCCAGAAGAGGCCAAACg                                                                                                                                <  2:3879102/150‑1 (MQ=255)

                        aaaTTCGCCGAAAACCGCAGTCAGGACGGAACGTTGCTGGGCTACTCGCTATTGCAGGAGTCGGTGGATCAGGCCAGCTATATGTACAGCGATAACCATTATCTGGCGGAGATGGCAACCATCCTCGGTAAGCCAGAAGAGGCCAAACGc                                                                                                                               >  2:4024558/1‑150 (MQ=255)

                              gCCGAAAACCGCAGTCAGGACGGAACGTTGCTGGGCTACTCGCTATTGCAGGAGTCGGTGGATCAGGCCAGCTATATGTACAGCGATAACCATTATCTGGCGGAGATGGCAACCATCCTCGGTAAGCCAGAAGAGGCCAAACGCTATCGc                                                                                                                         <  1:2206955/150‑1 (MQ=255)

                                cGAAAACCGCAGTCAGGACGGAACGTTGCTGGGCTACTCGCTATTGCAGGAGTCGGTGGATCAGGCCAGCTATATGTACAGCGATAACCATTATCTGGCGGAGATGGCAACCATCCTCGGTAAGCCAGAAGAGGCCAAACGCTATCGCCa                                                                                                                       <  1:2144726/150‑1 (MQ=255)

                                cGAAAACCGCAGTCAGGACGGAACGTTGCTGGGCTACTCGCTATTGCAGGAGTCGGTGGATCAGGCCAGCTATATGTACAGCGATAACCATTATCTGGCGGAGATGGCAACCATCCTCGGTAAGCCAGAAGAGGCCAAACGCTATCGCCa                                                                                                                       >  1:3082716/1‑150 (MQ=255)

                                 gAAAACCGCAGTCAGGACGGAACGTTGCTGGGCTACTCGCTATTGCAGGAGTCGGTGGATCAGGCCAGCTATATGTACAGCGATAACCATTATCTGGCGGAGATGGCAACCATCCTCGGTAAGCCAGAAGAGGCCAAACGCTATCGCCAg                                                                                                                      <  1:1835018/150‑1 (MQ=255)

                                  aaaaCCGCAGTCAGGACGGAACGTTGCTGGGCTACTCGCTATTGCAGGAGTCGGTGGATCAGGCCAGCTATATGTACAGCGATAACCATTATCTGGCGGAGATGGCAACCATCCTAGGTAAGCCAGAAGAGGCCAAACGCTATCGCCAGt                                                                                                                     <  2:4804458/150‑1 (MQ=255)

                                    aaCCGCAGTCAGGACGGAACGTTGCTGGGCTACTCGCTATTGCAGGAGTCGGTGGATCAGGCCAGCTATATGTACAGCGATAACCATTATCTGGCGGAGATGGCAACCATCCTCGGTAAGCCAGAAGAGGCCAAACGCTATCGCCAGTTg                                                                                                                   <  2:2627811/150‑1 (MQ=255)

                                        gCAGTCAGGACGGAACGTTGCTGGGCTACTCGCTATTGCAGGAGTCGGTGGATCAGGCCAGCTATATGTACAGCGATAACCATTATCTGGCGGAGATGGCAACCATCCTCGGTAAGCCAGAAGAGGCCTAACGCTATCGCCAGTTGGcac                                                                                                               >  2:2157656/1‑150 (MQ=255)

                                        gCAGTCAGGACGGAACGTTGCTGGGCTACTCGCTATTGCAGGAGTCGGTGGATCAGGCCAGCTATATGTACAGCGATAACCATTATCTGGCGGAGATGGCAACCATCCTCGGTAAGCCAGAAGAGGCCAAACGCTATCGCCAGTTGGcac                                                                                                               >  1:1157937/1‑150 (MQ=255)

                                         cAGTCAGGACGGAACGTTGCTGGGCTACTCGCTATTGCAGGAGTCGGTGGATCAGGCCAGCTATATGTACAGCGATAACCATTATCTGGCGGAGATGGCAACCATCCTCGGTAAGCCAGAAGAGGCCAAACGCTATCGCCAGTTGGcaca                                                                                                              >  2:1797214/1‑150 (MQ=255)

                                          aGTCAGGACGGAACGTTGCTGGGCTACTCGCTATTGCAGGAGTCGGTGGATCAGGCCAGCTATATGTACAGCGATAACCATTATCTGGCGGAGATGGCAACCATCCTCGGTAAGCCAGAAGAGGCCAAACGCTATCGCCAGTTGGCAcag                                                                                                             >  1:4814901/1‑150 (MQ=255)

                                           gTCAGGACGGAACGTTGCTGGGCTACTCGCTATTGCAGGAGTCGGTGGATCAGGCCAGCTATATGTACAGCGATAACCATTATCTGGCGGAGATGGCAACCATCCTCGGTAAGCCAGAAGAGGCCAAACGCTATCGCCAGTTGGCAcagc                                                                                                            >  2:1183985/1‑150 (MQ=255)

                                            tCAGGACGGAACGTTGCTGGGCTACTCGCTATTGCAGGAGTCGGTGGATCAGGCCAGCTATCTGTACAGCGATAACCATTATCTGGCGGAGATGGCAACCATCCTCGGTAAGCCAGATGAGGCCAAACGCTATCGCCAGTTGGCAcagca                                                                                                           <  1:5135315/150‑1 (MQ=255)

                                            tCAGGACGGAACGTTGCTGGGCTACTCGCTATTGCAGGAGTCGGTGGATCAGGCCAGCTATATGTACAGCGATAACCATTATCTGGCGGAGATGGCAACCATCCTCGGTAAGCCAGAAGAGGCCAAACGCTATCGCCAGTTGGCAcagca                                                                                                           <  2:2975847/150‑1 (MQ=255)

                                            tCAGGACGGAACGTTGCTGGGCTACTCGCTATTGCAGGAGTCGGTGGATCAGGCCAGCTATATGTACAGCGATAACCATTATCTGGCGGAGATGGCAACCATCCTCGGTAAGCCAGAAGAGGCCAAACGCTATCGCCAGTTGGCAcagca                                                                                                           <  2:1918339/150‑1 (MQ=255)

                                               ggACGGAACGTTGCTGGGCTACTCGCTATTGCAGGAGTCGGTGGATCAGGCCAGCTATATGTACAGCGATAACCATTATCTGGCGGAGATGGCAACCATCCTCGGTAAGCCAGAAGAGGCCAAACGCTATCGCCAGTTGGCACAGCAGCt                                                                                                        >  2:5126965/1‑150 (MQ=255)

                                                gACGGAACGTTGCTGGGCTACTCGCTATTGCAGGAGTCGGTGGATCAGGCCAGCTATATGTACAGCGATAACCATTATCTGGCGGAGATGGCAACCATCCTCGGTAAGCCAGAAGAGGCCAAACGCTATCGCCAGTTGGCACAGCAGCTc                                                                                                       >  2:2987556/1‑150 (MQ=255)

                                                 aCGGAACGTTGCTGGGCTACTCGCTATTGCAGGAGTCGGTGGATCAGGCCAGCTATATGTACAGCGATAACCATTATCTGGCGGAGATGGCAACCATCCTCGGTAAGCCAGAAGAGGCCAAACGCTCTCGCCAGTTGGCACAGCAGCTcg                                                                                                      >  2:2077541/1‑150 (MQ=255)

                                                  cGGAACGTTGCTGGGCTACTCGCTATTGCAGGAGTCGGTGGATCAGGCCAGCTATATGTACAGCGATAACCATTATCTGGCGGAGATGGCAACCATCCTCGGTAAGCCAGAAGAGGCCAAACGCTATCGCCAGTTGGCACAGCAGCTcgc                                                                                                     >  2:1606594/1‑150 (MQ=255)

                                                  cGGAACGTTGCTGGGCTACTCGCTATTGCAGGAGTCGGTGGATCAGGCCAGCTATATGTACAGCGATAACCATTATCTGGCGGAGATGGCAACCATCCTCGGTAAGCCAGAAGAGGCCAAACGCTATCGCCAGTTGGCACAGCAGCTcgc                                                                                                     <  2:3297609/150‑1 (MQ=255)

                                                      aCGTTGCTGGGCTACTCGCTATTGCAGGAGTCGGTGGATCAGGCCAGCTATATGTACAGCGATAACCATTATCTGGCGGAGATGGCAACCATCCTCGGTAAGCCAGAAGAGGCCAAACGCTATCGCCAGTTGGCACAGCAGCTCGCGGAc                                                                                                 <  2:4214423/150‑1 (MQ=255)

                                                            cTGGGCTACTCGCTATTGCAGGAGTCGGTGGATCAGGCCAGCTATATGTACAGCGATAACCATTATCTGGCGGAGATGGCAACCATCCTAGGTAAGCCAGAAGAGGCCAAACGCTATCGCCAGTTGGCACAGCAGCTCGCGGACTACATc                                                                                           >  1:2434291/1‑150 (MQ=255)

                                                             tGGGCTACTCGCTATTGCAGGAGTCGGTGGATCAGGCCAGCTATATGTACAGCGATAACCATTATCTGGCGGAGATGGCAACCATCCTCGGTAAGCCAGAAGAGGCCAAACGCTATCGCCAGTTGGCACAGCAGCTCGCGGACTACATCa                                                                                          >  1:3558252/1‑150 (MQ=255)

                                                                gCTACTCGCTATTGCAGGAGTCGGTGGATCAGGCCAGCTATATGTACAGCGATAACCATTATCTGGCGGAGATGGCAACCATCCTCGGTAAGCCAGAAGAGGCCAAACGCTATCGCCAGTTGGCACAGCAGCTCGCGGACTACATCAaca                                                                                       <  2:2192229/150‑1 (MQ=255)

                                                                gCTACTCGCTATTGCAGGAGTCGGTGGATCAGGCCAGCTATATGTACAGCGATAACCATTATCTGGCGGAGATGGCAACCATCCTCGGTAAGCCAGAAGAGGCCAAACGCTATCGCCAGTTGGCACAGCAGCTCGCGGACTACATCAaca                                                                                       <  2:258396/150‑1 (MQ=255)

                                                                 cTACTCGCTATTGCAGGAGTCGGTGGATCAGGCCAGCTATATGTACAGCGATAACCATTATCTGGCGGAGATGGCAACCATCCTCGGTAAGCCAGAAGAGGCCAAACGCTATCGCCAGTTGGCACAGCAGCTCGCGGACTACATCAacac                                                                                      >  1:1851165/1‑150 (MQ=255)

                                                                  tACTCGCTATTGCAGGAGTCGGTGGATCAGGCCAGCTATATGTACAGCGATAACCATTATCTGGCGGAGATGGCAACCATCCTCGGTAAGCCAGAAGAGGCCAAACGCTATCGCCAGTTGGCACAGCAGCTCGCGGACTACATCAACAcc                                                                                     >  1:78414/1‑150 (MQ=255)

                                                                   aCTCGCTATTGCAGGAGTCGGTGGATCAGGCCAGCTATATGTACAGCGATAACCATTATCTGGCGGAGATGGCAACCATCCTCGGTAAGCCAGAAGAGGCCAAACGCTATCGCCAGTTGGCACAGCAGCTCGCGGACTACATCAACACCt                                                                                    <  2:1035677/150‑1 (MQ=255)

                                                                    cTCGCTATTGCAGGAGTCGGTGGATCAGGCCAGCTATATGTACAGCGATAACCATTATCTGGCGGAGATGGCAACCATCCTAGGTAAGCCAGAAGAGGCCAAACGCTATCGCCAGTTGGCACAGCAGCTCGCGGACTACATCAACACCTg                                                                                   <  1:3899914/150‑1 (MQ=255)

                                                                       gCTATTGCAGGAGTCGGTGGATCAGGCCAGCTATATGTACAGCGATAACCATTATCTGGCGGAGATGGCAACCATCCTCGGTAAGCCAGAAGAGGCCAAACGCTATCGCCAGTTGGCACAGCAGCTCGCGGACTACATCAACACCTGTAt                                                                                >  1:1094349/1‑150 (MQ=255)

                                                                         tATTGCAGGAGTCGGTGGATCAGGCCAGCTATATGTACAGCGATAACCATTATCTGGCGGAGATGGCAACCATCCTCGGTAAGCCAGAAGAGGCCAAACGCTATCGCCAGTTGGCACAGCAGCTCGCGGACTACATCAACACCTGTATGt                                                                              >  1:2921522/1‑150 (MQ=255)

                                                                            tGCAGGAGTCGGTGGATCAGGCCAGCTATATGTACAGCGATACCCATTATCTGGCGGAGATGGCAACCATCCTCGGTAAGCCAGAAGAGGCCAAACGCTATCGCCAGTTGGCACAGCAGCTCGCGGACTACATCAACACCTGTATGTTCg                                                                           <  2:4312976/150‑1 (MQ=255)

                                                                            tGCAGGAGTCGGTGGATCAGGCCAGCTATATGTACAGCGATAACCATTATCTGGCGGAGATGGCAACCATCCTCGGTAAGCCAGAAGAGGCCAAACGCTATCGCCAGTTGGCACAGCAGCTCGCGGACTACATCAACACCTGTATGTTCg                                                                           >  2:2284014/1‑150 (MQ=255)

                                                                               aGGAGTCGGTGGATCAGGCCAGCTATATGTACAGCGATAACCATTATCTGGCGGAGATGGCAACCATCCTCGGTAAGCCAGAAGAGGCCAAACGCTATCGCCAGTTGGCACAGCAGCTCGCGGACTACATCAACACCTGTATGTTCGAcc                                                                        <  2:1340555/150‑1 (MQ=255)

                                                                                 gAGTCGGTGGATCAGGCCAGCTATATGTACAGCGATAACCATTATCTGGCGGAGATGGCAACCATCCTCGGTAAGCCAGAAGAGGCCAAACGCTATCGCCAGTTGGCACAGCAGCTCGCGGACTACATCAACACCTGTATGTTCGACCCg                                                                      <  1:2839385/150‑1 (MQ=255)

                                                                                 gAGTCGGTGGATCAGGCCAGCTATATGTACAGCGATAACCATTATCTGGCGGAGATGGCAACCATCCTCGGTAAGCCAGAAGAGGCCAAACGCTATCGCCAGTTGGCACAGCAGCTCGCGGACTACATCAACACCTGTATGTTCGACCCg                                                                      <  2:1671449/150‑1 (MQ=255)

                                                                                   gTCGGTGGATCAGGCCAGCTATATGTACAGCGATAACCATTATCTGGCGGAGATGGCAACCATCCTCGGTAAGCCAGAAGAGGCCAAACGCTATCGCCAGTTGGCACAGCAGCTCGCGGACTACATCAACACCTGTATGTTCGACCCGAc                                                                    <  2:848988/150‑1 (MQ=255)

                                                                                   gTCGGTGGATCAGGCCAGCTATATGTACAGCGATAACCATTATCTGGCGGAGATGGCAACCATCCTCGGTAAGCCAGAAGAGGCCAAACGCTATCGCCAGTTGGCACAGCAGCTCGCGCACTACATCAACACCTGTATGTTCGACCCGAc                                                                    >  1:1397646/1‑150 (MQ=255)

                                                                                          gATCAGGCCAGCTATATGTACAGCGATAACCATTATCTGGCGGAGATGGCAACCATCCTCGGTAAGCCAGAAGAGGCCAAACGCTATCGCCAGTTGGCACAGCAGCTCGCGGACTACATCAACACCTGTATGTTCGACCCGACTACCCAg                                                             <  1:3619531/150‑1 (MQ=255)

                                                                                              aGGCCAGCTATATGTACAGCGATAACCATTATCTGGCGGAGATGGCAACCATCCTCGGTAAGCCAGAAGAGGCCAAACGCTATCGCCAGTTGGCACAGCAGCTCGCGGACTACATCAACACCTGTATGTTCGACCCGACTACCCAGTTct                                                         <  1:1011940/150‑1 (MQ=255)

                                                                                              aGGCCAGCTATATGTACAGCGATAACCATTATCTGGCGGAGATGGCAACCATCCTCGGTAAGCCAGAAGAGGCCAAACGCTATCGCCAGTTGGCACAGCAGCTCGCGGACTACATCAACACCTGTATGTTCGACCCGACTACCCAGTTct                                                         <  2:1366101/150‑1 (MQ=255)

                                                                                              aGGCCAGCTATATGTACAGCGATAACCATTATCTGGCGGAGATGGCAACCATCCTAGGTAAGCCAGAAGAGGCCAAACGCTATCGCCAGTTGGCACAGCAGCTCGCGGACTACATCAACACCTGTATGTTCGACCCGACTACCCAGTTct                                                         <  2:4540701/150‑1 (MQ=255)

                                                                                                   aGCTATATGTACAGCGATAACCATTATCTGGCGGAGATGGCAACCATCCTCGGTAAGCCAGAAGAGGCCAAACGCTATCGCCAGTTGGCACAGCAGCTCGCGGACTACATCAACACCTGTATGTTCGACCCGACTACCCAGTTCTACTAt                                                    >  2:3922882/1‑150 (MQ=255)

                                                                                                   aGCTATATGTACAGCGATAACCATTATCTGGCGGAGATGGCAACCATCCTCGGTAAGCCAGAAGAGGCCAAACGCTATCGCCAGTTGGCACAGCAGCTCGCGGACTACATCAACACCTGTATGTTCGACCCGACTACCCAGTTCTACTAt                                                    <  2:3427627/150‑1 (MQ=255)

                                                                                                   aGCTATATGTACAGCGATAACCATTATCTGGCGGAGATGGCAACCATCCTCGGTAAGCCAGAAGAGGCCAAACGCTATCGCCAGTTGGCACAGCAGCTCGCGGACTACATCAACACCTGTATGTTCGACCCGACTACCCAGTTCTACTAt                                                    <  1:4011690/150‑1 (MQ=255)

                                                                                                      tataTGTACAGCGATAACCATTATCTGGCGGAGATGGCAACCATCCTCGGTAAGCCAGAAGAGGCCAAACGCTATCGCCAGTTGGCACAGCAGCTCGCGGACTACATCAACACCTGTATGTTCGACCCGACTACCCAGTTCTACTATGac                                                 >  1:58894/1‑150 (MQ=255)

                                                                                                       atatGTACAGCGATAACCATTATCTGGCGGAGATGGCAACCATCCTCGGTAAGCCAGAAGAGGCCAAACGCTATCGCCAGTTGGCACAGCAGCTCGCGGACTACATCAACACCTGTATGTTCGACCCGACTACCCAGTTCTACTATGacg                                                >  2:4998140/1‑150 (MQ=255)

                                                                                                        tatGTACAGCGATAACCATTATCTGGCGGAGATGGCAACCATCCTCGGTAAGCCAGAAGAGGCCAAACGCTATCGCCAGTTGGCACAGCAGCTCGCGGACTACATCAACACCTGTATGTTCGACCCGACTACCCAGTTCTACTATGacgt                                               >  1:4260618/1‑150 (MQ=255)

                                                                                                         atGTACAGCGATAACCATTATCTGGCGGAGATGGCAACCATCCTCGGTAAGCCAGAAGAGGCCAAACGCTATCGCCAGTTGGCACAGCAGCTCGCGGACTACATCAACACCTGTATGTTCGACCCGACTACCCAGTTCTACTATGacgta                                              <  2:3380958/150‑1 (MQ=255)

                                                                                                         atGTACAGCGATAACCATTATCTGGCGGAGATGGCAACCATCCTCGGTAAGCCAGAAGAGGCCAAACGCTATCGCCAGTTGGCACAGCAGCTCGCGGACTACATCAACACCTGTATGTTCGACCCGACTACCCAGTTCTACTATGacgta                                              >  1:3131843/1‑150 (MQ=255)

                                                                                                         atGTACAGCGATAACCATTATCTGGCGGAGATGGCAACCATCCTCGGTAAGCCAGAAGAGGCCAAACGCTATCGCCAGTTGGCACAGCAGCTCGCGGACTACATCAACACCTGTATGTTCGACCCGACTACCCAGTTCTACTATGacgta                                              >  1:40858/1‑150 (MQ=255)

                                                                                                          tGTACAGCGATAACCATTATCTGGCGGAGATGGCAACCATCCTCGGTAAGCCAGAAGAGGCCAAACGCTATCGCCAGTTGGCACAGCAGCTCGCGGACTACATCAACACCTGTATGTTCGACCCGACTACCCAGTTCTACTATGacgtac                                             <  2:1131503/150‑1 (MQ=255)

                                                                                                           gTACAGCGATAACCATTATCTGGCGGAGATGGCAACCATCCTAGGTAAGCCAGAAGAGGCCAAACGCTATCGCCAGTTGGCACAGCAGCTCGCGGACTACATCAACACCTGTATGTTCGACCCGACTACCCAGTTCTACTATGacgtacg                                            >  2:2746521/1‑150 (MQ=255)

                                                                                                            tACAGCGATAACCATTATCTGGCGGAGATGGCAACCATCCTCGGTAAGCCAGAAGAGGCCAAACGCTATCGCCAGTTGGCACAGCAGCTCGCGGACTACATCAACACCTGTATGTTCGACCCGACTACCCAGTTCTACTATGacgtacgt                                           >  2:2547876/1‑150 (MQ=255)

                                                                                                               aGCGATAACCATTATCTGGCGGAGATGGCAACCATCCTCGGTAAGCCAGAAGAGGCCAAACGCTATCGCCAGTTGGCACAGCAGCTCGCGGACTACATCAACACCTGTATGTTCGACCCGACTACCCAGTTCTACTATGACGTACGTAtt                                        >  1:168501/1‑150 (MQ=255)

                                                                                                               aGCGATAACCATTATCTGGCGGAGATGGCAACCATCCTCGGTAAGCCAGAAGAGGCCAAACGCTATCGCCAGTTGGCACAGCAGCTCGCGGACTACATCAACACCTGTATGTTCGACCCGACTACCCAGTTCTACTATGACGTACGTAta                                        >  2:1768534/1‑149 (MQ=255)

                                                                                                                 cGATAACCATTATCTGGCGGAGATGGCAACCATCCTCGGTAAGCCAGAAGAGGCCAAACGCTATCGCCAGTTGGCACAGCAGCTCGCGGACTACATCAACACCTGTATGTTCGACCCGACTACCCAGTTCTACTATGACGTACGTATTGa                                      >  2:1652314/1‑150 (MQ=255)

                                                                                                                    tAACCATTATCTGGCGGAGATGGCAACCATCCTCGGTAAGCCAGAAGAGGCCAAACGCTATCGCCAGTTGGCACAGCAGCTCGCGGACTACATCAACACCTGTATGTTCGACCCGACTACCCAGTTCTACTATGACGTACGTATTGAAGa                                   <  2:3819139/150‑1 (MQ=255)

                                                                                                                         aTTATCTGGCGGAGATGGCAACCATCCTCGGTAAGCCAGAAGAGGCCAAACGCTATCGCCAGTTGGCACAGCAGCTCGCGGACTACATCAACACCTGTATGTTCGACCCGACTACCCAGTTCTACTATGACGTACGTATTGAAGATAAAc                              <  1:4510267/150‑1 (MQ=255)

                                                                                                                         aTTATCTGGCGGAGATGGCAACCATCCTCGGTAAGCCAGAAGAGGCCAAACGCTATCGCCAGTTGGCACAGCAGCTCGCGGACTACATCAACACCTGTATGTTCGACCCGACTACCCAGTTCTACTATGACGTACGTATTGAAGATAAAc                              <  2:3609861/150‑1 (MQ=255)

                                                                                                                          ttATCTGGCGGAGATGGCAACCATCCTCGGTAAGCCAGAAGAGGCCAAACGCTATCGCCAGTTGGCACAGCAGCTCGCGGACTACATCAACACCTGTATGTTCGACCCGACTACCCAGTTCTACTATGACGTACGTATTGAAGATAAAcc                             >  1:4849153/1‑150 (MQ=255)

                                                                                                                          ttATCTGGCGGAGATGGCAACCATCCTCGGTAAGCCAGAAGAGGCCAAACGCTATCGCCAGTTGGCACAGCAGCTCGCGGACTACATCAACACCTGTATGTTCGACCCGACTACCCAGTTCTACTATGACGTACGGATTGAAGATAAAcc                             >  2:4929576/1‑150 (MQ=255)

                                                                                                                           tATCTGGCGGAGATGGCAACCATCCTAGGTAAGCCAGAAGAGGCCAAACGCTATCGCCAGTTGGCACAGCAGCTCGCGGACTACATCAACACCTGTATGTTCGACCCGACTACCCAGTTCTACTATGACGTACGTATTGAAGATAAACCa                            <  1:549974/150‑1 (MQ=255)

                                                                                                                             tCTGGCGGAGATGGCAACCATCCTCGGTAAGCCAGAAGAGGCCAAACGCTATCGCCAGTTGGCACAGCAGCTCGCGGACTACATCAACACCTGTATGTTCGACCCGACTACCCAGTTCTACTATGACGTACGTATTGAAGATAAACCACt                          >  2:4973249/1‑150 (MQ=255)

                                                                                                                             tCTGGCGGAGATGGCAACCATCCTCGGTAAGCCAGAAGAGGCCAAACGCTATCGCCAGTTGGCACAGCAGCTCGCGGACTACATCAACACCTGTATGTTCGACCCGACTACCCAGTTCTACTATGACGTACGTATTGAAGATAAACCACt                          <  1:1270981/150‑1 (MQ=255)

                                                                                                                              cTGGCGGAGATGGCAACCATCCTCGGTAAGCCAGAAGAGGCCAAACGCTATCGCCAGTTGGCACAGCAGCTCGCGGACTACATCAACACCTGTATGTTCGACCCGACTACCCAGTTCTACTATGACGTACGTATTGAAGATAAACCACTg                         <  1:4560705/150‑1 (MQ=255)

                                                                                                                                ggCGGAGATGGCAACCATCCTCGGTAAGCCAGAAGAGGCCAAACGCTATCGCCAGTTGGCACAGCAGCTCGCGGACTACATCAACACCTGTATGTTCGACCCGACTACCCAGTTCTACTATGACGTACGTATTGAAGATAAACCACTGGc                       >  1:5093588/1‑150 (MQ=255)

                                                                                                                                ggCGGAGATGGCAACCATCCTCGGTAAGCCAGAAGAGGCCAAACGCTATCGCCAGTTGGCACAGCAGCTCGCGGACTACATCAACACCTGTATGTTCGACCCGACTACCCAGTTCTACTATGACGTACGTATTGAAGATAAACCACTGGc                       <  2:1723756/150‑1 (MQ=255)

                                                                                                                                  cGGAGATGGCAACCATCCTAGGTAAGCCAGAAGAGGCCAAACGCTATCGCCAGTTGGCACAGCAGCTCGCGGACTACATCAACACCTGTATGTTCGACCCGACTACCCAGTTCTACTATGACGTACGTATTGAAGATAAACCACTGGCGa                     <  2:328986/150‑1 (MQ=255)

                                                                                                                                   ggAGATGGCAACCATTCTCGGTAAGCCAGAAGAGGCCAAACGCTATCGCCAGTTGGCACAGCAGCTCGCGGACTACATCAACACCTGTATGTTCGACCCGACTACCCAGTTCTACTATGACGTACGTATTGAAGATAAACCACTGGCGaa                    >  1:5087034/1‑150 (MQ=255)

                                                                                                                                   ggAGATGGCAACCATCCTCGGTAAGCCAGAAGAGGCCAAACGCTATCGCCAGTTGGCACAGCAGCTCGCGGACTACATCAACACCTGTATGTTCGACCCGACTACCCAGTTCTACTATGACGTACGTATTGAAGATAAACCACTGGCGaa                    >  1:4474672/1‑150 (MQ=255)

                                                                                                                                    gagaTGGCAACCATCCTCGGTAAGCCAGAAGAGGCCAAACGCTATCGCCAGTTGGCACAGCAGCTCGCGGACTACATCAACACCTGTATGTTCGACCCGACTACCCAGTTCTACTATGACGTACGTATTGAAGATAAACCACTGGCGAAc                   >  1:1253118/1‑150 (MQ=255)

                                                                                                                                     agaTGGCAACCATCCTCGGTAAGCCAGAAGAGGCCAAACGCTATCGCCAGTTGGCACAGCAGCTCGCGGACTACATCAACACCTGTATGTTCGACCCGACTACCCAGTTCTACTATGACGTACGTATTGAAGATAAACCACTGGCGAACg                  <  1:853507/150‑1 (MQ=255)

                                                                                                                                         ggCAACCATCCTCGGTAAGCCAGAAGAGGCCAAACGCTATCGCCAGTTGGCACAGCAGCTCGCGGACTACATCAACACCTGTATGTTCGACCCGACTACCCAGTTCTACTATGACGTACGTATTGAAGATAAACCACTGGCGAACGGCTg              >  1:3676868/1‑150 (MQ=255)

                                                                                                                                           cAACCATCCTCGGTAAGCCAGAAGAGGCCAAACGCTATCGCCAGTTGGCACAGCAGCTCGCGGACTACATCAACACCTGTATGTTCGACCCGACTACCCAGTTCTACTATGACGTACGTATTGAAGATAAACCACTGGCGAACGGCTgcg            <  1:2296227/150‑1 (MQ=255)

                                                                                                                                            aaCCATCCTCGGTAAGCCAGAAGAGGCCAAACGCTATCGCCAGTTGGCACAGCAGCTCGCGGACTACATCAACACCTGTATGTTCGACCCGACTACCCAGTTCTACTATGACGTACGTATTGAAGATAAACCACTGGCGAACGGCTgcgc           >  2:3090488/1‑150 (MQ=255)

                                                                                                                                              ccATCCTCGGTAAGCCAGAAGAGGCCAAACGCTATCGCCAGTTGGCACAGCAGCTCGCGGACTACATCAACACCTGTATGTTCGACCCGACTACCCAGTTCTACTATGACGTACGTATTGAAGATAAACCACTGGCGAACGGCTGCGCgg         <  2:1434684/150‑1 (MQ=255)

                                                                                                                                                 tCCTCGGTAAGCCAGAAGAGGCCAAACGCTATCGCCAGTTGGCACAGCAGCTCGCGGACTACATCAACACCTGTATGTTCGACCCGACTACCCAGTTCTACTATGACGTACGTATTGAAGATAAACCACTGGCGAACGGCTGCGCGGGCa      >  2:710473/1‑150 (MQ=255)

                                                                                                                                                 tCCTAGGTAAGCCAGAAGAGGCCAAACGCTATCGCCAGTTGGCACAGCAGCTCGCGGACTACATCAACACCTGTATGTTCGACCCGACTACCCAGTTCTACTATGACGTACGTATTGAAGATAAACCACTGGCGAACGGCTGCGCGGGCa      >  1:2653747/1‑150 (MQ=255)

                                                                                                                                                  ccTCGGTAAGCCAGAAGAGGCCAAACGCTATCGCCAGTTGGCACAGCAGCTCGCGGACTACATCAACACCTGTATGTTCGACCCGACTACCCAGTTCTACTATGACGTACGTATTGAAGATAAACCACTGGCGAACGGCTGCGCGGGCaa     <  1:3751565/150‑1 (MQ=255)

                                                                                                                                                    tCGGTAAGCCAGAAGAGGCCAAACGCTATCGCCAGTTGGCACAGCAGCTCGCGGACTACATCAACACCTGTATGTTCGACCCGACTACCCAGTTCTACTATGACGTACGTATTGAAGATAAACCACTGGCGAACGGCTGCGCGGGCAAAc   <  1:2593702/150‑1 (MQ=255)

GGCAAACGTAGCGACTGGACGGTGAAATTCGCCGAAAACCGCAGTCAGGACGGAACGTTGCTGGGCTACTCGCTATTGCAGGAGTCGGTGGATCAGGCCAGCTATATGTACAGCGATAACCATTATCTGGCGGAGATGGCAACCATCCTCGGTAAGCCAGAAGAGGCCAAACGCTATCGCCAGTTGGCACAGCAGCTCGCGGACTACATCAACACCTGTATGTTCGACCCGACTACCCAGTTCTACTATGACGTACGTATTGAAGATAAACCACTGGCGAACGGCTGCGCGGGCAAACC  >  CP009273/3223879‑3224177

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`